acanthamoeba

Summary

Summary: A genus of free-living soil amoebae that produces no flagellate stage. Its organisms are pathogens for several infections in humans and have been found in the eye, bone, brain, and respiratory tract.

Top Publications

  1. Akya A, Pointon A, Thomas C. Listeria monocytogenes does not survive ingestion by Acanthamoeba polyphaga. Microbiology. 2010;156:809-18 pubmed publisher
    ..monocytogenes strains with Acanthamoeba polyphaga ACO12. Survival of L...
  2. Barros J, Mascaro V, Lowen M, Martins M, Foronda A. [Diagnosis of Acanthamoeba corneal infection by impression cytology: case report]. Arq Bras Oftalmol. 2007;70:343-6 pubmed
    To describe three cases of corneal infection due to Acanthamoeba sp in which was possible to detect Acanthamoeba sp cysts by the corneal impression cytology technique...
  3. Tsibidis G, Burroughs N, Gaze W, Wellington E. Semi-automated Acanthamoeba polyphaga detection and computation of Salmonella typhimurium concentration in spatio-temporal images. Micron. 2011;42:911-20 pubmed publisher
    ..To accelerate organism identification, correct classification errors and attain quantitative details of all objects a custom written Graphical User Interfaces has also been developed. ..
  4. Siddiqui R, Khan N. Biology and pathogenesis of Acanthamoeba. Parasit Vectors. 2012;5:6 pubmed publisher
    b>Acanthamoeba is a free-living protist pathogen, capable of causing a blinding keratitis and fatal granulomatous encephalitis...
  5. Matsumoto Y, Dogru M, Sato E, Katono Y, Uchino Y, Shimmura S, et al. The application of in vivo confocal scanning laser microscopy in the management of Acanthamoeba keratitis. Mol Vis. 2007;13:1319-26 pubmed
    ..confocal microscopy (Heidelberg Retina Tomograph II, Rostock Cornea Module, HRTII-RCM) in the management of Acanthamoeba keratitis (AK)...
  6. Jeong H, Lee S, Kim J, Xuan Y, Lee K, Park S, et al. Acanthamoeba: keratopathogenicity of isolates from domestic tap water in Korea. Exp Parasitol. 2007;117:357-67 pubmed
    In a previous study, we reported on the contamination rate of free living amoeba, including Acanthamoeba, isolated from contact lens storage cases (CLSC) and domestic tap water in Korea...
  7. Ledee D, Iovieno A, Miller D, Mandal N, Diaz M, Fell J, et al. Molecular identification of t4 and t5 genotypes in isolates from acanthamoeba keratitis patients. J Clin Microbiol. 2009;47:1458-62 pubmed publisher
    b>Acanthamoeba keratitis (AK) is a rare but sight-threatening ocular infection. Outbreaks have been associated with contaminated water and contact lens wear. The epidemiology and pathology may be associated with unique genotypes...
  8. Coulon C, Collignon A, McDonnell G, Thomas V. Resistance of Acanthamoeba cysts to disinfection treatments used in health care settings. J Clin Microbiol. 2010;48:2689-97 pubmed publisher
    Free-living amoebae that belong to the genus Acanthamoeba are widespread in the environment, including water. They are responsible for human infections and can host pathogenic microorganisms...
  9. Booton G, Joslin C, Shoff M, Tu E, Kelly D, Fuerst P. Genotypic identification of Acanthamoeba sp. isolates associated with an outbreak of acanthamoeba keratitis. Cornea. 2009;28:673-6 pubmed publisher
    To determine whether increased rates of Acanthamoeba keratitis (AK) are due to changes in municipal water treatment or to emergence of a more pathogenic strain of Acanthamoeba...

