guanosine diphosphate


Summary: A guanine nucleotide containing two phosphate groups esterified to the sugar moiety.

Top Publications

  1. Abankwa D, Hanzal Bayer M, Ariotti N, Plowman S, Gorfe A, Parton R, et al. A novel switch region regulates H-ras membrane orientation and signal output. EMBO J. 2008;27:727-35 pubmed publisher
    ..The results illustrate how the plasma membrane spatially constrains signalling conformations by acting as a semi-neutral interaction partner. ..
  2. Kimura T, Taniguchi S, Niki I. Actin assembly controlled by GDP-Rab27a is essential for endocytosis of the insulin secretory membrane. Arch Biochem Biophys. 2010;496:33-7 pubmed publisher
    ..These results suggest that coronin 3 is a genuine GDP-Rab27a effector, and that controls endocytosis of the secretory membrane via modulating actin assembly in pancreatic beta-cells. ..
  3. Bulley S, Wright M, Rommens C, Yan H, Rassam M, Lin Wang K, et al. Enhancing ascorbate in fruits and tubers through over-expression of the L-galactose pathway gene GDP-L-galactose phosphorylase. Plant Biotechnol J. 2012;10:390-7 pubmed publisher
    ..In both strawberry and tomato, an increase in polyphenolic content was associated with increased ascorbate. These results show that GGP can be used to raise significantly ascorbate concentration in commercially significant edible crops. ..
  4. Chung K, Rasmussen S, Liu T, Li S, DeVree B, Chae P, et al. Conformational changes in the G protein Gs induced by the ?2 adrenergic receptor. Nature. 2011;477:611-5 pubmed publisher
    ..As with the Ras family of small-molecular-weight G proteins, P-loop stabilization and ?-phosphate coordination are key determinants of GDP (and GTP) binding affinity...
  5. Jennings M, Pavitt G. eIF5 has GDI activity necessary for translational control by eIF2 phosphorylation. Nature. 2010;465:378-81 pubmed publisher
    ..Together our studies define a new step in the translation initiation pathway, one that is critical for normal translational controls. ..
  6. Gasper R, Meyer S, Gotthardt K, Sirajuddin M, Wittinghofer A. It takes two to tango: regulation of G proteins by dimerization. Nat Rev Mol Cell Biol. 2009;10:423-9 pubmed publisher
    ..We propose that the juxtaposition of the G domains of two monomers across the GTP-binding sites activates the biological function of these proteins and the GTPase reaction...
  7. K ster S, Wehner M, Herrmann C, K hlbrandt W, Yildiz O. Structure and function of the FeoB G-domain from Methanococcus jannaschii. J Mol Biol. 2009;392:405-19 pubmed publisher
    ..Together with the C-terminal helix, this loop may transmit the information about the nucleotide-bound state from the G-domain to the transmembrane region of FeoB...
  8. Figueiredo A, Wasmeier C, Tarafder A, Ramalho J, Baron R, Seabra M. Rab3GEP is the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes. J Biol Chem. 2008;283:23209-16 pubmed publisher
    ..Our results indicate promiscuity in Rab GEF action and suggest that members of related but functionally distinct Rab subfamilies can be controlled by common activators. ..
  9. Louet M, Perahia D, Martinez J, Floquet N. A concerted mechanism for opening the GDP binding pocket and release of the nucleotide in hetero-trimeric G-proteins. J Mol Biol. 2011;411:298-312 pubmed publisher
    ..This model is discussed in the context of the G-protein-coupled receptor/G-protein interaction close to the cell membrane. ..

More Information


  1. Gorfe A, Grant B, McCammon J. Mapping the nucleotide and isoform-dependent structural and dynamical features of Ras proteins. Structure. 2008;16:885-96 pubmed publisher
    ..We identified a number of isoform-specific, long-range side chain interactions that define unique pathways of communication between the nucleotide binding site and the C terminus. ..
  2. Aleksandrov A, Simonson T. Binding of tetracyclines to elongation factor Tu, the Tet repressor, and the ribosome: a molecular dynamics simulation study. Biochemistry. 2008;47:13594-603 pubmed publisher
    ..Overall, our results provide insights into the binding properties of tetracyclines and support the idea that EF-Tu is not their primary target. ..
