acridine orange

Summary

Summary: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.

Top Publications

  1. Bhakdi S, Sratongno P, Chimma P, Rungruang T, Chuncharunee A, Neumann H, et al. Re-evaluating acridine orange for rapid flow cytometric enumeration of parasitemia in malaria-infected rodents. Cytometry A. 2007;71:662-7 pubmed
    ..We re-evaluated the use of the DNA/RNA discriminating vital fluorochrome acridine orange (AO) for rapid flow cytometric enumeration of parasitemia in rodents...
  2. Krolenko S, Adamyan S, Belyaeva T, Mozhenok T. Acridine orange accumulation in acid organelles of normal and vacuolated frog skeletal muscle fibres. Cell Biol Int. 2006;30:933-9 pubmed
    ..b>Acridine orange (AO) was used to evaluate acid compartments, while a lipophilic styryl dye, RH 414, was employed to stain the ..
  3. Matsubara T, Kusuzaki K, Matsumine A, Murata H, Satonaka H, Shintani K, et al. A new therapeutic modality involving acridine orange excitation by photon energy used during reduction surgery for rhabdomyosarcomas. Oncol Rep. 2009;21:89-94 pubmed
    ..involving photodynamic surgery (PDS), photodynamic therapy (PDT) and radiodynamic therapy (RDT) using acridine orange (AO)...
  4. Lobasso S, Saponetti M, Polidoro F, Lopalco P, Urbanija J, Kralj Iglic V, et al. Archaebacterial lipid membranes as models to study the interaction of 10-N-nonyl acridine orange with phospholipids. Chem Phys Lipids. 2009;157:12-20 pubmed publisher
    The dye 10-N-nonyl acridine orange (NAO) is used to label cardiolipin domains in mitochondria and bacteria. The present work represents the first study on the binding of NAO with archaebacterial lipid membranes...
  5. Rodriguez M, Azizuddin K, Zhang P, Chiu S, Lam M, Kenney M, et al. Targeting of mitochondria by 10-N-alkyl acridine orange analogues: role of alkyl chain length in determining cellular uptake and localization. Mitochondrion. 2008;8:237-46 pubmed publisher
    10-N-Nonyl acridine orange (NAO) is used as a mitochondrial probe because of its high affinity for cardiolipin (CL). Targeting of NAO may also depend on mitochondrial membrane potential...
  6. Chávez E, Zazueta C, García N, Martínez Abundis E, Pavón N, Hernández Esquivel L. Titration of cardiolipin by either 10-N-nonyl acridine orange or acridine orange sensitizes the adenine nucleotide carrier to permeability transition. J Bioenerg Biomembr. 2008;40:77-84 pubmed publisher
    ..This work shows that 10-N-nonyl acridine orange and acridine orange, probes for this phospholipid, modify the sensitivity of the translocase to ..
  7. Nakamura T, Kusuzaki K, Matsubara T, Matsumine A, Murata H, Uchida A. A new limb salvage surgery in cases of high-grade soft tissue sarcoma using photodynamic surgery, followed by photo- and radiodynamic therapy with acridine orange. J Surg Oncol. 2008;97:523-8 pubmed publisher
    ..with high-grade malignant sarcomas, we developed and established a new surgical adjuvant therapy using acridine orange (AO) after intra-lesional or marginal resection while sparing normal tissues of major nerves, vessels or ..
  8. Suzuki N, Okada K, Chida S, Komori C, Shimada Y, Suzuki T. Antitumor effect of acridine orange under ultrasonic irradiation in vitro. Anticancer Res. 2007;27:4179-84 pubmed
    ..Sonodynamic synergistic antitumor therapy is a promising new methodology for cancer treatment...
  9. Cavas T. In vivo genotoxicity of mercury chloride and lead acetate: Micronucleus test on acridine orange stained fish cells. Food Chem Toxicol. 2008;46:352-8 pubmed
    ..The results of this study indicate that LA and MC have genotoxic and cytotoxic damage in fish and confirmed that AO staining is a suitable technique for in vivo MN test in fish. ..

