Summary: A genus of bioluminescent marine invertebrates in the family Renillidae, order Pennatulacea, class ANTHOZOA. It contains Renilla LUCIFERASE which oxidizes coelenterazine resulting in LUMINESCENCE.

Top Publications

  1. Ghazawi I, Cutler S, Low P, Mellick A, Ralph S. Inhibitory effects associated with use of modified Photinus pyralis and Renilla reniformis luciferase vectors in dual reporter assays and implications for analysis of ISGs. J Interferon Cytokine Res. 2005;25:92-102 pubmed
    ..We report that the recently developed codon-modified Renilla luciferase construct included as an internal standard for cotransfection must be used with great caution with ..
  2. Burbelo P, Goldman R, Mattson T. A simplified immunoprecipitation method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced Renilla luciferase-antigen fusion proteins. BMC Biotechnol. 2005;5:22 pubmed
  3. Venisnik K, Olafsen T, Loening A, Iyer M, Gambhir S, Wu A. Bifunctional antibody-Renilla luciferase fusion protein for in vivo optical detection of tumors. Protein Eng Des Sel. 2006;19:453-60 pubmed
    ..antigen (CEA) antibody fragment, the anti-CEA diabody, was fused to the bioluminescence enzyme Renilla luciferase (RLuc) to generate a novel optical imaging probe...
  4. Loening A, Fenn T, Gambhir S. Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis. J Mol Biol. 2007;374:1017-28 pubmed
    Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely employed in molecular biology as a reporter gene in cell culture experiments and small animal imaging...
  5. van den Pol A, Yao Y, Fu L, Foo K, Huang H, Coppari R, et al. Neuromedin B and gastrin-releasing peptide excite arcuate nucleus neuropeptide Y neurons in a novel transgenic mouse expressing strong Renilla green fluorescent protein in NPY neurons. J Neurosci. 2009;29:4622-39 pubmed publisher
    ..Transgenic mice were generated that expressed bright Renilla green fluorescent protein (GFP) in most or all of the known NPY cells in the brain, which otherwise were not ..
  6. Jiang Y, Bernard D, Yu Y, Xie Y, Zhang T, Li Y, et al. Split Renilla luciferase protein fragment-assisted complementation (SRL-PFAC) to characterize Hsp90-Cdc37 complex and identify critical residues in protein/protein interactions. J Biol Chem. 2010;285:21023-36 pubmed publisher
    ..The purpose of this study was to utilize split Renilla luciferase protein fragment-assisted complementation (SRL-PFAC) bioluminescence to study the full-length human ..
  7. Kimura T, Hiraoka K, Kasahara N, Logg C. Optimization of enzyme-substrate pairing for bioluminescence imaging of gene transfer using Renilla and Gaussia luciferases. J Gene Med. 2010;12:528-37 pubmed publisher
    ..Improvements in light output from bioluminescent reporter systems would allow the detection of lower levels of expression, smaller numbers of cells and expression from deeper and more attenuating tissues within an animal...
  8. Estephane D, Anctil M. Retinoic acid and nitric oxide promote cell proliferation and differentially induce neuronal differentiation in vitro in the cnidarian Renilla koellikeri. Dev Neurobiol. 2010;70:842-52 pubmed publisher
    ..We used primary cultures of cells from the sea pansy Renilla koellikeri to investigate the involvement of these signaling molecules in cnidarian cell differentiation...
  9. Hatzios S, Ringgaard S, Davis B, Waldor M. Studies of dynamic protein-protein interactions in bacteria using Renilla luciferase complementation are undermined by nonspecific enzyme inhibition. PLoS ONE. 2012;7:e43175 pubmed publisher
    ..As proof of concept and to develop a new tool for studies of bacterial chemotaxis, fragments of Renilla luciferase (Rluc) were fused to the chemotaxis-associated response regulator CheY3 and its phosphatase CheZ in ..

More Information

Publications139 found, 100 shown here

  1. Tannous B, Kim D, Fernandez J, Weissleder R, Breakefield X. Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol Ther. 2005;11:435-43 pubmed
    ..alone (not including secreted luciferase) or cell lysates, compared to humanized forms of firefly (hFLuc) and Renilla (hRLuc) luciferases expressed under similar conditions...
  2. Hampf M, Gossen M. A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays. Anal Biochem. 2006;356:94-9 pubmed
    ..quantitative reporter gene protocol, the P/Rluc assay system, allowing the sequential measurement of Photinus and Renilla luciferase activities from the same extract...
  3. Loening A, Fenn T, Wu A, Gambhir S. Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output. Protein Eng Des Sel. 2006;19:391-400 pubmed
    ..activity of commonly used luciferases is too labile in serum to permit this application, specific mutations of Renilla luciferase, selected using a consensus sequence driven strategy, were screened for their ability to confer ..
  4. Loening A, Wu A, Gambhir S. Red-shifted Renilla reniformis luciferase variants for imaging in living subjects. Nat Methods. 2007;4:641-3 pubmed
    ..To overcome this, we generated variants of RLuc with bathochromic (red) shifts of up to 66 nm (547 nm peak) that also had greater stability and higher light emission than native RLuc...