More Information

Publications154 found, 100 shown here

  1. Fürnkranz U, Nagl M, Gottardi W, Köhsler M, Aspöck H, Walochnik J. Cytotoxic activity of N-chlorotaurine on Acanthamoeba spp. Antimicrob Agents Chemother. 2008;52:470-6 pubmed
    b>Acanthamoeba spp...
  2. Heinz E, Kolarov I, Kastner C, Toenshoff E, Wagner M, Horn M. An Acanthamoeba sp. containing two phylogenetically different bacterial endosymbionts. Environ Microbiol. 2007;9:1604-9 pubmed
    ..They frequently contain obligate intracellular bacterial symbionts, which show a worldwide distribution. All Acanthamoeba spp...
  3. Lorenzo Morales J, Martinez Carretero E, Batista N, Alvarez Marin J, Bahaya Y, Walochnik J, et al. Early diagnosis of amoebic keratitis due to a mixed infection with Acanthamoeba and Hartmannella. Parasitol Res. 2007;102:167-9 pubmed
    A mixed keratitis due to Acanthamoeba and Hartmannella species is reported. The patient was a soft contact lens wearer. Early diagnosis was achieved by polymerase chain reaction and culture...
  4. Al Khodor S, Price C, Habyarimana F, Kalia A, Abu Kwaik Y. A Dot/Icm-translocated ankyrin protein of Legionella pneumophila is required for intracellular proliferation within human macrophages and protozoa. Mol Microbiol. 2008;70:908-23 pubmed publisher
    ..defect in intracellular replication within human monocyte-derived macrophages (hMDMs), U937 macrophages and Acanthamoeba polyphaga...
  5. Costa A, Castro E, Ferreira G, Furst C, Crozeta M, Thomaz Soccol V. Characterization of acanthamoeba isolates from dust of a public hospital in Curitiba, Paraná, Brazil. J Eukaryot Microbiol. 2010;57:70-5 pubmed publisher
    Occurrence of Acanthamoeba in the hospital environment may represent a health risk for patients, since these organisms can cause severe opportunistic illness, such as keratitis, and also can harbor pathogenic agents...
  6. Mba Medie F, Ben Salah I, Henrissat B, Raoult D, Drancourt M. Mycobacterium tuberculosis complex mycobacteria as amoeba-resistant organisms. PLoS ONE. 2011;6:e20499 pubmed publisher
    ..7±2% of M. bovis and 11.2±0.5% of Mycobacterium avium control organisms were phagocytized by Acanthamoeba polyphaga, a ratio significantly higher for M. canettii (P?=?0...
  7. Chusattayanond A, Boonsilp S, Kasisit J, Boonmee A, Warit S. Thai Acanthamoeba isolate (T4) induced apoptotic death in neuroblastoma cells via the Bax-mediated pathway. Parasitol Int. 2010;59:512-6 pubmed publisher
    A Thai Acanthamoeba isolate named AS recovered from a corneal scraping of a keratitis patient was genotypically determined as T4...
  8. Kao P, Hsu B, Chen N, Huang K, Huang S, King K, et al. Isolation and identification of Acanthamoeba species from thermal spring environments in southern Taiwan. Exp Parasitol. 2012;130:354-8 pubmed publisher
    b>Acanthamoeba species are free-living amoebae found in a range of environments...
  9. Ueki N, Eguchi H, Oogi Y, Shiota H, Yamane S, Umazume H, et al. Three cases of Acanthamoeba keratitis diagnosed and treated in the early stage. J Med Invest. 2009;56:166-9 pubmed
    b>Acanthamoeba keratitis (AK) is a severe infectious corneal ulcer that usually occurs in contact lens wearers...
  10. Gupta D, Panda G, Bakhshi S. Successful treatment of acanthamoeba meningoencephalitis during induction therapy of childhood acute lymphoblastic leukemia. Pediatr Blood Cancer. 2008;50:1292-3 pubmed publisher
  11. Akya A, Pointon A, Thomas C. Mechanism involved in phagocytosis and killing of Listeria monocytogenes by Acanthamoeba polyphaga. Parasitol Res. 2009;105:1375-83 pubmed publisher
    ..However, Acanthamoeba polyphaga trophozoites phagocytose and rapidly degrade Listeria cells...
  12. Lorenzo Morales J, López Darias M, Martinez Carretero E, Valladares B. Isolation of potentially pathogenic strains of Acanthamoeba in wild squirrels from the Canary Islands and Morocco. Exp Parasitol. 2007;117:74-9 pubmed
    ..a survey was conducted in order to determine the presence and pathogenic potential of free-living amoebae of Acanthamoeba genus in a population of Barbary Ground squirrels in Fuerteventura Island (Canary Islands, Spain) and Morocco...
  13. Mutsafi Y, Zauberman N, Sabanay I, Minsky A. Vaccinia-like cytoplasmic replication of the giant Mimivirus. Proc Natl Acad Sci U S A. 2010;107:5978-82 pubmed publisher
