stress fibers

Summary

Summary: Bundles of actin filaments (ACTIN CYTOSKELETON) and myosin-II that span across the cell attaching to the cell membrane at FOCAL ADHESIONS and to the network of INTERMEDIATE FILAMENTS that surrounds the nucleus.

Top Publications

  1. Breuiller Fouche M, Dubois O, Sediki M, Garcia Verdugo I, Palaniyar N, Tanfin Z, et al. Secreted surfactant protein A from fetal membranes induces stress fibers in cultured human myometrial cells. Am J Physiol Endocrinol Metab. 2010;298:E1188-97 pubmed publisher
    ..These data indicate that SP-A released by human fetal membranes is able to exert a paracrine regulation of F-actin filament organization in myometrial cells...
  2. Hoffman L, Jensen C, Chaturvedi A, Yoshigi M, Beckerle M. Stretch-induced actin remodeling requires targeting of zyxin to stress fibers and recruitment of actin regulators. Mol Biol Cell. 2012;23:1846-59 pubmed publisher
    Reinforcement of actin stress fibers in response to mechanical stimulation depends on a posttranslational mechanism that requires the LIM protein zyxin...
  3. Vallenius T, Vaahtomeri K, Kovac B, Osiceanu A, Viljanen M, Makela T. An association between NUAK2 and MRIP reveals a novel mechanism for regulation of actin stress fibers. J Cell Sci. 2011;124:384-93 pubmed publisher
    ..Association of NUAK2 with MRIP increases MLC phosphorylation and promotes formation of stress fibers. This activity does not require the kinase activity of NUAK2 but is dependent on both MRIP and MYPT1, ..
  4. Kobayashi T, Sokabe M. Sensing substrate rigidity by mechanosensitive ion channels with stress fibers and focal adhesions. Curr Opin Cell Biol. 2010;22:669-76 pubmed publisher
    ..To sense substrate rigidity, cells apply traction forces to cell-substrate adhesions via actin stress fibers (SFs) and measure mechanical responses of the substrate...
  5. Hsu H, Lee C, Kaunas R. A dynamic stochastic model of frequency-dependent stress fiber alignment induced by cyclic stretch. PLoS ONE. 2009;4:e4853 pubmed publisher
    Actin stress fibers (SFs) are mechanosensitive structural elements that respond to forces to affect cell morphology, migration, signal transduction and cell function...
  6. Deguchi S, Sato M. Biomechanical properties of actin stress fibers of non-motile cells. Biorheology. 2009;46:93-105 pubmed publisher
    The structure of stress fibers, contractile actin bundles, differs between motile and non-motile cells, though the same term "stress fiber" is used to refer to the structurally as well as functionally different actin bundles...
  7. Tojkander S, Gateva G, Schevzov G, Hotulainen P, Naumanen P, Martin C, et al. A molecular pathway for myosin II recruitment to stress fibers. Curr Biol. 2011;21:539-50 pubmed publisher
    ..In contrast, the mechanisms by which contractile actomyosin arrays such as stress fibers are generated in cells, and how myosin II is specifically recruited to these structures, are not known.
  8. Pattabiraman P, Rao P. Mechanistic basis of Rho GTPase-induced extracellular matrix synthesis in trabecular meshwork cells. Am J Physiol Cell Physiol. 2010;298:C749-63 pubmed publisher
    ..laminin, alpha-smooth muscle actin (alpha-SMA) levels, and matrix assembly in association with increased actin stress fibers and myosin light-chain phosphorylation...
  9. Alves Silva J, Hahn I, Huber O, Mende M, Reissaus A, Prokop A. Prominent actin fiber arrays in Drosophila tendon cells represent architectural elements different from stress fibers. Mol Biol Cell. 2008;19:4287-97 pubmed publisher
    ..The F-actin arrays in tendon cells seem to represent a new kind of actin structure, clearly distinct from stress fibers. They are highly resistant to F-actin-destabilizing drugs, to the application of myosin blockers, and to loss ..

