neurospora crassa


Summary: A species of ascomycetous fungi of the family Sordariaceae, order SORDARIALES, much used in biochemical, genetic, and physiologic studies.

Top Publications

  1. Baker C, Loros J, Dunlap J. The circadian clock of Neurospora crassa. FEMS Microbiol Rev. 2012;36:95-110 pubmed publisher
    ..nearly all major branches of life, except archaebacteria, and within the eukaryotes, the filamentous fungus Neurospora crassa has served for nearly half a century as a durable model organism for uncovering the basic circadian ..
  2. Turner G. Phenotypic analysis of Neurospora crassa gene deletion strains. Methods Mol Biol. 2011;722:191-8 pubmed publisher
    Phenotypic analysis of Neurospora crassa knockout (KO) mutants was used as a vehicle to introduce students to laboratory research...
  3. Yamashita K, Shiozawa A, Watanabe S, Fukumori F, Kimura M, Fujimura M. ATF-1 transcription factor regulates the expression of ccg-1 and cat-1 genes in response to fludioxonil under OS-2 MAP kinase in Neurospora crassa. Fungal Genet Biol. 2008;45:1562-9 pubmed publisher
    ..the atf-1 gene, which encodes an ATF/CREB family transcriptional factor, were isolated and characterized in Neurospora crassa. The characteristic phenotypes in the os-2 MAP kinase strain, such as osmotic sensitivity and fludioxonil ..
  4. Palma Guerrero J, Huang I, Jansson H, Salinas J, Lopez Llorca L, Read N. Chitosan permeabilizes the plasma membrane and kills cells of Neurospora crassa in an energy dependent manner. Fungal Genet Biol. 2009;46:585-94 pubmed publisher
    ..we characterized plasma membrane permeabilization, and cell death and lysis in the experimental model, Neurospora crassa. Rhodamine-labeled chitosan was used to show that chitosan is internalized by fungal cells...
  5. Iyer S, Ramakrishnan M, Kasbekar D. Neurospora crassa fmf-1 encodes the homologue of the Schizosaccharomyces pombe Ste11p regulator of sexual development. J Genet. 2009;88:33-9 pubmed
    The Neurospora crassa fmf-1 mutation exerts an unusual 'perithecium-dominant' developmental arrest; fmf-1 x fmf-1+ cross becomes arrested in perithecial development regardless of whether the mutant participates in the cross as the male ..
  6. Wu W, Hildebrand A, Kasuga T, Xiong X, Fan Z. Direct cellobiose production from cellulose using sextuple beta-glucosidase gene deletion Neurospora crassa mutants. Enzyme Microb Technol. 2013;52:184-9 pubmed publisher
    Direct cellobiose production from cellulose by a genetically modified fungus-Neurospora crassa, was explored in this study. A library of N. crassa sextuple beta-glucosidase (bgl) gene deletion strains was constructed...
  7. Araujo Palomares C, Richthammer C, Seiler S, Castro Longoria E. Functional characterization and cellular dynamics of the CDC-42 - RAC - CDC-24 module in Neurospora crassa. PLoS ONE. 2011;6:e27148 pubmed publisher
    ..In this study, we investigate the role of the CDC-42 - RAC - CDC-24 module in Neurospora crassa. rac and cdc-42 deletion mutants are viable, but generate highly compact colonies with severe morphological ..
  8. Maddi A, Fu C, Free S. The Neurospora crassa dfg5 and dcw1 genes encode ?-1,6-mannanases that function in the incorporation of glycoproteins into the cell wall. PLoS ONE. 2012;7:e38872 pubmed publisher
    ..We demonstrate that the Neurospora crassa DFG5 (NCU03770) and DCW1 (NCU08127) enzymes function in vivo to cross-link glycoproteins into the cell wall...
  9. Bello M, Barrera Perez V, Morin D, Epstein L. The Neurospora crassa mutant Nc?Egt-1 identifies an ergothioneine biosynthetic gene and demonstrates that ergothioneine enhances conidial survival and protects against peroxide toxicity during conidial germination. Fungal Genet Biol. 2012;49:160-72 pubmed publisher
    ..conclusive identification of EGT by LC/MS and the quantification of EGT in Colletotrichum graminicola and Neurospora crassa conidia and mycelia. EGT concentrations were significantly (?=0...

More Information

Publications160 found, 100 shown here

  1. Francis K, Gadda G. Inflated kinetic isotope effects in the branched mechanism of Neurospora crassa 2-nitropropane dioxygenase. Biochemistry. 2009;48:2403-10 pubmed publisher
    Catalytic turnover of Neurospora crassa 2-nitropropane dioxygenase with nitroethane as substrate occurs through both nonoxidative and oxidative pathways...
  2. Lichius A, Lord K, Jeffree C, Oborny R, Boonyarungsrit P, Read N. Importance of MAP kinases during protoperithecial morphogenesis in Neurospora crassa. PLoS ONE. 2012;7:e42565 pubmed publisher
    ..The perithecium, the female sexual fruitbody of Neurospora crassa, differentiates from the vegetative mycelium in distinct morphological stages, and represents one of the ..
