acanthamoeba castellanii

Summary

Summary: A species of free-living soil amoebae in the family Acanthamoebidae. It can cause ENCEPHALITIS and KERATITIS in humans.

Top Publications

  1. Trzyna W, Legras X, Cordingley J. A type-1 metacaspase from Acanthamoeba castellanii. Microbiol Res. 2008;163:414-23 pubmed
    The complete sequence of a type-1 metacaspase from Acanthamoeba castellanii is reported comprising 478 amino acids...
  2. Wojtkowska M, Szczech N, Stobienia O, Jarmuszkiewicz W, Budzińska M, Kmita H. An inception report on the TOM complex of the Amoeba Acanthamoeba castellanii, a simple model protozoan in mitochondria studies. J Bioenerg Biomembr. 2005;37:261-8 pubmed
    ..The latter involves also the TOM complexes of algae and protozoans. The amoeba Acanthamoeba castellanii is a free-living non-photosynthetic soil protozoan, whose mitochondria share many bioenergetic properties ..
  3. Matz C, Moreno A, Alhede M, Manefield M, Hauser A, Givskov M, et al. Pseudomonas aeruginosa uses type III secretion system to kill biofilm-associated amoebae. ISME J. 2008;2:843-52 pubmed publisher
    ..Studying cocultures of the environmental pathogen Pseudomonas aeruginosa and the amoeba Acanthamoeba castellanii, we found that P...
  4. Sayanova O, Haslam R, Qi B, Lazarus C, Napier J. The alternative pathway C20 Delta8-desaturase from the non-photosynthetic organism Acanthamoeba castellanii is an atypical cytochrome b5-fusion desaturase. FEBS Lett. 2006;580:1946-52 pubmed
    A cDNA encoding a C20 Delta8-desaturase was isolated from the free-living soil amoeba, Acanthamoeba castellanii and functionally characterised by heterologous expression. The open reading frame of the A...
  5. Slocinska M, Szewczyk A, Hryniewiecka L, Kmita H. Benzodiazepine binding to mitochondrial membranes of the amoeba Acanthamoeba castellanii and the yeast Saccharomyces cerevisiae. Acta Biochim Pol. 2004;51:953-62 pubmed
    ..Nevertheless, the MBR ligand binding sites in mitochondria of lower eukaryotes can be applied as useful models in studies on mammalian MBR. ..
  6. Lambrecht E, Bare J, Van Damme I, Bert W, Sabbe K, Houf K. Behavior of Yersinia enterocolitica in the presence of the bacterivorous Acanthamoeba castellanii. Appl Environ Microbiol. 2013;79:6407-13 pubmed publisher
    ..interaction between Yersinia enterocolitica, an important food-borne pathogen, and the free-living amoeba Acanthamoeba castellanii was studied. Several cocultivation assays were set up to assess the resistance of Y. enterocolitica to A...
  7. Polat Z, Tepe B, Vural A. In vitro effectiveness of Thymus sipyleus subsp. sipyleus var. sipyleus on Acanthamoeba castellanii and its cytotoxic potential on corneal cells. Parasitol Res. 2007;101:1551-5 pubmed
    ..sipyleus var. sipyleus from Turkish flora against Acanthamoeba castellanii and also its cytotoxicity on corneal cells in vitro...
  8. Tenant R, Bermudez L. Mycobacterium avium genes upregulated upon infection of Acanthamoeba castellanii demonstrate a common response to the intracellular environment. Curr Microbiol. 2006;52:128-33 pubmed
    ..These results showed that the GFP promoter library in M. smegmatis is a valid system for studying gene upregulation in M. avium systems, and that many M. avium genes are commonly upregulated following macrophage and amoeba infection. ..
  9. Massilamany C, Asojo O, Gangaplara A, Steffen D, Reddy J. Identification of a second mimicry epitope from Acanthamoeba castellanii that induces CNS autoimmunity by generating cross-reactive T cells for MBP 89-101 in SJL mice. Int Immunol. 2011;23:729-39 pubmed publisher
    We had previously reported that Acanthamoeba castellanii (ACA) contains a mimicry epitope for proteolipid protein 139-151 capable of inducing central nervous system (CNS) autoimmunity in SJL/J mice...

More Information

Publications114 found, 100 shown here

  1. Lee X, Reimmann C, Greub G, SUFRIN J, Croxatto A. The Pseudomonas aeruginosa toxin L-2-amino-4-methoxy-trans-3-butenoic acid inhibits growth and induces encystment in Acanthamoeba castellanii. Microbes Infect. 2012;14:268-72 pubmed publisher
    ..evaluate its importance as a potential virulence factor, we tested the host response towards AMB using an Acanthamoeba castellanii cell model. We found that AMB (at concentrations ? 0...
  2. Kim E, Charpentier X, Torres Urquidy O, McEvoy M, Rensing C. The metal efflux island of Legionella pneumophila is not required for survival in macrophages and amoebas. FEMS Microbiol Lett. 2009;301:164-70 pubmed publisher
    ..deletion strains of the metal efflux genes were tested in human THP-1-derived macrophages and amoebal Acanthamoeba castellanii cells and were able to survive and replicate similar to the wild type, suggesting that they do not play a ..
  3. KaraÅ› M, Russa R. New long chain bases in lipophosphonoglycan of Acanthamoeba castellanii. Lipids. 2013;48:639-50 pubmed publisher
    The polymer called lipophosphonoglycan (LPG) was isolated from Acanthamoeba castellanii membranes after exhaustive delipidation and butanol extraction...
