yarrowia

Summary

Summary: A genus of ascomycetous yeast in the family Dipodascaceae, order SACCHAROMYCETALES.

Top Publications

  1. Beopoulos A, Cescut J, Haddouche R, Uribelarrea J, Molina Jouve C, Nicaud J. Yarrowia lipolytica as a model for bio-oil production. Prog Lipid Res. 2009;48:375-87 pubmed publisher
  2. Ni X, Yue L, Chi Z, Li J, Wang X, Madzak C. Alkaline protease gene cloning from the marine yeast Aureobasidium pullulans HN2-3 and the protease surface display on Yarrowia lipolytica for bioactive peptide production. Mar Biotechnol (NY). 2009;11:81-9 pubmed publisher
    ..cloned into the multiple cloning sites of the surface display vector pINA1317-YlCWP110 and expressed in cells of Yarrowia lipolytica, the cells displaying protease could form a clear zone on the double plate containing milk protein and ..
  3. Cunha A, Fernandez Lorente G, Bevilaqua J, Destain J, Paiva L, Freire D, et al. Immobilization of Yarrowia lipolytica lipase--a comparison of stability of physical adsorption and covalent attachment techniques. Appl Biochem Biotechnol. 2008;146:49-56 pubmed publisher
    ..This work evaluated the immobilization of a highly active Yarrowia lipolytica lipase (YLL) by physical adsorption and covalent attachment...
  4. Haddouche R, Delessert S, Sabirova J, Neuvéglise C, Poirier Y, Nicaud J. Roles of multiple acyl-CoA oxidases in the routing of carbon flow towards ?-oxidation and polyhydroxyalkanoate biosynthesis in Yarrowia lipolytica. FEMS Yeast Res. 2010;10:917-27 pubmed publisher
    The oleaginous yeast Yarrowia lipolytica possesses six acyl-CoA oxidase (Aox) isoenzymes encoded by genes POX1-POX6. The respective roles of these multiple Aox isoenzymes were studied in recombinant Y...
  5. Najjar A, Robert S, Guérin C, Violet Asther M, Carriere F. Quantitative study of lipase secretion, extracellular lipolysis, and lipid storage in the yeast Yarrowia lipolytica grown in the presence of olive oil: analogies with lipolysis in humans. Appl Microbiol Biotechnol. 2011;89:1947-62 pubmed publisher
    Lipase secretion, extracellular lipolysis, and fatty acid uptake were quantified in the yeast Yarrowia lipolytica grown in the presence of olive oil and/or glucose...
  6. Shinde N, Bankar A, Kumar A, Zinjarde S. Removal of Ni (II) ions from aqueous solutions by biosorption onto two strains of Yarrowia lipolytica. J Environ Manage. 2012;102:115-24 pubmed publisher
    The removal of Ni (II) ions from aqueous solutions by two strains of Yarrowia lipolytica (NCIM 3589 and NCIM 3590) under different environmental conditions was studied...
  7. Mentel M, Piskur J, Neuvéglise C, Rycovska A, Cellengová G, Kolarov J. Triplicate genes for mitochondrial ADP/ATP carriers in the aerobic yeast Yarrowia lipolytica are regulated differentially in the absence of oxygen. Mol Genet Genomics. 2005;273:84-91 pubmed
    b>Yarrowia lipolytica is a strictly aerobic fungus, which differs from the extensively studied model yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe with respect to its physiology, genetics and dimorphic growth habit...
  8. Clason T, Ruiz T, Schagger H, Peng G, Zickermann V, Brandt U, et al. The structure of eukaryotic and prokaryotic complex I. J Struct Biol. 2010;169:81-8 pubmed publisher
    ..microscopy, and have been analyzed in comparison with the previously determined structure of Complex I from Yarrowia lipolytica...
  9. Morín M, Monteoliva L, Insenser M, Gil C, Dominguez A. Proteomic analysis reveals metabolic changes during yeast to hypha transition in Yarrowia lipolytica. J Mass Spectrom. 2007;42:1453-62 pubmed
    ..The ascomycete Yarrowia lipolytica can grow as yeast, pseudomycelial or mycelial forms. We have used a Y...

More Information

Publications151 found, 100 shown here

  1. Liu G, Yue L, Chi Z, Yu W, Chi Z, Madzak C. The surface display of the alginate lyase on the cells of Yarrowia lipolytica for hydrolysis of alginate. Mar Biotechnol (NY). 2009;11:619-26 pubmed publisher
    ..cloned into the multiple cloning site of the surface display vector pINA1317-YlCWP110 and expressed in cells of Yarrowia lipolytica, the cells displaying the alginate lyase could form clear zone on the plate containing sodium alginate,..
  2. Hirakawa K, Kobayashi S, Inoue T, Endoh Yamagami S, Fukuda R, Ohta A. Yas3p, an Opi1 family transcription factor, regulates cytochrome P450 expression in response to n-alkanes in Yarrowia lipolytica. J Biol Chem. 2009;284:7126-37 pubmed publisher
    In the alkane-assimilating yeast Yarrowia lipolytica, the expression of ALK1, a gene encoding cytochrome P450 that catalyzes the first step of n-alkane oxidation, is induced by n-alkanes...
  3. Blanchin Roland S, Da Costa G, Gaillardin C. ESCRT-I components of the endocytic machinery are required for Rim101-dependent ambient pH regulation in the yeast Yarrowia lipolytica. Microbiology. 2005;151:3627-37 pubmed
    ..Insertional mutagenesis in the yeast Yarrowia lipolytica identified two components of the endosome-associated ESCRT-I complex involved in multivesicular body (..
