coiled bodies

Summary

Summary: A distinct subnuclear domain enriched in splicesomal snRNPs (RIBONUCLEOPROTEINS, SMALL NUCLEAR) and p80-coilin.

Top Publications

  1. Gall J. The centennial of the Cajal body. Nat Rev Mol Cell Biol. 2003;4:975-80 pubmed publisher
  2. Dundr M, Misteli T. Functional architecture in the cell nucleus. Biochem J. 2001;356:297-310 pubmed
    ..Finally, we speculate that self-organization might play a substantial role in establishing and maintaining nuclear organization...
  3. Kim S, Ryabov E, Kalinina N, Rakitina D, Gillespie T, MacFarlane S, et al. Cajal bodies and the nucleolus are required for a plant virus systemic infection. EMBO J. 2007;26:2169-79 pubmed
    ..We provide a model whereby the ORF3 protein utilizes trafficking pathways involving CBs to enter the nucleolus and, along with fibrillarin, exit the nucleus to form viral 'transport-competent' RNP particles in the cytoplasm...
  4. Girard C, Neel H, Bertrand E, Bordonné R. Depletion of SMN by RNA interference in HeLa cells induces defects in Cajal body formation. Nucleic Acids Res. 2006;34:2925-32 pubmed
    ..Because Cajal bodies represent the location in which snRNPs undergo 2'-O-methylation and pseudouridylation, our results raise the possibility that SMN depletion might give rise to a defect in the snRNA modification process...
  5. Batalova F, Stepanova I, Skovorodkin I, Bogolyubov D, Parfenov V. Identification and dynamics of Cajal bodies in relation to karyosphere formation in scorpionfly oocytes. Chromosoma. 2005;113:428-39 pubmed
  6. Carnegie G, Sleeman J, Morrice N, Hastie C, Peggie M, Philp A, et al. Protein phosphatase 4 interacts with the Survival of Motor Neurons complex and enhances the temporal localisation of snRNPs. J Cell Sci. 2003;116:1905-13 pubmed
    ..Expression of GFP-R2-PPP4c in HeLa cells enhances the temporal localisation of newly formed snRNPs, which is consistent with an association of R2-PPP4c with the SMN protein complex...
  7. Matera A. Drosophila Cajal bodies: accessories not included. J Cell Biol. 2006;172:791-3 pubmed
    ..A recent study describes the discovery of the Drosophila Cajal body, revealing some interesting insights into the subnuclear organization of RNA processing machineries among different species...
  8. Lemos T, Kobarg J. CGI-55 interacts with nuclear proteins and co-localizes to p80-coilin positive-coiled bodies in the nucleus. Cell Biochem Biophys. 2006;44:463-74 pubmed
    ..However, we observed that CGI-55 localizes to the nucleolus and co-localizes with p80-coilin positive nuclear-coiled bodies.
  9. Li C, Pontes O, El Shami M, Henderson I, Bernatavichute Y, Chan S, et al. An ARGONAUTE4-containing nuclear processing center colocalized with Cajal bodies in Arabidopsis thaliana. Cell. 2006;126:93-106 pubmed
    ..Our results suggest a function for Cajal bodies as a center for the assembly of an AGO4/NRPD1b/siRNA complex, facilitating its function in RNA-directed gene silencing at target loci...

More Information

Publications62

  1. Machyna M, Heyn P, Neugebauer K. Cajal bodies: where form meets function. Wiley Interdiscip Rev RNA. 2013;4:17-34 pubmed publisher
    ..CB assembly encompasses an additional layer of complexity, whereby a set of pre-existing substructures can be integrated into mature CBs. We propose this as a multi-seeding model of CB assembly...
  2. Yang J, Baserga S, Turley S, Pollard K. Fibrillarin and other snoRNP proteins are targets of autoantibodies in xenobiotic-induced autoimmunity. Clin Immunol. 2001;101:38-50 pubmed
    ..These studies suggest that the mercury-induced anti-nucleolar antibody response targets other protein components of the snoRNP particles in addition to fibrillarin...
  3. Cauchi R. SMN and Gemins: 'we are family' … or are we?: insights into the partnership between Gemins and the spinal muscular atrophy disease protein SMN. Bioessays. 2010;32:1077-89 pubmed publisher
    ..This review evaluates the functions of Gemins and UNRIP inside the SMN complex and discusses whether these less notorious SMN complex members are capable of acting independently of SMN...
