fasciola

Summary

Summary: A genus of trematode liver flukes of the family Fasciolidae. Two species of this genus are F. hepatica and F. gigantica. The parasites are found in the liver and gallbladder and associated ducts in mammals and occasionally man. F. gigantica occurs rarely in man.

Top Publications

  1. Itagaki T, Kikawa M, Terasaki K, Shibahara T, Fukuda K. Molecular characterization of parthenogenic Fasciola sp. in Korea on the basis of DNA sequences of ribosomal ITS1 and mitochondrial NDI gene. J Vet Med Sci. 2005;67:1115-8 pubmed
    ..ITS1) and mitochondrial NADH dehydrogenase I (NDI) gene were analyzed to genetically characterize aspermic Fasciola forms in Korea...
  2. Le T, Nguyen K, Nguyen N, Doan H, Le X, Hoang C, et al. Development and evaluation of a single-step duplex PCR for simultaneous detection of Fasciola hepatica and Fasciola gigantica (family Fasciolidae, class Trematoda, phylum Platyhelminthes). J Clin Microbiol. 2012;50:2720-6 pubmed publisher
    ..multiplex PCR (here referred to as a duplex PCR) has been developed for simultaneous detection and diagnosis of Fasciola hepatica and F. gigantica...
  3. Smooker P, Kennedy N, Steeper K, Christopoulos H, Spithill T. Fasciola: kinetics and quality of humoral responses to fatty acid binding protein and cathepsin l following delivery as DNA vaccines in mice. Exp Parasitol. 2001;97:154-60 pubmed
    The humoral response to DNA vaccination of mice with two important Fasciola antigens has been investigated. Both F. gigantica fatty acid binding protein (FABP) and F...
  4. Mera y Sierra R, Artigas P, Cuervo P, Deis E, Sidoti L, Mas Coma S, et al. Fascioliasis transmission by Lymnaea neotropica confirmed by nuclear rDNA and mtDNA sequencing in Argentina. Vet Parasitol. 2009;166:73-9 pubmed publisher
    ..Redial larval stages found in a L. neotropica specimen were ascribed to Fasciola hepatica after analysis of the complete ITS-1 sequence. The finding of L...
  5. Dixit A, Yadav S, Sharma R. Experimental bubalian fasciolosis: kinetics of antibody response using 28 kDa Fasciola gigantica cysteine proteinase as antigen. Trop Anim Health Prod. 2004;36:49-54 pubmed
  6. Heneberg P. Phylogenetic data suggest the reclassification of Fasciola jacksoni (Digenea: Fasciolidae) as Fascioloides jacksoni comb. nov. Parasitol Res. 2013;112:1679-89 pubmed publisher
    b>Fasciola jacksoni (Cobbold, 1869) is a highly prevalent (18-62%) species colonizing the liver (less frequently the lungs, kidneys, pericardia, and intestines) of Elephas maximus indicus and Elephas maximus maximus in the Indomalayan ..
  7. Yabe J, Phiri I, Phiri A, Chembensofu M, Dorny P, Vercruysse J. Concurrent infections of Fasciola, Schistosoma and Amphistomum spp. in cattle from Kafue and Zambezi river basins of Zambia. J Helminthol. 2008;82:373-6 pubmed publisher
    This study investigated interactions among Fasciola gigantica, Schistosoma spp. and Amphistomum spp. concurrent natural infections in Zambian cattle, based on egg and worm counts. In the abattoir 315 cattle were screened for worms of F...
  8. Meemon K, Grams R, Vichasri Grams S, Hofmann A, Korge G, Viyanant V, et al. Molecular cloning and analysis of stage and tissue-specific expression of cathepsin B encoding genes from Fasciola gigantica. Mol Biochem Parasitol. 2004;136:1-10 pubmed
    The transcriptional products of Fasciola gigantica genes encoding cathepsin B proteases were cloned from adult, newly excysted juvenile (NEJ), and metacercarial stages. The obtained cDNAs were named FG cat-B1, FG cat-B2, and FG cat-B3...
  9. Zhang W, Moreau E, Huang W, Chauvin A. Comparison of humoral response in sheep to Fasciola hepatica and Fasciola gigantica experimental infection. Parasite. 2004;11:153-9 pubmed
    ..Further investigation must be done to study the mechanism of resistance between the sheep infected with F. hepatica or F. gigantica. ..

More Information

Publications91

  1. Lin R, Dong S, Nie K, Wang C, Song H, Li A, et al. Sequence analysis of the first internal transcribed spacer of rDNA supports the existence of the intermediate Fasciola between F. hepatica and F. gigantica in mainland China. Parasitol Res. 2007;101:813-7 pubmed
    ..conformation polymorphism (PCR-SSCP) approach combined with DNA sequencing was used to characterise samples of Fasciola spp. from different host species and geographical locations in mainland China...
