chlamydomonas reinhardtii

Summary

Summary: A species of GREEN ALGAE. Delicate, hairlike appendages arise from the flagellar surface in these organisms.

Top Publications

  1. Almeida C, Pereira C, da Costa D, Pereira S, Pissarra J, Simões I, et al. Chlapsin, a chloroplastidial aspartic proteinase from the green algae Chlamydomonas reinhardtii. Planta. 2012;236:283-96 pubmed publisher
    ..Here we report on the identification of the first (and only) typical aspartic proteinase from Chlamydomonas reinhardtii. This enzyme, named chlapsin, was shown to maintain the primary structure organization of typical plant ..
  2. Burgess S, Tredwell G, Moln r A, Bundy J, Nixon P. Artificial microRNA-mediated knockdown of pyruvate formate lyase (PFL1) provides evidence for an active 3-hydroxybutyrate production pathway in the green alga Chlamydomonas reinhardtii. J Biotechnol. 2012;162:57-66 pubmed publisher
    ..microRNA technology was investigated as a means of down regulating metabolic pathways in the green alga Chlamydomonas reinhardtii, targeting pyruvate formate lyase (PFL1), which catalyzes the conversion of pyruvate to acetyl-CoA and ..
  3. Piccapietra F, Allué C, Sigg L, Behra R. Intracellular silver accumulation in Chlamydomonas reinhardtii upon exposure to carbonate coated silver nanoparticles and silver nitrate. Environ Sci Technol. 2012;46:7390-7 pubmed publisher
    ..nitrate (20-500 nM) was examined in the wild type and in the cell wall free mutant of the green alga Chlamydomonas reinhardtii at pH 7.5...
  4. Lauersen K, Berger H, Mussgnug J, Kruse O. Efficient recombinant protein production and secretion from nuclear transgenes in Chlamydomonas reinhardtii. J Biotechnol. 2013;167:101-10 pubmed publisher
    ..the development of an advanced RP production and secretion system for the green unicellular model alga Chlamydomonas reinhardtii. We generated a versatile expression vector that employs the secretion signal of the native ..
  5. Scognamiglio V, Stano P, Polticelli F, Antonacci A, Lambreva M, Pochetti G, et al. Design and biophysical characterization of atrazine-sensing peptides mimicking the Chlamydomonas reinhardtii plastoquinone binding niche. Phys Chem Chem Phys. 2013;15:13108-15 pubmed publisher
    ..In this work, the Chlamydomonas reinhardtii D1 primary structure was used as a template to computationally design novel peptides enabling the ..
  6. Rahire M, Laroche F, Cerutti L, Rochaix J. Identification of an OPR protein involved in the translation initiation of the PsaB subunit of photosystem I. Plant J. 2012;72:652-61 pubmed publisher
    ..Tab 1 is part of a large family of proteins in Chlamydomonas that are also found in several bacteria and protozoans, but are rare in land plants. ..
  7. Komsic Buchmann K, Stephan L, Becker B. The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci. 2012;125:2885-95 pubmed publisher
    ..In addition, CreSEC6 is not only necessary for CV function, but possibly influences the CV cycle in an indirect manner and flagellar length in Chlamydomonas...
  8. Fang W, Si Y, Douglass S, Casero D, Merchant S, Pellegrini M, et al. Transcriptome-wide changes in Chlamydomonas reinhardtii gene expression regulated by carbon dioxide and the CO2-concentrating mechanism regulator CIA5/CCM1. Plant Cell. 2012;24:1876-93 pubmed publisher
    We used RNA sequencing to query the Chlamydomonas reinhardtii transcriptome for regulation by CO(2) and by the transcription regulator CIA5 (CCM1)...
  9. Tokutsu R, Minagawa J. Energy-dissipative supercomplex of photosystem II associated with LHCSR3 in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2013;110:10016-21 pubmed publisher
    ..Recently, nonphotochemical quenching 4 (npq4), a mutant strain of the green alga Chlamydomonas reinhardtii that is qE-deficient and lacks the light-harvesting complex stress-related protein 3 (LHCSR3), was ..

More Information

Publications86

  1. Wobbe L, Nixon P. The mTERF protein MOC1 terminates mitochondrial DNA transcription in the unicellular green alga Chlamydomonas reinhardtii. Nucleic Acids Res. 2013;41:6553-67 pubmed publisher
    ..b>Chlamydomonas reinhardtii is a unicellular green alga widely used as a model organism for photosynthesis research and green ..
