fasciola hepatica

Summary

Summary: A species of helminth commonly called the sheep liver fluke. It occurs in the biliary passages, liver, and gallbladder during various stages of development. Snails and aquatic vegetation are the intermediate hosts. Occasionally seen in man, it is most common in sheep and cattle.

Top Publications

  1. Lopez M, White A, Cabada M. Burden of Fasciola hepatica Infection among children from Paucartambo in Cusco, Peru. Am J Trop Med Hyg. 2012;86:481-5 pubmed publisher
    ..Parasite eggs and/or larvae were identified in 46.2% of subjects, including Fasciola hepatica in 10.3% of subjects. Fascioliasis was independently associated with anemia (adjusted odds ratio = 3.01 [1...
  2. Walker S, Johnston C, Hoey E, Fairweather I, Borgsteede F, Gaasenbeek C, et al. Population dynamics of the liver fluke, Fasciola hepatica: the effect of time and spatial separation on the genetic diversity of fluke populations in the Netherlands. Parasitology. 2011;138:215-23 pubmed publisher
    An evaluation of the genetic diversity within Fasciola hepatica (liver fluke) may provide an insight into its potential to respond to environmental changes, such as anthelmintic use or climate change...
  3. Salimi Bejestani M, Daniel R, Cripps P, Felstead S, Williams D. Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Fasciola hepatica in milk. Vet Parasitol. 2007;149:290-3 pubmed
    Antibodies against Fasciola hepatica were detected in serum and individual milk samples of dairy cattle using an ELISA...
  4. Buffoni L, Martinez Moreno F, Zafra R, Mendes R, Pérez Ecija A, Sekiya M, et al. Humoral immune response in goats immunised with cathepsin L1, peroxiredoxin and Sm14 antigen and experimentally challenged with Fasciola hepatica. Vet Parasitol. 2012;185:315-21 pubmed publisher
    ..immune response was analysed in goats immunised with FhCL1, FhPrx, Sm14, and experimentally challenged with Fasciola hepatica. All immunised animals developed significant levels of anti-fluke specific antibodies and those immunised ..
  5. Pointier J, Coustau C, Rondelaud D, Theron A. Pseudosuccinea columella (Say 1817) (Gastropoda, Lymnaeidae), snail host of Fasciola hepatica: first record for France in the wild. Parasitol Res. 2007;101:1389-92 pubmed
    ..columella isolate obtained in the laboratory was used for parasitological tests with a French Fasciola hepatica sample. Experimental infections showed that 100% of snails (n=26) developed infection when exposed to F...
  6. Khan M, Sajid M, Khan M, Iqbal Z, Arshad M, Hussain A. Point prevalence of bovine fascioliasis and the influence of chemotherapy on the milk yield in a lactating bovine population from the district of Toba Tek Singh, Pakistan. J Helminthol. 2011;85:334-8 pubmed publisher
    ..The economic value of reduced production of infected animals was estimated as US$0.33 and 0.32 per animal per day for cattle and buffaloes, respectively...
  7. Robinson M, Dalton J, Donnelly S. Helminth pathogen cathepsin proteases: it's a family affair. Trends Biochem Sci. 2008;33:601-8 pubmed publisher
    ..The liver fluke Fasciola hepatica, an emerging human pathogen, expresses the largest cathepsin L cysteine protease family yet described...
  8. Obchoei S, Weakley S, Wongkham S, Wongkham C, Sawanyawisuth K, Yao Q, et al. Cyclophilin A enhances cell proliferation and tumor growth of liver fluke-associated cholangiocarcinoma. Mol Cancer. 2011;10:102 pubmed publisher
    ..The ERK1/2 pathway may be involved in the CypA-mediated CCA cell proliferation. Thus, CypA may represent an important new therapeutic target for liver fluke-associated CCA. ..
  9. McGonigle L, Mousley A, Marks N, Brennan G, Dalton J, Spithill T, et al. The silencing of cysteine proteases in Fasciola hepatica newly excysted juveniles using RNA interference reduces gut penetration. Int J Parasitol. 2008;38:149-55 pubmed
    ..Here, we show the successful silencing of the cysteine proteases cathepsin B and L in the infective stage of Fasciola hepatica newly excysted juveniles (NEJs)...

More Information

Publications92

  1. Robinson M, Menon R, Donnelly S, Dalton J, Ranganathan S. An integrated transcriptomics and proteomics analysis of the secretome of the helminth pathogen Fasciola hepatica: proteins associated with invasion and infection of the mammalian host. Mol Cell Proteomics. 2009;8:1891-907 pubmed publisher
    To infect their mammalian hosts, Fasciola hepatica larvae must penetrate and traverse the intestinal wall of the duodenum, move through the peritoneum, and penetrate the liver...
