rough endoplasmic reticulum

Summary

Summary: A type of endoplasmic reticulum (ER) where polyribosomes are present on the cytoplasmic surfaces of the ER membranes. This form of ER is prominent in cells specialized for protein secretion and its principal function is to segregate proteins destined for export or intracellular utilization.

Top Publications

  1. Perreault S, Bousquet O, Lauzon M, Paiement J, Leclerc N. Increased association between rough endoplasmic reticulum membranes and mitochondria in transgenic mice that express P301L tau. J Neuropathol Exp Neurol. 2009;68:503-14 pubmed publisher
    ..More numerous contacts between rough endoplasmic reticulum (RER) membranes and mitochondria were observed in JNLP3 mice compared with wild-type mice...
  2. MacKenzie J, Westaway E. Assembly and maturation of the flavivirus Kunjin virus appear to occur in the rough endoplasmic reticulum and along the secretory pathway, respectively. J Virol. 2001;75:10787-99 pubmed
    ..site for flaviviruses in currently not known but is presumed to be located within the lumen of the rough endoplasmic reticulum (RER)...
  3. Ishikawa Y, Vranka J, Wirz J, Nagata K, Bachinger H. The rough endoplasmic reticulum-resident FK506-binding protein FKBP65 is a molecular chaperone that interacts with collagens. J Biol Chem. 2008;283:31584-90 pubmed publisher
    The rough endoplasmic reticulum-resident FK-506-binding protein FKBP65 can be isolated from chick embryos on a gelatin-Sepharose column, indicating some involvement in the biosynthesis of procollagens...
  4. Corsi A, Schekman R. Mechanism of polypeptide translocation into the endoplasmic reticulum. J Biol Chem. 1996;271:30299-302 pubmed
  5. Vranka J, Mokashi A, Keene D, Tufa S, Corson G, Sussman M, et al. Selective intracellular retention of extracellular matrix proteins and chaperones associated with pseudoachondroplasia. Matrix Biol. 2001;20:439-50 pubmed
  6. Rolls M, Hall D, Victor M, Stelzer E, Rapoport T. Targeting of rough endoplasmic reticulum membrane proteins and ribosomes in invertebrate neurons. Mol Biol Cell. 2002;13:1778-91 pubmed
    ..Ribosomes were also concentrated in the cell body, suggesting they may be in part responsible for targeting RER membrane proteins...
  7. Hecht J, Hayes E, Snuggs M, Decker G, Montufar Solis D, Doege K, et al. Calreticulin, PDI, Grp94 and BiP chaperone proteins are associated with retained COMP in pseudoachondroplasia chondrocytes. Matrix Biol. 2001;20:251-62 pubmed
    ..These results suggest that these proteins play a role in the processing and transport of wild type COMP in normal chondrocytes and in the retention of mutant COMP in PSACH chondrocytes...
  8. Ohashi S, Koike K, Omori A, Ichinose S, Ohara S, Kobayashi S, et al. Identification of mRNA/protein (mRNP) complexes containing Puralpha, mStaufen, fragile X protein, and myosin Va and their association with rough endoplasmic reticulum equipped with a kinesin motor. J Biol Chem. 2002;277:37804-10 pubmed
    ..Furthermore, mRNPs appear to reside on rough endoplasmic reticulum equipped with a kinesin motor...
  9. Ribeiro C, Vignes M, Brehelin M. Xenorhabdus nematophila (enterobacteriacea) secretes a cation-selective calcium-independent porin which causes vacuolation of the rough endoplasmic reticulum and cell lysis. J Biol Chem. 2003;278:3030-9 pubmed
    ..However, the size of the pores created by alpha X on macrophage or red blood cell plasma membrane increases with toxin concentration, which leads to a rapid cell lysis...

