chlamydomonas

Summary

Summary: A genus GREEN ALGAE in the order VOLVOCIDA. It consists of solitary biflagellated organisms common in fresh water and damp soil.

Top Publications

  1. Camargo A, Llamas A, Schnell R, Higuera J, Gonzalez Ballester D, Lefebvre P, et al. Nitrate signaling by the regulatory gene NIT2 in Chlamydomonas. Plant Cell. 2007;19:3491-503 pubmed
    Positive signaling by nitrate in its assimilation pathway has been studied in Chlamydomonas reinhardtii...
  2. Scholey J. Intraflagellar transport motors in cilia: moving along the cell's antenna. J Cell Biol. 2008;180:23-9 pubmed publisher
  3. Chochois V, Dauvillee D, Beyly A, Tolleter D, Cuiné S, Timpano H, et al. Hydrogen production in Chlamydomonas: photosystem II-dependent and -independent pathways differ in their requirement for starch metabolism. Plant Physiol. 2009;151:631-40 pubmed publisher
    Under sulfur deprivation conditions, the green alga Chlamydomonas reinhardtii produces hydrogen in the light in a sustainable manner thanks to the contribution of two pathways, direct and indirect...
  4. Liu Z, Takazaki H, Nakazawa Y, Sakato M, Yagi T, Yasunaga T, et al. Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain. Eukaryot Cell. 2008;7:1136-45 pubmed publisher
    The outer dynein arm of Chlamydomonas flagella contains three heavy chains (alpha, beta, and gamma), each of which exhibits motor activity. How they assemble and cooperate is of considerable interest...
  5. Liu Y, Misamore M, Snell W. Membrane fusion triggers rapid degradation of two gamete-specific, fusion-essential proteins in a membrane block to polygamy in Chlamydomonas. Development. 2010;137:1473-81 pubmed publisher
    ..During fertilization in the green alga Chlamydomonas, the plus gamete-specific membrane protein FUS1 is required for adhesion between the apically localized sites ..
  6. Casas Mollano J, Jeong B, Xu J, Moriyama H, Cerutti H. The MUT9p kinase phosphorylates histone H3 threonine 3 and is necessary for heritable epigenetic silencing in Chlamydomonas. Proc Natl Acad Sci U S A. 2008;105:6486-91 pubmed publisher
    ..We report here that a Chlamydomonas reinhardtii mutant defective in a Ser/Thr protein kinase (MUT9p), which phosphorylates histones H3 and H2A, ..
  7. Mueller J, Perrone C, Bower R, Cole D, Porter M. The FLA3 KAP subunit is required for localization of kinesin-2 to the site of flagellar assembly and processive anterograde intraflagellar transport. Mol Biol Cell. 2005;16:1341-54 pubmed
    ..with the IFT particle or how their activities might be coordinated, we characterized a ts mutation in the Chlamydomonas gene encoding KAP, the nonmotor subunit of Kinesin-2...
  8. Szyszka B, Ivanov A, Huner N. Psychrophily is associated with differential energy partitioning, photosystem stoichiometry and polypeptide phosphorylation in Chlamydomonas raudensis. Biochim Biophys Acta. 2007;1767:789-800 pubmed
    b>Chlamydomonas raudensis UWO 241 and SAG 49.72 represent the psychrophilic and mesophilic strains of this green algal species...
  9. Keller L, Romijn E, Zamora I, Yates J, Marshall W. Proteomic analysis of isolated chlamydomonas centrioles reveals orthologs of ciliary-disease genes. Curr Biol. 2005;15:1090-8 pubmed
    ..technology) to identify the protein composition of basal bodies (centrioles) isolated from the green alga Chlamydomonas reinhardtii...

More Information

Publications97

  1. Parker J, Hilton L, Diener D, Rasi M, Mahjoub M, Rosenbaum J, et al. Centrioles are freed from cilia by severing prior to mitosis. Cytoskeleton (Hoboken). 2010;67:425-30 pubmed publisher
    ..Here we show by live cell imaging and immunofluorescence microscopy that resorbing flagella of Chlamydomonas leave remnants associated with the mother cell wall...
  2. Nordhues A, Miller S, Mühlhaus T, Schroda M. New insights into the roles of molecular chaperones in Chlamydomonas and Volvox. Int Rev Cell Mol Biol. 2010;285:75-113 pubmed publisher
    The unicellular green alga Chlamydomonas reinhardtii has been used as a model organism for many decades, mainly to study photosynthesis and flagella/cilia...
  3. Mahjoub M, Qasim Rasi M, Quarmby L. A NIMA-related kinase, Fa2p, localizes to a novel site in the proximal cilia of Chlamydomonas and mouse kidney cells. Mol Biol Cell. 2004;15:5172-86 pubmed
    ..We previously showed that the Nek Fa2p is important for ciliary function and cell cycle in Chlamydomonas. We now show that Fa2p localizes to an important regulatory site at the proximal end of cilia in both ..