  2. Barduagni P, Hassanein Y, Mohamed M, Wakeel A, Sayed M, Hallaj Z, et al. Use of triclabendazole for treatment of patients co-infected by Fasciola spp. and S. mansoni in Behera Governorate, Egypt. Parasitol Res. 2008;102:631-3 pubmed
    ..and Fasciola spp. It has been reported to be effective in vitro and in experimental animals against Schistosoma mansoni...
  3. Gunasekar K, Tewari A, Sreekumar C, Gupta S, Rao J. Elucidation of genetic variability among different isolates of Fasciola gigantica (giant liver fluke) using random-amplified polymorphic DNA polymerase chain reaction. Parasitol Res. 2008;103:1075-81 pubmed publisher
    The present communication focuses on molecular characterization of Fasciola gigantica isolates derived from cattle, buffalo, and goat using random-amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) analysis to elucidate ..
  4. Tantrawatpan C, Maleewong W, Wongkham C, Wongkham S, Intapan P, Nakashima K. Serodiagnosis of human fascioliasis by a cystatin capture enzyme-linked immunosorbent assay with recombinant Fasciola gigantica cathepsin L antigen. Am J Trop Med Hyg. 2005;72:82-6 pubmed
    Cathepsin L1, a cysteine protease secreted by the gastrodermis of juvenile and adult Fasciola gigantica, was expressed in Escherichia coli as a calmodulin binding peptide fusion protein with a molecular mass of approximately 35 kD...
  5. Chantree P, Phatsara M, Meemon K, Chaichanasak P, Changklungmoa N, Kueakhai P, et al. Vaccine potential of recombinant cathepsin B against Fasciola gigantica. Exp Parasitol. 2013;135:102-9 pubmed publisher
    In Fasciola gigantica, cathepsin Bs, especially cathepsin B2 and B3 are expressed in early juvenile stages, and are proposed to mediate the invasion of host tissues...
  6. Ashrafi K, Valero M, Panova M, Periago M, Massoud J, Mas Coma S. Phenotypic analysis of adults of Fasciola hepatica, Fasciola gigantica and intermediate forms from the endemic region of Gilan, Iran. Parasitol Int. 2006;55:249-60 pubmed
    Fascioliasis is an important human and animal disease caused by Fasciola hepatica and Fasciola gigantica...
  7. Lotfy W, El Morshedy H, Abou El Hoda M, El Tawila M, Omar E, Farag H. Identification of the Egyptian species of Fasciola. Vet Parasitol. 2002;103:323-32 pubmed
    Reports on the species of Fasciola present in the Nile Delta, Egypt, appear controversial. Some authors reported the presence of both Fasciola gigantica and Fasciola hepatica, others reported F. gigantica only and mentioned that F...
  8. Shu F, Lv R, Zhang Y, Duan G, Wu D, Li B, et al. Characterization of Fasciola samples by ITS of rDNA sequences revealed the existence of Fasciola hepatica and Fasciola gigantica in Yunnan Province, China. J Parasitol. 2012;98:889-90 pubmed publisher
    On mainland China, liver flukes of Fasciola spp. (Digenea: Fasciolidae) can cause serious acute and chronic morbidity in numerous species of mammals such as sheep, goats, cattle, and humans...
  9. Itagaki T, Ichinomiya M, Fukuda K, Fusyuku S, Carmona C. Hybridization experiments indicate incomplete reproductive isolating mechanism between Fasciola hepatica and Fasciola gigantica. Parasitology. 2011;138:1278-84 pubmed publisher
    Experiments on hybridization between Fasciola hepatica and Fasciola gigantica were carried out to clarify whether a reproductive isolating mechanism appears between the two Fasciola species...
  10. Haseeb A, El Shazly A, Arafa M, Morsy A. Evaluation of excretory/secretory Fasciola (Fhes) antigen in diagnosis of human fascioliasis. J Egypt Soc Parasitol. 2003;33:123-38 pubmed
    ..So, ELISA-Fhes antigen (Fasciola hepatica excretory/secretory) and IHAT were evaluated in the immunodiagnosis of parasitologically proven cases of ..
  11. Prasad P, Tandon V, Biswal D, Goswami L, Chatterjee A. Molecular identification of the Indian liver fluke, Fasciola (Trematoda: Fasciolidae) based on the ribosomal internal transcribed spacer regions. Parasitol Res. 2008;103:1247-55 pubmed publisher
    The species of liver flukes of the genus Fasciola (Platyhelminthes: Digenea: Fasciolidae) are obligate parasitic trematodes residing in the large biliary ducts of herbivorous mammals...
  12. Ali N. Development and evaluation of a dipstick assay in diagnosis of human fasciolosis. Parasitol Res. 2012;110:1649-54 pubmed publisher
    ..and its diagnostic efficacy was evaluated in comparison to enzyme linked immunosorbent assay (ELISA) using Fasciola gigantica excretory-secretory antigen...
