nigericin

Summary

Summary: A polyether antibiotic which affects ion transport and ATPase activity in mitochondria. It is produced by Streptomyces hygroscopicus. (From Merck Index, 11th ed)

Top Publications

  1. Kucejova B, Kucej M, Petrezselyova S, Abelovska L, Tomaska L. A screen for nigericin-resistant yeast mutants revealed genes controlling mitochondrial volume and mitochondrial cation homeostasis. Genetics. 2005;171:517-26 pubmed
    ..Taking advantage of the growth inhibition of yeast cells by electroneutral K(+)/H(+) ionophore nigericin, we screened for genetic mutations that would render cells tolerant to this drug when grown on a nonfermentable ..
  2. Sturz G, Phan T, Mummalaneni S, Ren Z, DeSimone J, Lyall V. The K+-H+ exchanger, nigericin, modulates taste cell pH and chorda tympani taste nerve responses to acidic stimuli. Chem Senses. 2011;36:375-88 pubmed publisher
    ..and chorda tympani (CT) nerve responses was investigated before and after incorporating the K(+)-H(+) exchanger, nigericin, in the apical membrane of taste cells...
  3. Dyve Lingelem A, Bergan J, Sandvig K. Inhibitors of intravesicular acidification protect against Shiga toxin in a pH-independent manner. Traffic. 2012;13:443-54 pubmed publisher
    ..Importantly, our data indicate that proton pump activity is required for efficient endosome-to-Golgi transport of Shiga toxin, although acidification as such does not seem to be required. ..
  4. Margolis L, Novikova I Y, Rozovskaya I, Skulachev V. K+/H+-antiporter nigericin arrests DNA synthesis in Ehrlich ascites carcinoma cells. Proc Natl Acad Sci U S A. 1989;86:6626-9 pubmed
    ..2 or by adding low concentrations of the K+/H+ antiporter, the antibiotic nigericin, at neutral pH. Glucose and anaerobiosis potentiate the nigericin effect...
  5. Lim J, Lee Y, Kim H, Rhyu I, Oh M, Youdim M, et al. Nigericin-induced impairment of autophagic flux in neuronal cells is inhibited by overexpression of Bak. J Biol Chem. 2012;287:23271-82 pubmed publisher
    ..role on autophagy-related events, we treated independent cell lines, including MN9D neuronal cells, with nigericin, a K(+)/H(+) ionophore...
  6. Deng C, Liang Y, Wu M, Feng F, Hu W, Chen L, et al. Nigericin selectively targets cancer stem cells in nasopharyngeal carcinoma. Int J Biochem Cell Biol. 2013;45:1997-2006 pubmed publisher
    ..Here, we used NPC cell lines with high and low proportion of CSCs as models to explore the effect of nigericin, an antibiotic, on CSCs...
  7. Moskvin O, Ivanov B, Ignatova L, Kollmeier M. Light-induced stimulation of carbonic anhydrase activity in pea thylakoids. FEBS Lett. 2000;470:375-7 pubmed
    ..6-8.0 was discovered. This effect was inhibited by nigericin or the lipophilic carbonic anhydrase (CA) inhibitor ethoxyzolamide (EZ), but not by the hydrophilic CA inhibitor,..
  8. Antonopoulos C, Russo H, El Sanadi C, Martin B, Li X, Kaiser W, et al. Caspase-8 as an Effector and Regulator of NLRP3 Inflammasome Signaling. J Biol Chem. 2015;290:20167-84 pubmed publisher
    ..gt;2 h) of LPS-primed caspase-1-deficient (Casp1/11(-/-)) BMDC with the canonical NLRP3 inflammasome agonist nigericin results in release of bioactive IL-1β in conjunction with robust caspase-8 activation...
  9. Bala S, Kombrabail M, Prabhananda B. Effect of phloretin on ionophore mediated electroneutral transmembrane translocations of H(+), K(+) and Na(+) in phospholipid vesicles. Biochim Biophys Acta. 2001;1510:258-69 pubmed
    ..The transporters used in this study are (a) the exchange ionophores: nigericin, monensin; (b) combinations of alkali metal ion carriers, valinomycin or nonactin with weak acids carbonyl ..

