brugia malayi

Summary

Summary: A species of parasitic nematode causing Malayan filariasis and having a distribution centering roughly on the Malay peninsula. The life cycle of B. malayi is similar to that of WUCHERERIA BANCROFTI, except that in most areas the principal mosquito vectors belong to the genus Mansonia.

Top Publications

  1. Landmann F, Voronin D, Sullivan W, Taylor M. Anti-filarial activity of antibiotic therapy is due to extensive apoptosis after Wolbachia depletion from filarial nematodes. PLoS Pathog. 2011;7:e1002351 pubmed publisher
  2. Foster J, Ganatra M, Kamal I, Ware J, Makarova K, Ivanova N, et al. The Wolbachia genome of Brugia malayi: endosymbiont evolution within a human pathogenic nematode. PLoS Biol. 2005;3:e121 pubmed
    ..endosymbiont required for fertility and survival of the human filarial parasitic nematode Brugia malayi. Although, quantitatively, the genome is even more degraded than those of closely related Rickettsia species, ..
  3. Spencer L, Shultz L, Rajan T. T cells are required for host protection against Brugia malayi but need not produce or respond to interleukin-4. Infect Immun. 2003;71:3097-106 pubmed
    T cells are known to be required for host protection in mouse models of Brugia malayi infection. Several independent studies in murine models have also highlighted the rapid induction of Th2-like responses after infection with B...
  4. Rao R, Huang Y, Abubucker S, Heinz M, Crosby S, Mitreva M, et al. Effects of doxycycline on gene expression in Wolbachia and Brugia malayi adult female worms in vivo. J Biomed Sci. 2012;19:21 pubmed publisher
    ..of this study was to examine the effects of doxycycline on gene expression in Wolbachia and adult female Brugia malayi. Brugia malayi infected gerbils were treated with doxycycline for 6-weeks...
  5. Intapan P, Thanchomnang T, Lulitanond V, Maleewong W. Rapid detection of Wuchereria bancrofti and Brugia malayi in mosquito vectors (Diptera: Culicidae) using a real-time fluorescence resonance energy transfer multiplex PCR and melting curve analysis. J Med Entomol. 2009;46:158-64 pubmed
    ..chain reaction (PCR) merged with melting curve analysis for the detection of Wuchereria bancrofti and Brugia malayi DNA in blood-fed mosquitoes...
  6. Mand S, Supali T, Djuardi J, Kar S, Ravindran B, Hoerauf A. Detection of adult Brugia malayi filariae by ultrasonography in humans in India and Indonesia. Trop Med Int Health. 2006;11:1375-81 pubmed
    ..In this study, we describe the feasibility of detection of adult Brugia malayi filariae by USG...
  7. McSorley H, Harcus Y, Murray J, Taylor M, Maizels R. Expansion of Foxp3+ regulatory T cells in mice infected with the filarial parasite Brugia malayi. J Immunol. 2008;181:6456-66 pubmed
    Many helminths, including Brugia malayi, are able to establish long-lived infections in immunocompetent hosts...
  8. McGarry H, Egerton G, Taylor M. Population dynamics of Wolbachia bacterial endosymbionts in Brugia malayi. Mol Biochem Parasitol. 2004;135:57-67 pubmed
    The human filarial nematode Brugia malayi contains an endosymbiotic bacterium, Wolbachia...
  9. Simons J, Rothenberg M, Lawrence R. Eotaxin-1-regulated eosinophils have a critical role in innate immunity against experimental Brugia malayi infection. Eur J Immunol. 2005;35:189-97 pubmed
    Using two models of filarial infection in which Brugia malayi microfilariae (Mf) are contained in distinct anatomical compartments, in blood or tissue sites, we have demonstrated a critical role for eotaxin-1 in parasite clearance...

More Information

Publications165 found, 100 shown here

  1. Chai J, Lee S, Choi S, Lee J, Yong T, Park K, et al. A survey of Brugia malayi infection on the Heugsan Islands, Korea. Korean J Parasitol. 2003;41:69-73 pubmed
    Lymphatic filariasis due to Brugia malayi infection was endemic in several areas of South Korea...
  2. Aliota M, Fuchs J, Mayhew G, Chen C, Christensen B. Mosquito transcriptome changes and filarial worm resistance in Armigeres subalbatus. BMC Genomics. 2007;8:463 pubmed
    ..subalbatus is a natural vector of the filarial worm Brugia pahangi, but it rapidly and proficiently kills Brugia malayi microfilariae by melanotic encapsulation. Because B. malayi and B...
  3. Vedi S, Dangi A, Hajela K, Misra Bhattacharya S. Vaccination with 73kDa recombinant heavy chain myosin generates high level of protection against Brugia malayi challenge in jird and mastomys models. Vaccine. 2008;26:5997-6005 pubmed publisher
    We have earlier reported identification, expression and purification of a 2.0kb cDNA clone coding for Brugia malayi heavy chain myosin which exhibited strong immuno-reactivity with bancroftian sera from endemic normal (EN) human subjects ..
  4. Misra R, Verma A, Verma S, Kumar V, Siddiqui W, Siddiqi M, et al. Heat shock protein 60 of filarial parasite Brugia malayi: cDNA cloning, expression, purification and in silico modeling and analysis of its ATP binding site. Exp Parasitol. 2012;132:257-66 pubmed publisher
    We report here cloning and expression of full length mitochondrial HSP60 gene of Brugia malayi adult worm (mtHSP60bm), purification of the gene product by affinity chromatography, its in silico 3D structure and the sequence homology of ..
