cyanogen bromide

Summary

Summary: Cyanogen bromide (CNBr). A compound used in molecular biology to digest some proteins and as a coupling reagent for phosphoroamidate or pyrophosphate internucleotide bonds in DNA duplexes.

Top Publications

  1. Miller E, Lunde L. Isolation and characterization of the cyanogen bromide peptides from the alpha 1(II) chain of bovine and human cartilage collagen. Biochemistry. 1973;12:3153-9 pubmed
  2. Blonder J, Conrads T, Yu L, Terunuma A, Janini G, Issaq H, et al. A detergent- and cyanogen bromide-free method for integral membrane proteomics: application to Halobacterium purple membranes and the human epidermal membrane proteome. Proteomics. 2004;4:31-45 pubmed
    ..The present technique does not rely on the use of detergents, strong organic acids or cyanogen bromide-mediated proteolysis...
  3. Macmillan D, Arham L. Cyanogen bromide cleavage generates fragments suitable for expressed protein and glycoprotein ligation. J Am Chem Soc. 2004;126:9530-1 pubmed
    Herein cyanogen bromide is employed for the efficient production of N-terminal cysteine containing protein fragments for expressed protein ligation (EPL) from polyhistidine-tagged precursors...
  4. Sharpe S, Yau W, Tycko R. Expression and purification of a recombinant peptide from the Alzheimer's beta-amyloid protein for solid-state NMR. Protein Expr Purif. 2005;42:200-10 pubmed
    ..Initial structural data obtained by atomic force microscopy, electron microscopy, and solid-state NMR measurements of Abeta(11-26) fibrils are also presented...
  5. Henkel W, Dreisewerd K. Cyanogen bromide peptides of the fibrillar collagens I, III, and V and their mass spectrometric characterization: detection of linear peptides, peptide glycosylation, and cross-linking peptides involved in formation of homo- and heterotypic fibrils. J Proteome Res. 2007;6:4269-89 pubmed
    The network of the fibrillar collagens I, III, and V, extracted from fetal calf skin and cleaved with cyanogen bromide, was studied by means of ultraviolet matrix-assisted laser desorption ionization time-of-flight mass spectrometry (UV-..
  6. Andreev Y, Kozlov S, Vassilevski A, Grishin E. Cyanogen bromide cleavage of proteins in salt and buffer solutions. Anal Biochem. 2010;407:144-6 pubmed publisher
    ..To perform cyanogen bromide (CNBr) cleavage of fusion proteins, the majority of researchers first desalted and vacuum-dried samples and ..
  7. Vanelslander B, Paul C, Grueneberg J, Prince E, Gillard J, Sabbe K, et al. Daily bursts of biogenic cyanogen bromide (BrCN) control biofilm formation around a marine benthic diatom. Proc Natl Acad Sci U S A. 2012;109:2412-7 pubmed publisher
    ..exudes a diverse mixture of volatile iodinated and brominated metabolites including the natural product cyanogen bromide (BrCN), which exhibits pronounced allelopathic activity...
  8. DeLange R, Hooper J, Smith E. Histone 3. 3. Sequence studies on the cyanogen bromide peptides; complete amino acid sequence of calf thymus histone 3. J Biol Chem. 1973;248:3261-74 pubmed
  9. Machleidt W, Thiele U, Laber B, Assfalg Machleidt I, Esterl A, Wiegand G, et al. Mechanism of inhibition of papain by chicken egg white cystatin. Inhibition constants of N-terminally truncated forms and cyanogen bromide fragments of the inhibitor. FEBS Lett. 1989;243:234-8 pubmed
    N-terminally truncated forms of chicken egg white cystatin and its cyanogen bromide fragments were isolated and assayed for inhibition of papain...

More Information

Publications113 found, 100 shown here

  1. Prinz T, Müller J, Kuhn K, Schafer J, Thompson A, Schwarz J, et al. Characterization of low abundant membrane proteins using the protein sequence tag technology. J Proteome Res. 2004;3:1073-81 pubmed
    ..proteins, which relies on a "sampling" strategy by isolating N-terminal protein sequence tags from cyanogen bromide cleaved proteins. The identification of these N-terminal PST peptides is based on LC-MS/MS...