  4. Polat Z, Vural A, Tepe B, Cetin A. In vitro amoebicidal activity of four Allium species on Acanthamoeba castellanii and their cytotoxic potentials on corneal cells. Parasitol Res. 2007;101:397-402 pubmed
    ..the in vitro effectiveness of the methanolic extract of four Allium species from Turkey against Acanthamoeba castellanii and its cytotoxicity on corneal cells in vitro...
  5. Douesnard Malo F, Daigle F. Increased persistence of Salmonella enterica serovar Typhi in the presence of Acanthamoeba castellanii. Appl Environ Microbiol. 2011;77:7640-6 pubmed publisher
    ..As the free-living amoeba Acanthamoeba castellanii is an environmental host for many pathogenic bacteria, this study investigates interactions between S...
  6. Tyson J, Pearce M, Vargas P, Bagchi S, Mulhern B, Cianciotto N. Multiple Legionella pneumophila Type II secretion substrates, including a novel protein, contribute to differential infection of the amoebae Acanthamoeba castellanii, Hartmannella vermiformis, and Naegleria lovaniensis. Infect Immun. 2013;81:1399-410 pubmed publisher
    ..pneumophila is required for intracellular infection of host cells, including macrophages and the amoebae Acanthamoeba castellanii and Hartmannella vermiformis. Previous proteomic analysis revealed that T2S by L...
  7. González Robles A, Castañón G, Hernández Ramírez V, Salazar Villatoro L, González Lázaro M, Omaña Molina M, et al. Acanthamoeba castellanii: identification and distribution of actin cytoskeleton. Exp Parasitol. 2008;119:411-7 pubmed publisher
    The presence of the cytoskeleton of Acanthamoeba castellanii was observed by means of cryo-electronmicroscopy and immunofluorescence techniques...
  8. Albers U, Reus K, Shuman H, Hilbi H. The amoebae plate test implicates a paralogue of lpxB in the interaction of Legionella pneumophila with Acanthamoeba castellanii. Microbiology. 2005;151:167-82 pubmed
    ..pneumophila wild-type and icm/dot mutant strains spotted on agar plates in the presence of Acanthamoeba castellanii. In the APT, wild-type L...
  9. Polat Z, Vural A, Ozan F, Tepe B, Ozcelik S, Cetin A. In vitro evaluation of the amoebicidal activity of garlic (Allium sativum) extract on Acanthamoeba castellanii and its cytotoxic potential on corneal cells. J Ocul Pharmacol Ther. 2008;24:8-14 pubmed publisher
    ..Garlic alone, and in combination with other amoebicidal agents, may be used in clinical practices after further investigations. ..
  10. Swida A, Woyda Ploszczyca A, Jarmuszkiewicz W. Redox state of quinone affects sensitivity of Acanthamoeba castellanii mitochondrial uncoupling protein to purine nucleotides. Biochem J. 2008;413:359-67 pubmed publisher
    We studied FFA (free fatty acid)-induced uncoupling activity in Acanthamoeba castellanii mitochondria in the non-phosphorylating state...
  11. Baré J, Sabbe K, Huws S, Vercauteren D, Braeckmans K, van Gremberghe I, et al. Influence of temperature, oxygen and bacterial strain identity on the association of Campylobacter jejuni with Acanthamoeba castellanii. FEMS Microbiol Ecol. 2010;74:371-81 pubmed publisher
    ..strains with different invasion potential towards Caco-2 cells to survive and replicate in the protozoan Acanthamoeba castellanii was tested under simulated in situ conditions (i.e. chicken broiler houses)...
  12. Kicinska A, Swida A, Bednarczyk P, Koszela Piotrowska I, Choma K, Dolowy K, et al. ATP-sensitive potassium channel in mitochondria of the eukaryotic microorganism Acanthamoeba castellanii. J Biol Chem. 2007;282:17433-41 pubmed
    ..a potassium ion transport mechanism in the mitochondrial inner membrane of a lower eukaryotic organism, Acanthamoeba castellanii. We found that substances known to modulate potassium channel activity influenced the bioenergetics of A...
  13. Newton H, Sansom F, Dao J, Cazalet C, Bruggemann H, Albert Weissenberger C, et al. Significant role for ladC in initiation of Legionella pneumophila infection. Infect Immun. 2008;76:3075-85 pubmed publisher
    ..pneumophila and assessed its ability to replicate in mammalian cell lines and Acanthamoeba castellanii. The ladC mutant was recovered in significantly lower numbers than wild-type L...
  14. Polat Z, Savage P, Genberg C. In vitro amoebicidal activity of a ceragenin, cationic steroid antibiotic-13, against Acanthamoeba castellanii and its cytotoxic potential. J Ocul Pharmacol Ther. 2011;27:1-5 pubmed publisher
    ..in vitro effectiveness of CSA-13 with a concentration of 100, 75, 50, and 25? mg/mL on proliferation of Acanthamoeba castellanii trophozoites and cysts and cytotoxic potential...
  15. Snelling W, McKenna J, Lecky D, Dooley J. Survival of Campylobacter jejuni in waterborne protozoa. Appl Environ Microbiol. 2005;71:5560-71 pubmed
    ..An in vitro assay showed that C. jejuni remained viable when internalized by Tetrahymena pyriformis and Acanthamoeba castellanii for significantly longer (up to 36 h) than when they were in purely a planktonic state...