  5. Lakshmi V, Joseph S, Srivastava S, Verma S, Sahoo M, Dube V, et al. Antifilarial activity in vitro and in vivo of some flavonoids tested against Brugia malayi. Acta Trop. 2010;116:127-33 pubmed publisher
    We evaluated the antifilarial activity of 6 flavonoids against the human lymphatic filarial parasite Brugia malayi using an in vitro motility assay with adult worms and microfilariae, a biochemical test for viability (3-(4,5-..
  6. Li B, Rush A, Jiang D, Mitreva M, Abubucker S, Weil G. Gender-associated genes in filarial nematodes are important for reproduction and potential intervention targets. PLoS Negl Trop Dis. 2011;5:e947 pubmed publisher
    ..18,104 elements representing ?85% of the filarial genome was used to identify GA gene transcripts in adult Brugia malayi worms. Approximately 19% of 14,293 genes were identified as GA genes...
  7. Nair M, Cochrane D, Allen J. Macrophages in chronic type 2 inflammation have a novel phenotype characterized by the abundant expression of Ym1 and Fizz1 that can be partly replicated in vitro. Immunol Lett. 2003;85:173-80 pubmed
    ..In future, we hope to use in vitro analysis to help define the pathways that lead to this distinctive in vivo macrophage phenotype. ..
  8. Fenn K, Blaxter M. Quantification of Wolbachia bacteria in Brugia malayi through the nematode lifecycle. Mol Biochem Parasitol. 2004;137:361-4 pubmed
  9. Mand S, Specht S, Zahner H, Hoerauf A. Ultrasonography in filaria-infected rodents: detection of adult Litomosoides sigmodontis and Brugia malayi filariae. Trop Med Int Health. 2006;11:1382-7 pubmed
    ..Wuchereria bancrofti are frequently detected using USG in humans, whereas adult Brugia malayi have not been so far...
  10. Mayhew G, Bartholomay L, Kou H, Rocheleau T, Fuchs J, Aliota M, et al. Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus. BMC Genomics. 2007;8:462 pubmed
    ..Armigeres subalbatus, mounts a distinctively robust innate immune response when infected with the nematode Brugia malayi, a causative agent of lymphatic filariasis...
  11. Sahare K, Anandharaman V, Meshram V, Meshram S, Gajalakshmi D, Goswami K, et al. In vitro effect of four herbal plants on the motility of Brugia malayi microfilariae. Indian J Med Res. 2008;127:467-71 pubmed
    ..Usage of herbal drugs in traditional medicine is quite well known but largely empirical. Hence the present study was designed to screen the in vitro antifilarial effect of four herbal plants on Brugia malayi.
  12. Fischer P, Erickson S, Fischer K, Fuchs J, Rao R, Christensen B, et al. Persistence of Brugia malayi DNA in vector and non-vector mosquitoes: implications for xenomonitoring and transmission monitoring of lymphatic filariasis. Am J Trop Med Hyg. 2007;76:502-7 pubmed
    ..mf) and filarial DNA in vector competent and non-competent mosquito strains, we compared the detection of Brugia malayi parasites by dissection and by quantitative real-time polymerase chain reaction (PCR) in three different ..
  13. Mylonas K, Nair M, Prieto Lafuente L, Paape D, Allen J. Alternatively activated macrophages elicited by helminth infection can be reprogrammed to enable microbial killing. J Immunol. 2009;182:3084-94 pubmed publisher
    ..However, the adaptation to antimicrobial function did not extend to key regulatory pathways, such as IL-12 production, which remained unaltered...
  14. Xu S, Liu C, Tzertzinis G, Ghedin E, Evans C, Kaplan R, et al. In vivo transfection of developmentally competent Brugia malayi infective larvae. Int J Parasitol. 2011;41:355-62 pubmed publisher
    Transient transfection of isolated Brugia malayi embryos by biolistics has proven to be useful in defining promoter structure and function in this parasite. However, isolated transfected embryos are developmentally incompetent...
  15. Singh M, Shakya S, Soni V, Dangi A, Kumar N, Bhattacharya S. The n-hexane and chloroform fractions of Piper betle L. trigger different arms of immune responses in BALB/c mice and exhibit antifilarial activity against human lymphatic filarid Brugia malayi. Int Immunopharmacol. 2009;9:716-28 pubmed publisher
    ..specific immune responses in another rodent Mastomys coucha infected with human lymphatic filarial parasite Brugia malayi. The crude methanol extract and n-hexane fraction were found to potentiate significant (p<0...
  16. Taylor M, Hoerauf A. Wolbachia bacteria of filarial nematodes. Parasitol Today. 1999;15:437-42 pubmed
  17. Williamson S, Walsh T, Wolstenholme A. The cys-loop ligand-gated ion channel gene family of Brugia malayi and Trichinella spiralis: a comparison with Caenorhabditis elegans. Invert Neurosci. 2007;7:219-26 pubmed
    ..elegans receptors but to date no genome wide investigations have been done. The genome sequencing projects of Brugia malayi (clade III) and Trichinella spiralis (clade I) have allowed us to characterise the CLGIC families in these ..
