anaphase

Summary

Summary: The phase of cell nucleus division following METAPHASE, in which the CHROMATIDS separate and migrate to opposite poles of the spindle.

Top Publications

  1. Lianga N, Williams E, Kennedy E, Dore C, Pilon S, Girard S, et al. A Wee1 checkpoint inhibits anaphase onset. J Cell Biol. 2013;201:843-62 pubmed publisher
    Cdk1 drives both mitotic entry and the metaphase-to-anaphase transition. Past work has shown that Wee1 inhibition of Cdk1 blocks mitotic entry...
  2. Bieling P, Telley I, Surrey T. A minimal midzone protein module controls formation and length of antiparallel microtubule overlaps. Cell. 2010;142:420-32 pubmed publisher
    ..In anaphase, this antiparallel microtubule array is tightly bundled forming the midzone, which serves as a hub for the ..
  3. Dotiwala F, Eapen V, Harrison J, Arbel Eden A, Ranade V, Yoshida S, et al. DNA damage checkpoint triggers autophagy to regulate the initiation of anaphase. Proc Natl Acad Sci U S A. 2013;110:E41-9 pubmed publisher
    ..double-strand break (DSB) trigger the Mec1 (ATR)-dependent DNA damage response that causes them to arrest before anaphase for 12-15 h...
  4. Galimberti F, Thompson S, Liu X, Li H, Memoli V, Green S, et al. Targeting the cyclin E-Cdk-2 complex represses lung cancer growth by triggering anaphase catastrophe. Clin Cancer Res. 2010;16:109-20 pubmed publisher
    ..Induction of multipolar anaphases leading to anaphase catastrophe is a previously unrecognized mechanism engaged by targeting the cyclin E-Cdk-2 complex...
  5. Baldinger T, Gossen M. Binding of Drosophila ORC proteins to anaphase chromosomes requires cessation of mitotic cyclin-dependent kinase activity. Mol Cell Biol. 2009;29:140-9 pubmed publisher
    ..DmOrc2-GFP is not detected on metaphase chromosomes but binds rapidly to anaphase chromatin in Drosophila embryos...
  6. Brust Mascher I, Sommi P, Cheerambathur D, Scholey J. Kinesin-5-dependent poleward flux and spindle length control in Drosophila embryo mitosis. Mol Biol Cell. 2009;20:1749-62 pubmed publisher
    ..activity maintains the prometaphase spindle by antagonizing Ncd and another unknown force-generator and drives anaphase B, although the rate of spindle elongation is relatively insensitive to the motor's concentration...
  7. Park I, Park K, Park J, Chung W. Isoliquiritigenin induces G2 and M phase arrest by inducing DNA damage and by inhibiting the metaphase/anaphase transition. Cancer Lett. 2009;277:174-81 pubmed publisher
    ..The induction of DNA damage and blockade at the metaphase/anaphase transition implied that isoliquiritigenin might function as a topoisomerase II poison, which was further ..
  8. Hashash N, Johnson A, Cha R. Topoisomerase II- and condensin-dependent breakage of MEC1ATR-sensitive fragile sites occurs independently of spindle tension, anaphase, or cytokinesis. PLoS Genet. 2012;8:e1002978 pubmed publisher
    ..including errors in replication fork restart, premature mitotic chromosome condensation, spindle tension, anaphase, and cytokinesis...
  9. Meadows J, Millar J. Latrunculin A delays anaphase onset in fission yeast by disrupting an Ase1-independent pathway controlling mitotic spindle stability. Mol Biol Cell. 2008;19:3713-23 pubmed publisher
    It has been proposed previously that latrunculin A, an inhibitor of actin polymerization, delays the onset of anaphase by causing spindle misorientation in fission yeast...

Scientific Experts

More Information

Publications62

  1. Hancioglu B, Tyson J. A mathematical model of mitotic exit in budding yeast: the role of Polo kinase. PLoS ONE. 2012;7:e30810 pubmed publisher
    ..of mitotic kinases (Cdk and Polo kinases) and phosphatases (Cdc55/PP2A and Cdc14), as well as the action of the anaphase promoting complex (APC) in degrading specific proteins in anaphase and telophase...