  3. Chae M, Nargang F. Investigation of regulatory factors required for alternative oxidase production in Neurospora crassa. Physiol Plant. 2009;137:407-18 pubmed publisher
    ..We have previously isolated mutants in Neurospora crassa that are incapable of inducing AOX...
  4. Li S, Lakin Thomas P. Effects of prd circadian clock mutations on FRQ-less rhythms in Neurospora. J Biol Rhythms. 2010;25:71-80 pubmed publisher
    Rhythmic conidiation (spore formation) in Neurospora crassa provides a model system for investigating the molecular mechanisms of circadian rhythmicity...
  5. Kim J, Kaiser K, Larive C, Borkovich K. Use of 1H nuclear magnetic resonance to measure intracellular metabolite levels during growth and asexual sporulation in Neurospora crassa. Eukaryot Cell. 2011;10:820-31 pubmed publisher
    ..Previous work has demonstrated that all three of the G? subunits in the filamentous fungus Neurospora crassa affect the accumulation of mass on poor carbon sources and that loss of gna-3 leads to the most dramatic ..
  6. Park G, Colot H, Collopy P, Krystofova S, Crew C, Ringelberg C, et al. High-throughput production of gene replacement mutants in Neurospora crassa. Methods Mol Biol. 2011;722:179-89 pubmed publisher
    The model filamentous fungus Neurospora crassa has been the focus of functional genomics studies for the past several years...
  7. Castro Longoria E, Moreno Velázquez S, Vilchis Nestor A, Arenas Berumen E, Avalos Borja M. Production of platinum nanoparticles and nanoaggregates using Neurospora crassa. J Microbiol Biotechnol. 2012;22:1000-4 pubmed
    Fungal biomass and fungal extract of the nonpathogenic fungus Neurospora crassa were successfully used as reducing agents for the biosynthesis of platinum nanoparticles (PtNPs)...
  8. Mouriño Pérez R, Riquelme M. Recent advances in septum biogenesis in Neurospora crassa. Adv Genet. 2013;83:99-134 pubmed publisher
    ..This review summarizes the recent advances in septum ontogenesis in Neurospora crassa. A "septal actomyosin tangle" is the first indication of impending septation...
  9. Dogaris I, Mamma D, Kekos D. Biotechnological production of ethanol from renewable resources by Neurospora crassa: an alternative to conventional yeast fermentations?. Appl Microbiol Biotechnol. 2013;97:1457-73 pubmed publisher
    ..The fungus Neurospora crassa has been used extensively for genetic, biochemical, and molecular studies as a model organism...
  10. Wang Z, Lopez Giraldez F, Lehr N, Farré M, Common R, Trail F, et al. Global gene expression and focused knockout analysis reveals genes associated with fungal fruiting body development in Neurospora crassa. Eukaryot Cell. 2014;13:154-69 pubmed publisher
    ..a model, we used random and multitargeted primers to assay gene expression across perithecial development in Neurospora crassa. We found that functionally unclassified proteins accounted for most upregulated genes, whereas ..
  11. Espagne E, Lespinet O, Malagnac F, Da Silva C, Jaillon O, Porcel B, et al. The genome sequence of the model ascomycete fungus Podospora anserina. Genome Biol. 2008;9:R77 pubmed publisher
    ..The dung-inhabiting ascomycete fungus Podospora anserina is a model used to study various aspects of eukaryotic and fungal biology, such as ageing, prions and sexual development...
  12. Maerz S, Ziv C, Vogt N, Helmstaedt K, Cohen N, Gorovits R, et al. The nuclear Dbf2-related kinase COT1 and the mitogen-activated protein kinases MAK1 and MAK2 genetically interact to regulate filamentous growth, hyphal fusion and sexual development in Neurospora crassa. Genetics. 2008;179:1313-25 pubmed publisher
    Ndr kinases, such as Neurospora crassa COT1, are important for cell differentiation and polar morphogenesis, yet their input signals as well as their integration into a cellular signaling context are still elusive...
  13. Lamb T, Finch K, Bell Pedersen D. The Neurospora crassa OS MAPK pathway-activated transcription factor ASL-1 contributes to circadian rhythms in pathway responsive clock-controlled genes. Fungal Genet Biol. 2012;49:180-8 pubmed publisher
    The OS-pathway mitogen-activated protein kinase (MAPK) cascade of Neurospora crassa is responsible for adaptation to osmotic stress...
  14. Klein A, Israel L, Lackey S, Nargang F, Imhof A, Baumeister W, et al. Characterization of the insertase for ?-barrel proteins of the outer mitochondrial membrane. J Cell Biol. 2012;199:599-611 pubmed publisher
    ..The complex from Neurospora crassa was composed of Tob55-Sam50, Tob38-Sam35, and Tob37-Sam37 in a stoichiometry of 1:1:1 and had a molecular ..
  15. Read N, Lichius A, Shoji J, Goryachev A. Self-signalling and self-fusion in filamentous fungi. Curr Opin Microbiol. 2009;12:608-15 pubmed publisher
    ..This hyphal type in Neurospora crassa is being used as a model for studies on hyphal self-signalling and self-fusion in filamentous fungi...
