guanylyl imidodiphosphate


Summary: A non-hydrolyzable analog of GTP, in which the oxygen atom bridging the beta to the gamma phosphate is replaced by a nitrogen atom. It binds tightly to G-protein in the presence of Mg2+. The nucleotide is a potent stimulator of ADENYLYL CYCLASES.

Top Publications

  1. Springer S, Schekman R. Nucleation of COPII vesicular coat complex by endoplasmic reticulum to Golgi vesicle SNAREs. Science. 1998;281:698-700 pubmed
    ..The data suggest that transmembrane proteins can be taken up into COPII vesicles by direct interactions with the coat proteins and may play a structural role in the assembly of the COPII coat complex...
  2. Padmanabhan S, Freymann D. The conformation of bound GMPPNP suggests a mechanism for gating the active site of the SRP GTPase. Structure. 2001;9:859-67 pubmed
    ..The structural basis for the GTP-dependent interaction between the two proteins, however, remains unknown...
  3. Ma F, Ohkuma S, Kishi M, Kuriyama K. Ontogeny of beta-adrenergic receptor-mediated cyclic AMP generating system in primary cultured neurons. Int J Dev Neurosci. 1991;9:347-56 pubmed
  4. Ozawa H, Rasenick M. Chronic electroconvulsive treatment augments coupling of the GTP-binding protein Gs to the catalytic moiety of adenylyl cyclase in a manner similar to that seen with chronic antidepressant drugs. J Neurochem. 1991;56:330-8 pubmed
    ..These results suggest that chronic administration of ECS enhances coupling between Gs and adenylyl cyclase enzyme and modifies interactions between Gs and Gi. ..
  5. Roth B, McLean S, Zhu X, Chuang D. Characterization of two [3H]ketanserin recognition sites in rat striatum. J Neurochem. 1987;49:1833-8 pubmed
    ..Thus, in the striatum and septum, [3H]ketanserin labels a unique recognition site. This site has recently been shown to be associated with dopaminergic nerve endings and may regulate biogenic amine release. ..
  6. Minuzzi L, Cumming P. Agonist binding fraction of dopamine D2/3 receptors in rat brain: a quantitative autoradiographic study. Neurochem Int. 2010;56:747-52 pubmed publisher
    ..Thus [(3)H]NPA and [(3)H]raclopride binding sites have nearly identical abundances in rat dorsal striatum, but are distinct in the ventral striatum, and with respect to their displacement by LSD. ..
  7. Pai E, Kabsch W, Krengel U, Holmes K, John J, Wittinghofer A. Structure of the guanine-nucleotide-binding domain of the Ha-ras oncogene product p21 in the triphosphate conformation. Nature. 1989;341:209-14 pubmed
    ..Many interactions between nucleotide and protein have been identified. The effects of point mutations and the conservation of amino-acid sequence in the guanine-nucleotide-binding proteins are discussed. ..
  8. Sim Selley L, Xiao R, Childers S. Anatomical distribution of sodium-dependent [(3)H]naloxone binding sites in rat brain. Synapse. 2000;35:256-64 pubmed
    ..These studies revealed the presence of a subpopulation of [(3)H]naloxone binding sites which are sodium-dependent and have a unique regional distribution in the rat brain. ..
  9. Lardong J, Driller J, Depner H, Weise C, Petzoldt A, Wahl M, et al. Structures of Drosophila melanogaster Rab2 and Rab3 bound to GMPPNP. Acta Crystallogr F Struct Biol Commun. 2015;71:34-40 pubmed publisher
    ..Here, high-resolution crystal structures of Rab2 and Rab3 in complex with GMPPNP and Mg2+ are presented. In the structure of Rab3 a modified cysteine residue is observed with an enigmatic electron density attached to its thiol function. ..

More Information

Publications114 found, 100 shown here

  1. Gentile D, Rathinaswamy M, Jenkins M, Moss S, Siempelkamp B, Renslo A, et al. Ras Binder Induces a Modified Switch-II Pocket in GTP and GDP States. Cell Chem Biol. 2017;24:1455-1466.e14 pubmed publisher
    ..Targeting both nucleotide states opens the possibility of inhibiting oncogenic mutants of Ras, which exist predominantly in the GTP state in cells. ..
