deuterium oxide

Summary

Summary: The isotopic compound of hydrogen of mass 2 (deuterium) with oxygen. (From Grant & Hackh's Chemical Dictionary, 5th ed) It is used to study mechanisms and rates of chemical or nuclear reactions, as well as biological processes.

Top Publications

  1. Busch R, Neese R, Awada M, Hayes G, Hellerstein M. Measurement of cell proliferation by heavy water labeling. Nat Protoc. 2007;2:3045-57 pubmed
    ..The protocols enable definitive in vivo studies of the fraction or absolute number of newly divided cells and their subsequent survival kinetics in animals and humans...
  2. Wang B, Sun G, Anderson D, Jia M, Previs S, Anderson V. Isotopologue distributions of peptide product ions by tandem mass spectrometry: quantitation of low levels of deuterium incorporation. Anal Biochem. 2007;367:40-8 pubmed
    ..The ability to determine the isotopic composition of sequential product ions permits the isotopic composition of individual amino acid residues in the precursor ion to be determined...
  3. Armstrong L, Klau J, Ganio M, McDermott B, Yeargin S, Lee E, et al. Accumulation of 2H2O in plasma and eccrine sweat during exercise-heat stress. Eur J Appl Physiol. 2010;108:477-82 pubmed publisher
    ..b>Deuterium oxide ((2)H(2)O) accumulation in plasma and eccrine sweat was measured at two sites (back and forehead)...
  4. Tynkkynen T, Tiainen M, Soininen P, Laatikainen R. From proton nuclear magnetic resonance spectra to pH. Assessment of 1H NMR pH indicator compound set for deuterium oxide solutions. Anal Chim Acta. 2009;648:105-12 pubmed publisher
    ..Also, when the ionic strength is known with accuracy better than 0.1 mol dm(-3) and the solute concentrations are low, pH(nmr)(*) (the NMR estimate of pH) can be assumed to be within 0.05 pH units from potentiometrically determined pH...
  5. Erdogan G, Grotjahn D. Mild and selective deuteration and isomerization of alkenes by a bifunctional catalyst and deuterium oxide. J Am Chem Soc. 2009;131:10354-5 pubmed publisher
    ..coordinated to the CpRu center; at this stage the protonated nitrogen could undergo H/D exchange with deuterium oxide. An exceptional degree of deuteration is achieved at positions accessible to isomerization, with a high ..
  6. Sen A, Balamurugan V, Rajak K, Chakravarti S, Bhanuprakash V, Singh R. Role of heavy water in biological sciences with an emphasis on thermostabilization of vaccines. Expert Rev Vaccines. 2009;8:1587-602 pubmed publisher
    ..b>Deuterium oxide (heavy water; D(2)O) has previously been reported to have a protective effect on biomolecules (proteins and ..
  7. de Riva A, Deery M, McDonald S, Lund T, Busch R. Measurement of protein synthesis using heavy water labeling and peptide mass spectrometry: Discrimination between major histocompatibility complex allotypes. Anal Biochem. 2010;403:1-12 pubmed publisher
    ..It simplifies measurements of protein synthesis, enabling novel applications in physiology, in genotype/phenotype interactions, and potentially in kinetic proteomics...
  8. Leung V, Hung S, Li L, Wu E, Luk K, Chan D, et al. Age-related degeneration of lumbar intervertebral discs in rabbits revealed by deuterium oxide-assisted MRI. Osteoarthritis Cartilage. 2008;16:1312-8 pubmed publisher
    ..This study addresses whether rabbits undergo age-related disc degeneration, assessed using deuterium oxide-assisted magnetic resonance imaging (MRI) of the lumbar IVDs.
  9. Hirai K, Tomida M, Kikuchi Y, Ueda O, Ando H, Asanuma N. Effects of deuterium oxide on Streptococcus mutans and Pseudomonas aeruginosa. Bull Tokyo Dent Coll. 2010;51:175-83 pubmed
    ..It is well known that deuterium oxide (D?O) causes toxic effects on a number of biological systems...

