kluyveromyces

Summary

Summary: An ascomycetous yeast of the fungal family Saccharomycetaceae, order SACCHAROMYCETALES.

Top Publications

  1. Qian J, Qin X, Yin Q, Chu J, Wang Y. Cloning and characterization of Kluyveromyces marxianus Hog1 gene. Biotechnol Lett. 2011;33:571-5 pubmed publisher
    The mitogen-activated protein kinase Hog1 gene (Kmhog1) was isolated from Kluyveromyces marxianus strain NBRC 1777 by degenerate PCR and genome walking, and then disrupted to construct a mutant strain hog1?...
  2. Limtong S, Sringiew C, Yongmanitchai W. Production of fuel ethanol at high temperature from sugar cane juice by a newly isolated Kluyveromyces marxianus. Bioresour Technol. 2007;98:3367-74 pubmed
    b>Kluyveromyces marxianus DMKU 3-1042, isolated by an enrichment technique in a sugar cane juice medium supplemented with 4% (w/v) ethanol at 35 degrees C, produced high concentrations of ethanol at both 40 and 45 degrees C...
  3. Xu J, McEachern M. Maintenance of very long telomeres by recombination in the Kluyveromyces lactis stn1-M1 mutant involves extreme telomeric turnover, telomeric circles, and concerted telomeric amplification. Mol Cell Biol. 2012;32:2992-3008 pubmed publisher
    ..Here, we studied the recombinational telomere elongation (RTE) of the Kluyveromyces lactis stn1-M1 mutant...
  4. Moreno A, Ibarra D, Ballesteros I, Gonzalez A, Ballesteros M. Comparing cell viability and ethanol fermentation of the thermotolerant yeast Kluyveromyces marxianus and Saccharomyces cerevisiae on steam-exploded biomass treated with laccase. Bioresour Technol. 2013;135:239-45 pubmed publisher
    In this study, the thermotolerant yeast Kluyveromyces marxianus CECT 10875 was compared to the industrial strain Saccharomyces cerevisiae Ethanol Red for lignocellulosic ethanol production...
  5. Staneva D, Peycheva E, Georgieva M, Efremov T, Miloshev G. Application of comet assay for the assessment of DNA damage caused by chemical genotoxins in the dairy yeast Kluyveromyces lactis. Antonie Van Leeuwenhoek. 2013;103:143-52 pubmed publisher
    b>Kluyveromyces lactis, also known as dairy yeast, has numerous applications in scientific research and practice...
  6. Backhaus K, Buchwald U, Heppeler N, Schmitz H, Rodicio R, Heinisch J. Milk and sugar: regulation of cell wall synthesis in the milk yeast Kluyveromyces lactis. Eur J Cell Biol. 2011;90:745-50 pubmed publisher
    The milk yeast Kluyveromyces lactis is an alternative model yeast to the well established Saccharomyces cerevisiae. The cell wall of these fungi consists of polysaccharides (i.e...
  7. Maccaferri S, Klinder A, Brigidi P, Cavina P, Costabile A. Potential probiotic Kluyveromyces marxianus B0399 modulates the immune response in Caco-2 cells and peripheral blood mononuclear cells and impacts the human gut microbiota in an in vitro colonic model system. Appl Environ Microbiol. 2012;78:956-64 pubmed publisher
    ..probiotics, the aim of this study was to broadly investigate the beneficial properties of the lactic yeast Kluyveromyces marxianus (formerly Kluyveromyces fragilis) B0399. Several potential probiotic traits of K...
  8. Naumov G. Identification of the lactose LAC gene superfamilies in Kluyveromyces yeast. Dokl Biochem Biophys. 2008;420:158-60 pubmed
  9. Rocha S, Abrahão Neto J, Cerdán M, González Siso M, Gombert A. Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus. Microb Cell Fact. 2010;9:4 pubmed publisher
    In spite of its advantageous physiological properties for bioprocess applications, the use of the yeast Kluyveromyces marxianus as a host for heterologous protein production has been very limited, in constrast to its close relative ..

More Information

Publications119 found, 100 shown here

  1. Eiadpum A, Limtong S, Phisalaphong M. High-temperature ethanol fermentation by immobilized coculture of Kluyveromyces marxianus and Saccharomyces cerevisiae. J Biosci Bioeng. 2012;114:325-9 pubmed publisher
    Suspended and immobilized cocultures of the thermotolerant yeast, Kluyveromyces marxianus DMKU 3-1042 and the mesophilic flocculent yeast, Saccharomyces cerevisiae M30 were studied for their abilities to improve production and stability ..
  2. Heinisch J, Buchwald U, Gottschlich A, Heppeler N, Rodicio R. A tool kit for molecular genetics of Kluyveromyces lactis comprising a congenic strain series and a set of versatile vectors. FEMS Yeast Res. 2010;10:333-42 pubmed publisher
    A set of different marker deletions starting with a ura3 derivative of the Kluyveromyces lactis type strain CBS2359 was constructed...
  3. Baruffini E, Goffrini P, Donnini C, Lodi T. Galactose transport in Kluyveromyces lactis: major role of the glucose permease Hgt1. FEMS Yeast Res. 2006;6:1235-42 pubmed publisher
    In Kluyveromyces lactis, galactose transport has been thought to be mediated by the lactose permease encoded by LAC12...
  4. Saliola M, D Amici S, Sponziello M, Mancini P, Tassone P, Falcone C. The transdehydrogenase genes KlNDE1 and KlNDI1 regulate the expression of KlGUT2 in the yeast Kluyveromyces lactis. FEMS Yeast Res. 2010;10:518-26 pubmed publisher
    ..Moreover, both genes seem to be involved in the biogenesis of the mitochondrial tubular network. ..