  16. Sayanova O, Haslam R, Guschina I, Lloyd D, Christie W, Harwood J, et al. A bifunctional Delta12,Delta15-desaturase from Acanthamoeba castellanii directs the synthesis of highly unusual n-1 series unsaturated fatty acids. J Biol Chem. 2006;281:36533-41 pubmed
    The free-living soil protozoon Acanthamoeba castellanii synthesizes a range of polyunsaturated fatty acids, the balance of which can be altered by environmental changes...
  17. Kennedy G, Morisaki J, Champion P. Conserved mechanisms of Mycobacterium marinum pathogenesis within the environmental amoeba Acanthamoeba castellanii. Appl Environ Microbiol. 2012;78:2049-52 pubmed publisher
    ..marinum pathogenesis and that M. marinum utilizes actin-based motility in amoebae. Therefore, at least two virulence pathways used by M. marinum in macrophages are conserved during M. marinum infection of amoebae. ..
  18. Shoaib H, Muazzam A, Mir A, Jung S, Matin A. Evaluation of inhibitory potential of some selective methanolic plants extracts on biological characteristics of Acanthamoeba castellanii using human corneal epithelial cells in vitro. Parasitol Res. 2013;112:1179-88 pubmed publisher
    ..determine the efficacy of methanolic plants crude extracts on the viability and biological properties of Acanthamoeba castellanii (T4 genotype) and its cytotoxic effects on human corneal epithelial cells (HCEC)...
  19. Abd H, Saeed A, Weintraub A, Nair G, Sandström G. Vibrio cholerae O1 strains are facultative intracellular bacteria, able to survive and multiply symbiotically inside the aquatic free-living amoeba Acanthamoeba castellanii. FEMS Microbiol Ecol. 2007;60:33-9 pubmed
    ..It has recently been shown that the aquatic and free-living amoeba Acanthamoeba castellanii is not a predator to V. cholerae O139; rather, V...
  20. Abd H, Saeed A, Weintraub A, Sandström G. Vibrio cholerae O139 requires neither capsule nor LPS O side chain to grow inside Acanthamoeba castellanii. J Med Microbiol. 2009;58:125-31 pubmed publisher
    ..the causative agent of cholera, has the ability to grow and survive in the aquatic free-living amoeba Acanthamoeba castellanii. The aim of the present study was to examine the ability of the clinical isolate V...
  21. Moon E, Chung D, Hong Y, Kong H. Atg3-mediated lipidation of Atg8 is involved in encystation of Acanthamoeba. Korean J Parasitol. 2011;49:103-8 pubmed publisher
    ..identified and characterized autophagy-related protein (Atg) 3, a member of Atg8 conjugation system, in Acanthamoeba castellanii (AcAtg3)...
  22. Woyda Ploszczyca A, Jarmuszkiewicz W. Hydroxynonenal-stimulated activity of the uncoupling protein in Acanthamoeba castellanii mitochondria under phosphorylating conditions. Biol Chem. 2013;394:649-58 pubmed publisher
    ..The influence of 4-hydroxy-2-nonenal (HNE), a lipid peroxidation end product, on the activity of the amoeba Acanthamoeba castellanii uncoupling protein (AcUCP) in isolated phosphorylating mitochondria was studied...
  23. Clarke D, Alizadeh H, Niederkorn J. Failure of Acanthamoeba castellanii to produce intraocular infections. Invest Ophthalmol Vis Sci. 2005;46:2472-8 pubmed
    This study examined possible mechanisms to explain why Acanthamoeba castellanii remains restricted to the cornea and rarely produces intraocular infections...
  24. Topalkara A, Vural A, Polat Z, Toker M, Arici M, Ozan F, et al. In vitro amoebicidal activity of propolis on Acanthamoeba castellanii. J Ocul Pharmacol Ther. 2007;23:40-5 pubmed
    ..to investigate the in vitro effects of an ethanolic extract of propolis on the growth and adherence of Acanthamoeba castellanii trophozoites and cysts. The effect of propolis with concentrations of 8.0, 6.0, 5.0, 4.0, 3.0, and 2...
  25. Pickup Z, Pickup R, Parry J. A comparison of the growth and starvation responses of Acanthamoeba castellanii and Hartmannella vermiformis in the presence of suspended and attached Escherichia coli K12. FEMS Microbiol Ecol. 2007;59:556-63 pubmed
    The growth and starvation responses of Acanthamoeba castellanii and Hartmannella vermiformis were investigated in the presence and absence of Escherichia coli on an agar surface or within shaken suspensions...
  26. Declerck P, Behets J, Delaedt Y, Margineanu A, Lammertyn E, Ollevier F. Impact of non-Legionella bacteria on the uptake and intracellular replication of Legionella pneumophila in Acanthamoeba castellanii and Naegleria lovaniensis. Microb Ecol. 2005;50:536-49 pubmed
    ..pneumophila serogroup 1 by the amoebae Acanthamoeba castellanii and Naegleria lovaniensis...
  27. Sissons J, Kim K, Stins M, Jayasekera S, Alsam S, Khan N. Acanthamoeba castellanii induces host cell death via a phosphatidylinositol 3-kinase-dependent mechanism. Infect Immun. 2005;73:2704-8 pubmed
    Granulomatous amoebic encephalitis due to Acanthamoeba castellanii is a serious human infection with fatal consequences, but it is not clear how the circulating amoebae interact with the blood-brain barrier and transmigrate into the ..