  4. Hearst S, Gilder A, Negi S, Davis M, George E, Whittom A, et al. Cajal-body formation correlates with differential coilin phosphorylation in primary and transformed cell lines. J Cell Sci. 2009;122:1872-81 pubmed publisher
    ..Taken together, our data support a model whereby coilin is the target of an uncharacterized signal transduction cascade that responds to the increased transcription and snRNP demands found in transformed cells...
  5. Boisvert F, Cote J, Boulanger M, Cleroux P, Bachand F, Autexier C, et al. Symmetrical dimethylarginine methylation is required for the localization of SMN in Cajal bodies and pre-mRNA splicing. J Cell Biol. 2002;159:957-69 pubmed
    ..Our findings support a model in which arginine methylation is important for the localization of coilin and SMN in Cajal bodies...
  6. Gilder A, Do P, Carrero Z, Cosman A, Broome H, Velma V, et al. Coilin participates in the suppression of RNA polymerase I in response to cisplatin-induced DNA damage. Mol Biol Cell. 2011;22:1070-9 pubmed publisher
    ..Our findings identify a novel and unexpected function for coilin, independent of its role in snRNP biogenesis, establishing a new link between the DNA damage response and the inhibition of rRNA synthesis...
  7. Ospina J, Gonsalvez G, Bednenko J, Darzynkiewicz E, Gerace L, Matera A. Cross-talk between snurportin1 subdomains. Mol Biol Cell. 2005;16:4660-71 pubmed
    ..SPN also relocalizes to Cajal bodies upon treatment with leptomycin B. Finally, we uncovered an interaction between the N- and C-terminal domains of SPN, suggesting an autoregulatory function similar to that of importin-alpha...
  8. Gall J. A role for Cajal bodies in assembly of the nuclear transcription machinery. FEBS Lett. 2001;498:164-7 pubmed
    ..It is suggested that pol I, pol II, and pol III transcription and processing complexes are preassembled in the CBs before transport to the sites of transcription on the chromosomes and in the nucleoli...
  9. Stanek D, Rader S, Klingauf M, Neugebauer K. Targeting of U4/U6 small nuclear RNP assembly factor SART3/p110 to Cajal bodies. J Cell Biol. 2003;160:505-16 pubmed
    ..We propose that U4 and U6 snRNPs accumulate in CBs for the purpose of assembly into U4/U6 snRNPs by SART3/p110...
  10. Zatsepina O, Baly C, Chebrout M, Debey P. The step-wise assembly of a functional nucleolus in preimplantation mouse embryos involves the cajal (coiled) body. Dev Biol. 2003;253:66-83 pubmed
    ..We also find that fibrillarin and B23 are both directly assembled around NPBs prior to ongoing pre-rRNA synthesis. Altogether, our results reveal a role of the Cajal bodies in the building of a functional nucleolus...
  11. Gerbi S, Lange T. All small nuclear RNAs (snRNAs) of the [U4/U6.U5] Tri-snRNP localize to nucleoli; Identification of the nucleolar localization element of U6 snRNA. Mol Biol Cell. 2002;13:3123-37 pubmed
    ..Conversion of the 3' hydroxyl of U6 snRNA to a 3' phosphate prevents association with the La protein but does not affect U6 localization to nucleoli or Cajal bodies...
  12. Collier S, Pendle A, Boudonck K, van Rij T, Dolan L, Shaw P. A distant coilin homologue is required for the formation of cajal bodies in Arabidopsis. Mol Biol Cell. 2006;17:2942-51 pubmed
    ..The Atcoilin gene is unaffected in pcb and ccb, suggesting that other genes can also affect CBs...
  13. Deryusheva S, Gall J. Small Cajal body-specific RNAs of Drosophila function in the absence of Cajal bodies. Mol Biol Cell. 2009;20:5250-9 pubmed publisher
    ..We propose that the snRNA modification machinery is not limited to CBs, but is dispersed throughout the nucleoplasm of cells in general...
  14. Shaw P, Brown J. Plant nuclear bodies. Curr Opin Plant Biol. 2004;7:614-20 pubmed
    ..These novel domains illustrate the complexity and subtlety of expression control and herald a new era of research on the molecular and cell biology of plant nuclei...
  15. Clelland A, Kinnear N, Oram L, Burza J, Sleeman J. The SMN protein is a key regulator of nuclear architecture in differentiating neuroblastoma cells. Traffic. 2009;10:1585-98 pubmed publisher
    ..This suggests different mechanisms are present in different cell types and in vivo that may be significant for the tissue-specific pathology of SMA...
  16. Cioce M, Boulon S, Matera A, Lamond A. UV-induced fragmentation of Cajal bodies. J Cell Biol. 2006;175:401-13 pubmed
    ..These data demonstrate that CBs are specific nuclear targets of cellular stress-response pathways and identify PA28gamma as a novel regulator of CB integrity...