    ..diverse imaging techniques, we show that the infection cycle of the largest virus currently identified, the Acanthamoeba polyphaga Mimivirus, similarly occurs exclusively in the host cytoplasm...
  14. Maubon D, Dubosson M, Chiquet C, Yera H, Brenier Pinchart M, Cornet M, et al. A one-step multiplex PCR for acanthamoeba keratitis diagnosis and quality samples control. Invest Ophthalmol Vis Sci. 2012;53:2866-72 pubmed publisher
    As the number of cases of Acanthamoeba spp. keratitis (AK) is constantly growing, new diagnostic tools are needed to confirm and guide ophthalmologists in this clinically problematic diagnosis...
  15. McBride J, Mullen A, Carter K, Roberts C. Differential cytotoxicity of phospholipid analogues to pathogenic Acanthamoeba species and mammalian cells. J Antimicrob Chemother. 2007;60:521-5 pubmed
    ..studies have reported the ability of several phospholipid analogues to successfully inhibit the growth of Acanthamoeba species in vitro...
  16. Shiraishi A, Uno T, Oka N, Hara Y, Yamaguchi M, Ohashi Y. In vivo and in vitro laser confocal microscopy to diagnose acanthamoeba keratitis. Cornea. 2010;29:861-5 pubmed publisher
    To determine the effectiveness of laser confocal microscopy in identifying Acanthamoeba cysts and trophozoites in the cornea of patients with Acanthamoeba keratitis (AK) and to evaluate its effectiveness in following AK after treatment...
  17. Sampo A, Matsuo J, Yamane C, Yagita K, Nakamura S, Shouji N, et al. High-temperature adapted primitive Protochlamydia found in Acanthamoeba isolated from a hot spring can grow in immortalized human epithelial HEp-2 cells. Environ Microbiol. 2014;16:486-97 pubmed publisher
    ..temperature barriers to adapt to human cells, we characterized a primitive chlamydia found in HS-T3 amoebae (Acanthamoeba) isolated from a hot spring. Phylogenetic analysis revealed the primitive species to be Protochlamydia...
  18. Henriquez F, Ingram P, Muench S, Rice D, Roberts C. Molecular basis for resistance of acanthamoeba tubulins to all major classes of antitubulin compounds. Antimicrob Agents Chemother. 2008;52:1133-5 pubmed
    ..We demonstrate that Acanthamoeba spp. are resistant to five antimicrotubule compounds, unlike any other eukaryote studied so far...
  19. Shoff M, Rogerson A, Kessler K, Schatz S, Seal D. Prevalence of Acanthamoeba and other naked amoebae in South Florida domestic water. J Water Health. 2008;6:99-104 pubmed
    ..The presence of amoebae in samples was based on an enrichment cultivation method appropriate for Acanthamoeba. Amoebae were identified using diagnostic features discernable by light microscopy...
  20. Walochnik J, Aichelburg A, Assadian O, Steuer A, Visvesvara G, Vetter N, et al. Granulomatous amoebic encephalitis caused by Acanthamoeba amoebae of genotype T2 in a human immunodeficiency virus-negative patient. J Clin Microbiol. 2008;46:338-40 pubmed
    b>Acanthamoeba amoebae of genotype T2 were identified as the causative agent of Acanthamoeba skin lesions and granulomatous amoebic encephalitis (GAE) in a human immunodeficiency virus-negative patient with underlying tuberculosis...
  21. Kaushal V, Chhina D, Kumar R, Pannu H, Dhooria H, Chhina R. Acanthamoeba encephalitis. Indian J Med Microbiol. 2008;26:182-4 pubmed
    Central nervous system infection with free-living amoebae is rare. We present a fatal case of Acanthamoeba encephalitis in a 63-year-old female from India where acanthamoebae were demonstrated and cultured from CSF...
  22. Boggild A, Martin D, Lee T, Yu B, Low D. Laboratory diagnosis of amoebic keratitis: comparison of four diagnostic methods for different types of clinical specimens. J Clin Microbiol. 2009;47:1314-8 pubmed publisher
    ..for acanthamoebae by direct smear analysis, culture, and PCR using two different primer sets specific for Acanthamoeba ribosomal DNA...
  23. Chan L, Mak J, Low Y, Koh T, Ithoi I, Mohamed S. Isolation and characterization of Acanthamoeba spp. from air-conditioners in Kuala Lumpur, Malaysia. Acta Trop. 2011;117:23-30 pubmed publisher
    During a study on the quality of the indoor environment, Acanthamoeba spp. were detected in 20 out of 87 dust samples collected from air-conditioners installed in a four-story campus building located in Kuala Lumpur, Malaysia...
