histamine release


Summary: The secretion of histamine from mast cell and basophil granules by exocytosis. This can be initiated by a number of factors, all of which involve binding of IgE, cross-linked by antigen, to the mast cell or basophil's Fc receptors. Once released, histamine binds to a number of different target cell receptors and exerts a wide variety of effects.

Top Publications

  1. Li Y, Lee S, Le Q, Kim M, Kim S. Anti-allergic effects of phlorotannins on histamine release via binding inhibition between IgE and Fc epsilonRI. J Agric Food Chem. 2008;56:12073-80 pubmed publisher
    ..were assessed on human basophilic leukemia (KU812) and rat basophilic leukemia (RBL-2H3) cell lines using a histamine release assay. Both compounds 1 and 3 exhibited a significant inhibitory activity against histamine release...
  2. Mari A, Ooievaar De Heer P, Scala E, Giani M, Pirrotta L, Zuidmeer L, et al. Evaluation by double-blind placebo-controlled oral challenge of the clinical relevance of IgE antibodies against plant glycans. Allergy. 2008;63:891-6 pubmed publisher
    ..The potency of IgE to induce mediator release was assessed by basophil histamine release and skin prick tests (SPTs)...
  3. Dutta A, Deshpande S. Indian red scorpion venom-induced augmentation of cardio-respiratory reflexes and pulmonary edema involve the release of histamine. Toxicon. 2011;57:193-8 pubmed publisher
    ..9 cells/mm³). These observations indicate that venom-induced pulmonary edema and augmentation of PBG reflexe are mediated through mast cells by involving H? receptors. ..
  4. Galatowicz G, Ajayi Y, Stern M, Calder V. Ocular anti-allergic compounds selectively inhibit human mast cell cytokines in vitro and conjunctival cell infiltration in vivo. Clin Exp Allergy. 2007;37:1648-56 pubmed
    ..cell-derived (CBMC) and conjunctival biopsy-derived MCs were stimulated via FcepsilonRI, degranulation and histamine release were assayed at 1 h and cytokine secretion at 24 h using multiplex arrays...
  5. Duffy S, Cruse G, Cockerill S, Brightling C, Bradding P. Engagement of the EP2 prostanoid receptor closes the K+ channel KCa3.1 in human lung mast cells and attenuates their migration. Eur J Immunol. 2008;38:2548-56 pubmed publisher
    ..1 in HLMC and attenuates both chemokine- and PGE(2)-dependent HLMC migration. EP(2) receptor agonists with K(Ca)3.1 modulating function may be useful for the treatment of mast cell-mediated disease. ..
  6. Kumari D, Arora N, Kasera R, Sridhara S, Kumar R, Singh B. Isolation and characterization of a 28 kDa major allergen from blackgram (Phaseolus mungo). Immunobiology. 2012;217:895-904 pubmed publisher
    ..purified protein was characterized by indirect ELISA, immunoblot, ELISA inhibition, SPTs, stripped basophil histamine release, lymphoproliferation assay and digestibility assay...
  7. Wei J, Wei X, Mo Y, He S. Induction of microvascular leakage and histamine release by promutoxin, an Arg49 phospholipase A2. Toxicon. 2010;55:888-96 pubmed publisher
    ..Moreover, promutoxin was found to induce histamine release from human colon, lung and tonsil mast cells, and both metabolic inhibitors and pertussis toxin were capable ..
  8. Zoccatelli G, Pokoj S, Foetisch K, Bartra J, Valero A, Del Mar San Miguel Moncin M, et al. Identification and characterization of the major allergen of green bean (Phaseolus vulgaris) as a non-specific lipid transfer protein (Pha v 3). Mol Immunol. 2010;47:1561-8 pubmed publisher
    ..Three natural LTPs (nLTPs) were further purified from GB fruit by chromatography. In vitro histamine release test was applied to compare the allergenic potency of rLTPs and nLTPs. ..
  9. Hamilton R, Franklin Adkinson N. In vitro assays for the diagnosis of IgE-mediated disorders. J Allergy Clin Immunol. 2004;114:213-25; quiz 226 pubmed
    ..The current challenge is to translate the quantitative IgE antibody results into a more accurate diagnosis of allergic disease. ..