  5. Liu S, Nelson C, Xiao L, Lu L, Seth P, Davis D, et al. Measuring antiviral activity of benzimidazole molecules that alter IRES RNA structure with an infectious hepatitis C virus chimera expressing Renilla luciferase. Antiviral Res. 2011;89:54-63 pubmed publisher
    ..Moreover, the V3 region can be replaced with the Renilla reniformis luciferase (Rluc) gene resulting in an infectious virus that stably expresses an NS5A-Rluc fusion ..
  6. Andou T, Endoh T, Mie M, Kobatake E. Direct detection of RNAs in living cells using peptide-inserted Renilla luciferase. Analyst. 2011;136:2446-9 pubmed publisher
    ..Two types of probe were utilized: a peptide inserted RLuc (PI-RLuc) probe and a split-RNA probe. Incorporation of the PI-RLuc and split-RNA probes enabled the direct detection of RNA introduced into living cells...
  7. Banerjee S, Yang S, Foster C. A luciferase reporter assay to investigate the differential selenium-dependent stability of selenoprotein mRNAs. J Nutr Biochem. 2012;23:1294-301 pubmed publisher
    ..Our reporter is a fusion of the Renilla luciferase gene and of the ?-globin gene, but contains features required for incorporation of selenocysteine (SEC)..
  8. Eguchi T, Kubota S, Takigawa M. Promoter Analyses of CCN Genes. Methods Mol Biol. 2017;1489:177-185 pubmed
    ..Luciferase is an enzyme that is used when firefly and Renilla reniformis (sea pansy) emit light...
  9. Li J, Deng C, Gu D, Li X, Shi L, He J, et al. Development of a replicon cell line-based high throughput antiviral assay for screening inhibitors of Zika virus. Antiviral Res. 2018;150:148-154 pubmed publisher
    ..Here, we constructed a dicistronic ZIKV replicon (ZIKV-Pac-Rluc-Rep) that contained the Renilla luciferase (Rluc) reporter gene separated from the puromycin N-acetyl-transferase (Pac) selectable marker by a ..
  10. Coutant E, Janin Y. Synthetic Routes to Coelenterazine and Other Imidazo[1,2-a]pyrazin-3-one Luciferins: Essential Tools for Bioluminescence-Based Investigations. Chemistry. 2015;21:17158-71 pubmed publisher
    ..of imidazo[1,2-a]pyrazine luciferins along with photoproteins or luciferases from sea creatures such as Aequorea, Renilla, Gaussia or Oplophorus...
  11. Terrazas M, Ivani I, Villegas N, Paris C, Salvans C, Brun Heath I, et al. Rational design of novel N-alkyl-N capped biostable RNA nanostructures for efficient long-term inhibition of gene expression. Nucleic Acids Res. 2016;44:4354-67 pubmed publisher
    ..alkyl chain and stem lengths on RNAi activity in a screen involving two series of dumbbell analogues targeting Renilla and Firefly luciferase genes...
  12. Chiba S, Jamal A, Suzuki N. First Evidence for Internal Ribosomal Entry Sites in Diverse Fungal Virus Genomes. MBio. 2018;9: pubmed publisher
    ..A bicistronic construct entails a codon-optimized Renilla and firefly luciferase (ORluc and OFluc, respectively) gene, between which potential IRES ..
  13. Rahnama S, Saffar B, Kahrani Z, Nazari M, Emamzadeh R. Super RLuc8: A novel engineered Renilla luciferase with a red-shifted spectrum and stable light emission. Enzyme Microb Technol. 2017;96:60-66 pubmed publisher
    b>Renilla luciferase is a bioluminescent enzyme which is broadly used as a reporter protein in molecular biosensors. In this study, a novel luciferase with desired light emission wavelength and thermostability is reported...
  14. Yang X, Li Z, Jiang T, Du L, Li M. A coelenterazine-type bioluminescent probe for nitroreductase imaging. Org Biomol Chem. 2017;16:146-151 pubmed publisher
    ..resulting in 1,4 or 1,6-rearrangement-elimination to release coelenterazine analogues, which can be catalyzed by Renilla luciferase to emit bioluminescence...
  15. Kang B, Jeon H, Chae Y, Lee S, Park J, Choi G, et al. Impact of Genetic Variation in MicroRNA-binding Site on Susceptibility to Colorectal Cancer. Anticancer Res. 2016;36:3353-61 pubmed
    ..84, 95% CI=0.73-0.96, p=0.012; aOR=0.65, CI=0.49-0.88, p=0.004, respectively). Significantly lower Renilla activity was also observed with the rs2279398 AA construct when compared to the rs2279398 GG construct (p<0...
  16. Inouye S, Sahara Miura Y, Sato J, Iimori R, Yoshida S, Hosoya T. Expression, purification and luminescence properties of coelenterazine-utilizing luciferases from Renilla, Oplophorus and Gaussia: comparison of substrate specificity for C2-modified coelenterazines. Protein Expr Purif. 2013;88:150-6 pubmed publisher
    ..coli is useful and we have applied this system to prepare the coelenterazine-utilizing luciferases including Renilla luciferase (RLase), a red-shifted variant of Renilla luciferase (RLase-547), the catalytic domain of Oplophorus ..
