homologous recombination

Summary

Summary: An exchange of DNA between matching or similar sequences.

Top Publications

  1. Shay J, Reddel R, Wright W. Cancer. Cancer and telomeres--an ALTernative to telomerase. Science. 2012;336:1388-90 pubmed publisher
  2. Takayama K, Igai K, Hagihara Y, Hashimoto R, Hanawa M, Sakuma T, et al. Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system. Nucleic Acids Res. 2017;45:5198-5207 pubmed publisher
    ..To enhance the biallelic homologous recombination efficiency in human ES/iPS cells, we performed screenings of accessorial genes and compounds...
  3. Allen C, Hirakawa H, Nakajima N, Moore S, Nie J, Sharma N, et al. Low- and High-LET Ionizing Radiation Induces Delayed Homologous Recombination that Persists for Two Weeks before Resolving. Radiat Res. 2017;188:82-93 pubmed publisher
    ..Delayed effects include hypermutation, hyper-homologous recombination, chromosome instability and reduced clonogenic survival (delayed death)...
  4. Sun Y, Zhang X, Wu C, He Y, Ma Y, Hou H, et al. Engineering Herbicide-Resistant Rice Plants through CRISPR/Cas9-Mediated Homologous Recombination of Acetolactate Synthase. Mol Plant. 2016;9:628-31 pubmed publisher
  5. Lux C, Scharenberg A. Therapeutic Gene Editing Safety and Specificity. Hematol Oncol Clin North Am. 2017;31:787-795 pubmed publisher
    ..It is important to plot a safe course for future clinical trials. This article reviews safety and specificity for therapeutic gene editing to spur dialogue and advancement. ..
  6. Kawazu M, Kojima S, Ueno T, Totoki Y, Nakamura H, Kunita A, et al. Integrative analysis of genomic alterations in triple-negative breast cancer in association with homologous recombination deficiency. PLoS Genet. 2017;13:e1006853 pubmed publisher
    ..Silencing of the BRCA1 gene impaired the homologous recombination pathway in a subset of TNBCs, which exhibited similar phenotypes to tumors with BRCA1 mutations; they ..
  7. Morgan A, Gatti D, Najarian M, Keane T, Galante R, Pack A, et al. Structural Variation Shapes the Landscape of Recombination in Mouse. Genetics. 2017;206:603-619 pubmed publisher
    ..Our findings demonstrate how multiparent populations, by bridging the gap between large-scale and fine-scale genetic mapping, can reveal new features of the recombination landscape. ..
  8. Lee B, Singh A, Mark Glover J, Hendzel M, Spyracopoulos L. Molecular Basis for K63-Linked Ubiquitination Processes in Double-Strand DNA Break Repair: A Focus on Kinetics and Dynamics. J Mol Biol. 2017;429:3409-3429 pubmed publisher
    ..break (DSB), which is detected and repaired by mechanisms known as non-homologous end-joining (NHEJ) and homologous recombination (HR), which are components of the DNA damage response system...
  9. Watkins J, Irshad S, Grigoriadis A, Tutt A. Genomic scars as biomarkers of homologous recombination deficiency and drug response in breast and ovarian cancers. Breast Cancer Res. 2014;16:211 pubmed publisher
    ..or somatic mutations in the BRCA1 or BRCA2 genes, the products of which contribute to the conservative homologous recombination repair of DNA double-strand breaks...

More Information

Publications66

  1. Tessé S, Bourbon H, Debuchy R, Budin K, Dubois E, Liangran Z, et al. Asy2/Mer2: an evolutionarily conserved mediator of meiotic recombination, pairing, and global chromosome compaction. Genes Dev. 2017;31:1880-1893 pubmed publisher
    ..Thus, beyond its role as a recombinosome-axis/SC linker molecule, Mer2 has important functions in relation to basic chromosome structure. ..