  2. Chan K, Hickson I. On the origins of ultra-fine anaphase bridges. Cell Cycle. 2009;8:3065-6 pubmed
  3. Gardner M, Haase J, Mythreye K, Molk J, Anderson M, Joglekar A, et al. The microtubule-based motor Kar3 and plus end-binding protein Bim1 provide structural support for the anaphase spindle. J Cell Biol. 2008;180:91-100 pubmed publisher
    ..Upon anaphase onset, kMTs shorten to the pole, whereas ipMTs increase in length...
  4. Matos I, Pereira A, Lince Faria M, Cameron L, Salmon E, Maiato H. Synchronizing chromosome segregation by flux-dependent force equalization at kinetochores. J Cell Biol. 2009;186:11-26 pubmed publisher
    The synchronous movement of chromosomes during anaphase ensures their correct inheritance in every cell division. This reflects the uniformity of spindle forces acting on chromosomes and their simultaneous entry into anaphase...
  5. Buttrick G, Meadows J, Lancaster T, Vanoosthuyse V, Shepperd L, Hoe K, et al. Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B. Mol Biol Cell. 2011;22:4486-502 pubmed publisher
    ..Nsk1 localizes to the interface between kinetochores and the inner face of the spindle pole body during anaphase. In the absence of Nsk1, some kinetochores become detached from spindle poles during anaphase B...
  6. H garat N, Smith E, Nayak G, Takeda S, Eyers P, Hochegger H. Aurora A and Aurora B jointly coordinate chromosome segregation and anaphase microtubule dynamics. J Cell Biol. 2011;195:1103-13 pubmed publisher
    ..Conversely, cells lacking AurA and AurB activity exit mitosis without anaphase, forming polyploid daughter cells with a single nucleus...
  7. Spence J, Phua H, Mills W, Carpenter A, Porter A, Farr C. Depletion of topoisomerase IIalpha leads to shortening of the metaphase interkinetochore distance and abnormal persistence of PICH-coated anaphase threads. J Cell Sci. 2007;120:3952-64 pubmed
  8. Voets E, Wolthuis R. MASTL is the human orthologue of Greatwall kinase that facilitates mitotic entry, anaphase and cytokinesis. Cell Cycle. 2010;9:3591-601 pubmed
    ..RNAi cells that enter and progress through mitosis often fail to completely separate their sister chromatids in anaphase. This causes chromatin to be trapped in the cleavage furrow, which may lead to the formation of 4N G(1) cells by ..
  9. Floyd S, Pines J, Lindon C. APC/C Cdh1 targets aurora kinase to control reorganization of the mitotic spindle at anaphase. Curr Biol. 2008;18:1649-58 pubmed publisher
    Control of mitotic cell cycles by the anaphase-promoting complex or cyclosome (APC/C) ubiquitin ligase depends on its coactivators Cdc20 and Cdh1...
  10. Fuller B, Lampson M, Foley E, Rosasco Nitcher S, Le K, Tobelmann P, et al. Midzone activation of aurora B in anaphase produces an intracellular phosphorylation gradient. Nature. 2008;453:1132-6 pubmed publisher
    ..The anaphase array of microtubules that self-organize at the spindle midzone contributes to positioning the cell-division ..
  11. Hartl T, Sweeney S, Knepler P, Bosco G. Condensin II resolves chromosomal associations to enable anaphase I segregation in Drosophila male meiosis. PLoS Genet. 2008;4:e1000228 pubmed publisher
    ..In mutants of either subunit, territory formation fails and chromatin is dispersed throughout the nucleus. Anaphase I is also abnormal in Cap-H2 mutants as chromatin bridges are found between segregating heterologous and ..
  12. Cabernard C, Prehoda K, Doe C. A spindle-independent cleavage furrow positioning pathway. Nature. 2010;467:91-4 pubmed publisher
    ..early and late furrow proteins (Pavarotti, Anillin, and Myosin) are localized to the neuroblast basal cortex at anaphase onset by a Pins cortical polarity pathway, and can induce a basally displaced furrow even in the complete absence ..