  3. Wittinghofer A, Vetter I. Structure-function relationships of the G domain, a canonical switch motif. Annu Rev Biochem. 2011;80:943-71 pubmed publisher
    ..Although the G domain uses a universally conserved switching mechanism, its structure, function, and GTPase reaction are modified for many different pathways and processes. ..
  4. Hauryliuk V, Mitkevich V, Eliseeva N, Petrushanko I, Ehrenberg M, Makarov A. The pretranslocation ribosome is targeted by GTP-bound EF-G in partially activated form. Proc Natl Acad Sci U S A. 2008;105:15678-83 pubmed publisher
    ..We propose that the active overall conformation of EF-G is attained only in complex with the ribosome in its "ratcheted state," with hybrid tRNA binding sites. ..
  5. Eng E, Jalilian A, Spasov K, Unger V. Characterization of a novel prokaryotic GDP dissociation inhibitor domain from the G protein coupled membrane protein FeoB. J Mol Biol. 2008;375:1086-97 pubmed
    ..Our findings suggest that Fe(II) uptake in bacteria involves a G protein regulatory pathway reminiscent of signaling mechanisms found in higher-order organisms. ..
  6. Shin Y, Yoon J, Jang T, Kim S, Heo W, So I, et al. Crystal structure of Rab6A'(Q72L) mutant reveals unexpected GDP/Mg²? binding with opened GTP-binding domain. Biochem Biophys Res Commun. 2012;424:269-73 pubmed publisher
    ..Large conformational changes were also detected in the switch I and switch II regions. Our structure revealed that the non-hydrolysable, constitutively active form of Rab6A' can accommodate GDP/Mg(2+) in the open conformation. ..
  7. Pucadyil T, Schmid S. Conserved functions of membrane active GTPases in coated vesicle formation. Science. 2009;325:1217-20 pubmed publisher
  8. Louet M, Martinez J, Floquet N. GDP release preferentially occurs on the phosphate side in heterotrimeric G-proteins. PLoS Comput Biol. 2012;8:e1002595 pubmed publisher
  9. Thomas C, Tall G, Adhikari A, Sprang S. Ric-8A catalyzes guanine nucleotide exchange on G alphai1 bound to the GPR/GoLoco exchange inhibitor AGS3. J Biol Chem. 2008;283:23150-60 pubmed publisher
    ..Galpha(i1) complex, even when present at very high concentrations. The action of Ric-8A on AGS3:Galpha(i1).GDP ensures unidirectional activation of Galpha subunits that cannot be reversed by AGS3. ..
  10. Huang B, Wu H, Hao N, Blombach F, van der Oost J, Li X, et al. Functional study on GTP hydrolysis by the GTP-binding protein from Sulfolobus solfataricus, a member of the HflX family. J Biochem. 2010;148:103-13 pubmed publisher
    ..Together with functional studies of other mutants, we conclude that the classical view of GTP hydrolysis mechanism likely remains the same in the HflX family with a twist in the entity of the nucleophilic alignment...
  11. Guilfoyle A, Maher M, Rapp M, Clarke R, Harrop S, Jormakka M. Structural basis of GDP release and gating in G protein coupled Fe2+ transport. EMBO J. 2009;28:2677-85 pubmed publisher
    ..From these results, structural parallels are drawn to eukaryotic G protein coupled membrane processes. ..
  12. Gromadski K, Schümmer T, Strømgaard A, Knudsen C, Kinzy T, Rodnina M. Kinetics of the interactions between yeast elongation factors 1A and 1Balpha, guanine nucleotides, and aminoacyl-tRNA. J Biol Chem. 2007;282:35629-37 pubmed
    ..eEF1A.GTP binds Phe-tRNA(Phe) with a K(d) of 3 nm, whereas eEF1A.GDP shows no significant binding, indicating that eEF1A has similar tRNA binding properties as its prokaryotic homolog, EF-Tu. ..
  13. Oreb M, Höfle A, Koenig P, Sommer M, Sinning I, Wang F, et al. Substrate binding disrupts dimerization and induces nucleotide exchange of the chloroplast GTPase Toc33. Biochem J. 2011;436:313-9 pubmed publisher
    ..We discuss this novel regulatory mode and its impact on the current models of protein import into the chloroplast. ..