More Information

Publications62

  1. Lee S, Zhang Q, Karabucak B, Le A. DPSCs from Inflamed Pulp Modulate Macrophage Function via the TNF-?/IDO Axis. J Dent Res. 2016;95:1274-81 pubmed publisher
    ..tumor necrosis factor ? (TNF-?) secretion in response to stimulation with lipopolysaccharides (LPS) and/or nigericin. However, unlike TNF-?, the secreted level of interleukin 1? was not affected by coculture with DPSCs or I-DPSCs...
  2. Watanabe H, Satake A, Kido Y, Tsuji A. [Development of monoclonal-based enzyme-linked immunosorbent assay and immunochromatographic assay for lasalocid and semduramicin]. Shokuhin Eiseigaku Zasshi. 2004;45:107-12 pubmed
    ..With these kits, the detection limits of lasalocid were 50 ng/mL for standard solution and 125 ng/g for chicken muscle, and those of semduramicin were 10 ng/mL for standard solution and 100 ng/g for chicken muscle. ..
  3. Dalmonte M, Forte E, Genova M, Giuffrè A, Sarti P, Lenaz G. Control of respiration by cytochrome c oxidase in intact cells: role of the membrane potential. J Biol Chem. 2009;284:32331-5 pubmed publisher
    ..A high control coefficient value (C(IV) = 0.85) was also measured in the presence of nigericin, i.e. when Delta psi is maximal, and in the presence of nigericin and valinomycin (C(IV) = 0...
  4. Mariathasan S, Weiss D, Newton K, McBride J, O Rourke K, Roose Girma M, et al. Cryopyrin activates the inflammasome in response to toxins and ATP. Nature. 2006;440:228-32 pubmed
    ..The release of IL-1beta in response to nigericin, a potassium ionophore, and maitotoxin, a potent marine toxin, was also found to be dependent on cryopyrin...
  5. Suffrian K, Schulz K, Gutowska M, Riebesell U, Bleich M. Cellular pH measurements in Emiliania huxleyi reveal pronounced membrane proton permeability. New Phytol. 2011;190:595-608 pubmed publisher
    ..DIDS slightly reduced the effect of pH(e) on pH(i). • The data for the first time show the occurrence of a proton permeation pathway in E. huxleyi plasma membrane. pH(i) homeostasis involves a DIDS-sensitive mechanism. ..
  6. Trubitsin B, Mamedov M, Vitukhnovskaya L, Semenov A, Tikhonov A. EPR study of light-induced regulation of photosynthetic electron transport in Synechocystis sp. strain PCC 6803. FEBS Lett. 2003;544:15-20 pubmed
    ..In addition to the linear photosynthetic electron transport, cyanobacteria were capable of maintaining alternative pathways involving cyclic electron transfer around PS 1 and respiratory chains. ..
  7. Janda T, Szalai G, Papp N, Pál M, Páldi E. Effects of freezing on thermoluminescence in various plant species. Photochem Photobiol. 2004;80:525-30 pubmed
    ..The FR-induced B band showed an upshift after freezing. These results suggest that AG is a normal component of TL bands induced not only by FR, but also by single turnover flash. ..
  8. Hegazy A, Yamada D, Kobayashi M, Kohno S, Ueno M, Ali M, et al. Therapeutic Strategy for Targeting Aggressive Malignant Gliomas by Disrupting Their Energy Balance. J Biol Chem. 2016;291:21496-21509 pubmed
    ..In human patient-derived glioma cells, nigericin, which reportedly suppresses cancer stem cell properties, induced AMPK phosphorylation that was associated with ..
  9. Allen A, Graham A. Mitochondrial function is involved in regulation of cholesterol efflux to apolipoprotein (apo)A-I from murine RAW 264.7 macrophages. Lipids Health Dis. 2012;11:169 pubmed publisher
    ..7 macrophages were treated with a range of concentrations of resveratrol, antimycin, dinitrophenol, nigericin and oligomycin, and changes in viability, cytotoxicity, membrane potential and ATP, compared with efflux of [3H]..
  10. Dupont N, Jiang S, Pilli M, Ornatowski W, Bhattacharya D, Deretic V. Autophagy-based unconventional secretory pathway for extracellular delivery of IL-1?. EMBO J. 2011;30:4701-11 pubmed publisher
    ..It enables a subset of cytosolic proteins devoid of signal peptide sequences, and thus unable to access the conventional pathway through the ER, to enter an autophagy-based secretory pathway facilitating their exit from the cytoplasm. ..
