firefly luciferin


Summary: A benzothaizole which is oxidized by LUCIFERASES, FIREFLY to cause emission of light (LUMINESCENCE).

Top Publications

  1. Branchini B, Southworth T, Murtiashaw M, Magyar R, Gonzalez S, Ruggiero M, et al. An alternative mechanism of bioluminescence color determination in firefly luciferase. Biochemistry. 2004;43:7255-62 pubmed
    ..Ruggiero, M. C., and Stroh, J. G. (2002) J. Am. Chem. Soc. 124, 2112-2113) that the synthetic adenylate of firefly luciferin analogue D-5,5-dimethylluciferin was transformed into the emitter 5,5-dimethyloxyluciferin in ..
  2. Gross S, Abraham U, Prior J, Herzog E, Piwnica Worms D. Continuous delivery of D-luciferin by implanted micro-osmotic pumps enables true real-time bioluminescence imaging of luciferase activity in vivo. Mol Imaging. 2007;6:121-30 pubmed
    ..We conclude that implanted pumps provide reliable, prolonged substrate delivery for high temporal resolution BLI, traversing complications of repetitive substrate injections. ..
  3. Honigman A, Zeira E, Ohana P, Abramovitz R, Tavor E, Bar I, et al. Imaging transgene expression in live animals. Mol Ther. 2001;4:239-49 pubmed
    ..We showed that the CCCD photon detection system is a simple, reproducible, and applicable method that enables the continuous monitoring of a gene delivery system in living animals. ..
  4. Theodossiou T, Hothersall J, Woods E, Okkenhaug K, Jacobson J, MacRobert A. Firefly luciferin-activated rose bengal: in vitro photodynamic therapy by intracellular chemiluminescence in transgenic NIH 3T3 cells. Cancer Res. 2003;63:1818-21 pubmed
    ..Here we show the in vitro photodynamic effect of rose bengal activated by intracellular generation of light, in luciferase-transfected NIH 3T3 murine fibroblasts. ..
  5. Presiado I, Erez Y, Huppert D. Excited-state intermolecular proton transfer of firefly luciferin III. Proton transfer to a mild base. J Phys Chem A. 2010;114:13337-46 pubmed publisher
    ..The decay rate of the third, long-time component is proportional to the acetate concentration. We attribute it to the diffusion-assisted reaction as well as to PT process to the solvent. ..
  6. Franks N, Jenkins A, Conti E, Lieb W, Brick P. Structural basis for the inhibition of firefly luciferase by a general anesthetic. Biophys J. 1998;75:2205-11 pubmed
    ..2-A resolution. These results provide a structural basis for understanding the anesthetic inhibition of the enzyme, as well as an explanation for the ATP modulation of its anesthetic sensitivity. ..
  7. Li J, Holman D, Li H, Liu A, Beres B, Hankins G, et al. Long-term tracing of adenoviral expression in rat and rabbit using luciferase imaging. J Gene Med. 2005;7:792-802 pubmed
    ..Live imaging of luciferase expression can be performed not only in small animals, but also in larger animals such as rabbits. ..
  8. Pillon A, Servant N, Vignon F, Balaguer P, Nicolas J. In vivo bioluminescence imaging to evaluate estrogenic activities of endocrine disrupters. Anal Biochem. 2005;340:295-302 pubmed
    ..Different administration protocols have been used and good correlation was observed for most products. However, we found that ethynylestradiol was the most efficient chemical when orally administered. ..
  9. Woodroofe C, Meisenheimer P, Klaubert D, Kovic Y, Rosenberg J, Behney C, et al. Novel heterocyclic analogues of firefly luciferin. Biochemistry. 2012;51:9807-13 pubmed publisher
    Five novel firefly luciferin analogues in which the benzothiazole ring system of the natural substrate was replaced with benzimidazole, benzofuran, benzothiophene, benzoxazole, and indole were synthesized...

More Information

Publications107 found, 100 shown here

  1. Raha S, Myint A, Johnstone L, Robinson B. Control of oxygen free radical formation from mitochondrial complex I: roles for protein kinase A and pyruvate dehydrogenase kinase. Free Radic Biol Med. 2002;32:421-30 pubmed
    ..In light of these observations we discuss a potential model for the dual regulation of complex I and the production of oxygen free radicals by both PKA and PDH kinase. ..
  2. Kondo Y, Ishigami A, Kubo S, Handa S, Gomi K, Hirokawa K, et al. Senescence marker protein-30 is a unique enzyme that hydrolyzes diisopropyl phosphorofluoridate in the liver. FEBS Lett. 2004;570:57-62 pubmed
    ..Consequently, a reduction of SMP30 expression might account for the age-associated deterioration of cellular functions and enhanced susceptibility to harmful stimuli in aged tissue. ..
