clathrin coated vesicles

Summary

Summary: Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of the protein CLATHRIN. Shortly after formation, however, the clathrin coat is removed and the vesicles are referred to as ENDOSOMES.

Top Publications

  1. Newmyer S, Christensen A, Sever S. Auxilin-dynamin interactions link the uncoating ATPase chaperone machinery with vesicle formation. Dev Cell. 2003;4:929-40 pubmed
    ..The data suggest that dynamin regulates the action of molecular chaperones in vesicle budding during endocytosis...
  2. Ghosh P, Griffith J, Geuze H, Kornfeld S. Mammalian GGAs act together to sort mannose 6-phosphate receptors. J Cell Biol. 2003;163:755-66 pubmed
    ..The morphology of the TGN was also altered. These findings indicate that the three mammalian GGAs cooperate to sort cargo and are required for maintenance of TGN structure...
  3. Doray B, Kornfeld S. Gamma subunit of the AP-1 adaptor complex binds clathrin: implications for cooperative binding in coated vesicle assembly. Mol Biol Cell. 2001;12:1925-35 pubmed
  4. Meyer D, Crottet P, Maco B, Degtyar E, Cassel D, Spiess M. Oligomerization and dissociation of AP-1 adaptors are regulated by cargo signals and by ArfGAP1-induced GTP hydrolysis. Mol Biol Cell. 2005;16:4745-54 pubmed
    ..GTP and ArfGAP1 on the membrane stimulates Arf1 GTPase activity, whereas interaction with cargo induces oligomerization and reduces the rate of GTP hydrolysis, thus contributing to efficient cargo sorting. ..
  5. Akhtar N, Hotchin N. RAC1 regulates adherens junctions through endocytosis of E-cadherin. Mol Biol Cell. 2001;12:847-62 pubmed
    ..Our data suggest that Rac1 regulates adherens junctions via clathrin independent endocytosis of E-cadherin...
  6. Rapoport I, Boll W, Yu A, Böcking T, Kirchhausen T. A motif in the clathrin heavy chain required for the Hsc70/auxilin uncoating reaction. Mol Biol Cell. 2008;19:405-13 pubmed
    ..Thus, the QLMLT motif is required for Hsc-70-facilitated uncoating, consistent with the proposal that this sequence is a specific target of the chaperone...
  7. Smythe E, Ayscough K. Actin regulation in endocytosis. J Cell Sci. 2006;119:4589-98 pubmed
    ..Furthermore, live cell imaging indicates that spatiotemporal aspects of actin recruitment and vesicle formation are likely to be conserved across eukaryotic evolution...
  8. Ford M, Pearse B, Higgins M, Vallis Y, Owen D, Gibson A, et al. Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes. Science. 2001;291:1051-5 pubmed
    ..This was shown by using purified components and a budding assay on preformed lipid monolayers. In the presence of AP180, clathrin lattices formed on the monolayer. When AP2 was also present, coated pits were formed...
  9. Sato M, Sato K, Fonarev P, Huang C, Liou W, Grant B. Caenorhabditis elegans RME-6 is a novel regulator of RAB-5 at the clathrin-coated pit. Nat Cell Biol. 2005;7:559-69 pubmed
    ..These results suggest a mechanism for the activation of Rab5 in clathrin-coated pits or clathrin-coated vesicles that is essential for the delivery of endocytic cargo to early endosomes...

More Information

Publications62

  1. Wasiak S, Legendre Guillemin V, Puertollano R, Blondeau F, Girard M, de Heuvel E, et al. Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics. J Cell Biol. 2002;158:855-62 pubmed
    ..These data suggest a role for enthoprotin in clathrin-mediated budding on internal membranes. Our study reveals the utility of proteomics in the identification of novel vesicle trafficking proteins...
  2. Engqvist Goldstein A, Warren R, Kessels M, Keen J, Heuser J, Drubin D. The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro. J Cell Biol. 2001;154:1209-23 pubmed
    ..In total, Hip1R's properties suggest an early endocytic function at the interface between clathrin, F-actin, and lipids...
  3. Newpher T, Idrissi F, Geli M, Lemmon S. Novel function of clathrin light chain in promoting endocytic vesicle formation. Mol Biol Cell. 2006;17:4343-52 pubmed
    ..We propose that LC binding to Sla2p positively regulates Sla2p for efficient endocytic vesicle formation...