  18. Foster J, Raverdy S, Ganatra M, Colussi P, Taron C, Carlow C. The Wolbachia endosymbiont of Brugia malayi has an active phosphoglycerate mutase: a candidate target for anti-filarial therapies. Parasitol Res. 2009;104:1047-52 pubmed publisher
    ..gene (iPGM) was identified in the genome sequence of the Wolbachia endosymbiont from the filarial nematode, Brugia malayi (wBm)...
  19. Poole C, Davis P, Jin J, McReynolds L. Cloning and bioinformatic identification of small RNAs in the filarial nematode, Brugia malayi. Mol Biochem Parasitol. 2010;169:87-94 pubmed publisher
    Characterization of small RNAs from the filarial nematode Brugia malayi is the initial step in understanding their role in gene silencing...
  20. Krishna N, Krushna N, Narayanan R, Rajan S, Gunasekaran K. Expression, purification and characterization of refolded rBm-33 (pepsin inhibitor homolog) from Brugia malayi: a human Lymphatic Filarial parasite. Protein Expr Purif. 2011;79:245-50 pubmed publisher
    Bm-33 (pepsin inhibitor homolog) produced by the human filarial parasite Brugia malayi, was expressed in Escherichia coli...
  21. Gnanasekar M, Ramaswamy K. Translationally controlled tumor protein of Brugia malayi functions as an antioxidant protein. Parasitol Res. 2007;101:1533-40 pubmed
    ..In the present work, we report the putative antioxidant function of Brugia malayi TCTP (BmTCTP)...
  22. Tompkins J, Stitt L, Ardelli B. Brugia malayi: in vitro effects of ivermectin and moxidectin on adults and microfilariae. Exp Parasitol. 2010;124:394-402 pubmed publisher
    The effect of ivermectin and moxidectin on the motility of Brugia malayi adults and microfilariae and on the fertility of B. malayi females was examined...
  23. Aliota M, Fuchs J, Rocheleau T, Clark A, HILLYER J, Chen C, et al. Mosquito transcriptome profiles and filarial worm susceptibility in Armigeres subalbatus. PLoS Negl Trop Dis. 2010;4:e666 pubmed publisher
    Armigeres subalbatus is a natural vector of the filarial worm Brugia pahangi, but it kills Brugia malayi microfilariae by melanotic encapsulation. Because B. malayi and B...
  24. Krushna N, Shiny C, Dharanya S, Sindhu A, Aishwarya S, Narayanan R. Immunolocalization and serum antibody responses to Brugia malayi pepsin inhibitor homolog (Bm-33). Microbiol Immunol. 2009;53:173-83 pubmed publisher
    cDNA coding for Brugia malayi pepsin inhibitor homolog (Bm-33) from the human filarial parasite was cloned in pRSET for large-scale expression and functional characterization...
  25. Bag S, Tawari N, Sharma R, Goswami K, Reddy M, Degani M. In vitro biological evaluation of biguanides and dihydrotriazines against Brugia malayi and folate reversal studies. Acta Trop. 2010;113:48-51 pubmed publisher
    ..with the antimalarial drug pyrimethamine and the antibacterial drug trimethoprim in an in vitro model against Brugia malayi microfilariae (Mf)...
  26. Srivastava Y, Rathaur S, Bhandari Y, Reddy M, Harinath B. Adult 175 kDa collagenase antigen of Setaria cervi in immunoprophylaxis against Brugia malayi in jirds. J Helminthol. 2004;78:347-52 pubmed
    ..was undertaken on the immunoprophylactic potential of 175 kDa collagenase in inducing immunity against Brugia malayi (a human filarial parasite) in jirds (Meriones unguiculatus) in vitro and in situ...
  27. Ramachandran S, Kumar M, Rami R, Chinnaiah H, Nutman T, Kaliraj P, et al. The larval specific lymphatic filarial ALT-2: induction of protection using protein or DNA vaccination. Microbiol Immunol. 2004;48:945-55 pubmed
    ..02 for protein and P=0.01 for the DNA vaccine) or compared to adjuvant alone. This level of protection indicates that this vaccine is a promising candidate for further development. ..
  28. Porksakorn C, Nuchprayoon S, Park K, Scott A. Proinflammatory cytokine gene expression by murine macrophages in response to Brugia malayi Wolbachia surface protein. Mediators Inflamm. 2007;2007:84318 pubmed
    ..These results strongly suggest that the major Wolbachia surface protein molecule WSP is an important inducer of innate immune responses during filarial infections. ..
  29. Dabir S, Dabir P, Goswami K, Goswamy K, Reddy M. Prophylactic evaluation of recombinant extracellular superoxide dismutase of Brugia malayi in jird model. Vaccine. 2008;26:3705-10 pubmed publisher
    The immunoscreening of Brugia malayi adult cDNA library with pooled endemic normal sera identified several seroreactive clones including, EC-SOD which contained a 612 bp insert and showed significant nucleotide and deduced amino acid ..
  30. Singh A, Joshi S, Arya R, Kayastha A, Saxena J. Guanidine hydrochloride and urea-induced unfolding of Brugia malayi hexokinase. Eur Biophys J. 2010;39:289-97 pubmed publisher
  31. Griffiths K, Mayhew G, Zink R, Erickson S, Fuchs J, McDermott C, et al. Use of microarray hybridization to identify Brugia genes involved in mosquito infectivity. Parasitol Res. 2009;106:227-35 pubmed publisher
    b>Brugia malayi and Brugia pahangi microfilariae (mf) require a maturation period of at least 5 days in the mammalian host to successfully infect laboratory mosquitoes...