  3. Selloum L, Reichl S, Muller M, Sebihi L, Arnhold J. Effects of flavonols on the generation of superoxide anion radicals by xanthine oxidase and stimulated neutrophils. Arch Biochem Biophys. 2001;395:49-56 pubmed
    ..Thus, an inhibition of enzymes involved in signaling rather than a scavenging of superoxide anion radicals dominates in fMet-Leu-Phe-stimulated neutrophils exposed to flavonols. ..
  4. Orlova G, Goddard J, Brovko L. Theoretical study of the amazing firefly bioluminescence: the formation and structures of the light emitters. J Am Chem Soc. 2003;125:6962-71 pubmed
    ..According to predictions at the TDDFT B3LYP level, the color of the bioluminescence depends on the polarization of the OxyLH(2) in the microenvironment of the enzyme-OxyLH(2) complex. ..
  5. Akimoto H, Wu C, Kinumi T, Ohmiya Y. Biological rhythmicity in expressed proteins of the marine dinoflagellate Lingulodinium polyedrum demonstrated by chronological proteomics. Biochem Biophys Res Commun. 2004;315:306-12 pubmed
    ..Thus, rhythmicity appears to be related to physiological states and to control the metabolic pathway in L. polyedrum. ..
  6. Niwa K, Nakamura M, Ohmiya Y. Stereoisomeric bio-inversion key to biosynthesis of firefly D-luciferin. FEBS Lett. 2006;580:5283-7 pubmed
    ..We conclude that the selection of chirality in living organisms is not as rigid as previously thought. ..
  7. Reichl S, Vocks A, Petkovic M, Schiller J, Arnhold J. The photoprotein pholasin as a luminescence substrate for detection of superoxide anion radicals and myeloperoxidase activity in stimulated neutrophils. Free Radic Res. 2001;35:723-33 pubmed
    ..Thus, Pholasin luminescence can be used to detect, sensitively and specifically, O2- production and MPO release from stimulated neutrophils. ..
  8. Rodionova N, Bondar V, Petushkov V. ATP is a cosubstrate of the luciferase of the earthworm Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae). Dokl Biochem Biophys. 2003;392:253-5 pubmed
  9. Cazzonelli C, Burke J, Velten J. Functional characterization of the geminiviral conserved late element (CLE) in uninfected tobacco. Plant Mol Biol. 2005;58:465-81 pubmed
    ..These data suggests that the PHV-AC2 protein, instead of interacting directly with CLE, functions as either a general transcriptional activator and/or a suppressor of post-transcriptional gene silencing. ..
  10. Taguchi T, Kozaki Y, Katanosaka K, Mizumura K. Compression-induced ATP release from rat skeletal muscle with and without lengthening contraction. Neurosci Lett. 2008;434:277-81 pubmed publisher
    ..The amount of ATP released from the muscle preparations was not different between the non-treated control and the LC group. These results provide clear evidence that ATP is released from rat skeletal muscle by compression. ..
  11. Ioka S, Saitoh T, Iwano S, Suzuki K, Maki S, Miyawaki A, et al. Synthesis of Firefly Luciferin Analogues and Evaluation of the Luminescent Properties. Chemistry. 2016;22:9330-7 pubmed publisher
    Five new firefly luciferin (1) analogues were synthesized and their light emission properties were examined...
  12. Goldenberg S, Veriabo N, Soslau G. A micromethod to measure platelet aggregation and atp release by impedance. Thromb Res. 2001;103:57-61 pubmed
    ..ATP release was recorded concurrently with aggregation by measuring the luminescence from the firefly luciferin-luciferase reaction...
  13. Gomi K, Kajiyama N. Oxyluciferin, a luminescence product of firefly luciferase, is enzymatically regenerated into luciferin. J Biol Chem. 2001;276:36508-13 pubmed
    ..pyralis deduced from the nucleotide sequence was shown to consist of 308 amino acids with a molecular weight of 33,619. The cDNA was successfully expressed under the control of the tac promoter in Escherichia coli. ..
  14. Zhou G, Kamahori M, Okano K, Chuan G, Harada K, Kambara H. Quantitative detection of single nucleotide polymorphisms for a pooled sample by a bioluminometric assay coupled with modified primer extension reactions (BAMPER). Nucleic Acids Res. 2001;29:E93 pubmed
    ..The method is sensitive enough to detect 14 amol ssM13 DNA. The proposed method seems very promising in terms of realizing a cost-effective, large-scale human genetic testing system. ..