  14. Guilfoyle A, Deshpande C, Vincent K, Pedroso M, Schenk G, Maher M, et al. Structural and functional analysis of a FeoB A143S G5 loop mutant explains the accelerated GDP release rate. FEBS J. 2014;281:2254-65 pubmed publisher
    ..We conclude that the identity of the residue at this position in the G5 loop plays a key role in the nucleotide release rate by allowing the correct positioning and hydrogen bonding of the nucleotide base. ..
  15. Yajima H, Ogura T, Nitta R, Okada Y, Sato C, Hirokawa N. Conformational changes in tubulin in GMPCPP and GDP-taxol microtubules observed by cryoelectron microscopy. J Cell Biol. 2012;198:315-22 pubmed publisher
    ..regulation remains elusive because high-resolution microtubule structures have only been revealed for the guanosine diphosphate (GDP) state...
  16. Paleskava A, Konevega A, Rodnina M. Thermodynamic and kinetic framework of selenocysteyl-tRNASec recognition by elongation factor SelB. J Biol Chem. 2010;285:3014-20 pubmed publisher
    ..The mode of tRNA recognition by SelB is reminiscent of another specialized factor, eIF2, rather than of EF-Tu, the common delivery factor for all other aminoacyl-tRNAs, in line with a common evolutionary ancestry of SelB and eIF2. ..
  17. Kiel C, Filchtinski D, Spoerner M, Schreiber G, Kalbitzer H, Herrmann C. Improved binding of raf to Ras.GDP is correlated with biological activity. J Biol Chem. 2009;284:31893-902 pubmed publisher
    ..In a luciferase-based reporter gene assay, Raf A85K is associated with higher signaling activity, which appears to be a mere matter of Ras-Raf affinity. ..
  18. Wypijewska A, Bojarska E, Lukaszewicz M, Stepinski J, Jemielity J, Davis R, et al. 7-methylguanosine diphosphate (m(7)GDP) is not hydrolyzed but strongly bound by decapping scavenger (DcpS) enzymes and potently inhibits their activity. Biochemistry. 2012;51:8003-13 pubmed publisher
    ..Our data have important implications for the regulatory role of m(7)GDP in mRNA metabolic pathways due to its possible interactions with different cap-binding proteins, such as DcpS or eIF4E...
  19. Tomar S, Dhimole N, Chatterjee M, Prakash B. Distinct GDP/GTP bound states of the tandem G-domains of EngA regulate ribosome binding. Nucleic Acids Res. 2009;37:2359-70 pubmed publisher
    ..Our results suggest a model where distinct nucleotide-bound states of the two G-domains regulate formation of specific EngA-ribosome complexes. ..
  20. Vellano C, Shu F, Ramineni S, Yates C, Tall G, Hepler J. Activation of the regulator of G protein signaling 14-G?i1-GDP signaling complex is regulated by resistance to inhibitors of cholinesterase-8A. Biochemistry. 2011;50:752-62 pubmed publisher
    ..These findings demonstrate that RGS14 is a newly appreciated integrator of unconventional Ric-8A and G?i1 signaling. ..
  21. Cook P, Holden H. GDP-perosamine synthase: structural analysis and production of a novel trideoxysugar. Biochemistry. 2008;47:2833-40 pubmed publisher
    ..Details describing the X-ray structural investigation of GDP-perosamine synthase and the enzymatic synthesis of GDP-4-amino-3,4,6-trideoxy- d-mannose are presented. ..
  22. Min M, Jang M, Lee M, Lee J, Song K, Lee Y, et al. Recruitment of Arf1-GDP to Golgi by Glo3p-type ArfGAPs is crucial for golgi maintenance and plant growth. Plant Physiol. 2013;161:676-91 pubmed publisher
    ..Based on these results, we propose that the Glo3p-type ArfGAPs AGD8 and AGD9 recruit Arf1-GDP from the cytosol to the Golgi for Arf1-mediated protein trafficking, which is essential for plant development and growth. ..
  23. Kopein D, Katanaev V. Drosophila GoLoco-protein Pins is a target of Galpha(o)-mediated G protein-coupled receptor signaling. Mol Biol Cell. 2009;20:3865-77 pubmed publisher
    ..g., in the context of the nervous system development, where Galpha(o) acts downstream from Frizzled and redundantly with Galpha(i) to control the asymmetry of cell divisions. ..
  24. Kubota M, Tanaka T, Kohno T, Wakamatsu K. GDP-GTP exchange processes of G{alpha}i1 protein are accelerated/decelerated depending on the type and the concentration of added detergents. J Biochem. 2009;146:875-80 pubmed publisher
    ..These effects of detergents on G-proteins must be taken into account in G-protein experiments. ..