More Information

Publications61

  1. Das A, Sinha S, Acharya B, Paul P, Bhattacharyya B, Chakrabarti G. Deuterium oxide stabilizes conformation of tubulin:a biophysical and biochemical study. BMB Rep. 2008;41:62-7 pubmed
    The present study was aimed to elucidate the mechanism of stabilization of tubulin by deuterium oxide (D(2)O)...
  2. Dugdale A, Curtis G, Milne E, Harris P, Argo C. Assessment of body fat in the pony: part II. Validation of the deuterium oxide dilution technique for the measurement of body fat. Equine Vet J. 2011;43:562-70 pubmed publisher
    ..An objective, minimally-invasive method to accurately quantify body fat in living animals is required to aid nutritional management and define welfare/performance limits...
  3. Xiao G, Garg M, Lim S, Wong D, Go V, Lee W. Determination of protein synthesis in vivo using labeling from deuterated water and analysis of MALDI-TOF spectrum. J Appl Physiol (1985). 2008;104:828-36 pubmed publisher
    ..The combined use of postsource decay sequence identification and mass isotopomer distribution analysis makes the use of in vivo labeling with deuterated water a precise method to determine specific protein synthesis...
  4. Mamonov A, Coalson R, Zeidel M, Mathai J. Water and deuterium oxide permeability through aquaporin 1: MD predictions and experimental verification. J Gen Physiol. 2007;130:111-6 pubmed
    ..The combined computational and experimental results suggest that deuterium oxide permeability through AQP1 is similar to that of water...
  5. Yang X, Chen W, Rendahl A, Hegeman A, Gray W, Cohen J. Measuring the turnover rates of Arabidopsis proteins using deuterium oxide: an auxin signaling case study. Plant J. 2010;63:680-95 pubmed publisher
    ..These considerations suggest a prescription for specific experimental designs that minimize interference resulting from the induction of treatment-specific gene expression in the results obtained...
  6. Currell K, Urch J, Cerri E, Jentjens R, Blannin A, Jeukendrup A. Plasma deuterium oxide accumulation following ingestion of different carbohydrate beverages. Appl Physiol Nutr Metab. 2008;33:1067-72 pubmed publisher
    ..Plasma and saliva samples were analyzed for deuterium enrichment by isotope ratio mass spectrometry. Deuterium oxide enrichments were compared using a 2-way repeated measures analysis of variance...
  7. Ramirez E, Valencia M, Bourges H, Espinosa T, Moya Camarena S, Salazar G, et al. Body composition prediction equations based on deuterium oxide dilution method in Mexican children: a national study. Eur J Clin Nutr. 2012;66:1099-103 pubmed publisher
    ..study was to facilitate assessments of body composition in Mexican school children of different geographical regions and ethnicity by developing equations for bioelectrical impedance and anthropometry based on deuterium oxide dilution.
  8. Matsuki H, Okuno H, Sakano F, Kusube M, Kaneshina S. Effect of deuterium oxide on the thermodynamic quantities associated with phase transitions of phosphatidylcholine bilayer membranes. Biochim Biophys Acta. 2005;1712:92-100 pubmed
    ..DPPC), distearoylphosphatidylcholine (DSPC) and dihexadecylphosphatidylcholine (DHPC), in deuterium oxide (D(2)O) and hydrogen oxide (H(2)O) were observed by differential scanning calorimetry (DSC) under ambient ..
  9. Previs S, Fatica R, Chandramouli V, Alexander J, Brunengraber H, Landau B. Quantifying rates of protein synthesis in humans by use of 2H2O: application to patients with end-stage renal disease. Am J Physiol Endocrinol Metab. 2004;286:E665-72 pubmed
    ..Estimates are in subjects with renal disease, but the method should be applicable to estimates of protein synthetic rates in normal subjects and in other pathological states...
  10. Macias N, Calderón de la Barca A, Bolaños A, Alemán H, Esparza J, Valencia M. Body composition in Mexican adults by air displacement plethysmography (ADP) with the BOD-POD and deuterium oxide dilution using infrared spectroscopy (IRS-DOD). Food Nutr Bull. 2002;23:99-102 pubmed
    ..for body density by air displacement plethysmography (ADP) with the BOD-POD, and for total body water by deuterium oxide dilution and infrared spectroscopy (IRS-DOD). Subjects were given a 30 g dose of deuterium oxide...
