monoclonal antibodies

..Use the same immunogens to isolate human monoclonal antibodies from a synthetic phage library expressing 1011 different single chain human antibodies (scFv) (..

..C3A. VERTEBRATE ANIMALS (Resume): No concerns. The applicants plan to generate mouse monoclonal antibodies against the same VIP and TMP synthetic polypeptides used successfully already in rabbits...

..anthracis infection. Modernization of viral safety approaches and technology for monoclonal antibodies. (Kurt Brorson) Murine hybridoma cells used in the production of monoclonal antibodies (mAbs) produce ..

..titer anti-pertussis immunoglobulin in the treatment of pertussis in infants, we sought to develop human monoclonal antibodies and single-chain antibodies against pertussis toxin for use in pertussis therapy...

..A mixture of just three human monoclonal antibodies to envelope is capable of protecting monkeys against challenge...

..We have focused on two monoclonal antibodies to AA transporters that inhibit virus replication, but with slightly different potencies and effects...

..In other studies, we have recovered human monoclonal antibodies that react with HCV...

..The specificity of detection was verified by the staining of serial sections with two different monoclonal antibodies directed against two non-overlapping epitopes on uPARAP and by the use of isotype-matched non-immune ..

..1) The search of protein surface IgE binding epitopes will be conducted using monoclonal antibodies against hyaluronidase, which compete with IgE antibodies...

..These receptor preparations were used to generate monoclonal antibodies and we are exploring the effect of receptor?antibody fragment co-crystallization on crystal quality...

..deficient in the non-shared receptors (p35 for IL-12 and p19 for IL-23), and the respective neutralizing monoclonal antibodies. Data indicate that IL-23, not IL-12, is an obligatory cytokine for induction of EAU...

..New results: We characterized the epitopes of two monoclonal antibodies, including that of the first IgM to be analyzed in this way (i.e. cryo-EM and modelling)...

..Site-specific monoclonal antibodies to various titin sequence domains have been prepared and applied in immunoelectron microscopy to delineate ..

..Three of the bands could either be precipitated by anti-CXCR4 monoclonal antibodies (101 and 47 kDa) or co-precipitated with CD4 (62 kDa)...

Summary: Murine hybridoma cells used in the production of monoclonal antibodies (mAbs) produce endogenous type C retrovirus particles...

..b>Monoclonal antibodies to these sites can neutralize a broad range of HIV-1 isolates and have protected monkeys against viral ..

..TNF signaling on FoxP3 expression at the mRNA level as well as the protein level using recently released monoclonal antibodies to FoxP3 and western blotting and eventually flow cytometry when monoclonal antibodies appropriate for ..

..changes in the glycoprotein Gp IIb/IIIa , induced by activation of platelets by agonists or by binding of monoclonal antibodies to this glycoprotein, can lead to fibrinogen binding to platelets and to platelet aggregation...

..We identified a quadruple (4x) combination of human neutralizing monoclonal antibodies (nmAbs) that, although raised against clade B, was broadly reactive across clades, including clade C...

..We identified a quadruple (4x) combination of human neutralizing monoclonal antibodies (nmAbs) that, although raised against clade B, was broadly reactive across clades, including clade C...

..Rabbit Immune Repertoires for Generation and Humanization of Therapeutic Monoclonal Antibodies The rabbit immune repertoire has long been a rich source of diagnostic polyclonal antibodies...

..RRPs offer many advantages over monoclonal antibodies, including rapid turnaround and economical expression...

..titer anti-pertussis immunoglobulin in the treatment of pertussis in infants, we sought to develop human monoclonal antibodies and single-chain antibodies against pertussis toxin for use in pertussis therapy...

..b>Monoclonal antibodies distinguish antigenic differences between "Western" and "Eastern" strains of R...

..Along with LEC/NHS76, this new antibody/drug conjugate should be applicable to use in humans and may provide clinicians with a vital weapon in our relentless effort to treat cancer...

..properties of human IFN-a2c, IFN-a21a, IFN-a21a/a2c hybrids and their mutants using a panel of 26 anti-IFN monoclonal antibodies (anti-IFN-a1, a2 and a 8/1/8) and have found IFN-a2c and IFN-a21a to be antigenically distinct...

..Extensive use will be made to high affinity monoclonal antibodies (mAbs) specific for each collagen type for which the location of different epitopes along the collagen ..

We have been evaluating the effect of immune modulation, accomplished through administration of monoclonal antibodies or genetic manipulation, on allergic responses and susceptibility to infection or tumors...

..elicitation of a lymphoma specific T-cell response, such as GM-CSF, CpG oligonucleotides, and anti-CTLA-4 monoclonal antibodies, would be of potential clinical benefit...