  2. Welply J, Fowler A, Zabin I. beta-Galactosidase alpha-complementation. Overlapping sequences. J Biol Chem. 1981;256:6804-10 pubmed
    ..Several antipeptide antibodies that react with these two proteins do not react with beta-galactosidase. M112 protein, like M15 protein, can be activated by anti-beta-galactosidase but to a much higher level. ..
  3. Tang H, Speicher D. Determination of disulfide-bond linkages in proteins. Curr Protoc Protein Sci. 2004;Chapter 11:Unit 11.11 pubmed publisher
    ..A partial reduction and alkylation strategy for mapping disulfide linkages in peptides with multiple disulfide bonds is also presented. ..
  4. Lin A, McNally J, Wool I. The primary structure of rat liver ribosomal protein L39. J Biol Chem. 1984;259:487-90 pubmed
    ..Two peptides, CNI (28 residues) and CNII (22 residues), were produced by cleavage of protein L39 with cyanogen bromide and the sequence of CNII was determined by automated Edman degradation...
  5. Maloy W, Coligan J. Primary structure of the H-2Db alloantigen. II. Additional amino acid sequence information, localization of a third site of glycosylation and evidence for K and D region specific sequences. Immunogenetics. 1982;16:11-22 pubmed
    ..Comparison of this sequence with other available protein sequence information for murine class I molecules has revealed protein sequences that may be unique to either K or D region molecules. ..
  6. van Montfort B, Doeven M, Canas B, Veenhoff L, Poolman B, Robillard G. Combined in-gel tryptic digestion and CNBr cleavage for the generation of peptide maps of an integral membrane protein with MALDI-TOF mass spectrometry. Biochim Biophys Acta. 2002;1555:111-5 pubmed
    ..The fragments formed are predominantly below m/z 2500, which allows accurate mass measurement. ..
  7. Ho J, White J, Grisshammer R, Hess S. Analysis of a G protein-coupled receptor for neurotensin by liquid chromatography-electrospray ionization-mass spectrometry. Anal Biochem. 2008;376:13-24 pubmed publisher
    ..In a separate experiment, NTS1 was digested with a combination of cyanogen bromide and trypsin and/or chymotrypsin...
  8. Iwaki M, Kagamiyama H, Nozaki M. The primary structure of the beta-subunit of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa. Arch Biochem Biophys. 1981;210:210-23 pubmed
  9. Xue J, Kalafatis M, Silveira J, Kung C, Mann K. Determination of the disulfide bridges in factor Va heavy chain. Biochemistry. 1994;33:13109-16 pubmed
    ..using iodo[14C]acetamide-labeled factor Va heavy chain digested with trypsin, plasmin, V-8 protease, and cyanogen bromide. Following HPLC separation of the resulting peptides, free cysteines were identified by the incorporation of ..
  10. Zimmermann L, Schwaller B. Monoclonal antibodies recognizing epitopes of calretinins: dependence on Ca2+-binding status and differences in antigen accessibility in colon cancer cells. Cell Calcium. 2002;31:13-25 pubmed
    ..coli in order to characterize the epitopes recognized by the three antibodies. Additionally, tryptic and cyanogen bromide fragments were produced to further narrow down the sequences recognized by the three antibodies...
  11. Jacksén J, Redeby T, Emmer A. Capillary electrophoretic separation and fractionation of hydrophobic peptides onto a pre-structured matrix assisted laser desorption/ ionization target for mass spectrometric analysis. J Sep Sci. 2006;29:288-95 pubmed
    ..CE-MALDI-MS analysis of nine highly hydrophobic peptides from cyanogen bromide-digested bacteriorhodopsin is demonstrated.
  12. Nagore L, Mitra S, Jiang D, Jiang S, Zhou Y, Loranc M, et al. Cyanogen bromide-activated coupling: DNA catalytic chromatography purification of EcoRI endonuclease. Nat Protoc. 2006;1:2909-15 pubmed
    ..of EcoRI endonuclease using a sequence-specific DNA column (containing the GAATTC motif coupled to cyanogen bromide-activated Sepharose 4B) binds EcoRI in the absence of Mg2+ and elutes when Mg2+ is applied in a highly ..