  28. Alsam S, Jeong S, Sissons J, Dudley R, Kim K, Khan N. Escherichia coli interactions with Acanthamoeba: a symbiosis with environmental and clinical implications. J Med Microbiol. 2006;55:689-94 pubmed
    ..In this study, Acanthamoeba castellanii (a keratitis isolate belonging to the T4 genotype) was used and its interactions with Escherichia coli (..
  29. Saeed A, Abd H, Edvinsson B, Sandström G. Acanthamoeba castellanii an environmental host for Shigella dysenteriae and Shigella sonnei. Arch Microbiol. 2009;191:83-8 pubmed publisher
    The interaction between Shigella dysenteriae or Shigella sonnei and Acanthamoeba castellanii was studied by viable counts, gentamicin assay and electron microscopy...
  30. Sacramento R, Martins R, Miranda A, Dobroff A, Daffre S, Foronda A, et al. Differential effects of alpha-helical and beta-hairpin antimicrobial peptides against Acanthamoeba castellanii. Parasitology. 2009;136:813-21 pubmed publisher
    ..ability of different types of antimicrobial peptides to promote permeabilization and growth inhibition of Acanthamoeba castellanii trophozoites, which cause eye keratitis...
  31. El Sayed N, Ismail K, Ahmed S, Hetta M. In vitro amoebicidal activity of ethanol extracts of Arachis hypogaea L., Curcuma longa L. and Pancratium maritimum L. on Acanthamoeba castellanii cysts. Parasitol Res. 2012;110:1985-92 pubmed publisher
    b>Acanthamoeba castellanii causes amoebic keratitis which is a painful sight-threatening disease of the eyes...
  32. Bui X, Qvortrup K, Wolff A, Bang D, Creuzenet C. Effect of environmental stress factors on the uptake and survival of Campylobacter jejuni in Acanthamoeba castellanii. BMC Microbiol. 2012;12:232 pubmed publisher
    ..To test this hypothesis, we examined the impact of environmental stress on expression of virulence-associated genes (ciaB, dnaJ, and htrA) of C. jejuni and on its uptake by and intracellular survival within Acanthamoeba castellanii.
  33. Bardill J, Miller J, Vogel J. IcmS-dependent translocation of SdeA into macrophages by the Legionella pneumophila type IV secretion system. Mol Microbiol. 2005;56:90-103 pubmed
    ..the SidE family of proteins interacts with IcmS and is required for full virulence in the protozoan host Acanthamoeba castellanii. Using immunofluorescence microscopy and adenylate cyclase fusions, we show that SdeA is secreted into ..
  34. Ferhat M, Atlan D, Vianney A, Lazzaroni J, Doublet P, Gilbert C. The TolC protein of Legionella pneumophila plays a major role in multi-drug resistance and the early steps of host invasion. PLoS ONE. 2009;4:e7732 pubmed publisher
    ..These findings will be helpful in unraveling the pathogenic mechanisms of L. pneumophila as well as in developing new therapeutic agents affecting the efflux of toxic compounds. ..
  35. Sissons J, Alsam S, Stins M, Rivas A, Morales J, Faull J, et al. Use of in vitro assays to determine effects of human serum on biological characteristics of Acanthamoeba castellanii. J Clin Microbiol. 2006;44:2595-600 pubmed
    ..Conversely, Acanthamoeba phagocytosis was stimulated by serum. ..
  36. Bruggemann H, Hagman A, Jules M, Sismeiro O, Dillies M, Gouyette C, et al. Virulence strategies for infecting phagocytes deduced from the in vivo transcriptional program of Legionella pneumophila. Cell Microbiol. 2006;8:1228-40 pubmed
    ..program of the human pathogen Legionella pneumophila during infection of its natural host, Acanthamoeba castellanii. The biphasic life cycle of L...
  37. Moon E, Chung D, Hong Y, Kong H. Expression levels of encystation mediating factors in fresh strain of Acanthamoeba castellanii cyst ESTs. Exp Parasitol. 2011;127:811-6 pubmed publisher
    ..The information of the expressed genes during encystation in only the fresh strain will provide new clues to understanding the encystation mechanism of encysting protozoa including Acanthamoeba. ..
  38. Rossier O, Dao J, Cianciotto N. The type II secretion system of Legionella pneumophila elaborates two aminopeptidases, as well as a metalloprotease that contributes to differential infection among protozoan hosts. Appl Environ Microbiol. 2008;74:753-61 pubmed
    ..of lapA and lapB mutants to infect human U937 cell macrophages as well as Hartmannella vermiformis and Acanthamoeba castellanii amoebae...
  39. Dudley R, Alsam S, Khan N. The role of proteases in the differentiation of Acanthamoeba castellanii. FEMS Microbiol Lett. 2008;286:9-15 pubmed publisher
    ..However, there is now growing evidence of their involvement in cellular differentiation. Acanthamoeba castellanii of the T4 genotype elaborates a number of proteases, which are inhibited by the serine protease inhibitor ..
  40. Jules M, Buchrieser C. Legionella pneumophila adaptation to intracellular life and the host response: clues from genomics and transcriptomics. FEBS Lett. 2007;581:2829-38 pubmed
    ..feature of Legionella is its dual host system allowing the intracellular growth in protozoa like Acanthamoeba castellanii, and during infection in human alveolar macrophages...