  2. Killock D. Targeted therapies: DNA polymerase ?-a new target for synthetic lethality?. Nat Rev Clin Oncol. 2015;12:125 pubmed publisher
  3. Su H, Cheng Z, Huang J, Lin J, Copenhaver G, Ma H, et al. Arabidopsis RAD51, RAD51C and XRCC3 proteins form a complex and facilitate RAD51 localization on chromosomes for meiotic recombination. PLoS Genet. 2017;13:e1006827 pubmed publisher
    ..Finally, we hypothesize that maintenance of RAD51 function facilitated by the RAD51C-XRCC3 complex could be highly conserved in eukaryotes. ..
  4. Wallace N, Khanal S, Robinson K, Wendel S, Messer J, Galloway D. High-Risk Alphapapillomavirus Oncogenes Impair the Homologous Recombination Pathway. J Virol. 2017;91: pubmed publisher
    ..Our data support this idea since HPV E6 stimulates the integration of episomes. ..
  5. Yuan C, Liu W, Wang Y, Hou J, Zhang L, Wang G. Homologous recombination is a force in the evolution of canine distemper virus. PLoS ONE. 2017;12:e0175416 pubmed publisher
    ..Collectively, these data suggested that homologous recombination acts as a key force driving the genetic diversity and evolution of canine distemper virus.
  6. Alligand B, Le Breton M, Marquis D, Vallette F, Fleury F. Functional effects of diphosphomimetic mutations at cAbl-mediated phosphorylation sites on Rad51 recombinase activity. Biochimie. 2017;139:115-124 pubmed publisher
    b>Homologous Recombination enables faithful repair of the deleterious double strand breaks of DNA. This pathway relies on Rad51 to catalyze homologous DNA strand exchange...
  7. Singh G, Da Ines O, Gallego M, White C. Analysis of the impact of the absence of RAD51 strand exchange activity in Arabidopsis meiosis. PLoS ONE. 2017;12:e0183006 pubmed publisher
    ..In this work we confirm and extend this conclusion. Given that more than 95% of meiotic homologous recombination in Arabidopsis does not result in inter-homologue crossovers, Arabidopsis is a particularly sensitive ..
  8. Shibata A. Regulation of repair pathway choice at two-ended DNA double-strand breaks. Mutat Res. 2017;803-805:51-55 pubmed publisher
    ..Non-homologous end joining (NHEJ) functions in G1/S/G2 phase, while homologous recombination (HR) becomes active only in S/G2 phase after DNA replication...
  9. Dubois J, Yates M, Gaudreau Lapierre A, Clement G, Cappadocia L, Gaudreau L, et al. A phosphorylation-and-ubiquitylation circuitry driving ATR activation and homologous recombination. Nucleic Acids Res. 2017;45:8859-8872 pubmed publisher
    ..DNA (RPA-ssDNA), a nucleoprotein structure induced by DNA damage, promotes ATR activation and homologous recombination (HR). RPA is hyper-phosphorylated and ubiquitylated after DNA damage...
  10. Takata K, Reh S, Yousefzadeh M, Zelazowski M, Bhetawal S, Trono D, et al. Analysis of DNA polymerase ν function in meiotic recombination, immunoglobulin class-switching, and DNA damage tolerance. PLoS Genet. 2017;13:e1006818 pubmed publisher
    ..Our results suggest a function of pol ν in meiotic homologous recombination in processing specific substrates...
  11. Dalia T, Yoon S, Galli E, Barre F, Waters C, Dalia A. Enhancing multiplex genome editing by natural transformation (MuGENT) via inactivation of ssDNA exonucleases. Nucleic Acids Res. 2017;45:7527-7537 pubmed publisher
    ..Thus, rational removal of ssDNA exonucleases may be broadly applicable for enhancing the efficacy and ease of MuGENT in diverse naturally transformable species. ..
  12. Zelensky A, Schimmel J, Kool H, Kanaar R, Tijsterman M. Inactivation of Pol ? and C-NHEJ eliminates off-target integration of exogenous DNA. Nat Commun. 2017;8:66 pubmed publisher
    ..In contrast, homologous recombination is not reduced in these cells and gene targeting is improved to 100% efficiency...
  13. Nath S, Somyajit K, Mishra A, Scully R, Nagaraju G. FANCJ helicase controls the balance between short- and long-tract gene conversions between sister chromatids. Nucleic Acids Res. 2017;45:8886-8900 pubmed publisher
    ..Although FANCJ has been implicated in the repair of DNA double-strand breaks (DSBs) by homologous recombination (HR), the molecular mechanism underlying the tumor suppressor functions of FANCJ remains obscure...