  4. Yan M, Rachubinski D, Joshi S, Rachubinski R, Subramani S. Dysferlin domain-containing proteins, Pex30p and Pex31p, localized to two compartments, control the number and size of oleate-induced peroxisomes in Pichia pastoris. Mol Biol Cell. 2008;19:885-98 pubmed
    b>Yarrowia lipolytica Pex23p and Saccharomyces cerevisiae Pex30p, Pex31p, and Pex32p comprise a family of dysferlin domain-containing peroxins...
  5. Braun A, Geier M, Bühler B, Schmid A, Mauersberger S, Glieder A. Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes. Microb Cell Fact. 2012;11:106 pubmed publisher
    b>Yarrowia lipolytica efficiently metabolizes and assimilates hydrophobic compounds such as n-alkanes and fatty acids...
  6. Juszczyk P, Tomaszewska L, Kita A, Rymowicz W. Biomass production by novel strains of Yarrowia lipolytica using raw glycerol, derived from biodiesel production. Bioresour Technol. 2013;137:124-31 pubmed publisher
    This study demonstrated the potential applicability of the isolated strains of Yarrowia lipolytica for the valorization of glycerol waste generated during biodiesel production, throughout biomass production...
  7. Li X, Wang P, Ge Y, Wang W, Abbas A, Zhu G. NADP(+)-specific isocitrate dehydrogenase from oleaginous yeast Yarrowia lipolytica CLIB122: biochemical characterization and coenzyme sites evaluation. Appl Biochem Biotechnol. 2013;171:403-16 pubmed publisher
    NADP(+)-dependent isocitrate dehydrogenase from Yarrowia lipolytica CLIB122 (YlIDP) was overexpressed and purified. The molecular mass of YlIDP was estimated to be about 81.3 kDa, suggesting its homodimeric structure in solution...
  8. Parfene G, Horincar V, Tyagi A, Malik A, Bahrim G. Production of medium chain saturated fatty acids with enhanced antimicrobial activity from crude coconut fat by solid state cultivation of Yarrowia lipolytica. Food Chem. 2013;136:1345-9 pubmed publisher
    ..of crude coconut fat hydrolysates obtained in solid-state cultivation system with a selected yeast strain Yarrowia lipolytica RO13 were performed...
  9. Madzak C, Otterbein L, Chamkha M, Moukha S, Asther M, Gaillardin C, et al. Heterologous production of a laccase from the basidiomycete Pycnoporus cinnabarinus in the dimorphic yeast Yarrowia lipolytica. FEMS Yeast Res. 2005;5:635-46 pubmed
    Pycnoporus cinnabarinus lac1 gene was expressed in Yarrowia lipolytica. Different secretion signals and culture media were tested...
  10. Ito H, Inouhe M, Tohoyama H, Joho M. Characteristics of copper tolerance in Yarrowia lipolytica. Biometals. 2007;20:773-80 pubmed
    We discovered that a mutant strain of the dimorphic yeast Yarrowia lipolytica could grow in the yeast form in high concentrations of copper sulfate. The amount of metal accumulated by Y...
  11. Papanikolaou S, Galiotou Panayotou M, Fakas S, Komaitis M, Aggelis G. Citric acid production by Yarrowia lipolytica cultivated on olive-mill wastewater-based media. Bioresour Technol. 2008;99:2419-28 pubmed
    b>Yarrowia lipolytica ACA-DC 50109 cultivated on olive-mill wastewater (O.M.W.)-based media, enriched with commercial-industrial glucose, presented an efficient cell growth. Parameters of growth were unaffected by the presence of O.M...
  12. Lopes M, Gomes N, Goncalves C, Coelho M, Mota M, Belo I. Yarrowia lipolytica lipase production enhanced by increased air pressure. Lett Appl Microbiol. 2008;46:255-60 pubmed
    To study the cellular growth and morphology of Yarrowia lipolytica W29 and its lipase and protease production under increased air pressures...
  13. Lazar Z, Walczak E, Robak M. Simultaneous production of citric acid and invertase by Yarrowia lipolytica SUC+ transformants. Bioresour Technol. 2011;102:6982-9 pubmed publisher
    Simultaneous production of citric acid (CA) and invertase by Yarrowia lipolytica A-101-B56-5 (SUC(+) clone) growing from sucrose, mixture of glucose and fructose, glucose or glycerol was investigated...
  14. Song Y, Choi M, Park J, Kim M, Kim E, Kang H, et al. Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans. Appl Environ Microbiol. 2007;73:4446-54 pubmed
    In an attempt to engineer a Yarrowia lipolytica strain to produce glycoproteins lacking the outer-chain mannose residues of N-linked oligosaccharides, we investigated the functions of the OCH1 gene encoding a putative alpha-1,6-..
  15. Jiménez Bremont J, Ruiz Herrera J. Analysis of the transcriptional regulation of YlODC gene from the dimorphic fungus Yarrowia lipolytica. Microbiol Res. 2008;163:717-23 pubmed publisher
    ..In the present report, the transcriptional regulation of the gene encoding ODC from the dimorphic fungus Yarrowia lipolytica (YlODC) was analyzed...
  16. Jardón R, Gancedo C, Flores C. The gluconeogenic enzyme fructose-1,6-bisphosphatase is dispensable for growth of the yeast Yarrowia lipolytica in gluconeogenic substrates. Eukaryot Cell. 2008;7:1742-9 pubmed publisher
    The genes encoding gluconeogenic enzymes in the nonconventional yeast Yarrowia lipolytica were found to be differentially regulated. The expression of Y...