  11. Martinez Becerra P, Briz O, Romero M, Macias R, Perez M, Sancho Mateo C, et al. Further characterization of the electrogenicity and pH sensitivity of the human organic anion-transporting polypeptides OATP1B1 and OATP1B3. Mol Pharmacol. 2011;79:596-607 pubmed publisher
    ..substrate uptake in the presence of valinomycin (K(+)-ionophore), carbonyl cyanide 3-chlorophenylhydrazone and nigericin (protonophores), and amiloride (Na(+)/H(+)-inhibitor) and cation replacement in the medium were studied with ..
  12. Wei LaPierre L, Gong G, Gerstner B, Ducreux S, Yule D, Pouvreau S, et al. Respective contribution of mitochondrial superoxide and pH to mitochondria-targeted circularly permuted yellow fluorescent protein (mt-cpYFP) flash activity. J Biol Chem. 2013;288:10567-77 pubmed publisher
    ..However, matrix acidification modulated superoxide flash frequency in a bimodal manner. Low concentrations of nigericin (< 100 nM) that resulted in a mild dissipation of the mitochondrial pH gradient increased flash frequency, ..
  13. Won J, Park S, Hong S, Son S, Yu J. Rotenone-induced Impairment of Mitochondrial Electron Transport Chain Confers a Selective Priming Signal for NLRP3 Inflammasome Activation. J Biol Chem. 2015;290:27425-37 pubmed publisher
    ..transport chain by rotenone primes NLRP3 inflammasome activation only upon costimulation with ATP and not with nigericin or alum...
  14. Geng X, McDermott J, Lundgren J, Liu L, Tsai K, Shen J, et al. Role of AQP9 in transport of monomethyselenic acid and selenite. Biometals. 2017;30:747-755 pubmed publisher
    ..dissipate transmembrane proton potential or change the transmembrane pH gradient, such as FCCP, valinomycin and nigericin did not significantly inhibit MSeA uptake, suggesting MSeA transport is not proton coupled...
  15. Bond J, Varley J. Use of flow cytometry and SNARF to calibrate and measure intracellular pH in NS0 cells. Cytometry A. 2005;64:43-50 pubmed
    ..The first method uses nigericin to clamp pHi to external pH (pHe) and the second is the null point method...
  16. Sologub L, Kuehn A, Kern S, Przyborski J, Schillig R, Pradel G. Malaria proteases mediate inside-out egress of gametocytes from red blood cells following parasite transmission to the mosquito. Cell Microbiol. 2011;13:897-912 pubmed publisher
    ..PVM rupture can also be triggered by the ionophore nigericin and is sensitive to the cysteine protease inhibitor E-64d...
  17. Park E, Min K, Choi K, Kubatka P, Kruzliak P, Kim D, et al. Chloroquine enhances TRAIL-mediated apoptosis through up-regulation of DR5 by stabilization of mRNA and protein in cancer cells. Sci Rep. 2016;6:22921 pubmed publisher
    ..Other lysosomal inhibitors, including monensin and nigericin, also up-regulated DR5 and sensitized TRAIL-mediated apoptosis...
  18. Hansson M, Morota S, Teilum M, Mattiasson G, Uchino H, Elmér E. Increased potassium conductance of brain mitochondria induces resistance to permeability transition by enhancing matrix volume. J Biol Chem. 2010;285:741-50 pubmed publisher
    ..We propose that increased potassium conductance in brain mitochondria may cause a direct physiological effect on matrix volume inducing resistance to pathological calcium challenges. ..
  19. Sage S, Pugh N, Mason M, Harper A. Monitoring the intracellular store Ca2+ concentration in agonist-stimulated, intact human platelets by using Fluo-5N. J Thromb Haemost. 2011;9:540-51 pubmed publisher
    ..Evidence for a role for Ca(2+) -induced Ca(2+) release in agonist-evoked responses was obtained. Our data provide a validation of the use of Fluo-5N as a method for monitoring changes in [Ca(2+)](st) in human platelets. ..
  20. Chaddock A, Mant A, Karnauchov I, Brink S, Herrmann R, Klösgen R, et al. A new type of signal peptide: central role of a twin-arginine motif in transfer signals for the delta pH-dependent thylakoidal protein translocase. EMBO J. 1995;14:2715-22 pubmed
  21. Metelkin E, Demin O, Kovács Z, Chinopoulos C. Modeling of ATP-ADP steady-state exchange rate mediated by the adenine nucleotide translocase in isolated mitochondria. FEBS J. 2009;276:6942-55 pubmed publisher
    ..values of free matrix ATP and ADP, the model explains the diminished ADP-ATP exchange rate in the presence of nigericin, a condition in which there is hyperpolarization of the inner mitochondrial membrane at the expense of the ..
