3 beta d glucosidase glucan endo 1


Summary: An endocellulase with specificity for the hydrolysis of 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans including laminarin, paramylon, and pachyman.

Top Publications

  1. Hurtado Guerrero R, Schüttelkopf A, Mouyna I, Ibrahim A, Shepherd S, Fontaine T, et al. Molecular mechanisms of yeast cell wall glucan remodeling. J Biol Chem. 2009;284:8461-9 pubmed publisher
  2. McGrath C, Vuong T, Wilson D. Site-directed mutagenesis to probe catalysis by a Thermobifida fusca beta-1,3-glucanase (Lam81A). Protein Eng Des Sel. 2009;22:375-82 pubmed publisher
    ..In addition, mutation of D424 to histidine altered the substrate specificity by increasing the rate of cleavage of mixed-linkage beta-glucan and carboxymethyl-cellulose, 60- and 16-fold, respectively, over the wild-type enzyme. ..
  3. Kumagai Y, Ojima T. Enzymatic properties and the primary structure of a beta-1,3-glucanase from the digestive fluid of the Pacific abalone Haliotis discus hannai. Comp Biochem Physiol B Biochem Mol Biol. 2009;154:113-20 pubmed publisher
  4. Koch M, Pillus L. The glucanosyltransferase Gas1 functions in transcriptional silencing. Proc Natl Acad Sci U S A. 2009;106:11224-9 pubmed publisher
  5. Gastebois A, Mouyna I, Simenel C, Clavaud C, Coddeville B, Delepierre M, et al. Characterization of a new beta(1-3)-glucan branching activity of Aspergillus fumigatus. J Biol Chem. 2010;285:2386-96 pubmed publisher
    ..fumigatus did not display any cell wall phenotype showing that these activities were not responsible for the construction of the branched beta(1-3)-glucans of the cell wall. ..
  6. Shi P, Yao G, Yang P, Li N, Luo H, Bai Y, et al. Cloning, characterization, and antifungal activity of an endo-1,3-beta-D: -glucanase from Streptomyces sp. S27. Appl Microbiol Biotechnol. 2010;85:1483-90 pubmed publisher
    ..These favorable properties make BglS27 a good candidate for utilization in biotechnological applications such as plant protection, feed, and food preservation. ..
  7. Genta F, Bragatto I, Terra W, Ferreira C. Purification, characterization and sequencing of the major beta-1,3-glucanase from the midgut of Tenebrio molitor larvae. Insect Biochem Mol Biol. 2009;39:861-74 pubmed publisher
    ..TLam efficiently lyses fungal cells, suggesting a role in making available walls and cell contents to digestion and in protecting the midgut from pathogen infections...
  8. Ippel J, Koutsopoulos S, Nabuurs S, van Berkel W, van der Oost J, van Mierlo C. NMR characterization of a 264-residue hyperthermostable endo-beta-1,3-glucanase. Biochem Biophys Res Commun. 2010;391:370-5 pubmed publisher
    ..The data obtained form the basis for elucidation of the molecular origins involved in conformational stability and biological activity of hyperthermophilic endo-beta-1,3-glucanases at extreme temperatures. ..
  9. Miyajima Y, Satoh K, Umeda Y, Makimura K. Quantitation of fungal DNA contamination in commercial zymolyase and lyticase used in the preparation of fungi. Nihon Ishinkin Gakkai Zasshi. 2009;50:259-62 pubmed
    ..0323 copies/unit for Lyticase. The observations regarding these enzyme products indicate that careful consideration of contaminating DNA included in the reagents used for molecular diagnostics is necessary. ..

More Information


  1. Sachivkina N, Kravtsov E, Vasilyeva E, Anokhina I, Dalin M. Study of antimycotic activity of lyticase. Bull Exp Biol Med. 2009;148:214-6 pubmed
  2. Baek K, Knödler A, Lee S, Zhang X, Orlando K, Zhang J, et al. Structure-function study of the N-terminal domain of exocyst subunit Sec3. J Biol Chem. 2010;285:10424-33 pubmed publisher
    ..Through its ability to bind the small GTPase Cdc42 and phospholipids, the PH domain of Sec3 functions as a coincidence detector at the plasma membrane. ..
  3. Li X, Du W, Zhao J, Zhang L, Zhu Z, Jiang L. The MAP kinase-activated protein kinase Rck2p regulates cellular responses to cell wall stresses, filamentation and virulence in the human fungal pathogen Candida albicans. FEMS Yeast Res. 2010;10:441-51 pubmed publisher
    ..albicans. Therefore, Rck2p regulates cellular responses to cell wall stresses, filamentation and virulence in the human fungal pathogen C. albicans. ..
  4. Gastebois A, Fontaine T, Latge J, Mouyna I. beta(1-3)Glucanosyltransferase Gel4p is essential for Aspergillus fumigatus. Eukaryot Cell. 2010;9:1294-8 pubmed publisher
    ..Like the other Gelps, it encodes a glycosylphosphatidylinositol (GPI)-anchored protein. In contrast to the other beta(1-3)glucanosyltransferases analyzed to date, it is essential for this fungal species. ..
  5. Backhaus K, Heilmann C, Sorgo A, Purschke G, de Koster C, Klis F, et al. A systematic study of the cell wall composition of Kluyveromyces lactis. Yeast. 2010;27:647-60 pubmed publisher
    ..Importantly, the composition of the cell wall glycoproteome depended on carbon source and growth phase. Our results clearly illustrate the dynamic nature of the cell wall of K. lactis and provide a firm base for studying its regulation...
  6. Lettner T, Zeidler U, Gimona M, Hauser M, Breitenbach M, Bito A. Candida albicans AGE3, the ortholog of the S. cerevisiae ARF-GAP-encoding gene GCS1, is required for hyphal growth and drug resistance. PLoS ONE. 2010;5:e11993 pubmed publisher
    ..albicans and essential for detoxification of azole drugs which are routinely used for antifungal therapy. Thus, it represents a promising antifungal drug target. ..