  14. de Kok S, Stanton L, Slaby T, Durot M, Holmes V, Patel K, et al. Rapid and reliable DNA assembly via ligase cycling reaction. ACS Synth Biol. 2014;3:97-106 pubmed publisher
    ..Yeast homologous recombination and LCR both enabled reliable assembly of up to 12 DNA parts with 60-100% of individual clones being ..
  15. Zhu X, Keeney S. Zip it up to shut it down. Cell Cycle. 2014;13:2157-8 pubmed publisher
  16. Symington L. DNA repair: Making the cut. Nature. 2014;514:39-40 pubmed publisher
  17. Mijic S, Zellweger R, Chappidi N, Berti M, Jacobs K, Mutreja K, et al. Replication fork reversal triggers fork degradation in BRCA2-defective cells. Nat Commun. 2017;8:859 pubmed publisher
    Besides its role in homologous recombination, the tumor suppressor BRCA2 protects stalled replication forks from nucleolytic degradation...
  18. Beaudet D, Terrat Y, Halary S, de la Providencia I, Hijri M. Mitochondrial genome rearrangements in glomus species triggered by homologous recombination between distinct mtDNA haplotypes. Genome Biol Evol. 2013;5:1628-43 pubmed publisher
    ..However, these elements may also lead to genome rearrangements through homologous recombination, although this has never previously been reported in this group of obligate symbiotic fungi...
  19. Kevorkian Y, Shen A. Revisiting the Role of Csp Family Proteins in Regulating Clostridium difficile Spore Germination. J Bacteriol. 2017;199: pubmed publisher
    ..Our data also suggest that a subpopulation of C. difficile spores spontaneously germinates in the absence of bile salt germinants and/or Csp proteins. ..
  20. Jasin Mansur F, Takahara S, Yamamoto M, Shimatani M, Minnatul Karim M, Noiri Y, et al. Purification and characterization of hemolysin from periodontopathogenic bacterium Eikenella corrodens strain 1073. Biosci Biotechnol Biochem. 2017;81:1246-1253 pubmed publisher
    ..Meanwhile, a strain with a disrupted hlyA gene, which was constructed by homologous recombination, did not show any hemolytic activity...
  21. Vilaine M, Olcaydu D, Harutyunyan A, Bergeman J, Tiab M, Ramée J, et al. Homologous recombination of wild-type JAK2, a novel early step in the development of myeloproliferative neoplasm. Blood. 2011;118:6468-70 pubmed publisher
  22. Ukai H, Kiyonari H, Ueda H. Production of knock-in mice in a single generation from embryonic stem cells. Nat Protoc. 2017;12:2513-2530 pubmed publisher
    ..In this report, we describe the basic technologies and protocols for this procedure, and discuss the current challenges for next-generation mammalian genetics in organism-level systems biology studies...
  23. Kong Y, Ma J, Warren K, Tsang R, Low D, Jamieson F, et al. Homologous recombination drives both sequence diversity and gene content variation in Neisseria meningitidis. Genome Biol Evol. 2013;5:1611-27 pubmed publisher
    ..identical clonal complexes (CCs) in Nm has been recently reported and suggested to be the result of homologous recombination, but the extent to which recombination contributes to genomic variation within identical CCs has remained ..
  24. Lu W, Hawley B, Skalka G, Baldock R, Smith E, Bader A, et al. Drosha drives the formation of DNA:RNA hybrids around DNA break sites to facilitate DNA repair. Nat Commun. 2018;9:532 pubmed publisher
    ..Depletion of Drosha significantly reduces DNA repair by both homologous recombination (HR) and non-homologous end joining (NHEJ)...
  25. Bouabe H, Moser M, Heesemann J. Enhanced selection for homologous-recombinant embryonic stem cell clones by Cre recombinase-mediated deletion of the positive selection marker. Transgenic Res. 2012;21:227-9 pubmed publisher
  26. David S, Sánchez Busó L, Harris S, Marttinen P, Rusniok C, Buchrieser C, et al. Dynamics and impact of homologous recombination on the evolution of Legionella pneumophila. PLoS Genet. 2017;13:e1006855 pubmed publisher
    ..pneumophila, particularly with regards to homologous recombination (whereby genes are replaced with alternative allelic variants)...