  16. Maerz S, Funakoshi Y, Negishi Y, Suzuki T, Seiler S. The Neurospora peptide:N-glycanase ortholog PNG1 is essential for cell polarity despite its lack of enzymatic activity. J Biol Chem. 2010;285:2326-32 pubmed publisher
    ..Here we show that the PNG1 ortholog from the filamentous ascomycete Neurospora crassa is an essential protein, and its deletion results in strong polarity defects...
  17. Goyal A, Saxena P, Rahman A, Singh P, Kasbekar D, Gokhale R, et al. Structural insights into biosynthesis of resorcinolic lipids by a type III polyketide synthase in Neurospora crassa. J Struct Biol. 2008;162:411-21 pubmed publisher
    ..Here, we report the structural and mutational investigations of a novel type III PKS protein from Neurospora crassa involved in the biosynthesis of resorcinolic metabolites by utilizing long chain fatty acyl-CoAs...
  18. Honda S, Selker E. Direct interaction between DNA methyltransferase DIM-2 and HP1 is required for DNA methylation in Neurospora crassa. Mol Cell Biol. 2008;28:6044-55 pubmed publisher
    ..Genetics studies of Neurospora crassa have revealed that a DNA methyltransferase (DIM-2), a histone H3K9 methyltransferase (DIM-5), and ..
  19. Rubin Pitel S, Zhang H, Vu T, Brunzelle J, Zhao H, Nair S. Distinct structural elements dictate the specificity of the type III pentaketide synthase from Neurospora crassa. Chem Biol. 2008;15:1079-90 pubmed publisher
    ..These studies establish an unexpected plasticity to the PKS fold, unanticipated from structural studies of other members of this enzyme family. ..
  20. Munoz A, Marcos J, Read N. Concentration-dependent mechanisms of cell penetration and killing by the de novo designed antifungal hexapeptide PAF26. Mol Microbiol. 2012;85:89-106 pubmed publisher
    ..of internalization of the de novo designed cationic hexapeptide PAF26 has been characterized in detail using Neurospora crassa. Live-cell imaging of fluorescently labelled PAF26, organelle probes and mutants indicate that the peptide ..
  21. Pomraning K, Smith K, Freitag M. Genome-wide high throughput analysis of DNA methylation in eukaryotes. Methods. 2009;47:142-50 pubmed publisher high throughput Illumina/Solexa sequencing, exemplified by the analysis of the methylated fraction of the Neurospora crassa genome ("methylome")...
  22. Bae B, Sullivan R, Zhao H, Nair S. Structure and engineering of L-arabinitol 4-dehydrogenase from Neurospora crassa. J Mol Biol. 2010;402:230-40 pubmed publisher
    ..Here we report the crystal structure of LAD from the filamentous fungus Neurospora crassa at 2.6 A resolution. In addition, we created a number of site-directed variants of N...
  23. Rémi J, Merrow M, Roenneberg T. A circadian surface of entrainment: varying T, ?, and photoperiod in Neurospora crassa. J Biol Rhythms. 2010;25:318-28 pubmed publisher
    ..The authors therefore investigated phase of entrainment for 162 combinations of T, ?, and photoperiod in Neurospora crassa, which lends itself to a high-throughput approach...
  24. Carneiro P, Duarte M, Videira A. Disruption of alternative NAD(P)H dehydrogenases leads to decreased mitochondrial ROS in Neurospora crassa. Free Radic Biol Med. 2012;52:402-9 pubmed publisher
    ..The involvement of Neurospora crassa alternative NAD(P)H dehydrogenases in mitochondrial ROS production was evaluated...
  25. Hamam A, Lew R. Electrical phenotypes of calcium transport mutant strains of a filamentous fungus, Neurospora crassa. Eukaryot Cell. 2012;11:694-702 pubmed publisher
    ..Thus, although Ca(2+) homeostasis is perturbed in the nca-2 mutant (B. J. Bowman et al., Eukaryot. Cell 10:654-661, 2011), the phenotype does not extend to tip growth or to osmoregulation but is revealed by lower H(+)-ATPase activity. ..
  26. Hutchison E, Brown S, Tian C, Glass N. Transcriptional profiling and functional analysis of heterokaryon incompatibility in Neurospora crassa reveals that reactive oxygen species, but not metacaspases, are associated with programmed cell death. Microbiology. 2009;155:3957-70 pubmed publisher
    ..To understand processes associated with HI and PCD, we used whole-genome microarrays for Neurospora crassa to assess transcriptional differences associated with induction of HI mediated by differences in het-c pin-c ..
  27. Lee B, Yoshida Y, Hasunuma K. Nucleoside diphosphate kinase-1 regulates hyphal development via the transcriptional regulation of catalase in Neurospora crassa. FEBS Lett. 2009;583:3291-5 pubmed publisher
    ..Furthermore the ndk-1(RIP-1) mutants could form thick hyphae by oxidative stress and a disruption of cat-3. These results suggest that the loss of thick hyphae in the ndk-1(RIP-1) mutants may be caused by the over-expression of cat-3...
  28. Freitas F, de Paula R, Barbosa L, Terenzi H, Bertolini M. cAMP signaling pathway controls glycogen metabolism in Neurospora crassa by regulating the glycogen synthase gene expression and phosphorylation. Fungal Genet Biol. 2010;47:43-52 pubmed publisher
    ..In this work we investigated its role in the Neurospora crassa glycogen metabolism control using mutant strains affected in components of the pathway, the cr-1 strain ..