More Information

Publications62

  1. Hwang Y, Barakat A. Dynamics of mechanical signal transmission through prestressed stress fibers. PLoS ONE. 2012;7:e35343 pubmed publisher
    ..We develop a model of mechanical signal transmission through prestressed viscoelastic actin stress fibers that directly connect the cell surface to the nucleus...
  2. Wei Z, Deshpande V, McMeeking R, Evans A. Analysis and interpretation of stress fiber organization in cells subject to cyclic stretch. J Biomech Eng. 2008;130:031009 pubmed publisher
    ..Uniaxial cyclic stretching of cells causes stress fibers to align perpendicular to the stretch direction, with degree of alignment dependent on the stretch strain ..
  3. Chapin L, Blankman E, Smith M, Shiu Y, Beckerle M. Lateral communication between stress fiber sarcomeres facilitates a local remodeling response. Biophys J. 2012;103:2082-92 pubmed publisher
    Actin stress fibers (SFs) are load-bearing and mechanosensitive structures. To our knowledge, the mechanisms that enable SFs to sense and respond to strain have not been fully defined...
  4. Fan L, Pellegrin S, Scott A, Mellor H. The small GTPase Rif is an alternative trigger for the formation of actin stress fibers in epithelial cells. J Cell Sci. 2010;123:1247-52 pubmed publisher
    Actin stress fibers are fundamental components of the actin cytoskeleton that produce contractile force in non-muscle cells...
  5. Xie Y, Tan E, Wee S, Manser E, Lim L, Koh C. Functional interactions between phosphatase POPX2 and mDia modulate RhoA pathways. J Cell Sci. 2008;121:514-21 pubmed publisher
    ..We propose that the interaction between mDia1 and POPX2 (PPM1F) serves to regulate both the actin cytoskeleton and SRF-mediated transcription, and to link the CDC42/RAC1 pathways with those of RhoA...
  6. Hayakawa K, Tatsumi H, Sokabe M. Actin stress fibers transmit and focus force to activate mechanosensitive channels. J Cell Sci. 2008;121:496-503 pubmed publisher
    ..This study provides the first direct evidence that the cytoskeleton works as a force-transmitting and force-focusing molecular device to activate MS channels in eukaryotic cells...
  7. Lu L, Feng Y, Hucker W, Oswald S, Longmore G, Yin F. Actin stress fiber pre-extension in human aortic endothelial cells. Cell Motil Cytoskeleton. 2008;65:281-94 pubmed publisher
    Actin stress fibers (SFs) enable cells to sense and respond to mechanical stimuli and affect adhesion, motility and apoptosis...
  8. Simovitch M, Sason H, Cohen S, Zahavi E, Melamed Book N, Weiss A, et al. EspM inhibits pedestal formation by enterohaemorrhagic Escherichia coli and enteropathogenic E. coli and disrupts the architecture of a polarized epithelial monolayer. Cell Microbiol. 2010;12:489-505 pubmed publisher
    ..Taken together, our results show that the EspM effectors inhibit pedestal formation and induce tight junction mislocalization as well as dramatic changes in the architecture of the polarized monolayer...
  9. Hale C, Sun S, Wirtz D. Resolving the role of actoymyosin contractility in cell microrheology. PLoS ONE. 2009;4:e7054 pubmed publisher
    ..lodged in the cytoplasm, reveal the biphasic mechanical architecture of adherent cells-stiff contractile stress fibers interdigitating in a network at the cell cortex and a soft actin meshwork in the body of the cell, validate ..
  10. Lee C, Haase C, Deguchi S, Kaunas R. Cyclic stretch-induced stress fiber dynamics - dependence on strain rate, Rho-kinase and MLCK. Biochem Biophys Res Commun. 2010;401:344-9 pubmed publisher
    ..Instead, these distal ends were often used to assemble new stress fibers oriented progressively further away from the direction of stretch...