  2. Brumfield K, Moroney J, Moore T, Simms T, Donze D. Functional characterization of the Chlamydomonas reinhardtii ERG3 ortholog, a gene involved in the biosynthesis of ergosterol. PLoS ONE. 2010;5:e8659 pubmed publisher
    The predominant sterol in the membranes of the alga Chlamydomonas reinhardtii is ergosterol, which is commonly found in the membranes of fungi, but is rarely found in higher plants...
  3. Gupta A, Diener D, Sivadas P, Rosenbaum J, Yang P. The versatile molecular complex component LC8 promotes several distinct steps of flagellar assembly. J Cell Biol. 2012;198:115-26 pubmed publisher
    ..These multiple effects shed new light on fundamental questions about LC8-containing complexes and axoneme assembly...
  4. Tran M, Van C, Barrera D, Pettersson P, Peinado C, Bui J, et al. Production of unique immunotoxin cancer therapeutics in algal chloroplasts. Proc Natl Acad Sci U S A. 2013;110:E15-22 pubmed publisher
    ..The chloroplast of the green alga Chlamydomonas reinhardtii has been shown to contain the machinery necessary to fold and assemble complex eukaryotic proteins...
  5. Sinetova M, Kupriyanova E, Markelova A, Allakhverdiev S, Pronina N. Identification and functional role of the carbonic anhydrase Cah3 in thylakoid membranes of pyrenoid of Chlamydomonas reinhardtii. Biochim Biophys Acta. 2012;1817:1248-55 pubmed publisher
    ..associated with thylakoid membranes in the chloroplast and pyrenoid was studied in wild-type cells of Chlamydomonas reinhardtii and in its cia3 mutant deficient in the activity of the Cah3 protein...
  6. Catalanotti C, Dubini A, Subramanian V, Yang W, Magneschi L, Mus F, et al. Altered fermentative metabolism in Chlamydomonas reinhardtii mutants lacking pyruvate formate lyase and both pyruvate formate lyase and alcohol dehydrogenase. Plant Cell. 2012;24:692-707 pubmed publisher
    b>Chlamydomonas reinhardtii, a unicellular green alga, often experiences hypoxic/anoxic soil conditions that activate fermentation metabolism...
  7. Pillai S, Behra R, Nestler H, Suter M, Sigg L, Schirmer K. Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver. Proc Natl Acad Sci U S A. 2014;111:3490-5 pubmed publisher
    ..The effects of silver were investigated at the transcriptome, proteome, and cellular levels of Chlamydomonas reinhardtii. The cells instigate a fast transcriptome and proteome response, including perturbations in copper ..
  8. Tsai Y, Mueller Cajar O, Saschenbrecker S, Hartl F, Hayer Hartl M. Chaperonin cofactors, Cpn10 and Cpn20, of green algae and plants function as hetero-oligomeric ring complexes. J Biol Chem. 2012;287:20471-81 pubmed publisher
    ..In the green alga Chlamydomonas reinhardtii (Cr), three genes encode chaperonin cofactors, with cpn10 encoding a single ?10-kDa domain and cpn20 and ..
  9. Jones C, Luong T, Hannon M, Tran M, Gregory J, Shen Z, et al. Heterologous expression of the C-terminal antigenic domain of the malaria vaccine candidate Pfs48/45 in the green algae Chlamydomonas reinhardtii. Appl Microbiol Biotechnol. 2013;97:1987-95 pubmed publisher
    ..Recently, the chloroplast of Chlamydomonas reinhardtii has been used to express complex recombinant proteins...
  10. Oda T, Kikkawa M. Novel structural labeling method using cryo-electron tomography and biotin-streptavidin system. J Struct Biol. 2013;183:305-311 pubmed publisher
    ..In conclusion, the combination of the biotin-streptavidin system and cryo-electron tomography is a powerful method to investigate the structure of large macromolecular complexes. ..
  11. Chen H, Melis A. Marker-free genetic engineering of the chloroplast in the green microalga Chlamydomonas reinhardtii. Plant Biotechnol J. 2013;11:818-28 pubmed publisher
    ..transgene expression method based on the replacement of an inactive rbcL gene as the selection marker in Chlamydomonas reinhardtii chloroplasts...
  12. Volgusheva A, Styring S, Mamedov F. Increased photosystem II stability promotes H2 production in sulfur-deprived Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2013;110:7223-8 pubmed publisher
    Photobiological H2 production is an attractive option for renewable solar fuels. Sulfur-deprived cells of Chlamydomonas reinhardtii have been shown to produce hydrogen with the highest efficiency among photobiological systems...
  13. Lechtreck K, Brown J, Sampaio J, Craft J, Shevchenko A, Evans J, et al. Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase. J Cell Biol. 2013;201:249-61 pubmed publisher
    ..that the membrane-associated signaling protein phospholipase D (PLD) accumulates abnormally in cilia of Chlamydomonas reinhardtii bbs mutants...
