plant tumor inducing plasmids


Summary: Plasmids coding for proteins which induce PLANT TUMORS. The most notable example of a plant tumor inducing plasmid is the Ti plasmid found associated with AGROBACTERIUM TUMEFACIENS.

Top Publications

  1. Chen G, Malenkos J, Cha M, Fuqua C, Chen L. Quorum-sensing antiactivator TraM forms a dimer that dissociates to inhibit TraR. Mol Microbiol. 2004;52:1641-51 pubmed
    ..2002, EMBO J 21: 4393-4401), our structural analysis suggests overlapping interactive surfaces in homodimeric TraM with those in the TraM-TraR complex and a mechanism for TraM inhibition on TraR. ..
  2. Chai Y, Winans S. RepB protein of an Agrobacterium tumefaciens Ti plasmid binds to two adjacent sites between repA and repB for plasmid partitioning and autorepression. Mol Microbiol. 2005;58:1114-29 pubmed publisher
    ..We propose that RepA and RepB form complexes that bind both sites, possibly causing a loop that is important for repression of the repABC operon. Binding at one or both sites may also be required for accurate plasmid partitioning...
  3. Wise A, Fang F, Lin Y, He F, Lynn D, Binns A. The receiver domain of hybrid histidine kinase VirA: an enhancing factor for vir gene expression in Agrobacterium tumefaciens. J Bacteriol. 2010;192:1534-42 pubmed publisher
    ..These phenotypes were corrected if the virG copy number was increased. In addition, we show that the VirA receiver domain can interact with the VirG DNA-binding domain...
  4. Xu H, Zhou X, Lu J, Wang J, Wang X. Hairy roots induced by Agrobacterium rhizogenes and production of regenerative plants in hairy root cultures in maize. Sci China C Life Sci. 2006;49:305-10 pubmed
    ..The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA. The PCR-Southern hybridization demonstrated that T-DNA had been integrated into the chromosome of regenerated plants. ..
  5. Yukawa K, Kaku H, Tanaka H, Koga Ban Y, Fukuda M. Characterization and host range determination of soybean super virulent Agrobacterium tumefaciens KAT23. Biosci Biotechnol Biochem. 2007;71:1676-82 pubmed
    ..This virulent ability of KAT23 indicates its potential application to legumes, especially to soybean transformation. ..
  6. Nazar R, Robb E. Borrowing from biology. Science. 2007;318:565 pubmed
  7. Kiyokawa K, Yamamoto S, Sakuma K, Tanaka K, Moriguchi K, Suzuki K. Construction of disarmed Ti plasmids transferable between Escherichia coli and Agrobacterium species. Appl Environ Microbiol. 2009;75:1845-51 pubmed publisher
    ..As the modified plasmid delivery system was simple and efficient, conversion of strains to the disarmed type was easy and should be applicable in studies to screen for useful strains. ..
  8. Khan S, Farrand S. The BlcC (AttM) lactonase of Agrobacterium tumefaciens does not quench the quorum-sensing system that regulates Ti plasmid conjugative transfer. J Bacteriol. 2009;191:1320-9 pubmed publisher
    ..We conclude that BlcC has no biologically significant effect on Ti plasmid transfer or its regulatory system. ..
  9. Carlier A, Uroz S, Smadja B, Fray R, Latour X, Dessaux Y, et al. The Ti plasmid of Agrobacterium tumefaciens harbors an attM-paralogous gene, aiiB, also encoding N-Acyl homoserine lactonase activity. Appl Environ Microbiol. 2003;69:4989-93 pubmed
    ..In Erwinia strain 6276, the lactonases reduced the endogenous acyl-HSL level and the bacterial virulence in planta...

More Information


  1. Komari T, Takakura Y, Ueki J, Kato N, Ishida Y, Hiei Y. Binary vectors and super-binary vectors. Methods Mol Biol. 2006;343:15-41 pubmed
    ..Because no vector is perfect for every project, it is recommended that modification or construction of vectors should be made according to the objective of the experiments. Existing vectors serve as good sources of components. ..