  13. Raadsma H, Kingsford N, Suharyanta -, Spithill T, Piedrafita D. Host responses during experimental infection with Fasciola gigantica or Fasciola hepatica in Merino sheep I. Comparative immunological and plasma biochemical changes during early infection. Vet Parasitol. 2007;143:275-86 pubmed
    ..host-immune responses and parasite recovery data in Merino sheep during the first 10 weeks of infection with Fasciola gigantica and Fasciola hepatica...
  14. Zhang W, Rogniaux H, Huang W, Chauvin A, Moreau E. Analysis of thioredoxin peroxidase as a promising antigen for diagnosis of Fasciola gigantica infection: a preliminary study. Parasitol Int. 2011;60:206-8 pubmed publisher
    Buffalo fasciolosis induced by Fasciola gigantica causes important economic losses in tropical areas of Asia. Detection of prepatent infection is essential to control this disease...
  15. Young N, Jex A, Cantacessi C, Hall R, Campbell B, Spithill T, et al. A portrait of the transcriptome of the neglected trematode, Fasciola gigantica--biological and biotechnological implications. PLoS Negl Trop Dis. 2011;5:e1004 pubmed publisher
    b>Fasciola gigantica (Digenea) is an important foodborne trematode that causes liver fluke disease (fascioliasis) in mammals, including ungulates and humans, mainly in tropical climatic zones of the world...
  16. Choe S, Nguyen T, Kang T, Kweon C, Kang S. Genetic analysis of Fasciola isolates from cattle in Korea based on second internal transcribed spacer (ITS-2) sequence of nuclear ribosomal DNA. Parasitol Res. 2011;109:833-9 pubmed publisher
    ..ITS-2 sequences of 19 Fasciola samples collected from Korean native cattle were determined and compared...
  17. Girones N, Valero M, García Bodelón M, Chico Calero I, Punzon C, Fresno M, et al. Immune suppression in advanced chronic fascioliasis: an experimental study in a rat model. J Infect Dis. 2007;195:1504-12 pubmed
    ..During early chronic infection, there is a predominance of a Th2 response, which decreases in advanced chronic infection characterized by a persistent immune suppression. ..
  18. Rokni M, Mirhendi H, Mizani A, Mohebali M, Sharbatkhori M, Kia E, et al. Identification and differentiation of Fasciola hepatica and Fasciola gigantica using a simple PCR-restriction enzyme method. Exp Parasitol. 2010;124:209-13 pubmed publisher
    Accurate morphological differentiation between the liver fluke species Fasciola hepatica and Fasciola gigantica is difficult...
  19. Xu M, Ai L, Fu J, Nisbet A, Liu Q, Chen M, et al. Comparative characterization of microRNAs from the liver flukes Fasciola gigantica and F. hepatica. PLoS ONE. 2012;7:e53387 pubmed publisher
    ..gigantica and F. hepatica, which has provided novel valuable resources for a better understanding of the two zoonotic trematodes. ..
  20. Golbar H, Izawa T, Juniantito V, Ichikawa C, Tanaka M, Kuwamura M, et al. Immunohistochemical characterization of macrophages and myofibroblasts in fibrotic liver lesions due to Fasciola infection in cattle. J Vet Med Sci. 2013;75:857-65 pubmed
    Histopathologically, fibrosis in Fasciola-infected cattle livers was characterized by inflammatory cell infiltration, such as eosinophils and macrophages, pseudo-lobule, pseudo-bile ducts and fibrotic bridges separating pseudo-lobules; ..
  21. Smith R, Spithill T, Pike R, Meeusen E, Piedrafita D. Fasciola hepatica and Fasciola gigantica: cloning and characterisation of 70 kDa heat-shock proteins reveals variation in HSP70 gene expression between parasite species recovered from sheep. Exp Parasitol. 2008;118:536-42 pubmed publisher
    b>Fasciola hepatica and Fasciola gigantica are trematode parasites responsible for fasciolosis, a disease of ruminant animals which is also increasingly recognised as a disease in humans...
  22. Ingale S, Singh P, Raina O, Mehra U, Verma A, Gupta S, et al. Interferon-gamma and interleukin-4 expression during Fasciola gigantica primary infection in crossbred bovine calves as determined by real -time PCR. Vet Parasitol. 2008;152:158-61 pubmed publisher
    ..interleukin-4 (IL-4) expression in crossbred (Bos taurusxBos indicus) bovine calves during primary infection with Fasciola gigantica was measured...
  23. Walker S, Prodohl P, Hoey E, Fairweather I, Hanna R, Brennan G, et al. Substantial genetic divergence between morphologically indistinguishable populations of Fasciola suggests the possibility of cryptic speciation. Int J Parasitol. 2012;42:1193-9 pubmed publisher
    The liver flukes, Fasciola hepatica and Fasciola gigantica, are considered to be sister species and between them present a major threat worldwide to livestock production...