  13. Hu D, Wan Y. Regulation of Krüppel-like factor 4 by the anaphase promoting complex pathway is involved in TGF-beta signaling. J Biol Chem. 2011;286:6890-901 pubmed publisher
    ..We have identified the Cdh1-anaphase promoting complex as a putative E3 ligase that governs TGF-?-induced KLF4 degradation...
  14. Rath U, Sharp D. The molecular basis of anaphase A in animal cells. Chromosome Res. 2011;19:423-32 pubmed publisher
    The mechanisms that move chromatids poleward during anaphase A have fascinated researchers for decades...
  15. Harrison B, Hoang M, Bloom K. Persistent mechanical linkage between sister chromatids throughout anaphase. Chromosoma. 2009;118:633-45 pubmed publisher
    ..Upon anaphase, these arm loci are drawn into the spindle, where they adopt a cruciform-like structure and stretch 2...
  16. Goto G, Mishra A, Abdulle R, Slaughter C, Kitagawa K. Bub1-mediated adaptation of the spindle checkpoint. PLoS Genet. 2011;7:e1001282 pubmed publisher
    ..accurate chromosome segregation by monitoring the kinetochore-microtubule interaction and delaying the onset of anaphase until each pair of sister chromosomes is properly attached to microtubules...
  17. Li H, Chen Q, Kaller M, Nellen W, Gräf R, De Lozanne A. Dictyostelium Aurora kinase has properties of both Aurora A and Aurora B kinases. Eukaryot Cell. 2008;7:894-905 pubmed publisher
    ..B, DdAurora binds to its partner DdINCENP and localizes on centromeres at metaphase, the central spindle during anaphase, and the cleavage furrow at the end of cytokinesis. DdAurora also has several unusual properties...
  18. Zhang T, Nirantar S, Lim H, Sinha I, Surana U. DNA damage checkpoint maintains CDH1 in an active state to inhibit anaphase progression. Dev Cell. 2009;17:541-51 pubmed publisher
    ..This control circuit involves Rad53, polo kinase, the anaphase-promoting complex activator Cdh1, and the bimC kinesin family proteins Cin8 and Kip1...
  19. Vorozhko V, Emanuele M, Kallio M, Stukenberg P, Gorbsky G. Multiple mechanisms of chromosome movement in vertebrate cells mediated through the Ndc80 complex and dynein/dynactin. Chromosoma. 2008;117:169-79 pubmed
    ..of chromosomes on prometaphase spindles but failed to support metaphase alignment and chromatid movement in anaphase. Thus, in prometaphase, dynein/dynactin likely mediates early transient, lateral interactions of kinetochores and ..
  20. He E, Kapuy O, Oliveira R, Uhlmann F, Tyson J, Novak B. System-level feedbacks make the anaphase switch irreversible. Proc Natl Acad Sci U S A. 2011;108:10016-21 pubmed publisher
    ..checkpoint prevents a eukaryotic cell from commencing to separate its replicated genome into two daughter cells (anaphase) until all of its chromosomes are properly aligned on the metaphase plate, with the two copies of each chromosome ..
  21. Avunie Masala R, Movshovich N, Nissenkorn Y, Gerson Gurwitz A, Fridman V, Kõivomägi M, et al. Phospho-regulation of kinesin-5 during anaphase spindle elongation. J Cell Sci. 2011;124:873-8 pubmed publisher
    ..The kinesin-5 Saccharomyces cerevisiae homologue Cin8 is shown here to be differentially phosphorylated during late anaphase at Cdk1-specific sites located in its motor domain...
  22. Wang H, Brust Mascher I, Civelekoglu Scholey G, Scholey J. Patronin mediates a switch from kinesin-13-dependent poleward flux to anaphase B spindle elongation. J Cell Biol. 2013;203:35-46 pubmed publisher
    b>Anaphase B spindle elongation contributes to chromosome segregation during Drosophila melanogaster embryo mitosis...