  2. Qin Y, Smyth A, Su S, Farrand S. Dimerization properties of TraM, the antiactivator that modulates TraR-mediated quorum-dependent expression of the Ti plasmid tra genes. Mol Microbiol. 2004;53:1471-85 pubmed
    ..When co-expressed with lambda cI'::TraR fusions, wild-type TraM mediated quormone-independent dimerization of the transcriptional activator, suggesting that dimers of TraM can multimerize TraR. ..
  3. Volokhina I, Sazonova I, Velikov V, Chumakov M. Isolation, purification, and identification of the virulence protein VirE2 from Agrobacterium tumefaciens. Microbiol Res. 2005;160:67-73 pubmed
    ..Anti-VirE2 miniantibodies were obtained, and direct labeling of VirE2 with colloidal gold was done for the first time. ..
  4. Chai Y, Winans S. A small antisense RNA downregulates expression of an essential replicase protein of an Agrobacterium tumefaciens Ti plasmid. Mol Microbiol. 2005;56:1574-85 pubmed
    ..Sequence analysis suggests that this phenomenon may be widespread among plasmids of this family. ..
  5. Cho H, Winans S. TraA, TraC and TraD autorepress two divergent quorum-regulated promoters near the transfer origin of the Ti plasmid of Agrobacterium tumefaciens. Mol Microbiol. 2007;63:1769-82 pubmed
    ..TraA preferentially acted in cis in repressing the traA and traC promoters and in the processing of oriT, which explains the very high activity of plasmid-borne traA-lacZ fusions reported in previous studies. ..
  6. Zhiming R, Zheng M, Wei S, Huiying F, Jian Z. Transformation of industrialized strain Candida glycerinogenes with resistant gene zeocin via Agrobacterium tumefaciens. Curr Microbiol. 2008;57:12-7 pubmed publisher
    ..glycerologenesis cells. Our results demonstrated that A. tumefaciens-mediated transformation can be used for C. glycerinogenes. This transformation system can provide the basis for research of C. glycerologenesis in the future. ..
  7. Sakakibara H, Kasahara H, Ueda N, Kojima M, Takei K, Hishiyama S, et al. Agrobacterium tumefaciens increases cytokinin production in plastids by modifying the biosynthetic pathway in the host plant. Proc Natl Acad Sci U S A. 2005;102:9972-7 pubmed
    ..These results demonstrate that A. tumefaciens modifies CK biosynthesis by sending a key enzyme into plastids of the host plant to promote tumorigenesis. ..
  8. Schmidt Heydt M, Schunck T, Geisen R. Expression of a gfp gene in Penicillium nordicum under control of the promoter of the ochratoxin A polyketide synthase gene. Int J Food Microbiol. 2009;133:161-6 pubmed publisher
  9. Nair G, Liu Z, Binns A. Reexamining the role of the accessory plasmid pAtC58 in the virulence of Agrobacterium tumefaciens strain C58. Plant Physiol. 2003;133:989-99 pubmed
    ..Our studies indicate that pAtC58 has a positive effect on vir gene induction as revealed by immunoblot analysis of Vir proteins and expression of a PvirB::lacZ fusion. ..
  10. Toussaint A, Merlin C, Monchy S, Benotmane M, Leplae R, Mergeay M, et al. The biphenyl- and 4-chlorobiphenyl-catabolic transposon Tn4371, a member of a new family of genomic islands related to IncP and Ti plasmids. Appl Environ Microbiol. 2003;69:4837-45 pubmed
    ..Tn4371 thus appears to represent a new family of potentially mobile genomic islands with a broad host range since they reside in a wide range of soil proteobacteria, including plant pathogens. ..
  11. PuĊ‚awska J, Sobiczewski P. Development of a semi-nested PCR based method for sensitive detection of tumorigenic Agrobacterium in soil. J Appl Microbiol. 2005;98:710-21 pubmed
    ..The method is proposed for testing of soil in fields intended for nursery production of fruit trees, roses or other plants susceptible to crown gall, as well as a tool for ecological studies. ..
  12. Pruss G, Nester E, Vance V. Infiltration with Agrobacterium tumefaciens induces host defense and development-dependent responses in the infiltrated zone. Mol Plant Microbe Interact. 2008;21:1528-38 pubmed publisher
    ..tumefaciens. We found that C58-infiltrated zones-but not those infiltrated with the disarmed control-have increased levels of miR393, a microRNA that represses auxin signaling and contributes to antibacterial resistance. ..