  29. Greenwald C, Kasuga T, Glass N, Shaw B, Ebbole D, Wilkinson H. Temporal and spatial regulation of gene expression during asexual development of Neurospora crassa. Genetics. 2010;186:1217-30 pubmed publisher
    ..profiled spatial and temporal transcriptional changes during asexual sporulation in the filamentous fungus Neurospora crassa. Aerial tissue was separated from the mycelium to allow detection of genes specific to each tissue...
  30. Zhang Y, Zhang Z, Zhang X, Zhang H, Sun X, Hu C, et al. CDR4 is the major contributor to azole resistance among four Pdr5p-like ABC transporters in Neurospora crassa. Fungal Biol. 2012;116:848-54 pubmed publisher
    ..b>Neurospora crassa has a total of four genes encoding Pdr5p homologues including CDR4 (PMR1-like), ATRB (AtrB-like), and ATRF (..
  31. Riquelme M, Yarden O, Bartnicki Garcia S, BOWMAN B, Castro Longoria E, Free S, et al. Architecture and development of the Neurospora crassa hypha -- a model cell for polarized growth. Fungal Biol. 2011;115:446-74 pubmed publisher
    b>Neurospora crassa has been at the forefront of biological research from the early days of biochemical genetics to current progress being made in understanding gene and genetic network function...
  32. Lambreghts R, Shi M, Belden W, DeCaprio D, Park D, Henn M, et al. A high-density single nucleotide polymorphism map for Neurospora crassa. Genetics. 2009;181:767-81 pubmed publisher the discovery and validation of a set of single nucleotide polymorphisms (SNPs) between the reference Neurospora crassa strain Oak Ridge and the Mauriceville strain (FGSC 2555), of sufficient density to allow fine mapping of ..
  33. Wright S, Inchausti R, Eaton C, Krystofova S, Borkovich K. RIC8 is a guanine-nucleotide exchange factor for Galpha subunits that regulates growth and development in Neurospora crassa. Genetics. 2011;189:165-76 pubmed publisher
    ..RIC8 is present in animals and filamentous fungi, such as the model eukaryote Neurospora crassa, but is absent from the genomes of baker's yeast and plants...
  34. Maerz S, Dettmann A, Seiler S. Hydrophobic motif phosphorylation coordinates activity and polar localization of the Neurospora crassa nuclear Dbf2-related kinase COT1. Mol Cell Biol. 2012;32:2083-98 pubmed publisher
    ..We demonstrate that the Neurospora crassa NDR kinase COT1 forms inactive dimers via a conserved N-terminal extension, which is also required for the ..
  35. Leeder A, Jonkers W, Li J, Glass N. Early colony establishment in Neurospora crassa requires a MAP kinase regulatory network. Genetics. 2013;195:883-98 pubmed publisher
    ..In the ascomycete fungus Neurospora crassa, vegetative fusion occurs between germinated conidia (germlings) via specialized structures termed "..
  36. Xu H, Wang J, Hu Q, Quan Y, Chen H, Cao Y, et al. DCAF26, an adaptor protein of Cul4-based E3, is essential for DNA methylation in Neurospora crassa. PLoS Genet. 2010;6:e1001132 pubmed publisher
    ..Genetic studies in Neurospora crassa previously showed that the CUL4-DDB1 E3 ubiquitin ligase regulates DNA methylation via histone H3K9 ..
  37. Takata R, Tokita K, Mori S, Shimoda R, Harada N, Ichinose H, et al. Degradation of carbohydrate moieties of arabinogalactan-proteins by glycoside hydrolases from Neurospora crassa. Carbohydr Res. 2010;345:2516-22 pubmed publisher
    ..hydrolase family (GHF) 54, NcAraf1, and an endo-?-(1?6)-galactanase of GHF 5, Nc6GAL, were identified in Neurospora crassa. Recombinant NcAraf1 (rNcAraf1) expressed in Pichia pastoris hydrolyzed radish AGPs as well as arabinan and ..
  38. Takeda A, Freitas F, Magro A, Bernardes N, Fernandes C, Gon alves R, et al. Biophysical characterization of the recombinant importin-? from Neurospora crassa. Protein Pept Lett. 2013;20:8-16 pubmed
    b>Neurospora crassa has been widely used as a model organism and contributed to the development of biochemistry and molecular biology by allowing the identification of many metabolic pathways and mechanisms responsible for gene regulation...
  39. Eaton C, Cabrera I, Servin J, Wright S, Cox M, Borkovich K. The guanine nucleotide exchange factor RIC8 regulates conidial germination through G? proteins in Neurospora crassa. PLoS ONE. 2012;7:e48026 pubmed publisher
    Heterotrimeric G protein signaling is essential for normal hyphal growth in the filamentous fungus Neurospora crassa. We have previously demonstrated that the non-receptor guanine nucleotide exchange factor RIC8 acts upstream of the G? ..