  24. Valero M, Ubeira F, Khoubbane M, Artigas P, Muiño L, Mezo M, et al. MM3-ELISA evaluation of coproantigen release and serum antibody production in sheep experimentally infected with Fasciola hepatica and F. gigantica. Vet Parasitol. 2009;159:77-81 pubmed publisher
    During an experimental infection of sheep with Fasciola hepatica or F...
  25. Shalaby H, El Namaky A, Kamel R. In vitro effect of artemether and triclabendazole on adult Fasciola gigantica. Vet Parasitol. 2009;160:76-82 pubmed publisher
    ..The tegumental changes occurred following incubation in artemether were comparable with those observed with triclabendazole in its active sulphoxide metabolite form (TCBZ-SX)...
  26. Keyyu J, Kyvsgaard N, Monrad J, Kassuku A. Effectiveness of strategic anthelmintic treatments in the control of gastrointestinal nematodes and Fasciola gigantica in cattle in Iringa region, Tanzania. Trop Anim Health Prod. 2009;41:25-33 pubmed publisher
    ..the effectiveness of strategic anthelmintic treatments in the control of gastrointestinal (GI) nematodes and Fasciola gigantica in cattle...
  27. Anuracpreeda P, Wanichanon C, Chawengkirtikul R, Chaithirayanon K, Sobhon P. Fasciola gigantica: immunodiagnosis of fasciolosis by detection of circulating 28.5 kDa tegumental antigen. Exp Parasitol. 2009;123:334-40 pubmed publisher
    ..5 kDa tegumental antigen (28.5 kDa TA) in the sera from mice experimentally infected with Fasciola gigantica...
  28. Kaset C, Eursitthichai V, Vichasri Grams S, Viyanant V, Grams R. Rapid identification of lymnaeid snails and their infection with Fasciola gigantica in Thailand. Exp Parasitol. 2010;126:482-8 pubmed publisher
    Freshwater snails of the family Lymnaeidae are the intermediate hosts of the liver fluke Fasciola worldwide...
  29. Zhang W, Moreau E, Hope J, Howard C, Huang W, Chauvin A. Fasciola hepatica and Fasciola gigantica: comparison of cellular response to experimental infection in sheep. Exp Parasitol. 2005;111:154-9 pubmed
    Cellular responses to Fasciola gigantica and to Fasciola hepatica infection in sheep were compared. Eosinophil numbers increased more quickly and strongly in F. gigantica-infected sheep than in F. hepatica-infected sheep...
  30. Mas Coma S. Epidemiology of fascioliasis in human endemic areas. J Helminthol. 2005;79:207-16 pubmed
    ..b>Fasciola hepatica has succeeded in expanding from its European original geographical area to colonize five continents, ..
  31. Grams R, Vichasri Grams S, Sobhon P, Upatham E, Viyanant V. Molecular cloning and characterization of cathepsin L encoding genes from Fasciola gigantica. Parasitol Int. 2001;50:105-14 pubmed
    In this study cDNAs encoding cathepsin L-like proteins of Fasciola gigantica were cloned by the reverse transcription polymerase chain reaction method (RT-PCR) from total RNA of adult specimens...
  32. Nguyen T, Le T, Dao T, Tran T, Praet N, Speybroeck N, et al. Bovine fasciolosis in the human fasciolosis hyperendemic Binh Dinh province in Central Vietnam. Acta Trop. 2011;117:19-22 pubmed publisher
    ..In Vietnam, both pure Fasciola gigantica and hybrid and/or introgressed populations of liverflukes bearing genetic material from both Fasciola ..
  33. Smitha S, Raina O, Singh B, Samanta S, Velusamy R, Dangoudoubiyam S, et al. Immune responses to polyethylenimine-mannose-delivered plasmid DNA encoding a Fasciola gigantica fatty acid binding protein in mice. J Helminthol. 2010;84:149-55 pubmed publisher
    b>Fasciola gigantica fatty acid binding protein (FABP) was evaluated for evoking an immune response in mice, by delivering the gene coding for this protein with mannosylated-polyethylenimine (PEI) to peritoneal cells...
  34. Yadav S, Saini M, Raina O, Nambi P, Jadav K, Sriveny D. Fasciola gigantica cathepsin-L cysteine proteinase in the detection of early experimental fasciolosis in ruminants. Parasitol Res. 2005;97:527-34 pubmed
    Cathepsin-L cysteine proteinase was purified from Fasciola gigantica regurgitant by two-step alcoholic fractionation, followed by ion-exchange chromatography...
  35. Molina E, Gonzaga E, Lumbao L. Prevalence of infection with Fasciola gigantica and its relationship to carcase and liver weights, and fluke and egg counts in slaughter cattle and buffaloes in southern Mindanao, Philippines. Trop Anim Health Prod. 2005;37:215-21 pubmed
  36. Pfukenyi D, Mukaratirwa S, Willingham A, Monrad J. Epidemiological studies of Fasciola gigantica infections in cattle in the highveld and lowveld communal grazing areas of Zimbabwe. Onderstepoort J Vet Res. 2006;73:37-51 pubmed
    During the period between January 1999 and December 2000, the distribution and seasonal patterns of Fasciola gigantica infections in cattle in the highveld and lowveld communal grazing areas of Zimbabwe were determined through monthly ..