  23. Afshar K, Werner M, Tse Y, Glotzer M, GONCZY P. Regulation of cortical contractility and spindle positioning by the protein phosphatase 6 PPH-6 in one-cell stage C. elegans embryos. Development. 2010;137:237-47 pubmed publisher
    ..we demonstrate that PPH-6/SAPS-1 is required for the proper generation of pulling forces on spindle poles during anaphase. Our results indicate that this requirement is distinct from the role in organizing the cortical actomyosin ..
  24. Pérez Benavente B, Garcia J, Rodriguez M, Pineda Lucena A, Piechaczyk M, Font de Mora J, et al. GSK3-SCF(FBXW7) targets JunB for degradation in G2 to preserve chromatid cohesion before anaphase. Oncogene. 2013;32:2189-99 pubmed publisher
  25. Harmey D, Smith A, Simanski S, Moussa C, Ayad N. The anaphase promoting complex induces substrate degradation during neuronal differentiation. J Biol Chem. 2009;284:4317-23 pubmed publisher
    The anaphase promoting complex (APC) is an E3 ubiquitin ligase required for the metaphase-to-anaphase transition and mitotic exit...
  26. O Connell C, Loncarek J, Hergert P, Kourtidis A, Conklin D, Khodjakov A. The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes. J Cell Biol. 2008;183:29-36 pubmed publisher
    ..Thus, kinetochore attachment to microtubules is sufficient to satisfy the SAC even in the absence of interkinetochore tension. ..
  27. Shcheprova Z, Baldi S, Frei S, Gonnet G, Barral Y. A mechanism for asymmetric segregation of age during yeast budding. Nature. 2008;454:728-34 pubmed publisher
    ..Our data involve septin-dependent diffusion barriers in the confinement of ageing factors to one daughter cell during asymmetric cell division. ..
  28. Sun L, Gao J, Dong X, Liu M, Li D, Shi X, et al. EB1 promotes Aurora-B kinase activity through blocking its inactivation by protein phosphatase 2A. Proc Natl Acad Sci U S A. 2008;105:7153-8 pubmed publisher
    ..Immunofluorescence microscopy shows that these two proteins colocalize on the central spindle in anaphase and in the midbody during cytokinesis...
  29. Zich J, Sochaj A, Syred H, Milne L, Cook A, Ohkura H, et al. Kinase activity of fission yeast Mph1 is required for Mad2 and Mad3 to stably bind the anaphase promoting complex. Curr Biol. 2012;22:296-301 pubmed publisher
    ..The spindle checkpoint delays anaphase onset until all chromosomes are attached to spindle microtubules in a bipolar fashion [3, 4]...
  30. Rouzeau S, Cordelières F, Buhagiar Labarchède G, Hurbain I, Onclercq Delic R, Gemble S, et al. Bloom's syndrome and PICH helicases cooperate with topoisomerase II? in centromere disjunction before anaphase. PLoS ONE. 2012;7:e33905 pubmed publisher
    ..Centromeric ultrafine anaphase bridges are physiological DNA structures thought to contain unresolved DNA catenations between the centromeres ..
  31. Rock J, Amon A. Cdc15 integrates Tem1 GTPase-mediated spatial signals with Polo kinase-mediated temporal cues to activate mitotic exit. Genes Dev. 2011;25:1943-54 pubmed publisher
    ..the accurate execution of mitosis, MEN activity is coordinated with other cellular events and restricted to anaphase. The MEN GTPase Tem1 has been assumed to be the central switch in MEN regulation...
  32. Tarailo Graovac M, Chen N. Proper cyclin B3 dosage is important for precision of metaphase-to-anaphase onset timing in Caenorhabditis elegans. G3 (Bethesda). 2012;2:865-71 pubmed publisher
    ..CYB-3 (Cyclin B3) is a B-type cyclin that has been recently shown to be required for the timely metaphase-to-anaphase transition, presumably by alleviating a spindle assembly checkpoint (SAC) block...