  11. Gardner J, Turner S, Bautista A, Lindwall G, Awada M, Hellerstein M. Measurement of liver collagen synthesis by heavy water labeling: effects of profibrotic toxicants and antifibrotic interventions. Am J Physiol Gastrointest Liver Physiol. 2007;292:G1695-705 pubmed
  12. Davies S, Spanel P, Smith D. Rapid measurement of deuterium content of breath following oral ingestion to determine body water. Physiol Meas. 2001;22:651-9 pubmed
    ..We conclude that FA-MS is a powerful new method that, when combined with oral D2O loading, enables measurement of the dispersal kinetics of HDO and the calculation of total body water within 2 hours...
  13. Norberg A, Sandhagen B, Bratteby L, Gabrielsson J, Jones A, Fan H, et al. Do ethanol and deuterium oxide distribute into the same water space in healthy volunteers?. Alcohol Clin Exp Res. 2001;25:1423-30 pubmed
    ..Ethanol concentration-time profiles were established for breath, venous blood, and urine. The values of Vss obtained for ethanol were compared with TBW determined by deuterium oxide dilution.
  14. White L, Ringo J, Dowse H. Effects of deuterium oxide and temperature on heart rate in Drosophila melanogaster. J Comp Physiol B. 1992;162:278-83 pubmed
    ..Heartbeat in this insect is an oscillation that is not temperature compensated. Deuterium oxide lengthens the period of a number of high and low frequency oscillators and clocks in a variety of organisms...
  15. Dufner D, Bederman I, Brunengraber D, Rachdaoui N, Ismail Beigi F, Siegfried B, et al. Using 2H2O to study the influence of feeding on protein synthesis: effect of isotope equilibration in vivo vs. in cell culture. Am J Physiol Endocrinol Metab. 2005;288:E1277-83 pubmed
    ..In particular, since there can be slow equilibration of 2H between water and alanine in the cell culture medium, special consideration must be made to avoid underestimating the rate of protein synthesis in vitro...
  16. Busch R, Kim Y, Neese R, Schade Serin V, Collins M, Awada M, et al. Measurement of protein turnover rates by heavy water labeling of nonessential amino acids. Biochim Biophys Acta. 2006;1760:730-44 pubmed
    ..In conclusion, 2H2O labeling permits sensitive, quantitative, operationally simple measurements of protein turnover in vivo by the rise-to-plateau approach, especially for proteins with slow constitutive turnover...
  17. Snodgrass J, Leonard W, Tarskaia L, Schoeller D. Total energy expenditure in the Yakut (Sakha) of Siberia as measured by the doubly labeled water method. Am J Clin Nutr. 2006;84:798-806 pubmed
    ..Although reduced physical activity has been implicated, few studies have accurately quantified energy expenditure in subsistence populations...
  18. Belloto E, Diraison F, Basset A, Allain G, Abdallah P, Beylot M. Determination of protein replacement rates by deuterated water: validation of underlying assumptions. Am J Physiol Endocrinol Metab. 2007;292:E1340-7 pubmed
    ..All of these results support (2)H(2)O as a safe, reliable, useful, and convenient tracer for studies of protein synthesis, including proteins with slow turnover rate...
  19. Riek A, Gerken M, Moors E. Measurement of milk intake in suckling llamas (Lama glama) using deuterium oxide dilution. J Dairy Sci. 2007;90:867-75 pubmed
    ..Milk intake in 11 suckling llamas was estimated from water kinetics using deuterium oxide (D2O) at d 17, 66, and 128 postpartum. Daily milk intakes averaged 2.6, 2.3, and 2...
  20. Cioni P, Strambini G. Effect of heavy water on protein flexibility. Biophys J. 2002;82:3246-53 pubmed
    ..Structure tightening by heavy water is generally amplified at higher temperatures, supporting a mostly hydrophobic nature of the underlying interaction, and under conditions that destabilize the globular fold...
