nuclear matrix

Summary

Summary: The residual framework structure of the CELL NUCLEUS that maintains many of the overall architectural features of the cell nucleus including the nuclear lamina with NUCLEAR PORE complex structures, residual CELL NUCLEOLI and an extensive fibrogranular structure in the nuclear interior. (Advan. Enzyme Regul. 2002; 42:39-52)

Top Publications

  1. Hancock R. Internal organisation of the nucleus: assembly of compartments by macromolecular crowding and the nuclear matrix model. Biol Cell. 2004;96:595-601 pubmed
    ..nucleus are segregated into discrete compartments, but the current model that this is achieved by a fibrillar nuclear matrix which structures the nuclear interior and compartments is not consistent with all experimental observations, ..
  2. Calikowski T, Meulia T, Meier I. A proteomic study of the arabidopsis nuclear matrix. J Cell Biochem. 2003;90:361-78 pubmed
    ..to be organized by two interdependent nucleoprotein structures, the DNA-based chromatin and the RNA-dependent nuclear matrix. The functional composition and molecular organization of the second component have not yet been resolved...
  3. Yeh T, Chuang J, Sung C. Dynein light chain rp3 acts as a nuclear matrix-associated transcriptional modulator in a dynein-independent pathway. J Cell Sci. 2005;118:3431-43 pubmed
    ..Nuclear rp3 binds to and assembles with the transcription factor SATB1 at nuclear matrix-associated structures...
  4. Girard Reydet C, Grégoire D, Vassetzky Y, Mechali M. DNA replication initiates at domains overlapping with nuclear matrix attachment regions in the xenopus and mouse c-myc promoter. Gene. 2004;332:129-38 pubmed
    ..We also identified a nuclear matrix attachment region in both Xenopus and mouse, which is localized to two different regions of the c-myc promoter ..
  5. Drennan K, Linnemann A, Platts A, Heng H, Armant D, Krawetz S. Nuclear matrix association: switching to the invasive cytotrophoblast. Placenta. 2010;31:365-72 pubmed publisher
    ..well characterized invasive and non-invasive behavior, and to correlate the activity of the transcriptome with nuclear matrix attachment and cell phenotype. Comparison of the invasive to non-invasive HTR transcriptomes was unremarkable...
  6. Senderek J, Garvey S, Krieger M, Guergueltcheva V, Urtizberea A, Roos A, et al. Autosomal-dominant distal myopathy associated with a recurrent missense mutation in the gene encoding the nuclear matrix protein, matrin 3. Am J Hum Genet. 2009;84:511-8 pubmed publisher
    ..MATR3 is expressed in skeletal muscle and encodes matrin 3, a component of the nuclear matrix, which is a proteinaceous network that extends throughout the nucleus...
  7. Leman E, Madigan M, Brunagel G, Takaha N, Coffey D, Getzenberg R. Nuclear matrix localization of high mobility group protein I(Y) in a transgenic mouse model for prostate cancer. J Cell Biochem. 2003;88:599-608 pubmed
    Nuclear shape and the underlying nuclear structure, the nuclear matrix in cancer cells. Since the NM composition is considered to maintain nuclear shape and architecture, nuclear matrix proteins (NMPs) may be involved in transformation...
  8. Lee J, Skalnik D. CpG-binding protein is a nuclear matrix- and euchromatin-associated protein localized to nuclear speckles containing human trithorax. Identification of nuclear matrix targeting signals. J Biol Chem. 2002;277:42259-67 pubmed
    ..CGBP associates with the nuclear matrix, and fragments of CGBP that fail to associate with the nuclear matrix fail to localize to nuclear speckles and ..
  9. Iarovaia O, Akopov S, Nikolaev L, Sverdlov E, Razin S. Induction of transcription within chromosomal DNA loops flanked by MAR elements causes an association of loop DNA with the nuclear matrix. Nucleic Acids Res. 2005;33:4157-63 pubmed
    ..It is likely that the loop observed was attached to the nuclear matrix via MAR elements present at the flanks of the area under study...