  33. Wang L, Mayer B, Stemmann O, Nigg E. Centromere DNA decatenation depends on cohesin removal and is required for mammalian cell division. J Cell Sci. 2010;123:806-13 pubmed publisher
    ..The recent visualization of PICH (Plk1-interacting checkpoint helicase)-coated DNA threads in anaphase cells raises new questions as to the role of DNA catenation and its regulation in time and space...
  34. Zich J, Hardwick K. Getting down to the phosphorylated 'nuts and bolts' of spindle checkpoint signalling. Trends Biochem Sci. 2010;35:18-27 pubmed publisher
    ..Specifically, it delays anaphase onset until all chromosomes are attached in a bi-oriented fashion to spindle microtubules...
  35. Uehara R, Tsukada Y, Kamasaki T, Poser I, Yoda K, Gerlich D, et al. Aurora B and Kif2A control microtubule length for assembly of a functional central spindle during anaphase. J Cell Biol. 2013;202:623-36 pubmed publisher
    The central spindle is built during anaphase by coupling antiparallel microtubules (MTs) at a central overlap zone, which provides a signaling scaffold for the regulation of cytokinesis...
  36. Renshaw M, Ward J, Kanemaki M, Natsume K, Nedelec F, Tanaka T. Condensins promote chromosome recoiling during early anaphase to complete sister chromatid separation. Dev Cell. 2010;19:232-44 pubmed publisher
    Sister chromatid separation is initiated at anaphase onset by the activation of separase, which removes cohesins from chromosomes. However, it remains elusive how sister chromatid separation is completed along the entire chromosome length...
  37. Roubinet C, Decelle B, Chicanne G, Dorn J, Payrastre B, Payre F, et al. Molecular networks linked by Moesin drive remodeling of the cell cortex during mitosis. J Cell Biol. 2011;195:99-112 pubmed publisher
    ..phosphatase restricts high Moesin activity to early mitosis and down-regulates Moesin at the polar cortex, after anaphase onset...
  38. LaFountain J, Cohan C, Oldenbourg R. Functional states of kinetochores revealed by laser microsurgery and fluorescent speckle microscopy. Mol Biol Cell. 2011;22:4801-8 pubmed publisher
    ..In spermatocytes, elastic tethers connect telomeres of homologues during anaphase A of meiosis I, thus preventing complete disjunction until mid- to late anaphase A...
  39. Drapkin B, Lu Y, Procko A, Timney B, Cross F. Analysis of the mitotic exit control system using locked levels of stable mitotic cyclin. Mol Syst Biol. 2009;5:328 pubmed publisher
    Cyclin-dependent kinase (Cdk) both promotes mitotic entry (spindle assembly and anaphase) and inhibits mitotic exit (spindle disassembly and cytokinesis), leading to an elegant quantitative hypothesis that a single cyclin oscillation can ..
  40. Vader G, Lens S. The Aurora kinase family in cell division and cancer. Biochim Biophys Acta. 2008;1786:60-72 pubmed publisher
  41. Li Z, Umeyama T, Wang C. The Aurora Kinase in Trypanosoma brucei plays distinctive roles in metaphase-anaphase transition and cytokinetic initiation. PLoS Pathog. 2009;5:e1000575 pubmed publisher
    ..or metaphase invariably arrests the cells in the metaphase, suggesting an action of TbAUK1 in promoting metaphase-anaphase transition...
  42. Wang L, Schwarzbraun T, Speicher M, Nigg E. Persistence of DNA threads in human anaphase cells suggests late completion of sister chromatid decatenation. Chromosoma. 2008;117:123-35 pubmed
    ..to localize to kinetochores, inner centromeres, and thin threads connecting separating chromosomes even during anaphase. In this paper, we have used immuno-fiber fluorescence in situ hybridization and chromatin-immunoprecipitation to ..
  43. Khmelinskii A, Schiebel E. Assembling the spindle midzone in the right place at the right time. Cell Cycle. 2008;7:283-6 pubmed
    During anaphase the mitotic spindle extends dramatically, promoting the segregation of the chromosomes into the two daughter cells. The spindle midzone, assembled at the onset of anaphase, is critical for this extension...