  21. Uemura T, Moritake K, Akiyama Y, Kimura Y, Shingu T, Yamasaki T. Experimental validation of deuterium oxide-mediated antitumoral activity as it relates to apoptosis in murine malignant astrocytoma cells. J Neurosurg. 2002;96:900-8 pubmed
    b>Deuterium oxide (D2O), or heavy water, affects a variety of biological activities different from those of water...
  22. Jones J, Merritt M, Malloy C. Quantifying tracer levels of (2)H(2)O enrichment from microliter amounts of plasma and urine by (2)H NMR. Magn Reson Med. 2001;45:156-8 pubmed
    ..The measurement is insensitive to the presence of plasma protein, allowing direct analysis of (2)H(2)O enrichment from native plasma samples. Magn Reson Med 45:156-158, 2001...
  23. Decoursey T, Cherny V. Deuterium isotope effects on permeation and gating of proton channels in rat alveolar epithelium. J Gen Physiol. 1997;109:415-34 pubmed
    ..The effects of substituting deuterium oxide, D2O, for water, H2O, on both the conductance and the pH dependence of gating were explored...
  24. Kushner D, Baker A, Dunstall T. Pharmacological uses and perspectives of heavy water and deuterated compounds. Can J Physiol Pharmacol. 1999;77:79-88 pubmed
    ..Deuterated insecticides might well be more effective against resistant insects, but this potentially valuable possibility has not yet been studied...
  25. Gastaldelli A, Toschi E, Pettiti M, Frascerra S, Quiñones Galvan A, Sironi A, et al. Effect of physiological hyperinsulinemia on gluconeogenesis in nondiabetic subjects and in type 2 diabetic patients. Diabetes. 2001;50:1807-12 pubmed
    ..In type 2 diabetes, the excess GNG of the fasting state is carried over to the insulinized state, thereby contributing to glucose overproduction under both conditions...
  26. Mistlberger R, Marchant E, Kippin T. Food-entrained circadian rhythms in rats are insensitive to deuterium oxide. Brain Res. 2001;919:283-91 pubmed
    ..D(2)O may have utility as a screening test to identify putative food-entrainable pacemakers from among those central and peripheral tissues that can express circadian oscillations of clock genes independent of the SCN...
  27. Lindwall G, Hsieh E, Misell L, Chai C, Turner S, Hellerstein M. Heavy water labeling of keratin as a non-invasive biomarker of skin turnover in vivo in rodents and humans. J Invest Dermatol. 2006;126:841-8 pubmed
    ..In summary, 2H2O labeling of skin keratin represents a non-invasive approach for assessing skin turnover dynamics in pre-clinical models and in human subjects...
  28. Deurenberg Yap M, Deurenberg P. Validity of deuterium oxide dilution for the measurement of body fat among Singaporeans. Food Nutr Bull. 2002;23:34-7 pubmed
    ..fat percent (BF%) was measured in 108 adult Chinese, 76 Malays, and 107 Indians in Singapore by densitometry, deuterium oxide dilution (hydrometry), dual energy x-ray absorptiometry (DXA) and a chemical four-compartment model (BF%4c)...
  29. Sakurai Y, Kobayashi T. The medical-irradiation characteristics for neutron capture therapy at the Heavy Water Neutron Irradiation Facility of Kyoto University Research Reactor. Med Phys. 2002;29:2328-37 pubmed
    ..Besides, in the viewpoint of the radiation shielding for patient, it was confirmed that the whole-body exposure is effectively reduced by the new clinical collimators, compared with the old one...
  30. Valencia M, Aleman Mateo H, Salazar G, Hernandez Triana M. Body composition by hydrometry (deuterium oxide dilution) and bioelectrical impedance in subjects aged >60 y from rural regions of Cuba, Chile and Mexico. Int J Obes Relat Metab Disord. 2003;27:848-55 pubmed
    ..In Latin American and Caribbean countries such as Chile, Mexico and Cuba, the population over 60 y has increased steadily. In this age group, there is scarce information about body composition, particularly for those living in rural areas...
  31. Colley R, Byrne N, Hills A. Implications of the variability in time to isotopic equilibrium in the deuterium dilution technique. Eur J Clin Nutr. 2007;61:1250-5 pubmed
    ..To investigate the variability in isotopic equilibrium time under field conditions, and the impact of this variability on estimates of total body water (TBW) and body composition...