  17. Sleeman J, Lamond A. Newly assembled snRNPs associate with coiled bodies before speckles, suggesting a nuclear snRNP maturation pathway. Curr Biol. 1999;9:1065-74 pubmed
    ..These include speckles, coiled bodies and nucleoli, in addition to a diffuse nucleoplasmic compartment...
  18. Stanek D, Neugebauer K. Detection of snRNP assembly intermediates in Cajal bodies by fluorescence resonance energy transfer. J Cell Biol. 2004;166:1015-25 pubmed
    ..U4/U6 snRNP in CBs. Thus, while some U4/U6 snRNP assembly can occur in the nucleoplasm, these data provide evidence that SART3.U6 snRNPs form in the nucleoplasm and translocate to CBs where U4/U6 snRNP assembly occurs...
  19. Tomlinson R, Abreu E, Ziegler T, Ly H, Counter C, Terns R, et al. Telomerase reverse transcriptase is required for the localization of telomerase RNA to cajal bodies and telomeres in human cancer cells. Mol Biol Cell. 2008;19:3793-800 pubmed publisher
    ..Our findings suggest that the trafficking of telomerase to Cajal bodies and telomeres in cancer cells correlates with and depends on the assembly of the enzyme...
  20. Zhu Y, Tomlinson R, Lukowiak A, Terns R, Terns M. Telomerase RNA accumulates in Cajal bodies in human cancer cells. Mol Biol Cell. 2004;15:81-90 pubmed
    ..Moreover, we report that hTERT is also found in Cajal bodies. Our data suggest that Cajal bodies are involved in the assembly and/or function of human telomerase...
  21. Leung A, Lamond A. In vivo analysis of NHPX reveals a novel nucleolar localization pathway involving a transient accumulation in splicing speckles. J Cell Biol. 2002;157:615-29 pubmed
    ..The data show a specific temporal pathway involving the sequential and directed accumulation of NHPX in distinct subnuclear compartments, and define a novel mechanism for nucleolar localization...
  22. Darzacq X, Jady B, Verheggen C, Kiss A, Bertrand E, Kiss T. Cajal body-specific small nuclear RNAs: a novel class of 2'-O-methylation and pseudouridylation guide RNAs. EMBO J. 2002;21:2746-56 pubmed
    ..Our results provide strong support for the idea that the Cajal body, this mysterious nuclear organelle, provides the cellular locale for post-transcriptional modification of spliceosomal snRNAs...
  23. Nesic D, Tanackovic G, Kramer A. A role for Cajal bodies in the final steps of U2 snRNP biogenesis. J Cell Sci. 2004;117:4423-33 pubmed
    ..By contrast, SF3a60 and SF3a66 lacking the zinc finger domains accumulate in Cajal bodies and are diffusely distributed in the cytoplasm, suggesting a function for Cajal bodies in the final maturation of the U2 snRNP...
  24. Young P, Le T, thi Man N, Burghes A, Morris G. The relationship between SMN, the spinal muscular atrophy protein, and nuclear coiled bodies in differentiated tissues and cultured cells. Exp Cell Res. 2000;256:365-74 pubmed
    ..quot; Gems are closely associated with nuclear coiled bodies and both may have a direct role in snRNP maturation and pre-RNA splicing...
  25. James N, Howell G, Walker J, Blair G. The role of Cajal bodies in the expression of late phase adenovirus proteins. Virology. 2010;399:299-311 pubmed publisher
    ..We conclude that CBs have an important role in the expression of adenovirus major late gene products...
  26. Toyota C, Davis M, Cosman A, Hebert M. Coilin phosphorylation mediates interaction with SMN and SmB'. Chromosoma. 2010;119:205-15 pubmed publisher
    ..These data show that phosphorylation of coilin influences interaction with its target proteins and, thus, may be significant in managing the flow of snRNPs through the CB...
  27. Verheggen C, Mouaikel J, Thiry M, Blanchard J, Tollervey D, Bordonne R, et al. Box C/D small nucleolar RNA trafficking involves small nucleolar RNP proteins, nucleolar factors and a novel nuclear domain. EMBO J. 2001;20:5480-90 pubmed
    ..We conclude that snoRNP assembly occurs either in the nucleoplasm, or during transit of snoRNAs through the NB, followed by routing of the complete snoRNP to functional sites of ribosome synthesis...