  17. Gao F, Qu Z, Li L, Yu L, Jiang Y, Zhou Y, et al. Recombinant porcine reproductive and respiratory syndrome virus expressing luciferase genes provide a new indication of viral propagation in both permissive and target cells. Res Vet Sci. 2016;107:132-140 pubmed publisher
    ..genetic system of type 2 highly pathogenic PRRSV cell-passage attenuated strain pHuN4-F112, firefly luciferase or Renilla luciferase genes were inserted between ORF1b and ORF2...
  18. Kumar M, Kovalski L, Broyles D, Hunt E, Daftarian P, Dikici E, et al. Design and development of high bioluminescent resonance energy transfer efficiency hybrid-imaging constructs. Anal Biochem. 2016;498:1-7 pubmed publisher
    ..composed of multiple quantum dots (QDs; λem = 655 nm) self-assembled onto a bioluminescent protein, Renilla luciferase (Rluc)...
  19. Wang J, Guo W, Long C, Zhou H, Wang H, Sun X. The split Renilla luciferase complementation assay is useful for identifying the interaction of Epstein-Barr virus protein kinase BGLF4 and a heat shock protein Hsp90. Acta Virol. 2016;60:62-70 pubmed
    ..The split Renilla luciferase complementation assay (SRLCA) is one of the techniques that detect protein-protein interactions...
  20. Woo J, Hong J, Dinesh Kumar S. Bioluminescence Resonance Energy Transfer (BRET)-Based Synthetic Sensor Platform for Drug Discovery. Curr Protoc Protein Sci. 2017;88:19.30.1-19.30.12 pubmed publisher
    ..The unique feature of BRET delineates that the resonance energy is generated by the resonance energy donor, Renilla luciferase by the oxidative decarboxylation of coelenterazine substrate...
  21. Gurjar S, Derrick J, Dearman R, Thorpe R, Hufton S, Kimber I, et al. Surrogate CD16-expressing effector cell lines for determining the bioactivity of therapeutic monoclonal antibodies. J Pharm Biomed Anal. 2017;143:188-198 pubmed publisher
    ..However, use of the J9 cells containing the constitutively expressed renilla luciferase gene enabled data normalisation and corrected for fluctuations in both cell number and viability ..
  22. Wang D, Sang H, Zhang K, Nie Y, Zhao S, Zhang Y, et al. Stat3 phosphorylation is required for embryonic stem cells ground state maintenance in 2i culture media. Oncotarget. 2017;8:31227-31237 pubmed publisher
    ..We introduced a lentiviral construct with 7-repeat Stat3-binding sequence to drive Renilla luciferase into ES cells, which can be used as a reporter to detect Stat3 activation by noninvasive ..
  23. Ning S, Sekar T, Scicinski J, Oronsky B, Peehl D, Knox S, et al. Nrf2 activity as a potential biomarker for the pan-epigenetic anticancer agent, RRx-001. Oncotarget. 2015;6:21547-56 pubmed
    ..Molecular imaging of tumor cells co-expressing pARE-Firefly luciferase and pCMV-Renilla luciferase-mRFP in vitro and in vivo in mice revealed that RRx-001 significantly increased ARE-FLUC signal in ..
  24. Leng W, Li D, Chen L, Xia H, Tang Q, Chen B, et al. Novel Bioluminescent Activatable Reporter for Src Tyrosine Kinase Activity in Living Mice. Theranostics. 2016;6:594-609 pubmed publisher
    ..Using both Firefly and Renilla luciferase imaging, we have successfully monitored Src tyrosine kinase activity and Akt serine/threonine kinase ..
  25. Bouchard C, Ribeiro P, Dubé F, Demers C, Anctil M. Identification of a novel aminergic-like G protein-coupled receptor in the cnidarian Renilla koellikeri. Gene. 2004;341:67-75 pubmed
    ..We have cloned a novel G protein-coupled receptor cDNA from an anthozoan, the sea pansy Renilla koellikeri, that shows homology to mammalian catecholamine receptors and, to a lesser extent, to peptidergic ..
  26. Askarian S, Abnous K, Darroudi M, Oskuee R, Ramezani M. Gene delivery to neuroblastoma cells by poly (l-lysine)-grafted low molecular weight polyethylenimine copolymers. Biologicals. 2016;44:212-218 pubmed publisher
    ..b>Renilla luciferase assay was employed to evaluate the gene transfection efficiency of pDNA-polymer to Neuro2A cell line...
  27. Shen L, Yang Y, Ye F, Liu G, Desforges M, Talbot P, et al. Safe and Sensitive Antiviral Screening Platform Based on Recombinant Human Coronavirus OC43 Expressing the Luciferase Reporter Gene. Antimicrob Agents Chemother. 2016;60:5492-503 pubmed publisher
    ..Here, we developed four infectious recombinant HCoVs-OC43 (rHCoVs-OC43) which express the Renilla luciferase (Rluc) reporter gene...
  28. Kulkarni V, Naqvi A, Uttamani J, Nares S. MiRNA-Target Interaction Reveals Cell-Specific Post-Transcriptional Regulation in Mammalian Cell Lines. Int J Mol Sci. 2016;17: pubmed publisher
    ..mechanistic differences in the regulation of a let-7a targeted (wild type) or resistant (mutant) engineered renilla transcript across various mammalian cell lines of diverse origin...