  13. Kimball E, Nathenson S, Coligan J. Amino acid sequence of residues 1-98 of the K-2Kb murine major histocompatibility alloantigen: comparison with H-2Kb and H-2db reveals extensive localized differences. Biochemistry. 1981;20:3301-8 pubmed
  14. Rao U, Denslow N, Block E. Hypoxia induces the synthesis of tropomyosin in cultured porcine pulmonary artery endothelial cells. Am J Physiol. 1994;267:L271-81 pubmed
    ..The partial amino acid sequence of the 35-kDa protein obtained from cyanogen bromide cleavage of the molecule was Asp-Ala-Ile-Lys-Lys-Lys-Met-Gln-Met-Leu-Lys-Leu-Asp-Lys-Glu...
  15. Stabeli R, Amui S, Sant Ana C, Pires M, Nomizo A, Monteiro M, et al. Bothrops moojeni myotoxin-II, a Lys49-phospholipase A2 homologue: an example of function versatility of snake venom proteins. Comp Biochem Physiol C Toxicol Pharmacol. 2006;142:371-81 pubmed
    ..and antiparasitic effects); (ii) effects of structural modifications by 4-bromophenacyl bromide (BPB), cyanogen bromide (CNBr), acetic anhydride and 2-nitrobenzenesulphonyl fluoride (NBSF); (iii) enzymatic characterization: ..
  16. Potetinova Z, Tantry S, Cohen L, Caroccia K, Arshava B, Becker J, et al. Large multiple transmembrane domain fragments of a G protein-coupled receptor: biosynthesis, purification, and biophysical studies. Biopolymers. 2012;98:485-500 pubmed publisher
    ..protein using a modified pMMHa vector in L-arabinose-induced Escherichia coli BL21-AI, and cleaved with cyanogen bromide. 4TMC and 5TMC were obtained by direct expression using a pET21a vector in IPTG-induced E...
  17. Giudici C, Viola M, Tira M, Forlino A, Tenni R. Molecular stability of chemically modified collagen triple helices. FEBS Lett. 2003;547:170-6 pubmed
    ..These properties can probably be explained by the fact that chemically modified groups maintain the ability to form hydrogen bonds. ..
  18. KLINE K, Wu C. MudPIT analysis: application to human heart tissue. Methods Mol Biol. 2009;528:281-93 pubmed publisher
    ..Methods discussed will include tissue homogenization, sample preparation, MudPIT, data analysis, and an application for the analysis of unfractionated total tissue homogenate from human heart. ..
  19. Yang C, Horwitz S. Taxol mediates serine phosphorylation of the 66-kDa Shc isoform. Cancer Res. 2000;60:5171-8 pubmed
    ..b>Cyanogen bromide digestion of p66shc produced a phosphorylated fragment with an apparent molecular weight of approximately 7...
  20. Monzani E, Curto M, Galliano M, Minchiotti L, Aime S, Baroni S, et al. Binding and relaxometric properties of heme complexes with cyanogen bromide fragments of human serum albumin. Biophys J. 2002;83:2248-58 pubmed
    The spectroscopic and reactivity properties of hemin complexes formed with cyanogen bromide fragments B (residues 1-123), C (124-298), A (299-585), and D (1-298) of human serum albumin (HSA) have been investigated...
  21. Lisenbee C, Dong M, Miller L. Paired cysteine mutagenesis to establish the pattern of disulfide bonds in the functional intact secretin receptor. J Biol Chem. 2005;280:12330-8 pubmed
    ..Furthermore, cyanogen bromide cleaved a series of wild type and mutant secretin receptors, yielding patterns that agreed with our paired ..
  22. Christie D, Gagnon J. Amino acid sequence of the Bb fragment from complement Factor B. Sequence of the major cyanogen bromide-cleavage peptide (CB-II) and completion of the sequence of the Bb fragment. Biochem J. 1983;209:61-70 pubmed
    ..Factor B has a catalytic chain (fragment Bb) with a molecular weight twice that of proteinases previously described, suggesting that it is a novel type of serine proteinase, probably with a different activation mechanism. ..