  41. Bui X, Winding A, Qvortrup K, Wolff A, Bang D, Creuzenet C. Survival of Campylobacter jejuni in co-culture with Acanthamoeba castellanii: role of amoeba-mediated depletion of dissolved oxygen. Environ Microbiol. 2012;14:2034-47 pubmed publisher
    ..In this study, we examined its interactions with Acanthamoeba castellanii, a protozoan suspected to serve as a reservoir for bacterial pathogens...
  42. Newton H, Sansom F, Dao J, McAlister A, Sloan J, Cianciotto N, et al. Sel1 repeat protein LpnE is a Legionella pneumophila virulence determinant that influences vacuolar trafficking. Infect Immun. 2007;75:5575-85 pubmed
    ..In further virulence studies here, we show that LpnE is also required for efficient infection of Acanthamoeba castellanii by L. pneumophila and for replication of L. pneumophila in the lungs of A/J mice...
  43. Laskowski Arce M, Orth K. Acanthamoeba castellanii promotes the survival of Vibrio parahaemolyticus. Appl Environ Microbiol. 2008;74:7183-8 pubmed publisher
    ..We investigated the interaction between the protozoan Acanthamoeba castellanii and the bacterium Vibrio parahaemolyticus...
  44. Dudley R, Jarroll E, Khan N. Carbohydrate analysis of Acanthamoeba castellanii. Exp Parasitol. 2009;122:338-43 pubmed publisher
    We analyzed biochemically Acanthamoeba castellanii trophozoites, intact cysts and cyst walls belonging to the T4 genotype using gas chromatography combined with mass spectrometry...
  45. Tamang M, Kim S, Kim S, Kong H, Kim J. Interaction of Acinetobacter baumannii 19606 and 1656-2 with Acanthamoeba castellanii. J Microbiol. 2011;49:841-6 pubmed publisher
    ..baumannii type strain (ATCC 19606(T)). Acanthamoeba castellanii, a single-celled organism, was used as the host model system to study the virulence of A. baumannii...
  46. Zhou X, Elmose J, Call D. Interactions between the environmental pathogen Listeria monocytogenes and a free-living protozoan (Acanthamoeba castellanii). Environ Microbiol. 2007;9:913-22 pubmed
    ..Co-culturing of L. monocytogenes and Acanthamoeba castellanii demonstrated that L...
  47. Moon E, Chung D, Hong Y, Kong H. Differentially expressed genes of Acanthamoeba castellanii during encystation. Korean J Parasitol. 2007;45:283-5 pubmed
    To examine the expressed gene profile during encystation of Acanthamoeba castellanii Castellani, we used differentially expressed gene (DGE) screening by RT-PCR with 20 sets of random primers...
  48. Brindley N, Matin A, Khan N. Acanthamoeba castellanii: high antibody prevalence in racially and ethnically diverse populations. Exp Parasitol. 2009;121:254-6 pubmed publisher
    ..In the present study, we examined secretory IgA antibody to Acanthamoeba castellanii of the T4 genotype in mucosal secretions from 114 individuals of 37 countries, inhabitants and/or ..
  49. Bouyer S, Imbert C, Rodier M, Hechard Y. Long-term survival of Legionella pneumophila associated with Acanthamoeba castellanii vesicles. Environ Microbiol. 2007;9:1341-4 pubmed
    ..pneumophila Lens over 6 months in a poor medium, with or without Acanthamoeba castellanii. The results demonstrated that L...
  50. Ohno A, Kato N, Sakamoto R, Kimura S, Yamaguchi K. Temperature-dependent parasitic relationship between Legionella pneumophila and a free-living amoeba (Acanthamoeba castellanii). Appl Environ Microbiol. 2008;74:4585-8 pubmed publisher
    We analyzed the effects of temperature on the interaction of Legionella pneumophila with Acanthamoeba castellanii. At <20 degrees C, overexpression of type 1 metacaspase, a stimulator of A...
  51. Woyda Ploszczyca A, Jarmuszkiewicz W. Hydroxynonenal, a lipid peroxidation end product, stimulates uncoupling protein activity in Acanthamoeba castellanii mitochondria; the sensitivity of the inducible activity to purine nucleotides depends on the membranous ubiquinone redox state. J Bioenerg Biomembr. 2012;44:525-38 pubmed publisher
    ..and exogenous 4-hydroxy-2-nonenal (HNE), a lipid peroxidation end product, on the activity of the Acanthamoeba castellanii uncoupling protein (AcUCP)...
  52. Goy G, Thomas V, Rimann K, Jaton K, Prod hom G, Greub G. The Neff strain of Acanthamoeba castellanii, a tool for testing the virulence of Mycobacterium kansasii. Res Microbiol. 2007;158:393-7 pubmed
    ..may use free-living amoebae as a training ground to select virulence traits, we wondered whether the Acanthamoeba castellanii amoeba could be used to determine the virulence of these intracellular bacteria...
  53. Gawryluk R, Gray M. Evidence for an early evolutionary emergence of gamma-type carbonic anhydrases as components of mitochondrial respiratory complex I. BMC Evol Biol. 2010;10:176 pubmed publisher
    ..However, relatively little is known about CI composition in the unicellular protists, the characterizations of which are essential to our understanding of CI evolution...
  54. Yousuf F, Siddiqui R, Khan N. Acanthamoeba castellanii of the T4 genotype is a potential environmental host for Enterobacter aerogenes and Aeromonas hydrophila. Parasit Vectors. 2013;6:169 pubmed publisher
    ..and Escherichia coli. In this capacity, Acanthamoeba has been suggested as a vector in the transmission of bacterial pathogens to the susceptible hosts...