  40. Gessler N, Rudchenko M, Belozerskaia T. [Stress factor-induced changes in the activity of antioxidant protective mechanisms in the wild type strain of Neurospora crassa and in its photoreceptor complex mutants]. Mikrobiologiia. 2008;77:163-70 pubmed well as on the content of thiol and disulfide groups in low-molecular-weight compounds and proteins of Neurospora crassa mycelium was studied in the wild type strain and white collar-1 (wc-1) and white collar-2 (wc-2) mutants...
  41. Xiong L, Adhvaryu K, Selker E, Wang Y. Mapping of lysine methylation and acetylation in core histones of Neurospora crassa. Biochemistry. 2010;49:5236-43 pubmed publisher
    ..In contrast, the histone modifications in Neurospora crassa, a convenient model organism for multicellular eukaryotes, remained largely undefined...
  42. Froehlich A, Chen C, Belden W, Madeti C, Roenneberg T, Merrow M, et al. Genetic and molecular characterization of a cryptochrome from the filamentous fungus Neurospora crassa. Eukaryot Cell. 2010;9:738-50 pubmed publisher
    ..We have examined the cryptochrome from the filamentous fungus Neurospora crassa and demonstrate that Neurospora cry encodes a DASH-type cryptochrome that appears capable of binding flavin ..
  43. Baker C, Kettenbach A, Loros J, Gerber S, Dunlap J. Quantitative proteomics reveals a dynamic interactome and phase-specific phosphorylation in the Neurospora circadian clock. Mol Cell. 2009;34:354-63 pubmed publisher
  44. Rountree M, Selker E. DNA methylation and the formation of heterochromatin in Neurospora crassa. Heredity (Edinb). 2010;105:38-44 pubmed publisher
    Studies of the control and function of DNA methylation in Neurospora crassa have led to a greater understanding of heterochromatin formation...
  45. Roca M, Kuo H, Lichius A, Freitag M, Read N. Nuclear dynamics, mitosis, and the cytoskeleton during the early stages of colony initiation in Neurospora crassa. Eukaryot Cell. 2010;9:1171-83 pubmed publisher
    b>Neurospora crassa macroconidia form germ tubes that are involved in colony establishment and conidial anastomosis tubes (CATs) that fuse to form interconnected networks of conidial germlings...
  46. Lewis Z, Adhvaryu K, Honda S, Shiver A, Selker E. Identification of DIM-7, a protein required to target the DIM-5 H3 methyltransferase to chromatin. Proc Natl Acad Sci U S A. 2010;107:8310-5 pubmed publisher
    ..We previously demonstrated that heterochromatin in the filamentous fungus Neurospora crassa is marked by cytosine methylation directed by trimethylated Lysine 9 on histone H3 (H3K9me3)...
  47. Troppens D, Chu M, Holcombe L, Gleeson O, O Gara F, Read N, et al. The bacterial secondary metabolite 2,4-diacetylphloroglucinol impairs mitochondrial function and affects calcium homeostasis in Neurospora crassa. Fungal Genet Biol. 2013;56:135-46 pubmed publisher
    ..The mechanism by which this occurs is unknown and in this study the filamentous fungus Neurospora crassa was used as a model to investigate the effects of DAPG on a eukaryotic cell...
  48. Wang J, Hu Q, Chen H, Zhou Z, Li W, Wang Y, et al. Role of individual subunits of the Neurospora crassa CSN complex in regulation of deneddylation and stability of cullin proteins. PLoS Genet. 2010;6:e1001232 pubmed publisher
    ..We found seven CSN subunits in Neurospora crassa in a previous study, but only one subunit, CSN-2, was functionally characterized...
  49. Bowman B, Abreu S, Margolles Clark E, Draskovic M, Bowman E. Role of four calcium transport proteins, encoded by nca-1, nca-2, nca-3, and cax, in maintaining intracellular calcium levels in Neurospora crassa. Eukaryot Cell. 2011;10:654-61 pubmed publisher
    We have examined the distribution of calcium in Neurospora crassa and investigated the role of four predicted calcium transport proteins...
  50. Arnett D, Lorimer H, Asch D. Catabolite repression directly affects transcription of the qa-y gene of Neurospora crassa. Fungal Genet Biol. 2009;46:377-80 pubmed publisher
    The quinic acid (qa) gene cluster of Neurospora crassa is subject to two levels of gene control: a primary system which responds to the presence of quinic acid via the qa-1S repressor protein, and a secondary system which represses ..
  51. Wu C, Kim Y, Smith K, Li W, Hood H, Staben C, et al. Characterization of chromosome ends in the filamentous fungus Neurospora crassa. Genetics. 2009;181:1129-45 pubmed publisher
    ..Prior to initiation of this project, the Neurospora crassa genome assembly contained only 3 of the 14 telomeres...
  52. Diernfellner A, Querfurth C, Salazar C, Hofer T, Brunner M. Phosphorylation modulates rapid nucleocytoplasmic shuttling and cytoplasmic accumulation of Neurospora clock protein FRQ on a circadian time scale. Genes Dev. 2009;23:2192-200 pubmed publisher
    ..We present a mathematical model that describes the cytoplasmic accumulation of wild-type and mutant FRQ on a circadian time scale on the basis of frequency-modulated rapid nucleocytoplasmic shuttling cycles. ..
