canavalia

Summary

Summary: A plant genus of the family FABACEAE. Canavalia ensiformis is the source of CONCANAVALIN A.

Top Publications

  1. Barros P, Stassen H, Freitas M, Carlini C, Nascimento M, Follmer C. Membrane-disruptive properties of the bioinsecticide Jaburetox-2Ec: implications to the mechanism of the action of insecticidal peptides derived from ureases. Biochim Biophys Acta. 2009;1794:1848-54 pubmed publisher
    ..In the light of these findings, for the first time it was possible to point out some evidence that the peptide Jaburetox-2Ec interacting with lipid vesicles promotes membrane permeabilization...
  2. Bezerra G, Oliveira T, Moreno F, de Souza E, da Rocha B, Benevides R, et al. Structural analysis of Canavalia maritima and Canavalia gladiata lectins complexed with different dimannosides: new insights into the understanding of the structure-biological activity relationship in legume lectins. J Struct Biol. 2007;160:168-76 pubmed
    ..In this way, this work presents a crystallographic study of the ConM and CGL (agglutinins from Canavalia maritima and Canavalia gladiata, respectively) in the following complexes: ConM/CGL:Man(alpha1-2)Man(alpha1-O)Me,..
  3. Bezerra W, Carvalho C, Moreira R, Grangeiro T. Establishment of a heterologous system for the expression of Canavalia brasiliensis lectin: a model for the study of protein splicing. Genet Mol Res. 2006;5:216-23 pubmed
    During its biosynthesis in developing Canavalia brasiliensis seeds, the lectin ConBr undergoes a form of protein splicing in which the order of the N- and C-domains of the protein is reversed...
  4. Krajewska B, Ciurli S. Jack bean (Canavalia ensiformis) urease. Probing acid-base groups of the active site by pH variation. Plant Physiol Biochem. 2005;43:651-8 pubmed
    A pH-variation study of jack bean (Canavalia ensiformis) urease steady-state kinetic parameters and of the inhibition constant of boric acid, a urease competitive inhibitor, was performed using both noninhibitory organic (MES, HEPES and ..
  5. Stanisçuaski F, Te Brugge V, Carlini C, Orchard I. Jack bean urease alters serotonin-induced effects on Rhodnius prolixus anterior midgut. J Insect Physiol. 2010;56:1078-86 pubmed publisher
    ..JBU and PGE(2) increase cGMP levels in the anterior midgut, indicating that this molecule might also be part of the JBU pathway...
  6. Havt A, Barbosa P, Sousa T, Martins A, Nobre A, Nascimento K, et al. Renal alterations promoted by the lectins from Canavalia ensiformis (ConA) and Dioclea guianensis (DguiL) seeds. Protein Pept Lett. 2003;10:191-7 pubmed
    The lectin from the seeds of Canavalia ensiformis (ConA) and Dioclea guianensis (DguiL) was tested upon its renal effects using the isolated perfusion rat kidney method...
  7. Becker Ritt A, Martinelli A, Mitidieri S, Feder V, Wassermann G, Santi L, et al. Antifungal activity of plant and bacterial ureases. Toxicon. 2007;50:971-83 pubmed
    ..Herein we demonstrate that (Glycine max) embryo-specific soybean urease, jackbean (Canavalia ensiformis) major urease and a recombinant H...
  8. Moreno F, Delatorre P, Freitas B, Rocha B, Souza E, Facó F, et al. Crystallization and preliminary X-ray diffraction analysis of the lectin from Canavalia gladiata seeds. Acta Crystallogr D Biol Crystallogr. 2004;60:1493-5 pubmed
    The seed lectin from Canavalia gladiata was purified and crystallized. Orthorhombic crystals belonging to space group C222(1) grew within three weeks at 293 K using the hanging-drop vapour-diffusion method...
  9. Mulinari F, Becker Ritt A, Demartini D, Ligabue Braun R, Stanisçuaski F, Verli H, et al. Characterization of JBURE-IIb isoform of Canavalia ensiformis (L.) DC urease. Biochim Biophys Acta. 2011;1814:1758-68 pubmed publisher
    ..Previously, we cloned a cDNA encoding a Canavalia ensiformis urease isoform named JBURE-II, corresponding to a putative smaller urease protein (78kDa) when ..