  55. Galka F, Wai S, Kusch H, Engelmann S, Hecker M, Schmeck B, et al. Proteomic characterization of the whole secretome of Legionella pneumophila and functional analysis of outer membrane vesicles. Infect Immun. 2008;76:1825-36 pubmed publisher
    ..On the basis of these new findings, we discuss the relevance of protein sorting and compartmentalization of virulence factors, as well as environmental aspects of the vesicle-mediated secretion...
  56. Moon E, Chung D, Hong Y, Kong H. Autophagy protein 8 mediating autophagosome in encysting Acanthamoeba. Mol Biochem Parasitol. 2009;168:43-8 pubmed publisher
    ..The present study identified and characterized autophagy protein 8 in Acanthamoeba castellanii an amphizoic amoeba causing granulomatous amoebic encephalitis and amoebic keratitis in humans...
  57. Chomicz L, Padzik M, Graczyk Z, Starosciak B, Graczyk T, Naprawska A, et al. Acanthamoeba castellanii: in vitro effects of selected biological, physical and chemical factors. Exp Parasitol. 2010;126:103-5 pubmed publisher
    Trophozoites and cysts of free-living Acanthamoeba castellanii present a serious risk to human health as causative agents of human diseases such as fatal granulomatous amoebic encephalitis and Acanthamoeba keratitis that is reported from ..
  58. Otri A, Mohammed I, Abedin A, Cao Z, Hopkinson A, Panjwani N, et al. Antimicrobial peptides expression by ocular surface cells in response to Acanthamoeba castellanii: an in vitro study. Br J Ophthalmol. 2010;94:1523-7 pubmed publisher
    ..study the gene expression of the ocular AMPs in human corneal limbal epithelial cells stimulated with Acanthamoeba castellanii (AC)...
  59. Siddiqui R, Malik H, Sagheer M, Jung S, Khan N. The type III secretion system is involved in Escherichia coli K1 interactions with Acanthamoeba. Exp Parasitol. 2011;128:409-13 pubmed publisher
    ..coli strain EC10 exhibited defects in the invasion and intracellular survival in Acanthamoeba castellanii (a keratitis isolate) compared to its parent strain...
  60. Unger C, Luck C. Inhibitory effects of silver ions on Legionella pneumophila grown on agar, intracellular in Acanthamoeba castellanii and in artificial biofilms. J Appl Microbiol. 2012;112:1212-9 pubmed publisher
    ..We undertook a series of experiments to investigate the susceptibility of Legionella pneumophila grown under extracellular and intracellular conditions and other water-related bacteria to silver ions...
  61. Kim J, Na B, Song K, Park M, Park Y, Kim T. Functional expression and characterization of an iron-containing superoxide dismutase of Acanthamoeba castellanii. Parasitol Res. 2012;111:1673-82 pubmed
    ..In this study, we identified a gene encoding iron superoxide dismutase of Acanthamoeba castellanii (AcFe-SOD) and characterized biochemical and functional properties of the recombinant enzyme...
  62. Alsam S, Sissons J, Dudley R, Khan N. Mechanisms associated with Acanthamoeba castellanii (T4) phagocytosis. Parasitol Res. 2005;96:402-9 pubmed
    ..In addition, we showed that phosphatidylinositol 3-kinase (PI3K) plays an important role in Acanthamoeba uptake of E. coli. Role of mannose-binding protein in Acanthamoeba phagocytosis is discussed further...
  63. Snelling W, Stern N, Lowery C, Moore J, Gibbons E, Baker C, et al. Colonization of broilers by Campylobacter jejuni internalized within Acanthamoeba castellanii. Arch Microbiol. 2008;189:175-9 pubmed
    ..jejuni, respectively. After 1, 3, 7 and 14 days post challenge, seven birds from each unit were examined for C. jejuni colonization. For the first time we report that intra-amoeba C. jejuni colonized broilers...
  64. Chrisman C, Alvarez M, Casadevall A. Phagocytosis of Cryptococcus neoformans by, and nonlytic exocytosis from, Acanthamoeba castellanii. Appl Environ Microbiol. 2010;76:6056-62 pubmed publisher
    ..In this study, we investigated the interaction of C. neoformans with the soil amoeba Acanthamoeba castellanii. Comparison of phagocytic efficiency of the wild type, nonencapsulated mutants, and complemented strains ..
  65. Woyda Ploszczyca A, Jarmuszkiewicz W. Ubiquinol (QH(2)) functions as a negative regulator of purine nucleotide inhibition of Acanthamoeba castellanii mitochondrial uncoupling protein. Biochim Biophys Acta. 2011;1807:42-52 pubmed publisher
    ..nucleotides on the free fatty acid-induced uncoupling protein (UCP) activity in non-phosphorylating Acanthamoeba castellanii mitochondria when the membranous ubiquinone (Q) redox state was varied...
  66. Mogoa E, Bodet C, Morel F, Rodier M, Legube B, Hechard Y. Cellular response of the amoeba Acanthamoeba castellanii to chlorine, chlorine dioxide, and monochloramine treatments. Appl Environ Microbiol. 2011;77:4974-80 pubmed publisher
    b>Acanthamoeba castellanii is a free-living amoebae commonly found in water systems. Free-living amoebae might be pathogenic but are also known to bear phagocytosis-resistant bacteria, protecting these bacteria from water treatments...