  22. Janneh O, Owen A, Bray P, Back D, Pirmohamed M. The accumulation and metabolism of zidovudine in 3T3-F442A pre-adipocytes. Br J Pharmacol. 2010;159:484-93 pubmed publisher
    ..Intracellular accumulation of ZDV was rapid, reaching equilibrium within 20 min; nigericin increased accumulation by 1.9-fold, but this did not alter the generation of ZDV mono-, di- and triphosphate...
  23. Gusev G, Ivanova T. Amiloride-sensitive sodium transport in lamprey red blood cells: evidence for two distinct transport pathways. Gen Physiol Biophys. 2004;23:443-56 pubmed
    ..0 using the K+/H+ ionophore nigericin. Incubation of acidified erythrocytes in a NaCl medium at pH 8...
  24. Ruiz F, Lea C, Oldfield E, Docampo R. Human platelet dense granules contain polyphosphate and are similar to acidocalcisomes of bacteria and unicellular eukaryotes. J Biol Chem. 2004;279:44250-7 pubmed
    ..This was indicated by the following: 1) the increase in [Ca2+]i induced by nigericin, monensin, or the weak base, NH4Cl, in the nominal absence of extracellular Ca2 and 2) the effect of ionomycin, ..
  25. Matsubara S, Chow W. Populations of photoinactivated photosystem II reaction centers characterized by chlorophyll a fluorescence lifetime in vivo. Proc Natl Acad Sci U S A. 2004;101:18234-9 pubmed
    ..Acceleration of photoinactivation of PSII by a pretreatment with the inhibitors/uncoupler lincomycin, DTT, or nigericin further reduced PSII Chl a fluorescence lifetimes...
  26. Mendoza M, Mijares A, Rojas H, Rodriguez J, Urbina J, Dipolo R. Physiological and morphological evidences for the presence acidocalcisomes in Trypanosoma evansi: single cell fluorescence and 31P NMR studies. Mol Biochem Parasitol. 2002;125:23-33 pubmed
    ..It has been characterized physiologically as a Ca(2+) store sensitive to nigericin. The Ca(2+)/H(+)-ATPase is the system responsible for Ca(2+) accumulation, which depends on a pH gradient formed ..
  27. Telfer A, Barber J. Twofold effect of valinomycin on isolated spinach chloroplasts: uncoupling and inhibition of electron transport. Biochim Biophys Acta. 1974;333:343-52 pubmed
    ..above 10(-6) M inhibits electron flow and stimulates chlorophyll a fluorescence of chloroplasts uncoupled by nigericin or NH4Cl...
  28. Brake J, Cummings T, Pinedo C, Bafundo K. Effects of the ionophore anticoccidial semduramicin on broiler breeders. Poult Sci. 2001;80:260-5 pubmed
    ..The data show that adverse effects of semduramicin require greater than 1 wk of exposure to be evident. ..
  29. Bissinger R, Malik A, Bouguerra G, Zhou Y, Singh Y, Abbès S, et al. Triggering of Suicidal Erythrocyte Death by the Antibiotic Ionophore Nigericin. Basic Clin Pharmacol Toxicol. 2016;118:381-9 pubmed publisher
    The K(+),H(+) ionophore and antibiotic nigericin has been shown to trigger apoptosis and is thus considered for the treatment of malignancy...
  30. Lucas C, Gillies R, Olson J, Giuliano K, Martinez R, Sneider J. Intracellular acidification inhibits the proliferative response in BALB/c-3T3 cells. J Cell Physiol. 1988;136:161-7 pubmed
    ..These observations suggest that cytoplasmic acidification may inhibit proliferation through its effects on protein synthesis. ..
  31. Nakai Y, Inoue K, Abe N, Hatakeyama M, Ohta K, Otagiri M, et al. Functional characterization of human proton-coupled folate transporter/heme carrier protein 1 heterologously expressed in mammalian cells as a folate transporter. J Pharmacol Exp Ther. 2007;322:469-76 pubmed
    ..We also found that sulfasalazine is a potent inhibitor of hPCFT/HCP1, which would interfere with the intestinal absorption of MTX when coadministered in therapy for rheumatoid arthritis as well as folate. ..