  25. Shabalina I, Ost M, Petrovic N, Vrbacky M, Nedergaard J, Cannon B. Uncoupling protein-1 is not leaky. Biochim Biophys Acta. 2010;1797:773-84 pubmed publisher
  26. Paleskava A, Konevega A, Rodnina M. Thermodynamics of the GTP-GDP-operated conformational switch of selenocysteine-specific translation factor SelB. J Biol Chem. 2012;287:27906-12 pubmed publisher
    ..The similarity of the GTP and GDP forms in the crystal structures can be attributed to the use of GDPNP, which appears to induce a structure of SelB that is more similar to the GDP than to the GTP-bound form. ..
  27. Saito T, Nishi M, Lim M, Wu B, Maeda T, Hashimoto H, et al. A novel GDP-dependent pyruvate kinase isozyme from Toxoplasma gondii localizes to both the apicoplast and the mitochondrion. J Biol Chem. 2008;283:14041-52 pubmed publisher
    ..This is the first study of a potential mitochondrial pyruvate kinase in any system. ..
  28. Jeong J, Lee K, Kim Y, Kim D, Oh B, Kim Y. Crystal structure of the Gtr1p(GTP)-Gtr2p(GDP) protein complex reveals large structural rearrangements triggered by GTP-to-GDP conversion. J Biol Chem. 2012;287:29648-53 pubmed publisher
    ..These features explain how the nucleotide-bound statuses of the two GTPases subunits switch the Raptor binding affinity on and off. ..
  29. Hauryliuk V, Hansson S, Ehrenberg M. Cofactor dependent conformational switching of GTPases. Biophys J. 2008;95:1704-15 pubmed publisher
    ..The scheme is also used to discuss some problems of interpretation that may arise when guanine nucleotide exchange mechanisms are inferred from experiments with analogs of GTP, like GDPNP, GDPCP, and GDP gamma S. ..
  30. Mitkevich V, Ermakov A, Kulikova A, Tankov S, Shyp V, Soosaar A, et al. Thermodynamic characterization of ppGpp binding to EF-G or IF2 and of initiator tRNA binding to free IF2 in the presence of GDP, GTP, or ppGpp. J Mol Biol. 2010;402:838-46 pubmed publisher
  31. Lau S, Tanner M. Mechanism and active site residues of GDP-fucose synthase. J Am Chem Soc. 2008;130:17593-602 pubmed publisher
    ..Together these results strongly implicate an ordered sequence of epimerizations (C-3'' followed by C-5'') and suggest that Cys109 acts as a base and His179 acts as an acid in both epimerization steps. ..
  32. Schuette J, Murphy F, Kelley A, Weir J, Giesebrecht J, Connell S, et al. GTPase activation of elongation factor EF-Tu by the ribosome during decoding. EMBO J. 2009;28:755-65 pubmed publisher
    ..Thus, the structure provides insights into the molecular mechanism of signalling codon recognition from the decoding centre of the 30S subunit to the GTPase centre of EF-Tu. ..
  33. Johnson J, Erickson J, Cerione R. New insights into how the Rho guanine nucleotide dissociation inhibitor regulates the interaction of Cdc42 with membranes. J Biol Chem. 2009;284:23860-71 pubmed publisher
  34. Schoenhofen I, Vinogradov E, Whitfield D, Brisson J, Logan S. The CMP-legionaminic acid pathway in Campylobacter: biosynthesis involving novel GDP-linked precursors. Glycobiology. 2009;19:715-25 pubmed publisher
  35. Chun J, Shapovalova Z, Dejgaard S, Presley J, Melancon P. Characterization of class I and II ADP-ribosylation factors (Arfs) in live cells: GDP-bound class II Arfs associate with the ER-Golgi intermediate compartment independently of GBF1. Mol Biol Cell. 2008;19:3488-500 pubmed publisher
    ..Furthermore, they provide the first evidence that GBF1 accumulation on membranes may be caused by loss of Arf x GTP, rather than the formation of an Arf x GDP x BFA x GBF1 complex. ..