  17. Gaillardin C, Neuvéglise C, Kerscher S, Nicaud J. Mitochondrial genomes of yeasts of the Yarrowia clade. FEMS Yeast Res. 2012;12:317-31 pubmed publisher
    ..deformans, Candida galli, and Candida phangngensis have been recently reported to be the close relatives of Yarrowia lipolytica...
  18. Tai M, Stephanopoulos G. Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production. Metab Eng. 2013;15:1-9 pubmed publisher
    ..To this end, we have engineered the oleaginous yeast Yarrowia lipolytica...
  19. Holz M, Otto C, Kretzschmar A, Yovkova V, Aurich A, Pötter M, et al. Overexpression of alpha-ketoglutarate dehydrogenase in Yarrowia lipolytica and its effect on production of organic acids. Appl Microbiol Biotechnol. 2011;89:1519-26 pubmed publisher
    The yeast Yarrowia lipolytica is one of the most intensively studied "non-conventional" yeast species...
  20. Lopes M, Gomes N, Mota M, Belo I. Yarrowia lipolytica growth under increased air pressure: influence on enzyme production. Appl Biochem Biotechnol. 2009;159:46-53 pubmed publisher
    ..In this work, a pressurized bioreactor was used for Yarrowia lipolytica batch cultivation under increased air pressure from 1 to 6 bar...
  21. Holz M, Förster A, Mauersberger S, Barth G. Aconitase overexpression changes the product ratio of citric acid production by Yarrowia lipolytica. Appl Microbiol Biotechnol. 2009;81:1087-96 pubmed publisher
    The yeast Yarrowia lipolytica secretes high amounts of various organic acids, like citric acid (CA) and isocitric acid (ICA) under an excess of carbon source and several conditions of growth limitation...
  22. Park J, Song Y, Cheon S, Kwon O, Oh D, Jigami Y, et al. Essential role of YlMPO1, a novel Yarrowia lipolytica homologue of Saccharomyces cerevisiae MNN4, in mannosylphosphorylation of N- and O-linked glycans. Appl Environ Microbiol. 2011;77:1187-95 pubmed publisher
    ..To identify genes relevant to mannosylphosphorylation in the dimorphic yeast Yarrowia lipolytica, the molecular functions of five Y. lipolytica genes showing significant sequence homology with S...
  23. Kabran P, Rossignol T, Gaillardin C, Nicaud J, Neuv glise C. Alternative splicing regulates targeting of malate dehydrogenase in Yarrowia lipolytica. DNA Res. 2012;19:231-44 pubmed publisher
    ..We report the first case of AS with functional consequences in the yeast Yarrowia lipolytica...
  24. Galkin A, Dröse S, Brandt U. The proton pumping stoichiometry of purified mitochondrial complex I reconstituted into proteoliposomes. Biochim Biophys Acta. 2006;1757:1575-81 pubmed
    ..Here we determined the pumping stoichiometry of mitochondrial complex I from the strictly aerobic yeast Yarrowia lipolytica. With intact mitochondria, the measured value of 3...
  25. Mansour S, Bailly J, Delettre J, Bonnarme P. A proteomic and transcriptomic view of amino acids catabolism in the yeast Yarrowia lipolytica. Proteomics. 2009;9:4714-25 pubmed publisher
    The yeast Yarrowia lipolytica has to develop dynamic metabolic adaptation mechanisms to survive within the cheese habitat...
  26. Rywińska A, Juszczyk P, Wojtatowicz M, Rymowicz W. Chemostat study of citric acid production from glycerol by Yarrowia lipolytica. J Biotechnol. 2011;152:54-7 pubmed publisher
    ..rate and the chemical composition of the production medium impacts on the synthesis of citric acid by the Yarrowia lipolytica strain Wratislavia AWG7 from glycerol in a chemostat culture. The yeast Y...
  27. Moeller L, Zehnsdorf A, Aurich A, Bley T, Strehlitz B. Substrate utilization by recombinant Yarrowia lipolytica growing on sucrose. Appl Microbiol Biotechnol. 2012;93:1695-702 pubmed publisher
    We report the study of the dynamics of substrate utilization by the genetic modified strain Yarrowia lipolytica H222-S4(p67ICL1) T5...
  28. Theerachat M, Emond S, Cambon E, Bordes F, Marty A, Nicaud J, et al. Engineering and production of laccase from Trametes versicolor in the yeast Yarrowia lipolytica. Bioresour Technol. 2012;125:267-74 pubmed publisher
    The lcc1 gene coding for the laccase from Trametes versicolor DSM11269 was cloned into the genome of Yarrowia lipolytica using either single or multiple integration sites...
  29. Ali S, Shultz J, Ikram Ul Haq -. High performance microbiological transformation of L-tyrosine to L-dopa by Yarrowia lipolytica NRRL-143. BMC Biotechnol. 2007;7:50 pubmed
    ..Pre-grown cells of Yarrowia lipolytica NRRL-143 were used for the microbiological transformation of L-tyrosine to L-dopa...
  30. Aloulou A, Puccinelli D, De Caro A, Leblond Y, Carriere F. A comparative study on two fungal lipases from Thermomyces lanuginosus and Yarrowia lipolytica shows the combined effects of detergents and pH on lipase adsorption and activity. Biochim Biophys Acta. 2007;1771:1446-56 pubmed
    The effects of various detergents and pH on the interfacial binding and activity of two fungal lipases from Yarrowia lipolytica (YLLIP2) and Thermomyces lanuginosus (TLL) were investigated using trioctanoin emulsions as well as ..