  53. Du J, Li S, Zhao H. Discovery and characterization of novel d-xylose-specific transporters from Neurospora crassa and Pichia stipitis. Mol Biosyst. 2010;6:2150-6 pubmed publisher
    ..Here we report the cloning and characterization of two novel d-xylose-specific transporters from Neurospora crassa and Pichia stipitis. These two transporters were identified from a total of 18 putative pentose transporters...
  54. Vaidya A, Chen C, Dunlap J, Loros J, Crane B. Structure of a light-activated LOV protein dimer that regulates transcription. Sci Signal. 2011;4:ra50 pubmed publisher
    ..The LOV protein VIVID (VVD) of the filamentous fungus Neurospora crassa enables the organism to adapt to constant or increasing amounts of light and facilitates proper entrainment ..
  55. Sun J, Tian C, Diamond S, Glass N. Deciphering transcriptional regulatory mechanisms associated with hemicellulose degradation in Neurospora crassa. Eukaryot Cell. 2012;11:482-93 pubmed publisher
    ..b>Neurospora crassa, a model filamentous fungus, expresses and secretes enzymes required for plant cell wall deconstruction...
  56. Shi M, Collett M, Loros J, Dunlap J. FRQ-interacting RNA helicase mediates negative and positive feedback in the Neurospora circadian clock. Genetics. 2010;184:351-61 pubmed publisher
    ..Moreover, the results suggest that there are clock-specific roles for FRH that are distinct from the predicted essential exosome-associated functions for the protein. ..
  57. Ellison C, Hall C, Kowbel D, Welch J, Brem R, Glass N, et al. Population genomics and local adaptation in wild isolates of a model microbial eukaryote. Proc Natl Acad Sci U S A. 2011;108:2831-6 pubmed publisher
    ..Here, we have circumvented these issues in the microbial eukaryote Neurospora crassa by using a "reverse-ecology" population genomic approach that is free of a priori assumptions ..
  58. Lackey S, Wideman J, Kennedy E, Go N, Nargang F. The Neurospora crassa TOB complex: analysis of the topology and function of Tob38 and Tob37. PLoS ONE. 2011;6:e25650 pubmed publisher
    ..We have identified several forms of the complex in Neurospora crassa. One form contains Tob55, Tob38, and Tob37; another contains these three subunits plus the Mdm10 protein; ..
  59. Phillips C, Beeson W, CATE J, Marletta M. Cellobiose dehydrogenase and a copper-dependent polysaccharide monooxygenase potentiate cellulose degradation by Neurospora crassa. ACS Chem Biol. 2011;6:1399-406 pubmed publisher
    ..Deletion of cdh-1, the gene encoding the major cellobiose dehydrogenase of Neurospora crassa, reduced cellulase activity substantially, and addition of purified cellobiose dehydrogenases from M...
  60. Squina F, Leal J, Cipriano V, Martinez Rossi N, Rossi A. Transcription of the Neurospora crassa 70-kDa class heat shock protein genes is modulated in response to extracellular pH changes. Cell Stress Chaperones. 2010;15:225-31 pubmed publisher
    ..Moreover, this gene was not induced by heat shock. These results reveal novel aspects of the heat-sensing network of N. crassa. ..
  61. Verdin J, Bartnicki Garcia S, Riquelme M. Functional stratification of the Spitzenkörper of Neurospora crassa. Mol Microbiol. 2009;74:1044-53 pubmed publisher
    GS-1 (ncu04189) is a protein required for the synthesis of beta-1,3-glucan in Neurospora crassa. As chitin, beta-1,3-glucan is a morphogenetically relevant component of the fungal cell wall...
  62. Chen C, Dunlap J, Loros J. Neurospora illuminates fungal photoreception. Fungal Genet Biol. 2010;47:922-9 pubmed publisher
    ..This review will briefly introduce the basic light sensing machinery in Neurospora crassa, a filamentous fungus, and then focus on the most recent advances in employing Neurospora as a model to ..
  63. Paietta J. DNA-binding specificity of the CYS3 transcription factor of Neurospora crassa defined by binding-site selection. Fungal Genet Biol. 2008;45:1166-71 pubmed publisher
    ..of a coordinately regulated group of genes involved in the acquisition and utilization of sulfur in Neurospora crassa. An approach of using binding-site selection from random-sequence oligonucleotides was used to define CYS3-..
  64. Cha J, Chang S, Huang G, Cheng P, Liu Y. Control of WHITE COLLAR localization by phosphorylation is a critical step in the circadian negative feedback process. EMBO J. 2008;27:3246-55 pubmed publisher
  65. Schneider K, Perrino S, Oelhafen K, Li S, Zatsepin A, Lakin Thomas P, et al. Rhythmic conidiation in constant light in vivid mutants of Neurospora crassa. Genetics. 2009;181:917-31 pubmed publisher
    In Neurospora crassa, a circadian rhythm of conidiation (asexual spore formation) can be seen on the surface of agar media. This rhythm has a period of 22 hr in constant darkness (D/D)...
  66. Nowrousian M, Stajich J, Chu M, Engh I, Espagne E, Halliday K, et al. De novo assembly of a 40 Mb eukaryotic genome from short sequence reads: Sordaria macrospora, a model organism for fungal morphogenesis. PLoS Genet. 2010;6:e1000891 pubmed publisher
    ..The S. macrospora genome contains even fewer repeat regions than its closest sequenced relative, Neurospora crassa. Comparison with genomes of other fungi showed that S...