  28. Song L, Han M, Lesicka J, Fedoroff N. Arabidopsis primary microRNA processing proteins HYL1 and DCL1 define a nuclear body distinct from the Cajal body. Proc Natl Acad Sci U S A. 2007;104:5437-42 pubmed
    ..Thus, the distinct HYL1- and DCL1-containing nuclear bodies may be miRNA precursor processing sites. Alternatively, they may be assembly and storage sites for the miRNA precursor processing machinery...
  29. Filipowicz W, Pogacic V. Biogenesis of small nucleolar ribonucleoproteins. Curr Opin Cell Biol. 2002;14:319-27 pubmed
    ..Cajal bodies emerge as nuclear structures important for the biogenesis and function of small nucleolar ribonucleoproteins...
  30. Bubulya P, Spector D. "On the move"ments of nuclear components in living cells. Exp Cell Res. 2004;296:4-11 pubmed
    ..In this review we focus on recent advances related to the dynamics of chromatin domains, as well as the dynamics of nuclear proteins and several nuclear organelles...
  31. Narayanan U, Ospina J, Frey M, Hebert M, Matera A. SMN, the spinal muscular atrophy protein, forms a pre-import snRNP complex with snurportin1 and importin beta. Hum Mol Genet. 2002;11:1785-95 pubmed
    ..Therefore, we conclude that, following Sm protein assembly, the SMN complex persists until the final stages of cytoplasmic snRNP maturation and may provide somatic cell RNPs with an alternative NLS...
  32. Xie S, Pombo A. Distribution of different phosphorylated forms of RNA polymerase II in relation to Cajal and PML bodies in human cells: an ultrastructural study. Histochem Cell Biol. 2006;125:21-31 pubmed
    ..These results support the view that although PML bodies are present in transcriptionally active areas of the nucleus, they are not generally sites of polymerase II assembly, transport or activity...
  33. Wei Y, Jin J, Harper J. The cyclin E/Cdk2 substrate and Cajal body component p220(NPAT) activates histone transcription through a novel LisH-like domain. Mol Cell Biol. 2003;23:3669-80 pubmed
    ..These studies indicate that the ability of p220 to promote S phase is independent of its ability to promote histone H4 transcription and suggests that p220 may link cyclin E/Cdk2 to multiple independent downstream functions...
  34. Lafarga M, Casafont I, Bengoechea R, Tapia O, Berciano M. Cajal's contribution to the knowledge of the neuronal cell nucleus. Chromosoma. 2009;118:437-43 pubmed publisher
    ..The aim of this article is to revisit Cajal's contributions to the knowledge of the neuronal nucleus in light of our current understanding of nuclear structure and function...
  35. Tucker K, Massello L, Gao L, Barber T, Hebert M, Chan E, et al. Structure and characterization of the murine p80 coilin gene, Coil. J Struct Biol. 2000;129:269-77 pubmed
    Cajal bodies (coiled bodies, CBs) are nuclear organelles of unknown function and are characterized by a wide variety of components including various basal transcription and cell cycle proteins, the nucleolar proteins fibrillarin and ..
  36. Shan X, Chiang P, Price D, Wong P. Altered distributions of Gemini of coiled bodies and mitochondria in motor neurons of TDP-43 transgenic mice. Proc Natl Acad Sci U S A. 2010;107:16325-30 pubmed publisher
    ..level of TDP-43 disrupts the normal nuclear distribution of survival motor neuron (SMN)-associated Gemini of coiled bodies (GEMs) in motor neurons, its absence prevents the formation of GEMs in the nuclei of these cells...
  37. Nizami Z, Deryusheva S, Gall J. The Cajal body and histone locus body. Cold Spring Harb Perspect Biol. 2010;2:a000653 pubmed publisher
    ..CBs and HLBs are present throughout the interphase of the cell cycle, but disappear during mitosis. The biogenesis of CBs shows the features of a self-organizing structure...
  38. Kiss T, Fayet Lebaron E, Jady B. Box H/ACA small ribonucleoproteins. Mol Cell. 2010;37:597-606 pubmed publisher
    ..Recently, great progress has been made toward understanding of the biogenesis, intracellular trafficking, structure, and function of H/ACA RNPs...
  39. Jady B, Darzacq X, Tucker K, Matera A, Bertrand E, Kiss T. Modification of Sm small nuclear RNAs occurs in the nucleoplasmic Cajal body following import from the cytoplasm. EMBO J. 2003;22:1878-88 pubmed
    ..In coilin knockout cells, Sm snRNAs and their modification guide RNAs colocalize in residual CBs, which do not stockpile fibrillarin and fail to recruit the U3 small nucleolar RNA...