  31. Sakai Y, Oku M, van der Klei I, Kiel J. Pexophagy: autophagic degradation of peroxisomes. Biochim Biophys Acta. 2006;1763:1767-75 pubmed
    ..Subsequently, the engulfed peroxisome cluster is degraded. This review discusses the current state of knowledge of pexophagy with emphasis on studies on methylotrophic yeast species. ..
  32. Cholet O, Hénaut A, Hébert A, Bonnarme P. Transcriptional analysis of L-methionine catabolism in the cheese-ripening yeast Yarrowia lipolytica in relation to volatile sulfur compound biosynthesis. Appl Environ Microbiol. 2008;74:3356-67 pubmed publisher
    b>Yarrowia lipolytica is one of the yeasts most frequently isolated from the surface of ripened cheeses...
  33. Zhou J, Yin X, Madzak C, Du G, Chen J. Enhanced ?-ketoglutarate production in Yarrowia lipolytica WSH-Z06 by alteration of the acetyl-CoA metabolism. J Biotechnol. 2012;161:257-64 pubmed publisher
    ..In previous research, a thiamine-auxotrophic yeast for ?-KG overproduction was screened and named as Yarrowia lipolytica WSH-Z06...
  34. Yu M, Lange S, Richter S, Tan T, Schmid R. High-level expression of extracellular lipase Lip2 from Yarrowia lipolytica in Pichia pastoris and its purification and characterization. Protein Expr Purif. 2007;53:255-63 pubmed
    The extracellular lipase gene from Yarrowia lipolytica (YlLip2) was cloned into the pPICZalphaA and integrated into the genome of the methylotrophic yeast Pichia pastoris X-33...
  35. Jiang Z, Song H, Gupta N, Ma L, Wu Z. Cell surface display of functionally active lipases from Yarrowia lipolytica in Pichia pastoris. Protein Expr Purif. 2007;56:35-9 pubmed
    The lipase genes of Yarrowia lipolytica, LIPY7 and LIPY8, fused with FLO-flocculation domain sequence from Saccharomyces cerevisiae at their N-termini, were expressed in Pichia pastoris KM71...
  36. Roth R, Moodley V, van Zyl P. Heterologous expression and optimized production of an Aspergillus aculeatus endo-1,4-beta-mannanase in Yarrowia lipolytica. Mol Biotechnol. 2009;43:112-20 pubmed publisher
    ..was cloned and expressed in the promising heterologous enzyme producer, the ascomycetous yeast Yarrowia lipolytica...
  37. Liu W, Pan X, Jia B, Zhao H, Xu L, Liu Y, et al. Surface display of active lipases Lip7 and Lip8 from Yarrowia lipolytica on Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2010;88:885-91 pubmed publisher
    ..The genes encoding Yarrowia lipolytica lipases Lip7 and Lip8 were fused with the gene encoding small binding subunit Aga2 of a-agglutinin...
  38. Yadav K, Adsul M, Bastawde K, Jadhav D, Thulasiram H, Gokhale D. Differential induction, purification and characterization of cold active lipase from Yarrowia lipolytica NCIM 3639. Bioresour Technol. 2011;102:10663-70 pubmed publisher
    The production, purification and characterization of cold active lipases by Yarrowia lipolytica NCIM 3639 is described...
  39. Duquesne S, Bordes F, Fudalej F, Nicaud J, Marty A. The yeast Yarrowia lipolytica as a generic tool for molecular evolution of enzymes. Methods Mol Biol. 2012;861:301-12 pubmed publisher
    It has been 20 years since strains of the yeast Yarrowia lipolytica were developed for the expression of recombinant proteins as alternative host to the commonly used yeasts, Pichia pastoris and Saccharomyces cerevisiae...
  40. Otto C, Yovkova V, Aurich A, Mauersberger S, Barth G. Variation of the by-product spectrum during ?-ketoglutaric acid production from raw glycerol by overexpression of fumarase and pyruvate carboxylase genes in Yarrowia lipolytica. Appl Microbiol Biotechnol. 2012;95:905-17 pubmed publisher
    The yeast Yarrowia lipolytica secretes high amounts of various organic acids, like citric, isocitric, pyruvic (PA), and ?-ketoglutaric (KGA) acids, triggered by growth limitation and excess of carbon source...
  41. De Pourcq K, Tiels P, Van Hecke A, Geysens S, Vervecken W, Callewaert N. Engineering Yarrowia lipolytica to produce glycoproteins homogeneously modified with the universal Man3GlcNAc2 N-glycan core. PLoS ONE. 2012;7:e39976 pubmed publisher
    b>Yarrowia lipolytica is a dimorphic yeast that efficiently secretes various heterologous proteins and is classified as "generally recognized as safe." Therefore, it is an attractive protein production host...
  42. Wang Z, Xu H, Wang G, Chi Z, Chi Z. Disruption of the MIG1 gene enhances lipid biosynthesis in the oleaginous yeast Yarrowia lipolytica ACA-DC 50109. Biochim Biophys Acta. 2013;1831:675-82 pubmed publisher
    In this study, the MIG1 gene in the oleaginous yeast Yarrowia lipolytica ACA-DC 50109 (the parent yeast) was disrupted and the disruptant M25 obtained could grow in yeast nitrogen base-N5000 medium without uracil or the medium with 2-..