  11. Susila A, Chan H, Loh A, Phang H, Wong E, Tergaonkar V, et al. The POPX2 phosphatase regulates cancer cell motility and invasiveness. Cell Cycle. 2010;9:179-87 pubmed
    ..We show that silencing of POPX2 reduces the amount of stress fibers and focal adhesions in both cells lines...
  12. Patla I, Volberg T, Elad N, Hirschfeld Warneken V, Grashoff C, Fassler R, et al. Dissecting the molecular architecture of integrin adhesion sites by cryo-electron tomography. Nat Cell Biol. 2010;12:909-15 pubmed publisher
    ..Our findings clarify the internal architecture of focal adhesions at molecular resolution, and provide insights into their scaffolding and mechanosensory functions...
  13. Katoh K, Kano Y, Ookawara S. Role of stress fibers and focal adhesions as a mediator for mechano-signal transduction in endothelial cells in situ. Vasc Health Risk Manag. 2008;4:1273-82 pubmed
    ..The findings of a recent study suggest that stress fibers and its associated focal adhesions play roles in mechano-signal transduction mechanism...
  14. Aratyn Schaus Y, Oakes P, Gardel M. Dynamic and structural signatures of lamellar actomyosin force generation. Mol Biol Cell. 2011;22:1330-9 pubmed publisher
    ..Diverse lamellar actin organizations ranging from contractile lamellar networks to stress fibers are observed in adherent cells...
  15. Faust U, Hampe N, Rubner W, Kirchgessner N, Safran S, Hoffmann B, et al. Cyclic stress at mHz frequencies aligns fibroblasts in direction of zero strain. PLoS ONE. 2011;6:e28963 pubmed publisher
    ..Cyclic stretch increases the number of stress fibers and the coupling to adhesions...
  16. Chang C, Kumar S. Vinculin tension distributions of individual stress fibers within cell-matrix adhesions. J Cell Sci. 2013;126:3021-30 pubmed publisher
    Actomyosin stress fibers (SFs) enable cells to exert traction on planar extracellular matrices (ECMs) by tensing focal adhesions (FAs) at the cell-ECM interface...
  17. Takeya R, Taniguchi K, Narumiya S, Sumimoto H. The mammalian formin FHOD1 is activated through phosphorylation by ROCK and mediates thrombin-induced stress fibre formation in endothelial cells. EMBO J. 2008;27:618-28 pubmed publisher
  18. Wada K, Itoga K, Okano T, Yonemura S, Sasaki H. Hippo pathway regulation by cell morphology and stress fibers. Development. 2011;138:3907-14 pubmed publisher
    ..b>Stress fibers increased in response to expanded cell areas, and F-actin regulated Yap downstream of cell morphology...
  19. Nagayama K, Yahiro Y, Matsumoto T. Stress fibers stabilize the position of intranuclear DNA through mechanical connection with the nucleus in vascular smooth muscle cells. FEBS Lett. 2011;585:3992-7 pubmed publisher
    Actin stress fibers (SFs) running across the top surface of the nucleus in vascular smooth muscle cells were dissected using laser nano-dissection technique to release its pretension, and the dynamic behavior of SFs, nucleus, and ..
  20. Wang L, Ellis M, Gomez J, Eisner W, Fennell W, Howell D, et al. Mechanisms of the proteinuria induced by Rho GTPases. Kidney Int. 2012;81:1075-85 pubmed publisher
    ..In contrast, the dominant-negative N19 Rho caused a loss of podocyte stress fibers, did not alter the expression of either nephrin or Rho A, and did not cause podocyte apoptosis...
  21. Burridge K, Wittchen E. The tension mounts: stress fibers as force-generating mechanotransducers. J Cell Biol. 2013;200:9-19 pubmed publisher
    b>Stress fibers (SFs) are often the most prominent cytoskeletal structures in cells growing in tissue culture...