  14. Schwarz C, Bohne A, Wang F, Cejudo F, Nickelsen J. An intermolecular disulfide-based light switch for chloroplast psbD gene expression in Chlamydomonas reinhardtii. Plant J. 2012;72:378-89 pubmed publisher
    ..In the green alga Chlamydomonas reinhardtii, D2 synthesis requires a high-molecular-weight complex containing the RNA stabilization factor Nac2 and ..
  15. Perreault F, Oukarroum A, Melegari S, Matias W, Popovic R. Polymer coating of copper oxide nanoparticles increases nanoparticles uptake and toxicity in the green alga Chlamydomonas reinhardtii. Chemosphere. 2012;87:1388-94 pubmed publisher
    ..In this study, we investigated the effect of polymer coating on CuO NP toxicity in the green alga Chlamydomonas reinhardtii by comparing bare and polymer-coated CuO NPs prepared from the same CuO nanopowder...
  16. Dal Molin C, Quek L, Palfreyman R, Nielsen L. AlgaGEM--a genome-scale metabolic reconstruction of algae based on the Chlamydomonas reinhardtii genome. BMC Genomics. 2011;12 Suppl 4:S5 pubmed publisher
  17. Blifernez Klassen O, Klassen V, Doebbe A, Kersting K, Grimm P, Wobbe L, et al. Cellulose degradation and assimilation by the unicellular phototrophic eukaryote Chlamydomonas reinhardtii. Nat Commun. 2012;3:1214 pubmed publisher
    ..Among phototrophs, the unicellular green microalga Chlamydomonas reinhardtii is widely known as one of the best established model organisms...
  18. Noth J, Krawietz D, Hemschemeier A, Happe T. Pyruvate:ferredoxin oxidoreductase is coupled to light-independent hydrogen production in Chlamydomonas reinhardtii. J Biol Chem. 2013;288:4368-77 pubmed publisher
    In anaerobiosis, the green alga Chlamydomonas reinhardtii evolves molecular hydrogen (H(2)) as one of several fermentation products...
  19. Niwa Y, Matsuo T, Onai K, Kato D, Tachikawa M, Ishiura M. Phase-resetting mechanism of the circadian clock in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2013;110:13666-71 pubmed publisher
    ..One of the major cues for this resetting mechanism is light. In the unicellular green alga Chlamydomonas reinhardtii, the circadian clock is reset by blue/green and red light...
  20. Dymek E, Smith E. PF19 encodes the p60 catalytic subunit of katanin and is required for assembly of the flagellar central apparatus in Chlamydomonas. J Cell Sci. 2012;125:3357-66 pubmed publisher
    ..In addition, we show that in Chlamydomonas the microtubule severing activity of p60 katanin is not required for stress-induced deflagellation or cell cycle progression as has been previously reported...
  21. Goduti D, Smith E. Analyses of functional domains within the PF6 protein of the central apparatus reveal a role for PF6 sub-complex members in regulating flagellar beat frequency. Cytoskeleton (Hoboken). 2012;69:179-94 pubmed publisher
    ..Analysis of double mutants lacking the amino terminus of PF6 and outer dynein arms reveal that C1a may play a role in modulating both inner and outer dynein arm activity...
  22. Tardif M, Atteia A, Specht M, Cogne G, Rolland N, Brugière S, et al. PredAlgo: a new subcellular localization prediction tool dedicated to green algae. Mol Biol Evol. 2012;29:3625-39 pubmed publisher
    The unicellular green alga Chlamydomonas reinhardtii is a prime model for deciphering processes occurring in the intracellular compartments of the photosynthetic cell...
  23. van Lis R, Baffert C, Coute Y, Nitschke W, Atteia A. Chlamydomonas reinhardtii chloroplasts contain a homodimeric pyruvate:ferredoxin oxidoreductase that functions with FDX1. Plant Physiol. 2013;161:57-71 pubmed publisher
    ..b>Chlamydomonas reinhardtii figures remarkably in this respect, because it efficiently produces hydrogen and its genome harbors many ..
  24. Liguori N, Roy L, Opacić M, Durand G, Croce R. Regulation of light harvesting in the green alga Chlamydomonas reinhardtii: the C-terminus of LHCSR is the knob of a dimmer switch. J Am Chem Soc. 2013;135:18339-42 pubmed publisher
    ..Here we demonstrate the unique ability of LHCSR, a stress-related LHC from the model organism Chlamydomonas reinhardtii, to sense pH variations, reversibly tuning its conformation from a light-harvesting state to a ..