  43. Guerrero Castillo S, Vázquez Acevedo M, Gonzalez Halphen D, Uribe Carvajal S. In Yarrowia lipolytica mitochondria, the alternative NADH dehydrogenase interacts specifically with the cytochrome complexes of the classic respiratory pathway. Biochim Biophys Acta. 2009;1787:75-85 pubmed publisher
    In Yarrowia lipolytica, mitochondria contain a branched respiratory chain constituted by the classic complexes I, II, III and IV, plus an alternative external NADH dehydrogenase (NDH2e) and an alternative oxidase (AOX)...
  44. Dröse S, Galkin A, Brandt U. Chapter 26 Measurement of superoxide formation by mitochondrial complex I of Yarrowia lipolytica. Methods Enzymol. 2009;456:475-90 pubmed publisher
    ..Mitochondrial membranes from the obligate aerobic yeast Yarrowia lipolytica, as well as the purified and reconstituted enzyme, can be used to measure complex I-dependent ..
  45. Desfougères T, Haddouche R, Fudalej F, Neuvéglise C, Nicaud J. SOA genes encode proteins controlling lipase expression in response to triacylglycerol utilization in the yeast Yarrowia lipolytica. FEMS Yeast Res. 2010;10:93-103 pubmed publisher
    The oleaginous yeast Yarrowia lipolytica efficiently metabolizes hydrophobic substrates such as alkanes, fatty acids or triacylglycerol. This yeast has been identified in oil-polluted water and in lipid-rich food...
  46. Zhou J, Zhou H, Du G, Liu L, Chen J. Screening of a thiamine-auxotrophic yeast for alpha-ketoglutaric acid overproduction. Lett Appl Microbiol. 2010;51:264-71 pubmed publisher
    ..The strain was identified as Yarrowia lipolytica based on physiological, chemical, and phylogenetic analysis...
  47. Boonvitthya N, Bozonnet S, Burapatana V, O Donohue M, Chulalaksananukul W. Comparison of the heterologous expression of Trichoderma reesei endoglucanase II and cellobiohydrolase II in the yeasts Pichia pastoris and Yarrowia lipolytica. Mol Biotechnol. 2013;54:158-69 pubmed publisher
    ..II and cellobiohydrolase II from the fungus Trichoderma reesei QM9414 were successfully cloned and expressed in Yarrowia lipolytica under the control of the POX2 or TEF promoters, and using either the native or preproLip2 secretion ..
  48. Dröse S, Galkin A, Brandt U. Proton pumping by complex I (NADH:ubiquinone oxidoreductase) from Yarrowia lipolytica reconstituted into proteoliposomes. Biochim Biophys Acta. 2005;1710:87-95 pubmed
    ..While this study was not specifically designed to demonstrate possible additional sodium translocating properties of complex I, we did not find indications for primary or secondary Na+ translocation by Y. lipolytica complex I. ..
  49. Thevenieau F, Le Dall M, Nthangeni B, Mauersberger S, Marchal R, Nicaud J. Characterization of Yarrowia lipolytica mutants affected in hydrophobic substrate utilization. Fungal Genet Biol. 2007;44:531-42 pubmed
    ..affected in these pathways were generated by random insertion of a mutagenesis cassette MTC into the genome of Yarrowia lipolytica. About 9...
  50. Clason T, Zickermann V, Ruiz T, Brandt U, Radermacher M. Direct localization of the 51 and 24 kDa subunits of mitochondrial complex I by three-dimensional difference imaging. J Struct Biol. 2007;159:433-42 pubmed
    ..We have determined the 3D structure of complex I from Yarrowia lipolytica lacking the flavoprotein part of the N-module, which consists of the 51 kDa (NUBM) and the 24 kDa (..
  51. Tocilescu M, Fendel U, Zwicker K, Kerscher S, Brandt U. Exploring the ubiquinone binding cavity of respiratory complex I. J Biol Chem. 2007;282:29514-20 pubmed
    ..the proposed ubiquinone binding cavity of complex I by site-directed mutagenesis in the strictly aerobic yeast Yarrowia lipolytica...
  52. Rymowicz W, Fatykhova A, Kamzolova S, Rywińska A, Morgunov I. Citric acid production from glycerol-containing waste of biodiesel industry by Yarrowia lipolytica in batch, repeated batch, and cell recycle regimes. Appl Microbiol Biotechnol. 2010;87:971-9 pubmed publisher
    b>Yarrowia lipolytica A-101-1.22 produces high citric acid (112 g l(-1)) with a yield of 0.6 g g(-1) and a productivity of 0.71 g l(-1) h(-1) during batch cultivation in the medium with glycerol-containing waste of biodiesel industry...
  53. Hunte C, Zickermann V, Brandt U. Functional modules and structural basis of conformational coupling in mitochondrial complex I. Science. 2010;329:448-51 pubmed publisher
    ..We suggest that a approximately 60-angstrom-long helical transmission element is critical for transducing conformational energy to proton-pumping elements in the distal module of the membrane arm. ..
  54. Zhang B, Rong C, Chen H, Song Y, Zhang H, Chen W. De novo synthesis of trans-10, cis-12 conjugated linoleic acid in oleaginous yeast Yarrowia lipolytica. Microb Cell Fact. 2012;11:51 pubmed publisher
    ..The linoleic acid isomerase gene from Propionibacterium acnes was expressed in Yarrowia lipolytica Polh...
  55. Shkumatov V, Frolova N, Rudaia E, Faletrov I, Mauerberger S, Barth G. [Range of substrates and steroid bioconversion reactions performed by recombinant microorganisms Saccharomyces cerevisiae and Yarrowia lipolytica expressing cytochrome P450c17]. Prikl Biokhim Mikrobiol. 2006;42:539-46 pubmed
    ..of progesterone and some of its derivatives was studied in yeast strains Saccharomyces cerevisiae YEp51alpha, Yarrowia lipolytica E129A15, and expressing cytochrome P450c17...