  15. Jawhara S, Mordon S. Monitoring of bactericidal action of laser by in vivo imaging of bioluminescent E. coli in a cutaneous wound infection. Lasers Med Sci. 2006;21:153-9 pubmed
    ..However, the exact mechanism deserves further studies. This study points out the advantage of using bioluminescence imaging to evaluate laser for the treatment of acute infections in vivo, nondestructively, and noninvasively. ..
  16. Rowe L, Dikici E, Daunert S. Engineering bioluminescent proteins: expanding their analytical potential. Anal Chem. 2009;81:8662-8 pubmed publisher
    ..To listen to a podcast about this feature, please go to the Analytical Chemistry multimedia page at .). ..
  17. Näslund B, Stahle L, Lundin A, Anderstam B, Arner P, Bergstrom J. Luminometric single step urea assay using ATP-hydrolyzing urease. Clin Chem. 1998;44:1964-73 pubmed
    ..The results obtained with the present assay were highly correlated for dialysate (r = 0.979) and for plasma (r = 0.978) with those obtained by a spectrophotometric kit method with slopes of 1.02-1.03 and intercepts of 0.08-0.23 mmol/L. ..
  18. Cui K, Xu X, Zhao H, Wong S. A quantitative study of factors affecting in vivo bioluminescence imaging. Luminescence. 2008;23:292-5 pubmed publisher
    ..The data indicate that a carefully designed BLI experiment is required to generate optimal and consistent results. ..
  19. Cresce A, Dandu R, Burger A, Cappello J, Ghandehari H. Characterization and real-time imaging of gene expression of adenovirus embedded silk-elastinlike protein polymer hydrogels. Mol Pharm. 2008;5:891-7 pubmed publisher
    ..The potential to modulate viral release using SELP hydrogel delivery vehicles that can be injected intratumorally by minimally invasive techniques holds significant promise for the delivery of therapeutic viruses. ..
  20. Takakura H, Sasakura K, Ueno T, Urano Y, Terai T, Hanaoka K, et al. Development of luciferin analogues bearing an amino group and their application as BRET donors. Chem Asian J. 2010;5:2053-61 pubmed publisher
    ..Stable bioluminescence that is not influenced by environmental factors is highly desirable for reliable measurements in biological assays. ..
  21. da Silva L, da Silva J. Kinetics of inhibition of firefly luciferase by dehydroluciferyl-coenzyme A, dehydroluciferin and L-luciferin. Photochem Photobiol Sci. 2011;10:1039-45 pubmed publisher
    ..L-CoA K(i) supports the conclusion that CoA can stimulate the light emission reaction by provoking the formation of a weaker inhibitor. ..
  22. Buckle T, van Leeuwen F. Validation of intratracheal instillation of lung tumour cells in mice using single photon emission computed tomography/computed tomography imaging. Lab Anim. 2010;44:40-5 pubmed publisher
    ..The reported transplantation procedure enables the assessment of injection accuracy and provides a good approach for the generation of orthotopic lung tumour models for future response imaging studies. ..
  23. Van de Bittner G, Dubikovskaya E, Bertozzi C, Chang C. In vivo imaging of hydrogen peroxide production in a murine tumor model with a chemoselective bioluminescent reporter. Proc Natl Acad Sci U S A. 2010;107:21316-21 pubmed publisher
    ..PCL-1 is a boronic acid-caged firefly luciferin molecule that selectively reacts with H(2)O(2) to release firefly luciferin, which triggers a bioluminescent ..
  24. Swindle E, Hunt J, Coleman J. A comparison of reactive oxygen species generation by rat peritoneal macrophages and mast cells using the highly sensitive real-time chemiluminescent probe pholasin: inhibition of antigen-induced mast cell degranulation by macrophage-derived hydrogen. J Immunol. 2002;169:5866-73 pubmed
    ..The latter could be an important mechanism whereby phagocytic cells regulate mast cell activation and promote resolution of IgE-mediated inflammation. ..
  25. Oba Y, Shintani T, Nakamura T, Ojika M, Inouye S. Determination of the luciferin contents in luminous and non-luminous beetles. Biosci Biotechnol Biochem. 2008;72:1384-7 pubmed
    The contents of firefly luciferin in luminous and non-luminous beetles were determined by the methods of HPLC with fluorescence detection and the luminescence reaction of luciferin and firefly luciferase...
  26. Oba Y, Furuhashi M, Inouye S. Identification of a functional luciferase gene in the non-luminous diurnal firefly, Lucidina biplagiata. Insect Mol Biol. 2010;19:737-43 pubmed publisher
    ..cruciata. As previously reported, the content of luciferin in L. biplagiata was less than 0.1% of that in L. cruciata. Thus, the non-luminosity of L. biplagiata might be explained by low levels of both luciferase and luciferin. ..