  32. Krishna N, Krushna N, Narayanan R, Rajan S, Gunasekaran K. Physicochemical characterization of an aspin (rBm-33) from a filarial parasite Brugia malayi against the important human aspartic proteases. J Enzyme Inhib Med Chem. 2013;28:1054-60 pubmed publisher
    The aspartic protease inhibitory efficiency of rBm-33, an aspin from a filarial parasite Brugia malayi was investigated. rBm-33 was found to be thermostable up to 90°C and it forms a stable 'enzyme-product' complex with human pepsin...
  33. Zang X, Taylor P, Wang J, Meyer D, Scott A, Walkinshaw M, et al. Homologues of human macrophage migration inhibitory factor from a parasitic nematode. Gene cloning, protein activity, and crystal structure. J Biol Chem. 2002;277:44261-7 pubmed
    ..the human cytokine macrophage migration inhibitory factor (MIF) have been isolated from the parasitic nematode Brugia malayi. Both molecules (Bm-MIF-1 and Bm-MIF-2) show parallel functions to human MIF...
  34. Cheun H, Lee J, Cho S, Kong Y, Kim T. Elimination of lymphatic filariasis in the Republic of Korea: an epidemiological survey of formerly endemic areas, 2002-2006. Trop Med Int Health. 2009;14:445-9 pubmed publisher
    ..for microfilaria testing and assayed samples from 3049 children (10- to 13-year-olds) and 1526 adults for Brugia malayi antibodies. We found two cases (0...
  35. Dakshinamoorthy G, Samykutty A, Munirathinam G, Shinde G, Nutman T, Reddy M, et al. Biochemical characterization and evaluation of a Brugia malayi small heat shock protein as a vaccine against lymphatic filariasis. PLoS ONE. 2012;7:e34077 pubmed publisher
    ..Recently, we identified a small heat shock protein expressed by Brugia malayi (BmHsp12...
  36. Zahner H, Taubert A, Harder A, von Samson Himmelstjerna G. Effects of Bay 44-4400, a new cyclodepsipeptide, on developing stages of filariae (Acanthocheilonema viteae, Brugia malayi, Litomosoides sigmodontis) in the rodent Mastomys coucha. Acta Trop. 2001;80:19-28 pubmed
    ..as a spot on formulation and administered in single doses of 25 and 100 mg/kg to Acanthocheilonema viteae, Brugia malayi, and Litomosoides sigmodontis infected Mastomys coucha on various dates during prepatency, aiming to affect ..
  37. Dixit S, Gaur R, Sahoo M, Joseph S, Murthy P, Murthy P. Protection against L3 induced Brugia malayi infection in Mastomys coucha pre-immunized with BmAFII fraction of the filarial adult worm. Vaccine. 2006;24:5824-31 pubmed
    The present study was aimed at investigating protective efficacy of BmAFII (Sephadex G-200 eluted fraction of Brugia malayi adult worm extract) against establishment of infective larvae (L3)-induced B...
  38. Shenoy R, Suma T, Kumaraswami V, Rahmah N, Dhananjayan G, Padma S, et al. Preliminary findings from a cross-sectional study on lymphatic filariasis in children, in an area of India endemic for Brugia malayi infection. Ann Trop Med Parasitol. 2007;101:205-13 pubmed
    ..Even these preliminary results have important implications for filariasis-control programmes and emphasise the need for disability-alleviation efforts among children as well as adults. ..
  39. Supali T, Djuardi Y, Pfarr K, Wibowo H, Taylor M, Hoerauf A, et al. Doxycycline treatment of Brugia malayi-infected persons reduces microfilaremia and adverse reactions after diethylcarbamazine and albendazole treatment. Clin Infect Dis. 2008;46:1385-93 pubmed publisher
    The efficacy of doxycycline for treating the causal agent of human lymphatic filariasis, Brugia malayi, is unknown. Standard treatment with diethylcarbamazine-albendazole is associated with adverse reactions...
  40. Blaxter M, Daub J, Guiliano D, Parkinson J, Whitton C. The Brugia malayi genome project: expressed sequence tags and gene discovery. Trans R Soc Trop Med Hyg. 2002;96:7-17 pubmed
    To advance and facilitate molecular studies of Brugia malayi, one of the causative agents of human lymphatic filariasis, an expressed sequence tag (EST)-based gene discovery programme has been carried out...
  41. Wongkamchai S, Chiangjong W, Sinchaikul S, Chen S, Choochote W, Thongboonkerd V. Identification of Brugia malayi immunogens by an immunoproteomics approach. J Proteomics. 2011;74:1607-13 pubmed publisher
    ..We applied immunoproteomics to define potential immunogens of adult Brugia malayi that were recognized by IgM, IgG1 and IgG4 in sera of patients with four distinct clinical spectra of ..
  42. Falcone F, Loke P, Zang X, MacDonald A, Maizels R, Allen J. A Brugia malayi homolog of macrophage migration inhibitory factor reveals an important link between macrophages and eosinophil recruitment during nematode infection. J Immunol. 2001;167:5348-54 pubmed
    Infections with the helminth parasite Brugia malayi share many key features with Th2-mediated allergic diseases, including recruitment of eosinophils...