  56. Turki S, Ayed A, Chalghoumi N, Weekers F, Thonart P, Kallel H. An enhanced process for the production of a highly purified extracellular lipase in the non-conventional yeast Yarrowia lipolytica. Appl Biochem Biotechnol. 2010;160:1371-85 pubmed publisher
    b>Yarrowia lipolytica LgX64.81 is a non-genetically modified mutant that was previously identified as a promising microorganism for extracellular lipase production...
  57. Oh M, Cheon S, Kang H, Kim J. Functional characterization of the unconventional splicing of Yarrowia lipolytica HAC1 mRNA induced by unfolded protein response. Yeast. 2010;27:443-52 pubmed publisher
    ..In this study, we isolated and characterized a Yarrowia lipolytica HAC1 (YlHAC1) encoding a basic-leucine zipper transcription factor...
  58. Wang X, Sun Y, Shen X, Ke F, Zhao H, Liu Y, et al. Intracellular expression of Vitreoscilla hemoglobin improves production of Yarrowia lipolytica lipase LIP2 in a recombinant Pichia pastoris. Enzyme Microb Technol. 2012;50:22-8 pubmed publisher
    The Yarrowia lipolytica lipase LIP2 (YlLIP2) gene lip2 and Vitreoscilla hemoglobin gene vgb were co-expressed in Pichia pastoris, both under the control of AOX1 promoter, in order to alleviate respiration limitation under conditions of ..
  59. Ogrydziak D, Nicaud J. Characterization of Yarrowia lipolytica XPR2 multi-copy strains over-producing alkaline extracellular protease - a system for rapidly increasing secretory pathway cargo loads. FEMS Yeast Res. 2012;12:938-48 pubmed publisher
    ..They should provide an excellent system for kinetic analysis of gene expression responses to acute increases in secretory pathway cargo load. ..
  60. Fickers P, Fudalej F, Le Dall M, Casaregola S, Gaillardin C, Thonart P, et al. Identification and characterisation of LIP7 and LIP8 genes encoding two extracellular triacylglycerol lipases in the yeast Yarrowia lipolytica. Fungal Genet Biol. 2005;42:264-74 pubmed publisher
    In the lipolytic yeast Yarrowia lipolytica, the LIP2 gene was previously reported to encode an extracellular lipase...
  61. Boukh Viner T, Guo T, Alexandrian A, Cerracchio A, Gregg C, Haile S, et al. Dynamic ergosterol- and ceramide-rich domains in the peroxisomal membrane serve as an organizing platform for peroxisome fusion. J Cell Biol. 2005;168:761-73 pubmed
    ..Our data suggest a model for the multistep reorganization of the multicomponent peroxisome fusion machinery that transiently associates with ECR domains. ..
  62. Makri A, Fakas S, Aggelis G. Metabolic activities of biotechnological interest in Yarrowia lipolytica grown on glycerol in repeated batch cultures. Bioresour Technol. 2010;101:2351-8 pubmed publisher
    The growth of Yarrowia lipolytica on glycerol was studied in bioreactor repeated batch cultures and three distinct phases, namely biomass production phase, lipogenic phase and citric acid production phase were identified during growth ..
  63. Darvishi F, Destain J, Nahvi I, Thonart P, Zarkesh Esfahani H. High-level production of extracellular lipase by Yarrowia lipolytica mutants from methyl oleate. N Biotechnol. 2011;28:756-60 pubmed publisher
    The yeast Yarrowia lipolytica degrades efficiently low-cost hydrophobic substrates for the production of various added-value products such as lipases. To obtain yeast strains producing high levels of extracellular lipase, Y...
  64. Kerscher S, Grgic L, Garofano A, Brandt U. Application of the yeast Yarrowia lipolytica as a model to analyse human pathogenic mutations in mitochondrial complex I (NADH:ubiquinone oxidoreductase). Biochim Biophys Acta. 2004;1659:197-205 pubmed
    ..this dilemma for the largest and therefore most affected respiratory chain enzyme, we have established the yeast Yarrowia lipolytica as a eucaryotic model system to analyse respiratory chain complex I...
  65. Aloulou A, Rodriguez J, Puccinelli D, Mouz N, Leclaire J, Leblond Y, et al. Purification and biochemical characterization of the LIP2 lipase from Yarrowia lipolytica. Biochim Biophys Acta. 2007;1771:228-37 pubmed
    The LIP2 lipase from the yeast Yarrowia lipolytica (YLLIP2) was obtained from two genetically modified strains with multi-copies of the lip2 gene and further purified using gel filtration and cation exchange chromatography...
  66. Förster A, Aurich A, Mauersberger S, Barth G. Citric acid production from sucrose using a recombinant strain of the yeast Yarrowia lipolytica. Appl Microbiol Biotechnol. 2007;75:1409-17 pubmed
    The yeast Yarrowia lipolytica is able to secrete high amounts of several organic acids under conditions of growth limitation and carbon source excess...
  67. Lasserre J, Nicaud J, Pagot Y, Joubert Caron R, Caron M, Hardouin J. First complexomic study of alkane-binding protein complexes in the yeast Yarrowia lipolytica. Talanta. 2010;80:1576-85 pubmed publisher
    The yeast Yarrowia lipolytica uses hydrophobic substrates, such as alkanes, fatty acids and oils, for its growth...