  37. McGarry J, Ortiz P, Hodgkinson J, Goreish I, Williams D. PCR-based differentiation of Fasciola species (Trematoda: Fasciolidae), using primers based on RAPD-derived sequences. Ann Trop Med Parasitol. 2007;101:415-21 pubmed
    The zoonotic liver flukes Fasciola hepatica and F. gigantica co-exist in parts of Africa and Asia. The two species have similar life-cycles but different transmission characteristics...
  38. Itagaki T, Tsutsumi K. Triploid form of Fasciola in Japan: genetic relationships between Fasciola hepatica and Fasciola gigantica determined by ITS-2 sequence of nuclear rDNA. Int J Parasitol. 1998;28:777-81 pubmed
    The ITS-2 sequences from seven worms of the Japanese triploid form of Fasciola, two of Fasciola hepatica from Uruguay and four of Fasciola gigantica from Zambia and Indonesia were determined...
  39. Ai L, Chen M, Alasaad S, Elsheikha H, Li J, Li H, et al. Genetic characterization, species differentiation and detection of Fasciola spp. by molecular approaches. Parasit Vectors. 2011;4:101 pubmed publisher
    Liver flukes belonging to the genus Fasciola are among the causes of foodborne diseases of parasitic etiology. These parasites cause significant public health problems and substantial economic losses to the livestock industry...
  40. Geadkaew A, von Bülow J, Beitz E, Grams S, Viyanant V, Grams R. Functional analysis of novel aquaporins from Fasciola gigantica. Mol Biochem Parasitol. 2011;175:144-53 pubmed publisher
    Fascioliasis, caused by liver flukes of the genus Fasciola, is an important disease of ruminants. In order to identify a potential new drug target we have studied aquaporin (AQP) in Fasciola gigantica...
  41. McManus D, Dalton J. Vaccines against the zoonotic trematodes Schistosoma japonicum, Fasciola hepatica and Fasciola gigantica. Parasitology. 2006;133 Suppl:S43-61 pubmed
    Schistosoma japonicum, Fasciola hepatica and F. gigantica are digenetic trematodes and, therefore, possess similar life cycles...
  42. Ishii Y, Nakamura Uchiyama F, Nawa Y. A praziquantel-ineffective fascioliasis case successfully treated with triclabendazole. Parasitol Int. 2002;51:205-9 pubmed
    ..5 mg/kg 5 months apart...
  43. Chaithirayanon K, Wanichanon C, Vichasri Grams S, Ardseungneon P, Grams R, Viyanant V, et al. Production and characterization of a monoclonal antibody against 28.5 kDa tegument antigen of Fasciola gigantica. Acta Trop. 2002;84:1-8 pubmed
    A monoclonal antibody (MoAb) against the 28.5 kDa tegumental antigen of Fasciola gigantica was produced by the hybridoma technique using spleen cells from BALB/c mice immunized with the tegumental extract from adult F. gigantica...
  44. Alasaad S, Soriguer R, Abu Madi M, El Behairy A, Jowers M, Baños P, et al. A TaqMan real-time PCR-based assay for the identification of Fasciola spp. Vet Parasitol. 2011;179:266-71 pubmed publisher
    ..In this paper, we report the first qPCR-based assay for the identification of Fasciola spp...
  45. Ai L, Weng Y, Elsheikha H, Zhao G, Alasaad S, Chen J, et al. Genetic diversity and relatedness of Fasciola spp. isolates from different hosts and geographic regions revealed by analysis of mitochondrial DNA sequences. Vet Parasitol. 2011;181:329-34 pubmed publisher
    ..c oxidase subunit 1 (pcox1) and NADH dehydrogenase subunits 4 and 5 (pnad4 and pnad5) among 39 isolates of Fasciola spp...
  46. Soliman M. Epidemiological review of human and animal fascioliasis in Egypt. J Infect Dev Ctries. 2008;2:182-9 pubmed
    ..This article reviews the history, life cycles, transmission, incidence, geographical distribution, and environmental and human determinants that contribute to the epidemiological picture of fascioliasis with special reference to Egypt...
  47. Molina E. Serum interferon-gamma and interleukins-6 and -8 during infection with Fasciola gigantica in cattle and buffaloes. J Vet Sci. 2005;6:135-9 pubmed
    ..of cytokines interferon (IFN)-gamma, interleukins (IL) -6 and -8 in serum of cattle and buffaloes infected with Fasciola gigantica from one to 16 weeks post-infection to determine their T cell response during infection...
  48. Caron Y, Rondelaud D, Losson B. The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp. Parasitol Res. 2008;103:735-44 pubmed publisher
    ..However, at the time being this technique is too expensive to be used routinely in the field. The choice between classical or new techniques is usually based on a compromise, as each technique has its advantages and drawbacks...