  43. Gupta R, Tyagi K, Jain S, Misra Bhattacharya S. Brugia malayi: establishment in inbred and outbred strains of mice. Exp Parasitol. 2003;103:57-60 pubmed
    Immunocompetent mouse model for human filarial parasite Brugia malayi is urgently required in view of the paucity of commercial reagents for other susceptible rodent viz. mastomys and gerbil...
  44. Punkosdy G, Addiss D, Lammie P. Characterization of antibody responses to Wolbachia surface protein in humans with lymphatic filariasis. Infect Immun. 2003;71:5104-14 pubmed
  45. Sukuru S, Crepin T, Milev Y, Marsh L, Hill J, Anderson R, et al. Discovering new classes of Brugia malayi asparaginyl-tRNA synthetase inhibitors and relating specificity to conformational change. J Comput Aided Mol Des. 2006;20:159-78 pubmed
    ..and ligand side chains while docking, was used to screen several large databases to identify inhibitors of Brugia malayi asparaginyl-tRNA synthetase (AsnRS), a target for anti-parasitic drug design...
  46. Li B, Rush A, Mitreva M, Yin Y, Spiro D, Ghedin E, et al. Transcriptomes and pathways associated with infectivity, survival and immunogenicity in Brugia malayi L3. BMC Genomics. 2009;10:267 pubmed publisher
    ..This study explores changes in gene expression associated with the transition of Brugia malayi third stage larvae (BmL3) from mosquitoes into mammalian hosts and how these changes are affected by radiation...
  47. Shakya S, Singh P, Kushwaha S, Misra Bhattacharya S. Adult Brugia malayi approximately 34 kDa (BMT-5) antigen offers Th1 mediated significant protection against infective larval challenge in Mastomys coucha. Parasitol Int. 2009;58:346-53 pubmed publisher
    ..The findings suggest that approximately 34 kDa (BMT-5) molecules present in the MT fraction of adult B. malayi provided sizeable protection against infective larval challenge by generating a Th1 biased milieu in the host. ..
  48. Gomez Escobar N, Bennett C, Prieto Lafuente L, Aebischer T, Blackburn C, Maizels R. Heterologous expression of the filarial nematode alt gene products reveals their potential to inhibit immune function. BMC Biol. 2005;3:8 pubmed
    ..We investigated two such novel genes from Brugia malayi termed abundant larval transcript (alt), expression of which reaches ~5% of total transcript at the time ..
  49. Crossgrove K, Maina C, Robinson Rechavi M, Lochner M. Orthologues of the Drosophila melanogaster E75 molting control gene in the filarial parasites Brugia malayi and Dirofilaria immitis. Mol Biochem Parasitol. 2008;157:92-7 pubmed
    Filarial parasites cause debilitating diseases in humans and domesticated animals. Brugia malayi and Dirofilaria immitis are transmitted by mosquitoes and infect humans and dogs, respectively...
  50. Shakya S, Bajpai P, Sharma S, Misra Bhattacharya S. Prior killing of intracellular bacteria Wolbachia reduces inflammatory reactions and improves antifilarial efficacy of diethylcarbamazine in rodent model of Brugia malayi. Parasitol Res. 2008;102:963-72 pubmed publisher
    ..using a rodent model Mastomys coucha and Meriones unguiculatus infected with human lymphatic filariid Brugia malayi. We observed: (1) a 40-day treatment schedule of tetracycline alone resulted in delayed reduction in ..
  51. Turner J, Langley R, Johnston K, Egerton G, Wanji S, Taylor M. Wolbachia endosymbiotic bacteria of Brugia malayi mediate macrophage tolerance to TLR- and CD40-specific stimuli in a MyD88/TLR2-dependent manner. J Immunol. 2006;177:1240-9 pubmed
    ..We pre-exposed murine peritoneal-elicited macrophages to soluble extracts of Brugia malayi female worms (BMFE) before restimulating with BMFE or TLR agonists...
  52. Gaur R, Dixit S, Sahoo M, Khanna M, Singh S, Murthy P. Anti-filarial activity of novel formulations of albendazole against experimental brugian filariasis. Parasitology. 2007;134:537-44 pubmed
    ..Six formulations (ALB-1 to ALB-6) were prepared and tested against Brugia malayi in Mastomys coucha and jird (Meriones unguiculatus) at 200 mg/kg, orally, for 5 consecutive days...
  53. Whitton C, Daub J, Quail M, Hall N, Foster J, Ware J, et al. A genome sequence survey of the filarial nematode Brugia malayi: repeats, gene discovery, and comparative genomics. Mol Biochem Parasitol. 2004;137:215-27 pubmed
    ..The Brugia malayi genome is approximately 100 Mb in size, and distributed across five chromosome pairs...
  54. Hise A, Daehnel K, Gillette Ferguson I, Cho E, McGarry H, Taylor M, et al. Innate immune responses to endosymbiotic Wolbachia bacteria in Brugia malayi and Onchocerca volvulus are dependent on TLR2, TLR6, MyD88, and Mal, but not TLR4, TRIF, or TRAM. J Immunol. 2007;178:1068-76 pubmed
    ..Extracts of Brugia malayi and Onchocerca volvulus, which contain Wolbachia, directly stimulated human embryonic kidney cells expressing ..