  2. Salimi Bejestani M, Cripps P, Williams D. Evaluation of an ELISA to assess the intensity of Fasciola hepatica infection in cattle. Vet Rec. 2008;162:109-11 pubmed
    ..of 98 per cent and specificity of 96 per cent was evaluated as a means of assessing the intensity of Fasciola hepatica infection in cattle...
  3. Lowther J, Robinson M, Donnelly S, Xu W, Stack C, Matthews J, et al. The importance of pH in regulating the function of the Fasciola hepatica cathepsin L1 cysteine protease. PLoS Negl Trop Dis. 2009;3:e369 pubmed publisher
    The helminth parasite Fasciola hepatica secretes cathepsin L cysteine proteases to invade its host, migrate through tissues and digest haemoglobin, its main source of amino acids...
  4. Marcilla A, De la Rubia J, Sotillo J, Bernal D, Carmona C, Villavicencio Z, et al. Leucine aminopeptidase is an immunodominant antigen of Fasciola hepatica excretory and secretory products in human infections. Clin Vaccine Immunol. 2008;15:95-100 pubmed
    The liver fluke Fasciola hepatica parasitizes humans and ruminant livestock worldwide, and it is now being considered a reemerging zoonotic disease, especially in areas in which it is endemic, such as South America...
  5. Villa Mancera A, Méndez Mendoza M. Protection and antibody isotype responses against Fasciola hepatica with specific antibody to pIII-displayed peptide mimotopes of cathepsin L1 in sheep. Vet J. 2012;194:108-12 pubmed publisher
    ..immune response were assessed in sheep immunized with peptide mimotopes of cathepsin L1 and challenged with Fasciola hepatica metacercariae...
  6. Norbury L, Hung A, Beckham S, Pike R, Spithill T, Craik C, et al. Analysis of Fasciola cathepsin L5 by S2 subsite substitutions and determination of the P1-P4 specificity reveals an unusual preference. Biochimie. 2012;94:1119-27 pubmed publisher
    ..Finally, the P(1)-P(4) specificity of Fasciola cathepsin L5 was determined and, unexpectedly, aspartic acid was shown to be well accepted at P(2,) which is unique amongst Fasciola cathepsins examined to date. ..
  7. Miller C, Smith N, Ikin R, Boulter N, Dalton J, Donnelly S. Immunological interactions between 2 common pathogens, Th1-inducing protozoan Toxoplasma gondii and the Th2-inducing helminth Fasciola hepatica. PLoS ONE. 2009;4:e5692 pubmed publisher
    ..In contrast, extracellular helminths such as Fasciola hepatica induce Th2 responses characterised by the production of IL-4, IL-5, IL-10 and IgG1 antibodies and ..
  8. Keiser J, Rinaldi L, Veneziano V, Mezzino L, Tanner M, Utzinger J, et al. Efficacy and safety of artemether against a natural Fasciola hepatica infection in sheep. Parasitol Res. 2008;103:517-22 pubmed publisher
    ..we assessed the efficacy and safety of artemether, a derivative of artemisinin, in sheep with a low natural Fasciola hepatica infection...
  9. Flynn R, Irwin J, Olivier M, Sekiya M, Dalton J, Mulcahy G. Alternative activation of ruminant macrophages by Fasciola hepatica. Vet Immunol Immunopathol. 2007;120:31-40 pubmed
    The helminth parasite, Fasciola hepatica, has a worldwide distribution and infects a wide variety of mammalian hosts, including ruminants and man...
  10. Rapsch C, Dahinden T, Heinzmann D, Torgerson P, Braun U, Deplazes P, et al. An interactive map to assess the potential spread of Lymnaea truncatula and the free-living stages of Fasciola hepatica in Switzerland. Vet Parasitol. 2008;154:242-9 pubmed publisher
    The intermediate host of Fasciola hepatica is Lymnaea truncatula in Switzerland. The snail and the free-living stages of the parasite require a moderate climate and moisture for survival, reproduction, and transmission...
  11. Guevara Flores A, Pardo J, Rendón J. Hysteresis in thioredoxin-glutathione reductase (TGR) from the adult stage of the liver fluke Fasciola hepatica. Parasitol Int. 2011;60:156-60 pubmed publisher
    Thioredoxin-glutathione reductase (TGR) was purified from the adult stage of the liver fluke Fasciola hepatica. At 38° C and pH 7.8, specific activity values were 10.2U mg(-1) and 64...
  12. Keiser J, Veneziano V, Rinaldi L, Mezzino L, Duthaler U, Cringoli G. Anthelmintic activity of artesunate against Fasciola hepatica in naturally infected sheep. Res Vet Sci. 2010;88:107-10 pubmed publisher
    ..Following up on promising results obtained with artemether in Fasciola hepatica infected sheep, we here report the efficacy and safety of artesunate in sheep with a natural F...