  67. Lee H, Aalto A, Yang Q, Chang S, Huang G, Fisher D, et al. The DNA/RNA-dependent RNA polymerase QDE-1 generates aberrant RNA and dsRNA for RNAi in a process requiring replication protein A and a DNA helicase. PLoS Biol. 2010;8: pubmed publisher
    ..Together, these results suggest a novel small RNA biogenesis pathway in Neurospora and a new mechanism for the production of aRNA and dsRNA in RNAi pathways. ..
  68. Lamb T, Goldsmith C, Bennett L, Finch K, Bell Pedersen D. Direct transcriptional control of a p38 MAPK pathway by the circadian clock in Neurospora crassa. PLoS ONE. 2011;6:e27149 pubmed publisher
    ..Thus, circadian transcriptional control of MAPK pathway components leads to striking time-of-day-specific effects on the signaling status and physiological response of the pathway. ..
  69. Hutchison E, Glass N. Meiotic regulators Ndt80 and ime2 have different roles in Saccharomyces and Neurospora. Genetics. 2010;185:1271-82 pubmed publisher
    Meiosis is a highly regulated process in eukaryotic species. The filamentous fungus Neurospora crassa has been shown to be missing homologs of a number of meiotic initiation genes conserved in Saccharomyces cerevisiae, but has three ..
  70. Li S, Motavaze K, Kafes E, Suntharalingam S, Lakin Thomas P. A new mutation affecting FRQ-less rhythms in the circadian system of Neurospora crassa. PLoS Genet. 2011;7:e1002151 pubmed publisher
    We are using the fungus Neurospora crassa as a model organism to study the circadian system of eukaryotes...
  71. Li X, Beeson W, Phillips C, Marletta M, Cate J. Structural basis for substrate targeting and catalysis by fungal polysaccharide monooxygenases. Structure. 2012;20:1051-61 pubmed publisher
    ..Here, we present structures of Neurospora crassa PMO-2 and PMO-3 at 1.10 and 1.37 Å resolution, respectively...
  72. Wideman J, Go N, Klein A, Redmond E, Lackey S, Tao T, et al. Roles of the Mdm10, Tom7, Mdm12, and Mmm1 proteins in the assembly of mitochondrial outer membrane proteins in Neurospora crassa. Mol Biol Cell. 2010;21:1725-36 pubmed publisher
    ..Here we show that loss of any of these three proteins in Neurospora crassa results in the formation of large mitochondrial tubules and reduces the assembly of porin and Tom40 into the ..
  73. Chung D, Greenwald C, Upadhyay S, Ding S, Wilkinson H, Ebbole D, et al. acon-3, the Neurospora crassa ortholog of the developmental modifier, medA, complements the conidiation defect of the Aspergillus nidulans mutant. Fungal Genet Biol. 2011;48:370-6 pubmed publisher
    Aspergillus nidulans and Neurospora crassa are ascomycetes that produce asexual spores through morphologically distinct processes. MedA, a protein with unknown function, is required for normal asexual and sexual development in A...
  74. Brenna A, Grimaldi B, Filetici P, Ballario P. Physical association of the WC-1 photoreceptor and the histone acetyltransferase NGF-1 is required for blue light signal transduction in Neurospora crassa. Mol Biol Cell. 2012;23:3863-72 pubmed publisher
    In Neurospora crassa and other filamentous fungi, light-dependent-specific phenomena are regulated by transcription factors WC-1 and WC-2...
  75. Pinto D, Duarte M, Soares S, Tropschug M, Videira A. Identification of all FK506-binding proteins from Neurospora crassa. Fungal Genet Biol. 2008;45:1600-7 pubmed publisher
    ..Four immunophilins of the FKBP-type (FK506-binding proteins) were identified in the genome of Neurospora crassa. Previously, FKBP22 has been located in the endoplasmic reticulum as part of chaperone/folding complexes and ..
  76. Hong C, Ruoff P, Loros J, Dunlap J. Closing the circadian negative feedback loop: FRQ-dependent clearance of WC-1 from the nucleus. Genes Dev. 2008;22:3196-204 pubmed publisher
    In Neurospora crassa, a transcription factor, WCC, activates the transcription of frq...
  77. Adhvaryu K, Selker E. Protein phosphatase PP1 is required for normal DNA methylation in Neurospora. Genes Dev. 2008;22:3391-6 pubmed publisher
    ..To examine the relationship between H3S10p, H3K9me, and DNA methylation in Neurospora crassa, we built and tested mutants of the putative H3S10 phosphatase, PP1...
  78. Yang Q, Li L, Xue Z, Ye Q, Zhang L, Li S, et al. Transcription of the major neurospora crassa microRNA-like small RNAs relies on RNA polymerase III. PLoS Genet. 2013;9:e1003227 pubmed publisher
    ..We previously discovered miRNA-like small RNAs (milRNAs) in the filamentous fungus Neurospora crassa and uncovered at least four different pathways for milRNA production...