  2. Freissmuth M, Selzer E, Schutz W. Interactions of purified bovine brain A1-adenosine receptors with G-proteins. Reciprocal modulation of agonist and antagonist binding. Biochem J. 1991;275 ( Pt 3):651-6 pubmed
  3. Muma N, Mariyappa R, Williams K, Lee J. Differences in regional and subcellular localization of G(q/11) and RGS4 protein levels in Alzheimer's disease: correlation with muscarinic M1 receptor binding parameters. Synapse. 2003;47:58-65 pubmed
  4. Holohean A, Hackman J. Mechanisms intrinsic to 5-HT2B receptor-induced potentiation of NMDA receptor responses in frog motoneurones. Br J Pharmacol. 2004;143:351-60 pubmed
  5. Abrahams J, Kraal B, Clark B, Bosch L. Isolation and stability of ternary complexes of elongation factor Tu, GTP and aminoacyl-tRNA. Nucleic Acids Res. 1991;19:553-7 pubmed
    ..In the latter procedure, the solubility characteristics of the ternary complexes are highly reminiscent to those of free tRNA. Concentrated samples of EF-Tu.GMPPNP.aminoacyl-tRNA complexes show a high stability. ..
  6. Morgan A, Burgoyne R. Stimulation of Ca2(+)-independent catecholamine secretion from digitonin-permeabilized bovine adrenal chromaffin cells by guanine nucleotide analogues. Relationship to arachidonate release. Biochem J. 1990;269:521-6 pubmed
  7. Makimura M, Murakoshi Y. Kappa-opioid agonists do not inhibit adenylate cyclase. J Pharmacobiodyn. 1989;12:125-31 pubmed
    ..These results suggest that the signalling transduction of mu- and delta-agonists is partially mediated by the adenylate cyclase system coupled with opioid receptors, but that kappa-receptors may not be associated with adenylate cyclase. ..
  8. Nogueira C, Rotta L, Perry M, Souza D, da Rocha J. Diphenyl diselenide and diphenyl ditelluride affect the rat glutamatergic system in vitro and in vivo. Brain Res. 2001;906:157-63 pubmed
    ..These results suggest that the toxic effects of organochalcogens could result from action at different levels of neural signal transduction pathways, possibly involving other neurotransmitters besides the glutamatergic system. ..
  9. Stohr J, Novotny J, Svoboda P. Characterization of [3H]-forskolin binding sites in young and adult rat brain cortex: identification of suramin as a competitive inhibitor of [3H]-forskolin binding. Can J Physiol Pharmacol. 2005;83:573-81 pubmed
    ..Results of our experiments with suramin indicated that this drug may act as a competitive inhibitor of [3H]-forskolin binding. ..
  10. Trahey M, McCormick F. A cytoplasmic protein stimulates normal N-ras p21 GTPase, but does not affect oncogenic mutants. Science. 1987;238:542-5 pubmed
    ..Furthermore, it appears that the major effect of position 12 mutations is to prevent this protein from stimulating p21 GTPase activity, thereby allowing these mutants to remain in the active GTP-bound state. ..
  11. Loucif A, Bonnavion P, Macri B, Golmard J, Boni C, Melfort M, et al. Gender-dependent regulation of G-protein-gated inwardly rectifying potassium current in dorsal raphe neurons in knock-out mice devoid of the 5-hydroxytryptamine transporter. J Neurobiol. 2006;66:1475-88 pubmed
    ..Accordingly, gender differences in central 5-HT neurotransmission appear to depend-at least in part-on sex-related variations in corresponding receptor-G protein signaling mechanisms. ..
  12. Hori T, Yamamoto T, Hatta K, Moroji T. Modulation of Mg(2+)-dependent [3H]TCP binding by L-glutamate, glycine, and guanine nucleotides in rat cerebral cortex. Synapse. 1991;8:13-21 pubmed
    ..abstract truncated at 250 words) ..