  55. Shenoy R, Suma T, Kumaraswami V, Dhananjayan G, Rahmah N, Abhilash G, et al. Lymphoscintigraphic evidence of lymph vessel dilation in the limbs of children with Brugia malayi infection. J Commun Dis. 2008;40:91-100 pubmed
    ..report here for the first time, the lymphatic abnormalities seen on lymphoscintigraphy (LSG) in children with Brugia malayi filariasis...
  56. Williams S, Lizotte Waniewski M, Foster J, Guiliano D, Daub J, Scott A, et al. The filarial genome project: analysis of the nuclear, mitochondrial and endosymbiont genomes of Brugia malayi. Int J Parasitol. 2000;30:411-9 pubmed
    The Filarial Genome Project (FGP) was initiated in 1994 under the auspices of the World Health Organisation. Brugia malayi was chosen as the model organism due to the availability of all life cycle stages for the construction of cDNA ..
  57. Vasuki V, Patra K, Hoti S. A rapid and simplified method of DNA extraction for the detection of Brugia malayi infection in mosquitoes by PCR assay. Acta Trop. 2001;79:245-8 pubmed
    ..methods (A, B, C and D) were tried for the extraction of DNA from mosquitoes infected with filarial parasite, Brugia malayi. Method D was found to be as efficient as the current procedure for the extraction of DNA from a single ..
  58. Murray J, Gregory W, Gomez Escobar N, Atmadja A, Maizels R. Expression and immune recognition of Brugia malayi VAL-1, a homologue of vespid venom allergens and Ancylostoma secreted proteins. Mol Biochem Parasitol. 2001;118:89-96 pubmed
    ..We isolated the corresponding gene from the human filarial nematode, Brugia malayi, by first searching the expressed sequence tag (EST) dataset generated by the Filarial Genome Project and then ..
  59. Aboobaker A, Blaxter M. Use of RNA interference to investigate gene function in the human filarial nematode parasite Brugia malayi. Mol Biochem Parasitol. 2003;129:41-51 pubmed
    ..genetic technique RNA interference (RNAi) to investigate gene function in the human filarial nematode parasite Brugia malayi. We used fluorescently labelled double stranded RNA (dsRNA) to demonstrate that 300 bp molecules are able to ..
  60. Rajan T, Paciorkowski N, Kalajzic I, McGuiness C. Ascorbic acid is a requirement for the morphogenesis of the human filarial parasite Brugia malayi. J Parasitol. 2003;89:868-70 pubmed
    The nematode parasites Wuchereria bancrofti, Brugia malayi, and B. timori cause a disease in humans known as lymphatic filariasis, which afflicts approximately 120 million people worldwide...
  61. Saint André A, Blackwell N, Hall L, Hoerauf A, Brattig N, Volkmann L, et al. The role of endosymbiotic Wolbachia bacteria in the pathogenesis of river blindness. Science. 2002;295:1892-5 pubmed
    ..In addition, the inflammatory response induced by these bacteria was dependent on expression of functional Toll-like receptor 4 (TLR4) on host cells...
  62. Semnani R, Law M, Kubofcik J, Nutman T. Filaria-induced immune evasion: suppression by the infective stage of Brugia malayi at the earliest host-parasite interface. J Immunol. 2004;172:6229-38 pubmed
    To assess the physiologic interactions between the infective stage of Brugia malayi--one of the extracellular parasites responsible for lymphatic filariasis in humans--and the APC with which they come in contact during their development ..
  63. Jiang D, Li B, Fischer P, Weil G. Localization of gender-regulated gene expression in the filarial nematode Brugia malayi. Int J Parasitol. 2008;38:503-12 pubmed
    We used in situ hybridization (ISH) to localize expression of gender-biased genes in the filarial parasite Brugia malayi that were previously identified by microarray analysis and quantitative reverse transcriptase PCR (qRT-PCR)...
  64. Murthy P, Dixit S, Gaur R, Kumar R, Sahoo M, Shakya N, et al. Influence of Brugia malayi life stages and BmAFII fraction on experimental Leishmania donovani infection in hamsters. Acta Trop. 2008;106:81-9 pubmed publisher
    The influence of live Brugia malayi parasites and a Sephadex G-200 fraction of the adult parasite extract (BmAFII) on the progression of Leishmania donovani infection was studied. Inbred hamsters were first infected with B...
  65. Gnanasekar M, Anandharaman V, Anand S, Nutman T, Ramaswamy K. A novel small heat shock protein 12.6 (HSP12.6) from Brugia malayi functions as a human IL-10 receptor binding protein. Mol Biochem Parasitol. 2008;159:98-103 pubmed publisher
    Phage display cDNA expression library of the third stage larvae (L3) of Brugia malayi was screened for identifying target(s) that bound to the human interleukin-10 receptor (huIL10R)...
  66. Veerapathran A, Dakshinamoorthy G, Gnanasekar M, Reddy M, Kalyanasundaram R. Evaluation of Wuchereria bancrofti GST as a vaccine candidate for lymphatic filariasis. PLoS Negl Trop Dis. 2009;3:e457 pubmed publisher
    ..However, vaccine potential of GSTs of lymphatic filarial parasites has not been evaluated before...
  67. Semnani R, Mahapatra L, Moore V, Sanprasert V, Nutman T. Functional and phenotypic characteristics of alternative activation induced in human monocytes by interleukin-4 or the parasitic nematode Brugia malayi. Infect Immun. 2011;79:3957-65 pubmed publisher
    ..parasite antigen in differentiation of human monocytes, cells were exposed to microfilariae (mf) of Brugia malayi, and their phenotypic and functional characteristics were compared with those of monocytes exposed to factors ..