  29. Shoji M, Arakaki Y, Esumi T, Kohnomi S, Yamamoto C, Suzuki Y, et al. Bakuchiol Is a Phenolic Isoprenoid with Novel Enantiomer-selective Anti-influenza A Virus Activity Involving Nrf2 Activation. J Biol Chem. 2015;290:28001-17 pubmed publisher
    ..assay data unexpectedly indicated that bakuchiol may induce some host cell factor(s) that inhibited firefly and Renilla luciferases...
  30. Bodas M, Mazur S, Min T, Vij N. Inhibition of histone-deacetylase activity rescues inflammatory cystic fibrosis lung disease by modulating innate and adaptive immune responses. Respir Res. 2018;19:2 pubmed publisher
    ..For in vitro experiments, HEK293-cells were transfected with IL-8 or NFκB-firefly luciferase, and SV40-renilla- luciferase reporter constructs or ΔF508-CFTR-pCEP, followed by treatment with suberoylanilide hydroxamic acid (..
  31. Marcos Vadillo E, García Sánchez A. Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line. Methods Mol Biol. 2016;1434:199-211 pubmed publisher
    ..this chapter, we show a transfection protocol of A549 lung epithelial cells with a reporter vector, using the Luciferase-Renilla dual system for studying the variations caused by several polymorphisms in the promoter region of a gene.
  32. Farkas T, Jaattela M. Renilla Luciferase-LC3 Based Reporter Assay for Measuring Autophagic Flux. Methods Enzymol. 2017;588:1-13 pubmed publisher
    ..The Rluc-LC3 assay has proven useful for the identification of genes, miRNAs, and small molecules that regulate autophagy flux in mammalian cells. ..
  33. Jiang T, Yang X, Yang X, Yuan M, Zhang T, Zhang H, et al. Novel bioluminescent coelenterazine derivatives with imidazopyrazinone C-6 extended substitution for Renilla luciferase. Org Biomol Chem. 2016;14:5272-81 pubmed publisher
    ..After extensive evaluation, some compounds display excellent bioluminescence properties compared with DeepBlueC in cellulo, thus becoming potential molecules for bioluminescence techniques. ..
  34. Fanaei Kahrani Z, Emamzadeh R, Nazari M, Rasa S. Molecular basis of thermostability enhancement of Renilla luciferase at higher temperatures by insertion of a disulfide bridge into the structure. Biochim Biophys Acta Proteins Proteom. 2017;1865:252-259 pubmed publisher
    b>Renilla luciferase (RLuc), also known as Renilla-luciferin 2-monooxygenase, is a light producing enzyme used in many biotechnological applications such as bioreporters...
  35. Gardner C, Trobaugh D, Ryman K, Klimstra W. Electroporation of Alphavirus RNA Translational Reporters into Fibroblastic and Myeloid Cells as a Tool to Study the Innate Immune System. Methods Mol Biol. 2016;1428:127-37 pubmed publisher
    ..a method for electroporating viral RNA reporters into both fibroblastic and myeloid cells that encode firefly or Renilla luciferase, whose reaction with specific substrates and light emitting activity is a measure of viral RNA ..
  36. Kelwick R, Webb A, MacDonald J, Freemont P. Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements. Metab Eng. 2016;38:370-381 pubmed publisher
    ..Additionally, we demonstrate the cell-free characterisation of an inducible expression system, and the activity of a model enzyme - renilla luciferase.
  37. Kitagawa Y, Sakai M, Funayama M, Itoh M, Gotoh B. Human Metapneumovirus M2-2 Protein Acts as a Negative Regulator of Alpha Interferon Production by Plasmacytoid Dendritic Cells. J Virol. 2017;91: pubmed publisher
    ..Bioluminescence resonance energy transfer assays and split Renilla luciferase complementation assays revealed that M2-2 inhibited MyD88/TRAF6/IKKα-induced homodimerization ..
  38. Ishibashi M, Kawanabe R, Amaba N, Arai S, Laksmi F, Komori K, et al. Expression and characterization of the Renilla luciferase with the cumulative mutation. Protein Expr Purif. 2018;145:39-44 pubmed publisher
    Luciferase from Renilla reniformis (RLuc) is a good research tool as a reporter protein and bioimaging probes, yielding blue light using the substrate coelenterazine...
  39. Zhang Y, Robertson J, Xie Q, Johnson C. Monitoring Intracellular pH Change with a Genetically Encoded and Ratiometric Luminescence Sensor in Yeast and Mammalian Cells. Methods Mol Biol. 2016;1461:117-30 pubmed publisher
    ..pHlash is a fusion protein between a mutant of Renilla luciferase (RLuc) and a Venus fluorophore...
  40. Liyaghatdar Z, Emamzadeh R, Rasa S, Nazari M. Trehalose radial networks protect Renilla luciferase helical layers against thermal inactivation. Int J Biol Macromol. 2017;105:66-73 pubmed publisher
    b>Renilla luciferase (Rluc) from Renilla reniformis is an appropriate protein reporter for the detection of specific molecular targets due to its bioluminescent feature, although its relatively low stability limits the application...