More Information

Publications82

  1. Jeon K, Na H, Kim Y, Kwon H. Antiangiogenic activity of 4-O-methylgallic acid from Canavalia gladiata, a dietary legume. Biochem Biophys Res Commun. 2005;330:1268-74 pubmed
    ..Herein, we isolated 4-O-methylgallic acid (4-OMGA) from the seed of Canavalia gladiata, a dietary legume, on the basis of the growth inhibitory activity for bovine aortic endothelial cells (..
  2. Balasubramanian A, Ponnuraj K. Purification, crystallization and preliminary X-ray analysis of urease from jack bean (Canavalia ensiformis). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009;65:949-51 pubmed publisher
    ..05 A resolution using synchrotron radiation. The crystals belonged to the hexagonal space group P6(3)22, with unit-cell parameters a = b = 138.57, c = 198.36 A...
  3. Delatorre P, Rocha B, Souza E, Oliveira T, Bezerra G, Moreno F, et al. Structure of a lectin from Canavalia gladiata seeds: new structural insights for old molecules. BMC Struct Biol. 2007;7:52 pubmed
    ..We report the crystal structure of a lectin isolated from Canavalia gladiata seeds (CGL), describing a new binding pocket, which may be related to pathogen resistance activity in ..
  4. Olivera Severo D, Wassermann G, Carlini C. Ureases display biological effects independent of enzymatic activity: is there a connection to diseases caused by urease-producing bacteria?. Braz J Med Biol Res. 2006;39:851-61 pubmed
    ..These findings could be relevant to the elucidation of the role of urease in the pathogenesis of the gastrointestinal disease caused by H. pylori...
  5. Tekiner P, Perçin I, Ergün B, Yavuz H, Aksöz E. Purification of urease from jack bean (Canavalia ensiformis) with copper (II) chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester) cryogels. J Mol Recognit. 2012;25:549-54 pubmed publisher
    Jack bean (Canavalia ensiformis) is the source of interesting proteins that contribute to modern biochemistry, and urease is the primary of these proteins...
  6. Balasubramanian A, Ponnuraj K. Crystal structure of the first plant urease from jack bean: 83 years of journey from its first crystal to molecular structure. J Mol Biol. 2010;400:274-83 pubmed publisher
    ..To date, the structural information is available only for bacterial ureases although the jack bean urease (Canavalia ensiformis; JBU), the best-studied plant urease, was the first enzyme to be crystallized in 1926...
  7. Defferrari M, Demartini D, Marcelino T, Pinto P, Carlini C. Insecticidal effect of Canavalia ensiformis major urease on nymphs of the milkweed bug Oncopeltus fasciatus and characterization of digestive peptidases. Insect Biochem Mol Biol. 2011;41:388-99 pubmed publisher
    Jackbean (Canavalia ensiformis) ureases are entomotoxic upon the release of internal peptides by insect's digestive enzymes...
  8. Nunes B, Rensonnet N, Dal Secco D, Vieira S, Cavada B, Teixeira E, et al. Lectin extracted from Canavalia grandiflora seeds presents potential anti-inflammatory and analgesic effects. Naunyn Schmiedebergs Arch Pharmacol. 2009;379:609-16 pubmed publisher
    ..In this study, we addressed the potential use of a lectin from Canavalia grandiflora seeds (ConGF) to control neutrophil migration and inflammatory hypernociception...
  9. Karmali K, Karmali A, Teixeira A, Curto M. The use of Fourier transform infrared spectroscopy to assay for urease from Pseudomonas aeruginosa and Canavalia ensiformis. Anal Biochem. 2004;331:115-21 pubmed
    A novel assay method was investigated for urease (EC 3.5.1.5) from Pseudomonas aeruginosa and Canavalia ensiformis by Fourier transform infrared spectroscopy...
  10. Gadelha C, Moreno F, Santi Gadelha T, Cajazeiras J, Rocha B, Assreuy A, et al. Native crystal structure of a nitric oxide-releasing lectin from the seeds of Canavalia maritima. J Struct Biol. 2005;152:185-94 pubmed
    Here, we report the crystallographic study of a lectin from Canavalia maritima seeds (ConM) and its relaxant activity on vascular smooth muscle, to provide new insights into the understanding of structure/function relationships of this ..
  11. Delatorre P, Rocha B, Gadelha C, Santi Gadelha T, Cajazeiras J, Souza E, et al. Crystal structure of a lectin from Canavalia maritima (ConM) in complex with trehalose and maltose reveals relevant mutation in ConA-like lectins. J Struct Biol. 2006;154:280-6 pubmed
    The crystal structure of Canavalia maritima lectin (ConM) complexed with trehalose and maltose revealed relevant point mutations in ConA-like lectins...