More Information


  1. Weller K, Foitzik K, Paus R, Syska W, Maurer M. Mast cells are required for normal healing of skin wounds in mice. FASEB J. 2006;20:2366-8 pubmed
    ..Taken together, our findings indicate that MC activation and histamine release are required for normal cutaneous wound healing.
  2. Yang W, Wardlaw A, Bradding P. Attenuation of human lung mast cell degranulation by bronchial epithelium. Allergy. 2006;61:569-75 pubmed
    ..Histamine was measured by radioenzymatic assay. After co-culture for 30 min, IgE-dependent histamine release from HLMC was identical on both BEAS-2B and plastic...
  3. Wei J, Mo Y, Qiao L, Wei X, Chen H, Xie H, et al. Potent histamine-releasing activity of atrahagin, a novel snake venom metalloproteinase. Int J Biochem Cell Biol. 2006;38:510-20 pubmed
    ..activity, atrahagin potently activated human colon, lung and tonsil mast cells with the net histamine release being 25.9+/-4.4, 17.0+/-1.9, 13.2+/-3.6%, respectively...
  4. Liu T, Bai Z, Pang X, Chai Z, Jiang F, Ji Y. Degranulation of mast cells and histamine release involved in rat pain-related behaviors and edema induced by scorpion Buthus martensi Karch venom. Eur J Pharmacol. 2007;575:46-56 pubmed
    In the present study, it was investigated whether the degranulation of mast cells and histamine release were involved in rat pain-related behaviors and edema induced by the venom of scorpion Buthus martensi Karch (BmK) or not...
  5. Asero R, Lorini M, Chong S, Zuberbier T, Tedeschi A. Assessment of histamine-releasing activity of sera from patients with chronic urticaria showing positive autologous skin test on human basophils and mast cells. Clin Exp Allergy. 2004;34:1111-4 pubmed
    ..patients with chronic urticaria (CU) positive on the autologous serum skin test (ASST) are able to induce histamine release in vitro...
  6. Kay L, Yeo W, Peachell P. Prostaglandin E2 activates EP2 receptors to inhibit human lung mast cell degranulation. Br J Pharmacol. 2006;147:707-13 pubmed
    ..PGF(2alpha), the IP agonist iloprost and the TP agonist U-46619 were ineffective inhibitors of IgE-mediated histamine release from mast cells. PGE2 induced a concentration-dependent increase in intracellular cAMP levels in mast cells...
  7. Na H, Moon P, Ko S, Lee H, Jung H, Hong S, et al. Sargassum hemiphyllum inhibits atopic allergic reaction via the regulation of inflammatory mediators. J Pharmacol Sci. 2005;97:219-26 pubmed
    ..Our results suggest that SH has an inhibitory effect on the atopic allergic reaction and thus this may be useful in the treatment of allergic inflammatory diseases, such as atopic dermatitis...
  8. Mittag D, Akkerdaas J, Ballmer Weber B, Vogel L, Wensing M, Becker W, et al. Ara h 8, a Bet v 1-homologous allergen from peanut, is a major allergen in patients with combined birch pollen and peanut allergy. J Allergy Clin Immunol. 2004;114:1410-7 pubmed
    ..We recently described patients with soybean allergy mainly mediated by cross-reactivity to birch pollen allergens. A majority of those patients were reported to have peanut allergy...
  9. Law M, Morales J, Mottram L, Iyer A, Peterson B, August A. Structural requirements for the inhibition of calcium mobilization and mast cell activation by the pyrazole derivative BTP2. Int J Biochem Cell Biol. 2011;43:1228-39 pubmed publisher
    ..In vivo, BTP2 inhibits antigen-induced histamine release. Structure-activity relationship analysis indicates that substitution at the C3 or C5 position of the ..
  10. de Oliveira D, Luchini A, Seito L, Gomes J, Crespo Lopez M, Di Stasi L. Cordia verbenacea and secretion of mast cells in different animal species. J Ethnopharmacol. 2011;135:463-8 pubmed publisher
    ..verbenacea on histamine release (in vitro and in vivo studies) from different types of mast cells induced by chemical agents using several ..
  11. Rama T, Côrte Real I, Gomes P, Escribano L, Fernandes M. Mastocytosis: oral implications of a rare disease. J Oral Pathol Med. 2011;40:441-50 pubmed publisher
    ..This review aims to briefly summarize the potential ways in which mast cell disease may affect the oral cavity and the dental management of mastocytosis affected patients. ..