  23. Sigalov A, Stern L. Enzymatic repair of oxidative damage to human apolipoprotein A-I. FEBS Lett. 1998;433:196-200 pubmed
  24. Qazi O, Hitchen P, Tissot B, Panico M, Morris H, Dell A, et al. Mass spectrometric analysis of the S-layer proteins from Clostridium difficile demonstrates the absence of glycosylation. J Mass Spectrom. 2009;44:368-74 pubmed publisher
    ..Furthermore, analysis of Cyanogen bromide (CNBr) and tryptic peptides displayed no evidence of post-translational modification...
  25. Ozols J, Carr S, Strittmatter P. Identification of the NH2-terminal blocking group of NADH-cytochrome b5 reductase as myristic acid and the complete amino acid sequence of the membrane-binding domain. J Biol Chem. 1984;259:13349-54 pubmed
  26. Akeroyd R, Moonen P, Westerman J, Puyk W, Wirtz K. The complete primary structure of the phosphatidylcholine-transfer protein from bovine liver. Isolation and characterization of the cyanogen bromide peptides. Eur J Biochem. 1981;114:385-91 pubmed
  27. Oxvig C, Sand O, Kristensen T, Gleich G, Sottrup Jensen L. Circulating human pregnancy-associated plasma protein-A is disulfide-bridged to the proform of eosinophil major basic protein. J Biol Chem. 1993;268:12243-6 pubmed
    ..In addition, it is shown that commercial polyclonal anti-PAPP-A is polyspecific, also reacting with MBP. ..
  28. Tenni R, Viola M, Welser F, Sini P, Giudici C, Rossi A, et al. Interaction of decorin with CNBr peptides from collagens I and II. Evidence for multiple binding sites and essential lysyl residues in collagen. Eur J Biochem. 2002;269:1428-37 pubmed
    ..We conclude that decorin makes contacts with multiple sites in type I collagen and probably also in type II collagen and that some collagen Lys/Hyl residues are essential for the binding. ..
  29. Aitken A, Klee C, Cohen P. The structure of the B subunit of calcineurin. Eur J Biochem. 1984;139:663-71 pubmed
  30. Levy M, Ellington A. Peptide-templated nucleic acid ligation. J Mol Evol. 2003;56:607-15 pubmed
    ..b>Cyanogen bromide activation was transient relative to oligonucleotides that terminated with a 5'-iodine and a 3'..
  31. Dwivedi Y, Laurent M, Sarvepalli S, Schmid T, Wimmer M. Albumin Protein Cleavage Affects the Wear and Friction of Ultra-High Molecular Weight Polyethylene. Lubricants. 2017;5: pubmed publisher
    ..Cleavage of the bovine albumin into five fragments was performed by digestion with cyanogen bromide. In pin-on-flat (POF) wear tests of polyethylene pins made of Ticona GUR® 1020/1050 against ..
  32. Morgan T, Ray N, Parry D. A solid-phase route to N-cyanoamides. Org Lett. 2002;4:597-8 pubmed
    ..The attachment of a secondary amine to solid support is accomplished using Merrifield resin. After functionalization, cleavage is readily achieved with cyanogen bromide to afford the desired cyanamide.
  33. Naranda T, Kaufman R, Li J, Wong K, Boge A, Hallén D, et al. Activation of erythropoietin receptor through a novel extracellular binding site. Endocrinology. 2002;143:2293-302 pubmed
    ..The activity is comparable to that of EPO. Recognition of such receptor sites represents a new and important concept in receptor function. ..
  34. Chen W, Liu W, Yang M, Hwang M, Tsao J, Mao S. A novel conformation-dependent monoclonal antibody specific to the native structure of beta-lactoglobulin and its application. J Dairy Sci. 2006;89:912-21 pubmed
    ..From a technological standpoint, the mAb prepared in this study is relevant to the design and operation of appropriate processes for thermal sanitation of milk and of other dairy products. ..