  27. Chu D, Gromova T, Newman T, Burgess S. The Nucleoporin Nup2 Contains a Meiotic-Autonomous Region that Promotes the Dynamic Chromosome Events of Meiosis. Genetics. 2017;206:1319-1337 pubmed publisher
    ..These data suggest Nup2 and Ndj1 support partially overlapping functions that promote two different levels of meiotic chromosome organization necessary to withstand a dynamic stage of the eukaryotic life cycle. ..
  28. Kohn D. Historical Perspective on the Current Renaissance for Hematopoietic Stem Cell Gene Therapy. Hematol Oncol Clin North Am. 2017;31:721-735 pubmed publisher
    ..Gene editing using site-specific endonucleases is emerging as a promising technology for gene therapy and is moving into clinical trials. ..
  29. Göhring A, Reuter S, Clement J, Cheng X, Theobald J, Wolfl S, et al. Human microRNA-299-3p decreases invasive behavior of cancer cells by downregulation of Oct4 expression and causes apoptosis. PLoS ONE. 2017;12:e0174912 pubmed publisher
    ..The results suggest that microRNA-299-3p is an interesting target for potential clinical use. It may be able to decrease invasive behaviour of carcinoma cells; or even kill these cells by causing apoptosis. ..
  30. Shao S, Ren C, Liu Z, Bai Y, Chen Z, Wei Z, et al. Enhancing CRISPR/Cas9-mediated homology-directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52. Int J Biochem Cell Biol. 2017;92:43-52 pubmed publisher
    ..determined that the HDR efficiency could be enhanced by expressing Rad52, a gene that is involved in the homologous recombination process...
  31. Pokhrel N, Origanti S, Davenport E, Gandhi D, Kaniecki K, Mehl R, et al. Monitoring Replication Protein A (RPA) dynamics in homologous recombination through site-specific incorporation of non-canonical amino acids. Nucleic Acids Res. 2017;45:9413-9426 pubmed publisher
    ..of facilitated self-exchange and exchange by Rad51 and mediator proteins during various stages in homologous recombination. RPAf is widely applicable to investigate its mechanism of action in processes such as DNA replication, ..
  32. Hernández G, Ramírez M, Minguillón J, Quiles P, Ruiz de Garibay G, Aza Carmona M, et al. Decapping protein EDC4 regulates DNA repair and phenocopies BRCA1. Nat Commun. 2018;9:967 pubmed publisher
    BRCA1 is a tumor suppressor that regulates DNA repair by homologous recombination. Germline mutations in BRCA1 are associated with increased risk of breast and ovarian cancer and BRCA1 deficient tumors are exquisitely sensitive to poly (..
  33. Rong Z, Zhu S, Xu Y, Fu X. Homologous recombination in human embryonic stem cells using CRISPR/Cas9 nickase and a long DNA donor template. Protein Cell. 2014;5:258-60 pubmed publisher
  34. Gravells P, Grant E, Smith K, James D, Bryant H. Specific killing of DNA damage-response deficient cells with inhibitors of poly(ADP-ribose) glycohydrolase. DNA Repair (Amst). 2017;52:81-91 pubmed publisher
    ..We confirm that PARG inhibition increases endogenous DNA damage, stalls replication forks and increases homologous recombination, and propose that it is the lack of homologous recombination (HR) proteins at PARG inhibitor-induced ..
  35. Natsume T, Nishimura K, Minocherhomji S, Bhowmick R, Hickson I, Kanemaki M. Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. 2017;31:816-829 pubmed publisher
    ..We show that MCM8-9 functions in a homologous recombination-based pathway downstream from RAD51, which is promoted by DSB induction...