  5. Jain R, Poulos M, Gros J, Chakravarty A, Shuman S. Substrate specificity and mutational analysis of Kluyveromyces lactis gamma-toxin, a eukaryal tRNA anticodon nuclease. RNA. 2011;17:1336-43 pubmed publisher
    tRNA anticodon damage inflicted by the Kluyveromyces lactis ?-toxin underlies an RNA-based innate immune system that distinguishes self from nonself species...
  6. Serrat M, Rodríguez O, Camacho M, Vallejo J, Ageitos J, Villa T. Influence of nutritional and environmental factors on ethanol and endopolygalacturonase co-production by Kluyveromyces marxianus CCEBI 2011. Int Microbiol. 2011;14:41-9 pubmed
    Ethanol and endopolygalacturonase (endoPG) are simultaneously produced by the yeast Kluyveromyces marxianus CCEBI 2011...
  7. Shefer K, Brown Y, Gorkovoy V, Nussbaum T, Ulyanov N, Tzfati Y. A triple helix within a pseudoknot is a conserved and essential element of telomerase RNA. Mol Cell Biol. 2007;27:2130-43 pubmed
    ..analysis, we propose a three-dimensional model for a pseudoknot in telomerase RNA of the budding yeast Kluyveromyces lactis. Interestingly, this structure includes a U-A.U major-groove triple helix...
  8. Foukis A, Stergiou P, Theodorou L, Papagianni M, Papamichael E. Purification, kinetic characterization and properties of a novel thermo-tolerant extracellular protease from Kluyveromyces marxianus IFO 0288 with potential biotechnological interest. Bioresour Technol. 2012;123:214-20 pubmed publisher
    A novel extracellular hydrolase of ?45kDa molecular mass was purified from Kluyveromyces marxianus IFO 0288 cultures and characterized as serine protease...
  9. Hnatova M, Wésolowski Louvel M, Dieppois G, Deffaud J, Lemaire M. Characterization of KlGRR1 and SMS1 genes, two new elements of the glucose signaling pathway of Kluyveromyces lactis. Eukaryot Cell. 2008;7:1299-308 pubmed publisher
    The expression of the major glucose transporter gene, RAG1, is induced by glucose in Kluyveromyces lactis. This regulation involves several pathways, including one that is similar to Snf3/Rgt2-ScRgt1 in Saccharomyces cerevisiae...
  10. Kim G, Lee J, Jung S, Seo E, Jin S, Kim G, et al. Enzymatic synthesis and characterization of hydroquinone galactoside using Kluyveromyces lactis lactase. J Agric Food Chem. 2010;58:9492-7 pubmed publisher
    ..as a potential skin whitening agent was synthesized by the reaction of lactase (beta-galactosidase) from Kluyveromyces lactis, Aspergillus oryzae, Bacillus circulans, and Thermus sp. with lactose as a donor and HQ as an acceptor...
  11. Saliola M, De Maria I, Lodi T, Fiori A, Falcone C. KlADH3, a gene encoding a mitochondrial alcohol dehydrogenase, affects respiratory metabolism and cytochrome content in Kluyveromyces lactis. FEMS Yeast Res. 2006;6:1184-92 pubmed publisher
    A Kluyveromyces lactis strain, harbouring KlADH3 as the unique alcohol dehydrogenase (ADH) gene, was used in a genetic screen on allyl alcohol to isolate mutants deregulated in the expression of this gene...
  12. Scannell D, Frank A, Conant G, Byrne K, Woolfit M, Wolfe K. Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication. Proc Natl Acad Sci U S A. 2007;104:8397-402 pubmed
    Among yeasts that underwent whole-genome duplication (WGD), Kluyveromyces polysporus represents the lineage most distant from Saccharomyces cerevisiae. By sequencing the K. polysporus genome and comparing it with the S...
  13. Raimondi S, Uccelletti D, Matteuzzi D, Pagnoni U, Rossi M, Palleschi C. Characterization of the superoxide dismutase SOD1 gene of Kluyveromyces marxianus L3 and improved production of SOD activity. Appl Microbiol Biotechnol. 2008;77:1269-77 pubmed
    ..The Cu/Zn superoxide dismutase gene (KmSOD1) of Kluyveromyces marxianus L3 was cloned and characterized...
  14. Matsuzaki C, Nakagawa A, Koyanagi T, Tanaka K, Minami H, Tamaki H, et al. Kluyveromyces marxianus-based platform for direct ethanol fermentation and recovery from cellulosic materials under air-ventilated conditions. J Biosci Bioeng. 2012;113:604-7 pubmed publisher
    ..Running the system under air-supplied conditions, apparently pure ethanol (28g) was recovered from cellobiose (100g) by growing recombinant Kluyveromyces marxianus expressing ?-glucosidase.
  15. Fekete V, Cierna M, Polakova S, Piskur J, Sulo P. Transition of the ability to generate petites in the Saccharomyces/Kluyveromyces complex. FEMS Yeast Res. 2007;7:1237-47 pubmed
    ..was examined after the treatment with ethidium bromide (EB) in over hundred isolates from the Saccharomyces/Kluyveromyces complex. The identity of petite mutants was confirmed by the loss of specific mtDNA DAPI staining patterns...
  16. Pan X, Zhou J, Tian A, Le K, Yuan H, Xue Y, et al. High level expression of a truncated ?-mannanase from alkaliphilic Bacillus sp. N16-5 in Kluyveromyces cicerisporus. Biotechnol Lett. 2011;33:565-70 pubmed publisher
    A truncated alkaline ?-mannanase from alkaliphilic Bacillus sp. N16-5 (MAN330) was expressed and secreted in Kluyveromyces cicerisporus...
