intermediate filaments

Summary

Summary: Cytoplasmic filaments intermediate in diameter (about 10 nanometers) between the microfilaments and the microtubules. They may be composed of any of a number of different proteins and form a ring around the cell nucleus.

Top Publications

  1. Herrmann H, Strelkov S, Burkhard P, Aebi U. Intermediate filaments: primary determinants of cell architecture and plasticity. J Clin Invest. 2009;119:1772-83 pubmed publisher
    b>Intermediate filaments (IFs) are major constituents of the cytoskeleton and nuclear boundary in animal cells. They are of prime importance for the functional organization of structural elements...
  2. Gregor M, Osmanagic Myers S, Burgstaller G, Wolfram M, Fischer I, Walko G, et al. Mechanosensing through focal adhesion-anchored intermediate filaments. FASEB J. 2014;28:715-29 pubmed publisher
    ..Here, we show a hitherto unrecognized regulatory role of vimentin intermediate filaments (IFs) in this process...
  3. FitzGerald P. Lens intermediate filaments. Exp Eye Res. 2009;88:165-72 pubmed publisher
    ..Some of the key features of both filament systems are reviewed...
  4. Song S, Landsbury A, Dahm R, Liu Y, Zhang Q, Quinlan R. Functions of the intermediate filament cytoskeleton in the eye lens. J Clin Invest. 2009;119:1837-48 pubmed publisher
    b>Intermediate filaments (IFs) are a key component of the cytoskeleton in virtually all vertebrate cells, including those of the lens of the eye. IFs help integrate individual cells into their respective tissues...
  5. Chang L, Barlan K, Chou Y, Grin B, Lakonishok M, Serpinskaya A, et al. The dynamic properties of intermediate filaments during organelle transport. J Cell Sci. 2009;122:2914-23 pubmed publisher
    ..These results reveal a dynamic interaction between membrane bound pigment granules and IFs and suggest a role for IFs as modulators of granule movement...
  6. Colin C, Virard I, Baeza N, Tchoghandjian A, Fernandez C, Bouvier C, et al. Relevance of combinatorial profiles of intermediate filaments and transcription factors for glioma histogenesis. Neuropathol Appl Neurobiol. 2007;33:431-9 pubmed
    ..Finally, in oligodendrogliomas, intermediate filament proteins are generally not observed whereas Olig2 was found in almost all tumour cells nuclei while only a subpopulation of tumour cells expressed Nkx2.2 and Sox10...
  7. Klauke B, Kossmann S, Gaertner A, Brand K, Stork I, Brodehl A, et al. De novo desmin-mutation N116S is associated with arrhythmogenic right ventricular cardiomyopathy. Hum Mol Genet. 2010;19:4595-607 pubmed publisher
    ..Thus, the gene coding for desmin appears to be a novel ARVC gene, which should be included in molecular genetic screening of ARVC patients...
  8. Liu T, Guevara O, Warburton R, Hill N, Gaestel M, Kayyali U. Regulation of vimentin intermediate filaments in endothelial cells by hypoxia. Am J Physiol Cell Physiol. 2010;299:C363-73 pubmed publisher
    ..b>Intermediate filaments are major components of the cytoskeleton whose contribution to endothelial physiology is not well ..
  9. Green K, Getsios S, Troyanovsky S, Godsel L. Intercellular junction assembly, dynamics, and homeostasis. Cold Spring Harb Perspect Biol. 2010;2:a000125 pubmed publisher
    ..junctions-adherens junctions (AJs) and desmosomes (DSMs), which associate with actin and intermediate filaments, respectively...

More Information

Publications62

  1. Lee C, Coulombe P. Self-organization of keratin intermediate filaments into cross-linked networks. J Cell Biol. 2009;186:409-21 pubmed publisher
    ..We propose that self-organization is a key determinant of the structural support function of keratin IFs in vivo...