  13. Robinson M, Tort J, Lowther J, Donnelly S, Wong E, Xu W, et al. Proteomics and phylogenetic analysis of the cathepsin L protease family of the helminth pathogen Fasciola hepatica: expansion of a repertoire of virulence-associated factors. Mol Cell Proteomics. 2008;7:1111-23 pubmed publisher
    Cathepsin L proteases secreted by the helminth pathogen Fasciola hepatica have functions in parasite virulence including tissue invasion and suppression of host immune responses...
  14. Duthaler U, Smith T, Keiser J. In vivo and in vitro sensitivity of Fasciola hepatica to triclabendazole combined with artesunate, artemether, or OZ78. Antimicrob Agents Chemother. 2010;54:4596-604 pubmed publisher
    Triclabendazole resistance is continually documented from livestock, and hence new treatment strategies for Fasciola hepatica infections are needed...
  15. Gaudier J, Cabán Hernández K, Osuna A, Espino A. Biochemical characterization and differential expression of a 16.5-kilodalton tegument-associated antigen from the liver fluke Fasciola hepatica. Clin Vaccine Immunol. 2012;19:325-33 pubmed publisher
    A cDNA encoding a 16.5-kDa protein termed FhTP16.5 was identified by immunoscreening of a cDNA library from Fasciola hepatica adult flukes using pooled sera from rabbits infected with F. hepatica for 4 weeks...
  16. Junking M, Wongkham C, Sripa B, Sawanyawisuth K, Araki N, Wongkham S. Decreased expression of galectin-3 is associated with metastatic potential of liver fluke-associated cholangiocarcinoma. Eur J Cancer. 2008;44:619-26 pubmed publisher
    ..Regulation of galectin-3 expression may therefore be an alternative therapeutic approach to control metastasis of cholangiocarcinoma. ..
  17. Pérez Ecija R, Mendes R, Zafra R, Buffonni L, Martínez Moreno A, Perez J. Pathological and parasitological protection in goats immunised with recombinant cathepsin L1 and challenged with Fasciola hepatica. Vet J. 2010;185:351-3 pubmed publisher
    ..with recombinant cathepsin L1 (rCL1) plus Quil A (n=7) or Quil A alone (control; n=7) and challenged with Fasciola hepatica. There were no significant differences in fluke burdens (56+/-26 vs. 92+/-53), average fluke length (20...
  18. Dias A, Araújo J, Braga F, Araujo J, Puppin A, Fernandes F, et al. Biological control of Fasciola hepatica eggs with the Pochonia chlamydosporia fungus after passing through the cattle gastrointestinal tract. Parasitol Res. 2012;110:663-7 pubmed publisher
    Fasciolosis is a disease caused by Fasciola hepatica responsible for causing significant losses in livestock. This study aimed to evaluate the Pochonia chlamydosporia fungus (isolate VC1) on F...
  19. Walsh K, Brady M, Finlay C, Boon L, Mills K. Infection with a helminth parasite attenuates autoimmunity through TGF-beta-mediated suppression of Th17 and Th1 responses. J Immunol. 2009;183:1577-86 pubmed publisher
    ..Infection of mice with Fasciola hepatica resulted in recruitment of dendritic cells, macrophages, eosinophils, neutrophils, and CD4(+) T cells into ..
  20. Moxon J, LaCourse E, Wright H, Perally S, Prescott M, Gillard J, et al. Proteomic analysis of embryonic Fasciola hepatica: characterization and antigenic potential of a developmentally regulated heat shock protein. Vet Parasitol. 2010;169:62-75 pubmed publisher
    b>Fasciola hepatica is responsible for human disease and economic livestock loss on a global scale. We report the first post-genomic investigation of cellular proteins expressed by embryonic F...
  21. Corvo I, Cancela M, Cappetta M, Pi Denis N, Tort J, Roche L. The major cathepsin L secreted by the invasive juvenile Fasciola hepatica prefers proline in the S2 subsite and can cleave collagen. Mol Biochem Parasitol. 2009;167:41-7 pubmed publisher
    Secreted cysteine proteases are major players in host-parasite interactions; in Fasciola hepatica, a distinct group of cathepsins L was found to be predominantly expressed in the juvenile stages, but their enzymatic properties were ..
  22. Bennema S, Vercruysse J, Claerebout E, Schnieder T, Strube C, Ducheyne E, et al. The use of bulk-tank milk ELISAs to assess the spatial distribution of Fasciola hepatica, Ostertagia ostertagi and Dictyocaulus viviparus in dairy cattle in Flanders (Belgium). Vet Parasitol. 2009;165:51-7 pubmed publisher
    b>Fasciola hepatica, Ostertagia ostertagi and Dictyocaulus viviparus are helminth parasites with a wide distribution and an important economic impact in cattle in temperate climates. This paper describes the spatial distribution of F...