  22. Smith M, Blankman E, Gardel M, Luettjohann L, Waterman C, Beckerle M. A zyxin-mediated mechanism for actin stress fiber maintenance and repair. Dev Cell. 2010;19:365-76 pubmed publisher
    ..By imaging live cells expressing fluorescently tagged cytoskeletal proteins, we observed that actin stress fibers undergo local, acute, force-induced elongation and thinning events that compromise their stress transmission ..
  23. Babakov V, Petukhova O, Turoverova L, Kropacheva I, Tentler D, Bolshakova A, et al. RelA/NF-kappaB transcription factor associates with alpha-actinin-4. Exp Cell Res. 2008;314:1030-8 pubmed publisher
    ..NF-kappaB and alpha-actinin-4 co-localized along actin stress fibers and in membrane lamellae in A431 cells...
  24. Katoh K, Kano Y, Noda Y. Rho-associated kinase-dependent contraction of stress fibres and the organization of focal adhesions. J R Soc Interface. 2011;8:305-11 pubmed publisher
    ..Activated Rho-kinase is also involved in the organization of newly formed stress fibres and focal adhesions in living cells...
  25. Hsu H, Lee C, Locke A, Vanderzyl S, Kaunas R. Stretch-induced stress fiber remodeling and the activations of JNK and ERK depend on mechanical strain rate, but not FAK. PLoS ONE. 2010;5:e12470 pubmed publisher
    ..Cells sense and dynamically respond to stretching of the matrix by reorienting their actin stress fibers and by activating intracellular signaling proteins, including focal adhesion kinase (FAK) and the mitogen-..
  26. Millan J, Cain R, Reglero Real N, Bigarella C, Marcos Ramiro B, Fernández Martín L, et al. Adherens junctions connect stress fibres between adjacent endothelial cells. BMC Biol. 2010;8:11 pubmed publisher
    ..However, in the endothelium, cell-cell junctions are highly dynamic and morphologically heterogeneous...
  27. Lefranc F, Sauvage S, Van Goietsenoven G, Megalizzi V, Lamoral Theys D, Debeir O, et al. Narciclasine, a plant growth modulator, activates Rho and stress fibers in glioblastoma cells. Mol Cancer Ther. 2009;8:1739-50 pubmed publisher
    ..Our results show for the first time that narciclasine, a plant growth modulator, activates Rho and stress fibers in glioblastoma multiforme cells and significantly increases the survival of human glioblastoma multiforme ..
  28. Acharya P, Majumdar S, Jacob M, Hayden J, Mrass P, Weninger W, et al. Fibroblast migration is mediated by CD44-dependent TGF beta activation. J Cell Sci. 2008;121:1393-402 pubmed publisher
    ..CD44KO fibroblasts exhibited fewer stress fibers and focal adhesion complexes, and their migration was characterized by increased velocity but loss of ..
  29. Kaunas R, Hsu H. A kinematic model of stretch-induced stress fiber turnover and reorientation. J Theor Biol. 2009;257:320-30 pubmed publisher
    A kinetic model based on constrained mixture theory was developed to describe the reorganization of actin stress fibers in adherent cells in response to diverse patterns of mechanical stretch...
  30. De R, Safran S. Dynamical theory of active cellular response to external stress. Phys Rev E Stat Nonlin Soft Matter Phys. 2008;78:031923 pubmed
    ..forces that arise from the deformation of the matrix as well as forces due to the internal regulation of the stress fibers and focal adhesions of the cell...
  31. Bogatcheva N, Verin A. The role of cytoskeleton in the regulation of vascular endothelial barrier function. Microvasc Res. 2008;76:202-7 pubmed publisher
    ..This review will analyze the cytoskeletal elements, which reorganization affects endothelial permeability, and emphasize signaling mechanisms with barrier-protective or barrier-disruptive potential...
  32. Vallenius T. Actin stress fibre subtypes in mesenchymal-migrating cells. Open Biol. 2013;3:130001 pubmed publisher
    ..This present review focuses on the current view of the molecular composition of actin stress fibre subtypes and how these fibre subtypes regulate mesenchymal cell migration...