  12. Ashiralieva A, Kleiner D. Polyhalogenated benzo- and naphthoquinones are potent inhibitors of plant and bacterial ureases. FEBS Lett. 2003;555:367-70 pubmed
    ..benzo- and naphthoquinones were found to be potent inhibitors of pure ureases from Bacillus pasteurii and Canavalia ensiformis...
  13. Dwevedi A, Routh S, Yadav A, Singh A, Srivastava O, Kayastha A. Response surface analysis of nano-ureases from Canavalia ensiformis and Cajanus cajan. Int J Biol Macromol. 2011;49:674-80 pubmed publisher
    Ureases isolated from leguminous sources, Canavalia ensiformis and Cajanus cajan were immobilized onto gold nanoparticles (nano-ureases)...
  14. Sivapriya K, Suguna P, Banerjee A, Saravanan V, Rao D, Chandrasekaran S. Facile one-pot synthesis of thio and selenourea derivatives: a new class of potent urease inhibitors. Bioorg Med Chem Lett. 2007;17:6387-91 pubmed
    ..The compounds were tested for their activity as urease inhibitors and some of the compounds showed potent activity in the nanomolar range towards jack bean urease...
  15. Marlier J, Cleland W. Multiple isotope effect study of the hydrolysis of formamide by urease from jack bean (Canavalia ensiformis). Biochemistry. 2006;45:9940-8 pubmed
    ..For urea, the preference is for the anionic form of the sulfhydryl; for formamide, the neutral form is preferred, leading to the lower pH optimum...
  16. Demartini D, Wlodawer A, Carlini C. A comparative study of the expression of serine proteinases in quiescent seeds and in developing Canavalia ensiformis plants. J Exp Bot. 2007;58:521-32 pubmed
    An alkaline proteinase activity is present in quiescent seeds and up to the 24th day of development of Canavalia ensiformis DC (L.) plants...
  17. Zambelli B, Musiani F, Benini S, Ciurli S. Chemistry of Ni2+ in urease: sensing, trafficking, and catalysis. Acc Chem Res. 2011;44:520-30 pubmed publisher
  18. Piovesan A, Stanisçuaski F, Marco Salvadori J, Real Guerra R, Defferrari M, Carlini C. Stage-specific gut proteinases of the cotton stainer bug Dysdercus peruvianus: role in the release of entomotoxic peptides from Canavalia ensiformis urease. Insect Biochem Mol Biol. 2008;38:1023-32 pubmed publisher
    b>Canavalia ensiformis ureases are toxic to insects of different orders. The entomotoxicity of urease is due to a 10 kDa internal peptide released by proteinases in the insect digestive tract...
  19. Mulinari F, Stanisçuaski F, Bertholdo Vargas L, Postal M, Oliveira Neto O, Rigden D, et al. Jaburetox-2Ec: an insecticidal peptide derived from an isoform of urease from the plant Canavalia ensiformis. Peptides. 2007;28:2042-50 pubmed
    Canatoxin, a urease isoform from Canavalia ensiformis seeds, shows insecticidal activity against different insect species...
  20. Peters W, Knoblauch M, Warmann S, Schnetter R, Shen A, Pickard W. Tailed forisomes of Canavalia gladiata: a new model to study Ca2+-driven protein contractility. Ann Bot. 2007;100:101-9 pubmed
    ..Previous work employed Vicia faba forisomes, a not entirely unproblematic experimental system. The aim of this study was to seek to establish a superior model to study these intriguing actuators...
  21. Jacques A, Rieger D, Maestri M, Lopes M, Peres T, Gonçalves F, et al. Lectin from Canavalia brasiliensis (ConBr) protects hippocampal slices against glutamate neurotoxicity in a manner dependent of PI3K/Akt pathway. Neurochem Int. 2013;62:836-42 pubmed publisher
    ..ConBr is a mannose/glucose-binding lectin purified from Canavalia brasiliensis seeds...
  22. Wong J, Ng T. Isolation and characterization of a glucose/mannose/rhamnose-specific lectin from the knife bean Canavalia gladiata. Arch Biochem Biophys. 2005;439:91-8 pubmed
    ..with specificity toward mannose, glucose, and rhamnose has been isolated from the legumes of the knife bean Canavalia gladiata...