  36. Ford B, Boykevisch S, Zhao C, Kunzelmann S, Bar Sagi D, Herrmann C, et al. Characterization of a Ras mutant with identical GDP- and GTP-bound structures . Biochemistry. 2009;48:11449-57 pubmed publisher
    ..Our data suggest that flexibility at position 60 is required for proper Sos-catalyzed nucleotide exchange and that structural information is somehow shared among the switch regions and the different nucleotide binding motifs. ..
  37. Luo Y, Fischer J, Baessler O, Schrey A, Ungewiss J, Glinski M, et al. GDP-capture compound--a novel tool for the profiling of GTPases in pro- and eukaryotes by capture compound mass spectrometry (CCMS). J Proteomics. 2010;73:815-9 pubmed publisher
    ..The GDP-Capture Compound robustly captures a wide range of different GTPases from both systems and will be a valuable tool for the proteomic profiling of this important protein family. ..
  38. Preininger A, Funk M, Oldham W, Meier S, Johnston C, Adhikary S, et al. Helix dipole movement and conformational variability contribute to allosteric GDP release in Galphai subunits. Biochemistry. 2009;48:2630-42 pubmed publisher
    ..Combined with previous evidence in the literature, the structural analysis supports the critical role of electrostatics of the alpha5 helix dipole and overall conformational variability during nucleotide release. ..
  39. Linster C, Adler L, Webb K, Christensen K, Brenner C, Clarke S. A second GDP-L-galactose phosphorylase in arabidopsis en route to vitamin C. Covalent intermediate and substrate requirements for the conserved reaction. J Biol Chem. 2008;283:18483-92 pubmed publisher
  40. Grafmüller A, Voth G. Intrinsic bending of microtubule protofilaments. Structure. 2011;19:409-17 pubmed publisher
    ..There are no observable differences between the mesoscopic properties of the contacts in GTP and GDP-bound tubulin or the intradime and interdimer interfaces. ..
  41. Van Eps N, Preininger A, Alexander N, Kaya A, Meier S, Meiler J, et al. Interaction of a G protein with an activated receptor opens the interdomain interface in the alpha subunit. Proc Natl Acad Sci U S A. 2011;108:9420-4 pubmed publisher
    ..The interdomain opening is coupled to receptor binding via the C-terminal helix of G(?), the extension of which is a high-affinity receptor binding element. ..
  42. Persson M, Franzen S, Catrina S, Dallner G, Hansell P, Brismar K, et al. Coenzyme Q10 prevents GDP-sensitive mitochondrial uncoupling, glomerular hyperfiltration and proteinuria in kidneys from db/db mice as a model of type 2 diabetes. Diabetologia. 2012;55:1535-43 pubmed publisher
  43. Cassimeris L. Microtubule assembly: lattice GTP to the rescue. Curr Biol. 2009;19:R174-6 pubmed publisher
    ..These GTP-tubulin islands may act as stop signs or speed bumps, switching a shortening microtubule back into a growing state. ..
  44. Meyer S, Böhme S, Krüger A, Steinhoff H, Klare J, Wittinghofer A. Kissing G domains of MnmE monitored by X-ray crystallography and pulse electron paramagnetic resonance spectroscopy. PLoS Biol. 2009;7:e1000212 pubmed publisher
    ..They show the nucleotide-dependent dynamic movements of the G domains around two swivel positions relative to the rest of the protein, and they are of crucial importance for understanding the mechanistic principles of this GAD. ..
  45. Yatime L, Mechulam Y, Blanquet S, Schmitt E. Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states. Proc Natl Acad Sci U S A. 2007;104:18445-50 pubmed publisher
    ..The nucleotide state of aIF2 shown here is indicative of a similar mechanism in archaea. Finally, we consider the possibility that release of Pi takes place after e/aIF2gamma has been informed of e/aIF1 dissociation by e/aIF2beta...
  46. Berghaus C, Schwarten M, Heumann R, Stoll R. Sequence-specific 1H, 13C, and 15N backbone assignment of the GTPase rRheb in its GDP-bound form. Biomol NMR Assign. 2007;1:45-7 pubmed publisher
  47. Alexander N, Preininger A, Kaya A, Stein R, Hamm H, Meiler J. Energetic analysis of the rhodopsin-G-protein complex links the ?5 helix to GDP release. Nat Struct Mol Biol. 2014;21:56-63 pubmed publisher
    ..Changes in the ?6-?5 loop displace ?G. All of these movements lead to opening of the GDP-binding pocket. The model creates a roadmap for experimental studies of receptor-mediated G-protein activation. ..