  33. De R, Zemel A, Safran S. Theoretical concepts and models of cellular mechanosensing. Methods Cell Biol. 2010;98:143-75 pubmed publisher
  34. Kakinuma N, Roy B, Zhu Y, Wang Y, Kiyama R. Kank regulates RhoA-dependent formation of actin stress fibers and cell migration via 14-3-3 in PI3K-Akt signaling. J Cell Biol. 2008;181:537-49 pubmed publisher
    ..In NIH3T3 cells expressing Kank, the amount of actin stress fibers is reduced, and the coexpression of 14-3-3 disrupted this effect...
  35. Kovac B, Teo J, Makela T, Vallenius T. Assembly of non-contractile dorsal stress fibers requires ?-actinin-1 and Rac1 in migrating and spreading cells. J Cell Sci. 2013;126:263-73 pubmed publisher
    Cell migration and spreading is driven by actin polymerization and actin stress fibers. Actin stress fibers are considered to contain ?-actinin crosslinkers and nonmuscle myosin II motors...
  36. Miyazaki K, Ohno K, Tamura N, Sasaki T, Sato K. CLP36 and RIL recruit ?-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts. Exp Cell Res. 2012;318:1716-25 pubmed publisher
    CLP36 is a member of the ALP/Enigma protein family and has been shown to be localized to stress fibers in various cells...
  37. Tojkander S, Gateva G, Lappalainen P. Actin stress fibers--assembly, dynamics and biological roles. J Cell Sci. 2012;125:1855-64 pubmed publisher
    ..b>Stress fibers are contractile actomyosin bundles found in many cultured non-muscle cells, where they have a central role in ..
  38. Nagayama K, Kimura Y, Makino N, Matsumoto T. Strain waveform dependence of stress fiber reorientation in cyclically stretched osteoblastic cells: effects of viscoelastic compression of stress fibers. Am J Physiol Cell Physiol. 2012;302:C1469-78 pubmed publisher
    Actin stress fibers (SFs) of cells cultured on cyclically stretched substrate tend to reorient in the direction in which a normal strain of substrate becomes zero. However, little is known about the mechanism of this reorientation...
  39. Oakes P, Beckham Y, Stricker J, Gardel M. Tension is required but not sufficient for focal adhesion maturation without a stress fiber template. J Cell Biol. 2012;196:363-74 pubmed publisher
    ..This work identifies the essential role of lamellar actin architecture in adhesion maturation...
  40. Lu L, Oswald S, Ngu H, Yin F. Mechanical properties of actin stress fibers in living cells. Biophys J. 2008;95:6060-71 pubmed publisher
    Actin stress fibers (SFs) play an important role in many cellular functions, including morphological stability, adhesion, and motility...
  41. Rosker C, Salvarani N, Schmutz S, Grand T, Rohr S. Abolishing myofibroblast arrhythmogeneicity by pharmacological ablation of ?-smooth muscle actin containing stress fibers. Circ Res. 2011;109:1120-31 pubmed publisher
    ..So far, it is not known whether ?-smooth muscle actin (?-SMA) containing stress fibers, the cytoskeletal components that set myofibroblasts apart from resident fibroblasts, are essential for ..
  42. Desforges B, Savarin P, Bounedjah O, Delga S, Hamon L, Curmi P, et al. Gap junctions favor normal rat kidney epithelial cell adaptation to chronic hypertonicity. Am J Physiol Cell Physiol. 2011;301:C705-16 pubmed publisher
    ..In addition to this, we suggest that cancer cells that do not communicate via gap junctions poorly cope with hypertonic environments thus explaining the rare occurrence of cancer coming from the kidney medulla...
  43. Leung W, Ching A, Wong N. Phosphorylation of Caldesmon by PFTAIRE1 kinase promotes actin binding and formation of stress fibers. Mol Cell Biochem. 2011;350:201-6 pubmed publisher
    ..Without the binding of CaD to actin, disappearance of actin stress fibers was also evident in PFTK1-abrogated cells...
