cop coated vesicles

Summary

Summary: TRANSPORT VESICLES formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of COP (coat protein complex) proteins, either COPI or COPII. COPI coated vesicles transport backwards from the cisternae of the GOLGI APPARATUS to the rough endoplasmic reticulum (ENDOPLASMIC RETICULUM, ROUGH), while COPII coated vesicles transport forward from the rough endoplasmic reticulum to the Golgi apparatus.

Top Publications

  1. Fath S, Mancias J, Bi X, Goldberg J. Structure and organization of coat proteins in the COPII cage. Cell. 2007;129:1325-36 pubmed
    ..We also propose that the assembly unit has a central hinge-an arrangement of interlocked alpha-solenoids-about which it can bend to adapt to cages of variable curvature...
  2. Mossessova E, Bickford L, Goldberg J. SNARE selectivity of the COPII coat. Cell. 2003;114:483-95 pubmed
    ..The COPII coat seems to be a specific conductor of the fusogenic forms of these SNAREs, suggesting how vesicle fusion specificity may be programmed during budding...
  3. Yang J, Lee S, Gao M, Bourgoin S, Randazzo P, Premont R, et al. ARFGAP1 promotes the formation of COPI vesicles, suggesting function as a component of the coat. J Cell Biol. 2002;159:69-78 pubmed
    ..Collectively, these findings suggest that ARFGAP1 promotes vesicle formation by functioning as a component of the COPI coat...
  4. Runz H, Miura K, Weiss M, Pepperkok R. Sterols regulate ER-export dynamics of secretory cargo protein ts-O45-G. EMBO J. 2006;25:2953-65 pubmed
    ..Consistent with this, membrane turnover of the COPII component Sec23p is delayed in sterol-depleted cells. Altogether, our results demonstrate the importance of sterol levels in COPII mediated ER-export...
  5. Bhattacharyya D, Glick B. Two mammalian Sec16 homologues have nonredundant functions in endoplasmic reticulum (ER) export and transitional ER organization. Mol Biol Cell. 2007;18:839-49 pubmed
    ..Immunoprecipitation data indicate that Sec16L and Sec16S are each present at multiple copies in a heteromeric complex. We infer that mammalian cells have preserved and extended the function of Sec16. ..
  6. Antonny B, Schekman R. ER export: public transportation by the COPII coach. Curr Opin Cell Biol. 2001;13:438-43 pubmed
  7. Gimeno R, Espenshade P, Kaiser C. COPII coat subunit interactions: Sec24p and Sec23p bind to adjacent regions of Sec16p. Mol Biol Cell. 1996;7:1815-23 pubmed
    ..These findings define binding to Sec16p as a new function for Sec24p and support the idea that Sec16p organizes assembly of the COPII coat...
  8. Shaywitz D, Espenshade P, Gimeno R, Kaiser C. COPII subunit interactions in the assembly of the vesicle coat. J Biol Chem. 1997;272:25413-6 pubmed
    ..We propose that Sec16p organizes the assembly of a coat that is stabilized both by the interaction of Sec31p with Sec23p and Sec24p and by the interaction of these three components with Sec16p...
  9. Springer S, Schekman R. Nucleation of COPII vesicular coat complex by endoplasmic reticulum to Golgi vesicle SNAREs. Science. 1998;281:698-700 pubmed
    ..The data suggest that transmembrane proteins can be taken up into COPII vesicles by direct interactions with the coat proteins and may play a structural role in the assembly of the COPII coat complex...

More Information

Publications62

  1. Higashio H, Kimata Y, Kiriyama T, Hirata A, Kohno K. Sfb2p, a yeast protein related to Sec24p, can function as a constituent of COPII coats required for vesicle budding from the endoplasmic reticulum. J Biol Chem. 2000;275:17900-8 pubmed
  2. Fu L, Sztul E. Traffic-independent function of the Sar1p/COPII machinery in proteasomal sorting of the cystic fibrosis transmembrane conductance regulator. J Cell Biol. 2003;160:157-63 pubmed
    ..These findings reveal a new aspect of the degradative mechanism, and suggest functional crosstalk between the secretory and the degradative pathways...
  3. Lederkremer G, Cheng Y, Petre B, Vogan E, Springer S, Schekman R, et al. Structure of the Sec23p/24p and Sec13p/31p complexes of COPII. Proc Natl Acad Sci U S A. 2001;98:10704-9 pubmed
    ..Putting together the architecture of these Sec complexes with the interactions between their subunits and the appearance of the coat in COPII-coated vesicles, we present a model for COPII coat organization...