  25. Esparza J, O TOOLE E, Li L, Giddings T, Kozak B, Albee A, et al. Katanin localization requires triplet microtubules in Chlamydomonas reinhardtii. PLoS ONE. 2013;8:e53940 pubmed publisher
    ..We suggest that the triplet microtubules are likely to play a key role in katanin p80 recruitment to the basal body of Chlamydomonas rather than the transition fibers that are needed for IFT localization...
  26. Zaffagnini M, Michelet L, Sciabolini C, Di Giacinto N, Morisse S, Marchand C, et al. High-resolution crystal structure and redox properties of chloroplastic triosephosphate isomerase from Chlamydomonas reinhardtii. Mol Plant. 2014;7:101-20 pubmed publisher
    ..Here, we have produced recombinantly and purified TPI from the unicellular green alga Chlamydomonas reinhardtii (Cr)...
  27. Kobayashi N, Noel E, Barnes A, Rosenberg J, DIRUSSO C, Black P, et al. Rapid detection and quantification of triacylglycerol by HPLC-ELSD in Chlamydomonas reinhardtii and Chlorella strains. Lipids. 2013;48:1035-49 pubmed publisher
    ..TAG accumulation in two Chlamydomonas reinhardtii (21 gr and CC503) and three Chlorella strains (UTEX 1230, CS01 and UTEX 2229) grown under conditions of ..
  28. Derrien B, Majeran W, Effantin G, Ebenezer J, Friso G, van Wijk K, et al. The purification of the Chlamydomonas reinhardtii chloroplast ClpP complex: additional subunits and structural features. Plant Mol Biol. 2012;80:189-202 pubmed publisher
    ..In this paper, we describe the purification of native chloroplast ClpP complex from the green alga Chlamydomonas reinhardtii, using a strain that carries the Strep-tag II at the C-terminus of the ClpP1 subunit...
  29. Gregory J, Li F, Tomosada L, Cox C, Topol A, Vinetz J, et al. Algae-produced Pfs25 elicits antibodies that inhibit malaria transmission. PLoS ONE. 2012;7:e37179 pubmed publisher
    ..falciparum sexual stage parasites and exhibit transmission blocking activity. Thus, algae are promising organisms for producing cysteine-disulfide-containing malaria transmission blocking vaccine candidate proteins...
  30. Brown J, DiPetrillo C, Smith E, Witman G. A FAP46 mutant provides new insights into the function and assembly of the C1d complex of the ciliary central apparatus. J Cell Sci. 2012;125:3904-13 pubmed publisher
    ..Here we report the isolation and characterization of a Chlamydomonas reinhardtii null mutant for FAP46...
  31. Page M, Allen M, Kropat J, Urzica E, Karpowicz S, Hsieh S, et al. Fe sparing and Fe recycling contribute to increased superoxide dismutase capacity in iron-starved Chlamydomonas reinhardtii. Plant Cell. 2012;24:2649-65 pubmed publisher
    ..Two pathways increase the capacity of the Chlamydomonas reinhardtii chloroplast to detoxify superoxide during Fe limitation stress...
  32. Liu B, Vieler A, Li C, Daniel Jones A, Benning C. Triacylglycerol profiling of microalgae Chlamydomonas reinhardtii and Nannochloropsis oceanica. Bioresour Technol. 2013;146:310-316 pubmed publisher
    ..TAG profiling was performed in Chlamydomonas reinhardtii and Nannochloropsis oceanica grown in nitrogen (N)-replete or N-depleted medium...
  33. Drop B, Webber Birungi M, Yadav S, Filipowicz Szymanska A, Fusetti F, Boekema E, et al. Light-harvesting complex II (LHCII) and its supramolecular organization in Chlamydomonas reinhardtii. Biochim Biophys Acta. 2014;1837:63-72 pubmed publisher
    ..Here we have analyzed the LHCII complex of the green alga Chlamydomonas reinhardtii and its association with the core of Photosystem II (PSII) to form multiprotein complexes...
  34. Ness R, Morgan A, Colegrave N, Keightley P. Estimate of the spontaneous mutation rate in Chlamydomonas reinhardtii. Genetics. 2012;192:1447-54 pubmed publisher
    ..rate and a description of the properties of new spontaneous mutations in the unicellular green alga Chlamydomonas reinhardtii. We conducted a mutation accumulation experiment for ?350 generations followed by whole-genome ..
  35. Ferrante P, Ballottari M, Bonente G, Giuliano G, Bassi R. LHCBM1 and LHCBM2/7 polypeptides, components of major LHCII complex, have distinct functional roles in photosynthetic antenna system of Chlamydomonas reinhardtii. J Biol Chem. 2012;287:16276-88 pubmed publisher
    The photosystem II antenna of Chlamydomonas reinhardtii is composed of monomeric and trimeric complexes, the latter encoded by LHCBM genes...