  17. Safadi R, Talarek N, Jacques N, Aigle M. Yeast prions: could they be exaptations? The URE2/[URE3] system in Kluyveromyces lactis. FEMS Yeast Res. 2011;11:151-3 pubmed publisher
    We examined aspects of the URE2/[URE3] prion system in Kluyveromyces lactis, which lies on a different evolutionary branch from Saccharomyces. We first analysed the polymorphism of the prion-forming domain in 38 strains...
  18. Conde e Silva N, Gon alves I, Lemaire M, Lesuisse E, Camadro J, Blaiseau P. KlAft, the Kluyveromyces lactis ortholog of Aft1 and Aft2, mediates activation of iron-responsive transcription through the PuCACCC Aft-type sequence. Genetics. 2009;183:93-106 pubmed publisher
    ..Here, we explore regulation of iron homeostasis in the yeast Kluyveromyces lactis that diverged from S. cerevisiae before this event...
  19. Queirós O, Pereira L, Paiva S, Moradas Ferreira P, Casal M. Functional analysis of Kluyveromyces lactis carboxylic acids permeases: heterologous expression of KlJEN1 and KlJEN2 genes. Curr Genet. 2007;51:161-9 pubmed
    ..a detailed physiological and molecular characterization of the mechanisms of transport of carboxylic acids in Kluyveromyces lactis...
  20. Kettner K, Müller E, Otto A, Rödel G, Breunig K, Kriegel T. Identification and characterization of a novel glucose-phosphorylating enzyme in Kluyveromyces lactis. FEMS Yeast Res. 2007;7:683-92 pubmed
    Recent data suggest that hexokinase KlHxk1 (Rag5) represents the only glucose-phosphorylating enzyme of Kluyveromyces lactis, which also is required for glucose signalling. Long-term growth studies of a K...
  21. Kabaha M, Zhitomirsky B, Schwartz I, Tzfati Y. The 5' arm of Kluyveromyces lactis telomerase RNA is critical for telomerase function. Mol Cell Biol. 2008;28:1875-82 pubmed publisher
    ..A binding site for the protein Ku80 is conserved within the 5' arm of TER in Saccharomyces but not in Kluyveromyces budding yeast species...
  22. Abdel Banat B, Nonklang S, Hoshida H, Akada R. Random and targeted gene integrations through the control of non-homologous end joining in the yeast Kluyveromyces marxianus. Yeast. 2010;27:29-39 pubmed publisher
    b>Kluyveromyces marxianus DMKU3-1042 is a thermotolerant yeast strain suitable for high-temperature ethanol fermentation and genetic engineering with linear DNA...
  23. Coulon J, Thouvenin I, Aldeek F, Balan L, Schneider R. Glycosylated quantum dots for the selective labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae yeast strains. J Fluoresc. 2010;20:591-7 pubmed publisher
    ..Results obtained demonstrate that galactose and mannose functionalized QDs associate respectively with Kluyveromyces bulgaricus and Saccharomyces cerevisiae yeast strains due to saccharide/lectin specific recognition...
  24. Hickman M, Rusche L. The Sir2-Sum1 complex represses transcription using both promoter-specific and long-range mechanisms to regulate cell identity and sexual cycle in the yeast Kluyveromyces lactis. PLoS Genet. 2009;5:e1000710 pubmed publisher
    ..We examined the functions of the non-duplicated Sir2 and its partners, Sir4 and Sum1, in the yeast Kluyveromyces lactis, a species that diverged from Saccharomyces prior to the duplication of Sir2 and Hst1...
  25. Tarrío N, García Leiro A, Cerdán M, González Siso M. The role of glutathione reductase in the interplay between oxidative stress response and turnover of cytosolic NADPH in Kluyveromyces lactis. FEMS Yeast Res. 2008;8:597-606 pubmed publisher
    The phosphoglucose isomerase mutant of the respiratory yeast Kluyveromyces lactis (rag2) is forced to metabolize glucose through the oxidative pentose phosphate pathway and shows an increased respiratory chain activity and reactive ..
  26. Zanni E, Farina F, Ricci A, Mancini P, Frank C, Palleschi C, et al. The Golgi alpha-1,6 mannosyltransferase KlOch1p of Kluyveromyces lactis is required for Ca2+/calmodulin-based signaling and for proper mitochondrial functionality. BMC Cell Biol. 2009;10:86 pubmed publisher
    ..Here we report that, in Kluyveromyces lactis, this glycosyltransferase is also required to maintain functional mitochondria and calcium homeostasis...
  27. Nonklang S, Abdel Banat B, Cha aim K, Moonjai N, Hoshida H, Limtong S, et al. High-temperature ethanol fermentation and transformation with linear DNA in the thermotolerant yeast Kluyveromyces marxianus DMKU3-1042. Appl Environ Microbiol. 2008;74:7514-21 pubmed publisher
    We demonstrate herein the ability of Kluyveromyces marxianus to be an efficient ethanol producer and host for expressing heterologous proteins as an alternative to Saccharomyces cerevisiae. Growth and ethanol production by strains of K...
  28. Madinger C, Sharma S, Anton B, Fields L, Cushing M, Canovas J, et al. The effect of carbon source on the secretome of Kluyveromyces lactis. Proteomics. 2009;9:4744-54 pubmed publisher
    ..following bioreactor propagation of a wild-type industrial strain to identify proteins naturally secreted by Kluyveromyces lactis cells. Here, we report changes detected in the K...