..We have raised monoclonal antibodies to synthetic peptides corresponding to sequences in the ECD of the receptor...

..designed to reduce binding to the FcR and minimize toxicities associated with conventional anti-CD3 monoclonal antibodies. This study is based on preliminary data derived from a Phase I dose escalation trial of anti-CD3 in ..

..Specific Aim 1 is to clone cell lines producing murine and rhesus macaque monoclonal antibodies (MAb) specific for LASV glycoproteins and nucleoprotein...

..Results: using surface plasmon resonance (Biacore), a kinetic-affinity map of a panel of monoclonal antibodies (mAbs) against HBV nucleocapsid proteins revealed a range of binding affinities...

..Protein-protein interactions are studied utilizing monoclonal antibodies (mAbs) specific for hen egg white lysozyme (HEL), a protein which has long served as a prototype for ..

..model of human anti-GBM disease that has been developed in our laboratory, I propose to generate human monoclonal antibodies (mAb), directed towards 2 putatively pathogenically relevant epitopes on Antibodies against the GBM...

..and Kearney will generate hybridomas from those mice that express neutralizing MPER antibodies and obtain monoclonal antibodies for further study...

..Second, one of the few broadly neutralizing HIV monoclonal antibodies, 2G12, recognizes a conformational epitope of high mannose type glycans on gp120...

..This prominent and abundant protein is expressed on the surface of inner ear supporting cells (IESC). Monoclonal antibodies against CTL2 have been shown to induce hearing loss in an experimental guinea pig model...

Chimpanzee monoclonal antibodies that efficiently neutralize dengue viruses, Japanese encephalitis virus, or tick-borne encephalitis virus have been identified from panels of Fab antibody fragments recovered by repertoire cloning...

..a standardized procedure involving the use of immunological tolerance for producing highly-specific monoclonal antibodies (mAbs) reactive with unique epitopes of Bacillus anthracis (the ethological agent of anthrax) but not with ..

b>Monoclonal antibodies selectively bind tumor cell differentiation antigens in vitro and in vivo...

..These antibodies could also be used to develop products to treat HIV infection when used in conjunction with anti-retroviral drugs. Public Health Relevance: This Public Health Relevance is not available...

..Long-circulating liposomes can also be made targeted by attaching specific ligands, such as monoclonal antibodies to their surface...

..for use in case of a bioterrorist attack with Bacillus anthracis; however, cell lines making anti-PA monoclonal antibodies (mAbs) would provide a ready source for such reagents...

..Research Design and Methods; Aim 1: Produce monoclonal antibodies to B. anthracis spores. The studies will use Sterne strain (pXO 1+, pXO2-) B...

DESCRIPTION (provided by applicant): During the previous funding cycle we identified five monoclonal antibodies (MAbs) against the P1 surface adhesin of Streptococcus mutans that redirect the humoral immune response in mice in terms of ..

..Specific Aim 1 is to obtain and characterize panels of monoclonal antibodies (mAbs) that react with HA and NA of influenza H3N2 isolates from 2002 through the 5 year grant...

..L.C., and generate rat monoclonal antibodies to epitopes specific to the Amygdala of mice...

DESCRIPTION (provided by applicant): During the previous funding cycle we identified five monoclonal antibodies (MAbs) against the P1 surface adhesin of Streptococcus mutans that redirect the humoral immune response in mice in terms of ..

..EAM disease process can be blocked by: (1) cobra venom factor (CVF), which depletes complement; or by (2) monoclonal antibodies to the complement receptors involved with the innate immune system; or by (3) antibodies to IL-4 or by (4) ..

..The Specific Aims of the proposal are to 1) Map binding epitopes of a panel of monoclonal antibodies that recognize distinct regions of the FIV envelope glycoprotein...

..gp120, and has been shown to contain conserved neutralizing epitopes such as those recognized by the human monoclonal antibodies 2F5, 4E10, and Z13...

..polymers, liposomes, and micelles should enhance contrast accumulation in the targets; however, diagnostic monoclonal antibodies are usually tumor type-specific and unable to react with a broad variety of tumors...

..Long-circulating liposomes can also be made targeted by attaching specific ligands, such as monoclonal antibodies to their surface...

..The most promising of these will be evaluated in animal models and hopefully in human clinical trials...

..In order to study cathepsin W, we expressed human procathepsin W in E. coli and made a series of monoclonal antibodies against it...

..Radiolabeled anti-CD45 monoclonal antibodies (Ab) have been shown to improve outcomes for AML in the setting of SCT, but toxicity remains high and cure ..

..Polyclonal and monoclonal antibodies are the only widely available affinity reagents for histone tails, but they have fundamental limitations ..