  13. Tang C, Yang J, Wu L, Dong L, Liu M. Phosphorylation of beta-adrenergic receptor leads to its redistribution in rat heart during sepsis. Am J Physiol. 1998;274:R1078-86 pubmed
  14. Tang S, He W, Xu H, Liu W, Ruan K. Eukaryotic elongation factor 2 can bind to the synthetic oligoribonucleotide that mimics sarcin/ricin domain of rat 28S ribosomal RNA. Mol Cell Biochem. 2001;223:117-21 pubmed
    ..These results indicate that eEF2 is likely to bind directly to the sarcin/ricin domain of 28S ribosomal RNA in the process of protein synthesis. ..
  15. Gronwald W, Huber F, Grunewald P, Sporner M, Wohlgemuth S, Herrmann C, et al. Solution structure of the Ras binding domain of the protein kinase Byr2 from Schizosaccharomyces pombe. Structure. 2001;9:1029-41 pubmed
    ..However, details of the 3D structure and the interacting interface are different, thereby determining the specifity of the recognition of Ras and Ras-related proteins. ..
  16. Coupry I, Duzic E, Lanier S. Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. II. Preferential coupling of the alpha 2C-adrenergic receptor to the guanine nucleotide-binding protein, Go. J Biol Chem. 1992;267:9852-7 pubmed
    ..This report indicates the utility of R-G cotransfection in mammalian cells as a "natural environment model" to characterize events occurring at the R-G and G-E interface. ..
  17. Georgoussi Z, Merkouris M, Mullaney I, Megaritis G, Carr C, Zioudrou C, et al. Selective interactions of mu-opioid receptors with pertussis toxin-sensitive G proteins: involvement of the third intracellular loop and the c-terminal tail in coupling. Biochim Biophys Acta. 1997;1359:263-74 pubmed
    ..Peptide i3.1 also inhibited DAMGO-stimulated [35S]guanosine-5'-O-(3-thio)triphosphate ([35S]GTP-gammaS) binding in the same membranes. In contrast, peptides derived from the second intracellular loop were without any effect. ..
  18. Avissar S, Nechamkin Y, Roitman G, Schreiber G. Dynamics of ECT normalization of low G protein function and immunoreactivity in mononuclear leukocytes of patients with major depression. Am J Psychiatry. 1998;155:666-71 pubmed
    ..G protein measurements in patients with depression may potentially serve not only as a biochemical marker for affective state but also for biochemical prediction and evaluation of responses to ECT. ..
  19. Ohm T, von Dewitz G, Witte K, Nitsch R, Lemmer B. Basal and stimulated hippocampal adenylate cyclase activity in the experimentally lesioned rat entorhinal cortex. Acta Neuropathol. 1999;98:389-95 pubmed
    ..Our data suggests that the loss of entorhinal afferents alone cannot explain the reduction in cyclase-activity seen in 'entorhinal' cases. ..
  20. Hartmann J, Scepek S, Hafez I, Lindau M. Differential regulation of exocytotic fusion and granule-granule fusion in eosinophils by Ca2+ and GTP analogs. J Biol Chem. 2003;278:44929-34 pubmed
    ..Granule fusions with the three different target compartments thus involve different regulatory mechanisms. ..
  21. Yamashita A, Kurokawa T, Dan ura T, Yanagiuchi H, Ishibashi S. Protein kinases induce isoproterenol desensitization of beta-adrenoceptor-coupled adenylate cyclase system: significance of receptor occupancy. Eur J Pharmacol. 1987;143:19-26 pubmed
    ..These results suggest that cyclic AMP- or phorbol ester-induced desensitization is enhanced by receptor occupation by beta-adrenoceptor agonists...
  22. Dias S, Cerione R. X-ray crystal structures reveal two activated states for RhoC. Biochemistry. 2007;46:6547-58 pubmed
    ..These and other differences regarding the changes in the switch domains accompanying the activation of RhoA and RhoC provide plausible explanations for the functional specificity exhibited by the two GTPases. ..