More Information

Publications62

  1. Dunn K, Zhao H, Davie J. The insulator binding protein CTCF associates with the nuclear matrix. Exp Cell Res. 2003;288:218-23 pubmed
    ..At the base of these loops, matrix-associated regions (MARs) of the DNA interact with nuclear matrix proteins...
  2. Tsutsui K, Sano K, Tsutsui K. Dynamic view of the nuclear matrix. Acta Med Okayama. 2005;59:113-20 pubmed
    The nuclear matrix is an operationally defined nuclear skeletal structure that is believed to be involved in many nuclear functions including DNA replication, transcription, repair, and prem RNA processing/transport...
  3. Simon D, Wilson K. The nucleoskeleton as a genome-associated dynamic 'network of networks'. Nat Rev Mol Cell Biol. 2011;12:695-708 pubmed publisher
    ..These elements constitute a poorly understood 'network of networks' that dynamically reorganizes during mitosis and is responsible for genome organization and integrity...
  4. Guerrero Santoro J, Yang L, Stallcup M, DeFranco D. Distinct LIM domains of Hic-5/ARA55 are required for nuclear matrix targeting and glucocorticoid receptor binding and coactivation. J Cell Biochem. 2004;92:810-9 pubmed
    ..LIM4 also functions as a nuclear matrix targeting sequence (NMTS) for Hic-5/ARA55, as it is both necessary and sufficient to target a heterologous ..
  5. Patterson K, Molofsky A, Robinson C, Acosta S, Cater C, Fischer J. The functions of Klarsicht and nuclear lamin in developmentally regulated nuclear migrations of photoreceptor cells in the Drosophila eye. Mol Biol Cell. 2004;15:600-10 pubmed
    ..The Klarsicht/lamin interaction provides a framework for understanding the mechanistic basis of human laminopathies...
  6. Maya Mendoza A, Hernández Muñoz R, Gariglio P, Aranda Anzaldo A. Gene positional changes relative to the nuclear substructure correlate with the proliferating status of hepatocytes during liver regeneration. Nucleic Acids Res. 2003;31:6168-79 pubmed
    ..is organised in loops anchored to a proteinaceous substructure variously named but commonly known as the nuclear matrix. Important processes of nuclear physiology, such as replication, transcription and processing of primary ..
  7. Bohnsack M, Stüven T, Kuhn C, Cordes V, Gorlich D. A selective block of nuclear actin export stabilizes the giant nuclei of Xenopus oocytes. Nat Cell Biol. 2006;8:257-63 pubmed
    ..Apparently, their mechanical integrity cannot be maintained by chromatin and the associated nuclear matrix, but instead requires an intranuclear actin-scaffold.
  8. Rose A, Gindullis F, Meier I. A novel alpha-helical protein, specific to and highly conserved in plants, is associated with the nuclear matrix fraction. J Exp Bot. 2003;54:1133-41 pubmed
    A cDNA for a novel plant protein was isolated from tomato. Nuclear Matrix Protein 1 (NMP1) is a ubiquitously expressed 36 kDa protein, which has no homologues in animals and fungi, but is highly conserved among flowering and non-flowering ..
  9. He S, Dunn K, Espino P, Drobic B, Li L, Yu J, et al. Chromatin organization and nuclear microenvironments in cancer cells. J Cell Biochem. 2008;104:2004-15 pubmed
    ..b>Nuclear matrix proteins, which include lamins, transcription factors (Sp1) and histone modifying enzymes (histone ..
  10. Wang H, Yu S, Davis A, Ahmed K. Cell cycle dependent regulation of protein kinase CK2 signaling to the nuclear matrix. J Cell Biochem. 2003;88:812-22 pubmed
    ..b>Nuclear matrix is a key locus for CK2 signaling in the nucleus...
  11. Banerjee S, Lohia A. Molecular analysis of repetitive DNA elements from Entamoeba histolytica, which encode small RNAs and contain matrix/scaffold attachment recognition sequences. Mol Biochem Parasitol. 2003;126:35-42 pubmed
    ..Both these sequences bind to high salt extractable nuclear proteins and insoluble nuclear matrix proteins in E...