  68. Foster J, Kumar S, Ganatra M, Kamal I, Ware J, Ingram J, et al. Construction of bacterial artificial chromosome libraries from the parasitic nematode Brugia malayi and physical mapping of the genome of its Wolbachia endosymbiont. Int J Parasitol. 2004;34:733-46 pubmed
    The parasitic nematode, Brugia malayi, causes lymphatic filariasis in humans, which in severe cases leads to the condition known as elephantiasis...
  69. Pokharel D, Rai R, Nandakumar Kodumudi K, Reddy M, Rathaur S. Vaccination with Setaria cervi 175 kDa collagenase induces high level of protection against Brugia malayi infection in jirds. Vaccine. 2006;24:6208-15 pubmed
    ..An adult 175 kDa collagenase antigen of Setaria cervi in immunoprophylaxis against Brugia malayi. J Helminth 2004;78:347-52]...
  70. Dangi A, Vedi S, Nag J, Paithankar S, Singh M, Kar S, et al. Tetracycline treatment targeting Wolbachia affects expression of an array of proteins in Brugia malayi parasite. Proteomics. 2009;9:4192-208 pubmed publisher
    Wolbachia is an intracellular endosymbiont of Brugia malayi parasite whose presence is essential for the survival of the parasite. Treatment of B...
  71. Ghedin E, Hailemariam T, DePasse J, Zhang X, Oksov Y, Unnasch T, et al. Brugia malayi gene expression in response to the targeting of the Wolbachia endosymbiont by tetracycline treatment. PLoS Negl Trop Dis. 2009;3:e525 pubmed publisher
    b>Brugia malayi, like most human filarial parasite species, harbors an endosymbiotic bacterium of the genus Wolbachia. Elimination of the endosymbiont leads to sterilization of the adult female...
  72. Bennuru S, Meng Z, Ribeiro J, Semnani R, Ghedin E, Chan K, et al. Stage-specific proteomic expression patterns of the human filarial parasite Brugia malayi and its endosymbiont Wolbachia. Proc Natl Acad Sci U S A. 2011;108:9649-54 pubmed publisher
    ..We report here a large-scale proteomic characterization of almost all the major mammalian stages of Brugia malayi, a causative agent of lymphatic filariasis, resulting in the identification of more than 62% of the products ..
  73. Cross H, Haarbrink M, Egerton G, Yazdanbakhsh M, Taylor M. Severe reactions to filarial chemotherapy and release of Wolbachia endosymbionts into blood. Lancet. 2001;358:1873-5 pubmed
    ..We took blood samples from 15 Indonesian patients before and after treatment with diethylcarbamazine for Brugia malayi infection, and recorded the severity of any post-treatment inflammatory reactions...
  74. Rathaur S, Yadav M, Gupta S, Anandharaman V, Reddy M. Filarial glutathione-S-transferase: a potential vaccine candidate against lymphatic filariasis. Vaccine. 2008;26:4094-100 pubmed publisher
    ..75% (p<0.005) reduction in adult parasite burden was observed in ScGST immunized group after 90 days post Brugia malayi L3 challenge...
  75. Holman A, Davis P, Foster J, Carlow C, Kumar S. Computational prediction of essential genes in an unculturable endosymbiotic bacterium, Wolbachia of Brugia malayi. BMC Microbiol. 2009;9:243 pubmed publisher
    Wolbachia (wBm) is an obligate endosymbiotic bacterium of Brugia malayi, a parasitic filarial nematode of humans and one of the causative agents of lymphatic filariasis...
  76. Tompkins J, Stitt L, Morrissette A, Ardelli B. The role of Brugia malayi ATP-binding cassette (ABC) transporters in potentiating drug sensitivity. Parasitol Res. 2011;109:1311-22 pubmed publisher
    ..The relative expression of 33 ABC systems identified in Brugia malayi was quantified following exogenous exposure to the commonly used drug ivermectin (IVM)...
  77. Hoti S, Vasuki V, Lizotte M, Patra K, Ravi G, Vanamail P, et al. Detection of Brugia malayi in laboratory and wild-caught Mansonioides mosquitoes (Diptera: Culicidae) using Hha I PCR assay. Bull Entomol Res. 2001;91:87-92 pubmed
    An Hha 1 based polymerase chain reaction (PCR) assay developed for the detection of Brugia malayi, the causative agent of Brugian lymphatic filariasis, was evaluated for its sensitivity in the laboratory and for its usefulness in ..
  78. Rahmah N, Lim B, Khairul Anuar A, Shenoy R, Kumaraswami V, Lokman Hakim S, et al. A recombinant antigen-based IgG4 ELISA for the specific and sensitive detection of Brugia malayi infection. Trans R Soc Trop Med Hyg. 2001;95:280-4 pubmed
    An IgG4 ELISA based on a novel recombinant antigen was evaluated for detection of Brugia malayi infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276 blinded sera from 2 other institutions ..
  79. Lakshmi V, Saxena A, Pandey K, Bajpai P, Misra Bhattacharya S. Antifilarial activity of Zoanthus species (Phylum Coelenterata, Class Anthzoa) against human lymphatic filaria, Brugia malayi. Parasitol Res. 2004;93:268-73 pubmed
    ..Class Anthozoa) showed promising in vitro adulticidal activity with a lethal concentration of 125 microg/ml on Brugia malayi. This extract brought about a 52.2% reduction in circulating microfilariae of B...