  23. Fenwick R, Prasannan S, Campbell L, Nietlispach D, Evetts K, Camonis J, et al. Solution structure and dynamics of the small GTPase RalB in its active conformation: significance for effector protein binding. Biochemistry. 2009;48:2192-206 pubmed publisher
    ..In contrast, 31P NMR analysis of RalB.GTP reveals that conformations corresponding to states 1 and 2 are both sampled in solution and that addition of an effector protein only partially stabilizes state 2. ..
  24. Hawkins M, O Connor S, Radulovacki M, Bowersox S, Mignot E, Dement W. Radioligand binding to adenosine receptors and adenosine uptake sites in different brain regions of normal and narcoleptic dogs. Pharmacol Biochem Behav. 1991;38:1-6 3 pubmed
    ..A characterization of these low- and high-affinity sites was performed and results indicated that these sites cannot be explained by a single interaction of the A1 receptor with a single G-protein population. ..
  25. De Vendittis E, Fasano O. Energetic aspects of intramolecular coupling between the nucleotide binding site and the distal switch II region of the yeast RAS2 protein. FEBS Lett. 1994;347:133-6 pubmed
  26. Tang C, Liu M. Initial externalization followed by internalization of beta-adrenergic receptors in rat heart during sepsis. Am J Physiol. 1996;270:R254-63 pubmed
  27. Junutula J, Schonteich E, Wilson G, Peden A, Scheller R, Prekeris R. Molecular characterization of Rab11 interactions with members of the family of Rab11-interacting proteins. J Biol Chem. 2004;279:33430-7 pubmed
    ..The entropy and enthalpy analyses suggest that binding to Rab11a/b may induce conformational changes in FIPs. ..
  28. Hirasawa N, Hoshi T, Kudoh M, Mue S, Tsurufuji S, Watanabe M, et al. Analysis of the leukotriene D4 receptor in the granulation tissue of allergic inflammation in rats. Int Arch Allergy Immunol. 1992;99:107-11 pubmed
    ..These results indicate that LTD4 binding sites in the granulation tissue are high affinity receptors for LTD4. A possible role of LTD4 in the recurrence of allergic inflammation in the chronic phase is discussed. ..
  29. Avissar S, Schreiber G. Measurement of early events in signal transduction beyond receptors involving G proteins function in mononuclear leucocytes. J Neuroimmunol. 1996;70:81-6 pubmed
  30. Maeda Y, Kuroki R, Haase W, Michel H, Reiländer H. Comparative analysis of high-affinity ligand binding and G protein coupling of the human CXCR1 chemokine receptor and of a CXCR1-Galpha fusion protein after heterologous production in baculovirus-infected insect cells. Eur J Biochem. 2004;271:1677-89 pubmed
    ..The addition of guanyl-5'-yl imidodiphosphate resulted in a dramatic reduction of the number of binding sites; however, the K(i) values remained unchanged, indicating coupling of the receptor to the guanine nucleotide-binding protein. ..
  31. Iczkowski K, Omara Opyene A, Kulkarni T, Pansara M, Shah G. Paracrine calcitonin in prostate cancer is linked to CD44 variant expression and invasion. Anticancer Res. 2005;25:2075-83 pubmed
    ..This paracrine factor, acting though cyclic AMP, regulates the expression of CD44v7-10, which modulates the tumor phenotype. ..
  32. Pyne N, Grady M, Shehnaz D, Stevens P, Pyne S, Rodger I. Muscarinic blockade of beta-adrenoceptor-stimulated adenylyl cyclase: the role of stimulatory and inhibitory guanine-nucleotide binding regulatory proteins (Gs and Gi). Br J Pharmacol. 1992;107:881-7 pubmed
    ..These results together may explain the functional antagonism that exists between increased muscarinic tone and the ability of beta-adrenoceptor agonists to provoke excitation-contraction uncoupling. ..
  33. Sorota S, Chlenov M, Du X, Kagan M. ATP-dependent activation of the atrial acetylcholine-induced K+ channel does not require nucleoside diphosphate kinase activity. Circ Res. 1998;82:971-9 pubmed
    ..We hypothesize that the activation by millimolar concentrations of ATP is due to binding of adenine nucleotide to the guanine nucleotide binding site of the G protein(s) responsible for stimulating the acetylcholine-induced K+ current. ..