  4. Wong J, Johnson M. Is HAP2-GCS1 an ancestral gamete fusogen?. Trends Cell Biol. 2010;20:134-41 pubmed publisher
    ..We propose that HAP2-GCS1 is a crucial component of an ancient mechanism that mediates the fusion of gamete plasma membranes and could have been a key early innovation in the evolution of sexual reproduction. ..
  5. Snell W, Pan J, Wang Q. Cilia and flagella revealed: from flagellar assembly in Chlamydomonas to human obesity disorders. Cell. 2004;117:693-7 pubmed
    The recent identification in Chlamydomonas of the intraflagellar transport machinery that assembles cilia and flagella has triggered a renaissance of interest in these organelles that transcends studies on their well-characterized ..
  6. Wirschell M, Hendrickson T, Sale W. Keeping an eye on I1: I1 dynein as a model for flagellar dynein assembly and regulation. Cell Motil Cytoskeleton. 2007;64:569-79 pubmed
    ..Diverse studies, particularly in Chlamydomonas, have determined that the inner arm dynein I1 is targeted to a unique structural position and is critical for ..
  7. Kramzar L, Mueller T, Erickson B, Higgs D. Regulatory sequences of orthologous petD chloroplast mRNAs are highly specific among Chlamydomonas species. Plant Mol Biol. 2006;60:405-22 pubmed publisher
    ..The petD chloroplast mRNA in Chlamydomonas reinhardtii has three such essential regulatory elements in its 362-nt long 5' UTR...
  8. Oiwa K, Sakakibara H. Recent progress in dynein structure and mechanism. Curr Opin Cell Biol. 2005;17:98-103 pubmed
    ..Recent progress sheds light on key unsolved questions concerning how the molecule is really organized, what conformational changes accompany ATP hydrolysis and whether two or three motor domains are coordinated in their motion. ..
  9. Dentler W. A role for the membrane in regulating Chlamydomonas flagellar length. PLoS ONE. 2013;8:e53366 pubmed publisher
    ..Fully grown steady-state Chlamydomonas flagella release flagellar vesicles from their tips and failure to resupply membrane should affect flagellar ..
  10. von Gromoff E, Alawady A, Meinecke L, Grimm B, Beck C. Heme, a plastid-derived regulator of nuclear gene expression in Chlamydomonas. Plant Cell. 2008;20:552-67 pubmed publisher
    To gain insight into the chloroplast-to-nucleus signaling role of tetrapyrroles, Chlamydomonas reinhardtii mutants in the Mg-chelatase that catalyzes the insertion of magnesium into protoporphyrin IX were isolated and characterized...
  11. Houille Vernes L, Rappaport F, Wollman F, Alric J, Johnson X. Plastid terminal oxidase 2 (PTOX2) is the major oxidase involved in chlororespiration in Chlamydomonas. Proc Natl Acad Sci U S A. 2011;108:20820-5 pubmed publisher
    ..the unique plastid terminal oxidase (PTOX) found in plants, two genes encoding oxidases have been found in the Chlamydomonas genome, PTOX1 and PTOX2. Here we report the identification of a knockout mutant of PTOX2...
  12. Spijkerman E, Wacker A. Interactions between P-limitation and different C conditions on the fatty acid composition of an extremophile microalga. Extremophiles. 2011;15:597-609 pubmed publisher
    The extremophilic microalga Chlamydomonas acidophila inhabits very acidic waters (pH 2-3.5), where its growth is often limited by phosphorus (P) or colimited by P and inorganic carbon (CO(2))...
  13. Li Y, Han D, Hu G, Dauvillee D, Sommerfeld M, Ball S, et al. Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol. Metab Eng. 2010;12:387-91 pubmed publisher
    ..We demonstrate that inactivation of ADP-glucose pyrophosphorylase in a Chlamydomonas starchless mutant led to a 10-fold increase in TAG, suggesting that shunting of photosynthetic carbon ..
  14. Tomita H, Sugano E, Yawo H, Ishizuka T, Isago H, Narikawa S, et al. Restoration of visual response in aged dystrophic RCS rats using AAV-mediated channelopsin-2 gene transfer. Invest Ophthalmol Vis Sci. 2007;48:3821-6 pubmed
    ..These results suggest that the transduction of Chop2 would provide a new strategy to treat some retinitis pigmentosa (RP) symptoms independent of their etiology. ..
  15. Yagi T. Bioinformatic approaches to dynein heavy chain classification. Methods Cell Biol. 2009;92:1-9 pubmed publisher
    ..The Chlamydomonas genome contains 16 DHC genes, of which 15 genes have been correlated with particular DHC proteins...