  48. Lonhienne T, Forwood J, Marfori M, Robin G, Kobe B, Carroll B. Importin-beta is a GDP-to-GTP exchange factor of Ran: implications for the mechanism of nuclear import. J Biol Chem. 2009;284:22549-58 pubmed publisher
    ..The exchange is also inhibited by nuclear-transport factor-2 (NTF2). We suggest a mechanism for nuclear import, additional to the established RCC1 (Ran-guanine exchange factor)-dependent pathway that incorporates these results. ..
  49. Biou V, Aizel K, Roblin P, Thureau A, Jacquet E, Hansson S, et al. SAXS and X-ray crystallography suggest an unfolding model for the GDP/GTP conformational switch of the small GTPase Arf6. J Mol Biol. 2010;402:696-707 pubmed publisher
    ..Taken together, these experiments suggest an unfolding model for the nucleotide switch of Arf6 and shed new light on its biochemical differences with Arf1. ..
  50. Westfield G, Rasmussen S, Su M, Dutta S, DeVree B, Chung K, et al. Structural flexibility of the G alpha s alpha-helical domain in the beta2-adrenoceptor Gs complex. Proc Natl Acad Sci U S A. 2011;108:16086-91 pubmed publisher
    ..These data argue that the ?-helical domain undergoes a nucleotide-dependent transition from a flexible to a conformationally stabilized state. ..
  51. Vohlander Rasmussen L, Oliveira C, Pedersen J, Sperling Petersen H, Mortensen K. Structural transitions of translation initiation factor IF2 upon GDPNP and GDP binding in solution. Biochemistry. 2011;50:9779-87 pubmed publisher
    ..The structural transitions of IF2C upon GDPNP binding and following nucleotide hydrolysis support the concept of cofactor-dependent conformational switching rather than the classical model for GTPase activity. ..
  52. Elie Caille C, Severin F, Helenius J, Howard J, Muller D, Hyman A. Straight GDP-tubulin protofilaments form in the presence of taxol. Curr Biol. 2007;17:1765-70 pubmed
    ..We go on to show that taxol stabilizes microtubules by straightening the GDP protofilament and slowing down the transition of protofilaments from straight to a curved configuration. ..
  53. Kimura T, Kaneko Y, Yamada S, Ishihara H, Senda T, Iwamatsu A, et al. The GDP-dependent Rab27a effector coronin 3 controls endocytosis of secretory membrane in insulin-secreting cell lines. J Cell Sci. 2008;121:3092-8 pubmed publisher
    ..have identified the actin-bundling protein coronin 3 as a novel Rab27a effector that paradoxically bound guanosine diphosphate (GDP)-Rab27a in the pancreatic beta-cell line MIN6...
  54. Jakubik J, Janickova H, El Fakahany E, Dolezal V. Negative cooperativity in binding of muscarinic receptor agonists and GDP as a measure of agonist efficacy. Br J Pharmacol. 2011;162:1029-44 pubmed publisher
    ..We analysed the role of guanosine diphosphate (GDP) in the process of activation of the M? muscarinic acetylcholine receptor and provide evidence that ..
  55. Shabalina I, Hoeks J, Kramarova T, Schrauwen P, Cannon B, Nedergaard J. Cold tolerance of UCP1-ablated mice: a skeletal muscle mitochondria switch toward lipid oxidation with marked UCP3 up-regulation not associated with increased basal, fatty acid- or ROS-induced uncoupling or enhanced GDP effects. Biochim Biophys Acta. 2010;1797:968-80 pubmed publisher
  56. Kueh H, Mitchison T. Structural plasticity in actin and tubulin polymer dynamics. Science. 2009;325:960-3 pubmed publisher
    ..We refer to these phenomena as "structural plasticity" and discuss emerging evidence that they play a central role in polymer dynamics and function. ..
  57. Ho T, Merajver S, Lapiere C, Nusgens B, Deroanne C. RhoA-GDP regulates RhoB protein stability. Potential involvement of RhoGDIalpha. J Biol Chem. 2008;283:21588-98 pubmed publisher
    ..Our results highlight RhoGDIalpha-dependent cross-talks that regulate the stability of RhoGTPases. ..