  68. Yang C, Huang Y, Chen C, Wen C. Heterologous expression of Thermobifida fusca thermostable alpha-amylase in Yarrowia lipolytica and its application in boiling stable resistant sago starch preparation. J Ind Microbiol Biotechnol. 2010;37:953-60 pubmed publisher
    ..the thermostable alpha-amylase in Thermobifida fusca NTU22 was amplified by PCR, sequenced, and cloned into Yarrowia lipolytica P01g host strain using the vector pYLSC1 allowing constitutive expression and secretion of the protein...
  69. Fickers P, Fudalej F, Nicaud J, Destain J, Thonart P. Selection of new over-producing derivatives for the improvement of extracellular lipase production by the non-conventional yeast Yarrowia lipolytica. J Biotechnol. 2005;115:379-86 pubmed
    The non-conventional yeast Yarrowia lipolytica produces an extracellular lipase encoded by the LIP2 gene. Mutant strains with enhanced productivity were previously obtained either by chemical mutagenesis or genetic engineering...
  70. Song H, Jiang Z, Ma L. Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216. Protein Expr Purif. 2006;47:393-7 pubmed
    We isolated two lipase genes LIPY7, LIPY8 from Yarrowia lipolytica CGMCC (China general microbiological culture collection center) AS 2.1216. The LIPY7 and LIPY8 genes encode a 366 and a 371-amino acid protein, respectively...
  71. Bankar A, Kumar A, Zinjarde S. Removal of chromium (VI) ions from aqueous solution by adsorption onto two marine isolates of Yarrowia lipolytica. J Hazard Mater. 2009;170:487-94 pubmed publisher
    The removal of chromium (VI) ions from aqueous solutions by the biomass of two marine strains of Yarrowia lipolytica (NCIM 3589 and 3590) was studied with respect to pH, temperature, biomass, sea salt concentration, agitation speed, ..
  72. Fontanille P, Kumar V, Christophe G, Nouaille R, Larroche C. Bioconversion of volatile fatty acids into lipids by the oleaginous yeast Yarrowia lipolytica. Bioresour Technol. 2012;114:443-9 pubmed publisher
    The valorization of volatile fatty acids into microbial lipids by the oleaginous yeast Yarrowia lipolytica was investigated...
  73. Morales Vargas A, Dominguez A, Ruiz Herrera J. Identification of dimorphism-involved genes of Yarrowia lipolytica by means of microarray analysis. Res Microbiol. 2012;163:378-87 pubmed publisher
    ..microarrays, in this work we identified genes involved in the early stages of the yeast-to-mycelium transition of Yarrowia lipolytica induced by a shift in pH of the medium...
  74. Morgunov I, Solodovnikova N, Sharyshev A, Kamzolova S, Finogenova T. Regulation of NAD(+)-dependent isocitrate dehydrogenase in the citrate producing yeast Yarrowia lipolytica. Biochemistry (Mosc). 2004;69:1391-8 pubmed
    The mechanism of the increased accumulation (overproduction) of citric acids in the yeast Yarrowia lipolytica while growing in the presence of glucose under nitrogen deficiency was investigated...
  75. Maly T, Grgic L, Zwicker K, Zickermann V, Brandt U, Prisner T. Cluster N1 of complex I from Yarrowia lipolytica studied by pulsed EPR spectroscopy. J Biol Inorg Chem. 2006;11:343-50 pubmed
    ..adenine dinucleotide (NADH), NADH:ubiquinone oxidoreductase (complex I) of the strictly aerobic yeast Yarrowia lipolytica shows clear signals from five different paramagnetic iron-sulfur (FeS) clusters (N1-N5) which can be ..
  76. Chang J, Fagarasanu A, Rachubinski R. Peroxisomal peripheral membrane protein YlInp1p is required for peroxisome inheritance and influences the dimorphic transition in the yeast Yarrowia lipolytica. Eukaryot Cell. 2007;6:1528-37 pubmed
    ..We have analyzed peroxisome dynamics and inheritance in the dimorphic yeast Yarrowia lipolytica. Most peroxisomes are anchored at the periphery of cells of Y. lipolytica...
  77. Zhao C, Cui W, Liu X, Chi Z, Madzak C. Expression of inulinase gene in the oleaginous yeast Yarrowia lipolytica and single cell oil production from inulin-containing materials. Metab Eng. 2010;12:510-7 pubmed publisher
    b>Yarrowia lipolytica ACA-DC 50109 has been reported to be an oleaginous yeast and significant quantities of lipids were accumulated inside the yeast cells...
  78. Yuzbasheva E, Yuzbashev T, Laptev I, Konstantinova T, Sineoky S. Efficient cell surface display of Lip2 lipase using C-domains of glycosylphosphatidylinositol-anchored cell wall proteins of Yarrowia lipolytica. Appl Microbiol Biotechnol. 2011;91:645-54 pubmed publisher
    ..In this study, we have focused on the cell wall immobilization of Yarrowia lipolytica Lip2 protein--an enzyme that has a wide technological application. By genome analysis of Y...
  79. Beopoulos A, Haddouche R, Kabran P, Dulermo T, Chardot T, Nicaud J. Identification and characterization of DGA2, an acyltransferase of the DGAT1 acyl-CoA:diacylglycerol acyltransferase family in the oleaginous yeast Yarrowia lipolytica. New insights into the storage lipid metabolism of oleaginous yeasts. Appl Microbiol Biotechnol. 2012;93:1523-37 pubmed publisher
    ..In the lipid accumulation-oriented metabolism of the oleaginous yeast Yarrowia lipolytica, storage lipids are mostly found in the form of TAG, and only small amounts of SE accumulate...