  41. Szyska M, Herda S, Althoff S, Heimann A, Russ J, D Abundo D, et al. A Transgenic Dual-Luciferase Reporter Mouse for Longitudinal and Functional Monitoring of T Cells In Vivo. Cancer Immunol Res. 2018;6:110-120 pubmed publisher
    ..imaging of T cells (BLITC) mice expressing an NFAT-dependent click-beetle luciferase and a constitutive Renilla luciferase, which supports concomitant in vivo analysis of migration and activation of T cells...
  42. Varnum M, Clayton K, Yoshii Kitahara A, Yonemoto G, Koro L, Ikezu S, et al. A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells. J Biol Chem. 2017;292:10651-10663 pubmed publisher
    ..complementation technology based on TREM2 and TYROBP fusion to the C- or N-terminal portion of the Renilla luciferase gene...
  43. Shin Y, Choi B, Kim K, Kang C, Kim K, Yoon C. Development of a dual reporter screening assay for distinguishing the inhibition of HIV Tat-mediated transcription from off-target effects. J Virol Methods. 2017;249:1-9 pubmed publisher inducible system based on two lentiviral expression cassettes for doxycycline (Dox)-inducible Tat and Renilla luciferase (R-Luc) using TZM-bl cells harboring LTR-driven firefly luciferase (F-Luc)...
  44. Vengut Climent E, Terrazas M, Lucas R, Arévalo Ruiz M, Eritja R, Morales J. Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates. Bioorg Med Chem Lett. 2013;23:4048-51 pubmed publisher
    ..Such modifications contribute positively to the stability of oligoribonucleotides against 5'-exonuclease degradation. ..
  45. Patel N, Garikapati K, Ramaiah M, Polavarapu K, Bhadra U, Bhadra M. miR-15a/miR-16 induces mitochondrial dependent apoptosis in breast cancer cells by suppressing oncogene BMI1. Life Sci. 2016;164:60-70 pubmed publisher
    ..b>Renilla luciferase reporter assay was performed to detect the ectopically expressed miRNAs that regulate the expression ..
  46. Kim S, Jo H, Jeon M, Choi M, Hahn S, Yun S. Luciferase-Rose Bengal conjugates for singlet oxygen generation by bioluminescence resonance energy transfer. Chem Commun (Camb). 2017;53:4569-4572 pubmed publisher
    Conjugates of Rose Bengal and Renilla luciferase generated singlet oxygen upon binding with coelenterazine via bioluminescence resonance energy transfer (BRET)...
  47. Torres Quesada O, Rock R, Stefan E. Systematic Quantification of GPCR/cAMP-Controlled Protein Kinase A Interactions. Horm Metab Res. 2017;49:240-249 pubmed publisher
    ..of unimolecular and bimolecular PKA biosensors, followed by the specification of the modular design of a Renilla luciferase based protein-fragment complementation assay (PCA) platform for measuring PKA network interactions...
  48. Inan C, Muratoglu H, Arif B, Demirbag Z. Transcriptional analysis of the putative glycosyltransferase gene (amv248) of the Amsacta moorei entomopoxvirus. Virus Res. 2018;243:25-30 pubmed publisher
    ..The constructs were used to measure the Firefly and Renilla luciferase activities in dual assays...
  49. Yamamoto M, Matsuyama S, Li X, Takeda M, Kawaguchi Y, Inoue J, et al. Identification of Nafamostat as a Potent Inhibitor of Middle East Respiratory Syndrome Coronavirus S Protein-Mediated Membrane Fusion Using the Split-Protein-Based Cell-Cell Fusion Assay. Antimicrob Agents Chemother. 2016;60:6532-6539 pubmed publisher
    ..Here, we developed a cell-based fusion assay for S in a TMPRSS2-dependent manner using cell lines expressing Renilla luciferase (RL)-based split reporter proteins...
  50. Vazquez Vilar M, Quijano Rubio A, Fernandez del Carmen A, Sarrión Perdigones A, Ochoa Fernandez R, Ziarsolo P, et al. GB3.0: a platform for plant bio-design that connects functional DNA elements with associated biological data. Nucleic Acids Res. 2017;45:2196-2209 pubmed publisher
    ..We report the use of the Luciferase/Renilla (Luc/Ren) transient agroinfiltration assay in Nicotiana benthamiana as a standard to estimate relative ..
  51. Ward W. Biochemical and physical properties of green fluorescent protein. Methods Biochem Anal. 2006;47:39-65 pubmed
  52. Xie W, Silvers R, Ouellette M, Wu Z, Lu Q, Li H, et al. A Luciferase Reporter Gene System for High-Throughput Screening of γ-Globin Gene Activators. Methods Mol Biol. 2016;1439:207-26 pubmed publisher
    ..of the firefly luciferase gene, followed downstream by a synthetic β-globin gene promoter in front of the Renilla luciferase gene...
  53. Zhang D, Atlasi S, Patel K, Zhuang Z, Heaney A. False responses of Renilla luciferase reporter control to nuclear receptor TR4. Mol Cell Biochem. 2017;430:139-147 pubmed publisher
    b>Renilla luciferase reporter is a widely used internal control in dual luciferase reporter assay system, where its transcription is driven by a constitutively active promoter...