  23. Rinaldi G, Morales M, Cancela M, Castillo E, Brindley P, Tort J. Development of functional genomic tools in trematodes: RNA interference and luciferase reporter gene activity in Fasciola hepatica. PLoS Negl Trop Dis. 2008;2:e260 pubmed publisher
    ..the method in the human blood fluke Schistosoma mansoni and then confirmed its utility in the liver fluke Fasciola hepatica. We transformed newly excysted juveniles of F...
  24. Mezo M, González Warleta M, Castro Hermida J, Muiño L, Ubeira F. Association between anti-F. hepatica antibody levels in milk and production losses in dairy cows. Vet Parasitol. 2011;180:237-42 pubmed publisher
    This study was conducted to determine: (1) the associations between anti-Fasciola hepatica antibody levels in milk and some productive and reproductive parameters in dairy cattle, and (2) the threshold antibody level associated with loss ..
  25. Kuerpick B, Conraths F, Staubach C, Fröhlich A, Schnieder T, Strube C. Seroprevalence and GIS-supported risk factor analysis of Fasciola hepatica infections in dairy herds in Germany. Parasitology. 2013;140:1051-60 pubmed publisher
    ..9% of all German dairy herds. The BTM samples were analysed for antibodies against Fasciola hepatica using the excretory-secretory (ES) ELISA...
  26. Matanović K, Severin K, Martinkovic F, Simpraga M, Janicki Z, Barisic J. Hematological and biochemical changes in organically farmed sheep naturally infected with Fasciola hepatica. Parasitol Res. 2007;101:1657-61 pubmed
    ..Hematological and blood biochemical values in sheep spontaneously infected with liver fluke Fasciola hepatica were compared with equivalent values in 20 parasite-free sheep from organically farmed flock...
  27. Bennema S, Ducheyne E, Vercruysse J, Claerebout E, Hendrickx G, Charlier J. Relative importance of management, meteorological and environmental factors in the spatial distribution of Fasciola hepatica in dairy cattle in a temperate climate zone. Int J Parasitol. 2011;41:225-33 pubmed publisher
    b>Fasciola hepatica, a trematode parasite with a worldwide distribution, is the cause of important production losses in the dairy industry. Diagnosis is hampered by the fact that the infection is mostly subclinical...
  28. Kirchhofer C, Vargas M, Braissant O, Dong Y, Wang X, Vennerstrom J, et al. Activity of OZ78 analogues against Fasciola hepatica and Echinostoma caproni. Acta Trop. 2011;118:56-62 pubmed publisher
    ..Dose response relationships were determined against juvenile and adult Fasciola hepatica in rats and Echinostoma caproni in mice...
  29. Jedlina L, Kozak Ljunggren M, Wedrychowicz H. In vivo studies of the early, peritoneal, cellular and free radical response in rats infected with Fasciola hepatica by flow cytometric analysis. Exp Parasitol. 2011;128:291-7 pubmed publisher
    ..for T cells, cytotoxic NK cells (CNK) and free radicals production of rats at an early stage of infection by Fasciola hepatica. Male Sprague-Dawley rats were infected with 50 metacercariae of F...
  30. González L, Esteban J, Bargues M, Valero M, Ortiz P, Naquira C, et al. Hyperendemic human fascioliasis in Andean valleys: an altitudinal transect analysis in children of Cajamarca province, Peru. Acta Trop. 2011;120:119-29 pubmed publisher
    ..b>Fasciola hepatica was the most common helminth within a spectrum of 11-12 protozoan and 9-11 helminth species, 97...
  31. Espino A, Morales A, Delgado B, Rivera F, Figueroa O, Suarez E. Partial immunity to Fasciola hepatica in mice after vaccination with FhSAP2 delivered as recombinant protein or DNA construct. Ethn Dis. 2010;20:S1-17-23 pubmed
    We recently reported the biochemical characterization of a novel Fasciola hepatica recombinant antigen termed FhSAP2, which has previously shown to elicit protection to F. hepatica infection in rabbits...
  32. Kozak M, Wedrychowicz H. The performance of a PCR assay for field studies on the prevalence of Fasciola hepatica infection in Galba truncatula intermediate host snails. Vet Parasitol. 2010;168:25-30 pubmed publisher
    ..The results on F. hepatica prevalence in snail hosts are the first data from Poland since the 1950s and the only such data based on molecular methods...
  33. Orr R, Kinkead R, Newman R, Anderson L, Hoey E, Trudgett A, et al. FhCaBP4: a Fasciola hepatica calcium-binding protein with EF-hand and dynein light chain domains. Parasitol Res. 2012;111:1707-13 pubmed
    ..A member of this family from the liver fluke, Fasciola hepatica-FhCaBP4-has been identified and characterised biochemically...