  16. King S. Purification of axonemal dyneins and dynein-associated components from Chlamydomonas. Methods Cell Biol. 2009;92:31-48 pubmed publisher
    ..The dyneins from flagella of Chlamydomonas have been extensively studied as this organism is amenable to genetic, biochemical, and physiological ..
  17. Zimmer S, Schein A, Zipor G, Stern D, Schuster G. Polyadenylation in Arabidopsis and Chlamydomonas organelles: the input of nucleotidyltransferases, poly(A) polymerases and polynucleotide phosphorylase. Plant J. 2009;59:88-99 pubmed publisher
    ..In a search for the enzymes responsible for adding poly(A) tails in Chlamydomonas and Arabidopsis organelles, reverse genetic and biochemical approaches were employed...
  18. Pedersen L, Rompolas P, Christensen S, Rosenbaum J, King S. The lissencephaly protein Lis1 is present in motile mammalian cilia and requires outer arm dynein for targeting to Chlamydomonas flagella. J Cell Sci. 2007;120:858-67 pubmed
    ..Moreover, a NudC ortholog is upregulated upon deflagellation in Chlamydomonas. We found that mammalian Lis1 localizes to motile cilia in trachea and oviduct, but is absent from non-motile ..
  19. Pigino G, Maheshwari A, Bui K, Shingyoji C, Kamimura S, Ishikawa T. Comparative structural analysis of eukaryotic flagella and cilia from Chlamydomonas, Tetrahymena, and sea urchins. J Struct Biol. 2012;178:199-206 pubmed publisher
    ..cilia, and compare it with the axoneme of the flagellum of sea urchin sperm, as well as with the axoneme of Chlamydomonas flagella, which we analyzed previously...
  20. Bradley B, Wagner J, Quarmby L. Identification and sequence analysis of six new members of the NIMA-related kinase family in Chlamydomonas. J Eukaryot Microbiol. 2004;51:66-72 pubmed
    ..We report six new members of this family in Chlamydomonas, in addition to the previously identified NIMA-related kinase, Fa2p...
  21. Salinas T, Duby F, Larosa V, Coosemans N, Bonnefoy N, Motte P, et al. Co-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas. PLoS Genet. 2012;8:e1002946 pubmed publisher
    ..In the unicellular green alga Chlamydomonas, a precise correlation was found between the mitochondrial codon usage and the nature and amount of imported ..
  22. Kropat J, Tottey S, Birkenbihl R, Depège N, Huijser P, Merchant S. A regulator of nutritional copper signaling in Chlamydomonas is an SBP domain protein that recognizes the GTAC core of copper response element. Proc Natl Acad Sci U S A. 2005;102:18730-5 pubmed
    ..locus, required for both activating and repressing target genes of a copper- and hypoxia-sensing pathway in Chlamydomonas, encodes a 1,232-residue candidate transcription factor with a plant-specific DNA-binding domain named SBP, ..
  23. Goldschmidt Clermont M, Rahire M, Rochaix J. Redundant cis-acting determinants of 3' processing and RNA stability in the chloroplast rbcL mRNA of Chlamydomonas. Plant J. 2008;53:566-77 pubmed publisher
    We have designed a screen for mutants affected in 3' maturation of the chloroplast rbcL mRNA in Chlamydomonas reinhardtii...
  24. Iomini C, Tejada K, Mo W, Vaananen H, Piperno G. Primary cilia of human endothelial cells disassemble under laminar shear stress. J Cell Biol. 2004;164:811-7 pubmed
    ..gene-1 product (CMG-1), a human homologue of the intraflagellar transport (IFT) protein IFT-71 in Chlamydomonas. CMG-1 was present in particles along primary cilia of HUVEC at interphase and around the oldest basal body/..
  25. Dauvillee D, Delhaye S, Gruyer S, Slomianny C, Moretz S, D Hulst C, et al. Engineering the chloroplast targeted malarial vaccine antigens in Chlamydomonas starch granules. PLoS ONE. 2010;5:e15424 pubmed publisher
    ..GBSS), the major protein associated to the starch matrix in all starch-accumulating plants and algae such as Chlamydomonas reinhardtii...
  26. Takizawa K, Takahashi S, Huner N, Minagawa J. Salinity affects the photoacclimation of Chlamydomonas raudensis Ettl UWO241. Photosynth Res. 2009;99:195-203 pubmed publisher
    b>Chlamydomonas raudensis Ettl UWO241, a natural variant of C. raudensis, is deficient in state transitions. Its habitat, the deepest layer of Lake Bonney in Antarctica, features low irradiance, low temperature, and high salinity...
  27. Kothari R, Prasad R, Kumar V, Singh D. Production of biodiesel from microalgae Chlamydomonas polypyrenoideum grown on dairy industry wastewater. Bioresour Technol. 2013;144:499-503 pubmed publisher
    This study involves a process of phyco-remediation of dairy industry wastewater by algal strain Chlamydomonas polypyrenoideum...