  2. Zhang H, Landmann F, Zahreddine H, Rodriguez D, Koch M, Labouesse M. A tension-induced mechanotransduction pathway promotes epithelial morphogenesis. Nature. 2011;471:99-103 pubmed publisher
    ..The phosphorylation of intermediate filaments is one output of this pathway...
  3. Dupin I, Sakamoto Y, Etienne Manneville S. Cytoplasmic intermediate filaments mediate actin-driven positioning of the nucleus. J Cell Sci. 2011;124:865-72 pubmed publisher
    ..We show here that cytoplasmic intermediate filaments are essential players in actin-dependent positioning of the nucleus...
  4. Eriksson J, Dechat T, Grin B, Helfand B, Mendez M, Pallari H, et al. Introducing intermediate filaments: from discovery to disease. J Clin Invest. 2009;119:1763-71 pubmed publisher
    It took more than 100 years before it was established that the proteins that form intermediate filaments (IFs) comprise a unified protein family, the members of which are ubiquitous in virtually all differentiated cells and present both ..
  5. Phua D, Humbert P, Hunziker W. Vimentin regulates scribble activity by protecting it from proteasomal degradation. Mol Biol Cell. 2009;20:2841-55 pubmed publisher
    ..Thus, the upregulation of vimentin expression during epithelial to mesenchymal transitions may stabilize Scrib to promote directed cell migration. ..
  6. Cabeen M, Charbon G, Vollmer W, Born P, Ausmees N, Weibel D, et al. Bacterial cell curvature through mechanical control of cell growth. EMBO J. 2009;28:1208-19 pubmed publisher
    ..Our study suggests that bacteria may use the cytoskeleton for mechanical control of growth to alter morphology...
  7. Long H, Boczonadi V, McInroy L, Goldberg M, Määttä A. Periplakin-dependent re-organisation of keratin cytoskeleton and loss of collective migration in keratin-8-downregulated epithelial sheets. J Cell Sci. 2006;119:5147-59 pubmed
    ..We have investigated the role of keratin intermediate filaments and periplakin, a cytoskeletal linker protein, in the migration of simple epithelial cells...
  8. Kumar Y, Valdivia R. Actin and intermediate filaments stabilize the Chlamydia trachomatis vacuole by forming dynamic structural scaffolds. Cell Host Microbe. 2008;4:159-69 pubmed publisher
    ..the inclusion is encased in a scaffold of host cytoskeletal structures made up of a network of F-actin and intermediate filaments (IF) that act cooperatively to stabilize the pathogen-containing vacuole...
  9. Jaitovich A, Mehta S, Na N, Ciechanover A, Goldman R, Ridge K. Ubiquitin-proteasome-mediated degradation of keratin intermediate filaments in mechanically stimulated A549 cells. J Biol Chem. 2008;283:25348-55 pubmed publisher
    We previously reported that shear stress induces phosphorylation and disassembly of keratin intermediate filaments (IFs)...
  10. Godsel L, Hobbs R, Green K. Intermediate filament assembly: dynamics to disease. Trends Cell Biol. 2008;18:28-37 pubmed
    ..We end by discussing how mutations leading to defects in IF assembly, network formation or IF-IFAP association compromise in vivo functions of IF as protectors against environmental stress...
  11. Magin T, Vijayaraj P, Leube R. Structural and regulatory functions of keratins. Exp Cell Res. 2007;313:2021-32 pubmed
    ..All of these properties are controlled by highly complex patterns of phosphorylation and molecular associations...
  12. Goodall M, Ward C, Pratt S, Bloch R, Lovering R. Structural and functional evaluation of branched myofibers lacking intermediate filaments. Am J Physiol Cell Physiol. 2012;303:C224-32 pubmed publisher
    b>Intermediate filaments (IFs), composed of desmin and keratins, link myofibrils to each other and to the sarcolemma in skeletal muscle...