More Information

Publications62

  1. Lisenbee C, Heinze M, Trelease R. Peroxisomal ascorbate peroxidase resides within a subdomain of rough endoplasmic reticulum in wild-type Arabidopsis cells. Plant Physiol. 2003;132:870-82 pubmed
  2. Sepehri H, Eliassi A, Sauve R, Ashrafpour M, Saghiri R. Evidence for a large conductance voltage gated cationic channel in rough endoplasmic reticulum of rat hepatocytes. Arch Biochem Biophys. 2007;457:35-40 pubmed
    In this work, we report the single channel characterization of a voltage gated cationic channel from rough endoplasmic reticulum (RER) membranes of rat hepatocytes incorporated into a planar lipid bilayer...
  3. Wickham L, Duchaine T, Luo M, Nabi I, DesGroseillers L. Mammalian staufen is a double-stranded-RNA- and tubulin-binding protein which localizes to the rough endoplasmic reticulum. Mol Cell Biol. 1999;19:2220-30 pubmed
    ..Immunofluorescent double labeling of transfected mammalian cells revealed that Stau is localized to the rough endoplasmic reticulum (RER), implicating this RNA-binding protein in mRNA targeting to the RER, perhaps via a multistep ..
  4. Marion R, Fortes P, Beloso A, Dotti C, Ortin J. A human sequence homologue of Staufen is an RNA-binding protein that is associated with polysomes and localizes to the rough endoplasmic reticulum. Mol Cell Biol. 1999;19:2212-9 pubmed
    ..Colocalization was observed with the rough endoplasmic reticulum but not with endosomes, cytoskeleton, or Golgi apparatus...
  5. Oyanagi K, Yamazaki M, Takahashi H, Watabe K, Wada M, Komori T, et al. Spinal anterior horn cells in sporadic amyotrophic lateral sclerosis show ribosomal detachment from, and cisternal distention of the rough endoplasmic reticulum. Neuropathol Appl Neurobiol. 2008;34:650-8 pubmed publisher
    ..system in motor neurones of the brainstem and spinal cord, such as a decrease of cytoplasmic RNA and rough endoplasmic reticulum (rER) (chromatolysis), defective editing of the Q/R site of the glutamate receptor subunit GluR2 mRNA, ..
  6. Sardet C, Nishida H, Prodon F, Sawada K. Maternal mRNAs of PEM and macho 1, the ascidian muscle determinant, associate and move with a rough endoplasmic reticulum network in the egg cortex. Development. 2003;130:5839-49 pubmed
    ..cortices from eggs and zygotes we demonstrated that this reticulated structure is a network of cortical rough endoplasmic reticulum (cER) that is tethered to the plasma membrane...
  7. Ashrafpour M, Eliassi A, Sauve R, Sepehri H, Saghiri R. ATP regulation of a large conductance voltage-gated cation channel in rough endoplasmic reticulum of rat hepatocytes. Arch Biochem Biophys. 2008;471:50-6 pubmed publisher
    ..In this work we report the effect of ATP and ADP-Mg on a K+ channel present in the membrane of rough endoplasmic reticulum (RER) from rat hepatocytes incorporated into lipid bilayers...
  8. van de Loo S, Eich F, Nonis D, Auburger G, Nowock J. Ataxin-2 associates with rough endoplasmic reticulum. Exp Neurol. 2009;215:110-8 pubmed publisher
    ..These data, in conjunction with the presence of Lsm domains within ataxin-2, suggest that ataxin-2 is involved in the processing of mRNA and/or the regulation of translation...
  9. McFarland T, Milstein M, Cala S. Rough endoplasmic reticulum to junctional sarcoplasmic reticulum trafficking of calsequestrin in adult cardiomyocytes. J Mol Cell Cardiol. 2010;49:556-64 pubmed publisher
    Cardiac calsequestrin (CSQ) is synthesized on rough endoplasmic reticulum (ER), but concentrates within the junctional sarcoplasmic reticulum (SR) lumen where it becomes part of the Ca(2+)-release protein complex...
