cytoplasmic structures

Summary

Summary: Components of the cytoplasm excluding the CYTOSOL.

Top Publications

  1. Jakymiw A, Pauley K, Li S, Ikeda K, Lian S, Eystathioy T, et al. The role of GW/P-bodies in RNA processing and silencing. J Cell Sci. 2007;120:1317-23 pubmed
    ..Here, we propose a working model for GW body assembly in terms of its relationship to RNA interference. In this process, one or more heteromeric protein complexes accumulate in successive steps into larger ribonucleoprotein structures...
  2. Pilkington G, Parker R. Pat1 contains distinct functional domains that promote P-body assembly and activation of decapping. Mol Cell Biol. 2008;28:1298-312 pubmed
    ..These results indicate that Pat1 is an RNA binding protein and a multidomain protein that functions at multiple stages in the process of translation repression and mRNA decapping...
  3. Sheth U, Parker R. Targeting of aberrant mRNAs to cytoplasmic processing bodies. Cell. 2006;125:1095-109 pubmed
    ..Moreover, Upf1p can target normal mRNAs to P-bodies but not promote their degradation. These observations lead us to propose a new model for NMD wherein two successive steps are used to distinguish normal and aberrant mRNAs...
  4. Chu C, Rana T. Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54. PLoS Biol. 2006;4:e210 pubmed
    ..These studies also suggest that translation suppression by miRISC does not require P-body structures, and location of miRISC to P-bodies is the consequence of translation repression...
  5. Beckham C, Parker R. P bodies, stress granules, and viral life cycles. Cell Host Microbe. 2008;3:206-12 pubmed publisher
    ..Thus, an important interplay among P bodies, stress granules, and viral life cycles is beginning to emerge...
  6. Jakymiw A, Lian S, Eystathioy T, Li S, Satoh M, Hamel J, et al. Disruption of GW bodies impairs mammalian RNA interference. Nat Cell Biol. 2005;7:1267-74 pubmed
    ..Our data support a model in which GW182 and/or the microenvironment of the cytoplasmic GWBs contribute to the RNA-induced silencing complex and to RNA silencing...
  7. Carter V, Shimizu S, Arai M, Dessens J. PbSR is synthesized in macrogametocytes and involved in formation of the malaria crystalloids. Mol Microbiol. 2008;68:1560-9 pubmed publisher
    ..These findings are the first to identify a parasite protein involved with the crystalloid organelle, and suggest a novel protein-trafficking mechanism to deliver PbSR to the oocysts...
  8. Anderson P. A Place for RNAi. Dev Cell. 2005;9:311-2 pubmed
    ..In a recent issue of Molecular Cell, Ding and coworkers described an argonaute-interacting protein that appears to promote the assembly of P bodies in C. elegans (Ding et al., 2005)...
  9. Pillai R, Bhattacharyya S, Filipowicz W. Repression of protein synthesis by miRNAs: how many mechanisms?. Trends Cell Biol. 2007;17:118-26 pubmed
    ..Recent data support different microRNA mechanisms and a role for cytoplasmic processing bodies in the degradation and storage of mRNAs targeted by microRNA regulators...

More Information

Publications62

  1. Rossi J. RNAi and the P-body connection. Nat Cell Biol. 2005;7:643-4 pubmed
  2. Carbonaro M, O Brate A, Giannakakou P. Microtubule disruption targets HIF-1alpha mRNA to cytoplasmic P-bodies for translational repression. J Cell Biol. 2011;192:83-99 pubmed publisher
    ..Collectively, we provide evidence for a new mechanism of microtubule-dependent HIF-1? translation with important implications for cell biology...
  3. Necchi V, Balduini A, Noris P, Barozzi S, Sommi P, di Buduo C, et al. Ubiquitin/proteasome-rich particulate cytoplasmic structures (PaCSs) in the platelets and megakaryocytes of ANKRD26-related thrombo-cytopenia. Thromb Haemost. 2013;109:263-71 pubmed publisher
    ..PaCSs are novel particulate cytoplasmic structures with selective immunoreactivity for polyubiquitinated proteins and proteasome that have been detected in a ..