  13. Mashukova A, Oriolo A, Wald F, Casanova M, Kröger C, Magin T, et al. Rescue of atypical protein kinase C in epithelia by the cytoskeleton and Hsp70 family chaperones. J Cell Sci. 2009;122:2491-503 pubmed publisher
    ..We conclude that both filamentous keratins and Hsp70 are required for the rescue rephosphorylation of mature atypical PKC, regulating the subcellular distribution and steady-state levels of active PKC iota...
  14. Pan X, Hobbs R, Coulombe P. The expanding significance of keratin intermediate filaments in normal and diseased epithelia. Curr Opin Cell Biol. 2013;25:47-56 pubmed publisher
    b>Intermediate filaments are assembled from a diverse group of evolutionary conserved proteins and are specified in a tissue-dependent, cell type-dependent, and context-dependent fashion in the body...
  15. Colakoglu G, Brown A. Intermediate filaments exchange subunits along their length and elongate by end-to-end annealing. J Cell Biol. 2009;185:769-77 pubmed publisher
    ..shorten by addition and loss of subunits at their ends, but it is not known whether this is also true for intermediate filaments. In fact, several studies suggest that in vivo, intermediate filaments may lengthen by end-to-end ..
  16. Chou Y, Flitney F, Chang L, Mendez M, Grin B, Goldman R. The motility and dynamic properties of intermediate filaments and their constituent proteins. Exp Cell Res. 2007;313:2236-43 pubmed
    ..The recent findings that IF and their precursors are frequently associated with signaling molecules have revealed new functions for IF beyond their more traditional roles as mechanical integrators of cells and tissues...
  17. Qin Z, Kreplak L, Buehler M. Hierarchical structure controls nanomechanical properties of vimentin intermediate filaments. PLoS ONE. 2009;4:e7294 pubmed publisher
    b>Intermediate filaments (IFs), in addition to microtubules and microfilaments, are one of the three major components of the cytoskeleton in eukaryotic cells, playing a vital role in mechanotransduction and in providing mechanical stability ..
  18. Bhattacharya R, Gonzalez A, DeBiase P, Trejo H, Goldman R, Flitney F, et al. Recruitment of vimentin to the cell surface by beta3 integrin and plectin mediates adhesion strength. J Cell Sci. 2009;122:1390-400 pubmed publisher
    ..However, vimentin intermediate filaments (IFs) also associate with the cell surface at FAs in endothelial cells...
  19. Valencia R, Walko G, Janda L, Novacek J, Mihailovska E, Reipert S, et al. Intermediate filament-associated cytolinker plectin 1c destabilizes microtubules in keratinocytes. Mol Biol Cell. 2013;24:768-84 pubmed publisher
    ..While MT dynamics are often linked to those of actin filaments, little is known about whether intermediate filaments (IFs) have an influence on MT dynamics...
  20. Windoffer R, Beil M, Magin T, Leube R. Cytoskeleton in motion: the dynamics of keratin intermediate filaments in epithelia. J Cell Biol. 2011;194:669-78 pubmed publisher
    Epithelia are exposed to multiple forms of stress. Keratin intermediate filaments are abundant in epithelia and form cytoskeletal networks that contribute to cell type-specific functions, such as adhesion, migration, and metabolism...
  21. Pallari H, Eriksson J. Intermediate filaments as signaling platforms. Sci STKE. 2006;2006:pe53 pubmed
    b>Intermediate filaments (IFs) are cytoskeletal structures that are crucial for maintaining the structural and mechanical integrity of cells and tissues...
  22. Leech S, Evans C, Shaw L, Wong C, Connolly J, Griffiths J, et al. Proteomic analyses of intermediate filaments reveals cytokeratin8 is highly acetylated--implications for colorectal epithelial homeostasis. Proteomics. 2008;8:279-88 pubmed publisher
    ..Cytokeratins (CKs) have roles in cytoskeletal function as components of the intermediate filaments (IFs) and this involves CKs in the regulation of tissue homeostasis of high-turnover epithelia such as the ..