  12. Evans K, Ott S, Hansen A, Koentges G, Wernisch L. A comparative study of S/MAR prediction tools. BMC Bioinformatics. 2007;8:71 pubmed
    S/MARs are regions of the DNA that are attached to the nuclear matrix. These regions are known to affect substantially the expression of genes...
  13. Tramontano F, Di Meglio S, Quesada P. Co-localization of poly(ADPR)polymerase 1 (PARP-1) poly(ADPR)polymerase 2 (PARP-2) and related proteins in rat testis nuclear matrix defined by chemical cross-linking. J Cell Biochem. 2005;94:58-66 pubmed
    ..Moreover, the association of pADPR and PARP-1 with the nuclear matrix (NM) has been reported, based on the poly(ADP-ribosyl)ation of nuclear matrix proteins (NMPs)...
  14. Kawai T, Nakaya T, Suzuki T. Roles of the intramolecular regions of FE65 in its trans-accumulation and in p53 stabilization in the nuclear matrix of osmotically stressed cells. FEBS Lett. 2010;584:765-9 pubmed publisher
    ..stressed cells, the membrane APP-tethered FE65 is released into the cytoplasm and translocates to the nuclear matrix, where it stabilizes p53 via a non-canonical pathway...
  15. Ioudinkova E, Razin S, Borunova V, De Conto F, Rynditch A, Scherrer K. RNA-dependent nuclear matrix contains a 33 kb globin full domain transcript as well as prosomes but no 26S proteasomes. J Cell Biochem. 2005;94:529-39 pubmed
    Previously, we have shown that in murine myoblasts prosomes are constituents of the nuclear matrix; a major part of the latter was found to be RNase sensitive...
  16. Widłak W, Widłak P. MAR/SAR elements flank the rat hst70 gene transcription unit. Cell Mol Biol Lett. 2004;9:123-33 pubmed
    ..that they possess structural motifs characteristic of MAR/SAR elements, and exhibit enhanced affinities for nuclear matrix binding in vitro. Such elements bind efficiently to matrices from either the testis or the liver, i.e...
  17. Lenartowski R, Goc A. Tissue-specific association of the human tyrosine hydroxylase gene with the nuclear matrix. Neurosci Lett. 2002;330:151-4 pubmed
    Association of the human tyrosine hydroxylase (TH) gene with the nuclear matrix was studied using bovine matrices from tissues differing in expression of the gene...
  18. Donev R, Horton R, Beck S, Doneva T, Vatcheva R, Bowen W, et al. Recruitment of heterogeneous nuclear ribonucleoprotein A1 in vivo to the LMP/TAP region of the major histocompatibility complex. J Biol Chem. 2003;278:5214-26 pubmed
    ..These sequences were shown to function as nuclear matrix attachment regions (MARs)...
  19. Jackson D. The principles of nuclear structure. Chromosome Res. 2003;11:387-401 pubmed
    ..This review sets out to evaluate our present views of the principles that dictate nuclear structure. Particular emphasis is placed on architectural themes and the concept of spatial epigenetics...
  20. Ainscough J, Rahman F, Sercombe H, Sedo A, Gerlach B, Coverley D. C-terminal domains deliver the DNA replication factor Ciz1 to the nuclear matrix. J Cell Sci. 2007;120:115-24 pubmed
    ..that Ciz1 is normally immobilized by interaction with non-chromatin nuclear structures, consistent with the nuclear matrix. Furthermore, matrix-associated Ciz1 foci strikingly colocalize with sites of newly synthesized DNA in S phase ..
  21. Munkley J, Copeland N, Moignard V, Knight J, Greaves E, Ramsbottom S, et al. Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells. Nucleic Acids Res. 2011;39:2671-7 pubmed publisher
    ..We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells...
  22. Dent M, Segura Anaya E, Alva Medina J, Aranda Anzaldo A. NeuN/Fox-3 is an intrinsic component of the neuronal nuclear matrix. FEBS Lett. 2010;584:2767-71 pubmed publisher
    ..Here we provide evidence that NeuN/Fox-3 is an intrinsic component of the neuronal nuclear matrix and a reliable marker of nuclear speckles in neurons.