  23. Russi M, Vandresen Filho S, Rieger D, Costa A, Lopes M, Cunha R, et al. ConBr, a lectin from Canavalia brasiliensis seeds, protects against quinolinic acid-induced seizures in mice. Neurochem Res. 2012;37:288-97 pubmed publisher
    ..ConBr, a lectin isolated from the seeds of Canavalia brasiliensis, previously showed antidepressant effect in the forced swimming test in mice, with involvement of ..
  24. de Vasconcelos M, Cunha C, Arruda F, Carneiro V, Mercante F, do Nascimento Neto L, et al. Lectin from Canavalia brasiliensis seeds (ConBr) is a valuable biotechnological tool to stimulate the growth of Rhizobium tropici in vitro. Molecules. 2012;17:5244-54 pubmed publisher
    To study the interactions between a Rhizobium tropici strain and lectins isolated from the seeds of Canavalia ensiformis (ConA) and Canavalia brasiliensis (ConBr), a lectin fluorescence assay was performed...
  25. Bezerra E, Rocha B, Nagano C, Bezerra G, Moura T, Bezerra M, et al. Structural analysis of ConBr reveals molecular correlation between the carbohydrate recognition domain and endothelial NO synthase activation. Biochem Biophys Res Commun. 2011;408:566-70 pubmed publisher
    ..Here we report a new lectin crystal structure (ConBr) extracted from seeds of Canavalia brasiliensis, predict dimannoside binding by docking, identify the ?-aminobutyric acid (Abu) binding pocket and ..
  26. Jin Y, Zhang T, Samaranayake Y, Fang H, Yip H, Samaranayake L. The use of new probes and stains for improved assessment of cell viability and extracellular polymeric substances in Candida albicans biofilms. Mycopathologia. 2005;159:353-60 pubmed
    ..We therefore assessed the utility of two different labeled lectins Erythrina cristagalli (ECA) and Canavalia ensiformis (ConA), for EPS visualization...
  27. Andrade S, Gratão P, Schiavinato M, Silveira A, Azevedo R, Mazzafera P. Zn uptake, physiological response and stress attenuation in mycorrhizal jack bean growing in soil with increasing Zn concentrations. Chemosphere. 2009;75:1363-70 pubmed publisher
    The influence of arbuscular mycorrhizal fungi (AMF) inoculation on Canavalia ensiformis growth, nutrient and Zn uptake, and on some physiological parameters in response to increasing soil Zn concentrations was studied...
  28. Packer J, Naz T, Harrington D, Jamie J, Vemulpad S. Antimicrobial activity of customary medicinal plants of the Yaegl Aboriginal community of northern New South Wales, Australia: a preliminary study. BMC Res Notes. 2015;8:276 pubmed publisher
    ..previously documented medicinal applications, aqueous and aqueous ethanolic extracts of Alocasia brisbanensis, Canavalia rosea, Corymbia intermedia, Hibbertia scandens, Ipomoea brasiliensis, Lophostemon suaveolens and Syncarpia ..
  29. Zhang H, Cui W, Wen J, Blankenship R, Gross M. Native electrospray and electron-capture dissociation FTICR mass spectrometry for top-down studies of protein assemblies. Anal Chem. 2011;83:5598-606 pubmed publisher
    ..The approach provides not only top-down proteomics information of the complex subunits but also structural insights complementary to those obtained by ion mobility. ..
  30. Nazari K, Mahmoudi A, Esmaeili N, Sadeghian L, Moosavi Movahedi A, Khodafarin R. Denaturation of jack-bean urease by sodium n-dodecyl sulphate: a kinetic study below the critical micelle concentration. Colloids Surf B Biointerfaces. 2006;53:139-48 pubmed
    ..Electrophoresis experiments on the native and surfactant/urease complexes indicated a higher mobility for the kinetically folded native like intermediate. ..
  31. Kaushik S, Mohanty D, Surolia A. The role of metal ions in substrate recognition and stability of concanavalin A: a molecular dynamics study. Biophys J. 2009;96:21-34 pubmed publisher
    The binding of carbohydrate substrates to concanavalin A (Canavalia ensiformis agglutinin (ConA)) is essential for its interaction with various glycoproteins...
  32. Yabuuchi S, Endo S, Baek K, Hoshino K, Tsujino Y, Vestergaard M, et al. Raft-dependent endocytic movement and intracellular cluster formation during T cell activation triggered by concanavalin A. J Biosci Bioeng. 2017;124:685-693 pubmed publisher
    Certain food ingredients can stimulate the human immune system. A lectin, concanavalin A (ConA), from Canavalia ensiformis (jack bean) is one of the most well-known food-derived immunostimulants and mediates activation of cell-mediated ..