  27. Wu W, Chen L, Li J, Du L, Li M. Bioluminogenic Imaging of AminopeptidaseN In Vitro and In Vivo. Methods Mol Biol. 2016;1461:91-9 pubmed publisher
    ..As one of the most popular bioluminescence system, the firefly luciferin-luciferase system is exceptionally suitable for deep tissue imaging in living animals owing to its long ..
  28. White E, Rapaport E, Hopkins T, Seliger H. Chemi- and bioluminescence of firefly luciferin. J Am Chem Soc. 1969;91:2178-80 pubmed
  29. Gomi K, Hirokawa K, Kajiyama N. Molecular cloning and expression of the cDNAs encoding luciferin-regenerating enzyme from Luciola cruciata and Luciola lateralis. Gene. 2002;294:157-66 pubmed
    ..G-LRE and H-LRE were successfully expressed under the control of the lac promoter in Escherichia coli. ..
  30. Bhaumik S, Lewis X, Gambhir S. Optical imaging of Renilla luciferase, synthetic Renilla luciferase, and firefly luciferase reporter gene expression in living mice. J Biomed Opt. 2004;9:578-86 pubmed
    ..These studies demonstrate that hRluc should be a useful primary reporter gene with high sensitivity when used alone or in conjunction with other bioluminescence reporter genes for imaging in living rodents. ..
  31. Takahashi H, Nishina A, Fukumoto R, Kimura H, Koketsu M, Ishihara H. Selenoureas and thioureas are effective superoxide radical scavengers in vitro. Life Sci. 2005;76:2185-92 pubmed
    ..A possibility that selenoureas could use it as a new superoxide anion-scavenging substance from the result of this research became clear. ..
  32. Glebska J, Koppenol W. Chemiluminescence of Pholasin caused by peroxynitrite. Free Radic Biol Med. 2005;38:1014-22 pubmed
    ..However, many questions concerning the mechanism of the light emission remain to be elucidated. ..
  33. Garcia M, Ma D, Dicioccio A, Cali J. The use of a high-throughput luminescent method to assess CYP3A enzyme induction in cultured rat hepatocytes. In Vitro Cell Dev Biol Anim. 2008;44:129-34 pubmed publisher
  34. Jones K, Hibbert F, Keenan M. Glowing jellyfish, luminescence and a molecule called coelenterazine. Trends Biotechnol. 1999;17:477-81 pubmed
    ..The luminescent system of the jellyfish Aequorea victoria consists of the photoprotein aequorin, which contains the molecule coelenterazine as a prosthetic group and shows considerable potential in this area. ..
  35. Branchini B, Southworth T, Khattak N, Michelini E, Roda A. Red- and green-emitting firefly luciferase mutants for bioluminescent reporter applications. Anal Biochem. 2005;345:140-8 pubmed
    ..The results presented here with the P. pyralis mutants provide evidence that simultaneous multiple analyte assay development is feasible with these novel proteins that require only a single substrate. ..
  36. Nakata N, Kamidate T. Enhanced firefly bioluminescent assay of adenosine 5'-triphosphate using liposomes containing cationic cholesterols. Luminescence. 2001;16:263-9 pubmed
    ..0 pmol/L in an aqueous standard solution. The BL enhancement in the presence of cationic liposomes could be explained in terms of BL emitters and electrostatic interaction between the liposomes surface and BL reactants. ..
  37. Haddock S, Rivers T, Robison B. Can coelenterates make coelenterazine? Dietary requirement for luciferin in cnidarian bioluminescence. Proc Natl Acad Sci U S A. 2001;98:11148-51 pubmed
    ..This evidence regarding the origins of luciferin in Cnidaria has implications for the evolution of bioluminescence and for the extensive use of coelenterazine among marine organisms. ..
  38. Yang N, Ho W, Chen Y, Hu M. A convenient one-step extraction of cellular ATP using boiling water for the luciferin-luciferase assay of ATP. Anal Biochem. 2002;306:323-7 pubmed
    ..Thus, this one-step method is suitable for routine assay of cellular ATP for both suspended and attached cells. ..
  39. Godinat A, Park H, Miller S, Cheng K, Hanahan D, Sanman L, et al. A biocompatible in vivo ligation reaction and its application for noninvasive bioluminescent imaging of protease activity in living mice. ACS Chem Biol. 2013;8:987-99 pubmed publisher
    ..Lastly, the luciferin ligation reaction is 3 orders of magnitude faster than Staudinger ligation, suggesting further applications for both bioluminescence and specific molecular targeting in vivo. ..