  10. Matsuo S, Nakagawa H, Kiyomiya K, Kurebe M. Fluoride-induced ultrastructural changes in exocrine pancreas cells of rats: fluoride disrupts the export of zymogens from the rough endoplasmic reticulum (rER). Arch Toxicol. 2000;73:611-7 pubmed
    ..Although the rough endoplasmic reticulum (rER) of exocrine pancreas cells in control rats was laminated and oriented parallel to the ..
  11. Loftus T, Nguyen Y, Stanbridge E. The QM protein associates with ribosomes in the rough endoplasmic reticulum. Biochemistry. 1997;36:8224-30 pubmed
    ..Subcellular fractionation demonstrated an association of QM protein with the rough endoplasmic reticulum. It was possible to disrupt this association by washing microsomal membranes with 1M NaCl, suggesting a ..
  12. Abu Kwaik Y. The phagosome containing Legionella pneumophila within the protozoan Hartmannella vermiformis is surrounded by the rough endoplasmic reticulum. Appl Environ Microbiol. 1996;62:2022-8 pubmed
    ..of amoebae is dependent on its capacity to reside and multiply within a phagosome surrounded by the rough endoplasmic reticulum. This compartment may constitute a rich source of nutrients for the bacteria and is probably recognized ..
  13. Merritt T, Bick R, Poindexter B, Alcorn J, Hecht J. Unique matrix structure in the rough endoplasmic reticulum cisternae of pseudoachondroplasia chondrocytes. Am J Pathol. 2007;170:293-300 pubmed
    ..only one mutant COMP subunit may result in an abnormal complex that is accumulated in expanded rough endoplasmic reticulum (rER) cisternae, a hallmark of PSACH...
  14. Ogata T, Yamasaki Y. The tubulovesicular system of gastric parietal cells is connected to the intracellular canaliculus, rough endoplasmic reticulum and Golgi complex. A study by high resolution scanning electron microscopy. Ital J Anat Embryol. 2001;106:323-8 pubmed
    ..Continuity between these two membrane compartments suggests the probability of rapid membrane transposition when cells are stimulated to secrete acid...
  15. Arnold H, Pluta H, Braunbeck T. Sublethal effects of prolonged exposure to disulfoton in rainbow trout (Oncorhynchus mykiss): cytological alterations in the liver by a potent acetylcholine esterase inhibitor. Ecotoxicol Environ Saf. 1996;34:43-55 pubmed
    ..Effects were most evident within cisternae of the rough endoplasmic reticulum (RER), thus suggesting modifications of protein metabolism...
  16. Borradaile N, Han X, Harp J, Gale S, Ory D, Schaffer J. Disruption of endoplasmic reticulum structure and integrity in lipotoxic cell death. J Lipid Res. 2006;47:2726-37 pubmed
  17. Kaiserling E, Muller H. Neoplasm of hepatic stellate cells (spongiotic pericytoma): a new tumor entity in human liver. Pathol Res Pract. 2005;201:733-43 pubmed
    ..The tumor cells possessed extensive cytoplasmic processes, rough endoplasmic reticulum, a prominent Golgi complex, and, in isolated cases, fat droplets...
  18. Lai E, Teodoro T, Volchuk A. Endoplasmic reticulum stress: signaling the unfolded protein response. Physiology (Bethesda). 2007;22:193-201 pubmed
    ..This review will examine UPR signaling that results in cell protective responses, as well as the mechanisms leading to apoptosis induction, which can lead to pathological states due to chronic ER stress...
  19. Torres S, Finol H, Montes de Oca M, Vásquez F, Puigbó J, Loyo J. Capillary damage in skeletal muscle in advanced Chagas' disease patients. Parasitol Res. 2004;93:364-8 pubmed
    ..endothelial cell cytoplasm with dense bodies, large vacuoles, altered mitochondria and prominent rough endoplasmic reticulum, as well as pericyte abnormalities...
  20. King J, Hamil T, Creighton J, Wu S, Bhat P, McDonald F, et al. Structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes. Microvasc Res. 2004;67:139-51 pubmed
    ..Thus, PAECs and PMVECs differ in their structure and function, even under similar environmental conditions...