  23. John McKinnon A, Harland D. A concerted polymerization-mesophase separation model for formation of trichocyte intermediate filaments and macrofibril templates. 1: relating phase separation to structural development. J Struct Biol. 2011;173:229-40 pubmed publisher
    ..matrix proteins in trichokeratins, are formed by intracellular anisotropic phase separation of intermediate filaments (IFs), is here developed in detail...
  24. Sandén C, Broselid S, Cornmark L, Andersson K, Daszkiewicz Nilsson J, Mårtensson U, et al. G protein-coupled estrogen receptor 1/G protein-coupled receptor 30 localizes in the plasma membrane and traffics intracellularly on cytokeratin intermediate filaments. Mol Pharmacol. 2011;79:400-10 pubmed publisher
    ..One such intracellular structure was identified as cytokeratin (CK) intermediate filaments, including those composed of CK7 and CK8, but apparently not endoplasmic reticulum, Golgi, or microtubules...
  25. Favre B, Schneider Y, Lingasamy P, Bouameur J, Begre N, Gontier Y, et al. Plectin interacts with the rod domain of type III intermediate filament proteins desmin and vimentin. Eur J Cell Biol. 2011;90:390-400 pubmed publisher
    ..Moreover, binding of plectin to the conserved rod domain of IF proteins could well explain its broad interaction with most types of IFs...
  26. Lovering R, O Neill A, Muriel J, Prosser B, Strong J, Bloch R. Physiology, structure, and susceptibility to injury of skeletal muscle in mice lacking keratin 19-based and desmin-based intermediate filaments. Am J Physiol Cell Physiol. 2011;300:C803-13 pubmed publisher
    b>Intermediate filaments, composed of desmin and of keratins, play important roles in linking contractile elements to each other and to the sarcolemma in striated muscle...
  27. Parry D, Strelkov S, Burkhard P, Aebi U, Herrmann H. Towards a molecular description of intermediate filament structure and assembly. Exp Cell Res. 2007;313:2204-16 pubmed
    b>Intermediate filaments (IFs) represent one of the prominent cytoskeletal elements of metazoan cells...
  28. Perrot R, Eyer J. Neuronal intermediate filaments and neurodegenerative disorders. Brain Res Bull. 2009;80:282-95 pubmed publisher
    b>Intermediate filaments represent the most abundant cytoskeletal element in mature neurons. Mutations and/or accumulations of neuronal intermediate filament proteins are frequently observed in several human neurodegenerative disorders...
  29. Sakamoto Y, Boëda B, Etienne Manneville S. APC binds intermediate filaments and is required for their reorganization during cell migration. J Cell Biol. 2013;200:249-58 pubmed publisher
    b>Intermediate filaments (IFs) are components of the cytoskeleton involved in most cellular functions, including cell migration...
  30. Spurny R, Gregor M, Castañón M, Wiche G. Plectin deficiency affects precursor formation and dynamics of vimentin networks. Exp Cell Res. 2008;314:3570-80 pubmed publisher
    Plectin is a typical cytolinker protein that connects intermediate filaments to the other cytoskeletal filament systems and anchors them at membrane-associated junctional sites...
  31. Strnad P, Stumptner C, Zatloukal K, Denk H. Intermediate filament cytoskeleton of the liver in health and disease. Histochem Cell Biol. 2008;129:735-49 pubmed publisher
    b>Intermediate filaments (IFs) represent the largest cytoskeletal gene family comprising approximately 70 genes expressed in tissue specific manner...
  32. Tang D. Intermediate filaments in smooth muscle. Am J Physiol Cell Physiol. 2008;294:C869-78 pubmed publisher
    ..This review also summarizes our latest understanding on how the IF network may be regulated in smooth muscle...
  33. Carberry K, Wiesenfahrt T, Windoffer R, Bossinger O, Leube R. Intermediate filaments in Caenorhabditis elegans. Cell Motil Cytoskeleton. 2009;66:852-64 pubmed publisher
    b>Intermediate filaments (IFs) make up one of the three major fibrous cytoskeletal systems in metazoans. Numerous IF polypeptides are synthesized in cell type-specific combinations suggesting specialized functions...