  29. Weinberg D, Nakanishi K, Patel D, Bartel D. The inside-out mechanism of Dicers from budding yeasts. Cell. 2011;146:262-76 pubmed publisher
    ..Here, we describe the structure and activity of a catalytically active fragment of Kluyveromyces polysporus Dcr1, which represents the noncanonical Dicers found in budding yeasts...
  30. Read J, Colussi P, Ganatra M, Taron C. Acetamide selection of Kluyveromyces lactis cells transformed with an integrative vector leads to high-frequency formation of multicopy strains. Appl Environ Microbiol. 2007;73:5088-96 pubmed
    The yeast Kluyveromyces lactis has been extensively used as a host for heterologous protein expression. A necessary step in the construction of a stable expression strain is the introduction of an integrative expression vector into K...
  31. Navarro Olmos R, Kawasaki L, Dominguez Ramirez L, Ongay Larios L, Pérez Molina R, Coria R. The beta subunit of the heterotrimeric G protein triggers the Kluyveromyces lactis pheromone response pathway in the absence of the gamma subunit. Mol Biol Cell. 2010;21:489-98 pubmed publisher
    The Kluyveromyces lactis heterotrimeric G protein is a canonical Galphabetagamma complex; however, in contrast to Saccharomyces cerevisiae, where the Ggamma subunit is essential for mating, disruption of the KlGgamma gene yielded cells ..
  32. Micolonghi C, Wesolowski Louvel M, Bianchi M. The Rag4 glucose sensor is involved in the hypoxic induction of KlPDC1 gene expression in the yeast Kluyveromyces lactis. Eukaryot Cell. 2011;10:146-8 pubmed publisher
    b>Kluyveromyces lactis is a yeast which cannot grow under strict anaerobiosis. To date, no factors responsible for oxygen sensing and oxygen-dependent regulation of metabolism have been identified...
  33. Barsoum E, Rajaei N, Astr m S. RAS/cyclic AMP and transcription factor Msn2 regulate mating and mating-type switching in the yeast Kluyveromyces lactis. Eukaryot Cell. 2011;10:1545-52 pubmed publisher
    ..As sporulation requires a diploid DNA content, species with a haploid lifestyle, such as Kluyveromyces lactis, first induce mating in response to stress. In K...
  34. Arnold M, Durairaj V, Mundt E, Schulze K, Breunig K, Behrens S. Protective vaccination against infectious bursal disease virus with whole recombinant Kluyveromyces lactis yeast expressing the viral VP2 subunit. PLoS ONE. 2012;7:e42870 pubmed
    ..against infectious bursal disease (IBD) of poultry that were performed with complete yeast of the species Kluyveromyces lactis (K. lactis)...
  35. Camattari A, Bianchi M, Branduardi P, Porro D, Brambilla L. Induction by hypoxia of heterologous-protein production with the KlPDC1 promoter in yeasts. Appl Environ Microbiol. 2007;73:922-9 pubmed
    ..differing in size, origin, localization, and posttranslational modification, were successfully expressed in Kluyveromyces lactis under the control of the wild type or a modified promoter sequence, with a production ratio between 4 ..
  36. Carter S, Iyer S, Xu J, McEachern M, Aström S. The role of nonhomologous end-joining components in telomere metabolism in Kluyveromyces lactis. Genetics. 2007;175:1035-45 pubmed
    ..We explored these contradictory functions for NHEJ proteins by investigating their role in Kluyveromyces lactis telomere metabolism...
  37. Ponzoni C, Gasparetti C, Goretti M, Turchetti B, Pagnoni U, Cramarossa M, et al. Biotransformation of acyclic monoterpenoids by Debaryomyces sp., Kluyveromyces sp., and Pichia sp. strains of environmental origin. Chem Biodivers. 2008;5:471-83 pubmed publisher
    Sixty yeast strains, which belong to 32 species of the genera Debaryomyces, Kluyveromyces, and Pichia, and which were isolated from plant-, soil- or insect-associated habitats, were screened for their ability to biotransform the acyclic ..
  38. Rolland T, Neuvéglise C, Sacerdot C, Dujon B. Insertion of horizontally transferred genes within conserved syntenic regions of yeast genomes. PLoS ONE. 2009;4:e6515 pubmed publisher
    ..g. YwqG genes in Kluyveromyces thermotolerans and serine recombinase genes of the IS607 family in Saccharomyces kluyveri)...
  39. Fang Z, Wang G, Chen A, Li Y, Liu J, Li Y, et al. Gene responses to oxygen availability in Kluyveromyces lactis: an insight on the evolution of the oxygen-responding system in yeast. PLoS ONE. 2009;4:e7561 pubmed publisher
    ..HYP2/ANB1 and COX5A/COX5B are such gene pairs, and their unique orthologs in the 'non-WGD' Kluyveromyces lactis genome behaved like the aerobic versions of S. cerevisiae...
  40. Rocha S, Abrahão Neto J, Cerdán M, Gombert A, González Siso M. Heterologous expression of a thermophilic esterase in Kluyveromyces yeasts. Appl Microbiol Biotechnol. 2011;89:375-85 pubmed publisher
    In the present work, a thermophilic esterase from Thermus thermophilus HB27 was cloned into Kluyveromyces marxianus and into Kluyveromyces lactis using two different expression systems, yielding four recombinant strains. K...
  41. Singh R, Dhaliwal R, Puri M. Production of high fructose syrup from Asparagus inulin using immobilized exoinulinase from Kluyveromyces marxianus YS-1. J Ind Microbiol Biotechnol. 2007;34:649-55 pubmed
    Extracellular exoinulinase from Kluyveromyces marxianus YS-1, which hydrolyzes inulin into fructose, was immobilized on Duolite A568 after partial purification by ethanol precipitation and gel exclusion chromatography on Sephadex G-100...