  35. Adel Patient K, Nutten S, Bernard H, Fritsché R, Ah Leung S, Meziti N, et al. Immunomodulatory potential of partially hydrolyzed ?-lactoglobulin and large synthetic peptides. J Agric Food Chem. 2012;60:10858-66 pubmed publisher
    ..and chemically denatured BLG (lacking S-S bridges, dBLG), products resulting from their hydrolysis using cyanogen bromide (CNBr) and some synthetic peptides, were produced and precisely characterized...
  36. Haggarty N, Dunbar B, Fothergill L. The complete amino acid sequence of human erythrocyte diphosphoglycerate mutase. EMBO J. 1983;2:1213-20 pubmed
    ..The sequence was deduced from the four cyanogen bromide fragments, and from the peptides derived from these fragments after digestion with a number of proteolytic ..
  37. Sabater M, Heilmann S, Frøkiaer H, Biedermann K, Emborg C. The use of antibodies for characterization and quantification of a recombinant protein. Ann N Y Acad Sci. 1996;782:462-77 pubmed
    ..This study allowed us to develop an ELISA method for the quantification of proteinase A in culture supernatants as well as the evaluation of monoclonal antibodies for their use in immunoaffinity chromatography. ..
  38. Brennan S, George P. Three truncated forms of serum albumin associated with pancreatic pseudocyst. Biochim Biophys Acta. 2000;1481:337-43 pubmed
    ..This suggested that the des-Leu(585) form might be present at low levels in the plasma of normal individuals and CNBr mapping confirmed that it constituted 4-15% of the albumin from normal plasma. ..
  39. Bornstein P. Covalent cross-links in collagen: a personal account of their discovery. Matrix Biol. 2003;22:385-91 pubmed
  40. Mahoney W, Hogg R, Hermodson M. The amino acid sequence of the D-galactose-binding protein from Escherichia coli B/r. J Biol Chem. 1981;256:4350-6 pubmed
    ..galactose-binding protein was determined by the automated sequencing of fragments produced by cleavage with cyanogen bromide, o-iodosobenzoic acid, limited trypsin digestion, mild acid hydrolysis, and Staphylococcus aureus strain V8 ..
  41. Zahran S, Pan J, Liu P, Hwang P. Combining a PagP fusion protein system with nickel ion-catalyzed cleavage to produce intrinsically disordered proteins in E. coli. Protein Expr Purif. 2015;116:133-8 pubmed publisher
    ..Moreover, nickel-catalyzed cleavage is more specific than the most commonly used agent, cyanogen bromide, which cleaves C-terminal to methionine residues...
  42. FONTANILLA D, Hajipour A, Pal A, Chu U, Arbabian M, Ruoho A. Probing the steroid binding domain-like I (SBDLI) of the sigma-1 receptor binding site using N-substituted photoaffinity labels. Biochemistry. 2008;47:7205-17 pubmed publisher
    ..51, 283-292]. Cleavage of the photolabeled sigma-1 receptor using Endo Lys C and cyanogen bromide (CNBr) revealed that the [ (125)I]-N-IACoc label was located primarily in the N-terminus and SBDLI-containing ..
  43. Vogel H, Gerlach C, Richert C. Reactions of buffers in cyanogen bromide-induced ligations. Nucleosides Nucleotides Nucleic Acids. 2013;32:17-27 pubmed publisher
    ..between a phosphate-terminated oligonucleotide and an unphosphorylated reaction partner may be induced by cyanogen bromide (BrCN)...
  44. Slootweg J, Liskamp R, Rijkers D. Scalable purification of the lantibiotic nisin and isolation of chemical/enzymatic cleavage fragments suitable for semi-synthesis. J Pept Sci. 2013;19:692-9 pubmed publisher
  45. Clark H, Hagedorn T, Landino L. Hypothiocyanous acid oxidation of tubulin cysteines inhibits microtubule polymerization. Arch Biochem Biophys. 2014;541:67-73 pubmed publisher
    ..Due to its instability and buffer- and pH-dependent reactivity, hypothiocyanous acid studies require careful consideration of reaction conditions. ..
  46. Chen R, Kramer C, Schmidmayr W, Chen Schmeisser U, Henning U. Primary structure of major outer-membrane protein I (ompF protein, porin) of Escherichia coli B/r. Biochem J. 1982;203:33-43 pubmed
    ..To aid further studies on this class of transmembrane proteins, the isolation of most of the constituent peptides is documented. ..