  23. Martelli A, Falcieri E, Zweyer M, Bortul R, Tabellini G, Cappellini A, et al. The controversial nuclear matrix: a balanced point of view. Histol Histopathol. 2002;17:1193-205 pubmed publisher
    The nuclear matrix is defined as the residual framework after the removal of the nuclear envelope, chromatin, and soluble components by sequential extractions...
  24. Ohsaki E, Suzuki T, Karayama M, Ueda K. Accumulation of LANA at nuclear matrix fraction is important for Kaposi's sarcoma-associated herpesvirus replication in latency. Virus Res. 2009;139:74-84 pubmed publisher
    ..the KSHV genome, and a viral replication factor, latency-associated nuclear antigen (LANA) accumulate at the nuclear matrix fraction in the G1 phase...
  25. Shaman J, Yamauchi Y, Ward W. Function of the sperm nuclear matrix. Arch Androl. 2007;53:135-40 pubmed
    ..One of these is the organization of DNA into loop domains attached at their bases to a proteinaceous nuclear matrix. Several groups have shown that the sites at which DNA associates with the sperm nuclear matrix contain ..
  26. Bala S, Kumar A, Soni S, Sinha S, Hanspal M. Emp is a component of the nuclear matrix of mammalian cells and undergoes dynamic rearrangements during cell division. Biochem Biophys Res Commun. 2006;342:1040-8 pubmed
    ..Sequential extractions of interphase nuclei showed that recombinant Emp was present predominantly in the nuclear matrix. Immunofluorescence microscopy showed that Emp was present in typical nuclear speckles enriched with the ..
  27. Borgatti P, Martelli A, Tabellini G, Bellacosa A, Capitani S, Neri L. Threonine 308 phosphorylated form of Akt translocates to the nucleus of PC12 cells under nerve growth factor stimulation and associates with the nuclear matrix protein nucleolin. J Cell Physiol. 2003;196:79-88 pubmed
    ..Both total and active Akt associated with the nuclear matrix and, in particular, with the protein nucleolin, with which Akt co-immunoprecipitated...
  28. Ostermeier G, Liu Z, Martins R, Bharadwaj R, Ellis J, Draghici S, et al. Nuclear matrix association of the human beta-globin locus utilizing a novel approach to quantitative real-time PCR. Nucleic Acids Res. 2003;31:3257-66 pubmed
    ..b>Nuclear matrix-associated regions (MARs) organize chromatin into functional domains and at least one of the HS appears bound ..
  29. Nalepa G, Harper J. Visualization of a highly organized intranuclear network of filaments in living mammalian cells. Cell Motil Cytoskeleton. 2004;59:94-108 pubmed
    For 30 years, the mammalian cell nucleus has been hypothesized to contain a filamentous framework, the nuclear matrix or karyoskeleton, which regulates nuclear structure and function...
  30. Rivera Mulia J, Hernández Muñoz R, Martinez F, Aranda Anzaldo A. DNA moves sequentially towards the nuclear matrix during DNA replication in vivo. BMC Cell Biol. 2011;12:3 pubmed publisher
    In the interphase nucleus of metazoan cells DNA is organized in supercoiled loops anchored to a nuclear matrix (NM)...
  31. Kantidze O, Iarovaia O, Razin S. Assembly of nuclear matrix-bound protein complexes involved in non-homologous end joining is induced by inhibition of DNA topoisomerase II. J Cell Physiol. 2006;207:660-7 pubmed
    ..Thus, inhibition of topoisomerase II activity triggers assembly of NHEJ complexes at the nuclear matrix.
  32. Eivazova E, Vassetzky Y, Aune T. Selective matrix attachment regions in T helper cell subsets support loop conformation in the Ifng gene. Genes Immun. 2007;8:35-43 pubmed
    ..Here, we analyzed nuclear matrix attachment regions (MARs) in the Ifng gene by DNA array technique in unactivated and activated CD4+ Th cells...