  4. Sheth U, Parker R. Decapping and decay of messenger RNA occur in cytoplasmic processing bodies. Science. 2003;300:805-8 pubmed
    ..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
  5. Yang Z, Jakymiw A, Wood M, Eystathioy T, Rubin R, Fritzler M, et al. GW182 is critical for the stability of GW bodies expressed during the cell cycle and cell proliferation. J Cell Sci. 2004;117:5567-78 pubmed
  6. Pauley K, Satoh M, Pauley B, Dominguez Gutierrez P, Wallet S, Holliday L, et al. Formation of GW/P bodies as marker for microRNA-mediated regulation of innate immune signaling in THP-1 cells. Immunol Cell Biol. 2010;88:205-12 pubmed publisher
    ..These data show that the increase in size and number of GWB may serve as a biomarker for miRNA-mediated gene regulation, and miR-146a has a significant role in the regulation of LPS-induced cytokine production in THP-1 cells...
  7. Liu J, Rivas F, Wohlschlegel J, Yates J, Parker R, Hannon G. A role for the P-body component GW182 in microRNA function. Nat Cell Biol. 2005;7:1261-6 pubmed
    ..Thus, our results support a functional link between cytoplasmic P-bodies and the ability of a microRNA to repress expression of a target mRNA...
  8. Avesani F, Romanelli M, Turci M, Di Gennaro G, Sampaio C, Bidoia C, et al. Association of HTLV Tax proteins with TAK1-binding protein 2 and RelA in calreticulin-containing cytoplasmic structures participates in Tax-mediated NF-?B activation. Virology. 2010;408:39-48 pubmed publisher
    ..These two NF-?B factors colocalized with Tax proteins in dotted cytoplasmic structures targeted by calreticulin, a multi-process calcium-buffering chaperone...
  9. Fillman C, Lykke Andersen J. RNA decapping inside and outside of processing bodies. Curr Opin Cell Biol. 2005;17:326-31 pubmed
    ..Recent evidence suggests that the processing bodies may constitute specialized cellular compartments of mRNA turnover, which suggests that mRNA and protein localization may be integral to mRNA decay...
  10. Bhattacharyya S, Habermacher R, Martine U, Closs E, Filipowicz W. Relief of microRNA-mediated translational repression in human cells subjected to stress. Cell. 2006;125:1111-24 pubmed
    ..We propose that proteins interacting with the 3'UTR will generally act as modifiers altering the potential of miRNAs to repress gene expression...
  11. Noble S, Allen B, Goh L, Nordick K, Evans T. Maternal mRNAs are regulated by diverse P body-related mRNP granules during early Caenorhabditis elegans development. J Cell Biol. 2008;182:559-72 pubmed publisher
    ..Therefore, a system of diverse and regulated RNP granules elicits stage-specific functions that ensure proper mRNA control during early development...
  12. Lian S, Fritzler M, Katz J, Hamazaki T, Terada N, Satoh M, et al. Small interfering RNA-mediated silencing induces target-dependent assembly of GW/P bodies. Mol Biol Cell. 2007;18:3375-87 pubmed
    ..We propose that RNAi is a key regulatory mechanism for the assembly of GWB, and in some cases, GWB may serve as markers for RNAi in mammalian cells...
  13. Eulalio A, Behm Ansmant I, Schweizer D, Izaurralde E. P-body formation is a consequence, not the cause, of RNA-mediated gene silencing. Mol Cell Biol. 2007;27:3970-81 pubmed
    ..Thus, even though P-body components play crucial roles in mRNA silencing and decay, aggregation into P bodies is not required for function but is instead a consequence of their activity...
  14. Bhattacharyya S, Habermacher R, Martine U, Closs E, Filipowicz W. Stress-induced reversal of microRNA repression and mRNA P-body localization in human cells. Cold Spring Harb Symp Quant Biol. 2006;71:513-21 pubmed
    ..We propose that proteins interacting with the 3'UTR will generally act as modifiers altering the potential of miRNAs to repress gene expression...
  15. Eulalio A, Behm Ansmant I, Izaurralde E. P bodies: at the crossroads of post-transcriptional pathways. Nat Rev Mol Cell Biol. 2007;8:9-22 pubmed
  16. Zheng D, Ezzeddine N, Chen C, Zhu W, He X, Shyu A. Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells. J Cell Biol. 2008;182:89-101 pubmed publisher
    ..These results support a dynamic interplay among deadenylation, mRNP remodeling, and P-body formation in selective decay of mammalian mRNA...
  17. Parker R, Sheth U. P bodies and the control of mRNA translation and degradation. Mol Cell. 2007;25:635-46 pubmed
    ..Moreover, some mRNA-specific regulatory factors, including miRNAs and RISC, appear to repress translation and promote decay by recruiting P body components to individual mRNAs...