  47. Fricke T, Mart R, Watkins C, Wiltshire M, Errington R, Smith P, et al. Chemical synthesis of cell-permeable apoptotic peptides from in vivo produced proteins. Bioconjug Chem. 2011;22:1763-7 pubmed publisher
    ..Here, we present a method for the facile modification of peptides generated in bacterial hosts after cyanogen bromide cleavage at C-terminal methionines...
  48. Bisaccia F, Zara V, Capobianco L, Iacobazzi V, Mazzeo M, Palmieri F. The formation of a disulfide cross-link between the two subunits demonstrates the dimeric structure of the mitochondrial oxoglutarate carrier. Biochim Biophys Acta. 1996;1292:281-88 pubmed
  49. Deyl Z, Miksik I, Zicha J. Multicomponent analysis by off-line combination of synchronous fluorescence spectroscopy and capillary electrophoresis of collagen glycation adducts. J Chromatogr A. 1999;836:161-71 pubmed
  50. Samyn B, Sergeant K, Van Beeumen J. A method for C-terminal sequence analysis in the proteomic era (proteins cleaved with cyanogen bromide). Nat Protoc. 2006;1:318-23 pubmed
    ..A peptide mixture is generated by cleavage of the protein with cyanogen bromide and is incubated with carboxypeptidase Y...
  51. Ceciliani F, Faotto L, Negri A, Colombo I, Berra B, Bartorelli A, et al. The primary structure of UK114 tumor antigen. FEBS Lett. 1996;393:147-50 pubmed
  52. Jurado L, Mosley J, Jarrett H. Cyanogen bromide activation and coupling of ligands to diol-containing silica for high-performance affinity chromatography optimization of conditions. J Chromatogr A. 2002;971:95-104 pubmed
    ..Activation of the silane-derived hydroxyls with cyanogen bromide and triethylamine was optimized and demonstrated to efficiently couple several amino ligands (tryptophan, 6-..
  53. Tanaka Y, Fukumori Y, Yamanaka T. The complete amino acid sequence of Nitrobacter agilis cytochrome c-550. Biochim Biophys Acta. 1982;707:14-20 pubmed
    ..In particular, its primary structure was very similar to that of Rhodopseudomonas viridis cytochrome c2. Some of its properties were compared with those of other cytochromes c on the basis of the primary structure...
  54. Berchtold M, Heizmann C, Wilson K. Primary structure of parvalbumin from rat skeletal muscle. Eur J Biochem. 1982;127:381-9 pubmed
    ..A prediction of the secondary structure has been made and found to be very similar for the rat and rabbit proteins with the exception of the sequence region 72-78, located between the Ca2+, Mg2+-binding CD and EF domains...
  55. Jones M, Petersen T, Nielsen K, Magnusson S, Sottrup Jensen L, Gausing K, et al. The complete amino-acid sequence of elongation factor Tu from Escherichia coli. Eur J Biochem. 1980;108:507-26 pubmed
    ..Their alignment was established with overlapping peptides obtained by chemical cleavage with cyanogen bromide and subsequent enzymic subdigestion with Staphylococcus aureus protease, chymotrypsin, elastase and ..
  56. Laursen R, L Italien J, Nagarkatti S, Miller D. The amino acid sequence of elongation factor Tu of Escherichia coli. The complete sequence. J Biol Chem. 1981;256:8102-9 pubmed
    ..acid sequence of elongation factor Tu of Escherichia coli has been established by sequencing overlapping cyanogen bromide and tryptic peptides. Sequence analysis of peptides was done primarily by solid-phase Edman degradation...
  57. Dell Angelica E, Ermacora M, Santome J. Purification and partial characterization of a fatty acid-binding protein from the yeast, Yarrowia lipolytica. Biochem Mol Biol Int. 1996;39:439-45 pubmed
    ..The isolated protein exists as a monomer of 15 kDa, is basic and has a blocked N-terminus. Internal amino acid sequencing suggests that this protein may belong to a novel class of fatty acid-binding proteins...