  4. Watson P, Forster R, Palmer K, Pepperkok R, Stephens D. Coupling of ER exit to microtubules through direct interaction of COPII with dynactin. Nat Cell Biol. 2005;7:48-55 pubmed
    ..Together, our data suggest a mechanism by which membranes of the early secretory pathway can be linked to motors and microtubules for subsequent organization and movement to the Golgi apparatus...
  5. Connerly P, Esaki M, Montegna E, Strongin D, Levi S, Soderholm J, et al. Sec16 is a determinant of transitional ER organization. Curr Biol. 2005;15:1439-47 pubmed
    ..However, little is known about how COPII components are concentrated at tER sites. The budding yeast Pichia pastoris contains discrete tER sites and is, therefore, an ideal system for studying tER organization...
  6. Antonny B, Gounon P, Schekman R, Orci L. Self-assembly of minimal COPII cages. EMBO Rep. 2003;4:419-24 pubmed
    ..We suggest that these structures, which represent a minimal COPII cage, mimic the molecular organization of the membrane-associated COPII coat...
  7. Forster R, Weiss M, Zimmermann T, Reynaud E, Verissimo F, Stephens D, et al. Secretory cargo regulates the turnover of COPII subunits at single ER exit sites. Curr Biol. 2006;16:173-9 pubmed
    ..We conclude that secretory cargo retains the COPII complex on membranes, after Sar1p release has occurred, and prevents premature disassembly of COPII during cargo sorting and transport carrier formation...
  8. Supek F, Madden D, Hamamoto S, Orci L, Schekman R. Sec16p potentiates the action of COPII proteins to bud transport vesicles. J Cell Biol. 2002;158:1029-38 pubmed
    ..We propose that Sec16p nucleates a Sar1-GTP-dependent initiation of COPII assembly and serves to stabilize the coat to premature disassembly after Sar1p hydrolyzes GTP...
  9. Kuehn M, Herrmann J, Schekman R. COPII-cargo interactions direct protein sorting into ER-derived transport vesicles. Nature. 1998;391:187-90 pubmed
    ..Our results indicate that cargo packaging signals and soluble cargo adaptors are recognized by a recruitment complex comprising Sar1-GTP and Sec23/24...
  10. Matsuoka K, Schekman R, Orci L, Heuser J. Surface structure of the COPII-coated vesicle. Proc Natl Acad Sci U S A. 2001;98:13705-9 pubmed
    ..Partially uncoated profiles revealed strands of Sec13/31p stripped from the vesicle surface. We conclude that the coat subunits form layers displaced from the membrane surface in reverse order of addition to the coat...
  11. Mironov A, Beznoussenko G, Trucco A, Lupetti P, Smith J, Geerts W, et al. ER-to-Golgi carriers arise through direct en bloc protrusion and multistage maturation of specialized ER exit domains. Dev Cell. 2003;5:583-94 pubmed
    ..Fully protruded saccules then move centripetally, evolving into one of two types of carriers (with distinct kinetic and structural features). These findings provide an alternative framework for analysis of ER-to-Golgi traffic...
  12. Yoshihisa T, Barlowe C, Schekman R. Requirement for a GTPase-activating protein in vesicle budding from the endoplasmic reticulum. Science. 1993;259:1466-8 pubmed
    ..It appears that Sec23p represents a new class of GTPase-activating protein because its sequence shows no similarity to any known member of this family...
  13. Pelham H, Rothman J. The debate about transport in the Golgi--two sides of the same coin?. Cell. 2000;102:713-9 pubmed
  14. Bentley M, Liang Y, Mullen K, Xu D, Sztul E, Hay J. SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion. J Biol Chem. 2006;281:38825-33 pubmed
    ..We anticipate that recruitment of tether molecules by an upstream SNARE signal ensures that tethering events are initiated only at focal sites containing appropriately poised fusion machinery...
  15. Bi X, Mancias J, Goldberg J. Insights into COPII coat nucleation from the structure of Sec23.Sar1 complexed with the active fragment of Sec31. Dev Cell. 2007;13:635-45 pubmed
    ..Substitution of the corresponding residue F382L in human Sec23A causes cranio-lenticulo-sutural dysplasia, and we suggest that this mutation disrupts the nucleation of COPII coat proteins at endoplasmic reticulum exit sites...
  16. Barlowe C. Traffic COPs of the early secretory pathway. Traffic. 2000;1:371-7 pubmed
    ..This review focuses on recent advances in molecular mechanisms underlying coated-vesicle assembly and connections with cellular structures...