  36. Schwartz C, Frogue K, Ramesh A, Misa J, Wheeldon I. CRISPRi repression of nonhomologous end-joining for enhanced genome engineering via homologous recombination in Yarrowia lipolytica. Biotechnol Bioeng. 2017;114:2896-2906 pubmed publisher
    In many organisms of biotechnological importance precise genome editing is limited by inherently low homologous recombination (HR) efficiencies...
  37. Wilson D, Rieckher M, Williams A, Schumacher B. Systematic analysis of DNA crosslink repair pathways during development and aging in Caenorhabditis elegans. Nucleic Acids Res. 2017;45:9467-9480 pubmed publisher
    ..The analysis also revealed contributions of homologous recombination (BRC-1/BRCA1), the MUS-81, EXO-1, SLX-1 and FAN-1 nucleases, and the DOG-1 (FANCJ) helicase in ICL ..
  38. Butler J, Santos R, Martens G, Ladowski J, Wang Z, Li P, et al. Efficient generation of targeted and controlled mutational events in porcine cells using nuclease-directed homologous recombination. J Surg Res. 2017;212:238-245 pubmed publisher
    ..The object of this study was to use nonintegrating selection and nuclease-directed homologous recombination to efficiently control the genetic modification of the porcine genome...
  39. Zhang F, Tang H, Jiang Y, Mao Z. The transcription factor GATA3 is required for homologous recombination repair by regulating CtIP expression. Oncogene. 2017;36:5168-5176 pubmed publisher
    ..In summary, our data delineates the regulatory mechanisms of GATA3 in DNA DSB repair and strongly suggests that it might act as a tumor suppressor by promoting CtIP expression and HR to stabilize genomes. ..
  40. Chen H, Yang P, Guo J, Kwoh C, Przytycka T, Zheng J. ARG-walker: inference of individual specific strengths of meiotic recombination hotspots by population genomics analysis. BMC Genomics. 2015;16 Suppl 12:S1 pubmed publisher
    ..In the future, it could be used to uncover the mechanisms of recombination regulation and human diseases related with recombination hotspots. ..
  41. Sulkowski P, Corso C, Robinson N, Scanlon S, Purshouse K, Bai H, et al. 2-Hydroxyglutarate produced by neomorphic IDH mutations suppresses homologous recombination and induces PARP inhibitor sensitivity. Sci Transl Med. 2017;9: pubmed publisher
    ..We report that IDH1/2 mutations induce a homologous recombination (HR) defect that renders tumor cells exquisitely sensitive to poly(adenosine 5'-diphosphate-ribose) ..
  42. Marchesini M, Ogoti Y, Fiorini E, Aktaş Samur A, Nezi L, D Anca M, et al. ILF2 Is a Regulator of RNA Splicing and DNA Damage Response in 1q21-Amplified Multiple Myeloma. Cancer Cell. 2017;32:88-100.e6 pubmed publisher
    ..splicing factor U2AF65, which promotes mRNA processing and the stabilization of transcripts involved in homologous recombination in response to DNA damage...
  43. Li X, Baker J, Cracknell T, Haynes A, Blanco G. IGFN1_v1 is required for myoblast fusion and differentiation. PLoS ONE. 2017;12:e0180217 pubmed publisher
    ..Targeting of N-terminal domains by elimination of exon 13 via CRISPR/Cas9 mediated homologous recombination, also resulted in fusion defects as well as large multinucleated cells...
  44. Lang A, Westbye A, Beatty J. The Distribution, Evolution, and Roles of Gene Transfer Agents in Prokaryotic Genetic Exchange. Annu Rev Virol. 2017;4:87-104 pubmed publisher
  45. Al Sweel N, Raghavan V, Dutta A, Ajith V, Di Vietro L, Khondakar N, et al. mlh3 mutations in baker's yeast alter meiotic recombination outcomes by increasing noncrossover events genome-wide. PLoS Genet. 2017;13:e1006974 pubmed publisher
    ..They also illustrate how defective meiotic components can alter the fate of meiotic recombination intermediates, providing new insights for how meiotic recombination pathways are regulated. ..
  46. Nickoloff J, Jones D, Lee S, Williamson E, Hromas R. Drugging the Cancers Addicted to DNA Repair. J Natl Cancer Inst. 2017;109: pubmed publisher
    ..In addition, future therapies will exploit artificial synthetic lethality, where complementary DNA repair pathways are targeted simultaneously in cancers without DNA repair defects. ..