  28. Casey D, Yagi T, Kamiya R, Witman G. DC3, the smallest subunit of the Chlamydomonas flagellar outer dynein arm-docking complex, is a redox-sensitive calcium-binding protein. J Biol Chem. 2003;278:42652-9 pubmed
    ..The Chlamydomonas ODA-DC contains three proteins; loss of any one prevents normal assembly of the outer arm, leading to a slow, ..
  29. Dentler W, VanderWaal K, Porter M. Recording and analyzing IFT in Chlamydomonas flagella. Methods Cell Biol. 2009;93:145-55 pubmed publisher
    ..The focus of this chapter is to provide methods to observe and quantify the movements of IFT particles in Chlamydomonas flagella...
  30. Fernandez E, Galvan A. Nitrate assimilation in Chlamydomonas. Eukaryot Cell. 2008;7:555-9 pubmed publisher
  31. King S. Axonemal protofilament ribbons, DM10 domains, and the link to juvenile myoclonic epilepsy. Cell Motil Cytoskeleton. 2006;63:245-53 pubmed
    ..In Chlamydomonas, a protein (Rib72) of almost identical domain structure is a component of the protofilament ribbons within the ..
  32. Hummel E, Schmickl R, Hinz G, Hillmer S, Robinson D. Brefeldin A action and recovery in Chlamydomonas are rapid and involve fusion and fission of Golgi cisternae. Plant Biol (Stuttg). 2007;9:489-501 pubmed
    b>CHLAMYDOMONAS NOCTIGAMA has a non-motile Golgi apparatus consisting of several Golgi stacks adjacent to transitional ER...
  33. Falciatore A, Merendino L, Barneche F, Ceol M, Meskauskiene R, Apel K, et al. The FLP proteins act as regulators of chlorophyll synthesis in response to light and plastid signals in Chlamydomonas. Genes Dev. 2005;19:176-87 pubmed
    ..We have examined the function of a Flu-like gene (FLP) from Chlamydomonas that gives rise to two FLP transcripts through alternative splicing...
  34. Arisz S, Munnik T. The salt stress-induced LPA response in Chlamydomonas is produced via PLA? hydrolysis of DGK-generated phosphatidic acid. J Lipid Res. 2011;52:2012-20 pubmed publisher
    The unicellular green alga Chlamydomonas has frequently been used as a eukaryotic model system to study intracellular phospholipid signaling pathways in response to environmental stresses...
  35. McCord R, Yukich J, Bernd K. Analysis of force generation during flagellar assembly through optical trapping of free-swimming Chlamydomonas reinhardtii. Cell Motil Cytoskeleton. 2005;61:137-44 pubmed
    ..models to investigate the establishment, maintenance, and function of flagella of the biflagellate green algae Chlamydomonas reinhardtii. We report the first direct measurement of Chlamydomonas flagellar swimming force...
  36. Lodha M, Schroda M. Analysis of chromatin structure in the control regions of the chlamydomonas HSP70A and RBCS2 genes. Plant Mol Biol. 2005;59:501-13 pubmed
    ..micrococcal nuclease sensitivity assays to determine the chromatin structures in the control regions of the Chlamydomonas reinhardtii HSP70A and RBCS2 genes...
  37. Olson B, Oberholzer M, Li Y, Zones J, Kohli H, Bisova K, et al. Regulation of the Chlamydomonas cell cycle by a stable, chromatin-associated retinoblastoma tumor suppressor complex. Plant Cell. 2010;22:3331-47 pubmed publisher
    We examined the cell cycle dynamics of the retinoblastoma (RB) protein complex in the unicellular alga Chlamydomonas reinhardtii that has single homologs for each subunit-RB, E2F, and DP...
  38. Lindemann C, Mitchell D. Evidence for axonemal distortion during the flagellar beat of Chlamydomonas. Cell Motil Cytoskeleton. 2007;64:580-9 pubmed
    ..The rapid fixation method employed by Mitchell was able to preserve the waveform of Chlamydomonas flagella much as it appears during normal flagellar beating [Mitchell, Cell Motil Cytoskeleton 2003;56:120-129]..
  39. Nicastro D, Fu X, Heuser T, Tso A, Porter M, Linck R. Cryo-electron tomography reveals conserved features of doublet microtubules in flagella. Proc Natl Acad Sci U S A. 2011;108:E845-53 pubmed publisher
    ..We have used cryo-electron tomography of Chlamydomonas and sea urchin flagella to answer long-standing questions and to provide information about the structure of ..