  32. Ganas P, Mihasan M, Igloi G, Brandsch R. A two-component small multidrug resistance pump functions as a metabolic valve during nicotine catabolism by Arthrobacter nicotinovorans. Microbiology. 2007;153:1546-55 pubmed publisher
    ..inhibitors 2,4-dinitrophenol (DNP), carbonyl cyanide m-chlorophenylhydrazone (CCCP) or the proton ionophore nigericin, export of methylamine was inhibited and radioactivity accumulated inside the bacteria...
  33. Serrano M, Wang B, Aryal B, Garcion C, Abou Mansour E, Heck S, et al. Export of salicylic acid from the chloroplast requires the multidrug and toxin extrusion-like transporter EDS5. Plant Physiol. 2013;162:1815-21 pubmed publisher
    ..The location at the chloroplast envelope supports a model of the effect of EDS5 on SA biosynthesis: in the eds5 mutant, stress-induced SA is trapped in the chloroplast and inhibits its own accumulation by autoinhibitory feedback. ..
  34. Boesch M, Zeimet A, Rumpold H, Gastl G, Sopper S, Wolf D. Drug Transporter-Mediated Protection of Cancer Stem Cells From Ionophore Antibiotics. Stem Cells Transl Med. 2015;4:1028-32 pubmed publisher
    ..transporters are involved in protection from the cytotoxic effects of the ionophore antibiotics salinomycin and nigericin. Our experiments demonstrated that ionophore antibiotics were ineffective against both stem-like ovarian cancer ..
  35. Evans E, Crofts A. The relationship between delayed fluorescence and the carotenoid shift in chromatophores from Rhodopseudomonas capsulata. Biochim Biophys Acta. 1974;333:44-51 pubmed
    ..2) The antibiotics valinomycin, nigericin and antimycin, showed qualitatively similar effects on delayed fluorescence and the carotenoid shift, but the ..
  36. Kuida K, Lippke J, Ku G, Harding M, Livingston D, Su M, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme. Science. 1995;267:2000-3 pubmed
    ..Despite this defect in apoptosis, ICE-/- mice proceed normally through development. ..
  37. Chen C, Chen S. Lactate transport and glycolytic activity in the freshly isolated rabbit cornea. Arch Biochem Biophys. 1990;276:70-6 pubmed
    ..These findings suggest that the ionophore-mediated lactate efflux favors the reduction of low pyruvate concentration in the tissue, rather than parallel increases in glycolytic activity. ..
  38. McDermott J, Rosen B, Liu Z. Jen1p: a high affinity selenite transporter in yeast. Mol Biol Cell. 2010;21:3934-41 pubmed publisher
    ..In addition, selenite and lactate can inhibit the transport of each other competitively. Therefore, we postulate selenite is a molecular mimic of monocarboxylates which allows selenite to be transported by Jen1p. ..
  39. Chaidee A, Wongchai C, Pfeiffer W. Extracellular alkaline phosphatase is a sensitive marker for cellular stimulation and exocytosis in heterotroph cell cultures of Chenopodium rubrum. J Plant Physiol. 2008;165:1655-66 pubmed publisher
    ..as a marker for cellular stimulation by its high correlations with induced changes of extracellular pH: 10microM nigericin (correlation coefficient r=0.91), 100microM salicylic acid (r=0.84), heat shock 5min 37 degrees C (r=0...
  40. Chung H, Vilaysane A, Lau A, Stahl M, Morampudi V, Bondzi Simpson A, et al. NLRP3 regulates a non-canonical platform for caspase-8 activation during epithelial cell apoptosis. Cell Death Differ. 2016;23:1331-46 pubmed publisher
    ..caspase-1, or IL-1β secretion in response to TNFα/CHX or NLRP3-dependent triggers, such as ATP or nigericin. In cell fractionation studies and by confocal microscopy, NLRP3 colocalized with ASC and caspase-8 in speck-..
  41. Hwang I, Lee E, Jeon S, Yu J. Histone deacetylase 6 negatively regulates NLRP3 inflammasome activation. Biochem Biophys Res Commun. 2015;467:973-8 pubmed publisher
    ..In addition, knockdown of HDAC6 in BMDMs enhanced the oligomerization of ASC upon LPS/nigericin stimulation...
  42. Akhmedov D, Braun M, Mataki C, Park K, Pozzan T, Schoonjans K, et al. Mitochondrial matrix pH controls oxidative phosphorylation and metabolism-secretion coupling in INS-1E clonal beta cells. FASEB J. 2010;24:4613-26 pubmed publisher
    ..Acidification of the mitochondrial matrix pH by nigericin blunted nutrient-dependent respiratory and ATP responses (continuously monitored in intact cells)...