  58. Verma J, Sridhara S, Singh B, Pasha S, Gangal S, Arora N. Fusarium solani major allergen peptide IV-1 binds IgE but does not release histamine. Clin Exp Allergy. 2001;31:920-7 pubmed
    ..Fusarium solani (FS) is an important allergen source afflicting 4% of the nasobronchial allergy patients. Fus s I3596*, a 65 kDa major glycoprotein allergen of FS reacts with 95% fungus sensitive patients...
  59. Whitelegge J, Ahn V, Norris A, Sung H, Waring A, Stevens R, et al. Characterization of a recombinant molecule covalently indistinguishable from human cerebroside-sulfate activator protein (CSAct or Saposin B). Cell Mol Biol (Noisy-le-grand). 2003;49:799-807 pubmed
    ..It is concluded that while glycosylation is unnecessary for in vitro and ex vivo activity of CSAct, modification of the native N21 is necessary to prevent loss of ex vivo activity, possibly via protection from degradation...
  60. Roher A, Kokjohn T, Esh C, Weiss N, Childress J, Kalback W, et al. The human amyloid-beta precursor protein770 mutation V717F generates peptides longer than amyloid-beta-(40-42) and flocculent amyloid aggregates. J Biol Chem. 2004;279:5829-36 pubmed
    ..of ultracentrifugation, size-exclusion, and reverse-phase high performance liquid chromatography, tryptic and cyanogen bromide hydrolysis, amino acid analysis, and matrix-assisted laser desorption ionization and surface-enhanced laser ..
  61. Lewis W, Smillie L. The amino acid sequence of rabbit cardiac tropomyosin. J Biol Chem. 1980;255:6854-9 pubmed
    ..acid sequence of rabbit cardiac tropomyosin has been investigated by the isolation of peptides derives from cyanogen bromide and proteolytic degradations...
  62. Somani A, Bignon J, Mills G, Siminovitch K, Branch D. Src kinase activity is regulated by the SHP-1 protein-tyrosine phosphatase. J Biol Chem. 1997;272:21113-9 pubmed
    ..These observations, together with the finding of reduced Src activity in HEY cells expressing a dominant negative form of SHP-1, provide compelling evidence that SHP-1 functions include the positive regulation of Src activation...
  63. Sarafova A, Choi H, Forlino A, Gajko A, Cabral W, Tosi L, et al. Three novel type I collagen mutations in osteogenesis imperfecta type IV probands are associated with discrepancies between electrophoretic migration of osteoblast and fibroblast collagen. Hum Mutat. 1998;11:395-403 pubmed
    ..b>Cyanogen bromide digestion localized these cross-links to CB8 and 3, respectively...
  64. Quach T, Li N, Richards D, Zheng J, Keller B, Li L. Development and applications of in-gel CNBr/tryptic digestion combined with mass spectrometry for the analysis of membrane proteins. J Proteome Res. 2003;2:543-52 pubmed
    ..The results are presented from a study of the analysis of bacteriorhodopsin, nitrate reductase 1 gamma chain, and a complex protein mixture extracted from the endoplasmic recticulum membrane of mouse liver...
  65. Lee J, Kwon J, Baek M. A combination method of chemical with enzyme reactions for identification of membrane proteins. Biochim Biophys Acta. 2011;1814:397-404 pubmed publisher
    ..In particular, many membrane proteins identified in this study were detected by a new combination, but not by a traditional trypsin digestion method...
  66. Ovchinnikov I, Alakhov I, Bundulis I, Bundule M, Vinokurov L. [Primary structure of the elongation factor G from Escherichia coli. IX. Structure of peptides generated by cyanogen bromide cleavage of the G-factor isolated on thiol-activated sepharose and of the products of the G-factor cleavage at Asp-Pro bonds. Bioorg Khim. 1983;9:343-57 pubmed
    ..The amino acid sequence of cysteine- and cystine-containing peptides resulting from cleavage of the G-factor by cyanogen bromide has been determined...
  67. Takio K, Towatari T, Katunuma N, Teller D, Titani K. Homology of amino acid sequences of rat liver cathepsins B and H with that of papain. Proc Natl Acad Sci U S A. 1983;80:3666-70 pubmed
    ..The results not only reveal the first structural features of mammalian thiol endopeptidases but also provide insight into the evolutionary relationships among plant and mammalian thiol proteases...