  42. Rodicio R, Buchwald U, Schmitz H, Heinisch J. Dissecting sensor functions in cell wall integrity signaling in Kluyveromyces lactis. Fungal Genet Biol. 2008;45:422-35 pubmed
    KlWSC1, KlWSC2/3 and KlMID2, which encode putative plasma membrane sensors for cell wall integrity signaling in Kluyveromyces lactis, were cloned and characterized...
  43. Bechard L, Butuner B, Peterson G, McRae W, Topcu Z, McEachern M. Mutant telomeric repeats in yeast can disrupt the negative regulation of recombination-mediated telomere maintenance and create an alternative lengthening of telomeres-like phenotype. Mol Cell Biol. 2009;29:626-39 pubmed publisher
    ..In Kluyveromyces lactis mutants lacking telomerase, recombinational telomere elongation (RTE) is induced at short telomeres but ..
  44. Torres A, Maceiras M, Belmonte E, Naveira L, Calvo M, Cerdán M. KlRox1p contributes to yeast resistance to metals and is necessary for KlYCF1 expression in the presence of cadmium. Gene. 2012;497:27-37 pubmed publisher
    We have characterized the KlROX1 gene from Kluyveromyces lactis and verified that it does not regulate the hypoxic response in this yeast, oppositely to the Saccharomyces cerevisiae homologue ScROX1...
  45. Jeong H, Lee D, Kim S, Kim H, Lee K, Song J, et al. Genome sequence of the thermotolerant yeast Kluyveromyces marxianus var. marxianus KCTC 17555. Eukaryot Cell. 2012;11:1584-5 pubmed publisher
    b>Kluyveromyces marxianus is a thermotolerant yeast that has been explored for potential use in biotechnological applications, such as production of biofuels, single-cell proteins, enzymes, and other heterologous proteins...
  46. Oliveira G, Tahara E, Gombert A, Barros M, Kowaltowski A. Increased aerobic metabolism is essential for the beneficial effects of caloric restriction on yeast life span. J Bioenerg Biomembr. 2008;40:381-8 pubmed publisher
    ..Furthermore, in Kluyveromyces lactis, a Crabtree-negative yeast, glucose limitation did not promote an enhancement of respiratory capacity ..
  47. Koleva D, Petrova V, Kujumdzieva A. Comparison of enzymatic antioxidant defence systems in different metabolic types of yeasts. Can J Microbiol. 2008;54:957-63 pubmed publisher
    The enzymatic defence system in the 2 yeasts Kluyveromyces marxianus and Rhodotorula glutinis, differing in their mode of oxygen uptake and energy generation, was characterized and compared with the well-studied facultatively ..
  48. Cho U, Harrison S. Recognition of the centromere-specific histone Cse4 by the chaperone Scm3. Proc Natl Acad Sci U S A. 2011;108:9367-71 pubmed publisher
    ..the histone-fold domains of Cse4, and H4, all prepared as recombinant proteins derived from the budding yeast Kluyveromyces lactis...
  49. Raimondi S, Zanni E, Talora C, Rossi M, Palleschi C, Uccelletti D. SOD1, a new Kluyveromyces lactis helper gene for heterologous protein secretion. Appl Environ Microbiol. 2008;74:7130-7 pubmed publisher
    ..We found that overexpression of the Kluyveromyces lactis SOD1 (KlSOD1) gene was able to increase the production of two different heterologous proteins, human ..
  50. Blanco M, Núñez L, Tarrío N, Canto E, Becerra M, González Siso M, et al. An approach to the hypoxic and oxidative stress responses in Kluyveromyces lactis by analysis of mRNA levels. FEMS Yeast Res. 2007;7:702-14 pubmed
    Genome duplication, after the divergence of Saccharomyces cerevisiae from Kluyveromyces lactis along evolution, has been proposed as a mechanism of yeast evolution from strict aerobics, such as Candida albicans, to facultatives/..
  51. Bucciarelli T, Saliola M, Brisdelli F, Bozzi A, Falcone C, Di Ilio C, et al. Oxidation of Cys278 of ADH I isozyme from Kluyveromyces lactis by naturally occurring disulfides causes its reversible inactivation. Biochim Biophys Acta. 2009;1794:563-8 pubmed publisher
    The inactivation of the homotetrameric cytosolic alcohol dehydrogenase I from Kluyveromyces lactis (KlADH I) by naturally occurring disulfides, oxidized glutathione, cystine and cystamine, was studied...
  52. Raimondi S, Uccelletti D, Amaretti A, Leonardi A, Palleschi C, Rossi M. Secretion of Kluyveromyces lactis Cu/Zn SOD: strategies for enhanced production. Appl Microbiol Biotechnol. 2010;86:871-8 pubmed publisher
    The Kluyveromyces lactis Cu/Zn SOD gene (SOD1) was fused with the toxin K1 signal sequence to obtain extracellular production of superoxide dismutase. Kluyveromyces marxianus L3 and K...
  53. Ganatra M, Vainauskas S, Hong J, Taylor T, Denson J, Esposito D, et al. A set of aspartyl protease-deficient strains for improved expression of heterologous proteins in Kluyveromyces lactis. FEMS Yeast Res. 2011;11:168-78 pubmed publisher
    Secretion of recombinant proteins is a common strategy for heterologous protein expression using the yeast Kluyveromyces lactis...