  67. Malatyali E, Tepe B, Degerli S, Berk S, Akpulat H. In vitro amoebicidal activity of four Peucedanum species on Acanthamoeba castellanii cysts and trophozoites. Parasitol Res. 2012;110:167-74 pubmed publisher
    ..At the same concentration, 51% of the cysts were killed by the extract in the 72nd hour. As expected, cysts were found more resistant to the extracts than the trophozoites...
  68. Anderson I, Watkins R, Samuelson J, Spencer D, Majoros W, Gray M, et al. Gene discovery in the Acanthamoeba castellanii genome. Protist. 2005;156:203-14 pubmed
    b>Acanthamoeba castellanii is a free-living amoeba found in soil, freshwater, and marine environments and an important predator of bacteria...
  69. Vincent C, Vogel J. The Legionella pneumophila IcmS-LvgA protein complex is important for Dot/Icm-dependent intracellular growth. Mol Microbiol. 2006;61:596-613 pubmed
    ..lvgA causes a minimal defect in growth in the human monocytic cell line U937 and the environmental host Acanthamoeba castellanii. However, the lvgA mutant was severely attenuated for intracellular growth of L...
  70. Watkins R, Gray M. Sampling gene diversity across the supergroup Amoebozoa: large EST data sets from Acanthamoeba castellanii, Hartmannella vermiformis, Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba sp. Protist. 2008;159:269-81 pubmed publisher
    ..of EST data for five taxa within the eukaryotic supergroup Amoebozoa, including two free-living amoebae (Acanthamoeba castellanii, Hartmannella vermiformis) and three slime molds (Physarum polycephalum, Hyperamoeba dachnaya and ..
  71. Goy G, Greub G. Antibiotic susceptibility of Waddlia chondrophila in Acanthamoeba castellanii amoebae. Antimicrob Agents Chemother. 2009;53:2663-6 pubmed publisher
    ..W. chondrophila was susceptible to doxycycline and azithromycin but resistant to beta-lactams and fluoroquinolones...
  72. Pearce M, Cianciotto N. Legionella pneumophila secretes an endoglucanase that belongs to the family-5 of glycosyl hydrolases and is dependent upon type II secretion. FEMS Microbiol Lett. 2009;300:256-64 pubmed publisher
    ..Overall, this study represents the first documentation of an endoglucanase (EC 3.2.1.4) being produced by a strain of Legionella...
  73. Mogoa E, Bodet C, Legube B, Hechard Y. Acanthamoeba castellanii: cellular changes induced by chlorination. Exp Parasitol. 2010;126:97-102 pubmed publisher
    ..In conclusion, depending on the concentration used, chlorination leads to many cellular effects on Acanthamoeba that could well arise in cell inactivation...
  74. Eylert E, Herrmann V, Jules M, Gillmaier N, Lautner M, Buchrieser C, et al. Isotopologue profiling of Legionella pneumophila: role of serine and glucose as carbon substrates. J Biol Chem. 2010;285:22232-43 pubmed publisher
    ..significant levels and was slowly outcompeted by the wild type strain in successive rounds of infection in Acanthamoeba castellanii, indicating the importance of this enzyme and of carbohydrate usage in general for the life cycle of Lp.
  75. Moon E, Xuan Y, Chung D, Hong Y, Kong H. Microarray analysis of differentially expressed genes between cysts and trophozoites of Acanthamoeba castellanii. Korean J Parasitol. 2011;49:341-7 pubmed publisher
    ..The present study provides important clues for the identification and characterization of encystation mediating factors of Acanthamoeba...
  76. Alsam S, Sissons J, Jayasekera S, Khan N. Extracellular proteases of Acanthamoeba castellanii (encephalitis isolate belonging to T1 genotype) contribute to increased permeability in an in vitro model of the human blood-brain barrier. J Infect. 2005;51:150-6 pubmed
    ..Objectives of this study were to determine the effects of Acanthamoeba on the permeability of an in vitro blood-brain barrier model and factors contributing to these changes...
  77. Feldman M, Zusman T, Hagag S, Segal G. Coevolution between nonhomologous but functionally similar proteins and their conserved partners in the Legionella pathogenesis system. Proc Natl Acad Sci U S A. 2005;102:12206-11 pubmed
    ..We hypothesize that this gene variation, which is probably mediated by positive selection, plays an important role in the evolutionary arms race between the protozoan host cell and the pathogen...
  78. Massilamany C, Steffen D, Reddy J. An epitope from Acanthamoeba castellanii that cross-react with proteolipid protein 139-151-reactive T cells induces autoimmune encephalomyelitis in SJL mice. J Neuroimmunol. 2010;219:17-24 pubmed publisher
    We report here that an epitope (aa, 83-95) derived from Acanthamoeba castellanii (ACA) induces clinical signs of experimental autoimmune encephalomyelitis (EAE) in SJL/J mice reminiscent of the disease induced with myelin proteolipid ..
  79. Leitsch D, Köhsler M, Marchetti Deschmann M, Deutsch A, Allmaier G, Duchene M, et al. Major role for cysteine proteases during the early phase of Acanthamoeba castellanii encystment. Eukaryot Cell. 2010;9:611-8 pubmed publisher
    b>Acanthamoeba castellanii is a facultative pathogen that has a two-stage life cycle comprising the vegetatively growing trophozoite stage and the dormant cyst stage...
  80. Gawryluk R, Chisholm K, Pinto D, Gray M. Composition of the mitochondrial electron transport chain in acanthamoeba castellanii: structural and evolutionary insights. Biochim Biophys Acta. 2012;1817:2027-37 pubmed publisher
    ..We present an analysis of the ETC proteome from Acanthamoeba castellanii, an ecologically, medically and evolutionarily important member of Amoebozoa (sister to Opisthokonta)...