  33. Carrazoni T, de Avila Heberle M, Perin A, Zanatta A, Rodrigues P, Dos Santos F, et al. Central and peripheral neurotoxicity induced by the Jack Bean Urease (JBU) in Nauphoeta cinerea cockroaches. Toxicology. 2016;368-369:162-171 pubmed publisher
    Ureases of Canavalia ensiformis are natural insecticides with a still elusive entomotoxic mode of action. We have investigated the mechanisms involved in the neurotoxicity induced by Jack Bean Urease (JBU) in Nauphoeta cinerea (Olivier)...
  34. Kaefer C, Komninou E, Campos V, de Leon P, Arruda F, Nascimento K, et al. Binding pattern and toxicological effects of lectins from genus Canavalia on bovine sperm. Reprod Toxicol. 2013;38:72-80 pubmed publisher
    The aim of this study was to determine the binding patterns of Canavalia ensiformis (ConA), Canavalia boliviana (ConBol) and Canavalia brasiliensis (ConBr) lectins to bovine sperm and their effects on sperm motility, viability, lipid ..
  35. Kot M, Karcz W, Zaborska W. 5-Hydroxy-1,4-naphthoquinone (juglone) and 2-hydroxy-1,4-naphthoquinone (lawsone) influence on jack bean urease activity: Elucidation of the difference in inhibition activity. Bioorg Chem. 2010;38:132-7 pubmed publisher
    ..Thus, this indicated a significance of a steric hindrance which appeared when the hydroxyl group changing position from 5 in juglone (5-hydroxy-1,4-naphthoquinone) to 2 in lawsone (2-hydroxy-1,4-naphthoquinone). ..
  36. Soares P, Nascimento C, Porto T, Correia M, Porto A, Carneiro da Cunha M. Purification of a lectin from Canavalia ensiformis using PEG-citrate aqueous two-phase system. J Chromatogr B Analyt Technol Biomed Life Sci. 2011;879:457-60 pubmed publisher
    ..A (Con A) and subsequently applied to the extraction and purification of Con A from the crude extract of Canavalia ensiformis seeds...
  37. Suman -, O Reilly E, Kelly M, Morrin A, Smyth M, Killard A. Chronocoulometric determination of urea in human serum using an inkjet printed biosensor. Anal Chim Acta. 2011;697:98-102 pubmed publisher
    ..85. Bland-Altman plots showed that in the range of 5.8-6.6 mM urea, the developed sensor had an average positive experimental bias of 0.12 mM (<2% RSD) over the reference method. ..
  38. Filiz E, Vatansever R, Ozyigit I. Molecular docking of Glycine max and Medicago truncatula ureases with urea; bioinformatics approaches. Mol Biol Rep. 2016;43:129-40 pubmed publisher
    ..whose crystallographic structures are not available, along with three additional solved urease structures from Canavalia ensiformis (PDB: 4GY7), Bacillus pasteurii (PDB: 4UBP) and Klebsiella aerogenes (PDB: 1FWJ)...
  39. Li C, Xie J, Chen X, Mo Z, Wu W, Liang Y, et al. Comparison of Helicobacter pylori Urease Inhibition by Rhizoma Coptidis, Cortex Phellodendri and Berberine: Mechanisms of Interaction with the Sulfhydryl Group. Planta Med. 2016;82:305-11 pubmed publisher
    ..The concentration- and time-dependent, reversible, and noncompetitive inhibition against urease by C. chinensis might be attributed to its interaction with the sulfhydryl group of the active site of urease. ..
  40. Zaborska W, Kot M, Bala A. Kinetics of jack bean urease inhibition by 2,3-dichloro-1,4-naphthoquinone. Elucidation of the mechanism: redox cycling and sulfhydryl arylation. J Enzyme Inhib Med Chem. 2009;24:1082-7 pubmed publisher
  41. Seena S, Sridhar K. Endophytic fungal diversity of 2 sand dune wild legumes from the southwest coast of India. Can J Microbiol. 2004;50:1015-21 pubmed
    ..plants) and 5 tissue classes (cotyledons, seed coats, roots, stems, and leaves) of coastal sand dune legumes Canavalia cathartica and Canavalia maritima were assessed by plating surface-sterilized segments on malt extract agar...