  21. El Nefiawy N, Abdel Hakim K, Kanayama N, Suganuma N, Terao T. Ontogeny of plasma cells in the early rat yolk sac. Arch Histol Cytol. 2003;66:327-35 pubmed
    ..Immunoelectron microscopy visualized products of the immune reaction in cisterns of the rough endoplasmic reticulum. Conventional electron microscopic examination of 12, 13, and 16-day yolk sacs confirmed the ..
  22. Zhang G, Flick Smith H, McCauley J. Differences in membrane association and sub-cellular distribution between NS2-3 and NS3 of bovine viral diarrhoea virus. Virus Res. 2003;97:89-102 pubmed
    ..This distinct distribution of NS3 and NS2-3 on the ER membrane has revealed a further difference between CP and non-cytopathogenic (NCP) strains of BVDV...
  23. Smith L, Schwarze U, Goldstein J, Byers P. Mutations in the COL3A1 gene result in the Ehlers-Danlos syndrome type IV and alterations in the size and distribution of the major collagen fibrils of the dermis. J Invest Dermatol. 1997;108:241-7 pubmed
    ..In three cases with the mutation G1012R, G1018V, or G1021E, cells in the dermis had extremely dilated rough endoplasmic reticulum (RER), the dermis was thin, and there was a reduced proportion of collagen although the proportion of ..
  24. Iinuma T, Aoki T, Arasaki K, Hirose H, Yamamoto A, Samata R, et al. Role of syntaxin 18 in the organization of endoplasmic reticulum subdomains. J Cell Sci. 2009;122:1680-90 pubmed publisher
    ..These results suggest that syntaxin 18 has an important role in ER subdomain organization by mediating the fusion of retrograde membrane carriers with the ER membrane...
  25. LaFerla F. Calcium dyshomeostasis and intracellular signalling in Alzheimer's disease. Nat Rev Neurosci. 2002;3:862-72 pubmed
  26. Menon A, Watkins WE3rd -, Hrafnsdottir S. Specific proteins are required to translocate phosphatidylcholine bidirectionally across the endoplasmic reticulum. Curr Biol. 2000;10:241-52 pubmed
    ..No biogenic membrane flippases have been identified and there is controversy as to whether proteins are involved at all, whether any membrane protein is sufficient, or whether non-bilayer arrangements of lipids support flip-flop...
  27. Engler C, Kelliher C, Spitze A, Speck C, Eberhart C, Jun A. Unfolded protein response in fuchs endothelial corneal dystrophy: a unifying pathogenic pathway?. Am J Ophthalmol. 2010;149:194-202.e2 pubmed publisher
    ..To assess for activation of the unfolded protein response in corneal endothelium of Fuchs endothelial corneal dystrophy patients...
  28. Moon W, Yu H, Chung M, Kang M, Lee D. Pale bodies in hepatocellular carcinoma. J Korean Med Sci. 2000;15:516-20 pubmed
    ..and contained granular materials of moderate electron density, and were closely related to dilated rough endoplasmic reticulum. These findings support that PBs are secretory fibrinogen accumulated in cystic ER and that such ..
  29. Dekroon R, Armati P. Synthesis and processing of apolipoprotein E in human brain cultures. Glia. 2001;33:298-305 pubmed
    ..apoE in the presence of astrocytes, and that intracellular neuronal apoE is processed through the rough endoplasmic reticulum, golgi, and CD63-positive lysosomes where it may be stored before secretion...
  30. Ikeda H, Ogawa Y, Yoshimoto T. Ultrastructural characteristics of TSH-producing adenomas with special reference to its close similarity to BFA-treated pituitary adenoma cells. Pituitary. 1999;1:221-6 pubmed
    ..Ultrastructural analysis showed the abundance of oval-shaped dilated rough endoplasmic reticulum (rER)...