  36. Lucker B, Kramer D. Regulation of cyclic electron flow in Chlamydomonas reinhardtii under fluctuating carbon availability. Photosynth Res. 2013;117:449-59 pubmed publisher
    ..We assessed the kinetics and energy production of CEF activation in Chlamydomonas reinhardtii under rapid changes of organic and inorganic carbon availability...
  37. Li X, Moellering E, Liu B, Johnny C, Fedewa M, Sears B, et al. A galactoglycerolipid lipase is required for triacylglycerol accumulation and survival following nitrogen deprivation in Chlamydomonas reinhardtii. Plant Cell. 2012;24:4670-86 pubmed publisher
    ..phenomenon, we identified mutants with reduced TAG content following N deprivation in the model alga Chlamydomonas reinhardtii. In one of the mutants, the disruption of a galactoglycerolipid lipase-encoding gene, designated PLASTID ..
  38. Schein J, Hunt K, Minton J, Schultes N, Mourad G. The nucleobase cation symporter 1 of Chlamydomonas reinhardtii and that of the evolutionarily distant Arabidopsis thaliana display parallel function and establish a plant-specific solute transport profile. Plant Physiol Biochem. 2013;70:52-60 pubmed publisher
    The single cell alga Chlamydomonas reinhardtii is capable of importing purines as nitrogen sources. An analysis of the annotated C...
  39. Hoshino T, Johnson D, Cuello J. Design of new strategy for green algal photo-hydrogen production: spectral-selective photosystem I activation and photosystem II deactivation. Bioresour Technol. 2012;120:233-40 pubmed publisher
    ..Implementing the strategy by using a PSI light (692 nm peak, 680-700 nm) on Chlamydomonas reinhardtii cells resulted in relatively sustained photo-H(2) production (total of 0...
  40. Rasala B, Barrera D, Ng J, Plucinak T, Rosenberg J, Weeks D, et al. Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J. 2013;74:545-56 pubmed publisher
    ..mCerulean, green CrGFP, yellow Venus, orange tdTomato and red mCherry) in the popular model microalga Chlamydomonas reinhardtii. To circumvent the transgene silencing that often occurs in C...
  41. Johnson X, Alric J. Interaction between starch breakdown, acetate assimilation, and photosynthetic cyclic electron flow in Chlamydomonas reinhardtii. J Biol Chem. 2012;287:26445-52 pubmed publisher
    ..Here, using the unicellular microalga Chlamydomonas reinhardtii, we study the impact of heterotrophic/mixotrophic acetate feeding on chloroplast carbon metabolism by ..
  42. Mitchison H, Schmidts M, Loges N, Freshour J, Dritsoula A, Hirst R, et al. Mutations in axonemal dynein assembly factor DNAAF3 cause primary ciliary dyskinesia. Nat Genet. 2012;44:381-9, S1-2 pubmed publisher
    ..b>Chlamydomonas reinhardtii PF22 is exclusively cytoplasmic, and a PF22-null mutant cannot assemble any outer and some inner dynein ..
  43. Specht E, Mayfield S. Synthetic oligonucleotide libraries reveal novel regulatory elements in Chlamydomonas chloroplast mRNAs. ACS Synth Biol. 2013;2:34-46 pubmed publisher
    ..arrayed oligonucleotide library to examine in vivo hundreds of designed variants of endogenous UTRs from Chlamydomonas reinhardtii and quantitatively identify essential regions through next-generation sequencing of thousands of mutants...
  44. Chen L, Zhou L, Liu Y, Deng S, Wu H, Wang G. Toxicological effects of nanometer titanium dioxide (nano-TiO2) on Chlamydomonas reinhardtii. Ecotoxicol Environ Saf. 2012;84:155-62 pubmed publisher
    The toxicological effects of nanometer titanium dioxide (nano-TiO2) on a unicellular green alga Chlamydomonas reinhardtii were assessed by investigating the changes of the physiology and cyto-ultrastructure of this species under ..
  45. Amarnath K, Zaks J, Park S, Niyogi K, Fleming G. Fluorescence lifetime snapshots reveal two rapidly reversible mechanisms of photoprotection in live cells of Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2012;109:8405-10 pubmed publisher
    ..lifetimes responsible for the predominant, rapidly reversible component of NPQ, qE, in living cells of Chlamydomonas reinhardtii. Application of high light to dark-adapted cells of C...