  40. Koblenz B, Schoppmeier J, Grunow A, Lechtreck K. Centrin deficiency in Chlamydomonas causes defects in basal body replication, segregation and maturation. J Cell Sci. 2003;116:2635-46 pubmed
    ..A construct inducing centrin RNAi was used to study the effect of centrin deficiency in Chlamydomonas. Transformants contained variable amounts of residual centrin (down to 5% of wild-type) and lacked centrin ..
  41. Pan J, Wang Q, Snell W. An aurora kinase is essential for flagellar disassembly in Chlamydomonas. Dev Cell. 2004;6:445-51 pubmed
    ..Here, we show that the aurora protein kinase CALK in the biflagellated alga Chlamydomonas has a central role in two pathways for eliminating flagella...
  42. Jans F, Mignolet E, Houyoux P, Cardol P, Ghysels B, Cuiné S, et al. A type II NAD(P)H dehydrogenase mediates light-independent plastoquinone reduction in the chloroplast of Chlamydomonas. Proc Natl Acad Sci U S A. 2008;105:20546-51 pubmed publisher
    ..approach that the NDA2 gene, belonging to a type II NAD(P)H dehydrogenases family in the green microalga Chlamydomonas reinhardtii, encodes a chloroplastic dehydrogenase that functions to reduce PQ nonphotochemically in this alga...
  43. Nordhues A, Schöttler M, Unger A, Geimer S, Schönfelder S, Schmollinger S, et al. Evidence for a role of VIPP1 in the structural organization of the photosynthetic apparatus in Chlamydomonas. Plant Cell. 2012;24:637-59 pubmed publisher
    ..As this functional assignment is under debate, we investigated the function of VIPP1 in Chlamydomonas reinhardtii. Using immunofluorescence, we localized VIPP1 to distinct spots within the chloroplast...
  44. Wilson N. Gametic cell adhesion and fusion in the unicellular alga Chlamydomonas. Methods Mol Biol. 2008;475:39-51 pubmed publisher
    Differentiation of vegetative cells of the haploid eukaryote Chlamydomonas is dependent on environmental conditions...
  45. Odom O, Shenkenberg D, Garcia J, Herrin D. A horizontally acquired group II intron in the chloroplast psbA gene of a psychrophilic Chlamydomonas: in vitro self-splicing and genetic evidence for maturase activity. RNA. 2004;10:1097-107 pubmed
    ..Herein, we describe a large (2.6-kb) group II intron from the psbA gene (psbA1) of a psychrophilic Chlamydomonas sp. from Antarctica that self-splices accurately in vitro...
  46. Ushimaru Y, Konno A, Kaizu M, Ogawa K, Satoh N, Inaba K. Association of a 66 kDa homolog of Chlamydomonas DC2, a subunit of the outer arm docking complex, with outer arm dynein of sperm flagella in the ascidian Ciona intestinalis. Zoolog Sci. 2006;23:679-87 pubmed
    ..Here we found that Ci-Axp66.0 shows sequence similarity to the DC2 subunit of the Chlamydomonas outer arm docking complex. Analysis of secondary structure of Ci-Axp66...
  47. Peers G, Niyogi K. Pond scum genomics: the genomes of Chlamydomonas and Ostreococcus. Plant Cell. 2008;20:502-7 pubmed publisher
  48. Pocock T, Vetterli A, Falk S. Evidence for phenotypic plasticity in the Antarctic extremophile Chlamydomonas raudensis Ettl. UWO 241. J Exp Bot. 2011;62:1169-77 pubmed publisher
    ..The Antarctic extremophile Chlamydomonas raudensis Ettl...
  49. Toba S, Fox L, Sakakibara H, Porter M, Oiwa K, Sale W. Distinct roles of 1alpha and 1beta heavy chains of the inner arm dynein I1 of Chlamydomonas flagella. Mol Biol Cell. 2011;22:342-53 pubmed publisher
    The Chlamydomonas I1 dynein is a two-headed inner dynein arm important for the regulation of flagellar bending...
  50. Perrone C, Tritschler D, Taulman P, Bower R, Yoder B, Porter M. A novel dynein light intermediate chain colocalizes with the retrograde motor for intraflagellar transport at sites of axoneme assembly in chlamydomonas and Mammalian cells. Mol Biol Cell. 2003;14:2041-56 pubmed
    ..To see whether the LIC might play a role in IFT, we characterized the gene encoding the Chlamydomonas homolog of D2LIC and found its expression is up-regulated in response to deflagellation...
  51. Hua J, Smith D, Borza T, Lee R. Similar relative mutation rates in the three genetic compartments of Mesostigma and Chlamydomonas. Protist. 2012;163:105-15 pubmed publisher
    ..algae, as substitution rate data is limited to only the mitochondrial and nuclear genomes of the chlorophyte Chlamydomonas. Here, we measure silent-site substitution rates in the plastid DNA of Chlamydomonas and the three genetic ..