  34. Charbon G, Cabeen M, Jacobs Wagner C. Bacterial intermediate filaments: in vivo assembly, organization, and dynamics of crescentin. Genes Dev. 2009;23:1131-44 pubmed publisher
    ..Additionally, analysis of an MreB localization mutant suggests that cell wall insertion during cell elongation normally occurs along two helices of opposite handedness, each counterbalancing the other's torque...
  35. Herrmann H, Bär H, Kreplak L, Strelkov S, Aebi U. Intermediate filaments: from cell architecture to nanomechanics. Nat Rev Mol Cell Biol. 2007;8:562-73 pubmed
    b>Intermediate filaments (IFs) constitute a major structural element of animal cells. They build two distinct systems, one in the nucleus and one in the cytoplasm...
  36. Kirmse R, Portet S, Mücke N, Aebi U, Herrmann H, Langowski J. A quantitative kinetic model for the in vitro assembly of intermediate filaments from tetrameric vimentin. J Biol Chem. 2007;282:18563-72 pubmed
  37. Potokar M, Stenovec M, Gabrijel M, Li L, Kreft M, Grilc S, et al. Intermediate filaments attenuate stimulation-dependent mobility of endosomes/lysosomes in astrocytes. Glia. 2010;58:1208-19 pubmed publisher
    ..We propose that upregulation of IFs in pathologic states may alter the function of astrocytes by deregulating vesicle trafficking...
  38. Toth C, Shim S, Wang J, Jiang Y, Neumayer G, Belzil C, et al. Ndel1 promotes axon regeneration via intermediate filaments. PLoS ONE. 2008;3:e2014 pubmed publisher
    ..Thus, Ndel1 promotes axonal regeneration; activating this endogenous repair mechanism may enhance neuroregeneration during acute and chronic axonal degeneration...
  39. de Pablo Y, Nilsson M, Pekna M, Pekny M. Intermediate filaments are important for astrocyte response to oxidative stress induced by oxygen-glucose deprivation and reperfusion. Histochem Cell Biol. 2013;140:81-91 pubmed publisher
    ..Astrocytes in mice devoid of GFAP and vimentin (GFAP (-/-) Vim (-/-)) do not form cytoplasmic intermediate filaments. GFAP (-/-) Vim (-/-) mice develop larger infarcts after ischemic stroke (Li et al...
  40. Heimfarth L, Loureiro S, Reis K, de Lima B, Zamboni F, Lacerda S, et al. Diphenyl ditelluride induces hypophosphorylation of intermediate filaments through modulation of DARPP-32-dependent pathways in cerebral cortex of young rats. Arch Toxicol. 2012;86:217-30 pubmed publisher
  41. Chen J, Cheng X, Merched Sauvage M, Caulin C, Roop D, Koch P. An unexpected role for keratin 10 end domains in susceptibility to skin cancer. J Cell Sci. 2006;119:5067-76 pubmed
    ..Our results support the notion that intermediate filaments, in addition to their function as cytoskeletal components, affect tumor susceptibility of epithelial cells.
  42. Harada M, Strnad P, Resurreccion E, Ku N, Omary M. Keratin 18 overexpression but not phosphorylation or filament organization blocks mouse Mallory body formation. Hepatology. 2007;45:88-96 pubmed
    ..DDC feeding induced MBs in nontransgenic livers, but MBs were rarely seen in any of the K18 transgenic mice. Wild-type K18 overexpression protected mice from DDC-induced liver injury...
  43. Rezniczek G, Konieczny P, Nikolic B, Reipert S, Schneller D, Abrahamsberg C, et al. Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle fibers through multiple interactions with beta-dystroglycan. J Cell Biol. 2007;176:965-77 pubmed
    ..It also explains the plectin phenotype observed in dystrophic skeletal muscle of mdx mice and Duchenne muscular dystrophy patients...