  58. Zielinski T, Kimple A, Hutsell S, Koeff M, Siderovski D, Lowery R. Two Galpha(i1) rate-modifying mutations act in concert to allow receptor-independent, steady-state measurements of RGS protein activity. J Biomol Screen. 2009;14:1195-206 pubmed publisher
    ..However, measuring GAP activity is complicated by slow guanosine diphosphate (GDP) release from Galpha and lack of solution phase assays for detecting free GDP in the presence of ..
  59. Jennings M, Zhou Y, Mohammad Qureshi S, Bennett D, Pavitt G. eIF2B promotes eIF5 dissociation from eIF2*GDP to facilitate guanine nucleotide exchange for translation initiation. Genes Dev. 2013;27:2696-707 pubmed publisher
    ..We propose a new model to place eIF2B GDF function in the context of efficient eIF2 recycling and its regulation by eIF2 phosphorylation...
  60. Kapoor N, Menon S, Chauhan R, Sachdev P, Sakmar T. Structural evidence for a sequential release mechanism for activation of heterotrimeric G proteins. J Mol Biol. 2009;393:882-97 pubmed publisher
    ..Interestingly, this mechanistic model for heterotrimeric G protein activation is similar to that suggested for the activation of the plant small G protein Rop4 by RopGEF8. ..
  61. Hauryliuk V, Mitkevich V, Draycheva A, Tankov S, Shyp V, Ermakov A, et al. Thermodynamics of GTP and GDP binding to bacterial initiation factor 2 suggests two types of structural transitions. J Mol Biol. 2009;394:621-6 pubmed publisher
    ..Also, this transition is likely to protect hydrophobic groups from solvent, and its functional relevance is discussed. ..
  62. Ogino T, Yadav S, Banerjee A. Histidine-mediated RNA transfer to GDP for unique mRNA capping by vesicular stomatitis virus RNA polymerase. Proc Natl Acad Sci U S A. 2010;107:3463-8 pubmed publisher
  63. Ash M, Guilfoyle A, Clarke R, Guss J, Maher M, Jormakka M. Potassium-activated GTPase reaction in the G Protein-coupled ferrous iron transporter B. J Biol Chem. 2010;285:14594-602 pubmed publisher
    ..In addition, the accelerated hydrolysis rate opens up the possibility that FeoB might indeed function as an active transporter. ..
  64. Valiron O, Arnal I, Caudron N, Job D. GDP-tubulin incorporation into growing microtubules modulates polymer stability. J Biol Chem. 2010;285:17507-13 pubmed publisher
    ..quot; ..
  65. Stites E, Trampont P, Ma Z, Ravichandran K. Network analysis of oncogenic Ras activation in cancer. Science. 2007;318:463-7 pubmed
    ..This system-level analysis of the oncogenic Ras network provides new insights and potential therapeutic strategies. ..
  66. Mellidou I, Chagne D, Laing W, Keulemans J, Davey M. Allelic variation in paralogs of GDP-L-galactose phosphorylase is a major determinant of vitamin C concentrations in apple fruit. Plant Physiol. 2012;160:1613-29 pubmed publisher
    ..Interestingly, colocations were also found between MdDHAR3-3 and a stable QTL for browning in the cv Telamon parent, highlighting links between the redox status of the AsA pool and susceptibility to flesh browning. ..
  67. Simonson T, Satpati P. Simulating GTP:Mg and GDP:Mg with a simple force field: a structural and thermodynamic analysis. J Comput Chem. 2013;34:836-46 pubmed publisher
    ..It has no effect on the free energy of GDP binding to a GTPase. ..
  68. Scheerer P, Heck M, Goede A, Park J, Choe H, Ernst O, et al. Structural and kinetic modeling of an activating helix switch in the rhodopsin-transducin interface. Proc Natl Acad Sci U S A. 2009;106:10660-5 pubmed publisher
    ..GDP to the nucleotide-free R*xG protein complex that illustrates how alpha5 might act as a transmission rod to propagate the conformational change from the receptor-G protein interface to the nucleotide binding site. ..
  69. Dong X, Yang B, Li Y, Zhong C, Ding J. Molecular basis of the acceleration of the GDP-GTP exchange of human ras homolog enriched in brain by human translationally controlled tumor protein. J Biol Chem. 2009;284:23754-64 pubmed publisher
    ..These data collectively provide insights into the molecular mechanisms of how hTCTP interacts with hRheb and activates the mTORC1 pathway. ..