  52. Luo M, Cao M, Kan Y, Li G, Snell W, Pan J. The phosphorylation state of an aurora-like kinase marks the length of growing flagella in Chlamydomonas. Curr Biol. 2011;21:586-91 pubmed publisher
    ..Previously, we showed that the phosphorylation state of the aurora-like protein kinase CALK in Chlamydomonas is a marker of the absence of flagella...
  53. Wood C, Wang Z, Diener D, Zones J, Rosenbaum J, Umen J. IFT proteins accumulate during cell division and localize to the cleavage furrow in Chlamydomonas. PLoS ONE. 2012;7:e30729 pubmed publisher
    ..Here, we used synchronous cultures of Chlamydomonas to investigate the temporal patterns of accumulation and localization of IFT proteins during the cell cycle...
  54. Furuta A, Yagi T, Yanagisawa H, Higuchi H, Kamiya R. Systematic comparison of in vitro motile properties between Chlamydomonas wild-type and mutant outer arm dyneins each lacking one of the three heavy chains. J Biol Chem. 2009;284:5927-35 pubmed publisher
    ..To elucidate their functional difference, we compared the in vitro motile properties of Chlamydomonas wild-type OAD containing the alpha, beta, and gamma heavy chains and three kinds of mutant OADs, each lacking ..
  55. Eberhard S, Finazzi G, Wollman F. The dynamics of photosynthesis. Annu Rev Genet. 2008;42:463-515 pubmed publisher
  56. Liu Y, Tewari R, Ning J, Blagborough A, Garbom S, Pei J, et al. The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes. Genes Dev. 2008;22:1051-68 pubmed publisher
    ..Here, by use of gene discovery methods in the green alga Chlamydomonas, gene disruption in the rodent malaria parasite Plasmodium berghei, and distinctive features of fertilization ..
  57. Gerloff Elias A, Barua D, Mölich A, Spijkerman E. Temperature- and pH-dependent accumulation of heat-shock proteins in the acidophilic green alga Chlamydomonas acidophila. FEMS Microbiol Ecol. 2006;56:345-54 pubmed
    b>Chlamydomonas acidophila, a unicellular green alga, is a dominant phytoplankton species in acidic water bodies, facing severe environmental conditions such as low pH and high heavy metal concentrations...
  58. Li J, Gerdes J, Haycraft C, Fan Y, Teslovich T, May Simera H, et al. Comparative genomics identifies a flagellar and basal body proteome that includes the BBS5 human disease gene. Cell. 2004;117:541-52 pubmed
    ..the nonflagellated proteome of Arabidopsis from the shared proteome of the ciliated/flagellated organisms Chlamydomonas and human...
  59. Piao T, Luo M, Wang L, Guo Y, Li D, Li P, et al. A microtubule depolymerizing kinesin functions during both flagellar disassembly and flagellar assembly in Chlamydomonas. Proc Natl Acad Sci U S A. 2009;106:4713-8 pubmed publisher
    ..Here, we show that Chlamydomonas kinesin-13 (CrKinesin-13), a member of the kinesin-13 family of microtubule depolymerizing kinesins best known ..
  60. Miller M, Esparza J, Lippa A, Lux F, Cole D, Dutcher S. Mutant kinesin-2 motor subunits increase chromosome loss. Mol Biol Cell. 2005;16:3810-20 pubmed
    The Chlamydomonas anterograde intraflagellar transport motor, kinesin-2, is isolated as a heterotrimeric complex containing two motor subunits and a nonmotor subunit known as kinesin-associated polypeptide or KAP...
  61. Gonzalez Ballester D, Camargo A, Fernandez E. Ammonium transporter genes in Chlamydomonas: the nitrate-specific regulatory gene Nit2 is involved in Amt1;1 expression. Plant Mol Biol. 2004;56:863-78 pubmed
    Ammonium transport is a key process in nitrogen metabolism. In the green alga Chlamydomonas, we have characterized molecularly the largest family of ammonium transporters (AMT1) so far described consisting of eight members...
  62. Spijkerman E. The expression of a carbon concentrating mechanism in Chlamydomonas acidophila under variable phosphorus, iron, and CO2 concentrations. Photosynth Res. 2011;109:179-89 pubmed publisher
    The CO(2) acquisition was analyzed in Chlamydomonas acidophila at pH 2.4 in a range of medium P and Fe concentrations and at high and low CO(2) condition...
  63. Pedersen L, Geimer S, Rosenbaum J. Dissecting the molecular mechanisms of intraflagellar transport in chlamydomonas. Curr Biol. 2006;16:450-9 pubmed
    ..Most of the components of the IFT system have been identified and characterized, but the mechanisms by which these different components are coordinated and regulated at the flagellar base and tip are unclear...