  54. Tin C, Yuan L, Dexter R, Parra G, Bui T, Green K, et al. A Luciferase Immunoprecipitation System (LIPS) assay for profiling human norovirus antibodies. J Virol Methods. 2017;248:116-129 pubmed publisher
    ..Recombinant proteins, expressed by plasmid constructs encoding Renilla luciferase (Ruc) fused to the full-length HuNoV major capsid protein (VP1) (Ruc-antigen), were generated for ten ..
  55. Lo M, Nichol S, Spiropoulou C. Evaluation of luciferase and GFP-expressing Nipah viruses for rapid quantitative antiviral screening. Antiviral Res. 2014;106:53-60 pubmed publisher
    ..We constructed and rescued NiVs expressing either Renilla luciferase or green fluorescent protein, and characterized their reporter signal kinetics in different cell types ..
  56. Ashkenazi S, Plotnikov A, Bahat A, Dikstein R. Effective cell-free drug screening protocol for protein-protein interaction. Anal Biochem. 2017;532:53-59 pubmed publisher
    ..We engineered a bacterial expression system for the split-Renilla luciferase (RL) complementation assay that monitors PPI...
  57. Xu F, Chai M, Li W, Ping Y, Tang G, Yang W, et al. Well-defined poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) vectors with low toxicity and high gene transfection efficiency. Biomacromolecules. 2010;11:1437-42 pubmed publisher
    ..A series of transfection and cytotoxicity assays revealed that PGEAs are highly promising as a new class of safe and efficient gene delivery vectors for future clinical gene therapies. ..
  58. Kubo T, Yanagihara K, Sato Y, Morita Y, Seyama T. Enhancement of gene silencing effect and membrane permeability by Peptide-conjugated 27-nucleotide small interfering RNA. Molecules. 2012;17:11089-102 pubmed publisher
    ..This potent RNAi efficacy is probably owing to acceleration of RISC through recognition by Dicer, as well as to the improvement of cell membrane permeability and intracellular accumulation. ..
  59. Lin J, Hisaoka M, Nagata K, Okuwaki M. Functional characterization and efficient detection of Nucleophosmin/NPM1 oligomers. Biochem Biophys Res Commun. 2016;480:702-708 pubmed publisher
    ..We utilized the split synthetic Renilla luciferase protein fragment-assisted complementation (SRL-PFAC) bioluminescence activity and observed the change ..
  60. Deshpande S, Masurkar N, Girish V, Desai M, Chakraborty G, Chan J, et al. Thermostable exoshells fold and stabilize recombinant proteins. Nat Commun. 2017;8:1442 pubmed publisher
    ..accommodation and charge complementation to green fluorescent protein (GFPuv), horseradish peroxidase (HRPc), and Renilla luciferase (rLuc), improving the yields of functional in vitro folding by ~100-fold...
  61. Ma N, Marshall A, Rao J. Near-infrared light emitting luciferase via biomineralization. J Am Chem Soc. 2010;132:6884-5 pubmed publisher
    ..This is the first study to form dual functional bioinorganic hybrid nanostructures via active enzyme-templated synthesis of inorganic nanomaterials. ..
  62. Samantarrai D, Mallick B. miR-429 inhibits metastasis by targeting KIAA0101 in Soft Tissue Sarcoma. Exp Cell Res. 2017;357:33-39 pubmed publisher
    ..Moreover, the renilla luciferase reporter assay confirmed that miR-429 targets KIAA0101 by binding to its 3/UTR and ..
  63. Qiu L, Chen J, Lin J, Wo D, Chu J, Peng J. Baicalin alleviates H2O2?induced injury of H9c2 cardiomyocytes through suppression of the Wnt/??catenin signaling pathway. Mol Med Rep. 2017;16:9251-9255 pubmed publisher
    ..The results of the TOPFlash/Renilla reporter gene assay indicated that baicalin significantly suppressed the activation of proto?oncogene Wnt?1 (Wnt)/..
  64. Matz M, Labas Y, Ugalde J. Evolution of function and color in GFP-like proteins. Methods Biochem Anal. 2006;47:139-61 pubmed
  65. Rani C, Elango N, Wang S, Kobayashi K, Strong R. Identification of an activator protein-1-like sequence as the glucocorticoid response element in the rat tyrosine hydroxylase gene. Mol Pharmacol. 2009;75:589-98 pubmed publisher
    ..Our studies describe for the first time a novel GRE/AP-1 site present in the TH gene promoter that is critical for glucocorticoid regulation of the TH gene. ..
  66. Martín Sánchez F, Compan V, Pelegrin P. Measuring NLR Oligomerization III: Detection of NLRP3 Complex by Bioluminescence Resonance Energy Transfer. Methods Mol Biol. 2016;1417:159-68 pubmed publisher
    ..BRET) is a natural phenomenon resulting from a non-radiative energy transfer between a bioluminescent donor (Renilla luciferase) and a fluorescent protein acceptor...
  67. Balasubramanian A, Manzano M, Teramoto T, Pilankatta R, Padmanabhan R. High-throughput screening for the identification of small-molecule inhibitors of the flaviviral protease. Antiviral Res. 2016;134:6-16 pubmed publisher
    ..been extensively analyzed for inhibition of the viral protease in vitro and cell-based viral replication using Renilla luciferase reporter replicon, infectivity (plaque) and cytotoxicity assays...