  44. Schopferer M, Bär H, Hochstein B, Sharma S, Mücke N, Herrmann H, et al. Desmin and vimentin intermediate filament networks: their viscoelastic properties investigated by mechanical rheometry. J Mol Biol. 2009;388:133-43 pubmed publisher
    ..These findings indicate that both IFs exhibit distinct mechanical properties that are adapted to their respective cellular surroundings [i.e., myocytes (desmin) and fibroblasts (vimentin)]...
  45. Kim S, Coulombe P. Intermediate filament scaffolds fulfill mechanical, organizational, and signaling functions in the cytoplasm. Genes Dev. 2007;21:1581-97 pubmed
    b>Intermediate filaments (IFs) are cytoskeletal polymers whose protein constituents are encoded by a large family of differentially expressed genes...
  46. Spazierer D, Raberger J, Gross K, Fuchs P, Wiche G. Stress-induced recruitment of epiplakin to keratin networks increases their resistance to hyperphosphorylation-induced disruption. J Cell Sci. 2008;121:825-33 pubmed publisher
    ..Our data suggest that epiplakin plays a role in keratin filament reorganization in response to stress, probably by protecting keratin filaments against disruption in a chaperone-like fashion...
  47. Potokar M, Kreft M, Li L, Daniel Andersson J, Pangrsic T, Chowdhury H, et al. Cytoskeleton and vesicle mobility in astrocytes. Traffic. 2007;8:12-20 pubmed
    ..peptide and monitored their mobility in rat astrocytes with depolymerized microtubules, actin, and intermediate filaments and in mouse astrocytes deficient in the intermediate filament proteins glial fibrillary acidic protein ..
  48. Park J, Kim H, Lee S, Lee Y, Choi U, Kang J, et al. Differential expression of intermediate filaments in the process of developing hepatic steatosis. Proteomics. 2011;11:2777-89 pubmed publisher
    ..Our results suggest that vim, like CK-18, may be a useful marker for predicting obesity and liver disease...
  49. Burgstaller G, Gregor M, Winter L, Wiche G. Keeping the vimentin network under control: cell-matrix adhesion-associated plectin 1f affects cell shape and polarity of fibroblasts. Mol Biol Cell. 2010;21:3362-75 pubmed publisher
    ..their derivative fibronectin fibril-aligned fibrillar adhesions (FbAs) serve as docking sites for vimentin intermediate filaments (IFs) in a plectin isoform 1f (P1f)-dependent manner...
  50. Abe S, Rhee S, Osonoi M, Nakamura T, Cho B, Murakami G, et al. Expression of intermediate filaments at muscle insertions in human fetuses. J Anat. 2010;217:167-73 pubmed publisher
    Desmin and vimentin are intermediate filaments that play crucial roles in the maturation, maintenance and recovery of muscle fibers and mesenchymal cells...
  51. Leccia E, Batonnet Pichon S, Tarze A, Bailleux V, Doucet J, Pelloux M, et al. Cyclic stretch reveals a mechanical role for intermediate filaments in a desminopathic cell model. Phys Biol. 2013;10:016001 pubmed publisher
    ..have been inferred from experiments which investigate the roles of actin and microtubules ignoring the intermediate filaments (IFs) contribution...
  52. Capetanaki Y, Bloch R, Kouloumenta A, Mavroidis M, Psarras S. Muscle intermediate filaments and their links to membranes and membranous organelles. Exp Cell Res. 2007;313:2063-76 pubmed
    b>Intermediate filaments (IFs) play a key role in the integration of structure and function of striated muscle, primarily by mediating mechanochemical links between the contractile apparatus and mitochondria, myonuclei, the sarcolemma and ..
  53. Deng M, Mohanan S, Polyak E, Chacko S. Caldesmon is necessary for maintaining the actin and intermediate filaments in cultured bladder smooth muscle cells. Cell Motil Cytoskeleton. 2007;64:951-65 pubmed
    ..This finding raises the possibility that the cytoskeletal structure in smooth muscle is affected when CaD expression is altered, as in smooth muscle de-differentiation and hypertrophy seen in certain pathological conditions...