  12. van der Meide N, Meulenbroeks C, van Altena C, Schurink A, Ducro B, Wagner B, et al. Culicoides obsoletus extract relevant for diagnostics of insect bite hypersensitivity in horses. Vet Immunol Immunopathol. 2012;149:245-54 pubmed publisher
    ..Basophil degranulation was assessed by histamine release test (HRT) after stimulation with these extracts at 5, 0.5 and 0.05 ?g/ml...
  13. Sakuma M, Shirai Y, Yoshino K, Kuramasu M, Nakamura T, Yanagita T, et al. Novel PKC?-mediated phosphorylation site(s) on cofilin and their potential role in terminating histamine release. Mol Biol Cell. 2012;23:3707-21 pubmed
  14. Koketsu R, Suzukawa M, Kawakami A, Komiya A, Ra C, Yamamoto K, et al. Activation of basophils by stem cell factor: comparison with insulin-like growth factor-I. J Investig Allergol Clin Immunol. 2008;18:293-9 pubmed
    ..SCF enhanced human basophil histamine release elicited by some, but not all, secretagogues; degranulation in response to IgE- or FcepsilonRI-mediated ..
  15. de F Carvalho V, Campos L, Farias Filho F, Florim L, Barreto E, Pirmez C, et al. Suppression of allergic inflammatory response in the skin of alloxan-diabetic rats: relationship with reduced local mast cell numbers. Int Arch Allergy Immunol. 2008;147:246-54 pubmed publisher
    ..Also, fragments of the dorsal subcutaneous tissue from diabetic sensitized rats showed a reduction in histamine release after stimulation with antigen in vitrowhen compared with fragments obtained from nondiabetic sensitized ..
  16. Smith C, Bradding P, Neill D, Baxendale H, Felici F, Andrew P. Novel immunogenic peptides elicit systemic anaphylaxis in mice: implications for peptide vaccines. J Immunol. 2011;187:1201-6 pubmed publisher
    ..These results have important implications for both the study of allergens and the development of future peptide-based therapies. ..
  17. Wirotesangthong M, Inagaki N, Tanaka H, Thanakijcharoenpath W, Nagai H. Inhibitory effects of Piper betle on production of allergic mediators by bone marrow-derived mast cells and lung epithelial cells. Int Immunopharmacol. 2008;8:453-7 pubmed publisher
    ..The results suggest that P. betle may offer a new therapeutic approach for the control of allergic diseases through inhibition of production of allergic mediators...
  18. Hu J, He G, Li T, Meng J, Ma X, Jia M, et al. Exocytosis and mobility of histamine vesicles exhibit no difference to that of histamine-negative vesicles in guinea pig superior cervical ganglion neurons. Neuroreport. 2011;22:274-81 pubmed publisher
    ..In conclusion, these findings provide further characters of histamine as a sympathetic neurotransmitter. ..
  19. Matsui K, Wirotesangthong M, Thanakijcharoenpath W, Mungmee C, Nishikawa A. Inhibitory effects of Schefflera leucantha extract on production of allergic mediators by Langerhans cells and mast cells. J Investig Allergol Clin Immunol. 2010;20:463-8 pubmed
    ..by epidermal Langerhans cells (LCs) stimulated with peptidoglycan (PEG) from Staphylococcus aureus and histamine release from mast cells...
  20. Ozseker F, Buyukozturk S, Gelincik A, Depboylu B, Genc S, Giris M, et al. Neurotrophins: are they meaningful in chronic spontaneous urticaria?. Asian Pac J Allergy Immunol. 2008;26:83-8 pubmed
    ..Our study suggests that chronic spontaneous urticaria is linked with changes serum nerve growth factor levels, and that the deregulation of neurotrophins may contribute to urticaria pathophysiology. ..
  21. Hochwallner H, Schulmeister U, Swoboda I, Focke Tejkl M, Civaj V, Balic N, et al. Visualization of clustered IgE epitopes on alpha-lactalbumin. J Allergy Clin Immunol. 2010;125:1279-1285.e9 pubmed publisher
    ..dichroism, and its allergenic activity was studied by using microarray technology, as well as in a basophil histamine release assay. IgE epitope mapping was performed with synthetic peptides...