  34. Rokni M, Mirhendi H, Mizani A, Mohebali M, Sharbatkhori M, Kia E, et al. Identification and differentiation of Fasciola hepatica and Fasciola gigantica using a simple PCR-restriction enzyme method. Exp Parasitol. 2010;124:209-13 pubmed publisher
    Accurate morphological differentiation between the liver fluke species Fasciola hepatica and Fasciola gigantica is difficult...
  35. Relf V, Good B, McCarthy E, de Waal T. Evidence of Fasciola hepatica infection in Radix peregra and a mollusc of the family Succineidae in Ireland. Vet Parasitol. 2009;163:152-5 pubmed publisher
    ..genera Lymnaea, Pseudosuccinea, Galba and Stagnicola act as intermediate hosts of the common liver fluke, Fasciola hepatica. Galba truncatula is the only recorded intermediate host of F...
  36. Flynn R, Mulcahy G, Welsh M, Cassidy J, Corbett D, Milligan C, et al. Co-Infection of cattle with Fasciola hepatica and Mycobacterium bovis- immunological consequences. Transbound Emerg Dis. 2009;56:269-74 pubmed publisher
    b>Fasciola hepatica, the liver fluke, is a common parasite of cattle in much of the world. Previously, we have shown that cattle infected with F...
  37. Hammami H, Hamed N, Ayadi A. Epidemiological studies on Fasciola hepatica in Gafsa Oases (south west of Tunisia). Parasite. 2007;14:261-4 pubmed
    Epidemiological investigations on Fasciola hepatica fasciolasis were carried out from July 2004 to June 2005 in the Gafsa oases (Tunisia) after the detection of a human case...
  38. Falcón C, Carranza F, Aoki P, Motran C, Cervi L. Adoptive transfer of dendritic cells pulsed with Fasciola hepatica antigens and lipopolysaccharides confers protection against fasciolosis in mice. J Infect Dis. 2012;205:506-14 pubmed publisher
    ..Given that successful vaccination against Fasciola hepatica is mostly related to the induction of Th1 responses, we studied the potential of DCs loaded with F...
  39. Claridge J, Diggle P, McCann C, Mulcahy G, Flynn R, McNair J, et al. Fasciola hepatica is associated with the failure to detect bovine tuberculosis in dairy cattle. Nat Commun. 2012;3:853 pubmed publisher
    ..hepatica. This finding may in part explain the continuing spread of BTB and the failure of the current eradication programme in the United Kingdom...
  40. LaCourse E, Perally S, Morphew R, Moxon J, Prescott M, Dowling D, et al. The Sigma class glutathione transferase from the liver fluke Fasciola hepatica. PLoS Negl Trop Dis. 2012;6:e1666 pubmed publisher
    ..A new form of glutathione transferase (GST) family, Sigma class GST, closely related to a leading Schistosome vaccine candidate (Sm28), has previously been revealed by proteomics in the liver fluke but not functionally characterised...
  41. Sanabria R, Mouzet R, Courtioux B, Vignoles P, Rondelaud D, Dreyfuss G, et al. Intermediate snail hosts of French Fasciola hepatica: Lymnaea neotropica and Lymnaea viatrix are better hosts than local Galba truncatula. Parasitol Res. 2012;111:2011-6 pubmed publisher
    ..ventricosa with Argentinean and French isolates of Fasciola hepatica were carried out to determine the capacity of these snails to produce metacercariae and to verify if this ..
  42. Shu F, Lv R, Zhang Y, Duan G, Wu D, Li B, et al. Characterization of Fasciola samples by ITS of rDNA sequences revealed the existence of Fasciola hepatica and Fasciola gigantica in Yunnan Province, China. J Parasitol. 2012;98:889-90 pubmed publisher
    ..Using ITS sequences, 2 Fasciola species were revealed, namely Fasciola hepatica and Fasciola gigantica. This is the first demonstration of F...
  43. Donnelly S, Stack C, O Neill S, Sayed A, Williams D, Dalton J. Helminth 2-Cys peroxiredoxin drives Th2 responses through a mechanism involving alternatively activated macrophages. FASEB J. 2008;22:4022-32 pubmed publisher
    ..Using murine models for the helminth infections caused by Fasciola hepatica (Fh) and Schistosoma mansoni (Sm), we show that a secreted antioxidant, peroxiredoxin (Prx), induces ..
  44. Fairweather I. Reducing the future threat from (liver) fluke: realistic prospect or quixotic fantasy?. Vet Parasitol. 2011;180:133-43 pubmed publisher
    ..of increasing incidence and drug resistance) is the overall theme of the session on "Trematodes: Fasciola hepatica epidemiology and control" and of this review to introduce the session...