  4. Mishra S, Watkins S, Traub L. The autosomal recessive hypercholesterolemia (ARH) protein interfaces directly with the clathrin-coat machinery. Proc Natl Acad Sci U S A. 2002;99:16099-104 pubmed
  5. Motley A, Bright N, Seaman M, Robinson M. Clathrin-mediated endocytosis in AP-2-depleted cells. J Cell Biol. 2003;162:909-18 pubmed
    ..Uptake of the EGF and LDL receptors may be facilitated by alternative adaptors...
  6. Brodsky F, Chen C, Knuehl C, Towler M, Wakeham D. Biological basket weaving: formation and function of clathrin-coated vesicles. Annu Rev Cell Dev Biol. 2001;17:517-68 pubmed
    ..Tissue-specific functions of CCVs require even more-specialized regulation and defects in these pathways can now be correlated with human diseases...
  7. Morgan J, Prasad K, Jin S, Augustine G, Lafer E. Uncoating of clathrin-coated vesicles in presynaptic terminals: roles for Hsc70 and auxilin. Neuron. 2001;32:289-300 pubmed
    ..These studies establish that auxilin and Hsc70 participate in synaptic vesicle recycling in neurons and that an interaction between these proteins is required for CCV uncoating...
  8. Di Guglielmo G, Le Roy C, Goodfellow A, Wrana J. Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover. Nat Cell Biol. 2003;5:410-21 pubmed
    ..Thus, segregation of TGF-beta receptors into distinct endocytic compartments regulates Smad activation and receptor turnover...
  9. Veiga E, Cossart P. Listeria hijacks the clathrin-dependent endocytic machinery to invade mammalian cells. Nat Cell Biol. 2005;7:894-900 pubmed
  10. Borner G, Harbour M, Hester S, Lilley K, Robinson M. Comparative proteomics of clathrin-coated vesicles. J Cell Biol. 2006;175:571-8 pubmed
    ..The strategy outlined in this paper should be widely applicable as a means of distinguishing genuine organelle components from contaminants...
  11. Takei K, Haucke V. Clathrin-mediated endocytosis: membrane factors pull the trigger. Trends Cell Biol. 2001;11:385-91 pubmed
    ..In this review, we focus on the role of membrane proteins and lipids in the nucleation of clathrin-coated pits and provide a hypothetical model for the early steps in clathrin-mediated endocytosis...
  12. Girard M, Allaire P, McPherson P, Blondeau F. Non-stoichiometric relationship between clathrin heavy and light chains revealed by quantitative comparative proteomics of clathrin-coated vesicles from brain and liver. Mol Cell Proteomics. 2005;4:1145-54 pubmed
  13. Heymann J, Iwasaki K, Yim Y, Cheng N, Belnap D, Greene L, et al. Visualization of the binding of Hsc70 ATPase to clathrin baskets: implications for an uncoating mechanism. J Biol Chem. 2005;280:7156-61 pubmed
    ..Three Hsc70s remain bound to the dissociated triskelion, close to its trimerization hub...
  14. Yarar D, Waterman Storer C, Schmid S. A dynamic actin cytoskeleton functions at multiple stages of clathrin-mediated endocytosis. Mol Biol Cell. 2005;16:964-75 pubmed
    ..Our results demonstrate a key role for actin during clathrin-mediated endocytosis in mammalian cells...
  15. Newmyer S, Schmid S. Dominant-interfering Hsc70 mutants disrupt multiple stages of the clathrin-coated vesicle cycle in vivo. J Cell Biol. 2001;152:607-20 pubmed
    ..Our findings demonstrate that hsc70 indeed regulates coat disassembly and also suggest that this chaperone broadly modulates clathrin dynamics throughout the CCV cycle...
  16. Merrifield C, Perrais D, Zenisek D. Coupling between clathrin-coated-pit invagination, cortactin recruitment, and membrane scission observed in live cells. Cell. 2005;121:593-606 pubmed publisher
    ..We propose that CCP invagination, actin polymerization, and CCV formation are highly coordinated for efficient endocytosis...