  68. Connick J, Reed J, Backes W. Characterization of Interactions Among CYP1A2, CYP2B4, and NADPH-cytochrome P450 Reductase: Identification of Specific Protein Complexes. Drug Metab Dispos. 2018;46:197-203 pubmed publisher
    ..Various pairs of P450 and POR constructs were tagged with either green fluorescent protein or Renilla luciferase, and transfected into human embryonic kidney 293T cells...
  69. Hall M, Unch J, Binkowski B, Valley M, Butler B, Wood M, et al. Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem Biol. 2012;7:1848-57 pubmed publisher
    ..half-life >2 h) with a specific activity ~150-fold greater than that of either firefly (Photinus pyralis) or Renilla luciferases similarly configured for glow-type assays...
  70. Song W, Sung H, Park K, Choi J, Cho J, Um S. Novel functional Renilla luciferase mutant provides long-term serum stability and high luminescence activity. Protein Expr Purif. 2013;91:215-20 pubmed publisher
    ..For the preparation of the modified Renilla luciferase, a new bacterial subcloning design was established...
  71. Zhang H, Bai H, Jiang T, Ma Z, Cheng Y, Zhou Y, et al. Quenching the firefly bioluminescence by various ions. Photochem Photobiol Sci. 2016;15:244-9 pubmed publisher
    ..some specific ions that could selectively inhibit firefly luciferase while having a negligible effect on renilla luciferase in vitro in the dual-reporter gene assay...
  72. Jeon O, Kim C, Laberge R, Demaria M, Rathod S, Vasserot A, et al. Local clearance of senescent cells attenuates the development of post-traumatic osteoarthritis and creates a pro-regenerative environment. Nat Med. 2017;23:775-781 pubmed publisher
    ..harbors a p16INK4a (Cdkn2a) promoter driving the expression of a fusion protein containing synthetic Renilla luciferase and monomeric red fluorescent protein domains, as well as a truncated form of herpes simplex virus 1 ..
  73. Moyle R, Carvalhais L, Pretorius L, Nowak E, Subramaniam G, Dalton Morgan J, et al. An Optimized Transient Dual Luciferase Assay for Quantifying MicroRNA Directed Repression of Targeted Sequences. Front Plant Sci. 2017;8:1631 pubmed publisher
    ..reporter system, where firefly luciferase is used to report the microRNA-target sequence interaction and Renilla luciferase is used as an internal standard to normalize expression between replicates...
  74. Hamdan F, Audet M, Garneau P, Pelletier J, Bouvier M. High-throughput screening of G protein-coupled receptor antagonists using a bioluminescence resonance energy transfer 1-based beta-arrestin2 recruitment assay. J Biomol Screen. 2005;10:463-75 pubmed
    ..One of the developed cell lines coexpressing CCR5-VENUS and beta-arrestin2- Renilla luciferase was then used for high-throughput screening (HTS) for antagonists of the chemokine receptor CCR5, the ..
  75. Paulmurugan R, Gambhir S. An intramolecular folding sensor for imaging estrogen receptor-ligand interactions. Proc Natl Acad Sci U S A. 2006;103:15883-8 pubmed
    ..and validated intramolecular folding sensors encoding various hER-LBD fusion proteins that could lead to split Renilla/firefly luciferase reporter complementation in the presence of the appropriate ligands...
  76. Giodini A, Cresswell P. Hsp90-mediated cytosolic refolding of exogenous proteins internalized by dendritic cells. EMBO J. 2008;27:201-11 pubmed
    ..These observations not only clarify the cellular processes regulating cytosolic access following endocytosis, but also demonstrate that functional proteins can potentially regain their activity in the cytosol of dendritic cells. ..
  77. Stepanyuk G, Liu Z, Vysotski E, Lee J, Rose J, Wang B. Structure based mechanism of the Ca(2+)-induced release of coelenterazine from the Renilla binding protein. Proteins. 2009;74:583-93 pubmed publisher
    The crystal structure of the Ca(2+)-loaded coelenterazine-binding protein from Renilla muelleri in its apo-state has been determined at resolution 1.8 A...
  78. Balasubramanian A, Teramoto T, Kulkarni A, Bhattacharjee A, Padmanabhan R. Antiviral activities of selected antimalarials against dengue virus type 2 and Zika virus. Antiviral Res. 2017;137:141-150 pubmed publisher
    ..AQ) and derivatives, were shown to have potent anti-dengue viral (DENV) activity by using the stable DENV2 Renilla luciferase reporter replicon expressing BHK-21 cells, infectivity (plaque), and the qRT-PCR assays...
  79. Shen Z, Ye G, Deng F, Wang G, Cui M, Fang L, et al. Structural Basis for the Inhibition of Host Gene Expression by Porcine Epidemic Diarrhea Virus nsp1. J Virol. 2018;92: pubmed publisher
    ..Using ribopuromycylation and Renilla luciferase reporter assays, we showed that PEDV nsp1 can dramatically inhibit general host gene expression...
  80. Granier S, Terrillon S, Pascal R, Déméné H, Bouvier M, Guillon G, et al. A cyclic peptide mimicking the third intracellular loop of the V2 vasopressin receptor inhibits signaling through its interaction with receptor dimer and G protein. J Biol Chem. 2004;279:50904-14 pubmed
    ..i3 cyc induced a significant inhibition of the bioluminescence resonance energy-transfer signal between the Renilla reniformis luciferase and the enhanced yellow fluorescent protein fused V2-R...