  42. McDuff F, Doucet A, Beauregard M. Low concentration of guanidine hydrochloride induces the formation of an aggregation-prone state in alpha-urease. Biochem Cell Biol. 2004;82:305-13 pubmed
    b>Canavalia ensiformis (jack bean) alpha-urease is a hexameric protein characterized by a complex denaturation mechanism...
  43. Gondim A, Romero Canelón I, Sousa E, Blindauer C, Butler J, Romero M, et al. The potent anti-cancer activity of Dioclea lasiocarpa lectin. J Inorg Biochem. 2017;175:179-189 pubmed publisher
    ..ovarian), A549 (lung), MCF-7 (breast) and PC3 (prostate), and was as, or more, potent than the lectins ConBr (Canavalia brasiliensis), ConM (Canavalia maritima) and DSclerL (Dioclea sclerocarpa) against A2780 and PC3 cells...
  44. Saeed A, Khan M, Rafique H, Shahid M, Iqbal J. Design, synthesis, molecular docking studies and in vitro screening of ethyl 4-(3-benzoylthioureido) benzoates as urease inhibitors. Bioorg Chem. 2014;52:1-7 pubmed publisher
    ..13 ?M, respectively), and was 100-fold more potent than the standard inhibitors. Further molecular docking studies were carried out using the crystal structure of urease to find out the binding mode of the inhibitors with the enzyme. ..
  45. Zamora N. [Effect of extrusion on the activity of anti-nutritional factors and in vitro digestibility of protein and starch in flours of Canavalia ensiformis]. Arch Latinoam Nutr. 2003;53:293-8 pubmed
    ..155 degrees C, 20% moisture, screw speed 75 rpm, feed speed 205 g min-1) on antinutritional factors of Canavalia ensiformis was studied. In vitro protein and starch digestibilities were assessed...
  46. Yoshida H, Yoshida N, Kuriyama I, Tomiyama Sakamoto Y, Mizushina Y. Profiles of lipid components, fatty acid distributions of triacylglycerols and phospholipids in Jack beans (Canavalia gladiata DC.). Food Chem. 2013;136:807-12 pubmed publisher
    Endogenous tocopherols in extracted lipids from Jack beans (Canavalia gladiata DC...
  47. Adsul L, Bandgar B, Chavan H, Jalde S, Dhakane V, Shirfule A. Synthesis and biological evaluation of novel series of aminopyrimidine derivatives as urease inhibitors and antimicrobial agents. J Enzyme Inhib Med Chem. 2013;28:1316-23 pubmed publisher
    ..All the compounds were subjected for ADME predictions by computational method...
  48. Cavada B, Iglesias M, Troncoso M, Teixeira E, Turyn D, Dominici F. Glucose-mannose-binding lectins isolated from Brazilian beans stimulate the autophosphorylation of the insulin receptor in vitro. Horm Metab Res. 2003;35:125-7 pubmed
  49. Sinha S, Mitra N, Kumar G, Bajaj K, Surolia A. Unfolding studies on soybean agglutinin and concanavalin a tetramers: a comparative account. Biophys J. 2005;88:1300-10 pubmed
    ..These interactions further stabilize SBA with respect to ConA, which is not glycosylated...
  50. Ribeiro E, Uchôa A, da Silva L, Beghini D, Ferreira A, Perales J, et al. Insulin-binding canavalin is present in Canavalia ensiformis seed coat. Protein Pept Lett. 2009;16:1106-11 pubmed
    An insulin-binding protein was isolated from Canavalia ensiformis seed coat, by using an insulin-Sepharose 4B affinity chromatography, and the protein was identified as canavalin (Canavalia 7S globulin) by mass spectrometry analysis...
  51. Pei Z, Anderson H, Aastrup T, Ramström O. Study of real-time lectin-carbohydrate interactions on the surface of a quartz crystal microbalance. Biosens Bioelectron. 2005;21:60-6 pubmed
    ..18 to 5.3 mM, in good correlation with a related enzyme-labelled lectin assay (ELLA) protocol...
  52. Kot M, Zaborska W. Inhibition of jack bean urease by tetrachloro-o-benzoquinone and tetrachloro-p-benzoquinone. J Enzyme Inhib Med Chem. 2006;21:537-42 pubmed
    ..No recovery of urease activity bound in the urease-inhibitor complexes proves that the complexes are stable and strong...