  17. Cheng Y, Boll W, Kirchhausen T, Harrison S, Walz T. Cryo-electron tomography of clathrin-coated vesicles: structural implications for coat assembly. J Mol Biol. 2007;365:892-9 pubmed
    ..We suggest that the eccentric position of the vesicle reflects the polarity of assembly, from initiation of coat formation to membrane pinching...
  18. Rust M, Lakadamyali M, Zhang F, Zhuang X. Assembly of endocytic machinery around individual influenza viruses during viral entry. Nat Struct Mol Biol. 2004;11:567-73 pubmed
    ..CCP formation at these sites is much faster than elsewhere on the cell surface, suggesting a virus-induced CCP formation mechanism that may be commonly exploited by many other types of viruses...
  19. Schmid E, Ford M, Burtey A, Praefcke G, Peak Chew S, Mills I, et al. Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly. PLoS Biol. 2006;4:e262 pubmed
    ..However, proteins such as beta-arrestin (non-visual arrestin) and autosomal recessive hypercholesterolemia protein, which have direct clathrin interactions, will remain in the coated pits with their interacting receptors...
  20. Jiang X, Huang F, Marusyk A, Sorkin A. Grb2 regulates internalization of EGF receptors through clathrin-coated pits. Mol Biol Cell. 2003;14:858-70 pubmed
    ..The data are consistent with the model whereby Grb2 participates in EGFR internalization through the recruitment of Cbl to the receptor, thus allowing proper ubiquitylation of EGFR and/or associated proteins at the plasma membrane...
  21. Fotin A, Kirchhausen T, Grigorieff N, Harrison S, Walz T, Cheng Y. Structure determination of clathrin coats to subnanometer resolution by single particle cryo-electron microscopy. J Struct Biol. 2006;156:453-60 pubmed
    ..These procedures should be generally applicable and may thus help determine the structures of other large protein assemblies to higher resolution by single particle cryo-electron microscopy...
  22. Howe C, Valletta J, Rusnak A, Mobley W. NGF signaling from clathrin-coated vesicles: evidence that signaling endosomes serve as a platform for the Ras-MAPK pathway. Neuron. 2001;32:801-14 pubmed
    ..Our results point to the existence of a population of signaling endosomes derived from clathrin-coated membranes in NGF-treated cells...
  23. Buss F, Luzio J, Kendrick Jones J. Myosin VI, a new force in clathrin mediated endocytosis. FEBS Lett. 2001;508:295-9 pubmed
    ..The possible role(s) of myosin VI in the sequential steps involved in receptor mediated endocytosis are discussed...
  24. Wu X, Zhao X, Baylor L, Kaushal S, Eisenberg E, Greene L. Clathrin exchange during clathrin-mediated endocytosis. J Cell Biol. 2001;155:291-300 pubmed
  25. Scott M, Benmerah A, Muntaner O, Marullo S. Recruitment of activated G protein-coupled receptors to pre-existing clathrin-coated pits in living cells. J Biol Chem. 2002;277:3552-9 pubmed
  26. Tooze S, Martens G, Huttner W. Secretory granule biogenesis: rafting to the SNARE. Trends Cell Biol. 2001;11:116-22 pubmed
    ..At the heart of these findings is the elucidation of molecular mechanisms driving protein--lipid and protein--protein interactions specific for secretory granule biogenesis...
  27. Zoncu R, Perera R, Sebastian R, Nakatsu F, Chen H, Balla T, et al. Loss of endocytic clathrin-coated pits upon acute depletion of phosphatidylinositol 4,5-bisphosphate. Proc Natl Acad Sci U S A. 2007;104:3793-8 pubmed
    ..These findings demonstrate the critical importance of PI(4,5)P(2) in clathrin coat dynamics and Arp2/3-dependent actin regulation...
  28. Deloche O, Yeung B, Payne G, Schekman R. Vps10p transport from the trans-Golgi network to the endosome is mediated by clathrin-coated vesicles. Mol Biol Cell. 2001;12:475-85 pubmed
    ..The Vps10p C-terminal domain appears to play a principal role in retrieval of Vps10p from the prevacuolar compartment rather than in sorting from the TGN...