  22. Bitziou E, O Hare D, Patel B. Spatial changes in acid secretion from isolated stomach tissue using a pH-histamine sensing microarray. Analyst. 2010;135:482-7 pubmed publisher
    ..These results identify great possibilities in applying these multi-sensing devices as a long-term stable personalised diagnostic tool for pharmacological screening and disease status. ..
  23. Venkatesh P, Mukherjee P, Kumar S, Nema N, Bandyopadhyay A, Fukui H, et al. Mast cell stabilization and antihistaminic potentials of Curculigo orchioides rhizomes. J Ethnopharmacol. 2009;126:434-6 pubmed publisher
    ..Fam. Amaryllidaceae) has been reported to possess immunostimulant, and anti-inflammatory potentials. In Indian traditional system of medicine it is also used as anti-asthmatic and anti-inflammatory...
  24. Coulson J, Thompson J. Paracetamol (acetaminophen) attenuates in vitro mast cell and peripheral blood mononucleocyte cell histamine release induced by N-acetylcysteine. Clin Toxicol (Phila). 2010;48:111-4 pubmed publisher
    ..Paracetamol (acetaminophen) attenuates in vitro mast cell and PBMC cell histamine release induced by NAC.
  25. Macglashan D, Ishmael S, MacDonald S, Langdon J, Arm J, Sloane D. Induced loss of Syk in human basophils by non-IgE-dependent stimuli. J Immunol. 2008;180:4208-17 pubmed
    ..of Syk and c-Cbl, was inhibited by a third generation Syk inhibitor with an expected IC(50), and induced histamine release in strict proportion to release induced by anti-IgE Ab...
  26. Konstantinou G, Asero R, Ferrer M, Knol E, Maurer M, Raap U, et al. EAACI taskforce position paper: evidence for autoimmune urticaria and proposal for defining diagnostic criteria. Allergy. 2013;68:27-36 pubmed publisher
    ..chronic urticaria (CU) patient sera have been demonstrated against IgE and Fc?RI? by basophil and mast cell histamine release assays and by basophil activation assays...
  27. Raithel M, Hagel A, Zopf Y, Bijlsma P, de Rossi T, Gabriel S, et al. Analysis of immediate ex vivo release of nitric oxide from human colonic mucosa in gastrointestinally mediated allergy, inflammatory bowel disease and controls. J Physiol Pharmacol. 2012;63:317-25 pubmed
    ..Incubation with anti-human IgE antibodies or allergens produced a ninefold increase in histamine release in gastrointestinally mediated allergy (p<0...
  28. Kaszaki J, Czobel M, Szalay L, Nagy S, Boros M. Endothelin-1 induces organ-specific histamine liberation and neutrophil granulocyte accumulation in the rat. Inflamm Res. 2008;57:396-402 pubmed publisher
    ..ET-1-induced direct effects are predominating in pulmonary neutrophil activation, while MC-associated secondary changes play important roles in intestinal granulocyte recruitment. ..
  29. Sun J, Wu Y, Wang J, Ma F, Liu X, Li Q. Novel translationally controlled tumor protein homologue in the buccal gland secretion of Lampetra japonica. Biochimie. 2008;90:1760-8 pubmed publisher
    ..A characterization study shows that recombinant TCTP has histamine-releasing function in vitro. It mediated histamine release from rat basophilic leukemia (RBL-2H3) cells...
  30. Wierzbicki M, Brzezińska Błaszczyk E. Diverse effects of bacterial cell wall components on mast cell degranulation, cysteinyl leukotriene generation and migration. Microbiol Immunol. 2009;53:694-703 pubmed publisher
    ..We found that PGN, LPS and LAM all failed to induce mast cell degranulation and histamine release. At the same time, activation of mast cells with these bacterial antigens resulted in generation and release ..
  31. Chen E, Garnica M, Wang Y, Mintz A, Chen C, Chin W. A mixture of anatase and rutile TiO? nanoparticles induces histamine secretion in mast cells. Part Fibre Toxicol. 2012;9:2 pubmed publisher
    ..Our data suggests that systemic circulation of NPs may prompt histamine release at different locales causing abnormal inflammatory diseases...