  54. Hughes S, Bang S, Cox E, Schoepke A, Ochwat K, Pinkelman R, et al. Automated UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 and selection for microaerophilic growth and ethanol production at elevated temperature on biomass sugars. J Lab Autom. 2013;18:276-90 pubmed publisher
    The yeast Kluyveromyces marxianus is a potential microbial catalyst for fuel ethanol production from a wide range of biomass substrates. To improve its growth and ethanol yield at elevated temperature under microaerophilic conditions, K...
  55. Cash D, Cohen Zontag O, Kim N, Shefer K, Brown Y, Ulyanov N, et al. Pyrimidine motif triple helix in the Kluyveromyces lactis telomerase RNA pseudoknot is essential for function in vivo. Proc Natl Acad Sci U S A. 2013;110:10970-5 pubmed publisher
    ..The solution NMR structure of the Kluyveromyces lactis pseudoknot, presented here, reveals that it contains a long pyrimidine motif triple helix with ..
  56. Marchi E, Lodi T, Donnini C. KNQ1, a Kluyveromyces lactis gene encoding a transmembrane protein, may be involved in iron homeostasis. FEMS Yeast Res. 2007;7:715-21 pubmed
    ..of the experiments described in this article was to identify, in the biotechnologically important yeast Kluyveromyces lactis, gene(s) that are potentially involved in oxidative protein folding within the endoplasmic reticulum (..
  57. Ongay Larios L, Navarro Olmos R, Kawasaki L, Velázquez Zavala N, Sánchez Paredes E, Torres Quiroz F, et al. Kluyveromyces lactis sexual pheromones. Gene structures and cellular responses to alpha-factor. FEMS Yeast Res. 2007;7:740-7 pubmed
    The Kluyveromyces lactis genes for sexual pheromones have been analyzed. The alpha-factor gene encodes a predicted polypeptide of 187 amino acid residues containing four tridecapeptide repeats (WSWITLRPGQPIF)...
  58. Garc a Leiro A, Cerd n M, Gonz lez Siso M. A functional analysis of Kluyveromyces lactis glutathione reductase. Yeast. 2010;27:431-41 pubmed publisher
    Glutathione reductase (GLR) null mutants of the yeast Kluyveromyces lactis, a model eukaryotic respiratory cell, were created and phenotypically analysed...
  59. Liu H, Liu J, Tan B, Zhou F, Qin Y, Yang R. Covalent immobilization of Kluyveromyces fragilis ?-galactosidase on magnetic nanosized epoxy support for synthesis of galacto-oligosaccharide. Bioprocess Biosyst Eng. 2012;35:1287-95 pubmed publisher
    ..The magnetic poly(GMA-EDGMA-HEMA) nanobeads were used as support for covalent immobilization of Kluyveromyces fragilis ?-galactosidase, the maximum amount enzyme attached onto the support was 145...
  60. Zhang B, Li L, Zhang J, Gao X, Wang D, Hong J. Improving ethanol and xylitol fermentation at elevated temperature through substitution of xylose reductase in Kluyveromyces marxianus. J Ind Microbiol Biotechnol. 2013;40:305-16 pubmed publisher
    Thermo-tolerant yeast Kluyveromyces marxianus is able to utilize a wide range of substrates, including xylose; however, the xylose fermentation ability is weak because of the redox imbalance under oxygen-limited conditions...
  61. Lachance M. Current status of Kluyveromyces systematics. FEMS Yeast Res. 2007;7:642-5 pubmed
    A brief outline of the current taxonomic status of the genus Kluyveromyces is presented. Noteworthy are the transfer of several former Kluyveromyces species to other genera, the retention of the name Kluyveromyces for K. lactis, K...
  62. Coulon J, Matoub L, Dossot M, Marchand S, Bartosz G, Leroy P. Potential relationship between glutathione metabolism and flocculation in the yeast Kluyveromyces lactis. FEMS Yeast Res. 2007;7:93-101 pubmed
    ..Two yeast strains, a flocculent (Kluyveromyces lactis 5c) and a nonflocculent (Kluyveromyces lactis 5a) strain, were used...
  63. Cesare A, Groff Vindman C, Compton S, McEachern M, Griffith J. Telomere loops and homologous recombination-dependent telomeric circles in a Kluyveromyces lactis telomere mutant strain. Mol Cell Biol. 2008;28:20-9 pubmed
    The Kluyveromyces lactis ter1-16T strain contains mutant telomeres that are poorly bound by Rap1, resulting in a telomere-uncapping phenotype and significant elongation of the telomeric DNA...
  64. Bechard L, Jamieson N, McEachern M. Recombination can cause telomere elongations as well as truncations deep within telomeres in wild-type Kluyveromyces lactis cells. Eukaryot Cell. 2011;10:226-36 pubmed publisher
    In this study, we examined the role of recombination at the telomeres of the yeast Kluyveromyces lactis...
  65. Núñez L, Rodríguez Torres A, Cerdán M. Regulatory elements in the KlHEM1 promoter. Biochim Biophys Acta. 2008;1779:128-33 pubmed
    In Kluyveromyces lactis the gene encoding 5-aminolevulinate synthase, KlHEM1, is regulated at the transcriptional level by carbon source and oxygen availability...
  66. Swaim C, Anton B, Sharma S, Taron C, Benner J. Physical and computational analysis of the yeast Kluyveromyces lactis secreted proteome. Proteomics. 2008;8:2714-23 pubmed publisher
    Secretion of proteins is the most common approach to protein expression in Kluyveromyces lactis...
  67. Groeneveld P, Stouthamer A, Westerhoff H. Super life--how and why 'cell selection' leads to the fastest-growing eukaryote. FEBS J. 2009;276:254-70 pubmed publisher
    ..By applying pH-auxostat cultivation to the already fast-growing yeast Kluyveromyces marxianus for a sufficiently long time, we selected a strain with a 30% increased growth rate; its cell-cycle ..