  46. Pinto T, Malcata F, Arrabaça J, Silva J, Spreitzer R, Esquivel M. Rubisco mutants of Chlamydomonas reinhardtii enhance photosynthetic hydrogen production. Appl Microbiol Biotechnol. 2013;97:5635-43 pubmed publisher
    ..These results indicate that rubisco is a promising target for improving hydrogen production rates in engineered microalgae...
  47. Giardi M, Rea G, Lambreva M, Antonacci A, Pastorelli S, Bertalan I, et al. Mutations of photosystem II D1 protein that empower efficient phenotypes of Chlamydomonas reinhardtii under extreme environment in space. PLoS ONE. 2013;8:e64352 pubmed publisher
    ..we sent into low Earth orbit space engineered and mutated strains of the unicellular green alga, Chlamydomonas reinhardtii, which has been widely used as a model of photosynthetic organisms...
  48. Melegari S, Perreault F, Moukha S, Popovic R, Creppy E, Matias W. Induction to oxidative stress by saxitoxin investigated through lipid peroxidation in Neuro 2A cells and Chlamydomonas reinhardtii alga. Chemosphere. 2012;89:38-43 pubmed publisher
    ..4-3.0 nM) in two different cellular models: Neuro-2A (N2A) cells and Chlamydomonas reinhardtii alga by quantification of malondialdehyde (MDA) levels as indicative of lipid peroxidation (LPO)...
  49. Hung C, Ho M, Kanehara K, Nakamura Y. Functional study of diacylglycerol acyltransferase type 2 family in Chlamydomonas reinhardtii. FEBS Lett. 2013;587:2364-70 pubmed publisher
    ..DGTT1, 2 and 3 but not 4 complemented the phenotype, including triacylglycerol levels. Interestingly, complementation by DGTT2 increased triacylglycerol content by 9-fold. ..
  50. La Russa M, Bogen C, Uhmeyer A, Doebbe A, Filippone E, Kruse O, et al. Functional analysis of three type-2 DGAT homologue genes for triacylglycerol production in the green microalga Chlamydomonas reinhardtii. J Biotechnol. 2012;162:13-20 pubmed publisher
    ..DGAT homologue genes have been identified in the genome of the green alga Chlamydomonas reinhardtii, however their function in vivo is still unknown...
  51. Brueggeman A, Gangadharaiah D, Cserhati M, Casero D, Weeks D, Ladunga I. Activation of the carbon concentrating mechanism by CO2 deprivation coincides with massive transcriptional restructuring in Chlamydomonas reinhardtii. Plant Cell. 2012;24:1860-75 pubmed publisher
    ..network reconstruction to capture a remarkable scope of changes in gene expression that occurs when Chlamydomonas reinhardtii cells are shifted from high to very low levels of CO(2) (?100 ppm)...
  52. Johnson X, Alric J. Central carbon metabolism and electron transport in Chlamydomonas reinhardtii: metabolic constraints for carbon partitioning between oil and starch. Eukaryot Cell. 2013;12:776-93 pubmed publisher
    ..There are, however, constraints that govern central carbon metabolism in Chlamydomonas reinhardtii that are revealed by the compartmentalization and regulation of the pathways and their relation to key ..
  53. Allorent G, Tokutsu R, Roach T, Peers G, Cardol P, Girard Bascou J, et al. A dual strategy to cope with high light in Chlamydomonas reinhardtii. Plant Cell. 2013;25:545-57 pubmed publisher
    ..the respective roles of qE and qT in photoprotection, a mutant (npq4 stt7-9) was generated in Chlamydomonas reinhardtii by crossing the state transition-deficient mutant (stt7-9) with a strain having a largely reduced qE ..
  54. Fan J, Yan C, Andre C, Shanklin J, Schwender J, Xu C. Oil accumulation is controlled by carbon precursor supply for fatty acid synthesis in Chlamydomonas reinhardtii. Plant Cell Physiol. 2012;53:1380-90 pubmed publisher
    ..Using Chlamydomonas reinhardtii as a model system, we show here that starch, rather than oil, is the dominant storage sink for reduced ..
  55. Cakmak T, Angun P, Ozkan A, Cakmak Z, Olmez T, Tekinay T. Nitrogen and sulfur deprivation differentiate lipid accumulation targets of Chlamydomonas reinhardtii. Bioengineered. 2012;3:343-6 pubmed publisher
    ..1) (,) ( 2) We have previously demonstrated that N- and S-starved cells of Chlamydomonas reinhardtii display different metabolic trends, suggesting that different response mechanisms exist to compensate ..