More Information

Publications32

  1. Liu D, Baker H. Human sperm bound to the zona pellucida have normal nuclear chromatin as assessed by acridine orange fluorescence. Hum Reprod. 2007;22:1597-602 pubmed
    ..red fluorescence) DNA of sperm in semen, motile sperm selected by CSGC and ZP-bound sperm, was assessed by acridine orange (AO) fluorescence...
  2. Baskic D, Popovic S, Ristic P, Arsenijevic N. Analysis of cycloheximide-induced apoptosis in human leukocytes: fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide. Cell Biol Int. 2006;30:924-32 pubmed
    ..Generally, microscopic examination of acridin orange/ethidium bromide stained cells can be recommended as the most reliable of the methods tested. ..
  3. Ribble D, Goldstein N, Norris D, Shellman Y. A simple technique for quantifying apoptosis in 96-well plates. BMC Biotechnol. 2005;5:12 pubmed
    ..in vitro and to take advantage of the 96-well plate format, we present here a modified ethidium bromide and acridine orange (EB/AO) staining assay, which may be performed entirely in a 96-well plate...
  4. Xu L, Chaudhuri A. Plasmodium yoelii: a differential fluorescent technique using Acridine Orange to identify infected erythrocytes and reticulocytes in Duffy knockout mouse. Exp Parasitol. 2005;110:80-7 pubmed
    ..Fluorochromatic dye like Acridine Orange has been used for instantaneous detection of parasites in RBCs...
  5. Ling E, Shirai K, Kanekatsu R, Kiguchi K. Classification of larval circulating hemocytes of the silkworm, Bombyx mori, by acridine orange and propidium iodide staining. Histochem Cell Biol. 2003;120:505-11 pubmed
    Circulating hemocytes of the silkworm can be classified by fluorescence microscopy following staining with acridine orange and propidium iodide...
  6. Udovich J, Besselsen D, Gmitro A. Assessment of acridine orange and SYTO 16 for in vivo imaging of the peritoneal tissues in mice. J Microsc. 2009;234:124-9 pubmed publisher
    The effect of peritoneal injection of acridine orange and SYTO 16 in mice was investigated. Images of peritoneal tissues stained with these dyes and obtained through a confocal micro-endoscope are presented...
  7. Hein Kristensen L, Wiese L, Kurtzhals J, Staalsoe T. In-depth validation of acridine orange staining for flow cytometric parasite and reticulocyte enumeration in an experimental model using Plasmodium berghei. Exp Parasitol. 2009;123:152-7 pubmed publisher
    ..A published two-channel method using acridine orange offers clear discrimination between the infected and uninfected erythrocytes...
  8. Matsubara T, Kusuzaki K, Matsumine A, Murata H, Nakamura T, Uchida A, et al. Clinical outcomes of minimally invasive surgery using acridine orange for musculoskeletal sarcomas around the forearm, compared with conventional limb salvage surgery after wide resection. J Surg Oncol. 2010;102:271-5 pubmed publisher
    ..and established a new surgical therapy combining photodynamic surgery and radiodynamic therapy using acridine orange (AO) therapy after marginal or intralesional tumor resection, providing excellent limb function to sarcoma ..
  9. Byczkowska A, Kunikowska A, Kazmierczak A. Determination of ACC-induced cell-programmed death in roots of Vicia faba ssp. minor seedlings by acridine orange and ethidium bromide staining. Protoplasma. 2013;250:121-8 pubmed publisher
    Fluorescence staining with acridine orange (AO) and ethidium bromide (EB) showed that nuclei of cortex root cells of 1-aminocyclopropane-1-carboxylic acid (ACC)-treated Vicia faba ssp. minor seedlings differed in color...
  10. Kusuzaki K, Hosogi S, Ashihara E, Matsubara T, Satonaka H, Nakamura T, et al. Translational research of photodynamic therapy with acridine orange which targets cancer acidity. Curr Pharm Des. 2012;18:1414-20 pubmed
    During the past 20 years, we have found that acridine orange (AO) selectively accumulates in musculoskeletal sarcomas in vivo or exerts selective cytocidal effects against sarcoma cells in vitro after illumination of the tumor cells with ..