  45. Rondelaud D, Hourdin P, Vignoles P, Dreyfuss G, Cabaret J. The detection of snail host habitats in liver fluke infected farms by use of plant indicators. Vet Parasitol. 2011;181:166-73 pubmed publisher
    ..The positive sites may be detected using a two-step method including first categorization of hydrographical zones and then, within a zone, use of one or several indicator plants (frequently J. acutiflorus)...
  46. Buffoni L, Zafra R, Pérez Ecija A, Martinez Moreno F, Martínez Galisteo E, Moreno T, et al. Immune response of goats immunised with glutathione S-transferase and experimentally challenged with Fasciola hepatica. Parasitol Int. 2010;59:147-53 pubmed publisher
    Glutathione S-transferase (FhGST) purified from Fasciola hepatica adult worms was used to immunise goats against F...
  47. Marcos L, Bussalleu A, Terashima A, Espinoza J. Detection of antibodies against Fasciola hepatica in cirrhotic patients from Peru. J Helminthol. 2009;83:23-6 pubmed publisher
    The prevalence of Fasciola hepatica infection, in endemic countries, in patients with established cirrhosis is unknown...
  48. Young N, Hall R, Jex A, Cantacessi C, Gasser R. Elucidating the transcriptome of Fasciola hepatica - a key to fundamental and biotechnological discoveries for a neglected parasite. Biotechnol Adv. 2010;28:222-31 pubmed publisher
    ..Key representatives, such as Fasciola hepatica and F...
  49. Caron Y, Lasri S, Losson B. Fasciola hepatica: an assessment on the vectorial capacity of Radix labiata and R. balthica commonly found in Belgium. Vet Parasitol. 2007;149:95-103 pubmed
    ..two species and Galba truncatula (the major intermediate host in Europe) were experimentally infected with Fasciola hepatica. The resulting metacercariae were fed to rats and the infection was monitored using several techniques...
  50. Zafra R, Pérez Ecija R, Buffoni L, Moreno P, Bautista M, Martinez Moreno A, et al. Early and late peritoneal and hepatic changes in goats immunized with recombinant cathepsin L1 and infected with Fasciola hepatica. J Comp Pathol. 2013;148:373-84 pubmed publisher
    ..hepatica pro cathepsin L1 (rCL1) in Quil A and challenged with Fasciola hepatica. Despite finding no significant reduction in fluke burdens between the control and immunized group, at 15 ..
  51. Ubeira F, Muiño L, Valero M, Periago M, Perez Crespo I, Mezo M, et al. MM3-ELISA detection of Fasciola hepatica coproantigens in preserved human stool samples. Am J Trop Med Hyg. 2009;81:156-62 pubmed
    ..We concluded that the MM3-COPRO ELISA combined with the use of CoproGuard may be a very useful tool for the diagnosis of human fascioliasis...
  52. Zhao Q, Vargas M, Dong Y, Zhou L, Wang X, Sriraghavan K, et al. Structure-activity relationship of an ozonide carboxylic acid (OZ78) against Fasciola hepatica. J Med Chem. 2010;53:4223-33 pubmed publisher
    ..We found that relatively small structural changes to 1 resulted most commonly in loss of activity against Fasciola hepatica in vivo...
  53. McCann C, Baylis M, Williams D. The development of linear regression models using environmental variables to explain the spatial distribution of Fasciola hepatica infection in dairy herds in England and Wales. Int J Parasitol. 2010;40:1021-8 pubmed publisher
    Fasciolosis caused by Fasciola hepatica is a major cause of economic loss to the agricultural community worldwide as a result of morbidity and mortality in livestock...
  54. Magalhaes K, Jannotti Passos L, Caldeira R, Berne M, Muller G, Carvalho O, et al. Isolation and detection of Fasciola hepatica DNA in Lymnaea viatrix from formalin-fixed and paraffin-embedded tissues through multiplex-PCR. Vet Parasitol. 2008;152:333-8 pubmed publisher
    Detection of Fasciola hepatica infection in Lymnaea viatrix through analysis of histological cuts is based upon morphological characters of the parasite during the intra-mollusk phase of parasitism...
  55. Moxon J, Flynn R, Golden O, Hamilton J, Mulcahy G, Brophy P. Immune responses directed at egg proteins during experimental infection with the liver fluke Fasciola hepatica. Parasite Immunol. 2010;32:111-24 pubmed publisher
    b>Fasciola hepatica is responsible for human disease and economic livestock loss on a global scale. Unlike the well characterized schistosomes, only the adult and juvenile stages of F...
  56. Ozturhan H, Emekdas G, Sezgin O, Korkmaz M, Altintas E. Seroepidemiology of Fasciola Hepatica in Mersin province and surrounding towns and the role of family history of the Fascioliasis in the transmission of the parasite. Turk J Gastroenterol. 2009;20:198-203 pubmed
    Fascioliasis is an important zoonotic disease caused by Fasciola hepatica. This zoonosis may cause serious morbidity and a considerable financial burden...