  68. Yanase S, Hasunuma T, Yamada R, Tanaka T, Ogino C, Fukuda H, et al. Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes. Appl Microbiol Biotechnol. 2010;88:381-8 pubmed publisher
    ..Therefore, in the present study, a thermotolerant yeast, Kluyveromyces marxianus, which has high growth and fermentation at elevated temperatures, was used as a producer of ethanol ..
  69. Rodrussamee N, Lertwattanasakul N, Hirata K, Suprayogi -, Limtong S, Kosaka T, et al. Growth and ethanol fermentation ability on hexose and pentose sugars and glucose effect under various conditions in thermotolerant yeast Kluyveromyces marxianus. Appl Microbiol Biotechnol. 2011;90:1573-86 pubmed publisher
    Ethanol fermentation ability of the thermotolerant yeast Kluyveromyces marxianus, which is able to utilize various sugars including glucose, mannose, galactose, xylose, and arabinose, was examined under shaking and static conditions at ..
  70. Lulu L, Ling Z, Dongmei W, Xiaolian G, Hisanori T, Hidehiko K, et al. Identification of a xylitol dehydrogenase gene from Kluyveromyces marxianus NBRC1777. Mol Biotechnol. 2013;53:159-69 pubmed publisher
    ..A xylitol dehydrogenase gene (XYL2) encoding a XDH was cloned from Kluyveromyces marxianus NBRC 1777, and the in vivo function was validated by disruption and complementation analysis...
  71. Lertwattanasakul N, Shigemoto E, Rodrussamee N, Limtong S, Thanonkeo P, Yamada M. The crucial role of alcohol dehydrogenase Adh3 in Kluyveromyces marxianus mitochondrial metabolism. Biosci Biotechnol Biochem. 2009;73:2720-6 pubmed
    The function of mitochondrial Adh3 in the thermotolerant yeast Kluyveromyces marxianus was investigated...
  72. Lamas Maceiras M, Freire Picos M, Torres A. Transcriptional repression by Kluyveromyces lactis Tup1 in Saccharomyces cerevisiae. J Ind Microbiol Biotechnol. 2011;38:79-84 pubmed publisher
    ..The KlTUP1 gene from the yeast Kluyveromyces lactis encodes for a protein with 83% similarity to Tup1 in S. cerevisiae...
  73. Arrizon J, Morel S, Gschaedler A, Monsan P. Purification and substrate specificities of a fructanase from Kluyveromyces marxianus isolated from the fermentation process of Mezcal. Bioresour Technol. 2011;102:3298-303 pubmed publisher
    A fructanase, produced by a Kluyveromyces marxianus strain isolated during the fermentation step of the elaboration process of "Mezcal de Guerrero" was purified and biochemically characterized...
  74. Colón M, Hernandez F, Lopez K, Quezada H, González J, López G, et al. Saccharomyces cerevisiae Bat1 and Bat2 aminotransferases have functionally diverged from the ancestral-like Kluyveromyces lactis orthologous enzyme. PLoS ONE. 2011;6:e16099 pubmed publisher
    ..lactis has been distributed in the two paralogous genes present in S. cerevisiae, and whether this conservation has impacted S. cerevisiae metabolism...
  75. Lane M, Burke N, Karreman R, Wolfe K, O Byrne C, Morrissey J. Physiological and metabolic diversity in the yeast Kluyveromyces marxianus. Antonie Van Leeuwenhoek. 2011;100:507-19 pubmed publisher
    b>Kluyveromyces marxianus is homothallic hemiascomycete yeast frequently isolated from dairy environments...
  76. López Alvarez A, Díaz Pérez A, Sosa Aguirre C, Macías Rodríguez L, Campos García J. Ethanol yield and volatile compound content in fermentation of agave must by Kluyveromyces marxianus UMPe-1 comparing with Saccharomyces cerevisiae baker's yeast used in tequila production. J Biosci Bioeng. 2012;113:614-8 pubmed publisher
    ..In this study, a yeast isolated from native residual agave must was identified as Kluyveromyces marxianus UMPe-1 by 26S rRNA sequencing...
  77. Goshima T, Tsuji M, Inoue H, Yano S, Hoshino T, Matsushika A. Bioethanol production from Lignocellulosic biomass by a novel Kluyveromyces marxianus strain. Biosci Biotechnol Biochem. 2013;77:1505-10 pubmed
    The yeast Kluyveromyces marxianus is considered as a potential alternative to Saccharomyces cerevisiae in producing ethanol as a biofuel. In this study, we investigated the ethanol fermentation properties of novel K...
  78. Kawasaki L, Castañeda Bueno M, Sánchez Paredes E, Velázquez Zavala N, Torres Quiroz F, Ongay Larios L, et al. Protein kinases involved in mating and osmotic stress in the yeast Kluyveromyces lactis. Eukaryot Cell. 2008;7:78-85 pubmed
    Systematic disruption of genes encoding kinases and mitogen-activated protein kinases (MAPKs) was performed in Kluyveromyces lactis haploid cells...
  79. Bao W, Guiard B, Fang Z, Donnini C, Gervais M, Passos F, et al. Oxygen-dependent transcriptional regulator Hap1p limits glucose uptake by repressing the expression of the major glucose transporter gene RAG1 in Kluyveromyces lactis. Eukaryot Cell. 2008;7:1895-905 pubmed publisher
    ..As opposed to the fermentative S. cerevisiae, Kluyveromyces lactis is an aerobic yeast species which shows different oxygen responses...
