Summary: A benzimidazole antifilarial agent; it is fluorescent when it binds to certain nucleotides in DNA, thus providing a tool for the study of DNA replication; it also interferes with mitosis.

Top Publications

  1. Cesarone C, Bolognesi C, Santi L. Improved microfluorometric DNA determination in biological material using 33258 Hoechst. Anal Biochem. 1979;100:188-97 pubmed
  2. Ranjan N, Story S, Fulcrand G, Leng F, Ahmad M, King A, et al. Selective Inhibition of Escherichia coli RNA and DNA Topoisomerase I by Hoechst 33258 Derived Mono- and Bisbenzimidazoles. J Med Chem. 2017;60:4904-4922 pubmed publisher
    ..2-23.4 °C), and cytotoxicity studies show similar variation dependent upon the side chain length. Modeling studies suggest critical interactions between the inhibitor side chain and amino acids of the active site of DNA topoisomerase I. ..
  3. Yakimovich A, Huttunen M, Zehnder B, Coulter L, Gould V, Schneider C, et al. Inhibition of Poxvirus Gene Expression and Genome Replication by Bisbenzimide Derivatives. J Virol. 2017;91: pubmed publisher
    ..Here we identified a small molecule, bisbenzimide (bisbenzimidazole), and its derivatives as potent agents against prototypic poxvirus infection in cell culture...
  4. Chen T. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp Cell Res. 1977;104:255-62 pubmed
  5. Labarca C, Paigen K. A simple, rapid, and sensitive DNA assay procedure. Anal Biochem. 1980;102:344-52 pubmed
  6. Usman A, Ahmad M. Binding of Bisphenol-F, a bisphenol analogue, to calf thymus DNA by multi-spectroscopic and molecular docking studies. Chemosphere. 2017;181:536-543 pubmed publisher
    ..BPF induced DNA cleavage under in vitro conditions by plasmid nicking assay suggested it to be genotoxic. ..
  7. Brama P, TeKoppele J, Bank R, Barneveld A, van Weeren P. Functional adaptation of equine articular cartilage: the formation of regional biochemical characteristics up to age one year. Equine Vet J. 2000;32:217-21 pubmed
    ..As collagen turnover is extremely low at mature age, an undisturbed functional adaptation of the collagen network of articular cartilage at a young age may be of significant importance for future strength and resistance to injury. ..
  8. Gholivand M, Peyman H, Gholivand K, Roshanfekr H, Taherpour A, Yaghoubi R. Experimental and Computational Evidence on the Interaction of Cycloalkyl ?-Aminobisphosphonates with Calf Thymus DNA. DNA Cell Biol. 2017;36:541-551 pubmed publisher