  64. Scholey J, Anderson K. Intraflagellar transport and cilium-based signaling. Cell. 2006;125:439-42 pubmed
    ..A new study (Wang et al., 2006) illuminates the role of IFT in cilium-based signaling during mating in the alga Chlamydomonas.
  65. Marshall W, Qin H, Rodrigo Brenni M, Rosenbaum J. Flagellar length control system: testing a simple model based on intraflagellar transport and turnover. Mol Biol Cell. 2005;16:270-8 pubmed
    ..These results are consistent with the balance-point model for length control. The three strategies used here are not limited to flagella and can in principle be adapted to probe size control systems for any organelle...
  66. Lapaille M, Thiry M, Perez E, Gonzalez Halphen D, Remacle C, Cardol P. Loss of mitochondrial ATP synthase subunit beta (Atp2) alters mitochondrial and chloroplastic function and morphology in Chlamydomonas. Biochim Biophys Acta. 2010;1797:1533-9 pubmed publisher
    ..by RNA interference the expression of the ATP2 gene, coding for the catalytic subunit beta, in the green alga Chlamydomonas reinhardtii...
  67. Tam L, Dentler W, Lefebvre P. Defective flagellar assembly and length regulation in LF3 null mutants in Chlamydomonas. J Cell Biol. 2003;163:597-607 pubmed
    Four long-flagella (LF) genes are important for flagellar length control in Chlamydomonas reinhardtii. Here, we characterize two new null lf3 mutants whose phenotypes are different from previously identified lf3 mutants...
  68. Spijkerman E. What physiological acclimation supports increased growth at high CO2 conditions?. Physiol Plant. 2008;133:41-8 pubmed publisher
    b>Chlamydomonas acidophila Negoro is a green algal species abundant in acidic waters (pH 2-3.5), in which inorganic carbon is present only as CO(2)...
  69. Meyer M, Genkov T, Skepper J, Jouhet J, Mitchell M, Spreitzer R, et al. Rubisco small-subunit ?-helices control pyrenoid formation in Chlamydomonas. Proc Natl Acad Sci U S A. 2012;109:19474-9 pubmed publisher
    ..Using the model alga Chlamydomonas reinhardtii, we show that pyrenoid formation, Rubisco aggregation, and CCM activity relate to discrete regions ..
  70. Rupp G, Porter M. A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest-specific gene product. J Cell Biol. 2003;162:47-57 pubmed
    ..To identify subunits of the DRC, we characterized a Chlamydomonas motility mutant obtained by insertional mutagenesis...
  71. Collins S, de Meaux J. Adaptation to different rates of environmental change in Chlamydomonas. Evolution. 2009;63:2952-65 pubmed publisher
    We investigate how different rates of environmental change affect adaptive outcomes and dynamics by selecting Chlamydomonas populations for over 200 generations in environments where the rate of change varies...
  72. Poerschmann J, Spijkerman E, Langer U. Fatty acid patterns in Chlamydomonas sp. as a marker for nutritional regimes and temperature under extremely acidic conditions. Microb Ecol. 2004;48:78-89 pubmed
    Fatty acid profiles were used to characterize nutritional pathways in Chlamydomonas sp. isolated from an acidic mining lake (pH 2.7). Surprisingly, profiles of Chlamydomonas sp...
  73. Hou S, Govorunova E, Ntefidou M, Lane C, Spudich E, Sineshchekov O, et al. Diversity of Chlamydomonas channelrhodopsins. Photochem Photobiol. 2012;88:119-28 pubmed publisher
    ..The first two channelrhodopsins were obtained from the model organism Chlamydomonas reinhardtii (CrChR1 and CrChR2)...
  74. Uniacke J, Zerges W. Chloroplast protein targeting involves localized translation in Chlamydomonas. Proc Natl Acad Sci U S A. 2009;106:1439-44 pubmed publisher
    ..confocal microscopy to explore the targeting mechanisms used by several chloroplast proteins in the green alga Chlamydomonas. These include the small subunit of ribulose bisphosphate carboxylase (rubisco) and the light-harvesting ..
  75. Ahmed N, Mitchell D. ODA16p, a Chlamydomonas flagellar protein needed for dynein assembly. Mol Biol Cell. 2005;16:5004-12 pubmed
    ..To understand how dyneins assemble and attach to this network, we characterized two Chlamydomonas outer arm dynein assembly (oda) mutants at a new locus, ODA16...
  76. Grossman A, Croft M, Gladyshev V, Merchant S, Posewitz M, Prochnik S, et al. Novel metabolism in Chlamydomonas through the lens of genomics. Curr Opin Plant Biol. 2007;10:190-8 pubmed
    b>Chlamydomonas has traditionally been exploited as an organism that is associated with sophisticated physiological, genetic and molecular analyses, all of which have been used to elucidate several biological processes, especially ..