  53. Pattamadilok D, Pengsuparp T, Phummiratch D, Ongpipattanakul B, Meksuriyen D, Kawanishi K, et al. Canarosine: a new guanidine alkaloid from Canavalia rosea with inhibitory activity on dopamine D1 receptors. J Asian Nat Prod Res. 2008;10:915-8 pubmed publisher
    ..3), daucosterol (4), epi-inositol 6-O-methyl ether (5), and rutin (6), were isolated from the aerial parts of Canavalia rosea. Their structures were established on the basis of their spectroscopic data...
  54. Saeed A, Larik F, Channar P, Mehfooz H, Ashraf M, Abbas Q, et al. An expedient synthesis of N-(1-(5-mercapto-4-((substituted benzylidene)amino)-4H-1,2,4-triazol-3-yl)-2-phenylethyl)benzamides as jack bean urease inhibitors and free radical scavengers: Kinetic mechanism and molecular docking studies. Chem Biol Drug Des. 2017;90:764-777 pubmed publisher
    ..The results of enzyme inhibition, kinetic mechanism and molecular docking suggest that these compounds can serve as lead compounds in the design of more effective urease inhibitors...
  55. Azem A, Tsfadia Y, Hajouj O, Shaked I, Daniel E. Cross-linking with bifunctional reagents and its application to the study of the molecular symmetry and the arrangement of subunits in hexameric protein oligomers. Biochim Biophys Acta. 2010;1804:768-80 pubmed publisher
    ..The conclusions from cross-linking of GDH and GDC, PpHc and rho are consistent with results from X-ray crystal structure, those for urease with findings from electron microscopy...
  56. de Freitas Pires A, Assreuy A, Lopes E, Celedônio N, Soares C, Rodrigues N, et al. Opioid-like antinociceptive effects of oral administration of a lectin purified from the seeds of Canavalia brasiliensis. Fundam Clin Pharmacol. 2013;27:201-9 pubmed publisher
    The objective of this study was to evaluate the antinociceptive effects of a lectin from Canavalia brasiliensis (ConBr) when administered orally to murine models of chemical and thermal nociception...
  57. Stanisçuaski F, Ferreira Dasilva C, Mulinari F, Pires Alves M, Carlini C. Insecticidal effects of canatoxin on the cotton stainer bug Dysdercus peruvianus (Hemiptera: Pyrrhocoridae). Toxicon. 2005;45:753-60 pubmed
    Canatoxin (CNTX) is a variant form of urease isolated from Canavalia ensiformis (Leguminosaea) seeds...
  58. Teixeira C, Cavassani K, Gomes R, Teixeira M, Roque Barreira M, Cavada B, et al. Potential of KM+ lectin in immunization against Leishmania amazonensis infection. Vaccine. 2006;24:3001-8 pubmed
    In the present study we evaluated Canavalia brasiliensis (ConBr), Pisum arvense (PAA) and Artocarpus integrifolia (KM+) lectins as immunostimulatory molecules in vaccination against Leishmania amazonensis infection...
  59. Dowd P, Johnson E, Pinkerton T. Oral toxicity of beta-N-acetyl hexosaminidase to insects. J Agric Food Chem. 2007;55:3421-8 pubmed
    ..Commercial beta-N-acetylhexosaminidase (NAHA), a chitin-degrading enzyme from jack bean Canavalia ensiformis, caused significant mortality of fall armyworm Spodoptera frugiperda larvae at 75 microg/gm, but no ..
  60. Ternovskiĭ V, Chernokhvostov I, Fomkina M, Montrel M. [A potentiometric sensor designed on the basis of urease immobilized in polyelectrolyte microcapsules]. Biofizika. 2007;52:825-9 pubmed
    ..The sensor has been shown to be stable for at least three weeks. The standard curves of the sensor are linear in the range of urea concentrations from 0.2 to 20 mM...
  61. Ekanayake S, Nair B, Jansz E, Asp N. Effect of processing on the protein nutritional value of Canavalia gladiata seeds. Nahrung. 2003;47:256-60 pubmed
    ..TD), biological value (BV), and net protein utilization (NPU) of diets containing mature sword bean (Canavalia gladiata), seed flour and grits were carried out with male Sprague-Dawley rats...
  62. Krajewska B, van Eldik R, Brindell M. Temperature- and pressure-dependent stopped-flow kinetic studies of jack bean urease. Implications for the catalytic mechanism. J Biol Inorg Chem. 2012;17:1123-34 pubmed publisher
    ..Structure 7:205-216; 1999), in which the Ni-Ni bridging hydroxide--not the terminal hydroxide--is the nucleophile in the catalytic reaction...