  80. Cervantes Chávez J, Ruiz Herrera J. The regulatory subunit of protein kinase A promotes hyphal growth and plays an essential role in Yarrowia lipolytica. FEMS Yeast Res. 2007;7:929-40 pubmed
    The gene encoding the regulatory subunit (RKA1) of the cAMP-dependent protein kinase (PKA) of Yarrowia lipolytica was isolated to analyze the role of the PKA pathway in the dimorphic transition of the fungus...
  81. Fickers P, Benetti P, Wache Y, Marty A, Mauersberger S, Smit M, et al. Hydrophobic substrate utilisation by the yeast Yarrowia lipolytica, and its potential applications. FEMS Yeast Res. 2005;5:527-43 pubmed
    The alkane-assimilating yeast Yarrowia lipolytica degrades very efficiently hydrophobic substrates such as n-alkanes, fatty acids, fats and oils for which it has specific metabolic pathways...
  82. Babour A, Kabani M, Boisramé A, Beckerich J. Characterization of Ire1 in the yeast Yarrowia lipolytica reveals an important role for the Sls1 nucleotide exchange factor in unfolded protein response regulation. Curr Genet. 2008;53:337-46 pubmed publisher
    ..Here, we report on the characterization of the Yarrowia lipolytica Ire1 ortholog...
  83. Maharajh D, Lalloo R, Görgens J. Effect of an exponential feeding regime on the production of Rhodotorula araucariae epoxide hydrolase in Yarrowia lipolytica. Lett Appl Microbiol. 2008;47:520-5 pubmed publisher
    To evaluate the effect of and exponential feeding regime on the production of epoxide hydrolase (EH) enzyme in recombinant Yarrowia lipolytica in comparison to a constant feed strategy.
  84. Bordes F, Barbe S, Escalier P, Mourey L, André I, Marty A, et al. Exploring the conformational states and rearrangements of Yarrowia lipolytica Lipase. Biophys J. 2010;99:2225-34 pubmed publisher
    We report the 1.7 Å resolution crystal structure of the Lip2 lipase from Yarrowia lipolytica in its closed conformation...
  85. Athenstaedt K. YALI0E32769g (DGA1) and YALI0E16797g (LRO1) encode major triacylglycerol synthases of the oleaginous yeast Yarrowia lipolytica. Biochim Biophys Acta. 2011;1811:587-96 pubmed publisher
    The oleaginous yeast Yarrowia lipolytica has an outstanding capacity to produce and store triacylglycerols resembling adipocytes of higher eukaryotes...
  86. Wang X, Sun Y, Ke F, Zhao H, Liu T, Xu L, et al. Constitutive expression of Yarrowia lipolytica lipase LIP2 in Pichia pastoris using GAP as promoter. Appl Biochem Biotechnol. 2012;166:1355-67 pubmed publisher
    A gene encoding Yarrowia lipolytica lipase LIP2 (YlLIP2) was cloned into a constitutive expression vector pGAPZ?A and electrotransformed into the Pichia pastoris X-33 strain...
  87. Loira N, Dulermo T, Nicaud J, Sherman D. A genome-scale metabolic model of the lipid-accumulating yeast Yarrowia lipolytica. BMC Syst Biol. 2012;6:35 pubmed publisher
    b>Yarrowia lipolytica is an oleaginous yeast which has emerged as an important microorganism for several biotechnological processes, such as the production of organic acids, lipases and proteases...
  88. Radermacher M, Ruiz T, Clason T, Benjamin S, Brandt U, Zickermann V. The three-dimensional structure of complex I from Yarrowia lipolytica: a highly dynamic enzyme. J Struct Biol. 2006;154:269-79 pubmed
    The structure of complex I from Yarrowia lipolytica was determined by three-dimensional electron microscopy. A random conical data set was collected from deep stain embedded particles. More than 14000 image pairs were analyzed...
  89. Bankar A, Kumar A, Zinjarde S. Environmental and industrial applications of Yarrowia lipolytica. Appl Microbiol Biotechnol. 2009;84:847-65 pubmed publisher
    b>Yarrowia lipolytica is a fungus that degrades hydrophobic substrates very efficiently...
  90. Huang Y, Chen Y, Chen C, Chen W, Ciou Y, Liu W, et al. Production of ferulic acid from lignocellulolytic agricultural biomass by Thermobifida fusca thermostable esterase produced in Yarrowia lipolytica transformant. Bioresour Technol. 2011;102:8117-22 pubmed publisher
    A gene (axe) encoding the AXE thermostable esterase in Thermobifida fusca NTU22 was cloned into a Yarrowia lipolytica P01g host strain. Recombinant expression resulted in extracellular esterase production at levels as high as 70...
  91. Tomaszewska L, Rywińska A, Gładkowski W. Production of erythritol and mannitol by Yarrowia lipolytica yeast in media containing glycerol. J Ind Microbiol Biotechnol. 2012;39:1333-43 pubmed publisher
    ..In a shake-flasks experiment in a buffered medium, nine Yarrowia lipolytica strains were examined for polyols production...
  92. Jolivalt C, Madzak C, Brault A, Caminade E, Malosse C, Mougin C. Expression of laccase IIIb from the white-rot fungus Trametes versicolor in the yeast Yarrowia lipolytica for environmental applications. Appl Microbiol Biotechnol. 2005;66:450-6 pubmed
    ..We developed the heterologous expression of a laccase from the white-rot fungus Trametes versicolor in the yeast Yarrowia lipolytica as a first step for enzyme evolution...