  81. Saheb E, Trzyna W, Bush J. An Acanthamoeba castellanii metacaspase associates with the contractile vacuole and functions in osmoregulation. Exp Parasitol. 2013;133:314-26 pubmed publisher
    b>Acanthamoeba castellanii is a free-living protozoan. Some strains are opportunistic pathogens. A type-I metacaspase was identified in A. castellanii (Acmcp) and was shown to be expressed through the encystation process...
  82. Baig A, Iqbal J, Khan N. In vitro efficacies of clinically available drugs against growth and viability of an Acanthamoeba castellanii keratitis isolate belonging to the T4 genotype. Antimicrob Agents Chemother. 2013;57:3561-7 pubmed publisher
    ..biochemical pathways; ion channels; and cellular pumps were tested against a keratitis isolate of Acanthamoeba castellanii belonging to the T4 genotype...
  83. Chavez Munguia B, Omaña Molina M, González Lázaro M, González Robles A, Bonilla P, Martinez Palomo A. Ultrastructural study of encystation and excystation in Acanthamoeba castellanii. J Eukaryot Microbiol. 2005;52:153-8 pubmed
    Encystation and excystation of Acanthamoeba castellanii were studied by transmission electron microscopy. The differentiation process was induced in asynchronous cultures grown axenically...
  84. Belyi Y, Tabakova I, Stahl M, Aktories K. Lgt: a family of cytotoxic glucosyltransferases produced by Legionella pneumophila. J Bacteriol. 2008;190:3026-35 pubmed publisher
    ..Expression studies on L. pneumophila grown in broth medium or in Acanthamoeba castellanii revealed that production of Lgt1 was maximal at stationary phase of broth culture or during the late ..
  85. Abd H, Wretlind B, Saeed A, Idsund E, Hultenby K, Sandström G. Pseudomonas aeruginosa utilises its type III secretion system to kill the free-living amoeba Acanthamoeba castellanii. J Eukaryot Microbiol. 2008;55:235-43 pubmed publisher
    ..The aim of this study was to examine interaction of TTSS effector proteins of P. aeruginosa PA103 with Acanthamoeba castellanii by co-cultivation, viable count, eosin staining, electron microscopy, apoptosis assay, and statistical ..
  86. Tiaden A, Spirig T, Carranza P, Bruggemann H, Riedel K, Eberl L, et al. Synergistic contribution of the Legionella pneumophila lqs genes to pathogen-host interactions. J Bacteriol. 2008;190:7532-47 pubmed publisher
    ..A proteome analysis revealed that a set of Icm/Dot substrates is not produced in the absence of the lqs gene cluster, which confirms the findings from DNA microarray assays and mirrors the virulence phenotype of the lqs mutant strain...
  87. El Etr S, Margolis J, Monack D, Robison R, Cohen M, Moore E, et al. Francisella tularensis type A strains cause the rapid encystment of Acanthamoeba castellanii and survive in amoebal cysts for three weeks postinfection. Appl Environ Microbiol. 2009;75:7488-500 pubmed publisher
    ..In this study, the ability of virulent F. tularensis strains to survive and replicate in the amoeba Acanthamoeba castellanii was explored. We observe that A. castellanii trophozoites rapidly encyst in response to F...
  88. Cateau E, Verdon J, Fernandez B, Hechard Y, Rodier M. Acanthamoeba sp. promotes the survival and growth of Acinetobacter baumanii. FEMS Microbiol Lett. 2011;319:19-25 pubmed publisher
    ..water were co-cultivated with the trophozoites or supernatants of two free-living amoebae strains: Acanthamoeba castellanii or Acanthamoeba culbertsoni...
  89. Song S, Han B, Moon E, Lee Y, Yu H, Jha B, et al. Autophagy protein 16-mediated autophagy is required for the encystation of Acanthamoeba castellanii. Mol Biochem Parasitol. 2012;183:158-65 pubmed publisher
    ..Moreover, most mitochondria remained undigested in these knockdown cells. Taken together, these results indicate that AcAtg16 is involved in autophagosome formation and plays an essential role in the encystation of Acanthamoeba...
  90. Siddiqui R, Iqbal J, Maugueret M, Khan N. The role of Src kinase in the biology and pathogenesis of Acanthamoeba castellanii. Parasit Vectors. 2012;5:112 pubmed publisher
    ..Here, we evaluated the role of Src, a non-receptor protein tyrosine kinase in the biology and pathogenesis of Acanthamoeba...
  91. Chekabab S, Daigle F, Charette S, Dozois C, Harel J. Survival of enterohemorrhagic Escherichia coli in the presence of Acanthamoeba castellanii and its dependence on Pho regulon. Microbiologyopen. 2012;1:427-37 pubmed publisher
    ..In this study, the interaction between EHEC and the predacious protozoa Acanthamoeba castellanii was investigated...
  92. Aqeel Y, Siddiqui R, Khan N. Silencing of xylose isomerase and cellulose synthase by siRNA inhibits encystation in Acanthamoeba castellanii. Parasitol Res. 2013;112:1221-7 pubmed publisher
    ..The carbohydrate linkage analysis of cyst walls of Acanthamoeba castellanii showed variously linked sugar residues, including xylofuranose/xylopyranose, glucopyranose, mannopyranose,..