  56. Yoo G, Park W, Kim C, Choi Y, Yang J. Direct lipid extraction from wet Chlamydomonas reinhardtii biomass using osmotic shock. Bioresour Technol. 2012;123:717-22 pubmed publisher
    ..Osmotic shock treatment was applied directly to wet Chlamydomonas reinhardtii biomass with water content >99%, along with both polar and non-polar organic solvents...
  57. Tevatia R, Demirel Y, Blum P. Kinetic modeling of photoautotropic growth and neutral lipid accumulation in terms of ammonium concentration in Chlamydomonas reinhardtii. Bioresour Technol. 2012;119:419-24 pubmed publisher
    This study focuses on the cell growth and the neutral lipid production modeling of Chlamydomonas reinhardtii in terms of different ammonium concentrations...
  58. Gregory J, Topol A, Doerner D, Mayfield S. Alga-produced cholera toxin-Pfs25 fusion proteins as oral vaccines. Appl Environ Microbiol. 2013;79:3917-25 pubmed publisher
    ..We engineered the chloroplast of the eukaryotic alga Chlamydomonas reinhardtii to produce a chimeric protein consisting of the 25-kDa Plasmodium falciparum surface protein (Pfs25) ..
  59. Kong F, Yamasaki T, Ohama T. Expression levels of domestic cDNA cassettes integrated in the nuclear genomes of various Chlamydomonas reinhardtii strains. J Biosci Bioeng. 2014;117:613-6 pubmed publisher
    ..Further improved Chlamydomonas strains are essential to facilitate high-throughput screening of transformants that express nuclear transgenes at a high level...
  60. Neupert J, Shao N, Lu Y, Bock R. Genetic transformation of the model green alga Chlamydomonas reinhardtii. Methods Mol Biol. 2012;847:35-47 pubmed publisher
    Over the past three decades, the single-celled green alga Chlamydomonas reinhardtii has become an invaluable model organism in plant biology and an attractive production host in biotechnology...
  61. Lin Y, Pan K, Hung C, Huang H, Chen C, Feng T, et al. Overexpression of ferredoxin, PETF, enhances tolerance to heat stress in Chlamydomonas reinhardtii. Int J Mol Sci. 2013;14:20913-29 pubmed publisher
    ..In this study, we demonstrate that overexpression of the endogenous photosynthetic FDX gene, PETF, in Chlamydomonas reinhardtii could raise the level of reduced ascorbate and diminish H2O2 levels under normal growth conditions...
  62. Jiang W, Rosenberg J, Wauchope A, Tremblay J, Shoemaker C, Weeks D, et al. Generation of a phage-display library of single-domain camelid VH H antibodies directed against Chlamydomonas reinhardtii antigens, and characterization of VH Hs binding cell-surface antigens. Plant J. 2013;76:709-17 pubmed publisher
    ..pacos) heavy chain-only variable VH domain (VH H) libraries against antigens in total cell lysates from Chlamydomonas reinhardtii. The sdAbs in the sera from immunized animals and VH H antibody domains isolated from the library show ..
  63. Urzica E, Adler L, Page M, Linster C, Arbing M, Casero D, et al. Impact of oxidative stress on ascorbate biosynthesis in Chlamydomonas via regulation of the VTC2 gene encoding a GDP-L-galactose phosphorylase. J Biol Chem. 2012;287:14234-45 pubmed publisher
    ..The Chlamydomonas reinhardtii genome encodes all the enzymes required for vitamin C biosynthesis via the L-galactose pathway...
  64. Kirst H, Garcia Cerdan J, Zurbriggen A, Ruehle T, Melis A. Truncated photosystem chlorophyll antenna size in the green microalga Chlamydomonas reinhardtii upon deletion of the TLA3-CpSRP43 gene. Plant Physiol. 2012;160:2251-60 pubmed publisher
    The truncated light-harvesting antenna size3 (tla3) DNA insertional transformant of Chlamydomonas reinhardtii is a chlorophyll-deficient mutant with a lighter green phenotype, a lower chlorophyll (Chl) per cell content, and higher Chl a/..
  65. Mathieu Rivet E, Scholz M, Arias C, Dardelle F, Schulze S, Le Mauff F, et al. Exploring the N-glycosylation pathway in Chlamydomonas reinhardtii unravels novel complex structures. Mol Cell Proteomics. 2013;12:3160-83 pubmed publisher
    b>Chlamydomonas reinhardtii is a green unicellular eukaryotic model organism for studying relevant biological and biotechnological questions. The availability of genomic resources and the growing interest in C...
  66. Belzile O, Hernandez Lara C, Wang Q, Snell W. Regulated membrane protein entry into flagella is facilitated by cytoplasmic microtubules and does not require IFT. Curr Biol. 2013;23:1460-5 pubmed publisher
    ..Thus, integral membrane proteins can negotiate passage through the ciliary diffusion barrier without the need for a motor. ..