  44. Lai F, Szczodrak M, Oelkers J, Ladwein M, Acconcia F, Benesch S, et al. Cortactin promotes migration and platelet-derived growth factor-induced actin reorganization by signaling to Rho-GTPases. Mol Biol Cell. 2009;20:3209-23 pubmed publisher
    ..Furthermore, we propose that cortactin promotes cell migration indirectly, through contributing to activation of selected Rho-GTPases. ..
  45. Kim M, Kim S, Kim Y, Jin E, Sonn J. Inhibition of RhoA but not ROCK induces chondrogenesis of chick limb mesenchymal cells. Biochem Biophys Res Commun. 2012;418:500-5 pubmed publisher
    ..CT04, induced chondrogenesis of undifferentiated mesenchymal single cells following dissolution of actin stress fibers. Inhibition of RhoA activity by CT04 was confirmed by pull down assay using the Rho-GTP binding domain of ..
  46. Goeckeler Z, Bridgman P, Wysolmerski R. Nonmuscle myosin II is responsible for maintaining endothelial cell basal tone and stress fiber integrity. Am J Physiol Cell Physiol. 2008;295:C994-1006 pubmed publisher
    ..incorporation and association with polymerizing or reorganized actin filaments leading to the disappearance of stress fibers. Thus the inhibitory effects of blebbistatin on basal tone and induced tension are consistent with a ..
  47. Naumanen P, Lappalainen P, Hotulainen P. Mechanisms of actin stress fibre assembly. J Microsc. 2008;231:446-54 pubmed publisher
    ..In this review, we discuss the definition of stress fibres, organization of actin filaments and other components within these contractile structures, and the mechanisms of stress fibre assembly...
  48. Kong D, Ji B, Dai L. Stability of adhesion clusters and cell reorientation under lateral cyclic tension. Biophys J. 2008;95:4034-44 pubmed publisher
    ..Furthermore, we find that the stiffness and relaxation time of stress fibers play important roles in the stability of the adhesion cluster...
  49. Sohail M, Hashmi A, Hakim W, Watanabe A, Zipprich A, Groszmann R, et al. Adenosine induces loss of actin stress fibers and inhibits contraction in hepatic stellate cells via Rho inhibition. Hepatology. 2009;49:185-94 pubmed publisher
    ..pathway is activated in differentiated hepatic stellate cells (HSCs) and is necessary for assembly of actin stress fibers, contractility, and chemotaxis...
  50. Colombelli J, Besser A, Kress H, Reynaud E, Girard P, Caussinus E, et al. Mechanosensing in actin stress fibers revealed by a close correlation between force and protein localization. J Cell Sci. 2009;122:1665-79 pubmed publisher
    ..cytoskeleton laser nanosurgery in cultured epithelial cells and fibroblasts, we show that the retraction of stress fibers (SFs) is restricted to the proximity of the cut and that new adhesions form at the retracting end...
  51. Schönichen A, Mannherz H, Behrmann E, Mazur A, Kuhn S, Silvan U, et al. FHOD1 is a combined actin filament capping and bundling factor that selectively associates with actin arcs and stress fibers. J Cell Sci. 2013;126:1891-901 pubmed publisher
    ..At the base of lamellipodia, FHOD1 is enriched in nascent, bundled actin arcs as well as in more mature stress fibers. This function requires actin-binding domains located N-terminally to the canonical FH1-FH2 element...
  52. Gudermann T, Steinritz D. STIMulating stress fibers in endothelial cells. Sci Signal. 2013;6:pe8 pubmed publisher
    ..This SOCE-independent role of STIM1 highlights a surprising new aspect of this multifaceted cellular regulator...
  53. Arbeloa A, Bulgin R, MacKenzie G, Shaw R, Pallen M, Crepin V, et al. Subversion of actin dynamics by EspM effectors of attaching and effacing bacterial pathogens. Cell Microbiol. 2008;10:1429-41 pubmed publisher
    ..These results suggest that the EspM effectors modulate actin dynamics by activating the RhoA signalling pathway...