  40. Sato K, Sato N, Shimizu H, Tsutiya T, Takahashi H, Kakizaki S, et al. Faropenem enhances superoxide anion production by human neutrophils in vitro. J Antimicrob Chemother. 1999;44:337-41 pubmed
    ..Faropenem might be potentially advantageous in the treatment of infections because a synergic interaction of antibodies and cytocidal neutrophils is necessary for the early eradication of the pathogenic bacteria. ..
  41. Fraga H, Esteves da Silva J, Fontes R. Identification of luciferyl adenylate and luciferyl coenzyme a synthesized by firefly luciferase. Chembiochem. 2004;5:110-5 pubmed
    ..The luciferase-catalyzed synthesis of these coenzyme A derivatives may be a consequence of the postulated recent evolutionary origin of firefly luciferases from an ancestral acyl-coenzyme A synthetase. ..
  42. Shenker N, Cohen H, Blake D. Developing concepts in allodynic pain. Clin Med (Lond). 2008;8:79-82 pubmed
  43. Dragulescu Andrasi A, Liang G, Rao J. In vivo bioluminescence imaging of furin activity in breast cancer cells using bioluminogenic substrates. Bioconjug Chem. 2009;20:1660-6 pubmed publisher
    ..Direct imaging of furin activity may facilitate the study of furin function in tumorigenicity and the discovery of new drugs for furin-targeted cancer therapy. ..
  44. Younes A, Lukyanenko Y, Lyashkov A, Lakatta E, Sollott S. A bioluminescence method for direct measurement of phosphodiesterase activity. Anal Biochem. 2011;417:36-40 pubmed publisher
    ..This single-step enzyme- and substrate-coupled cyclic-reaction system yields a simplified, sensitive, reproducible, and accurate method for quantifying PDE activities in small biological samples. ..
  45. Pesnel S, Pillon A, Creancier L, Guilbaud N, Bailly C, Kruczynski A, et al. Quantitation in bioluminescence imaging by correction of tissue absorption for experimental oncology. Mol Imaging Biol. 2011;13:646-52 pubmed publisher
    ..Spectral analysis allows improved quantitation of BLI and could be of value to perform pharmacological studies and to follow tumour progression in models with tumours evolving in different locations. ..
  46. Craig F, Simmonds A, Watmore D, McCapra F, White M. Membrane-permeable luciferin esters for assay of firefly luciferase in live intact cells. Biochem J. 1991;276 ( Pt 3):637-41 pubmed
    ..Alternative luciferin derivatives may allow further improvements in sensitivity. ..
  47. Greenman L, Mazziotti D. Balancing single- and multi-reference correlation in the chemiluminescent reaction of dioxetanone using the anti-Hermitian contracted Schrödinger equation. J Chem Phys. 2011;134:174110 pubmed publisher
    ..Dioxetanone, the light-producing moiety of firefly luciferin, efficiently converts chemical energy into light by accessing its excited-state surface via a conical ..
  48. Minekawa T, Ohkuma H, Abe K, Maekawa H, Arakawa H. Practical application of bioluminescence enzyme immunoassay using enhancer for firefly luciferin-luciferase bioluminescence. Luminescence. 2011;26:167-71 pubmed publisher
    b>Firefly luciferin-luciferase bioluminescence is known for its high quantum yield (41.0 ± 7.4%). Given this high quantum yield, application of this bioluminescence is expected to be useful in the field of clinical diagnostics...
  49. Doshi U, Li A. Luciferin IPA-based higher throughput human hepatocyte screening assays for CYP3A4 inhibition and induction. J Biomol Screen. 2011;16:903-9 pubmed publisher
  50. Dubuisson M, Marchand C, Rees J. Fire fly luciferin as antioxidant and light emitter: the evolution of insect bioluminescence. Luminescence. 2004;19:339-44 pubmed
  51. Bloquel C, Trollet C, Pradines E, Seguin J, Scherman D, Bureau M. Optical imaging of luminescence for in vivo quantification of gene electrotransfer in mouse muscle and knee. BMC Biotechnol. 2006;6:16 pubmed
  52. Roncoroni C, Rizzi N, Brunialti E, Cali J, Klaubert D, Maggi A, et al. Molecular imaging of cytochrome P450 activity in mice. Pharmacol Res. 2012;65:531-6 pubmed publisher
    ..The kind of tool generated has the potential to be exploited for the study of additional CYPs. ..