  34. Gurdal H, Friedman E, Johnson M. Beta-adrenoceptor-G alpha S coupling decreases with age in rat aorta. Mol Pharmacol. 1995;47:772-8 pubmed
    ..abstract truncated at 400 words) ..
  35. Marcellino D, Carriba P, Filip M, Borgkvist A, Frankowska M, Bellido I, et al. Antagonistic cannabinoid CB1/dopamine D2 receptor interactions in striatal CB1/D2 heteromers. A combined neurochemical and behavioral analysis. Neuropharmacology. 2008;54:815-23 pubmed publisher
    ..Taken together, these results give evidence for the existence of antagonistic CB(1)/D(2) receptor-receptor interactions within CB(1)/D(2) heteromers in which A(2A) receptors may also participate. ..
  36. Fan T, Liang C, Kawashima S, Banerjee S. Alterations in cardiac beta-adrenoceptor responsiveness and adenylate cyclase system by congestive heart failure in dogs. Eur J Pharmacol. 1987;140:123-32 pubmed
    ..This decreased activation of adenylate cyclase by beta-agonists may be responsible, at least in part, for the diminished cardiac inotropic response to catecholamines in congestive heart failure. ..
  37. Esters H, Alexandrov K, Constantinescu A, Goody R, Scheidig A. High-resolution crystal structure of S. cerevisiae Ypt51(DeltaC15)-GppNHp, a small GTP-binding protein involved in regulation of endocytosis. J Mol Biol. 2000;298:111-21 pubmed
    ..Sequence comparisons between the different isoforms Ypt51, Ypt52 and Ypt53 provide the first insights into determinants for specific effector binding and for fine-tuning of the intrinsic GTP-hydrolysis rate. ..
  38. Clemens S, Katz P. Identified serotonergic neurons in the Tritonia swim CPG activate both ionotropic and metabotropic receptors. J Neurophysiol. 2001;85:476-9 pubmed
    ..A similar synaptic activation of metabotropic receptors might also be found within the CPG itself, where it could exert a direct influence onto motor pattern generation...
  39. Gurguis G, Turkka J, Linnoila M. Effects of serotonin and metergoline on 125[I]-iodocyanopindolol binding parameters to beta-adrenergic receptors in rat brain. Eur Neuropsychopharmacol. 1998;8:131-40 pubmed
    ..Neither serotonin nor metergoline had an effect on the agonist isoproterenol dissociation constant from betaAR in either conformational state. ..
  40. Dzeja P, Bortolon R, Perez Terzic C, Holmuhamedov E, Terzic A. Energetic communication between mitochondria and nucleus directed by catalyzed phosphotransfer. Proc Natl Acad Sci U S A. 2002;99:10156-61 pubmed
    ..Thus, mitochondrial oxidative phosphorylation coupled with phosphotransfer relays provides an efficient energetic unit in support of nuclear transport. ..
  41. Slater S, Cook A, Seiz J, Malinowski S, Stagliano B, Stubbs C. Effects of ethanol on protein kinase C alpha activity induced by association with Rho GTPases. Biochemistry. 2003;42:12105-14 pubmed
    ..The results also suggest that the effects of ethanol on Rho GTPase-induced activity may switch between an activation and inhibition depending on the concentration of Ca(2+) and other activators. ..
  42. Verrotti A, Crechet J, Di Blasi F, Seidita G, Mirisola M, Kavounis C, et al. RAS residues that are distant from the GDP binding site play a critical role in dissociation factor-stimulated release of GDP. EMBO J. 1992;11:2855-62 pubmed
    ..These findings point to residues 80-82 as important determinants of the response of RAS to GDP dissociation factors. This suggests a molecular model for the enhancement of nucleotide release from RAS by such factors. ..