  49. Paykari H, Dalimi A, Madani R. Immunization of sheep against Fasciola gigantica with glutathione S-transferase. Vet Parasitol. 2002;105:153-9 pubmed
    Glutathione S-transferase in Fasciola gigantica (FgGST) was isolated by affinity chromatography, by which highly purified enzyme was obtained. FgGST on the SDS-PAGE showed three protein bands ranging 24.5-26.5kDa...
  50. Anuracpreeda P, Chawengkirtikul R, Tinikul Y, Poljaroen J, Chotwiwatthanakun C, Sobhon P. Diagnosis of Fasciola gigantica infection using a monoclonal antibody-based sandwich ELISA for detection of circulating cathepsin B3 protease. Acta Trop. 2013;127:38-45 pubmed publisher
    ..for the detection of circulating cathepsin B3 protease (CatB3) in the sera from mice experimentally infected with Fasciola gigantica and cattle naturally infected with the same parasite...
  51. Irving J, Spithill T, Pike R, Whisstock J, Smooker P. The evolution of enzyme specificity in Fasciola spp. J Mol Evol. 2003;57:1-15 pubmed
    b>Fasciola spp., commonly known as liver fluke, are significant trematode parasites of livestock and humans...
  52. Anderson N, Luong T, Vo N, Bui K, Smooker P, Spithill T. The sensitivity and specificity of two methods for detecting Fasciola infections in cattle. Vet Parasitol. 1999;83:15-24 pubmed
    Counts of Fasciola spp. eggs in faeces and measurements of antibody concentration to the excretory/secretory antigens of Fasciola spp...
  53. Adlard R, Barker S, Blair D, Cribb T. Comparison of the second internal transcribed spacer (ribosomal DNA) from populations and species of Fasciolidae (Digenea). Int J Parasitol. 1993;23:423-5 pubmed
    ..of the 3' end of the second internal transcribed spacer region (ITS2) was determined for four isolates of Fasciola hepatica, two isolates of Fasciola gigantica, one isolate of Fascioloides magna and one isolate of Fasciola sp...
  54. Chunchob S, Grams R, Viyanant V, Smooker P, Vichasri Grams S. Comparative analysis of two fatty acid binding proteins from Fasciola gigantica. Parasitology. 2010;137:1805-17 pubmed publisher
    ..In order to provide additional information about their function in Fasciola gigantica we performed a comparative analysis of FgFABP1 and FgFABP3, two isoforms with quite different ..
  55. Itagaki T, Kikawa M, Sakaguchi K, Shimo J, Terasaki K, Shibahara T, et al. Genetic characterization of parthenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA. Parasitology. 2005;131:679-85 pubmed
    Accurate identification of aspermic Fasciola forms in Japan remains difficult because of their morphological variations...
  56. Anuracpreeda P, Wanichanon C, Sobhon P. Fasciola gigantica: immunolocalization of 28.5 kDa antigen in the tegument of metacercaria and juvenile fluke. Exp Parasitol. 2009;122:75-83 pubmed publisher
    ..this antigen in the tissues of metacercariae, newly excysted juvenile (NEJ), 1, 3, 5, and 7-week-old juveniles of Fasciola gigantica by using indirect immunofluorescence, immunoperoxidase and immunogold techniques...
  57. Nguyen S, Nguyen D, Van Nguyen T, Huynh V, Le D, Fukuda Y, et al. Prevalence of Fasciola in cattle and of its intermediate host Lymnaea snails in central Vietnam. Trop Anim Health Prod. 2012;44:1847-53 pubmed publisher
    The aims of this study were to investigate the prevalence of natural Fasciola infections in both the definitive hosts (cattle) and the intermediate hosts (Lymnaea snails) in central Vietnam...
  58. Intapan P, Maleewong W, Wongkham C, Tomanakarn K, Ieamviteevanich K, Pipitgool V, et al. Excretory-secretory antigenic components of adult Fasciola gigantica recognized by infected human sera. Southeast Asian J Trop Med Public Health. 1998;29:579-83 pubmed
    The immunogenic components of Fasciola gigantica excretory-secretory (ES) products were revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting technic using sera from patients with F...
  59. Ai L, Li C, Elsheikha H, Hong S, Chen J, Chen S, et al. Rapid identification and differentiation of Fasciola hepatica and Fasciola gigantica by a loop-mediated isothermal amplification (LAMP) assay. Vet Parasitol. 2010;174:228-33 pubmed publisher
    ..loop-mediated isothermal amplification (LAMP) assay for the rapid detection and discrimination of Fasciola hepatica and Fasciola gigantica...
  60. Moghaddam A, Massoud J, Mahmoodi M, Mahvi A, Periago M, Artigas P, et al. Human and animal fascioliasis in Mazandaran province, northern Iran. Parasitol Res. 2004;94:61-9 pubmed
    ..Morphometric studies by computer image analysis showed that forms intermediate between Fasciola hepatica and F. gigantica appear in addition to both species...