  63. Cruz Hipolito H, Osuna M, Heredia A, Ruiz Santaella J, De Prado R. Nontarget mechanims involved in glyphosate tolerance found in Canavalia ensiformis plants. J Agric Food Chem. 2009;57:4844-8 pubmed publisher
    A glyphosate-tolerant population of Canavalia ensiformis was collected in a cover crop in citrus orchards in Veracruz (Mexico), where glyphosate had been used for the first time. A susceptible Amaranthus hybridus L...
  64. Wicklein B, Darder M, Aranda P, Ruiz Hitzky E. Phospholipid-sepiolite biomimetic interfaces for the immobilization of enzymes. ACS Appl Mater Interfaces. 2011;3:4339-48 pubmed publisher
    ..The results emphasize the good preservation of bioactivity due to the accommodation of the enzymatic system within the biomimetic lipid interface on sepiolite...
  65. Roy B, Das T, Maiti T, Chakraborty S. Effect of fluidic transport on the reaction kinetics in lectin microarrays. Anal Chim Acta. 2011;701:6-14 pubmed publisher
    ..The exploration, therefore, reveals a unique coupling between reaction kinetics and hydrodynamics for biomacromolecules and provides a generic scheme towards futuristic microfluidics-coupled biomedical assays...
  66. Krajewska B. Mono- (Ag, Hg) and di- (Cu, Hg) valent metal ions effects on the activity of jack bean urease. Probing the modes of metal binding to the enzyme. J Enzyme Inhib Med Chem. 2008;23:535-42 pubmed
  67. Schneider C, Shukla D, Trout B. Effects of solute-solute interactions on protein stability studied using various counterions and dendrimers. PLoS ONE. 2011;6:e27665 pubmed publisher
    ..Attractive guanidinium-anion interactions seem to cause clustering in solution, which inhibits cooperative binding to the protein surface but at the same time, significantly suppresses nonnative aggregation...
  68. Santos S, Moraes M, Rezende M. Determination of polyamines in organic extracts from roots of Canavalia ensiformis by capillary electrophoresis. J Environ Sci Health B. 2010;45:325-9 pubmed publisher
    ..and specific capillary zone electrophoresis method to determine polyamines in organic extracts from roots of Canavalia ensiformis (Jack Beans) was developed using ultra violet (UV) detection...
  69. Iftikhar F, Ali Y, Ahmad Kiani F, Fahad Hassan S, Fatima T, Khan A, et al. Design, synthesis, in vitro Evaluation and docking studies on dihydropyrimidine-based urease inhibitors. Bioorg Chem. 2017;74:53-65 pubmed publisher
    ..The overall results of urease inhibition have shown that these compounds can be further optimized and developed as lead urease inhibitors. ..
  70. Zheng H, Du X. Multivalent protein binding in carbohydrate-functionalized monolayers through protein-directed rearrangement and reorientation of glycolipids at the air-water interface. Biochim Biophys Acta. 2011;1808:2128-35 pubmed publisher
    ..The directed rearranged binary monolayers with multivalent protein binding were preserved for the preparation of biosensors...
  71. Gadelha C, Moreno F, Santi Gadelha T, Cajazeiras J, Rocha B, Rustiguel J, et al. Crystallization and preliminary X-ray diffraction analysis of a lectin from Canavalia maritima seeds. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005;61:87-9 pubmed
    A lectin from Canavalia maritima seeds (ConM) was purified and submitted to crystallization experiments. The best crystals were obtained using the vapour-diffusion method at a constant temperature of 293 K and grew in 7 d...
  72. Piovesan A, Martinelli A, Ligabue Braun R, Schwartz J, Carlini C. Canavalia ensiformis urease, Jaburetox and derived peptides form ion channels in planar lipid bilayers. Arch Biochem Biophys. 2014;547:6-17 pubmed publisher
    ..e., platelet and neutrophil activation, fungus inhibition and insecticidal effect. Urease from Canavalia ensiformis (jack bean) is toxic to several hemipteran and coleopteran insects...
  73. Ingram C, Coyne M, Williams D. Effects of commercial diazinon and imidacloprid on microbial urease activity in soil and sod. J Environ Qual. 2005;34:1573-80 pubmed
    ..sod. Bacterial urease from Bacillus pasteurii and plant urease from jack bean [Canavalia ensiformis (L.) DC.] were unaffected by the insecticides...