  29. Greener T, Grant B, Zhang Y, Wu X, Greene L, Hirsh D, et al. Caenorhabditis elegans auxilin: a J-domain protein essential for clathrin-mediated endocytosis in vivo. Nat Cell Biol. 2001;3:215-9 pubmed
    ..We conclude that auxilin is required for in vivo clathrin-mediated endocytosis and development in C. elegans...
  30. Umeda A, Meyerholz A, Ungewickell E. Identification of the universal cofactor (auxilin 2) in clathrin coat dissociation. Eur J Cell Biol. 2000;79:336-42 pubmed
    ..The cellular distribution of membrane-associated adaptors was unaffected by overexpression of GAK. Our results point to a hsc70/auxilin-based uncoating system as a ubiquitous feature of eukaryotic cells...
  31. Drake M, Zhu Y, Kornfeld S. The assembly of AP-3 adaptor complex-containing clathrin-coated vesicles on synthetic liposomes. Mol Biol Cell. 2000;11:3723-36 pubmed
    ..These results establish that AP-3-containing clathrin-coated vesicles form in vitro and are consistent with AP-3-dependent protein transport being mediated by clathrin-coated vesicles...
  32. Hirst J, Sahlender D, Choma M, Sinka R, Harbour M, Parkinson M, et al. Spatial and functional relationship of GGAs and AP-1 in Drosophila and HeLa cells. Traffic. 2009;10:1696-710 pubmed
    ..Thus, our results indicate that the two adaptors can function independently of each other...
  33. Edeling M, Smith C, Owen D. Life of a clathrin coat: insights from clathrin and AP structures. Nat Rev Mol Cell Biol. 2006;7:32-44 pubmed
  34. Kaksonen M, Toret C, Drubin D. A modular design for the clathrin- and actin-mediated endocytosis machinery. Cell. 2005;123:305-20 pubmed
  35. Eisenberg E, Greene L. Multiple roles of auxilin and hsc70 in clathrin-mediated endocytosis. Traffic. 2007;8:640-6 pubmed
  36. Ehrlich M, Boll W, Van Oijen A, Hariharan R, Chandran K, Nibert M, et al. Endocytosis by random initiation and stabilization of clathrin-coated pits. Cell. 2004;118:591-605 pubmed
    ..Cargo incorporation occurs primarily or exclusively in a newly formed coated pit. Our data lead to a model in which coated pits initiate randomly but collapse unless stabilized, perhaps by cargo capture...
  37. Nichols B. Caveosomes and endocytosis of lipid rafts. J Cell Sci. 2003;116:4707-14 pubmed
    ..It is likely that there are mechanisms that allow recruitment and targeting of specific molecules to caveosomes...
  38. Pelkmans L, Helenius A. Insider information: what viruses tell us about endocytosis. Curr Opin Cell Biol. 2003;15:414-22 pubmed
    ..Some of these are subject to regulation, involve novel endocytic organelles, and some of them connect organelles that were previously not known to communicate by membrane traffic...
  39. Raposo G, Tenza D, Murphy D, Berson J, Marks M. Distinct protein sorting and localization to premelanosomes, melanosomes, and lysosomes in pigmented melanocytic cells. J Cell Biol. 2001;152:809-24 pubmed
    ..Furthermore, they implicate an unusual clathrin-coated endosomal compartment as a site from which proteins destined for premelanosomes and lysosomes are sorted...
  40. Crottet P, Meyer D, Rohrer J, Spiess M. ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes. Mol Biol Cell. 2002;13:3672-82 pubmed
    ..The results thus indicate that cargo proteins are involved in coat recruitment and that the local lipid composition contributes to specifying the site of vesicle formation...
  41. Laporte S, Miller W, Kim K, Caron M. beta-Arrestin/AP-2 interaction in G protein-coupled receptor internalization: identification of a beta-arrestin binging site in beta 2-adaptin. J Biol Chem. 2002;277:9247-54 pubmed
    ..These results suggest that the interaction of beta-arrestin with beta(2)-adaptin represents a selective endocytic trigger for several members of the GPCR family...
  42. Peden A, Park G, Scheller R. The Di-leucine motif of vesicle-associated membrane protein 4 is required for its localization and AP-1 binding. J Biol Chem. 2001;276:49183-7 pubmed
    ..Mutation of the di-leucine motif inhibits AP-1 binding in vitro and affects the steady state distribution of VAMP4 in vivo...