  32. Unno K, Hagima N, Kishido T, Okada S, Oku N. Deuterium-resistant algal cell line for D labeling of heterotrophs expresses enhanced level of Hsp60 in D2O medium. Appl Environ Microbiol. 2005;71:2256-9 pubmed
    ..Unno, T. Kishido, M. Morioka, S. Okada, and N. Oku, Biol. Pharm. Bull. 26:799-802, 2003). This suggests that the overexpression of heat shock proteins is required to compensate for the deuterium isotope effect...
  33. Hartmann J, Bader Y, Horvath Z, Saiko P, Grusch M, Illmer C, et al. Effects of heavy water (D2O) on human pancreatic tumor cells. Anticancer Res. 2005;25:3407-11 pubmed
    ..Since heavy water (deuterium oxide, D2O) was shown to be active in various cancer cell lines in vitro and in vivo, we now investigated its ..
  34. Allick G, van der Crabben S, Ackermans M, Endert E, Sauerwein H. Measurement of gluconeogenesis by deuterated water: the effect of equilibration time and fasting period. Am J Physiol Endocrinol Metab. 2006;290:E1212-7 pubmed
    ..After 5- and 15-h 2H2O incubation, GNG can be reliably measured with C5/2H2O. Gluconeogenetic labeling of glycogen did not affect the results after 3, 5, or 15 h of 2H2O incubation...
  35. Chan K, Gulati S, Edel J, de Mello A, Kazarian S. Chemical imaging of microfluidic flows using ATR-FTIR spectroscopy. Lab Chip. 2009;9:2909-13 pubmed publisher
  36. Zhang Y, Dood J, Beckstead A, Chen J, Li X, Burrows C, et al. Ultrafast excited-state dynamics and vibrational cooling of 8-oxo-7,8-dihydro-2'-deoxyguanosine in D2O. J Phys Chem A. 2013;117:12851-7 pubmed publisher
    ..4 ± 0.4 ps. Femtosecond time-resolved IR experiments provide additional insights into excited-state dynamics and the vibrational relaxation of several modes in the fingerprint region...
  37. Kim J, Kim M, Lee J. Role of moisture on the lipid oxidation determined by D(2)O in a linoleic acid model system. Food Chem. 2014;146:134-40 pubmed publisher
    ..as an active substance for lipid oxidation, was determined in linoleic acid model systems containing deuterium oxide (D2O) and deuterium free H2O, by measuring the headspace oxygen content and headspace volatiles...
  38. Heppenstall L, Strong R, Trevisan J, Martin F. Incorporation of deuterium oxide in MCF-7 cells to shed further mechanistic insights into benzo[a]pyrene-induced low-dose effects discriminated by ATR-FTIR spectroscopy. Analyst. 2013;138:2583-91 pubmed publisher
    ..b>Deuterium oxide (D2O) was then applied as part of a fixative solution and/or at low levels incorporated into growth medium ..
  39. Bahr S, Kempter V. Interaction of benzene with amorphous solid water adsorbed on polycrystalline Ag. J Chem Phys. 2007;127:074707 pubmed
    ..Films produced by codepositing benzene and D(2)O or by postdepositing D(2)O layers on benzene films display "volcano like" benzene desorption during ice crystallization...
  40. Hayes G, Simko J, Holochwost D, Kuchinsky K, Busch R, Misell L, et al. Regional cell proliferation in microdissected human prostate specimens after heavy water labeling in vivo: correlation with prostate epithelial cells isolated from seminal fluid. Clin Cancer Res. 2012;18:3250-60 pubmed publisher
    ..We describe here two novel in vivo metrics of cell proliferation in men with prostate neoplasia...
  41. Marmodée B, Jahn K, Ariese F, Gooijer C, Kumke M. Direct spectroscopic evidence of 8- and 9-fold coordinated europium(III) species in H(2)O and D(2)O. J Phys Chem A. 2010;114:13050-4 pubmed publisher
    ..On the basis of the spectroscopic analysis, the two species were attributed to 8- and 9-fold coordinated Eu(III) aquo ions...