  43. Ribas C, Sato M, Hildebrandt J, Lanier S. Analysis of signal transfer from receptor to Go/Gi in different membrane environments and receptor-independent activators of brain G protein. Methods Enzymol. 2002;344:140-52 pubmed
  44. Tong J, Ross B, Schmunk G, Peretti F, Kalasinsky K, Furukawa Y, et al. Decreased striatal dopamine D1 receptor-stimulated adenylyl cyclase activity in human methamphetamine users. Am J Psychiatry. 2003;160:896-903 pubmed
    ..Decreased dopamine D(1) receptor function might underlie part of the known (drug withdrawal syndrome) or expected (drug tolerance) consequences of methamphetamine exposure in humans. ..
  45. Sastre M, García Sevilla J. Alpha 2-adrenoceptor subtypes identified by [3H]RX821002 binding in the human brain: the agonist guanoxabenz does not discriminate different forms of the predominant alpha 2A subtype. J Neurochem. 1994;63:1077-85 pubmed
    ..The results indicate that in the human brain the predominant alpha 2-adrenoceptor is of the alpha 2A subtype and that this functionally relevant receptor subtypes is not heterogeneous in nature. ..
  46. Coquil J, Brunelle G. Adenylate cyclase of platelets from spontaneously hypertensive rats: reduction of the inhibition by GTP. Biochem Biophys Res Commun. 1989;162:1265-71 pubmed
    ..1 microM prostaglandin E1. These results suggest that the modulatory effect of the guanine nucleotide inhibitory protein on adenylate cyclase may be reduced in platelets from SHR. ..
  47. Brodde O, Zerkowski H, Doetsch N, Khamssi M. Subtype-selective up-regulation of human saphenous vein beta 2-adrenoceptors by chronic beta-adrenoceptor antagonist treatment. Naunyn Schmiedebergs Arch Pharmacol. 1989;339:479-82 pubmed
    ..It is concluded that beta-adrenoceptor antagonists without ISA increase human saphenous vein beta 2-adrenoceptors in a subtype-selective fashion...
  48. Sarda N, Gharib A, Reynaud D, Ou L, Pacheco H. Identification of adenosine receptor in rat pineal gland: evidence for A-2 selectivity. J Neurochem. 1989;53:733-7 pubmed
    ..Binding was also displaced by 3-isobutyl-1-methylxanthine (IC50 = 23.6 microM). These findings are consistent with the selective labeling by [3H]NECA of an A-2-type adenosine receptor in rat pineal membranes. ..
  49. Inoue M, Imanaga I. Phosphatase is responsible for run down, and probably G protein-mediated inhibition of inwardly rectifying K+ currents in guinea pig chromaffin cells. J Gen Physiol. 1995;105:249-66 pubmed
  50. Badiani K, Arthur G. Evidence for receptor and G-protein regulation of a phosphatidylethanolamine-hydrolysing phospholipase A1 in guinea-pig heart microsomes: stimulation of phospholipase A1 activity by DL-isoprenaline and guanine nucleotides. Biochem J. 1995;312 ( Pt 3):805-9 pubmed
    ..Furthermore the stimulation of phospholipase A1 activity by the agonist may be mediated via activation of G-proteins...
  51. Plourde G, Martin M, Rousseau Migneron S, Nadeau A. Effect of physical training on ventricular beta-adrenergic receptor adenylate cyclase system of diabetic rats. Metabolism. 1991;40:362-7 pubmed
    ..These data suggest that the beneficial effects observed in response to training in experimental diabetes are not associated with changes in beta-adrenergic receptor adenylate cyclase system on membranes from ventricular tissue. ..
  52. Aronstam R, Carrier G. Insulin prevention of altered muscarinic receptor-G protein coupling in diabetic rat atria. Diabetes. 1989;38:1611-6 pubmed
  53. Zhang S, Yue Y, Xing X, Wei T, Yao W, Han J. Adenylate cyclase-modulating mediators of insulin. Sci Sin B. 1988;31:838-49 pubmed
    ..The results that both mediators combined with Gpp(NH)p and forskolin to affect the enzyme activity show that the action of insulin mediators likely resides in the GTP regulatory component of adenylate cyclase. ..
  54. Nagakura A, Miyake Takagi K, Takagi N, Fukui M, Takeo S. Impairment of adenylyl cyclase and of spatial memory function after microsphere embolism in rats. J Neurosci Res. 2002;68:363-72 pubmed
    ..CaM)-sensitive AC in the ipsilateral hemisphere throughout the experiment, whereas the basal and 5'-guanylyl imidodiphosphate (Gpp(NH)p)-sensitive AC activities were not altered...