  32. Lee J, Lim K. Inhibitory effect of phytoglycoprotein (24kDa) on allergy-related factors in compound 48/80-induced mast cells in vivo and in vitro. Int Immunopharmacol. 2010;10:591-9 pubmed publisher
    ..Taken together, we speculate that the ZPDC glycoprotein might be one component found in natural products that has the ability to prevent dysfunction in the immune system caused by several different allergens...
  33. Ohno S, Yakabi K, Ro S, Ochiai M, Onouchi T, Sakurada T, et al. Apelin-12 stimulates acid secretion through an increase of histamine release in rat stomachs. Regul Pept. 2012;174:71-8 pubmed publisher
    ..Furthermore, isolated vascularly perfused rat stomachs were prepared to examine the effect of apelin on histamine release, which was assayed in the effluent by radioimmunoassay...
  34. Nugroho A, Ikawati Z, Sardjiman -, Maeyama K. Effects of benzylidenecyclopentanone analogues of curcumin on histamine release from mast cells. Biol Pharm Bull. 2009;32:842-9 pubmed
    ..present study, fourteen benzylidenecyclopentanone analogues of curcumin were studied for their effects on histamine release from rat basophilic leukemia (RBL-2H3) cells...
  35. Bossi F, Frossi B, Radillo O, Cugno M, Tedeschi A, Riboldi P, et al. Mast cells are critically involved in serum-mediated vascular leakage in chronic urticaria beyond high-affinity IgE receptor stimulation. Allergy. 2011;66:1538-45 pubmed publisher
    ..Autoimmune aetiology can be ascribed to 45% of patients with CU, and basophil histamine release is positive in 40% of cases...
  36. Lee J, Kim C. Arctigenin, a phenylpropanoid dibenzylbutyrolactone lignan, inhibits type I-IV allergic inflammation and pro-inflammatory enzymes. Arch Pharm Res. 2010;33:947-57 pubmed publisher
    ..o., and compound 48/80-induced histamine release from rat peritoneal mast cells at 10 microM. Arctigenin (15 mg/kg, p.o...
  37. Egido V, Fuentes A, Buisan L. [Systemic mastocytosis and anesthesia: a case report]. Rev Esp Anestesiol Reanim. 2008;55:61-2 pubmed
  38. Bartley K, Nisbet A, Offer J, Sparks N, Wright H, Huntley J. Histamine release factor from Dermanyssus gallinae (De Geer): characterization and in vitro assessment as a protective antigen. Int J Parasitol. 2009;39:447-56 pubmed publisher
    A cDNA encoding a 174-amino-acid orthologue of a tick histamine release factor (HRF) was identified from the haematophagous poultry red mite Dermanyssus gallinae. The predicted D...
  39. Rosenthal A, Blønd M. [The enzyme papain in industry and food causes allergic sensitization]. Ugeskr Laeger. 2008;170:3127-9 pubmed
    ..10 out of 22 employees reported work-related ocular itching and symptoms of rhinitis. A Histamine Release Test showed that three employees were sensitized to papain...
  40. Haruki Y, Takatori S, Hattori S, Zamami Y, Koyama T, Tangsucharit P, et al. Adrenergic stimulation-released histamine taken-up in adrenergic nerves induces endothelium-dependent vasodilation in rat mesenteric resistance arteries. J Pharmacol Sci. 2012;118:537-42 pubmed
    ..These results suggest that histamine taken up by and released from adrenergic nerves by PNS causes endothelium-dependent vasodilation in rat mesenteric arteries. ..
  41. Inami Y, Andoh T, Sasaki A, Kuraishi Y. Topical surfactant-induced pruritus: involvement of histamine released from epidermal keratinocytes. J Pharmacol Exp Ther. 2013;344:459-66 pubmed publisher
    ..The present results suggest that sodium laurate induced delayed scratching at an alkaline pH through the increased production of histamine in keratinocytes, which may be due to enhanced processing of 74-kDa to 53-kDa HDC. ..
  42. Sato S, Tachimoto H, Shukuya A, Ogata M, Komata T, Imai T, et al. Utility of the peripheral blood basophil histamine release test in the diagnosis of hen's egg, cow's milk, and wheat allergy in children. Int Arch Allergy Immunol. 2011;155 Suppl 1:96-103 pubmed publisher
    ..The utility of the histamine release test (HRT) in the diagnosis of childhood FA has not been fully examined.