  11. Keij J, BELL PRINCE C, Steinkamp J. Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green, and MitoTracker Green is affected by mitochondrial membrane potential altering drugs. Cytometry. 2000;39:203-10 pubmed
    ..an assay for the simultaneous analysis of mitochondrial membrane potential and mass using the probes 10-nonyl acridine orange (NAO), MitoFluor Green (MFG), and MitoTracker Green (MTG) in HL60 cells...
  12. Katayose H, Yanagida K, Hashimoto S, Yamada H, Sato A. Use of diamide-acridine orange fluorescence staining to detect aberrant protamination of human-ejaculated sperm nuclei. Fertil Steril. 2003;79 Suppl 1:670-6 pubmed
    ..To investigate the influence of human sperm nuclear chromatin on fertilization...
  13. Jacobson J, Duchen M, Heales S. Intracellular distribution of the fluorescent dye nonyl acridine orange responds to the mitochondrial membrane potential: implications for assays of cardiolipin and mitochondrial mass. J Neurochem. 2002;82:224-33 pubmed
    ..The fluorescent dye, 10 nonyl acridine orange (NAO), has been shown to bind cardiolipin in vitro and is frequently used as a stain in living cells to assay ..
  14. Belicová A, Krizkova L, Nagy M, Krajcovic J, Ebringer L. Phenolic acids reduce the genotoxicity of acridine orange and ofloxacin in Salmonella typhimurium. Folia Microbiol (Praha). 2001;46:511-4 pubmed
    ..PA), caffeic acid (CA), ferulic acid (FA) and gentisic acid (GA) (25-100 nmol/L) had protective effects on acridine orange (AO; 216 mumol/L)- and ofloxacin (3 mumol/L)-induced genotoxicity in Salmonella typhimurium...
  15. Darzynkiewicz Z. Differential staining of DNA and RNA in intact cells and isolated cell nuclei with acridine orange. Methods Cell Biol. 1990;33:285-98 pubmed
  16. Muthulakshmi S, Maharajan K, Habibi H, Kadirvelu K, Venkataramana M. Zearalenone induced embryo and neurotoxicity in zebrafish model (Danio rerio): Role of oxidative stress revealed by a multi biomarker study. Chemosphere. 2018;198:111-121 pubmed publisher
    ..LDH) and Nitric oxide (NO)), neurotoxicity (acetylcholinesterase (AChE)), genotoxicity (comet assay and acridine orange staining (AO)) and histological analysis...
  17. Qian Y, Han Q, Wang L, Guo Q, Wang X, Tu P, et al. Total saponins of Albiziae Cortex show anti-hepatoma carcinoma effects by inducing S phase arrest and mitochondrial apoptosis pathway activation. J Ethnopharmacol. 2018;221:20-29 pubmed publisher
    ..The apoptotic cells were observed by Hoechst 33258 staining and acridine orange (AO)/ethidium bromide (EB) double staining...
  18. Matsumoto H, Fujiwara S, Miyagi H, Nakamura N, Shiga Y, Ohta T, et al. Carbonic Anhydrase Inhibitors Induce Developmental Toxicity During Zebrafish Embryogenesis, Especially in the Inner Ear. Mar Biotechnol (NY). 2017;19:430-440 pubmed publisher
    ..b>Acridine orange/ethidium bromide staining indicated that the hair cell damage in the inner ear and pectral fin is due to ..
  19. Elkady A, Hussein R, El Assouli S. Mechanism of Action of Nigella sativa on Human Colon Cancer Cells: the Suppression of AP-1 and NF-κB Transcription Factors and the Induction of Cytoprotective Genes. Asian Pac J Cancer Prev. 2015;16:7943-57 pubmed
    ..Flow cytometry analyses, cell death detection ELISA, fluorescent stains (Hoechst 33342 and acridine orange/ethidium bromide), DNA laddering and comet assays were carried out to confirm the apoptogenic effects of ..
  20. Cumaoglu A, Ari N, Kartal M, Karasu C. Polyphenolic extracts from Olea europea L. protect against cytokine-induced β-cell damage through maintenance of redox homeostasis. Rejuvenation Res. 2011;14:325-34 pubmed publisher
    ..Apoptosis was quantified by detecting acridine orange/ethidium bromide-stained condensed nuclei under a fluorescent microscope...
  21. Liu C, Lee W, Huang B, Chia Y, Chen Y, Chen Y. 16-Hydroxycleroda-3, 13-dien-15, 16-olide inhibits the proliferation and induces mitochondrial-dependent apoptosis through Akt, mTOR, and MEK-ERK pathways in human renal carcinoma cells. Phytomedicine. 2017;36:95-107 pubmed publisher
    ..The apoptotic features were confirmed by acridine orange and ethidium bromide staining...