  57. Hammami H, Ayadi A. Molluscicidal and antiparasitic activity of Solanum nigrum villosum against Galba truncatula infected or uninfected with Fasciola hepatica. J Helminthol. 2008;82:235-9 pubmed publisher
    ..have also been assessed on larval stages (rediae, intraredial germinal masses and cercariae) of the parasite Fasciola hepatica infecting G. truncatula naturally. The unripe fruit extract (LC50 = 41...
  58. Kaplan R. Fasciola hepatica: a review of the economic impact in cattle and considerations for control. Vet Ther. 2001;2:40-50 pubmed
    ..recommended control programs for liver flukes in cattle, the economic impact and the complex life cycle of Fasciola hepatica need to be appreciated...
  59. Fairweather I, McShane D, Shaw L, Ellison S, O Hagan N, York E, et al. Development of an egg hatch assay for the diagnosis of triclabendazole resistance in Fasciola hepatica: proof of concept. Vet Parasitol. 2012;183:249-59 pubmed publisher
    ..study was to develop an Egg Hatch Assay (EHA) test for the detection of triclabendazole (TCBZ) resistance in Fasciola hepatica. A number of fluke isolates were used, of differing sensitivity to TCBZ...
  60. Lotfollahzadeh S, Mohri M, Bahadori S, Dezfouly M, Tajik P. The relationship between normocytic, hypochromic anaemia and iron concentration together with hepatic enzyme activities in cattle infected with Fasciola hepatica. J Helminthol. 2008;82:85-8 pubmed publisher
    ..in infected and uninfected cattle to determine the type of anaemia and degree of hepatic damage caused by Fasciola hepatica. Blood samples from 86 infected and 30 uninfected cattle were taken at slaughter...
  61. Charlier J, Bennema S, Caron Y, Counotte M, Ducheyne E, Hendrickx G, et al. Towards assessing fine-scale indicators for the spatial transmission risk of Fasciola hepatica in cattle. Geospat Health. 2011;5:239-45 pubmed
    ..The habitats were visually characterised, investigated for the presence of the intermediate host snails of Fasciola hepatica (i.e...
  62. Mas Coma S, Valero M, Bargues M. Chapter 2. Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Adv Parasitol. 2009;69:41-146 pubmed publisher
    ..A complete understanding of molecular epidemiology is expected to help greatly in designing global actions and local interventions for control of fascioliasis...
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    Serum biomarkers associated with Fasciola hepatica infection of Corriedale sheep were analysed during the first 12 weeks of infection using surface-enhanced laser desorption ionisation time of flight mass spectrometry (SELDI-TOF MS)...
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    An M13 phage random 12-mers peptide library was used to screen cathepsin L mimotopes of Fasciola hepatica and to evaluate their immunogenicity in sheep...
  65. Martins I, de Avelar B, Pereira M, da Fonseca A. Application of a geographical information system approach for risk analysis of fascioliasis in southern Espírito Santo state, Brazil. Geospat Health. 2012;6:S87-93 pubmed
    ..These areas were found to be characterized by comparatively high temperature but relatively low slope, low precipitation and low elevation corresponding to periodically flooded grasslands or soils that promote water retention...
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    The population dynamics and prevalence of Fasciola hepatica in Galba truncatula were investigated on the Teagasc hill sheep farm in Country Mayo, Ireland from August 2006 until March 2008...
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    ..Each was used singly or in combination to vaccinate rats that were subsequently challenged with Fasciola hepatica metercercariae...
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    Protection against Fasciola hepatica in goats immunized with Peroxiredoxin (Prx) was assessed...
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    ..These differences may reflect a specialised role for FhCaM2 in the liver fluke...
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    An 18.2 kDa protein from the liver fluke, Fasciola hepatica has been identified and characterised...
  71. Zafra R, Pérez Ecija R, Buffoni L, Pacheco I, Martinez Moreno A, Lacourse E, et al. Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica. Res Vet Sci. 2013;94:602-9 pubmed publisher
    ..with recombinant glutathione transferase sigma class (rFhGST-S1) during early stages of infection with Fasciola hepatica. Group 1 (n=7) was unimmunized and uninfected; group 2 (n=10) was immunized with adjuvant Quil A and ..
  72. Farjallah S, Sanna D, Amor N, Ben Mehel B, Piras M, Merella P, et al. Genetic characterization of Fasciola hepatica from Tunisia and Algeria based on mitochondrial and nuclear DNA sequences. Parasitol Res. 2009;105:1617-21 pubmed publisher
    ..In the present study, samples identified morphologically as Fasciola hepatica from sheep and cattle from different geographical locations of Tunisia and Algeria were genetically ..