  13. Pappas K. Cell-cell signaling and the Agrobacterium tumefaciens Ti plasmid copy number fluctuations. Plasmid. 2008;60:89-107 pubmed publisher
    ..It remains to be seen whether similar copy number variations characterize related replicons or if they are of even broader significance in plasmid biology. ..
  14. Michielse C, Ram A, Hooykaas P, Hondel C. Role of bacterial virulence proteins in Agrobacterium-mediated transformation of Aspergillus awamori. Fungal Genet Biol. 2004;41:571-8 pubmed
    ..The results indicate that the Agrobacterium-mediated transformation of A. awamori requires an intact T-DNA machinery for efficient transformation; however, the plant host range factors, like VirE3, VirH, and VirF, are not important. ..
  15. Cubero J, Lastra B, Salcedo C, Piquer J, Lopez M. Systemic movement of Agrobacterium tumefaciens in several plant species. J Appl Microbiol. 2006;101:412-21 pubmed
    ..The results show that migration of Agrobacterium spp. could be relatively frequent in several cultivated fruit trees, and systemic infections should be taken into account when designing strategies for controlling crown gall disease. ..
  16. Yamamoto S, Uraji M, Tanaka K, Moriguchi K, Suzuki K. Identification of pTi-SAKURA DNA region conferring enhancement of plasmid incompatibility and stability. Genes Genet Syst. 2007;82:197-206 pubmed
    ..These data suggest that tiorf24 and tiorf25 are responsible for the stability of pTi-SAKURA, and reduce, in the host bacterium, the frequency of ejection of the resident plasmid, presumably through an incompatibility mechanism. ..
  17. Qin Y, Su S, Farrand S. Molecular basis of transcriptional antiactivation. TraM disrupts the TraR-DNA complex through stepwise interactions. J Biol Chem. 2007;282:19979-91 pubmed
    ..From these genetic and structural considerations, we constructed three-dimensional models of the complex that shed light on the mechanism of TraM-mediated inhibition of TraR and on TraM-mediated destabilization of the TraR-DNA complex. ..
  18. Cho H, Pinto U, Winans S. Transsexuality in the rhizosphere: quorum sensing reversibly converts Agrobacterium tumefaciens from phenotypically female to male. J Bacteriol. 2009;191:3375-83 pubmed publisher
    ..In the presence of these signals, these strains block the entry of Ti plasmids and instead become efficient conjugal donors. ..
  19. Shao J, Long Y, Chen G, Xie J, Xu Z. The reversed terminator of octopine synthase gene on the Agrobacterium Ti plasmid has a weak promoter activity in prokaryotes. Mol Biol Rep. 2010;37:2157-62 pubmed publisher
    ..tumefaciens, which overlaps in reverse with the coding region of the OCS gene in octopine Ti plasmid. ..
  20. Kado C. Negative transcriptional regulation of virulence and oncogenes of the Ti plasmid by Ros bearing a conserved C2H2-zinc finger motif. Plasmid. 2002;48:179-85 pubmed
    ..Interestingly, ros homologues were found in microorganisms derived from marine sources, supporting the hypothesis that ros may have originated from a marine rather than a terrestrial organism. ..
  21. Gelvin S. Agrobacterium virulence gene induction. Methods Mol Biol. 2006;343:77-84 pubmed
    ..This chapter discusses conditions for efficient induction of Agrobacterium virulence genes by phenolic compounds. ..
  22. Lee L. Integration of genes into the chromosome of Agrobacterium tumefaciens C58. Methods Mol Biol. 2006;343:55-66 pubmed
    ..The integrated gene is stably maintained as single copy per cell without the need for selection. ..
  23. Howe A, Sato S, Dweikat I, Fromm M, Clemente T. Rapid and reproducible Agrobacterium-mediated transformation of sorghum. Plant Cell Rep. 2006;25:784-91 pubmed
    ..Seed set for the primary transgenic plants ranged from 145 to 1400 seed/plant. Analysis of T(1) progeny demonstrated transmission of the transgenes in a simple Mendelian fashion in the majority of events. ..