  47. Dai J, Voloshin O, Potapova S, Camerini Otero R. Meiotic Knockdown and Complementation Reveals Essential Role of RAD51 in Mouse Spermatogenesis. Cell Rep. 2017;18:1383-1394 pubmed publisher
    Meiotic homologous recombination (HR) is important for proper chromosomal segregation during gametogenesis and facilitates evolutionary adaptation via genomic reshuffling...
  48. Gualberto J, Newton K. Plant Mitochondrial Genomes: Dynamics and Mechanisms of Mutation. Annu Rev Plant Biol. 2017;68:225-252 pubmed publisher
    ..They are involved mainly in pathways of DNA repair by homologous recombination and mismatch repair, which appear to be essential for the faithful replication of the mitogenome...
  49. Carzaniga T, Sbarufatti G, Briani F, Dehò G. Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli. BMC Microbiol. 2017;17:81 pubmed publisher
    ..In this work we present evidence that in Escherichia coli PNPase enhances both homologous recombination upon P1 transduction and error prone DNA repair of double strand breaks induced by zeocin, a radiomimetic ..
  50. Tiang C, He Y, Pawlowski W. Chromosome organization and dynamics during interphase, mitosis, and meiosis in plants. Plant Physiol. 2012;158:26-34 pubmed publisher
  51. Brion C, Legrand S, Peter J, Caradec C, Pflieger D, Hou J, et al. Variation of the meiotic recombination landscape and properties over a broad evolutionary distance in yeasts. PLoS Genet. 2017;13:e1006917 pubmed publisher
    ..Identification of this major but underestimated evolutionary phenomenon illustrates the relevance of exploring non-model species. ..
  52. Fiedler M, Gensheimer T, Kubisch C, Meyer V. HisB as novel selection marker for gene targeting approaches in Aspergillus niger. BMC Microbiol. 2017;17:57 pubmed publisher
    ..niger, hisB, which allows efficient homologous integration rates as well as high expression levels which compare favorably to the well-established pyrG selection marker. ..
  53. Bacon L, Hamley Bennett C, Danson M, Leak D. Development of an efficient technique for gene deletion and allelic exchange in Geobacillus spp. Microb Cell Fact. 2017;16:58 pubmed publisher
    ..Here we describe a new technique to create marker-less mutations in Geobacillus utilising a novel homologous recombination process...
  54. Zhao Y, Dominska M, Petrova A, Bagshaw H, Kokoska R, Petes T. Properties of Mitotic and Meiotic Recombination in the Tandemly-Repeated CUP1 Gene Cluster in the Yeast Saccharomyces cerevisiae. Genetics. 2017;206:785-800 pubmed publisher
    ..In summary, our study shows that some of the basic properties of recombination differ considerably between single-copy and tandemly-repeated genes. ..
  55. Argunhan B, Murayama Y, Iwasaki H. The differentiated and conserved roles of Swi5-Sfr1 in homologous recombination. FEBS Lett. 2017;591:2035-2047 pubmed publisher
    b>Homologous recombination (HR) is the process whereby two DNA molecules that share high sequence similarity are able to recombine to generate hybrid DNA molecules...
  56. Zerbini F, Zanella I, Fraccascia D, König E, Irene C, Frattini L, et al. Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli. Microb Cell Fact. 2017;16:68 pubmed publisher
    The exploitation of the CRISPR/Cas9 machinery coupled to lambda (?) recombinase-mediated homologous recombination (recombineering) is becoming the method of choice for genome editing in E. coli...
  57. Penha R, Lima S, Boroni M, Ramalho Oliveira R, Viola J, de Carvalho D, et al. Intrinsic LINE-1 Hypomethylation and Decreased Brca1 Expression are Associated with DNA Repair Delay in Irradiated Thyroid Cells. Radiat Res. 2017;188:144-155 pubmed publisher
    ..the impact of ionizing radiation on global methylation and CpG islands within promoter regions of homologous recombination (HR) and non-homologous end joining (NHEJ) genes, as well as its effects on gene expression, using two ..