  33. Stein G, Lian J, Van Wijnen A, Stein J, Javed A, Montecino M, et al. Nuclear microenvironments support assembly and organization of the transcriptional regulatory machinery for cell proliferation and differentiation. J Cell Biochem. 2004;91:287-302 pubmed
  34. Bogojevic D, Mihailovic M, Petrovic M, Dinic S, Ivanovic Matic S, Poznanovic G. Acute-phase-dependent binding affinity of C/EBPbeta from the nuclear extract and nuclear matrix towards the hormone response element of the alpha2-macroglobulin gene in rat hepatocytes. Gen Physiol Biophys. 2003;22:279-85 pubmed
    Interactions of nuclear extract and nuclear matrix proteins from rat hepatocytes with the hormone response element of the alpha2-macroglobulin gene were studied...
  35. Pathak R, Rangaraj N, Kallappagoudar S, Mishra K, Mishra R. Boundary element-associated factor 32B connects chromatin domains to the nuclear matrix. Mol Cell Biol. 2007;27:4796-806 pubmed
    ..In this report we show that BEAF is associated with the nuclear matrix and map the domain required for matrix association to the middle region of the protein...
  36. Barboro P, D Arrigo C, Repaci E, Patrone E, Balbi C. Organization of the lamin scaffold in the internal nuclear matrix of normal and transformed hepatocytes. Exp Cell Res. 2010;316:992-1001 pubmed publisher
    Nuclear lamins are among the more abundant proteins making up the internal nuclear matrix, but very little is known about their structure in the nucleoplasm...
  37. Maya Mendoza A, Aranda Anzaldo A. Positional mapping of specific DNA sequences relative to the nuclear substructure by direct polymerase chain reaction on nuclear matrix-bound templates. Anal Biochem. 2003;313:196-207 pubmed
    ..eukaryotes is organized in supercoiled loops anchored to a proteinaceous substructure commonly known as the nuclear matrix. Current evidence suggests that important processes of nuclear physiology, such as replication, transcription, ..
  38. Elcock L, Bridger J. Exploring the effects of a dysfunctional nuclear matrix. Biochem Soc Trans. 2008;36:1378-83 pubmed publisher
    The nuclear matrix has remained a contentious structure for decades; many believe that it is an artefact of harsh non-physiological procedures...
  39. Heng H, Goetze S, Ye C, Liu G, Stevens J, Bremer S, et al. Chromatin loops are selectively anchored using scaffold/matrix-attachment regions. J Cell Sci. 2004;117:999-1008 pubmed
    ..By utilizing FISH to visualize directly the localization of S/MARs on the nuclear matrix or chromatin loop, we were able to assign specific attributes to the S/MAR...
  40. Mearini G, Chichiarelli S, Zampieri M, Masciarelli S, D Erme M, Ferraro A, et al. Interaction of EBV latent origin of replication with the nuclear matrix: identification of S/MAR sequences and protein components. FEBS Lett. 2003;547:119-24 pubmed
    During latency, Epstein Barr virus (EBV) genome, as an episome, is attached to the nuclear matrix (NM) via the latent origin of replication ori P...
  41. Razin S, Rynditch A, Borunova V, Ioudinkova E, Smalko V, Scherrer K. The 33 kb transcript of the chicken alpha-globin gene domain is part of the nuclear matrix. J Cell Biochem. 2004;92:445-57 pubmed
    ..of alpha-globin genes is transcribed for about 33 kb in the globin direction and that this RNA is part of the nuclear matrix. Northern blot hybridisation with strand-specific riboprobes, recognising genes and intergenic sequences, and ..
  42. Moraes A, Mondin M, Beletti M, Aguiar Perecin M, Guaraldo A, Mello M. Age-related association of rDNA and telomeres with the nuclear matrix in mouse hepatocytes. Cell Biol Int. 2010;34:925-31 pubmed publisher
    Transcribed sequences have been suggested to be associated with the nuclear matrix, differing from non-transcribing sequences, which have been reported to be contained in DNA loops...
  43. Barboro P, D Arrigo C, Mormino M, Coradeghini R, Parodi S, Patrone E, et al. An intranuclear frame for chromatin compartmentalization and higher-order folding. J Cell Biochem. 2003;88:113-20 pubmed
    ..occur inside the nucleus, such as chromatin displacement and rearrangements, the collapse of the internal nuclear matrix after RNA digestion and the disruption of chromosome territories induced by RNase A and high salt treatment...