  24. Flowers J, Vaillancourt L. Parameters affecting the efficiency of Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola. Curr Genet. 2005;48:380-8 pubmed
    ..Thus, ATMT may be unsuitable for insertional mutagenesis of C. graminicola without further modification. ..
  25. Udagawa M, Aoki S, Syono K. Expression analysis of the NgORF13 promoter during the development of tobacco genetic tumors. Plant Cell Physiol. 2004;45:1023-31 pubmed
    ..1996) Plant Cell Physiol. 37: 489-498. It is thought that Ngrol genes might be involved in the formation of genetic tumors, and, moreover, NgORF13 might work in cooperation with NgrolB and NgrolC. ..
  26. Pappas K, Winans S. The RepA and RepB autorepressors and TraR play opposing roles in the regulation of a Ti plasmid repABC operon. Mol Microbiol. 2003;49:441-55 pubmed
    ..These findings demonstrate that both autoinduction and autorepression play critical and opposing roles in regulating repABC expression and hence in the replication, stability and copy number of the Ti plasmid. ..
  27. Wise A, Liu Z, Binns A. Nucleic acid extraction from Agrobacterium strains. Methods Mol Biol. 2006;343:67-76 pubmed
    ..In addition, we provide a simple method for the electronic transfer of shuttle plasmids from Agrobacterium to E. coli for use when their low copy number in Agrobacterium impedes plasmid isolation from that strain. ..
  28. Terakura S, Kitakura S, Ishikawa M, Ueno Y, Fujita T, Machida C, et al. Oncogene 6b from Agrobacterium tumefaciens induces abaxial cell division at late stages of leaf development and modifies vascular development in petioles. Plant Cell Physiol. 2006;47:664-72 pubmed
  29. Su S, Khan S, Farrand S. Induction and loss of Ti plasmid conjugative competence in response to the acyl-homoserine lactone quorum-sensing signal. J Bacteriol. 2008;190:4398-407 pubmed publisher
  30. Khan S, Gaines J, Roop R, Farrand S. Broad-host-range expression vectors with tightly regulated promoters and their use to examine the influence of TraR and TraM expression on Ti plasmid quorum sensing. Appl Environ Microbiol. 2008;74:5053-62 pubmed publisher
    ..We also used this system to show that the antiactivator TraM sets the level of intracellular TraR required for tra gene activation. ..
  31. Rommens C, Bougri O, Yan H, Humara J, Owen J, Swords K, et al. Plant-derived transfer DNAs. Plant Physiol. 2005;139:1338-49 pubmed
    ..New insights in border region requirements were used to construct an all-native alfalfa (Medicago sativa) transfer DNA vector that can be used for the production of intragenic plants. ..
  32. Teyssier Cuvelle S, Oger P, Mougel C, Groud K, Farrand S, Nesme X. A highly selectable and highly transferable Ti plasmid to study conjugal host range and Ti plasmid dissemination in complex ecosystems. Microb Ecol. 2004;48:10-8 pubmed
    ..g., the presence of incompatible plasmids. As a consequence, a species should be considered permissive to Ti transfer whenever one permissive isolate is found. ..
  33. Uraji M, Suzuki K, Yoshida K. A novel plasmid curing method using incompatibility of plant pathogenic Ti plasmids in Agrobacterium tumefaciens. Genes Genet Syst. 2002;77:1-9 pubmed
    ..rhizogenes, by replacing the rep gene region of the curing plasmid with that of the corresponding incompatibility. ..
  34. Gelvin S. Plant proteins involved in Agrobacterium-mediated genetic transformation. Annu Rev Phytopathol. 2010;48:45-68 pubmed publisher
  35. Cho H, Winans S. VirA and VirG activate the Ti plasmid repABC operon, elevating plasmid copy number in response to wound-released chemical signals. Proc Natl Acad Sci U S A. 2005;102:14843-8 pubmed
    ..Mutating this sequence blocked the AS-induced copy number increase of a RepABC-dependent miniplasmid, indicating that phospho-VirG increases copy number solely by enhancing repABC expression. ..