  31. Jones C, Enders A, Fazleabas A. Early implantation events in the baboon (Papio anubis) with special reference to the establishment of anchoring villi. Placenta. 2001;22:440-56 pubmed
  32. Farah C, Leclerc N. HMWMAP2: new perspectives on a pathway to dendritic identity. Cell Motil Cytoskeleton. 2008;65:515-27 pubmed publisher
  33. Braakman I, Hebert D. Analysis of disulfide bond formation. Curr Protoc Protein Sci. 2001;Chapter 14:Unit14.1 pubmed publisher
    ..The difference in mobility observed between the gels with unreduced and reduced samples is due to disulfide bonds in the unreduced protein...
  34. Schellens J, Vreeling Sindelárová H, Van den Munckhof R, Frederiks W. Electron microscopical study of a cytosolic enzyme in unfixed cryostat sections: demonstration of glycogen phosphorylase activity in rat liver and heart tissue. Histochem J. 1995;27:609-14 pubmed
  35. Dezfuli B, Capuano S, Magosso S, Giari L, Berti R. The lateral line system in larvae of the blind cyprinid cavefish, Phreatichthys andruzzii. Anat Rec (Hoboken). 2009;292:423-30 pubmed publisher
    ..Both types of support cells possessed well-developed rough endoplasmic reticulum and Golgi apparatus...
  36. Tasat D, Orona N, Mandalunis P, Cabrini R, Ubios A. Ultrastructural and metabolic changes in osteoblasts exposed to uranyl nitrate. Arch Toxicol. 2007;81:319-26 pubmed
    ..controls. Our results show that UN modifies osteoblast cell metabolism by increasing ROS generation and reducing APh activity, suggesting that ROS may play a more complex role in cell physiology than simply causing oxidative damage...
  37. Sakai Y, Yamashina S, Furudate S. Missing secretory granules, dilated endoplasmic reticulum, and nuclear dislocation in the thyroid gland of rdw rats with hereditary dwarfism. Anat Rec. 2000;259:60-6 pubmed
    ..substantial quantity inside the dilated rER and in the huge vacuoles that are formed by swelling of the rough endoplasmic reticulum (rER) at the basal side of the follicular epithelial cells...
  38. Schuwerack P, Lewis J. The mode of action of acute and chronic concentrations of waterborne Cd in the digestive gland of the acclimated infested freshwater crab ( Potamonautes warreni). Cell Tissue Res. 2003;312:249-63 pubmed
    ..Cd also caused dissociation of the fibrillar rough endoplasmic reticulum (RER) and an increase in phagocytotic activity in F- and B-cells...
  39. Brodie S, Kitoh H, Lachman R, Nolasco L, Mekikian P, Wilcox W. Platyspondylic lethal skeletal dysplasia, San Diego type, is caused by FGFR3 mutations. Am J Med Genet. 1999;84:476-80 pubmed
    ..The most notable difference between TD and the variants is the presence of large rough endoplasmic reticulum (rER) inclusion bodies within chondrocytes of the variants...
  40. Shibata Y, Voeltz G, Rapoport T. Rough sheets and smooth tubules. Cell. 2006;126:435-9 pubmed
    ..Key proteins may drive the formation of these structural morphologies, which in turn could generate the rough and smooth functional domains of the ER...
  41. Kierdorf U, Stoffels E, Stoffels D, Kierdorf H, Szuwart T, Clemen G. Histological studies of bone formation during pedicle restoration and early antler regeneration in roe deer and fallow deer. Anat Rec A Discov Mol Cell Evol Biol. 2003;273:741-51 pubmed
    ..This parallels the situation in other cases of chondrogenesis in membrane bones...
  42. Tandler B, Phillips C. Ultrastructure of the submandibular gland of the rare white-winged vampire bat, Diaemus youngi. Eur J Morphol. 2002;40:253-6 pubmed
    ..These serous cells are conventional in structure, with an extensive rough endoplasmic reticulum, scattered dictyosomes, and numerous secretory granules...