  73. Kr lov Hromadov I, Spakulov M, Hor ckov E, Turcekov L, Novobilsk A, Beck R, et al. Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica. J Parasitol. 2008;94:58-67 pubmed publisher
    ..The 18S and ITS sequences were compared with previously published sequences of the liver fluke Fasciola hepatica. Fixed interspecific genetic differences were determined that allow molecular differentiation of F...
  74. Cancela M, Acosta D, Rinaldi G, Silva E, Duran R, Roche L, et al. A distinctive repertoire of cathepsins is expressed by juvenile invasive Fasciola hepatica. Biochimie. 2008;90:1461-75 pubmed publisher
    ..In the trematode Fasciola hepatica, the initial step to successful infection of the mammalian host is the excystment of metacercariae and the ..
  75. Flynn R, Mulcahy G. The roles of IL-10 and TGF-beta in controlling IL-4 and IFN-gamma production during experimental Fasciola hepatica infection. Int J Parasitol. 2008;38:1673-80 pubmed publisher
    Hosts infected with Fasciola hepatica experience immunosuppression during the acute and chronic phases of the disease. This immunosuppression may allow parasite survival in the face of an ongoing immune response...
  76. Robinson M, Donnelly S, Hutchinson A, To J, Taylor N, Norton R, et al. A family of helminth molecules that modulate innate cell responses via molecular mimicry of host antimicrobial peptides. PLoS Pathog. 2011;7:e1002042 pubmed publisher
    ..The HDM secreted by Fasciola hepatica (FhHDM-1) adopts a predominantly ?-helical structure in solution. Processing of FhHDM-1 by F...
  77. Mezo M, González Warleta M, Castro Hermida J, Ubeira F. Evaluation of the flukicide treatment policy for dairy cattle in Galicia (NW Spain). Vet Parasitol. 2008;157:235-43 pubmed publisher
    b>Fasciola hepatica infection is an important cause of lost productivity in livestock worldwide...
  78. Devine C, Brennan G, Lanusse C, Alvarez L, Trudgett A, Hoey E, et al. Effect of the metabolic inhibitor, methimazole on the drug susceptibility of a triclabendazole-resistant isolate of Fasciola hepatica. Parasitology. 2009;136:183-92 pubmed publisher
    ..Results of this study support the concept of altered drug metabolism in TCBZ-resistant flukes and this process may play a role in the development of drug resistance...
  79. Morphew R, Wright H, LaCourse E, Porter J, Barrett J, Woods D, et al. Towards delineating functions within the fasciola secreted cathepsin l protease family by integrating in vivo based sub-proteomics and phylogenetics. PLoS Negl Trop Dis. 2011;5:e937 pubmed publisher
    b>fasciola hepatica, along with Fasciola gigantica, is the causative agent of fasciolosis, a foodborne zoonotic disease affecting grazing animals and humans worldwide...
  80. Muiño L, Perteguer M, Garate T, Martínez Sernández V, Beltran A, Romaris F, et al. Molecular and immunological characterization of Fasciola antigens recognized by the MM3 monoclonal antibody. Mol Biochem Parasitol. 2011;179:80-90 pubmed publisher
    ..In addition, the ELISA inhibitions showed that the MM3 epitope is not recognized by rabbits, which explains the usefulness of these species for producing capture antibodies for use in MM3-ELISA assays...
  81. Hamed N, Hammami H, Khaled S, Rondelaud D, Ayadi A. Natural infection of Fasciola hepatica (Trematoda: Fasciolidae) in Bulinus truncatus (Gastropoda: Planorbidae) in northern Tunisia. J Helminthol. 2009;83:271-3 pubmed publisher
    ..a cattle-breeding farm located in the region of Sejnane (North Tunisia) to detect natural infections with Fasciola hepatica and determine seasonal variations of the prevalence throughout a year...
  82. Jaros S, Jaros D, Wesolowska A, Zygner W, Wedrychowicz H. Blocking Fasciola hepatica's energy metabolism - a pilot study of vaccine potential of a novel gene - phosphoglycerate kinase. Vet Parasitol. 2010;172:229-37 pubmed publisher
    b>Fasciola hepatica infections cause huge economic losses to livestock production and are a serious problem in human and veterinary medicine. The main difficulty in the control of infections is progressing drug resistance...
  83. Novobilský A, Engström A, Sollenberg S, Gustafsson K, Morrison D, Hoglund J. Transmission patterns of Fasciola hepatica to ruminants in Sweden. Vet Parasitol. 2014;203:276-86 pubmed publisher
    Transmission patterns of Fasciola hepatica were investigated on beef cattle (n=3) and sheep (n=3) farms in Sweden between 2011 and 2012...