  77. Immeln D, Schlesinger R, Heberle J, Kottke T. Blue light induces radical formation and autophosphorylation in the light-sensitive domain of Chlamydomonas cryptochrome. J Biol Chem. 2007;282:21720-8 pubmed
    ..In the genome of the unicellular green alga Chlamydomonas reinhardtii, a single plant cryptochrome, Chlamydomonas photolyase homologue 1 (CPH1), has been identified...
  78. Fang S, Umen J. A suppressor screen in chlamydomonas identifies novel components of the retinoblastoma tumor suppressor pathway. Genetics. 2008;178:1295-310 pubmed publisher
    The retinoblastoma (RB) protein is a eukaryotic tumor suppressor and negative cell-cycle regulator. Chlamydomonas reinhardtii cells that lack the RB homolog MAT3 show loss of size checkpoint control and deregulated cell-cycle progression ..
  79. Steele R, Dana C. Evolutionary history of the HAP2/GCS1 gene and sexual reproduction in metazoans. PLoS ONE. 2009;4:e7680 pubmed publisher
    ..and protists, and is required in the male gamete for fusion to the female gamete in the unicellular organisms Chlamydomonas and Plasmodium...
  80. Wang B, Liu C, Wu Y. Effect of heavy metals on the activity of external carbonic anhydrase of microalga Chlamydomonas reinhardtii and microalgae from karst lakes. Bull Environ Contam Toxicol. 2005;74:227-33 pubmed
  81. Govorunova E, Sineshchekov O. Chemotaxis in the green flagellate alga Chlamydomonas. Biochemistry (Mosc). 2005;70:717-25 pubmed
    Behavior of the green flagellate alga Chlamydomonas changes in response to a number of chemical stimuli. Specific sensitivity of the cells to different substances might appear only at certain stages of the life cycle...
  82. Yagi T, Minoura I, Fujiwara A, Saito R, Yasunaga T, Hirono M, et al. An axonemal dynein particularly important for flagellar movement at high viscosity. Implications from a new Chlamydomonas mutant deficient in the dynein heavy chain gene DHC9. J Biol Chem. 2005;280:41412-20 pubmed
    ..In this study, a Chlamydomonas mutant, ida9, lacking inner arm dynein c was isolated and shown to carry a mutation in the DHC9 dynein heavy ..
  83. Wargo M, McPeek M, Smith E. Analysis of microtubule sliding patterns in Chlamydomonas flagellar axonemes reveals dynein activity on specific doublet microtubules. J Cell Sci. 2004;117:2533-44 pubmed
    ..These studies were also conducted using high Ca2+ conditions. In Chlamydomonas, high Ca2+ induces changes in waveform which are predicted to result from regulating dynein activity on ..
  84. Ikeda K, Ikeda T, Morikawa K, Kamiya R. Axonemal localization of Chlamydomonas PACRG, a homologue of the human Parkin-coregulated gene product. Cell Motil Cytoskeleton. 2007;64:814-21 pubmed
    A homologue of mammalian PACRG was identified in Sarkosyl-extracted Chlamydomonas axonemes as a protein that may interact with Rib72 (a component of the protofilament ribbon within the outer doublet microtubules)...
  85. Uniacke J, Zerges W. Photosystem II assembly and repair are differentially localized in Chlamydomonas. Plant Cell. 2007;19:3640-54 pubmed
    ..proteins are synthesized for the de novo assembly and repair of photosystem II (PSII) in the chloroplast of Chlamydomonas reinhardtii, we used fluorescence in situ hybridization, immunofluorescence staining, and confocal microscopy...
  86. Parker J, Quarmby L. Chlamydomonas fla mutants reveal a link between deflagellation and intraflagellar transport. BMC Cell Biol. 2003;4:11 pubmed
    ..involves active severing of the axoneme at the base of the flagellum; this process is defective in Chlamydomonas fa mutants...
  87. Ning J, Otto T, Pfander C, Schwach F, Brochet M, Bushell E, et al. Comparative genomics in Chlamydomonas and Plasmodium identifies an ancient nuclear envelope protein family essential for sexual reproduction in protists, fungi, plants, and vertebrates. Genes Dev. 2013;27:1198-215 pubmed publisher
    ..the broadly conserved protein HAP2 (GCS1) functioned in gamete membrane fusion in the unicellular green alga Chlamydomonas and the malaria pathogen Plasmodium led us to exploit the rare biological phenomenon of isogamy in ..
  88. Richey E, Qin H. Dissecting the sequential assembly and localization of intraflagellar transport particle complex B in Chlamydomonas. PLoS ONE. 2012;7:e43118 pubmed publisher
    ..Prior studies have shown that in Chlamydomonas reinhardtii, IFT46, IFT52, and IFT88 directly interact with each other and are in a subcomplex of the IFT B ..