  43. Pishvaee B, Costaguta G, Yeung B, Ryazantsev S, Greener T, Greene L, et al. A yeast DNA J protein required for uncoating of clathrin-coated vesicles in vivo. Nat Cell Biol. 2000;2:958-63 pubmed
    ..Our results demonstrate an in vivo uncoating function of a J domain co-chaperone and establish the physiological significance of uncoating in transport mediated by clathrin-coated vesicles...
  44. Zoncu R, Perera R, Balkin D, Pirruccello M, Toomre D, De Camilli P. A phosphoinositide switch controls the maturation and signaling properties of APPL endosomes. Cell. 2009;136:1110-21 pubmed publisher
    ..These findings reveal a surprising plasticity of the early endocytic pathway. Importantly, PI3P functions as a switch to dynamically regulate maturation and signaling of APPL endosomes...
  45. Chen C, Zhuang X. Epsin 1 is a cargo-specific adaptor for the clathrin-mediated endocytosis of the influenza virus. Proc Natl Acad Sci U S A. 2008;105:11790-5 pubmed publisher
    ..These results suggest that epsin 1 functions as a cargo-specific adaptor for the clathrin-mediated entry of the influenza virus...
  46. Kreuzer O, Krisch B, Dery O, Bunnett N, Meyerhof W. Agonist-mediated endocytosis of rat somatostatin receptor subtype 3 involves beta-arrestin and clathrin coated vesicles. J Neuroendocrinol. 2001;13:279-87 pubmed
    ..Thus, agonist-induced endocytosis of sst3 depends on its interaction with beta-arrestin, involves the adaptor protein complex 2 and proceeds via clathrin coated vesicles to the recycling compartment.
  47. Cremona O, De Camilli P. Phosphoinositides in membrane traffic at the synapse. J Cell Sci. 2001;114:1041-52 pubmed
    ..Thus, the reversible phosphorylation of inositol phospholipids may be one of the mechanisms governing the timing and vectorial progression of synaptic vesicle membranes during their exocytic-endocytic cycle...
  48. Ghosh P, Kornfeld S. AP-1 binding to sorting signals and release from clathrin-coated vesicles is regulated by phosphorylation. J Cell Biol. 2003;160:699-708 pubmed
    ..A model is proposed where cyclical phosphorylation/dephosphorylation of the subunits of AP-1 regulate its function from membrane recruitment until its release into cytosol...
  49. Miwako I, Schröter T, Schmid S. Clathrin- and dynamin-dependent coated vesicle formation from isolated plasma membranes. Traffic. 2003;4:376-89 pubmed
    ..This assay will provide a powerful tool to dissect the minimum machinery and to probe the hierarchy of events involved in cargo selection and endocytic clathrin-coated vesicle formation...
  50. Massol R, Boll W, Griffin A, Kirchhausen T. A burst of auxilin recruitment determines the onset of clathrin-coated vesicle uncoating. Proc Natl Acad Sci U S A. 2006;103:10265-10270 pubmed publisher
  51. Traub L. Sorting it out: AP-2 and alternate clathrin adaptors in endocytic cargo selection. J Cell Biol. 2003;163:203-8 pubmed
    ..Remarkably, AP-2 is not obligatory for all clathrin-mediated uptake, and several alternate adaptors appear to perform similar sorting and assembly functions at the clathrin bud site...
  52. Jackson A, Flett A, Smythe C, Hufton L, Wettey F, Smythe E. Clathrin promotes incorporation of cargo into coated pits by activation of the AP2 adaptor micro2 kinase. J Cell Biol. 2003;163:231-6 pubmed
    ..Our results imply a central role for clathrin in the regulation of cargo selection via the modulation of phospho-mu2 levels...
  53. Blondeau F, Ritter B, Allaire P, Wasiak S, Girard M, Hussain N, et al. Tandem MS analysis of brain clathrin-coated vesicles reveals their critical involvement in synaptic vesicle recycling. Proc Natl Acad Sci U S A. 2004;101:3833-8 pubmed
    ..The uncovering of eight previously undescribed proteins, four of which have to date been linked to clathrin-mediated trafficking, further attests to the value of the current organelle-based proteomics strategy...