  55. Vasilyeva O, Kolygo K, Leonova Y, Potapenko N, Ovchinnikova T. Domain structure and ATP-induced conformational changes in Escherichia coli protease Lon revealed by limited proteolysis and autolysis. FEBS Lett. 2002;526:66-70 pubmed
    ..Obvious resistance of the protease Lon C-terminus to proteolysis indicates that this region of the enzyme molecule including its substrate-binding and proteolytic domains has a well folded structure. ..
  56. Brunk I, Blex C, Rachakonda S, Höltje M, Winter S, Pahner I, et al. The first luminal domain of vesicular monoamine transporters mediates G-protein-dependent regulation of transmitter uptake. J Biol Chem. 2006;281:33373-85 pubmed
    ..The first luminal loop of VMATs may represent a G-protein-coupled receptor that adapts vesicular filling. ..
  57. Hagiwara M, Kobayashi K, Tadokoro T, Yamamoto Y. Rab5 affinity chromatography without nonhydrolyzable GTP analogues. Z Naturforsch C. 2009;64:303-6 pubmed
    ..To validate this method, the binding of EEA-1 was confirmed and several novel Rab5-binding proteins were also identified by 2-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). ..
  58. Liebmann C, Graness A, Boehmer A, Kovalenko M, Adomeit A, Steinmetzer T, et al. Tyrosine phosphorylation of GSalpha and inhibition of bradykinin-induced activation of the cyclic AMP pathway in A431 cells by epidermal growth factor receptor. J Biol Chem. 1996;271:31098-105 pubmed
    ..We propose that Gsalpha may represent a key regulatory protein in the cross-talk between the signal transduction pathways of BK and EGF in A431 cells. ..
  59. Mader S, Alley P. Age-related changes in adenylyl cyclase activity in rat aorta membranes. Mech Ageing Dev. 1998;101:111-8 pubmed
    ..These data suggest that using a membrane system to assess age-related changes in beta-adrenergic responsiveness in vascular smooth muscle does not retain the robust differences seen in whole vessels. ..
  60. Rotta L, Nogueira C, da Silva L, Soares F, Perry M, Souza D. Effect of undernutrition on GMP-PNP binding and adenylate cyclase activity from rat brain. Cell Mol Neurobiol. 2002;22:365-72 pubmed
    ..This effect was less pronounced or even absent in old rats. 3. Ontogenetically, the dietary treatment effect might be interpreted as a retarded development associated with protein malnutrition. ..
  61. Iuga A, Spoerner M, Kalbitzer H, Brunner E. Solid-state 31P NMR spectroscopy of microcrystals of the Ras protein and its effector loop mutants: comparison between crystalline and solution state. J Mol Biol. 2004;342:1033-40 pubmed
    ..The results obtained for the wild-type protein are compared with the behavior of GppNHp complexes of the effector loop mutants Ras(T35S) and Ras(T35A). These mutants prefer a conformation similar to the GDP bound "off" state. ..
  62. Bennett V, Perrine S, Simmons M. Neurokinin-1 receptor resensitization precedes receptor recycling. J Pharmacol Exp Ther. 2005;313:1347-54 pubmed
    ..These data show that the resensitization of responsiveness to substance P precedes receptor recycling. This may result from a conversion of nonfunctional neurokinin-1 receptors to functional receptors at the plasma membrane. ..
  63. Harris Warrick R, Hammond C, Paupardin Tritsch D, Homburger V, Rouot B, Bockaert J, et al. An alpha 40 subunit of a GTP-binding protein immunologically related to Go mediates a dopamine-induced decrease of Ca2+ current in snail neurons. Neuron. 1988;1:27-32 pubmed
    ..We propose that this is a 40 kd alpha subunit of a molluscan G protein immunologically related to alpha o and that it mediates the effect of dopamine on Ca2+ currents in identified snail neurons. ..