  53. Hirano T, Nagai H, Matsuhashi T, Hasumi Y, Iwano S, Ito K, et al. Spectroscopic studies of the color modulation mechanism of firefly (beetle) bioluminescence with amino-analogs of luciferin and oxyluciferin. Photochem Photobiol Sci. 2012;11:1281-4 pubmed publisher
    ..Fluorescence solvatochromic character of aminooxyluciferin analogs indicates that the bioluminescence of aminoluciferin is useful for evaluating the polarity of a luciferase active site. ..
  54. Pinto da Silva L, Santos A, Esteves da Silva J. Efficient firefly chemi/bioluminescence: evidence for chemiexcitation resulting from the decomposition of a neutral firefly dioxetanone molecule. J Phys Chem A. 2013;117:94-100 pubmed publisher
    ..It was also studied for the first time the interaction between a molecule involved in the bioluminescence reaction (neutral firefly dioxetanone) and the real second conformation of firefly luciferase. ..
  55. Tucker T, Varga K, Bebok Z, Zsembery A, McCarty N, Collawn J, et al. Transient transfection of polarized epithelial monolayers with CFTR and reporter genes using efficacious lipids. Am J Physiol Cell Physiol. 2003;284:C791-804 pubmed
    ..Transient transfection of epithelial monolayers provides a powerful system in which to express the cDNA of any epithelium-specific protein transiently in a native polarized epithelium to study protein function. ..
  56. Branchini B, Murtiashaw M, Carmody J, Mygatt E, Southworth T. Synthesis of an N-acyl sulfamate analog of luciferyl-AMP: a stable and potent inhibitor of firefly luciferase. Bioorg Med Chem Lett. 2005;15:3860-4 pubmed
    ..We anticipate that the novel N-acyl sulfamate analog will be a valuable reagent in future studies designed to elucidate the role of conformational changes in firefly luciferase catalyzed bioluminescence. ..
  57. Ristenpart W, Wan J, Stone H. Enzymatic reactions in microfluidic devices: Michaelis-Menten kinetics. Anal Chem. 2008;80:3270-6 pubmed publisher
    ..We corroborate the x5/2 scaling result experimentally using the bioluminescent reaction between ATP and luciferase/luciferin as a model system. ..
  58. Eiríksdóttir E, Mäger I, Lehto T, El Andaloussi S, Langel U. Cellular internalization kinetics of (luciferin-)cell-penetrating peptide conjugates. Bioconjug Chem. 2010;21:1662-72 pubmed publisher
  59. Vieira J, Pinto da Silva L, Esteves da Silva J. Advances in the knowledge of light emission by firefly luciferin and oxyluciferin. J Photochem Photobiol B. 2012;117:33-9 pubmed publisher
    ..The objective of this manuscript is to review the experimental studies of firefly luciferin and oxyluciferin, and related analogues, fluorescence and bioluminescence.
  60. Robison B, Reisenbichler K, Hunt J, Haddock S. Light production by the arm tips of the deep-sea cephalopod Vampyroteuthis infernalis. Biol Bull. 2003;205:102-9 pubmed
    ..This paper presents observations on the structure and operation of the arm-tip light organs, the character of the luminous cloud, and how the light they produce is incorporated into behavioral patterns. ..
  61. Ohtani N, Imamura Y, Yamakoshi K, Hirota F, Nakayama R, Kubo Y, et al. Visualizing the dynamics of p21(Waf1/Cip1) cyclin-dependent kinase inhibitor expression in living animals. Proc Natl Acad Sci U S A. 2007;104:15034-9 pubmed
    ..These data illustrate the unique utility of bioluminescence imaging in advancing our understanding of the timing and, hence, likely roles of specific gene expression in higher eukaryotes. ..
  62. Marques S, da Silva J. An optimized luciferase bioluminescent assay for coenzyme A. Anal Bioanal Chem. 2008;391:2161-8 pubmed publisher
    ..80 microM. The assay has a relative standard deviation of about 7%. By coupling this optimized procedure to bioluminescent detection, a sensible and robust method can be obtained for the analysis of CoA. ..
  63. Sadikot R, Blackwell T. Bioluminescence: imaging modality for in vitro and in vivo gene expression. Methods Mol Biol. 2008;477:383-94 pubmed publisher
  64. Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, et al. Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin. Cell Death Differ. 2010;17:1003-10 pubmed publisher
  65. Shanmugasundararaj S, Lehle S, Yamodo H, Husain S, Tseng C, Nguyen K, et al. The location and nature of general anesthetic binding sites on the active conformation of firefly luciferase; a time resolved photolabeling study. PLoS ONE. 2012;7:e29854 pubmed publisher
    ..Second, a competitive one, in which anesthetics bind isosterically with luciferin. This work provides structural evidence that supports the competitive and allosteric actions previously characterized by kinetic studies. ..