  68. Snow P, Terhorst C. The T8 antigen is a multimeric complex of two distinct subunits on human thymocytes but consists of homomultimeric forms on peripheral blood T lymphocytes. J Biol Chem. 1983;258:14675-81 pubmed
    ..We suggest that the switch from heteromultimers to homomultimers may occur concomitantly with the last step in T lymphocyte maturation...
  69. Tratschin J, Wirz B, Frank G, Zuber H. Structure and function of L-lactate dehydrogenases from thermophilic and mesophilic bacteria. II) The primary structure of thermophilic lactate dehydrogenase from Bacillus stearothermophilus. Cyanogen bromide fragments and partial sequence. Hoppe Seylers Z Physiol Chem. 1983;364:879-92 pubmed
    The polypeptide chain of thermophilic lactate dehydrogenase from Bacillus stearothermophilus was split with cyanogen bromide. The 6 cyanogen bromide fragments were then separated and isolated by gel filtration (Bio-Gel P 10, Sephadex G-75)..
  70. Wang Q, Orrison B, Marini J. Two additional cases of osteogenesis imperfecta with substitutions for glycine in the alpha 2(I) collagen chain. A regional model relating mutation location with phenotype. J Biol Chem. 1993;268:25162-7 pubmed
    ..b>Cyanogen bromide peptides of the overmodified alpha 1(I) chains revealed delayed migration of all peptides except CB6...
  71. Ablonczy Z, Kono M, Crouch R, Knapp D. Mass spectrometric analysis of integral membrane proteins at the subnanomolar level: application to recombinant photopigments. Anal Chem. 2001;73:4774-9 pubmed
    ..The methodology presented here will enable mass spectrometric analysis of subnanomolar levels of photopigments or other integral membrane proteins either from their native membranes or as products of expression systems...
  72. Pham V, Wade J, Purdue B, Sexton P. Spatial proximity between a photolabile residue in position 19 of salmon calcitonin and the amino terminus of the human calcitonin receptor. J Biol Chem. 2004;279:6720-9 pubmed
    ..b>Cyanogen bromide cleavage together with endoproteinase Asp-N digestion indicated that binding was predominantly to the region ..
  73. Werner L, Machara A, Adams D, Cox D, Hudlicky T. Synthesis of buprenorphine from oripavine via N-demethylation of oripavine quaternary salts. J Org Chem. 2011;76:4628-34 pubmed publisher
    ..The new synthesis avoids toxic reagents used previously, is shorter, and proceeds in comparable yields. Experimental and spectral data are provided for all new compounds...
  74. Carter P, Dunbar B, Fothergill J. The serine proteinase chain of human complement component C1s. Cyanogen bromide cleavage and N-terminal sequences of the fragments. Biochem J. 1983;215:565-71 pubmed
    ..Somewhat surprisingly, when the C1s sequence is compared with that of complement subcomponent C1r, the percentage difference (59%) is approximately the same as that found between the other mammalian serine proteinases (56-71%)...
  75. Takahashi N, Takahashi Y, Putnam F. Structure of human hemopexin: O-glycosyl and N-glycosyl sites and unusual clustering of tryptophan residues. Proc Natl Acad Sci U S A. 1984;81:2021-5 pubmed
  76. Stenflo J, Fernlund P. Amino acid sequence of the heavy chain of bovine protein C. J Biol Chem. 1982;257:12180-90 pubmed
    ..The sequence is homologous with those of the other serine proteases. On activation, an NH2-terminal tetradecapeptide is removed...
  77. Bandi H, Ferrari S, Krieg J, Meyer H, Thomas G. Identification of 40 S ribosomal protein S6 phosphorylation sites in Swiss mouse 3T3 fibroblasts stimulated with serum. J Biol Chem. 1993;268:4530-3 pubmed
    ..in 40 S ribosomal protein S6 derived from serum-stimulated Swiss mouse 3T3 cells are found within a small cyanogen bromide (CNBr) peptide derived from the carboxyl terminus, Lys218-Lys249...
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