  80. Ardelli B, Stitt L, Tompkins J. Inventory and analysis of ATP-binding cassette (ABC) systems in Brugia malayi. Parasitology. 2010;137:1195-212 pubmed publisher
    ..Mining and PCR-based cloning were used to identify 33 putative ABC systems from the Brugia malayi genome. There were 31 class 2 genes, commonly called ABC transporters, and 2 class 3 genes...
  81. Landmann F, Foster J, Slatko B, Sullivan W. Efficient in vitro RNA interference and immunofluorescence-based phenotype analysis in a human parasitic nematode, Brugia malayi. Parasit Vectors. 2012;5:16 pubmed publisher
    ..immunostaining techniques yield detailed analysis of cytological defects in the human parasitic nematode, Brugia malayi. The cellular disorganization observed in B...
  82. Stanley P, Stein P. BmSPN2, a serpin secreted by the filarial nematode Brugia malayi, does not inhibit human neutrophil proteinases but plays a noninhibitory role. Biochemistry. 2003;42:6241-8 pubmed
    The filarial nematode, Brugia malayi, is a causative agent of lymphatic filariasis. Bm-spn-2, one of two serpin genes identified in B...
  83. Thanchomnang T, Intapan P, Lulitanond V, Choochote W, Manjai A, Prasongdee T, et al. Rapid detection of Brugia malayi in mosquito vectors using a real-time fluorescence resonance energy transfer PCR and melting curve analysis. Am J Trop Med Hyg. 2008;78:509-13 pubmed
    ..energy transfer (FRET) polymerase chain reaction (PCR) combined with melting curve analysis for detection of Brugia malayi DNA in blood-fed mosquitoes...
  84. Moreno Y, Nabhan J, Solomon J, Mackenzie C, Geary T. Ivermectin disrupts the function of the excretory-secretory apparatus in microfilariae of Brugia malayi. Proc Natl Acad Sci U S A. 2010;107:20120-5 pubmed publisher
    ..We localized Brugia malayi GluCl expression solely in a muscle structure that surrounds the microfilarial excretory-secretory (ES) ..
  85. Babayan S, Allen J, Taylor D. Future prospects and challenges of vaccines against filariasis. Parasite Immunol. 2012;34:243-53 pubmed publisher
    ..onchocerciasis (river blindness, Onchocerca volvulus) and lymphatic filariasis (Wuchereria bancrofti or Brugia malayi) is founded on evidence from both humans and animal models for the development of protective immunity...
  86. Rao R, Well G. In vitro effects of antibiotics on Brugia malayi worm survival and reproduction. J Parasitol. 2002;88:605-11 pubmed
    ..The effects of antibiotics that are active against related bacteria on adult worms and microfilariae (Mf) of Brugia malayi in vitro were investigated...
  87. Fischer P, Wibowo H, Pischke S, Ruckert P, Liebau E, Ismid I, et al. PCR-based detection and identification of the filarial parasite Brugia timori from Alor Island, Indonesia. Ann Trop Med Parasitol. 2002;96:809-21 pubmed
    ..malayi by other molecular markers. PCR-based techniques targeting the HhaI repeat can therefore be employed for monitoring B. timori in the framework of the Global Programme to Eliminate Lymphatic Filariasis...
  88. Fischer P, Bonow I, Supali T, Ruckert P, Rahmah N. Detection of filaria-specific IgG4 antibodies and filarial DNA, for the screening of blood spots for Brugia timori. Ann Trop Med Parasitol. 2005;99:53-60 pubmed
    ..an immunochromatographic dipstick is used to detect IgG(4) antibodies that are reactive with a recombinant Brugia malayi antigen. When sera from 109 individuals with Brugia microfilaraemias (12 with B. malayi and 97 with B...
  89. Moreno Y, Geary T. Stage- and gender-specific proteomic analysis of Brugia malayi excretory-secretory products. PLoS Negl Trop Dis. 2008;2:e326 pubmed publisher
    ..the Excretory-Secretory Products (ESP) of adult female, adult male and microfilariae of the filarial nematode Brugia malayi, one of the etiological agents of human lymphatic filariasis...
  90. Maizels R, Blaxter M, Scott A. Immunological genomics of Brugia malayi: filarial genes implicated in immune evasion and protective immunity. Parasite Immunol. 2001;23:327-44 pubmed
    ..These gene products provide a fascinating glimpse of the molecular repertoire which helminth parasites have evolved to manipulate and evade the mammalian immune response. ..
  91. Foster J, Zhang Y, Kumar S, Carlow C. Parasitic nematodes have two distinct chitin synthases. Mol Biochem Parasitol. 2005;142:126-32 pubmed
  92. Singh M, Srivastava K, Bhattacharya S. Molecular cloning and characterization of a novel immunoreactive ATPase/RNA helicase in human filarial parasite Brugia malayi. Parasitol Res. 2009;104:753-61 pubmed publisher
    ..cDNA clone, BmL3-helicase, encoding DEAD box RNA helicase by immunoscreening of a larval stage cDNA library of Brugia malayi. The cDNA sequence exhibited strong sequence homology to Caenorhabditis elegans and C...