  18. Labernadie A, Thibault C, Vieu C, Maridonneau Parini I, Charriere G. Dynamics of podosome stiffness revealed by atomic force microscopy. Proc Natl Acad Sci U S A. 2010;107:21016-21 pubmed publisher
    ..Such an approach opens up perspectives to better understand the mechanical functionality of podosomes under physiological and pathological contexts...
  19. Necchi V, Minelli A, Sommi P, Vitali A, Caruso R, Longoni D, et al. Ubiquitin-proteasome-rich cytoplasmic structures in neutrophils of patients with Shwachman-Diamond syndrome. Haematologica. 2012;97:1057-63 pubmed publisher
    ..Recently, novel particulate cytoplasmic structures, rich in ubiquitinated and proteasomal proteins, have been detected in epithelial cells and neutrophils ..
  20. Pauley K, Eystathioy T, Jakymiw A, Hamel J, Fritzler M, Chan E. Formation of GW bodies is a consequence of microRNA genesis. EMBO Rep. 2006;7:904-10 pubmed
    ..Taken together, these studies show that miRNAs are crucial components in GWB formation...
  21. Liu J, Valencia Sanchez M, Hannon G, Parker R. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat Cell Biol. 2005;7:719-23 pubmed
    ..These results provide a link between miRNA function and mammalian P-bodies and suggest that translation repression by RISC delivers mRNAs to P-bodies, either as a cause or as a consequence of inhibiting protein synthesis...
  22. Ding L, Spencer A, Morita K, Han M. The developmental timing regulator AIN-1 interacts with miRISCs and may target the argonaute protein ALG-1 to cytoplasmic P bodies in C. elegans. Mol Cell. 2005;19:437-47 pubmed
    ..These results suggest a model where AIN-1 regulates a subset of miRISCs by localization to the processing bodies, facilitating degradation or translational inhibition of mRNA targets...
  23. Yu J, Yang W, Gulick T, Bloch K, Bloch D. Ge-1 is a central component of the mammalian cytoplasmic mRNA processing body. RNA. 2005;11:1795-802 pubmed
    ..Taken together, the results of this study show that Ge-1 is a central component of P-bodies and suggest that Ge-1 may act prior to the 5(')-decapping step in mRNA degradation...
  24. Brogna S, Ramanathan P, Wen J. UPF1 P-body localization. Biochem Soc Trans. 2008;36:698-700 pubmed publisher
    ..It has been proposed that UPF1 directs PTC-containing mRNAs to P-bodies and triggers decay. Here, we discuss the link between P-bodies and NMD in view of recent studies that suggest that P-bodies are not required for NMD in Drosophila...
  25. O Sullivan M, Swanson P, Knoll J, Taboada E, Dehner L. Undifferentiated embryonal sarcoma with unusual features arising within mesenchymal hamartoma of the liver: report of a case and review of the literature. Pediatr Dev Pathol. 2001;4:482-9 pubmed
    ..We report a case of UES arising in a young boy with MHL, with unusual histologic features, including large mesothelial-lined cysts and ectopic adrenal cortical tissue under Glisson's capsule...
  26. ROBERTS E, Cai F, Kerfeld C, Cannon G, Heinhorst S. Isolation and characterization of the Prochlorococcus carboxysome reveal the presence of the novel shell protein CsoS1D. J Bacteriol. 2012;194:787-95 pubmed publisher
    ..Our study indicates that carboxysome composition is probably more complex than was previously assumed based on the gene complements of the classical cso gene clusters...
  27. Yamada N, Sasaki S, Ishii H, Sato J, Kanno T, Wako Y, et al. Dark cell change of the cerebellar Purkinje cells induced by terbutaline under transient disruption of the blood-brain barrier in adult rats: morphological evaluation. J Appl Toxicol. 2012;32:790-5 pubmed publisher
    ..These findings may suggest that terbutaline induced a failure of Bergmann glial cell and resulted in dark cell degeneration of the Purkinje cells due to glutamate excitotoxicity...
  28. Kahn E, Vejux A, Lizard G, Bessède G, Frouin F, Prunet C, et al. Analysis of the fluorescence of monodansylcadaverine-positive cytoplasmic structures during 7-ketocholesterol-induced cell death. Anal Quant Cytol Histol. 2004;26:47-56 pubmed
    To analyze multilamellar cytoplasmic structures by confocal laser scanning microscopy (CLSM) combined with factor analysis of biomedical image sequences (FAMIS).