  43. Kida T, Fujii A, Sakai O, Iemura M, Atsumi I, Wada T, et al. Bepotastine besilate, a highly selective histamine H(1) receptor antagonist, suppresses vascular hyperpermeability and eosinophil recruitment in in vitro and in vivo experimental allergic conjunctivitis models. Exp Eye Res. 2010;91:85-91 pubmed publisher
    ..05%. Chemotaxis of guinea pig peritoneal eosinophils and histamine release from rat peritoneal mast cells in vitro were also inhibited by addition of bepotastine...
  44. Weller K, Maurer M. Desloratadine inhibits human skin mast cell activation and histamine release. J Invest Dermatol. 2009;129:2723-6 pubmed publisher
  45. Konrad F, Schroeder T. Anaesthesia in patients with mastocytosis. Acta Anaesthesiol Scand. 2009;53:270-1 pubmed publisher
  46. Altrich M, Halsey J, Altman L. Comparison of the in vivo autologous skin test with in vitro diagnostic tests for diagnosis of chronic autoimmune urticaria. Allergy Asthma Proc. 2009;30:28-34 pubmed publisher
    ..We performed skin tests and two functional in vitro tests, basophil histamine release, and CD63 up-regulation to detect autoantibodies relevant to autoimmune urticaria...
  47. Wimazal F, Baumgartner C, Sonneck K, Zauner C, Geissler P, Schur S, et al. Mixed-lineage eosinophil/basophil crisis in MDS: a rare form of progression. Eur J Clin Invest. 2008;38:447-55 pubmed publisher
    ..1%) were found to have massive eosinophilia ( >or= 20%), whereas mixed-lineage crisis was detected in none of them. Mixed basophil/eosinophil crisis may develop in patients with MDS but is an extremely rare event. ..
  48. Baker R, Vasagar K, Ohameje N, Gober L, Chen S, Sterba P, et al. Basophil histamine release activity and disease severity in chronic idiopathic urticaria. Ann Allergy Asthma Immunol. 2008;100:244-9 pubmed publisher
    ..To evaluate CIU disease severity in relation to basophil histamine release (HR) characteristics. Patients with CIU were recruited from allergy and dermatology clinics...
  49. Baroody F, Foster K, Markaryan A, deTineo M, Naclerio R. Nasal ocular reflexes and eye symptoms in patients with allergic rhinitis. Ann Allergy Asthma Immunol. 2008;100:194-9 pubmed publisher
    ..This reflex is reduced by an H1-receptor antagonist applied at the site of the challenge. The eye symptoms associated with allergic rhinitis probably arise, in part, from a naso-ocular reflex. ..
  50. Iwata N, Kitanaka S. Tetracyclic chromane derivatives from Rhododendron anthopogonoides. J Nat Prod. 2010;73:1203-6 pubmed publisher
    ..Compounds 1 and 2 inhibited compound 48/80-induced histamine release from rat peritoneal mast cells.
  51. Dev S, Mizuguchi H, Das A, Maeyama K, Horinaga S, Kato S, et al. Kujin suppresses histamine signaling at the transcriptional level in toluene 2,4-diisocyanate-sensitized rats. J Pharmacol Sci. 2009;109:606-17 pubmed
    ..Suppression of Th2-cytokine signaling by Kujin also suggests that it could affect the histamine-cytokine network. ..
  52. Hadzijusufovic E, Peter B, Herrmann H, Rülicke T, Cerny Reiterer S, Schuch K, et al. NI-1: a novel canine mastocytoma model for studying drug resistance and IgER-dependent mast cell activation. Allergy. 2012;67:858-68 pubmed publisher
    ..Kit mutations supposedly contribute to abnormal growth and drug resistance in these patients...
  53. Pakravan N, Waring W, Sharma S, Ludlam C, Megson I, Bateman D. Risk factors and mechanisms of anaphylactoid reactions to acetylcysteine in acetaminophen overdose. Clin Toxicol (Phila). 2008;46:697-702 pubmed publisher
    ..Severity of adverse effects was associated with histamine release: AUC for change from baseline histamine was -6 ng/mL min (-60 to 11 ng/mL min) in the minimal group, 26 ng/..