..project: Project 1: to determine whether there is variability in the electrophysiologic signature of monoclonal antibodies to the NMDAR corresponding with functional variability; Project 2: to determine whether maternal ..

DESCRIPTION (provided by applicant): Monoclonal antibodies (MAb) are potential new therapeutics for many brain diseases, including Alzheimer's disease (AD), Parkinson's disease, mad cow disease, West Nile encephalitis, neuro-AIDS, brain ..

A panel of six monoclonal antibodies (mAbs) recognizing antigenic determinants on canine immunoglobulin (Ig) heavy or light chains was produced and characterized...

..As a result, we conclude that peptide H98 has potential for being developed as a HER-2 vaccine for biotherapy of cancer with HER-2 overexpression...

..This report describes an example of disparity between antigenicity and cross-reactive immunogenicity, complicating the selection of potential vaccine candidates...

..study was aimed to evaluate VP1-derived chimeric VLPs as tools for hybridoma technology to generate monoclonal antibodies (mAbs) of desired specificity...

..These results are in complete agreement with the results obtained by our recent ICC method using another mAb (ASPM-29) produced against GA-conjugated Spm...

..Therefore, SC142 may become a valuable tool for the immunohistochemical diagnosis and tumor immunoscintigraphy of the gastrointestinal cancer patients...

..CONCLUSIONS: 3G4 localizes specifically to complexes of anionic phospholipids and serum proteins on the surface of vascular endothelial cells in tumors in mice. This results in damage to tumor vasculature and suppression of tumor growth...

..Thus, MAb 806 can be used to target tumor cells containing amplification of the EGFR gene or de2-7 EGFR but does not bind to the wild type EGFR when expressed on the cell surface...

..CONCLUSIONS: Epitope-specific immunization is feasible for active anti-EGFR immunotherapy. The in vitro biologic features of mimotope-induced antibodies are similar to those of the monoclonal antibody cetuximab...

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..METHODS: Monoclonal antibodies directed to purified Cup a 1 were produced...

..These findings demonstrate that IL-21 significantly enhances the NK cell response to Ab-coated targets and suggest that IL-21 would be an effective adjuvant to administer in combination with therapeutic mAbs...

..Consequently, it has been suggested that it may be difficult to prepare monoclonal antibodies (MAbs) that are specific for the individual receptor types...

..From the structural data obtained in this study it is concluded that glycosylation of the antibody may be important in the clinical outcome of targeted anti-cancer immunotherapy...

..Using a conventional immunization protocol, twenty stable murine monoclonal antibodies (MAbs) producing cell lines to DNP were generated...

..IL-8(77) was detected in plasma and cerebrospinal fluid (CSF) from patients with sepsis and meningitis...

..Using Stachybotrys chartarum as a model fungus we sought to demonstrate that monoclonal antibodies (mAbs) can provide species-specific diagnostic reagents and also be used to investigate immunological ..

..In this work, the reactivity of five anti-Streptococcus mutans monoclonal antibodies (MoAb) (24A, 56G, C8, E8 and F6) was tested against oral streptococci, cardiac antigens and skeletal and ..

b>Monoclonal antibodies (mAbs) and polyclonal antibodies (pAbs) against human interferon gamma (IFN-gamma) were produced and used for development of a sensitive enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation ..

This paper describes the production and characterization of RVFV monoclonal antibodies. The characteristics of 32 out of 55 ELISA and/or IFA positive monoclonal antibodies were determined, including the RVFV components against which they ..

Polyclonal and monoclonal antibodies (MAbs) have been raised against B16F10 cells collected from growing tumors in vivo or grown in culture media supplemented with normal mouse serum to avoid xenogeneic reactivity...

..The ability of the corresponding monoclonal antibodies (Mabs) to bind multiple antigens was investigated using two techniques...

..CONCLUSION: These findings are indicative of antigen-dependent network perturbation being a causative factor in polyclonal lymphocyte activation...

..with recombinant TmpA were fused with mouse myeloma cells (P3/X63-Ag8), and five hybridomas secreting monoclonal antibodies were obtained...

..in CIA was demonstrated by arthritis development in BALB/c mice upon the combined transfer of 2 mouse monoclonal antibodies specific for precisely mapped conformational CII epitopes (amino acid residues 359-369 [C1(III)] and 551-..

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PURPOSE: We have demonstrated previously that a mixture of three anti-Her-2 monoclonal antibodies (MAbs) that bind to different epitopes on the extracellular domain of Her-2 expressed on a human breast cancer cell line has more potent ..

..Considering their lack of immunogenicity to humans, these CEA-specific HmAbs may be useful for immunotherapeutic approaches as well as for immunodiagnosis...

..These findings are important for further clarification of GBS immunology and immunoprotection in humans...