  43. Kummari E, Alugubelly N, Hsu C, Dong B, Nanduri B, Edelmann M. Activity-Based Proteomic Profiling of Deubiquitinating Enzymes in Salmonella-Infected Macrophages Leads to Identification of Putative Function of UCH-L5 in Inflammasome Regulation. PLoS ONE. 2015;10:e0135531 pubmed publisher
    ..UCH-L5 activity, or in UCH-L5 protein amount in chicken and human macrophages infected or stimulated with LPS/nigericin, led to decreased IL-1β release...
  44. Le Petit Thevenin J, Nobili O, Verine A, Boyer J. Selective modulation of membrane sphingomyelin fatty acid turnover by nigericin. A study in the rat reticulocyte. Life Sci. 1996;59:PL289-94 pubmed
    Exposure of rat reticulocytes to Nigericin produced a selective modulation of fatty acid incorporation into sphingomyelin (SM) of the cell membrane, via changes in SM acylation kinetics...
  45. Schubert R, Krien U, Gagov H. Protons inhibit the BK(Ca) channel of rat small artery smooth muscle cells. J Vasc Res. 2001;38:30-8 pubmed
  46. Maher S, Basit H, Forster R, Keyes T. Micron dimensioned cavity array supported lipid bilayers for the electrochemical investigation of ionophore activity. Bioelectrochemistry. 2016;112:16-23 pubmed publisher
    ..Two ionophores were studied at the MSLBs; Valinomycin, a K(+) uniporter and Nigericin, a K(+)/H(+) antiporter...
  47. Kuivaniemi H, Ala Kokko L, Kivirikko K. Secretion of lysyl oxidase by cultured human skin fibroblasts and effects of monensin, nigericin, tunicamycin and colchicine. Biochim Biophys Acta. 1986;883:326-34 pubmed
    ..Culturing of the cells for 8 h in the presence of either monensin or nigericin, ionophores known to inhibit the secretion of many proteins at the level of the Golgi complex, markedly reduced ..
  48. Orlowski G, Sharma S, Colbert J, Bogyo M, Robertson S, Kataoka H, et al. Frontline Science: Multiple cathepsins promote inflammasome-independent, particle-induced cell death during NLRP3-dependent IL-1β activation. J Leukoc Biol. 2017;102:7-17 pubmed publisher
    ..In contrast, nigericin, a soluble activator of NLRP3 inflammasomes, induced cell death that was dependent on the NLRP3...
  49. Kong X, Su F, Zhang L, Yaron J, Lee F, Shi Z, et al. A highly selective mitochondria-targeting fluorescent K(+) sensor. Angew Chem Int Ed Engl. 2015;54:12053-7 pubmed publisher
    ..K(+) efflux/influx in the mitochondria was observed upon stimulation with ionophores, nigericin, or ionomycin...
  50. Martins de Oliveira R, Antunes E, Pedrazzoli J, Gambero A. The inhibitory effects of H+ K+ ATPase inhibitors on human neutrophils in vitro: restoration by a K+ ionophore. Inflamm Res. 2007;56:105-11 pubmed
    ..Omeprazole was also able to decrease intracellular calcium availability. The addition of a potassium ionophore, nigericin, restored the migratory ability, as well as the intracellular calcium levels...
  51. Ha J, Song G, Ai L, Li J. Determination of six polyether antibiotic residues in foods of animal origin by solid phase extraction combined with liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2016;1017-1018:187-194 pubmed publisher
    ..determination of six polyether antibiotics, including lasalocid, salinomycin, monensin, narasin, madubamycin and nigericin residues, in foods of animal origin...
  52. Shi H, Murray A, Beutler B. Reconstruction of the Mouse Inflammasome System in HEK293T Cells. Bio Protoc. 2016;6: pubmed publisher
    ..Here, we describe how to co-transfect the NLRP3 inflammasome components into HEK293T cells, which enables inflammasome activation and the production of IL-1? upon stimulation with nigericin.
  53. Miyake C, Yonekura K, Kobayashi Y, Yokota A. Cyclic electron flow within PSII functions in intact chloroplasts from spinach leaves. Plant Cell Physiol. 2002;43:951-7 pubmed
    ..The addition of the protonophore nigericin to the intact chloroplasts decreased Phi(PSII), but increased V(O(2)), and relative electron flux in PSII [Phi(..