  43. Quatacker J. Neurosecretory granule formation in ligated axons: additional arguments for a local differentiation from a Golgi apparatus extension. Histochem J. 2001;33:129-33 pubmed
    ..All this occurs in the absence of signs of exocytosis. The present findings corroborate the view that granule formation occurs via local differentiation at an axon ending...
  44. Krijnse Locker J, Parton R, Fuller S, Griffiths G, Dotti C. The organization of the endoplasmic reticulum and the intermediate compartment in cultured rat hippocampal neurons. Mol Biol Cell. 1995;6:1315-32 pubmed
    ..of a spectrum of compartment-specific markers with the goal of defining the location of the rough endoplasmic reticulum (ER), smooth ER, intermediate compartment, and the Golgi complex...
  45. Muto T, Shigeo K, Kanazawa M, Irie K, Yajima T, Kaku T. Ultrastructural study of synovitis induced by trauma to the rat temporomandibular joint (TMJ). J Oral Pathol Med. 2003;32:25-33 pubmed
    ..Electron microscopy was used to examine the histologic effect of trauma on the rat temporomandibular joint synovial membrane...
  46. Aikawa Y, Kuraoka A, Kondo H, Kawabuchi M, Watanabe T. Involvement of PITPnm, a mammalian homologue of Drosophila rdgB, in phosphoinositide synthesis on Golgi membranes. J Biol Chem. 1999;274:20569-77 pubmed
    ..These results suggest that PITPnm plays a role in phosphoinositide synthesis on the Golgi vacuoles and possibly in the PtdIns signaling pathway in mammalian cells...
  47. Jarial M, Wilkins J. Ultrastructure of the external gill epithelium of the axolotl, Ambystoma mexicanum with reference to ionic transport. J Submicrosc Cytol Pathol. 2003;35:445-55 pubmed
    ..granular cells display microvilli at their surface and their cytoplasm contains abundant mitochondria, rough endoplasmic reticulum, Golgi complexes, vesicles and PAS+ secretory granules that are extruded at the surface, which along ..
  48. Giardino L, Savoldi E, Pontieri F, Berutti E. Russell bodies in dental pulp of permanent human teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;98:760-4 pubmed
    ..We sought to characterize the prevalence of Russell bodies in specimens clinically diagnosed as pulpitis...
  49. Shao L, Huang Q, He M, Zeng H, Wan L, Zhu Q. Changes of occludin expression in intestinal mucosa after burn in rats. Burns. 2005;31:838-44 pubmed
    ..We speculate that up-regulation of epithelial occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction...
  50. Oliveira S, Leite Vilamaior P, Corradi L, Góes R, Taboga S. Cellular and extracellular behavior in the gerbil (Meriones unguiculatus) ventral prostate following different types of castration and the consequences of testosterone replacement. Cell Biol Int. 2007;31:235-45 pubmed
  51. Seki T, Shioda S, Izumi S, Arimura A, Koide R. Electron microscopic observation of pituitary adenylate cyclase-activating polypeptide (PACAP)-containing neurons in the rat retina. Peptides. 2000;21:109-13 pubmed
    ..At the ultrastructural level, PACAP-LI was visible in the plasma membranes, rough endoplasmic reticulum, and cytoplasmic matrix in the PACAP-positive neurons in the inner nuclear layer...
  52. Mayhew T. Quantitative description of the spatial arrangement of organelles in a polarised secretory epithelial cell: the salivary gland acinar cell. J Anat. 1999;194 ( Pt 2):279-85 pubmed
    ..It focuses on spatial arrangements of secretion product stores (zymogen granules), rough endoplasmic reticulum (RER) and mitochondria...
  53. Schaletzky J, Rapoport T. Ribosome binding to and dissociation from translocation sites of the endoplasmic reticulum membrane. Mol Biol Cell. 2006;17:3860-9 pubmed
    ..This could explain how RNC-SRP complexes can overcome the competition by nontranslating ribosomes...