INTRODUCTION: Mouse monoclonal antibodies (mAbs) with the ability to inhibit thyrotropin (TSH) binding to the TSH receptor (TSHR) are useful tools to study TSH-TSHR interaction...

..Appl. Environ. Microbiol. 60:3120-3127, 1997). To our best knowledge, this is the first example of an immunological identification system that uses p60-recognizing MAbs...

..Anti-idiotypic antibodies to PAb-421 (sampled as monoclonal antibodies) could also bind DNA...

..Previous studies revealed that several mouse monoclonal antibodies (MAbs) recognized U1A as part of the U1snRNP, while MAb 12E12 was unique in that it recognized an epitope ..

..In the present study, we prepared several monoclonal antibodies (mAbs) specific for ML 14-69 and examined their antigen specificities in detail, to characterize the ..

..The antigen determinant recognized by the antibody has been located within hexapeptide [PGQGQQ] or / and nonapeptide [GYYPTSPQQ] in the central repetitive region of HMW-GS...

..These results support the hypothesis that specific monoclonal antibody bound to a bacterial vaccine can alter the quality of the humoral immune response to that organism...

..The high specificity of serogroup- or species-specific mAbs obtained in ELISA suggests that they may be effective for serotyping of Y. pseudotuberculosis strains or differentiation from other pathogenic yersiniae...

In this study we describe the generation of monoclonal antibodies (mAbs), which recognize different epitopes of the equine IgE constant heavy chain. Equi-murine recombinant IgE (rIgE), composed of the murine V(H)186...

..Likewise, the identified epitope may be useful for the detection of p26 antibodies in HIV-2-infected individuals...

..Here, we report on two functional mouse anti-human CD40L monoclonal antibodies 1B1 and 4F1 characterized by flow cytometry, Western blotting, and competition assay...

..containing the capsid protein of BIV expressed in Escherichia coli was used to immunize mice and produce monoclonal antibodies. Six hybridomas specific for BIV capsid protein were identified, and one antibody, designated 10H1, was ..

Hybridomas secreting immunoglobulin A (IgA) monoclonal antibodies (MAbs) against Salmonella enteritidis lipopolysaccharide (LPS) were generated after mucosal immunization of BALB/c mice with heat killed bacteria...

..In this study, we describe a panel of specific monoclonal antibodies that react with the beta(1C) cytodomain...

This report describes an assay for comparison of epitope specificity in groups of monoclonal antibodies against a given antigen. The only prerequisite is the biotin-labeled antigen...

..Thus, the in vitro actions of IgA described may also play a regulatory role for mouse macrophages in vivo...

b>Monoclonal antibodies (mAbs) recognizing native epitopes are an important tool for functional and structural studies of proteins, yet they have rarely been used with transport proteins...

Four monoclonal antibodies (mAbs) (M1357, M1360, M1823 and M1825) which reacted with Campylobacter fetus lipopolysaccharide (LPS) core region epitopes were produced and characterized. Reactivity of these mAbs with C...

..The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques...

Sixteen monoclonal antibodies (MAbs) specific to 2 urochordate genera (Botryllus schlosseri and Botrylloides) intracellular epitopes were generated in mice immunized with a mixture of fresh and paraformaldehyde-fixed cells obtained from ..

..We have produced isotype-specific mouse monoclonal antibodies (MAbs) in order to investigate the roles of HCAbs in camelid immunity...

OBJECTIVE: To prepare and identify the monoclonal antibodies (mAb) against human zeta globin chain...

..Furthermore, EDG is essential for angiogenesis. We generated two anti-EDG monoclonal antibodies (mAbs), termed SN6a and SN6h, defining different epitopes of EDG and developed a double-antibody sandwich ..

..In contrast, the 2-11G antibody neutralized only human rotaviruses with the G1 serotype. The concentration of antibodies required for 50% neutralization ranged from 0.8 to 20 micro g/ml...

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..5 mug/mL. CONCLUSIONS: Phospho-EGFR/phospho-MAPK could be useful clinical biomarkers to assess EGFR inhibition by cetuximab...

..CONCLUSION: The results reported in this study suggest that a monoclonal antitenascin antibody mixture can improve tumor targeting. This strategy could represent progress for therapeutic approaches such as PAGRIT...

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Two monoclonal antibodies against tumor necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL), designated VI10E and III6F, have been generated...

Five monoclonal antibodies (mAbs), 1G8, 1H9, 2D2, 2D3, and 2F5, against Scophthalmus maximus rhabdovirus (SMRV) were prepared...

..We investigate here the functional effects of three monoclonal antibodies (MoAbs) raised against murine E-selectin (9A9, 10E6, and 10E9...