  44. Graham J, Hanson A, Croft A, Fox A, Clarke C. Nuclear matrix binding is critical for progesterone receptor movement into nuclear foci. FASEB J. 2009;23:546-56 pubmed publisher
    ..We have identified a mutation in PR that ablates its binding to the nuclear matrix and prevents PR movement into nuclear foci...
  45. Eivazova E, Markov S, Pirozhkova I, Lipinski M, Vassetzky Y. Recruitment of RNA polymerase II in the Ifng gene promoter correlates with the nuclear matrix association in activated T helper cells. J Mol Biol. 2007;371:317-22 pubmed
    ..However, the interaction of the Ifng gene promoter with the nuclear matrix occurred differentially in a lineage-specific manner...
  46. Chernov I, Akopov S, Nikolaev L. [Structure and function of nuclear matrix associated regions (S/MARs)]. Bioorg Khim. 2004;30:3-14 pubmed
    ..concepts on the chromatin loop-domain organization and the role of the DNA regions specifically binding the nuclear matrix (nuclear scaffold, or S/MARs) in its formation, maintenance, and regulation are discussed...
  47. Biggs J, Zhang Y, Peterson L, Garcia M, Zhang D, Kraft A. Phosphorylation of AML1/RUNX1 regulates its degradation and nuclear matrix association. Mol Cancer Res. 2005;3:391-401 pubmed
    ..one soluble in buffers containing salt and nonionic detergent and the other insoluble and tightly bound to the nuclear matrix. We find that the AML1c protein is modified by both phosphorylation and ubiquitination...
  48. Sayer J, Manczak M, Akileswaran L, Reddy P, Coghlan V. Interaction of the nuclear matrix protein NAKAP with HypA and huntingtin: implications for nuclear toxicity in Huntington's disease pathogenesis. Neuromolecular Med. 2005;7:297-310 pubmed
    ..In cultured cells, NAKAP and HypA localize within the nucleus and copurify with the nuclear matrix. Furthermore, NAKAP associates with HypA from human brain and copurifies with huntingtin protein in brain ..
  49. Samaniego R, Jeong S, de la Torre C, Meier I, Moreno Díaz de la Espina S. CK2 phosphorylation weakens 90 kDa MFP1 association to the nuclear matrix in Allium cepa. J Exp Bot. 2006;57:113-24 pubmed
    ..plant coiled-coil protein located on the stroma side of the chloroplast thylakoids, as well as in the nuclear matrix. It displays species-specific variability in the number of genes, proteins, and expression...
  50. Yun J, Chew E, Liew C, Chan J, Jin M, Ding M, et al. Nucleophosmin/B23 is a proliferate shuttle protein associated with nuclear matrix. J Cell Biochem. 2003;90:1140-8 pubmed
    ..It was detected in nuclear matrix protein (NMP) composition extracted from the regenerating hepatocytes and HepG2 cells and identified with ..
  51. Codrington A, Hales B, Robaire B. Chronic cyclophosphamide exposure alters the profile of rat sperm nuclear matrix proteins. Biol Reprod. 2007;77:303-11 pubmed
    ..Sperm DNA is organized by the nuclear matrix into loop-domains in a sequence-specific manner...
  52. Mesner L, Hamlin J, Dijkwel P. The matrix attachment region in the Chinese hamster dihydrofolate reductase origin of replication may be required for local chromatid separation. Proc Natl Acad Sci U S A. 2003;100:3281-6 pubmed
    Centered in the Chinese hamster dihydrofolate reductase origin of replication is a prominent nuclear matrix attachment region (MAR)...
  53. Taniura H, Kobayashi M, Yoshikawa K. Functional domains of necdin for protein-protein interaction, nuclear matrix targeting, and cell growth suppression. J Cell Biochem. 2005;94:804-15 pubmed
    ..We here characterize the regions of necdin required for the protein-protein interaction, nuclear matrix targeting, and cell growth suppression...