  42. Cook G, Opella S. Secondary structure, dynamics, and architecture of the p7 membrane protein from hepatitis C virus by NMR spectroscopy. Biochim Biophys Acta. 2011;1808:1448-53 pubmed publisher
    ..A solid-state NMR two-dimensional separated local field spectrum of p7 aligned in phospholipid bilayers provided the tilt angles of two of these segments. A preliminary structural model of p7 derived from these NMR data is presented...
  43. Anderson J, Hern ndez G, LeMaster D. A billion-fold range in acidity for the solvent-exposed amides of Pyrococcus furiosus rubredoxin. Biochemistry. 2008;47:6178-88 pubmed publisher
  44. Pineda Sanabria S, Robertson I, Sykes B. Structure of trans-resveratrol in complex with the cardiac regulatory protein troponin C. Biochemistry. 2011;50:1309-20 pubmed publisher
    ..This has been seen for other troponin C-targeting inotropes and implicates the modulation of the troponin C-troponin I interaction as a possible mechanism of action for trans-resveratrol...
  45. Li J, Bian H, Chen H, Wen X, Hoang B, Zheng J. Ion association in aqueous solutions probed through vibrational energy transfers among cation, anion, and water molecules. J Phys Chem B. 2013;117:4274-83 pubmed publisher
  46. Veen S, Antoniuk O, Weber B, Potenza M, Mazzoni S, Schall P, et al. Colloidal aggregation in microgravity by critical Casimir forces. Phys Rev Lett. 2012;109:248302 pubmed
    ..By using the continuously variable particle interaction potential we can for the first time experimentally relate the strength of attraction between the particles and the structure of the aggregates...
  47. Kashiwazaki H, Uenishi K, Kobayashi T, Rivera J, Coward W, Wright A. Year-round high physical activity levels in agropastoralists of Bolivian Andes: results from repeated measurements of DLW method in peak and slack seasons of agricultural activities. Am J Hum Biol. 2009;21:337-45 pubmed publisher
    ..A flexible division of labor played an important role in the equitable energetic increase in both men and women...
  48. Rog T, Murzyn K, Milhaud J, Karttunen M, Pasenkiewicz Gierula M. Water isotope effect on the phosphatidylcholine bilayer properties: a molecular dynamics simulation study. J Phys Chem B. 2009;113:2378-87 pubmed publisher
    ..This large isotope effect has been assigned to the repercussions of the longer lived D-bonding to DPPC headgroups insofar as all water molecules sense the presence of the DPPC bilayer...
  49. Seto H, Yamada N, Nagao M, Hishida M, Takeda T. Bending modulus of lipid bilayers in a liquid-crystalline phase including an anomalous swelling regime estimated by neutron spin echo experiments. Eur Phys J E Soft Matter. 2008;26:217-23 pubmed publisher
    ..The hardening of a lipid bilayer upon approaching the main transition point in the anomalous swelling regime was observed, which naturally connects the bending modulus in the gel phase below the main transition temperature...
  50. Villars C, Bergouignan A, Dugas J, Antoun E, Schoeller D, Roth H, et al. Validity of combining heart rate and uniaxial acceleration to measure free-living physical activity energy expenditure in young men. J Appl Physiol (1985). 2012;113:1763-71 pubmed publisher
    ..Individual calibration of the HR/AEE relationship is necessary for AEE estimations at an individual level rather than at group scale and for ?AEE evaluation...
  51. Topham P, Glidle A, Toolan D, WEIR M, Skoda M, BARKER R, et al. The relationship between charge density and polyelectrolyte brush profile using simultaneous neutron reflectivity and in situ attenuated total internal reflection FTIR. Langmuir. 2013;29:6068-76 pubmed publisher
    ..This combined approach makes it possible to quantify the charge on a polymer brush alongside the morphology adopted by the polymer chains...
  52. Hernandez B, Pflüger F, Nsangou M, Ghomi M. Vibrational analysis of amino acids and short peptides in hydrated media. IV. Amino acids with hydrophobic side chains: L-alanine, L-valine, and L-isoleucine. J Phys Chem B. 2009;113:3169-78 pubmed publisher