  66. Yeh H, Karmach O, Ji A, Carter D, Martins Green M, Ai H. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. Nat Methods. 2017;14:971-974 pubmed publisher
    ..We adapted this pair to develop a bioluminescence resonance-energy-based Antares reporter called Antares2, which offers improved signal from deep tissues. ..
  67. Carlsen H, Moskaug J, Fromm S, Blomhoff R. In vivo imaging of NF-kappa B activity. J Immunol. 2002;168:1441-6 pubmed
    ..Thus, we have developed a versatile model for monitoring NF-kappaB activation in vivo. ..
  68. Branchini B, Southworth T, Murtiashaw M, Boije H, Fleet S. A mutagenesis study of the putative luciferin binding site residues of firefly luciferase. Biochemistry. 2003;42:10429-36 pubmed
    Firefly luciferase catalyzes the highly efficient emission of yellow-green light from substrate firefly luciferin by a sequence of reactions that require Mg-ATP and molecular oxygen. We had previously developed [Branchini, B. R...
  69. Kim Choi E, Danilo C, Kelly J, Carroll R, Shonnard D, Rybina I. Kinetic characterization and in vitro toxicity evaluation of a luciferase less susceptible to HPV chemical inhibition. Toxicol In Vitro. 2006;20:1537-47 pubmed
    ..The performance of CNBLuc03-06 in cell-based in vitro toxicity assays was shown to be superior to the current commercial formulation. ..
  70. Vlasova T, Ugarova N. Quenching of the fluorescence of Tyr and Trp residues of firefly luciferase from Luciola mingrelica by the substrates. Biochemistry (Mosc). 2007;72:962-7 pubmed
    ..The observed effects are probably connected with conformational changes in the luciferase molecule upon its interaction with the substrates. ..
  71. Neunzig I, Dragan C, Widjaja M, Schwaninger A, Peters F, Maurer H, et al. Whole-cell biotransformation assay for investigation of the human drug metabolizing enzyme CYP3A7. Biochim Biophys Acta. 2011;1814:161-7 pubmed publisher
    ..We believe that these new tools for a fast and easy investigation of substrates and inhibitors of human CYP3A7 will contribute to the gain of important insights for drug metabolism, efficacy and safety. ..
  72. Zaitseva M, Kapnick S, Golding H. Measurements of vaccinia virus dissemination using whole body imaging: approaches for predicting of lethality in challenge models and testing of vaccines and antiviral treatments. Methods Mol Biol. 2012;890:161-76 pubmed publisher
  73. Crouch S, Kozlowski R, Slater K, Fletcher J. The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity. J Immunol Methods. 1993;160:81-8 pubmed
    ..It would therefore appear to be possible to use ATP bioluminescence in the detection of cytokine activity in a number of different bioassays. ..
  74. Sorensen C, Novak I. Visualization of ATP release in pancreatic acini in response to cholinergic stimulus. Use of fluorescent probes and confocal microscopy. J Biol Chem. 2001;276:32925-32 pubmed
    ..In a close vicinity of acini we detect about 9 microm ATP after cholinergic stimulation. Thus, ATP is poised as the paracrine mediator between pancreatic acini and ducts. ..
  75. Maeda M. [Determination of biological substances using bioluminescent reaction based on luciferin-luciferase]. Rinsho Byori. 2004;52:595-603 pubmed
    ..In this article, the use of various enzymes as labels in the design and development of immunoassays, and the combination of PCR/novel bioluminescent pyrophosphate assay, for detecting biomolecules is reviewed. ..
  76. Wender P, Goun E, Jones L, Pillow T, Rothbard J, Shinde R, et al. Real-time analysis of uptake and bioactivatable cleavage of luciferin-transporter conjugates in transgenic reporter mice. Proc Natl Acad Sci U S A. 2007;104:10340-5 pubmed
  77. Conley N, Dragulescu Andrasi A, Rao J, Moerner W. A selenium analogue of firefly D-luciferin with red-shifted bioluminescence emission. Angew Chem Int Ed Engl. 2012;51:3350-3 pubmed publisher
    ..It has a red-shifted bioluminescence emission maximum at 600 nm and is suitable for bioluminescence imaging studies in living subjects. ..
  78. Wilson T, Hastings J. Bioluminescence. Annu Rev Cell Dev Biol. 1998;14:197-230 pubmed
  79. Björkman K, Karl D. A novel method for the measurement of dissolved adenosine and guanosine triphosphate in aquatic habitats: applications to marine microbial ecology. J Microbiol Methods. 2001;47:159-67 pubmed
    ..formed Mg(OH)(2) and can be concentrated by factors greater than 200-fold, for subsequent measurement by the firefly luciferin-luciferase bioluminescence reaction...