  81. Kelloniemi J, Mäkinen K, Valkonen J. Three heterologous proteins simultaneously expressed from a chimeric potyvirus: infectivity, stability and the correlation of genome and virion lengths. Virus Res. 2008;135:282-91 pubmed publisher
    ..The genes for green fluorescent protein of Aequoria victoriae ("G"; 714 nucleotides, nt), luciferase of Renilla reniformis ("L", 933 nt) and beta-glucuronidase of Escherichia coli ("U", 1806 nt) were ..
  82. Takahashi Y, Nishikawa M, Kobayashi N, Takakura Y. Gene silencing in primary and metastatic tumors by small interfering RNA delivery in mice: quantitative analysis using melanoma cells expressing firefly and sea pansy luciferases. J Control Release. 2005;105:332-43 pubmed
    ..These results indicate that the both RNAi effectors have a potential to silence target gene in tumor cells in vivo when successfully delivered to tumor cells. ..
  83. Yuan M, Ma X, Jiang T, Gao Y, Cui Y, Zhang C, et al. Prolonged bioluminescence imaging in living cells and mice using novel pro-substrates for Renilla luciferase. Org Biomol Chem. 2017;15:10238-10244 pubmed publisher
    ..In the current work, we designed and synthesized ten novel pro-substrates for Renilla luciferase by introducing ester protecting groups of different sizes into the carbonyl group of the free ..
  84. Mathiesen J, Ulven T, Martini L, Gerlach L, Heinemann A, Kostenis E. Identification of indole derivatives exclusively interfering with a G protein-independent signaling pathway of the prostaglandin D2 receptor CRTH2. Mol Pharmacol. 2005;68:393-402 pubmed
  85. Takai R, Kaneda T, Isogai A, Takayama S, Che F. A new method of defense response analysis using a transient expression system in rice protoplasts. Biosci Biotechnol Biochem. 2007;71:590-3 pubmed
    ..The assay system sensitively detected defense induction by flagellin, which had previously been assigned to a specific elicitor. Our assay system provides a rapid and efficient way to dissect rice defense mechanisms...
  86. Tchurikov N, Kretova O, Moiseeva E, Sosin D. Evidence for RNA synthesis in the intergenic region between enhancer and promoter and its inhibition by insulators in Drosophila melanogaster. Nucleic Acids Res. 2009;37:111-22 pubmed publisher
    ..These results highlight the involvement of RNA-tracking mechanisms in the communications between enhancers and promoters, which are inhibited by insulators...
  87. Stepanyuk G, Unch J, Malikova N, Markova S, Lee J, Vysotski E. Coelenterazine-v ligated to Ca2+-triggered coelenterazine-binding protein is a stable and efficient substrate of the red-shifted mutant of Renilla muelleri luciferase. Anal Bioanal Chem. 2010;398:1809-17 pubmed publisher
    ..shown that the coelenterazine analog, coelenterazine-v, is an efficient substrate for a reaction catalyzed by Renilla luciferase...
  88. Krasitskaya V, Korneeva S, Kudryavtsev A, Markova S, Stepanyuk G, Frank L. Ca(2+)-triggered coelenterazine-binding protein from Renilla as an enzyme-dependent label for binding assay. Anal Bioanal Chem. 2011;401:2573-9 pubmed publisher
    The recombinant Ca(2+)-triggered coelenterazine-binding protein (CBP) from Renilla muelleri was investigated as a biospecifically labeled molecule for in vitro assay applications...
  89. Willibald J, Harder J, Sparrer K, Conzelmann K, Carell T. Click-modified anandamide siRNA enables delivery and gene silencing in neuronal and immune cells. J Am Chem Soc. 2012;134:12330-3 pubmed publisher
    ..In addition, the anandamide conjugates were found to be nontoxic. The reported chemistry and the described properties of the anandamide siRNA extend the possibilities of using siRNA-based gene silencing in neuronal and immune cells...
  90. Kim S, Nishihara R, Citterio D, Suzuki K. Genetically Encoded Molecular Tension Probe for Tracing Protein-Protein Interactions in Mammalian Cells. Bioconjug Chem. 2016;27:354-62 pubmed publisher
    ..This study expands capabilities of luciferases as a tool for analyses of molecular dynamics and cell signaling in living subjects. ..
  91. Harikumar K, Dong M, Cheng Z, Pinon D, Lybrand T, Miller L. Transmembrane segment peptides can disrupt cholecystokinin receptor oligomerization without affecting receptor function. Biochemistry. 2006;45:14706-16 pubmed
    ..Oligomerization of CCK receptors tagged at the carboxyl terminus with Renilla luciferase or yellow fluorescent protein was quantified using bioluminescence resonance energy transfer (BRET)...
  92. Bouzaiene M, Angers A, Anctil M. Immunohistochemical localization of a retinoic acid-like receptor in nerve cells of two colonial anthozoans (Cnidaria). Tissue Cell. 2007;39:123-30 pubmed investigate the presence and cellular distribution of a RXR-like receptor in the sea pansy Renilla koellikeri and in the staghorn coral Acropora millepora (Cnidaria, Anthozoa)...