  61. Ali H, Ai L, Song H, Ali S, Lin R, Seyni B, et al. Genetic characterisation of Fasciola samples from different host species and geographical localities revealed the existence of F. hepatica and F. gigantica in Niger. Parasitol Res. 2008;102:1021-4 pubmed publisher
    In the present study, 16 samples representing Fasciola (Platyhelminthes: Trematoda: Digenea) from sheep and cattle from seven geographical locations in Niger were characterized genetically by sequences of the first (ITS-1) and second (ITS-..
  62. Nguyen T, van De N, Vercruysse J, Dorny P, Le T. Genotypic characterization and species identification of Fasciola spp. with implications regarding the isolates infecting goats in Vietnam. Exp Parasitol. 2009;123:354-61 pubmed publisher
    ..or 362bp) of the second internal transcribed spacer 2 (ITS-2) and a portion of mitochondrial cox1 (423bp) for Fasciola spp...
  63. Valero M, Perez Crespo I, Periago M, Khoubbane M, Mas Coma S. Fluke egg characteristics for the diagnosis of human and animal fascioliasis by Fasciola hepatica and F. gigantica. Acta Trop. 2009;111:150-9 pubmed publisher
    ..The aim of the present study is to validate the identification of Fasciola species based on the shape and size of eggs shed by humans, characterizing their morphometric traits using a ..
  64. Tansatit T, Sahaphong S, Riengrojpitak S, Viyanant V, Sobhon P. Fasciola gigantica: the in vitro effects of artesunate as compared to triclabendazole on the 3-weeks-old juvenile. Exp Parasitol. 2012;131:8-19 pubmed publisher
    The in vitro effect of artesunate (ATS) on the 3-week-old juveniles of Fasciola gigantica was compared with triclabendazole (TCZ) by incubating the parasites in M-199 medium containing the drugs at concentrations of 20, 40, and 80 ?g/ml ..
  65. Subpipattana P, Grams R, Vichasri Grams S. Analysis of a calcium-binding EF-hand protein family in Fasciola gigantica. Exp Parasitol. 2012;130:364-73 pubmed publisher
    ..In the present study, we have compared three homologs of the liver fluke Fasciola gigantica for their immunological properties in mouse...
  66. Sirisriro A, Grams R, Vichasri Grams S, Ardseungneon P, Pankao V, Meepool A, et al. Production and characterization of a monoclonal antibody against recombinant fatty acid binding protein of Fasciola gigantica. Vet Parasitol. 2002;105:119-29 pubmed
    In Fasciola parasites fatty acid binding proteins (FABPs) are the carrier proteins that help in the uptake of fatty acids from the hosts' fluids...
  67. Rokni M, Massoud J, Hanilo A. Comparison of adult somatic and cysteine proteinase antigens of Fasciola gigantica in enzyme linked immunosorbent assay for serodiagnosis of human fasciolosis. Acta Trop. 2003;88:69-75 pubmed
    Fasciolosis caused by Fasciola hepatica and Fasciola gigantica is one of the major public health problems in the world and in Iran...
  68. Mungube E, Bauni S, Tenhagen B, Wamae L, Nginyi J, Mugambi J. The prevalence and economic significance of Fasciola gigantica and Stilesia hepatica in slaughtered animals in the semi-arid coastal Kenya. Trop Anim Health Prod. 2006;38:475-83 pubmed
    b>Fasciola gigantica and Stilesia hepatica constrain ruminant productivity. The prevalence and economic losses caused by F. gigantica and S...
  69. Caron Y, Righi S, Lempereur L, Saegerman C, Losson B. An optimized DNA extraction and multiplex PCR for the detection of Fasciola sp. in lymnaeid snails. Vet Parasitol. 2011;178:93-9 pubmed publisher
    This study deals with the development and validation of an original PCR protocol to assess the presence of Fasciola hepatica in Galba truncatula its main intermediate host in Western Europe...
  70. El Morshedy H, Shehab A, Zaki A, Farag H. Intra-specimen and day-to-day variations of Fasciola egg counts in human stools. East Mediterr Health J. 2002;8:619-25 pubmed
    Intra-specimen and day-to-day variations of Fasciola egg counts in stools were investigated for 16 cases of established fascioliasis. For each case six Kato slides from a single stool sample were examined daily for 5 consecutive days...
  71. Ichikawa M, Itagaki T. Discrimination of the ITS1 types of Fasciola spp. based on a PCR-RFLP method. Parasitol Res. 2010;106:757-61 pubmed publisher
    Molecular characterization is important for discriminating Fasciola specimens having the deoxyribonucleic acid (DNA) sequences of Fasciola hepatica, Fasciola gigantica, and both Fasciola species, since three Fasciola forms coexist in ..