  17. Sacher M, Barrowman J, Wang W, Horecka J, Zhang Y, Pypaert M, et al. TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport. Mol Cell. 2001;7:433-42 pubmed
    ..Our findings imply that TRAPP I is the receptor on the Golgi for COPII vesicles. Once the vesicle binds to TRAPP I, the small GTP binding protein Ypt1p is activated and other tethering factors are recruited...
  18. Shimoni Y, Kurihara T, Ravazzola M, Amherdt M, Orci L, Schekman R. Lst1p and Sec24p cooperate in sorting of the plasma membrane ATPase into COPII vesicles in Saccharomyces cerevisiae. J Cell Biol. 2000;151:973-84 pubmed
    ..By changing the geometry of COPII coat polymerization, Lst1p may allow the transport of bulky cargo molecules, polymers, or particles...
  19. Stagg S, Gurkan C, Fowler D, LaPointe P, Foss T, Potter C, et al. Structure of the Sec13/31 COPII coat cage. Nature. 2006;439:234-8 pubmed
    ..Our data are consistent with a model for COPII coat complex assembly in which Sec23/24 has a non-structural role as a multivalent ligand localizing the self-assembly of Sec13/31 to form a cage lattice driving ER cargo export...
  20. Lee M, Orci L, Hamamoto S, Futai E, Ravazzola M, Schekman R. Sar1p N-terminal helix initiates membrane curvature and completes the fission of a COPII vesicle. Cell. 2005;122:605-17 pubmed
    ..Thus, the initiation of vesicle budding by Sar1p couples the generation of membrane curvature with coat-protein assembly and cargo capture...
  21. Hughes H, Budnik A, Schmidt K, Palmer K, Mantell J, Noakes C, et al. Organisation of human ER-exit sites: requirements for the localisation of Sec16 to transitional ER. J Cell Sci. 2009;122:2924-34 pubmed publisher
    ..The central conserved domain of Sec16 binds to Sec13 linking tER membrane localisation with COPII vesicle formation. These data are consistent with a model where Sec16 acts as a platform for COPII assembly at ERES...
  22. Kim J, Hamamoto S, Ravazzola M, Orci L, Schekman R. Uncoupled packaging of amyloid precursor protein and presenilin 1 into coat protein complex II vesicles. J Biol Chem. 2005;280:7758-68 pubmed
    ..Titration of Sarla distinguished the packaging requirements of APP and PS1. Furthermore, APP packaging was not affected by deletion of PS1 or PS1 and 2, suggesting APP and PS1 trafficking from the ER are normally uncoupled...
  23. Yang Y, Elamawi R, Bubeck J, Pepperkok R, Ritzenthaler C, Robinson D. Dynamics of COPII vesicles and the Golgi apparatus in cultured Nicotiana tabacum BY-2 cells provides evidence for transient association of Golgi stacks with endoplasmic reticulum exit sites. Plant Cell. 2005;17:1513-31 pubmed
    ..By contrast, Golgi stacks associate intermittently and sometimes concurrently with several ERES as they move...
  24. Ward T, Polishchuk R, Caplan S, Hirschberg K, Lippincott Schwartz J. Maintenance of Golgi structure and function depends on the integrity of ER export. J Cell Biol. 2001;155:557-70 pubmed
    ..Instead, they suggest that the Golgi complex is a dynamic, steady-state system, whose membranes can be nucleated and are maintained by the activities of the Sar1-COPII and Arf1-coatomer systems...
  25. Mancias J, Goldberg J. Exiting the endoplasmic reticulum. Traffic. 2005;6:278-85 pubmed
    ..Recent work has highlighted the function of transitional ER regions in specifying the location of COPII budding...
  26. Rabouille C, Jokitalo E. Golgi apparatus partitioning during cell division. Mol Membr Biol. 2003;20:117-27 pubmed
    ..We will also review how the study of the Golgi apparatus during mitosis in other organisms can answer current questions and perhaps reveal novel mechanisms...
  27. Chen C, Calero M, DeRegis C, Heidtman M, Barlowe C, Collins R. Genetic analysis of yeast Yip1p function reveals a requirement for Golgi-localized rab proteins and rab-Guanine nucleotide dissociation inhibitor. Genetics. 2004;168:1827-41 pubmed
  28. Miller E, Liu Y, Barlowe C, Schekman R. ER-Golgi transport defects are associated with mutations in the Sed5p-binding domain of the COPII coat subunit, Sec24p. Mol Biol Cell. 2005;16:3719-26 pubmed
    ..We propose that the A-site of Sec24p is a multipurpose cargo-binding site that must recognize additional unidentified cargo proteins, at least one of which is essential at a late stage of vesicle fusion...