  29. Yang X, Dunning K, Wu L, Hickey T, Norman R, Russell D, et al. Identification of perilipin-2 as a lipid droplet protein regulated in oocytes during maturation. Reprod Fertil Dev. 2010;22:1262-71 pubmed publisher
    ..Thus, perilipin-2 is a lipid droplet protein in oocytes and its induction in the COC concurrent with dynamic reorganisation of lipid droplets suggests marked changes in lipid utilisation during oocyte maturation...
  30. Newberg J, Murphy R. A framework for the automated analysis of subcellular patterns in human protein atlas images. J Proteome Res. 2008;7:2300-8 pubmed publisher
    ..The approach described is an important starting point for automatically assigning subcellular locations on a proteome-wide basis for collections of tissue images such as the Atlas...
  31. Schneider M, Najand N, Chaker S, Pare J, Haskins J, Hughes S, et al. Gawky is a component of cytoplasmic mRNA processing bodies required for early Drosophila development. J Cell Biol. 2006;174:349-58 pubmed
    ..This suggests that gw represents a previously unknown member of a small group of genes that need to be expressed zygotically during early embryo development...
  32. Herrera F, Martin V, Carrera P, García Santos G, Rodriguez Blanco J, Rodriguez C, et al. Tryptamine induces cell death with ultrastructural features of autophagy in neurons and glia: Possible relevance for neurodegenerative disorders. Anat Rec A Discov Mol Cell Evol Biol. 2006;288:1026-30 pubmed
    ..1-1 mM). Our results establish a possible link between the role of tryptamine derivatives in brain disorders and the presence of autophagic cell death in these kinds of disorders...
  33. Buchet Poyau K, Courchet J, Le Hir H, Seraphin B, Scoazec J, Duret L, et al. Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies. Nucleic Acids Res. 2007;35:1289-300 pubmed
  34. Albores Saavedra J, Simpson K, Bilello S. The clear cell variant of solid pseudopapillary tumor of the pancreas: a previously unrecognized pancreatic neoplasm. Am J Surg Pathol. 2006;30:1237-42 pubmed
  35. Tilley L, Sougrat R, Lithgow T, Hanssen E. The twists and turns of Maurer's cleft trafficking in P. falciparum-infected erythrocytes. Traffic. 2008;9:187-97 pubmed
    ..Visualization of both resident and cargo proteins has helped decipher the signals and routes for trafficking of parasite proteins to the MC and beyond...
  36. Castejon O. Ultrastructural pathology of neuronal membranes in the oedematous human cerebral cortex. J Submicrosc Cytol Pathol. 2004;36:167-79 pubmed
  37. Ibrahim A, Hutchens H, Berg R, Loesch Fries L. Alfalfa mosaic virus replicase proteins, P1 and P2, localize to the tonoplast in the presence of virus RNA. Virology. 2012;433:449-61 pubmed publisher
    ..This suggests that a functional replication complex containing P1, P2, and a full-length AMV RNA assembles on MVBs to traffic to the tonoplast...
  38. Di Tonno F, Siracusano S, Ciciliato S, Visalli F, Lampropoulou N, Lavelli D. Morphological changes on the intestinal mucosa in orthotopic neobladder. Urol Int. 2012;89:67-70 pubmed publisher
    ..We analyzed the ultrastructural changes of intestinal mucosa in the orthotopic neobladder on the basis of our clinical experience...
  39. Sun Y, Yang P, Zhang Y, Bao X, Li J, Hou W, et al. A genome-wide RNAi screen identifies genes regulating the formation of P bodies in C. elegans and their functions in NMD and RNAi. Protein Cell. 2011;2:918-39 pubmed publisher
    ..Our study offers insights into the cellular mechanisms that regulate the formation of P bodies and also provides a framework for system-level understanding of NMD and RNAi in the context of the development of multicellular organisms...
  40. Da Silveira E, Ribeiro B, Bao S. Characterization of larval haemocytes from the velvetbean caterpillar Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae). J Submicrosc Cytol Pathol. 2003;35:129-39 pubmed
    ..Ultrastructural studies were shown to be useful for the identification and classification of haemocytes, facilitating further analysis by light microscopy...
  41. Bassil E, Hu H, Brown P. Use of phenylboronic acids to investigate boron function in plants. Possible role of boron in transvacuolar cytoplasmic strands and cell-to-wall adhesion. Plant Physiol. 2004;136:3383-95 pubmed
    ..We conclude that boronic acids can be used to rapidly and reversibly induce boron deficiency-like responses and therefore are useful tools for investigating boron function in plants...