  44. Kim E, Burke D. DNA damage activates the SAC in an ATM/ATR-dependent manner, independently of the kinetochore. PLoS Genet. 2008;4:e1000015 pubmed publisher
    ..Here we show that yeast cells lacking the DNA damage checkpoint arrest prior to anaphase in response to low doses of the DNA damaging agent methyl methane sulfonate (MMS)...
  45. Neurohr G, Naegeli A, Titos I, Theler D, Greber B, Diez J, et al. A midzone-based ruler adjusts chromosome compaction to anaphase spindle length. Science. 2011;332:465-8 pubmed publisher
    ..The spindle midzone, aurora/Ipl1 activity, and Ser10 of histone H3 mediated this response. Thus, the anaphase spindle may function as a ruler to adapt the condensation of chromatids, promoting their segregation regardless ..
  46. Akiyoshi B, Biggins S. Cdc14-dependent dephosphorylation of a kinetochore protein prior to anaphase in Saccharomyces cerevisiae. Genetics. 2010;186:1487-91 pubmed publisher
    ..Although Cdc14 activity is thought to be restricted to anaphase, we found that dephosphorylation of the Dsn1 kinetochore protein in metaphase requires Cdc14...
  47. Yoshida S, Bartolini S, Pellman D. Mechanisms for concentrating Rho1 during cytokinesis. Genes Dev. 2009;23:810-23 pubmed publisher
    ..We find that budding yeast utilizes two mechanisms to concentrate Rho1 at the division site. During anaphase, the primary mechanism for recruiting Rho1 is binding to its guanine nucleotide exchange factors (GEFs)...
  48. Herzog F, Primorac I, Dube P, Lenart P, Sander B, Mechtler K, et al. Structure of the anaphase-promoting complex/cyclosome interacting with a mitotic checkpoint complex. Science. 2009;323:1477-81 pubmed publisher
    Once all chromosomes are connected to the mitotic spindle (bioriented), anaphase is initiated by the protein ubiquitylation activity of the anaphase-promoting complex/cyclosome (APC/C) and its coactivator Cdc20 (APC/C(Cdc20))...
  49. Sebestova J, Danylevska A, Novakova L, Kubelka M, Anger M. Lack of response to unaligned chromosomes in mammalian female gametes. Cell Cycle. 2012;11:3011-8 pubmed publisher
    ..known that abolishing SAC increases frequency of chromosome segregation errors and causes precocious entry into anaphase; SAC, therefore, seems to be essential for normal chromosome segregation in meiosis I...
  50. Ahonen L, Kukkonen A, Pouwels J, Bolton M, Jingle C, Stukenberg P, et al. Perturbation of Incenp function impedes anaphase chromatid movements and chromosomal passenger protein flux at centromeres. Chromosoma. 2009;118:71-84 pubmed publisher
    ..Introduction of anti-Incenp antibody into early anaphase cells causes abnormalities in sister chromatid separation through defects in anaphase spindle functions...
  51. Vázquez Novelle M, Mirchenko L, Uhlmann F, Petronczki M. The 'anaphase problem': how to disable the mitotic checkpoint when sisters split. Biochem Soc Trans. 2010;38:1660-6 pubmed publisher
    ..The mitotic checkpoint monitors the attachment of kinetochores to microtubules and delays anaphase onset until all sister kinetochores have become attached to opposite poles...
  52. Sakurai H, Okado M, Ito F, Kawasaki K. Anaphase DNA bridges induced by lack of RecQ5 in Drosophila syncytial embryos. FEBS Lett. 2011;585:1923-8 pubmed publisher
    ..We demonstrate that the mitotic defects are derived from anaphase DNA bridges...
  53. Chow J, Poon R, Ma H. Inhibitory phosphorylation of cyclin-dependent kinase 1 as a compensatory mechanism for mitosis exit. Mol Cell Biol. 2011;31:1478-91 pubmed publisher
    The current paradigm states that exit from mitosis is triggered by the ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C) acting in concert with an activator called CDC20...