  64. Livingstone M. Genetic dissection of Drosophila adenylate cyclase. Proc Natl Acad Sci U S A. 1985;82:5992-6 pubmed
    ..The results suggest that the component of cyclase activity that is stimulated by calcium/calmodulin, possibly a genetically distinct catalytic subunit, is not coupled to the G subunit or the G subunit/monoamine receptor complex. ..
  65. Dittmann J, Keller C, Harisch G. Inhibition of adenylate cyclase of rat hepatic membranes by glycosaminoglycans. Life Sci. 1998;63:2199-208 pubmed
    ..Further characterization of enzyme inhibition mediated by dermatan sulfate shows that this molecule exerts an inhibitory effect of mixed type. ..
  66. Gawronski Salerno J, Freymann D. Structure of the GMPPNP-stabilized NG domain complex of the SRP GTPases Ffh and FtsY. J Struct Biol. 2007;158:122-8 pubmed
    ..It also reveals a K+ coordination site at the highly conserved DARGG loop at the N/G interdomain interface. ..
  67. Culp S, Rominger D, Tam S, De Souza E. [3H]DuP 734 [1-(cyclopropylmethyl)-4-(2'-(4''-fluorophenyl)-2'- oxoethyl)-piperidine HBr]: a receptor binding profile of a high-affinity novel sigma receptor ligand in guinea pig brain. J Pharmacol Exp Ther. 1992;263:1175-87 pubmed
    ..In summary, [3H]DuP 734 is a novel ligand that binds with high affinity to sigma receptors in brain. ..
  68. McClue S, Milligan G. The alpha 2B adrenergic receptor of undifferentiated neuroblastoma x glioma hybrid NG108-15 cells, interacts directly with the guanine nucleotide binding protein, Gi2. FEBS Lett. 1990;269:430-4 pubmed
    ..These results demonstrate a direct interaction of the alpha 2B adrenergic receptor of NG108-15 cells with Gi2. ..
  69. Niefind K, Pütter M, Guerra B, Issinger O, Schomburg D. GTP plus water mimic ATP in the active site of protein kinase CK2. Nat Struct Biol. 1999;6:1100-3 pubmed
    ..An understanding of the structural basis of dual-cosubstrate specificity may help in the design of drugs that target CK2 or other kinases with this property. ..
  70. Pineau N, Lelievre V, Goursaud S, Hilairet S, Waschek J, Janet T, et al. The polypeptide PHI discriminates a GTP-insensitive form of VIP receptor in liver membranes. Neuropeptides. 2001;35:117-26 pubmed
  71. Supek F, Madden D, Hamamoto S, Orci L, Schekman R. Sec16p potentiates the action of COPII proteins to bud transport vesicles. J Cell Biol. 2002;158:1029-38 pubmed
    ..We propose that Sec16p nucleates a Sar1-GTP-dependent initiation of COPII assembly and serves to stabilize the coat to premature disassembly after Sar1p hydrolyzes GTP...
  72. Hattori Y, Sakuma I, Nakao Y, Kanno M. Pharmacological analysis of the cardiac actions of xamoterol, a beta adrenoceptor antagonist with partial agonistic activity, in guinea pig heart: evidence for involvement of adenylate cyclase system in its cardiac stimulant actions. J Pharmacol Exp Ther. 1987;242:1077-85 pubmed
    ..abstract truncated at 250 words)..
  73. Olson M, Schimmer B. Receptor-G-protein coupling in desensitization-resistant mutant adrenal cells. Endocrinology. 1994;134:7-10 pubmed
    ..These results indicate that the DR mutant may resist desensitization by affecting agonist-induced uncoupling of receptors from their guanyl nucleotide-binding regulatory G-proteins. ..
  74. Assie M, Cosi C, Koek W. Correlation between low/high affinity ratios for 5-HT(1A) receptors and intrinsic activity. Eur J Pharmacol. 1999;386:97-103 pubmed
    ..Their effects, however, appear to be G(i) protein-dependent. ..
  75. Zhang S, Haldenwang W. Guanine nucleotides stabilize the binding of Bacillus subtilis Obg to ribosomes. Biochem Biophys Res Commun. 2004;322:565-9 pubmed
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