  67. Fischer B, Ledford H, Wakao S, Huang S, Casero D, Pellegrini M, et al. SINGLET OXYGEN RESISTANT 1 links reactive electrophile signaling to singlet oxygen acclimation in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2012;109:E1302-11 pubmed publisher
    Acclimation of Chlamydomonas reinhardtii cells to low levels of singlet oxygen, produced either by photoreactive chemicals or high light treatment, induces a specific genetic response that strongly increases the tolerance of the algae to ..
  68. Roach T, Sedoud A, Krieger Liszkay A. Acetate in mixotrophic growth medium affects photosystem II in Chlamydomonas reinhardtii and protects against photoinhibition. Biochim Biophys Acta. 2013;1827:1183-90 pubmed publisher
    b>Chlamydomonas reinhardtii is a photoautotrophic green alga, which can be grown mixotrophically in acetate-supplemented media (Tris-acetate-phosphate). We show that acetate has a direct effect on photosystem II (PSII)...
  69. Santabarbara S, Casazza A, Ali K, Economou C, Wannathong T, Zito F, et al. The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii. Plant Physiol. 2013;161:535-46 pubmed publisher
    ..on the accumulation and function of the photosynthetic apparatus using a mutant of the green alga Chlamydomonas reinhardtii lacking carotenoids...
  70. Brzezowski P, Wilson K, Gray G. The PSBP2 protein of Chlamydomonas reinhardtii is required for singlet oxygen-dependent signaling. Planta. 2012;236:1289-303 pubmed
    In the green alga Chlamydomonas reinhardtii, the cytosolic Glutathione Peroxidase 5 gene (GPX5) is known to be transcriptionally up-regulated in response to singlet oxygen ((1)O(2))...
  71. Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, et al. Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell. 2012;24:4687-702 pubmed publisher
    The eyespot of Chlamydomonas reinhardtii is a light-sensitive organelle important for phototactic orientation of the alga. Here, we found that eyespot size is strain specific and downregulated in light...
  72. Malasarn D, Kropat J, Hsieh S, Finazzi G, Casero D, Loo J, et al. Zinc deficiency impacts CO2 assimilation and disrupts copper homeostasis in Chlamydomonas reinhardtii. J Biol Chem. 2013;288:10672-83 pubmed publisher
    ..We distinguished four nutritional zinc states in the alga Chlamydomonas reinhardtii: toxic, replete, deficient, and limited...
  73. Heuser T, Dymek E, Lin J, Smith E, Nicastro D. The CSC connects three major axonemal complexes involved in dynein regulation. Mol Biol Cell. 2012;23:3143-55 pubmed publisher
    ..Our results also indicate that although structurally very similar, RS1 and RS2 likely serve different functions in regulating flagellar motility...
  74. Rasala B, Lee P, Shen Z, Briggs S, Mendez M, Mayfield S. Robust expression and secretion of Xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide. PLoS ONE. 2012;7:e43349 pubmed publisher
    ..self-cleavage peptide to transcriptionally fuse heterologous gene expression to antibiotic resistance in Chlamydomonas reinhardtii. We demonstrate that strains transformed with ble-2A-GFP are zeocin-resistant and accumulate high levels ..
  75. Tran M, Henry R, Siefker D, Van C, Newkirk G, Kim J, et al. Production of anti-cancer immunotoxins in algae: ribosome inactivating proteins as fusion partners. Biotechnol Bioeng. 2013;110:2826-35 pubmed publisher
    The eukaryotic green algae, Chlamydomonas reinhardtii has been shown to be capable of producing a variety of recombinant proteins, but the true potential of this platform remains largely unexplored...
  76. Carbajal González B, Heuser T, Fu X, Lin J, Smith B, Mitchell D, et al. Conserved structural motifs in the central pair complex of eukaryotic flagella. Cytoskeleton (Hoboken). 2013;70:101-120 pubmed publisher
    ..These differences could be adaptations to the mechanical requirements of the rotating CPC in Chlamydomonas, compared to the Strongylocentrotus CPC which has a fixed orientation...
  77. Rügen M, Bockmayr A, Legrand J, Cogne G. Network reduction in metabolic pathway analysis: elucidation of the key pathways involved in the photoautotrophic growth of the green alga Chlamydomonas reinhardtii. Metab Eng. 2012;14:458-67 pubmed publisher
    ..We further analyze photoautotrophic metabolism of the green alga Chlamydomonas reinhardtii growing in a photobioreactor under maximal growth rate conditions...