  29. Palmer K, Konkel J, Stephens D. PCTAIRE protein kinases interact directly with the COPII complex and modulate secretory cargo transport. J Cell Sci. 2005;118:3839-47 pubmed
    ..These data show a role for PCTAIRE protein kinase function in membrane traffic through the early secretory pathway...
  30. Hughes H, Stephens D. Assembly, organization, and function of the COPII coat. Histochem Cell Biol. 2008;129:129-51 pubmed
    ..A significant outcome of such a full understanding is to reveal how the machinery and processes of membrane trafficking through the early secretory pathway fail in disease states...
  31. Matsuoka K, Orci L, Amherdt M, Bednarek S, Hamamoto S, Schekman R, et al. COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes. Cell. 1998;93:263-75 pubmed
    ..These observations suggest that the assembly of the COPII coat on the ER occurs by a sequential binding of coat proteins to specific lipids and that this assembly promotes the budding of COPII-coated vesicles...
  32. Prescott A, Farmaki T, Thomson C, James J, Paccaud J, Tang B, et al. Evidence for prebudding arrest of ER export in animal cell mitosis and its role in generating Golgi partitioning intermediates. Traffic. 2001;2:321-35 pubmed
    ..The vesiculotubular Golgi remnants, containing populations of slow or nonrecycling Golgi components, arise by fragmentation of a depleted Golgi ribbon independently from the ER...
  33. Nagaya H, Wada I, Jia Y, Kanoh H. Diacylglycerol kinase delta suppresses ER-to-Golgi traffic via its SAM and PH domains. Mol Biol Cell. 2002;13:302-16 pubmed
    ..These findings indicate that DGKdelta negatively regulates ER-to-Golgi traffic by selectively inhibiting the formation of ER export sites without significantly affecting retrograde transport...
  34. Shorter J, Beard M, Seemann J, Dirac Svejstrup A, Warren G. Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115. J Cell Biol. 2002;157:45-62 pubmed
    ..Staging experiments reveal that the linking of Golgins precedes SNAREpin assembly. Thus, p115 coordinates sequential tethering and docking of COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs)...
  35. Bi X, Corpina R, Goldberg J. Structure of the Sec23/24-Sar1 pre-budding complex of the COPII vesicle coat. Nature. 2002;419:271-7 pubmed
    ..These observations establish the structural basis for GTP-dependent recruitment of a vesicular coat complex, and for uncoating through coat-controlled GTP hydrolysis...
  36. Aridor M, Guzik A, Bielli A, Fish K. Endoplasmic reticulum export site formation and function in dendrites. J Neurosci. 2004;24:3770-6 pubmed
    ..We propose that regulation of receptor assembly and export from the ER in dendrites plays an important role in modulating receptor surface expression and neuronal function...
  37. Eugster A, Frigerio G, Dale M, Duden R. The alpha- and beta'-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. Mol Biol Cell. 2004;15:1011-23 pubmed
  38. Miller E, Beilharz T, Malkus P, Lee M, Hamamoto S, Orci L, et al. Multiple cargo binding sites on the COPII subunit Sec24p ensure capture of diverse membrane proteins into transport vesicles. Cell. 2003;114:497-509 pubmed
    ..Together, our data support a model whereby Sec24p proteins contain multiple independent cargo binding domains that allow for recognition of a diverse set of sorting signals...
  39. Chen J, Lacomis L, Erdjument Bromage H, Tempst P, Stamnes M. Cytosol-derived proteins are sufficient for Arp2/3 recruitment and ARF/coatomer-dependent actin polymerization on Golgi membranes. FEBS Lett. 2004;566:281-6 pubmed
    ..These results show that activated ARF1 can stimulate Arp2/3 recruitment to Golgi membranes through coatomer, Cdc42 or Rac, and N-WASP...
  40. Sun L, Li L, Goldstein J, Brown M. Insig required for sterol-mediated inhibition of Scap/SREBP binding to COPII proteins in vitro. J Biol Chem. 2005;280:26483-90 pubmed publisher
    ..This hexapeptide acts as a sterol-regulated ER sorting signal. These studies define the biochemical parameters responsible for regulated sorting of an ER membrane protein into COPII-coated vesicles...
  41. Espenshade P, Gimeno R, Holzmacher E, Teung P, Kaiser C. Yeast SEC16 gene encodes a multidomain vesicle coat protein that interacts with Sec23p. J Cell Biol. 1995;131:311-24 pubmed
    ..Together, these results suggest that Sec16p engages in multiple protein-protein interactions both on the ER membrane and as part of the coat of a completed vesicle...