  42. Thaler D. The cytoplasmic structure hypothesis for ribosome assembly, vertical inheritance, and phylogeny. Bioessays. 2009;31:774-83 pubmed publisher
    ..e., establish hereditary continuity, will be constrained by the phylogenetic closeness of the cell "body" into which genomes are placed...
  43. Balagopal V, Parker R. Stm1 modulates mRNA decay and Dhh1 function in Saccharomyces cerevisiae. Genetics. 2009;181:93-103 pubmed publisher
    ..These results identify Stm1 as an additional component of the mRNA degradation machinery and suggest a possible connection of mRNA decapping to ribosome function...
  44. Kotaja N, Sassone Corsi P. The chromatoid body: a germ-cell-specific RNA-processing centre. Nat Rev Mol Cell Biol. 2007;8:85-90 pubmed
    ..This highly specialized structure might function as an intracellular focal domain that organizes and controls RNA processing in male germ cells...
  45. Scheller N, Resa Infante P, De La Luna S, Galao R, Albrecht M, Kaestner L, et al. Identification of PatL1, a human homolog to yeast P body component Pat1. Biochim Biophys Acta. 2007;1773:1786-92 pubmed
    ..In addition, as for its yeast counterpart, PatL1 expression was required for P body formation. Taken together, these data emphasize the conservation of important P body components from yeast to human cells...
  46. Katahira J, Miki T, Takano K, Maruhashi M, Uchikawa M, Tachibana T, et al. Nuclear RNA export factor 7 is localized in processing bodies and neuronal RNA granules through interactions with shuttling hnRNPs. Nucleic Acids Res. 2008;36:616-28 pubmed
    ..These findings suggest that NXF7 plays a role in sorting, transport and/or storage of mRNAs through interactions with hnRNP A3...
  47. Robenek H, Severs N. Lipid droplet growth by fusion: insights from freeze-fracture imaging. J Cell Mol Med. 2009;13:4657-61 pubmed publisher
    ..These visual data add significantly to the notion that fusion contributes to lipid droplet growth...
  48. Wang Q, Chen X, Margolis B. PALS1 regulates E-cadherin trafficking in mammalian epithelial cells. Mol Biol Cell. 2007;18:874-85 pubmed
    ..Our results suggest a broad and evolutionarily conserved role for the tight junction protein PALS1 in the biogenesis of adherens junction. ..
  49. Kavanagh S, Schulz T, Davey P, Claudianos C, Russell C, Rathjen P. A family of RS domain proteins with novel subcellular localization and trafficking. Nucleic Acids Res. 2005;33:1309-22 pubmed
    ..Integration of Psc1 into cytospeckles was dependent on the RRM. Cytospeckles were dynamic within the cytoplasm and appeared to traffic into the nucleus. These observations suggest a novel role in RNA metabolism for ARRS proteins...
  50. Gulli M, Jaquenoud M, Shimada Y, Niederhauser G, Wiget P, Peter M. Phosphorylation of the Cdc42 exchange factor Cdc24 by the PAK-like kinase Cla4 may regulate polarized growth in yeast. Mol Cell. 2000;6:1155-67 pubmed
  51. Krishnan B, Truong L. Renal epithelial neoplasms: the diagnostic implications of electron microscopic study in 55 cases. Hum Pathol. 2002;33:68-79 pubmed
    ..The findings lend additional support to the current classification of the pertinent tumor types, facilitate the differential diagnoses, and provide insights into the possible histogenesis of renal epithelial neoplasms...
  52. Mols J, van den Berg A, Otsuka M, Zheng M, Chen J, Han J. TNF-alpha stimulation inhibits siRNA-mediated RNA interference through a mechanism involving poly-(A) tail stabilization. Biochim Biophys Acta. 2008;1779:712-9 pubmed publisher
    ..This is the first report showing that a specific siRNA-mediated mRNA degradation can be regulated by inflammatory stimuli, and that deadenylation is involved in this siRNA-mediated mRNA decay...
  53. Stribinskis V, Ramos K. Rpm2p, a protein subunit of mitochondrial RNase P, physically and genetically interacts with cytoplasmic processing bodies. Nucleic Acids Res. 2007;35:1301-11 pubmed
    ..The results presented here, and the transcriptional activation function demonstrated earlier, implicate Rpm2p as a coordinator of transcription and mRNA storage/decay in P bodies...