  80. Souciet J, Dujon B, Gaillardin C, Johnston M, Baret P, Cliften P, et al. Comparative genomics of protoploid Saccharomycetaceae. Genome Res. 2009;19:1696-709 pubmed publisher
    ..We determined the complete genome sequences of three of these species: Kluyveromyces (Lachancea) thermotolerans and Saccharomyces (Lachancea) kluyveri (two members of the newly described ..
  81. Liu B, Gong X, Chang S, Yang Y, Song M, Duan D, et al. Disruption of the OCH1 and MNN1 genes decrease N-glycosylation on glycoprotein expressed in Kluyveromyces lactis. J Biotechnol. 2009;143:95-102 pubmed publisher
    Glycoproteins secreted by the yeast Kluyveromyces lactis are usually modified by the addition at asparagines-linked glycosylation sites of heterogeneous mannan residues. The secreted glycoproteins in K...
  82. Backhaus K, Heilmann C, Sorgo A, Purschke G, de Koster C, Klis F, et al. A systematic study of the cell wall composition of Kluyveromyces lactis. Yeast. 2010;27:647-60 pubmed publisher
    ..We here present a first systematic analysis of the cell wall composition of the milk yeast, Kluyveromyces lactis. Electron microscopic analyses revealed that exponentially growing cells of K...
  83. Galopier A, Hermann Le Denmat S. Mitochondria of the yeasts Saccharomyces cerevisiae and Kluyveromyces lactis contain nuclear rDNA-encoded proteins. PLoS ONE. 2011;6:e16325 pubmed publisher
    ..cerevisiae (Sc) and Kluyveromyces lactis (Kl) species and found that both proteins localize to mitochondria...
  84. Chang J, Xiang S, Xiang K, Manley J, Tong L. Structural and biochemical studies of the 5'?3' exoribonuclease Xrn1. Nat Struct Mol Biol. 2011;18:270-6 pubmed publisher
    ..Here we report the crystal structures of Kluyveromyces lactis Xrn1 (residues 1-1,245, E178Q mutant), alone and in complex with a Mn(2+) ion in the active site...
  85. Moreno A, Ibarra D, Fernandez J, Ballesteros M. Different laccase detoxification strategies for ethanol production from lignocellulosic biomass by the thermotolerant yeast Kluyveromyces marxianus CECT 10875. Bioresour Technol. 2012;106:101-9 pubmed publisher
    ..efficiency was analyzed on enzymatic hydrolysis and fermentation levels by the thermotolerant yeast Kluyveromyces marxianus. Laccases reduced phenolic compounds without affecting weak acids and furan derivates...
  86. Verma M, Barrow C, Kennedy J, Puri M. Immobilization of ?-d-galactosidase from Kluyveromyces lactis on functionalized silicon dioxide nanoparticles: characterization and lactose hydrolysis. Int J Biol Macromol. 2012;50:432-7 pubmed publisher
    D-Galactosidase (BGAL) from Kluyveromyces lactis was covalently immobilized to functionalized silicon dioxide nanoparticles (10-20 nm)...
  87. Wang R, Li L, Zhang B, Gao X, Wang D, Hong J. Improved xylose fermentation of Kluyveromyces marxianus at elevated temperature through construction of a xylose isomerase pathway. J Ind Microbiol Biotechnol. 2013;40:841-54 pubmed publisher
    To improve the xylose fermentation ability of Kluyveromyces marxianus, a xylose assimilation pathway through xylose isomerase was constructed...
  88. Penna M, Etcheverry M. Impact on growth and aflatoxin B1 accumulation by Kluyveromyces isolates at different water activity conditions. Mycopathologia. 2006;162:347-53 pubmed
    ..mycelial growth and aflatoxin B(1) accumulation when interacting Aspergillus aflatoxigenic strains with Kluyveromyces isolates and the effect of water activity on this relationship...
  89. Fonseca G, Gombert A, Heinzle E, Wittmann C. Physiology of the yeast Kluyveromyces marxianus during batch and chemostat cultures with glucose as the sole carbon source. FEMS Yeast Res. 2007;7:422-35 pubmed
    Growth, substrate consumption, metabolite formation, biomass composition and respiratory parameters of Kluyveromyces marxianus ATCC 26548 were determined during aerobic batch and chemostat cultivations, using mineral medium with glucose ..
  90. Faraco V, Ercole C, Festa G, Giardina P, Piscitelli A, Sannia G. Heterologous expression of heterodimeric laccase from Pleurotus ostreatus in Kluyveromyces lactis. Appl Microbiol Biotechnol. 2008;77:1329-35 pubmed
    ..structure of the POXA3 complex, a new system for recombinant expression of heterodimer proteins in the yeast Kluyveromyces lactis has been set up...
  91. Suryawati L, Wilkins M, Bellmer D, Huhnke R, Maness N, Banat I. Simultaneous saccharification and fermentation of Kanlow switchgrass pretreated by hydrothermolysis using Kluyveromyces marxianus IMB4. Biotechnol Bioeng. 2008;101:894-902 pubmed publisher
    A thermotolerant yeast strain named Kluyveromyces marxianus IMB4 was used in a simultaneous saccharification and fermentation (SSF) process using Kanlow switchgrass as a feedstock...
  92. Nonklang S, Ano A, Abdel Banat B, Saito Y, Hoshida H, Akada R. Construction of flocculent Kluyveromyces marxianus strains suitable for high-temperature ethanol fermentation. Biosci Biotechnol Biochem. 2009;73:1090-5 pubmed
    ..However, native yeasts are usually non-flocculating, including Kluyveromyces marxianus, which exhibits a potent high-temperature ethanol fermentation ability...