  80. Graff R, Lee G. Microplate live cell assay system for early events in mechanotransduction. Anal Biochem. 2003;318:181-6 pubmed
    ..The live-cell assay using fluid injection as a mechanical stimulus is a valuable tool for dissecting the role of signaling pathways in mechanotransduction. ..
  81. Day J, Bailey M. Structure and evolution of the luciferin-regenerating enzyme (LRE) gene from the firefly Photinus pyralis. Insect Mol Biol. 2003;12:365-72 pubmed
    ..gambiae and mammalian SMP30 proteins but not Drosophila. LRE and luciferase sequence comparisons revealed two conserved putative luciferin-binding sites. The evolution of LRE was discussed in relation to its function. ..
  82. Day J, Tisi L, Bailey M. Evolution of beetle bioluminescence: the origin of beetle luciferin. Luminescence. 2004;19:8-20 pubmed
    ..This review examines recent novel insights into beetle luciferin recycling and we assess a range of possible biosynthetic mechanisms. ..
  83. Zhou W, Valley M, Shultz J, Hawkins E, Bernad L, Good T, et al. New bioluminogenic substrates for monoamine oxidase assays. J Am Chem Soc. 2006;128:3122-3 pubmed
    ..This design strategy should be applicable to fluorogenic MAO substrates and could broaden the structural requirements of substrates for other enzyme assays. ..
  84. Viviani V, Bechara E, Ohmiya Y. Cloning, sequence analysis, and expression of active Phrixothrix railroad-worms luciferases: relationship between bioluminescence spectra and primary structures. Biochemistry. 1999;38:8271-9 pubmed
  85. Berovic N, Pratontep S, Bryant A, Montouris A, Green R. The kinetics of radiation damage to the protein luciferase and recovery of enzyme activity after irradiation. Radiat Res. 2002;157:122-7 pubmed
    ..A few minutes after the pulse of protons, most of the enzyme activity has recovered. We attribute the fast recovery to the removal of charged ions, while the slow recovery involves refolding of denatured protein. ..
  86. Romøren K, Thu B, Evensen Ø. Immersion delivery of plasmid DNA. II. A study of the potentials of a chitosan based delivery system in rainbow trout (Oncorhynchus mykiss) fry. J Control Release. 2002;85:215-25 pubmed
    ..Careful selection of chitosan type and charge of the particles may result in a potential for chitosan based immersion delivery of plasmid DNA. ..
  87. Leskinen P, Virta M, Karp M. One-step measurement of firefly luciferase activity in yeast. Yeast. 2003;20:1109-13 pubmed
    ..0. The ease of use of the non-peroxisomal luciferase makes it an interesting alternative for reporter genes that are conventionally used in yeast, such as lacZ. ..
  88. Zhou W, Andrews C, Liu J, Shultz J, Valley M, Cali J, et al. Self-cleavable bioluminogenic luciferin phosphates as alkaline phosphatase reporters. Chembiochem. 2008;9:714-8 pubmed publisher
  89. Chung L, Hayashi S, Lundberg M, Nakatsu T, Kato H, Morokuma K. Mechanism of efficient firefly bioluminescence via adiabatic transition state and seam of sloped conical intersection. J Am Chem Soc. 2008;130:12880-1 pubmed publisher
  90. Buckley S, Howe S, Rahim A, Buning H, McIntosh J, Wong S, et al. Luciferin detection after intranasal vector delivery is improved by intranasal rather than intraperitoneal luciferin administration. Hum Gene Ther. 2008;19:1050-6 pubmed publisher
    ..Compared with intraperitoneal injection, intranasal instillation yields about a 10-fold increase in sensitivity with an approximate 30-fold reduction in luciferin usage when bioimaging in the nasal and pulmonary airways of mice. ..
  91. Presiado I, Erez Y, Simkovitch R, Shomer S, Gepshtein R, Pinto da Silva L, et al. Excited-state proton transfer of firefly dehydroluciferin. J Phys Chem A. 2012;116:10770-9 pubmed publisher
    ..In weakly acidic aqueous solutions, the deprotonated form's emission is efficiently quenched. We attribute this observation to the ground-state protonation of the thiazole nitrogen, whose pK(a) value is ~3. ..
  92. Katsuragi T, Tokunaga T, Ogawa S, Soejima O, Sato C, Furukawa T. Existence of ATP-evoked ATP release system in smooth muscles. J Pharmacol Exp Ther. 1991;259:513-8 pubmed