  72. Abou Zinadah N, Fouad M. Anti-Fasciola antibodies among rodents and sheep in Jeddah, Saudi Arabia. J Egypt Soc Parasitol. 2005;35:711-6 pubmed
    A total of 20 imported sheep were examined for fascioliasis natural infection by kato thick smear and by Fasciola-indirect haemagglutination test (IHAT). Stool examination revealed infection in 13/20 (65%), but IHAT identified 11/20 (55%)...
  73. Intapan P, Tantrawatpan C, Maleewong W, Wongkham S, Wongkham C, Nakashima K. Potent epitopes derived from Fasciola gigantica cathepsin L1 in peptide-based immunoassay for the serodiagnosis of human fascioliasis. Diagn Microbiol Infect Dis. 2005;53:125-9 pubmed
    ..assay (ELISA) was developed and evaluated for its diagnostic ability to detect human IgG antibodies against Fasciola gigantica cathepsin L1...
  74. Raadsma H, Kingsford N, Suharyanta -, Spithill T, Piedrafita D. Host responses during experimental infection with Fasciola gigantica and Fasciola hepatica in Merino sheep II. Development of a predictive index for Fasciola gigantica worm burden. Vet Parasitol. 2008;154:250-61 pubmed publisher
    ..immunological and pathological responses following experimental inoculation with incremental doses of Fasciola gigantica in sheep...
  75. Le T, De N, Agatsuma T, Blair D, Vercruysse J, Dorny P, et al. Molecular confirmation that Fasciola gigantica can undertake aberrant migrations in human hosts. J Clin Microbiol. 2007;45:648-50 pubmed
    Two cases of aberrant migration by the liver fluke Fasciola gigantica in humans are reported. In both cases, subadult worms emerged through the skin. The identity of the worms was confirmed from their DNA sequences...
  76. Amor N, Farjallah S, Salem M, Lamine D, Merella P, Said K, et al. Molecular characterization of Fasciola gigantica from Mauritania based on mitochondrial and nuclear ribosomal DNA sequences. Exp Parasitol. 2011;129:127-36 pubmed publisher
    Fasciolosis caused by Fasciola hepatica and Fasciola gigantica (Platyhelminthes: Trematoda: Digenea) is considered the most important helminth infection of ruminants in tropical countries, causing considerable socioeconomic problems...
  77. Tantrawatpan C, Maleewong W, Wongkham C, Wongkham S, Intapan P, Nakashima K. Characterisation of Fasciola gigantica adult 27-kDa excretory-secretory antigen in human fascioliasis. Parasitol Res. 2003;91:325-7 pubmed
    The 27-kDa excretory-secretory antigen partially purified from adult Fasciola gigantica adult worms (FG27) has been used as the sensitive and specific antigen for immunodiagnosis of human fascioliasis...
  78. Meemon K, Khawsuk W, Sriburee S, Meepool A, Sethadavit M, Sansri V, et al. Fasciola gigantica: histology of the digestive tract and the expression of cathepsin L. Exp Parasitol. 2010;125:371-9 pubmed publisher
    The digestive tract of Fasciola gigantica is composed of the oral sucker, buccal tube, pharynx, esophagus, and caecum...
  79. Ai L, Dong S, Zhang W, Elsheikha H, Mahmmod Y, Lin R, et al. Specific PCR-based assays for the identification of Fasciola species: their development, evaluation and potential usefulness in prevalence surveys. Ann Trop Med Parasitol. 2010;104:65-72 pubmed publisher
    Among the helminths infecting ruminants in China are three taxa belonging to the genus Fasciola: F. hepatica, F. gigantica and the so-called 'intermediate form' that appears to lie between these two species...
  80. Molina E, Skerratt L. Cellular and humoral responses in liver of cattle and buffaloes infected with a single dose of Fasciola gigantica. Vet Parasitol. 2005;131:157-63 pubmed
    ..components of the hepatic inflammatory infiltrate in cattle and buffaloes infected with a single dose of 1000 Fasciola gigantica were analysed by immunohistochemistry and histology...
  81. Periago M, Valero M, el Sayed M, Ashrafi K, el Wakeel A, Mohamed M, et al. First phenotypic description of Fasciola hepatica/Fasciola gigantica intermediate forms from the human endemic area of the Nile Delta, Egypt. Infect Genet Evol. 2008;8:51-8 pubmed
    b>Fasciola gigantica is the main fasciolid species in Africa; however, F. hepatica and F. gigantica overlap in some countries. Egypt deserves mentioning because of the emerging situation of human fascioliasis in the Nile Delta area...
  82. Piedrafita D, Estuningsih E, Pleasance J, Prowse R, Raadsma H, Meeusen E, et al. Peritoneal lavage cells of Indonesian thin-tail sheep mediate antibody-dependent superoxide radical cytotoxicity in vitro against newly excysted juvenile Fasciola gigantica but not juvenile Fasciola hepatica. Infect Immun. 2007;75:1954-63 pubmed
    Indonesian thin-tail (ITT) sheep resist infection by Fasciola gigantica by an immunological mechanism within 2 to 4 weeks of infection yet are susceptible to F. hepatica infection...