  79. Van Diepeningen A, Pal K, Van der Lee T, Hoekstra R, Debets A. The het-c heterokaryon incompatibility gene in Aspergillus niger. Mycol Res. 2009;113:222-9 pubmed publisher
    ..niger. To test whether the genes that determine heterokaryon incompatibility in Neurospora crassa, such as het-c, vib-1 and pin-c, have a similar function in A...
  80. Sancar G, Sancar C, Brunner M, Schafmeier T. Activity of the circadian transcription factor White Collar Complex is modulated by phosphorylation of SP-motifs. FEBS Lett. 2009;583:1833-40 pubmed publisher
    ..The White Collar Complex (WCC) is the central transcription factor of the circadian clock of Neurospora crassa. Its activity is regulated in a circadian manner by rhythmic phosphorylation, mediated by the clock protein ..
  81. Lee H, Chang S, Choudhary S, Aalto A, Maiti M, Bamford D, et al. qiRNA is a new type of small interfering RNA induced by DNA damage. Nature. 2009;459:274-7 pubmed publisher
    ..the expression of the Argonaute protein QDE-2 and a new class of small RNAs in the filamentous fungus Neurospora crassa. This class of small RNAs, known as qiRNAs because of their interaction with QDE-2, are about 20-21 ..
  82. Collopy P, Colot H, Park G, Ringelberg C, Crew C, Borkovich K, et al. High-throughput construction of gene deletion cassettes for generation of Neurospora crassa knockout strains. Methods Mol Biol. 2010;638:33-40 pubmed publisher
    ..Knockout cassettes for all annotated ORFs in Neurospora crassa were constructed using yeast recombinational cloning...
  83. Honda S, Selker E. Tools for fungal proteomics: multifunctional neurospora vectors for gene replacement, protein expression and protein purification. Genetics. 2009;182:11-23 pubmed publisher
    ..expression vectors for detection and isolation of proteins and protein complexes in the filamentous fungus Neurospora crassa. The vectors, which can be adapted for other fungi, contain C- or N-terminal FLAG, HA, Myc, GFP, or HAT-FLAG ..
  84. Galazka J, Tian C, Beeson W, Martinez B, Glass N, Cate J. Cellodextrin transport in yeast for improved biofuel production. Science. 2010;330:84-6 pubmed publisher
    ..We show that the model cellulolytic fungus Neurospora crassa relies on a high-affinity cellodextrin transport system for rapid growth on cellulose...
  85. Khatun R, Lakin Thomas P. Activation and localization of protein kinase C in Neurospora crassa. Fungal Genet Biol. 2011;48:465-73 pubmed publisher
    ..However, over-expression of NPKC did not lengthen the conidiation period, indicating that PKC in Neurospora may not be responsible for the lengthened period in low choline cultures. ..
  86. Cha J, Yuan H, Liu Y. Regulation of the activity and cellular localization of the circadian clock protein FRQ. J Biol Chem. 2011;286:11469-78 pubmed publisher
    ..Our results indicate that the rhythmic FRQ phosphorylation profile observed is an important part of the negative feedback mechanism that drives robust circadian gene expression...
  87. Hurley J, Chen C, Loros J, Dunlap J. Light-inducible system for tunable protein expression in Neurospora crassa. G3 (Bethesda). 2012;2:1207-12 pubmed publisher
    ..In the model organism Neurospora crassa, several regulatable promoters have been used for heterologous gene expression but all suffer from leaky ..
  88. Castro A, Lemos C, Falcão A, Glass N, Videira A. Increased resistance of complex I mutants to phytosphingosine-induced programmed cell death. J Biol Chem. 2008;283:19314-21 pubmed publisher
    We have studied the effects of phytosphingosine (PHS) on cells of the filamentous fungus Neurospora crassa. Highly reduced viability, impairment of asexual spore germination, DNA condensation and fragmentation, and production of reactive ..
  89. Fleissner A, Diamond S, Glass N. The Saccharomyces cerevisiae PRM1 homolog in Neurospora crassa is involved in vegetative and sexual cell fusion events but also has postfertilization functions. Genetics. 2009;181:497-510 pubmed publisher
    ..In the filamentous fungus Neurospora crassa, cell fusion events occur during both vegetative and sexual stages of its life cycle, thus making it an ..
  90. Lewis Z, Honda S, Khlafallah T, Jeffress J, Freitag M, Mohn F, et al. Relics of repeat-induced point mutation direct heterochromatin formation in Neurospora crassa. Genome Res. 2009;19:427-37 pubmed publisher
    ..b>Neurospora crassa sports features of heterochromatin found in higher eukaryotes, namely cytosine methylation (5mC), ..
  91. Smith K, Dobosy J, Reifsnyder J, Rountree M, Anderson D, Green G, et al. H2B- and H3-specific histone deacetylases are required for DNA methylation in Neurospora crassa. Genetics. 2010;186:1207-16 pubmed publisher
    b>Neurospora crassa utilizes DNA methylation to inhibit transcription of heterochromatin...
  92. Beeson W, Phillips C, Cate J, Marletta M. Oxidative cleavage of cellulose by fungal copper-dependent polysaccharide monooxygenases. J Am Chem Soc. 2012;134:890-2 pubmed publisher
    ..Different PMOs isolated from Neurospora crassa were found to generate oxidized cellodextrins modified at the reducing or nonreducing ends upon incubation ..