  40. Matera A, Shpargel K. Pumping RNA: nuclear bodybuilding along the RNP pipeline. Curr Opin Cell Biol. 2006;18:317-24 pubmed
  41. Kaiser T, Intine R, Dundr M. De novo formation of a subnuclear body. Science. 2008;322:1713-7 pubmed publisher
    ..Biogenesis of the CB does not follow a hierarchical assembly pathway and exhibits hallmarks of a self-organizing structure...
  42. Grimmler M, Otter S, Peter C, Müller F, Chari A, Fischer U. Unrip, a factor implicated in cap-independent translation, associates with the cytosolic SMN complex and influences its intracellular localization. Hum Mol Genet. 2005;14:3099-111 pubmed
    ..We speculate that unrip plays a crucial role in the intracellular distribution of the SMN complex...
  43. Verheggen C, Lafontaine D, Samarsky D, Mouaikel J, Blanchard J, Bordonné R, et al. Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments. EMBO J. 2002;21:2736-45 pubmed
    ..These are likely places for snoRNP assembly, 3' end maturation and cap modification...
  44. Tycowski K, Shu M, Kukoyi A, Steitz J. A conserved WD40 protein binds the Cajal body localization signal of scaRNP particles. Mol Cell. 2009;34:47-57 pubmed publisher
    ..We demonstrate a requirement for WDR79 binding in the CB localization of a scaRNA. This and other recent reports establish WDR79 as a central player in the localization and processing of nuclear RNPs...
  45. Hebert M, Szymczyk P, Shpargel K, Matera A. Coilin forms the bridge between Cajal bodies and SMN, the spinal muscular atrophy protein. Genes Dev. 2001;15:2720-9 pubmed
    ..A cardinal feature of SMA patient cells is a defect in the targeting of SMN to nuclear foci; our results uncover a role for coilin in this process...
  46. Straatman K, Schel J. Distribution of splicing proteins and putative coiled bodies during pollen development and androgenesis in Brassica napus L. Protoplasma. 2001;216:191-200 pubmed
    ..The snRNP-positive nuclei contained mostly one or two brightly labelled nuclear bodies, most likely coiled bodies, often closely related to the nucleolus. These nuclear bodies increased in size from 0...
  47. Frey M, Matera A. RNA-mediated interaction of Cajal bodies and U2 snRNA genes. J Cell Biol. 2001;154:499-509 pubmed
    ..Our data thus provide significant insight into the mechanism of CB interaction with snRNA loci, strengthening the putative role for this nuclear suborganelle in snRNP biogenesis...
  48. Richard P, Darzacq X, Bertrand E, Jady B, Verheggen C, Kiss T. A common sequence motif determines the Cajal body-specific localization of box H/ACA scaRNAs. EMBO J. 2003;22:4283-93 pubmed
    ..Our results indicate that scaRNAs represent an ancient group of small nuclear RNAs which are localized to Cajal bodies by an evolutionarily conserved mechanism...
  49. Kiss T, Fayet E, Jády B, Richard P, Weber M. Biogenesis and intranuclear trafficking of human box C/D and H/ACA RNPs. Cold Spring Harb Symp Quant Biol. 2006;71:407-17 pubmed
    ..This suggests that a cell-cycle-dependent, dynamic localization of hTR to telomeres may play an important regulatory role in human telomere synthesis...
  50. Klingauf M, Stanek D, Neugebauer K. Enhancement of U4/U6 small nuclear ribonucleoprotein particle association in Cajal bodies predicted by mathematical modeling. Mol Biol Cell. 2006;17:4972-81 pubmed
    ..This provides a chemical foundation for the proposal that these and other cellular compartments promote molecular interactions, by increasing the local concentration of individual components...
  51. Tucker K, Berciano M, Jacobs E, LePage D, Shpargel K, Rossire J, et al. Residual Cajal bodies in coilin knockout mice fail to recruit Sm snRNPs and SMN, the spinal muscular atrophy gene product. J Cell Biol. 2001;154:293-307 pubmed
  52. Handwerger K, Murphy C, Gall J. Steady-state dynamics of Cajal body components in the Xenopus germinal vesicle. J Cell Biol. 2003;160:495-504 pubmed
    ..We propose that binding to other molecules and possibly assembly into larger complexes are the rate-limiting steps for FRAP of U7, coilin, and TBP inside CBs...
  53. Platani M, Goldberg I, Lamond A, Swedlow J. Cajal body dynamics and association with chromatin are ATP-dependent. Nat Cell Biol. 2002;4:502-8 pubmed
    ..This behaviour is fundamentally different from the ATP-dependent mobility observed for chromatin and suggests that a novel mechanism governs CB, and possibly other, nuclear body dynamics...