  42. Barlowe C. Signals for COPII-dependent export from the ER: what's the ticket out?. Trends Cell Biol. 2003;13:295-300 pubmed
    ..Certain soluble cargo molecules depend on receptor-like proteins for efficient ER export, although signals that direct soluble cargo into ER-derived vesicles are less defined...
  43. Miller E, Antonny B, Hamamoto S, Schekman R. Cargo selection into COPII vesicles is driven by the Sec24p subunit. EMBO J. 2002;21:6105-13 pubmed
    ..Our data suggest that the principle role of Sec24p is to discriminate cargo molecules for incorporation into COPII vesicles...
  44. Gurkan C, Stagg S, LaPointe P, Balch W. The COPII cage: unifying principles of vesicle coat assembly. Nat Rev Mol Cell Biol. 2006;7:727-38 pubmed
    ..These studies are beginning to produce a unifying molecular and structural model of coat function in the formation and fission of vesicles and tubules in endomembrane traffic...
  45. Amodio G, Renna M, Paladino S, Venturi C, Tacchetti C, Moltedo O, et al. Endoplasmic reticulum stress reduces the export from the ER and alters the architecture of post-ER compartments. Int J Biochem Cell Biol. 2009;41:2511-21 pubmed publisher
  46. Aridor M, Fish K, Bannykh S, Weissman J, Roberts T, Lippincott Schwartz J, et al. The Sar1 GTPase coordinates biosynthetic cargo selection with endoplasmic reticulum export site assembly. J Cell Biol. 2001;152:213-29 pubmed
    ..Our results demonstrate an unanticipated and novel role for Sar1 in linking cargo selection with ER morphogenesis through the generation of transitional tubular ER export sites...
  47. Rein U, Andag U, Duden R, Schmitt H, Spang A. ARF-GAP-mediated interaction between the ER-Golgi v-SNAREs and the COPI coat. J Cell Biol. 2002;157:395-404 pubmed
    ..The mechanisms by which v-SNAREs interact with COPI and COPII coat proteins seem to be different and may play a key role in determining specificity in vesicle budding...
  48. Bevis B, Hammond A, Reinke C, Glick B. De novo formation of transitional ER sites and Golgi structures in Pichia pastoris. Nat Cell Biol. 2002;4:750-6 pubmed
    ..Golgi dynamics are similar, although late Golgi elements often move away from tER sites towards regions of polarized growth. Our results can be explained by assuming that tER sites give rise to Golgi cisternae that continually mature...
  49. Nebenf├╝hr A. Vesicle traffic in the endomembrane system: a tale of COPs, Rabs and SNAREs. Curr Opin Plant Biol. 2002;5:507-12 pubmed
    ..Novel tools and a better understanding of the molecular effects of the inhibitor brefeldin A are helping to unravel these plant-specific adaptations...
  50. Reinhard C, Schweikert M, Wieland F, Nickel W. Functional reconstitution of COPI coat assembly and disassembly using chemically defined components. Proc Natl Acad Sci U S A. 2003;100:8253-7 pubmed
    ..Bourgoin, S., Randazzo, P. A., et al. (2002) J. Cell Biol. 159, 69-78]. Thus, a complete round of COPI coat assembly and disassembly has been reconstituted with purified components defining the core machinery of COPI vesicle biogenesis...
  51. Tang B, Wang Y, Ong Y, Hong W. COPII and exit from the endoplasmic reticulum. Biochim Biophys Acta. 2005;1744:293-303 pubmed
    ..Together, elucidation of COPII-mediated ER export has painted a fascinating picture of molecular complexity for an essential process in all eukaryotic cells...
  52. Duden R. ER-to-Golgi transport: COP I and COP II function (Review). Mol Membr Biol. 2003;20:197-207 pubmed
    ..Additionally, COP I coat proteins have complex functions in intra-Golgi trafficking and in maintaining the normal structure of the mammalian interphase Golgi complex...
  53. Bielli A, Haney C, Gabreski G, Watkins S, Bannykh S, Aridor M. Regulation of Sar1 NH2 terminus by GTP binding and hydrolysis promotes membrane deformation to control COPII vesicle fission. J Cell Biol. 2005;171:919-24 pubmed
    ..Thus Sar1-mediated GTP binding and hydrolysis regulates the NH2-terminal tail to perturb membrane packing, promote membrane deformation, and control vesicle fission...