  24. Huang S, Hsu B, Chen N, Huang C, Huang K, Chen J, et al. Isolation and identification of Legionella and their host amoebae from weak alkaline carbonate spring water using a culture method combined with PCR. Parasitol Res. 2011;109:1233-41 pubmed publisher
    ..Moreover, we also investigated the existence of Acanthamoeba, Hartmannella, and Naegleria, ubiquitous FLA in aquatic environments, to identify the correlations between ..
  25. Khairnar K, Tamber G, Ralevski F, Pillai D. Comparison of molecular diagnostic methods for the detection of Acanthamoeba spp. from clinical specimens submitted for keratitis. Diagn Microbiol Infect Dis. 2011;70:499-506 pubmed publisher
    b>Acanthamoeba spp. are responsible for a significant annual number of keratitis (AK) cases leading to vision-threatening disease worldwide. Current methods rely on direct examination of specimens by microscopy and/or culture...
  26. Duarte J, Furst C, Klisiowicz D, Klassen G, Costa A. Morphological, genotypic, and physiological characterization of Acanthamoeba isolates from keratitis patients and the domestic environment in Vitoria, Espírito Santo, Brazil. Exp Parasitol. 2013;135:9-14 pubmed publisher
    Amoebae of the genus Acanthamoeba are free-living protozoa that can cause granulomatous encephalitis and keratitis in humans...
  27. Meersseman W, Lagrou K, Sciot R, De Jonckheere J, Haberler C, Walochnik J, et al. Rapidly fatal Acanthamoeba encephalitis and treatment of cryoglobulinemia. Emerg Infect Dis. 2007;13:469-71 pubmed
    ..treated with rituximab, plasmapheresis, and steroids; a case of fatal meningoencephalitis caused by Acanthamoeba spp. then developed...
  28. Shin S, Roy C. Host cell processes that influence the intracellular survival of Legionella pneumophila. Cell Microbiol. 2008;10:1209-20 pubmed publisher
    ..In this review, we discuss how L. pneumophila manipulates host cells, as well as host cell processes that either facilitate or impede its intracellular survival. ..
  29. Zhao G, Sun S, Zhao J, Xie L. Genotyping of Acanthamoeba isolates and clinical characteristics of patients with Acanthamoeba keratitis in China. J Med Microbiol. 2010;59:462-6 pubmed publisher
    b>Acanthamoeba keratitis (AK) is a sight-threatening corneal infection, the epidemiology of which is related to the specific genotype of Acanthamoeba...
  30. Zuchero J. In vitro actin assembly assays and purification from Acanthamoeba. Methods Mol Biol. 2007;370:213-26 pubmed
    ..In addition, fluorescently labeled phalloidin can be used to visualize the filaments that are created in vitro to give insight into how these proteins influence actin filament superstructure. ..
  31. Barete S, Combes A, De Jonckheere J, Datry A, Varnous S, Martinez V, et al. Fatal disseminated Acanthamoeba lenticulata infection in a heart transplant patient. Emerg Infect Dis. 2007;13:736-8 pubmed
    We report a fatal case of disseminated acanthamebiasis caused by Acanthamoeba lenticulata (genotype T5) in a 39-year-old heart transplant recipient...
  32. Yera H, Zamfir O, Bourcier T, Viscogliosi E, Noel C, Dupouy Camet J, et al. The genotypic characterisation of Acanthamoeba isolates from human ocular samples. Br J Ophthalmol. 2008;92:1139-41 pubmed publisher
    We characterised 37 amoebae cultured from corneal scrapings, contact lenses or lens case solutions of patients with suspected Acanthamoeba keratitis.
  33. Polat Z, Obwaller A, Vural A, Walochnik J. Efficacy of miltefosine for topical treatment of Acanthamoeba keratitis in Syrian hamsters. Parasitol Res. 2012;110:515-20 pubmed publisher
    b>Acanthamoeba keratitis is a painful corneal infection and difficult to treat because no sufficiently efficient drug has yet been available...
  34. Ibrahim Y, Boase D, Cree I. Factors affecting the epidemiology of Acanthamoeba keratitis. Ophthalmic Epidemiol. 2007;14:53-60 pubmed
    Despite being a relatively rare disease in comparison with other forms of infectious keratitis, Acanthamoeba keratitis is a potentially blinding disease...
  35. Lorenzo Morales J, Kliescikova J, Martinez Carretero E, De Pablos L, Profotova B, Nohynkova E, et al. Glycogen phosphorylase in Acanthamoeba spp.: determining the role of the enzyme during the encystment process using RNA interference. Eukaryot Cell. 2008;7:509-17 pubmed publisher
    b>Acanthamoeba infections are difficult to treat due to often late diagnosis and the lack of effective and specific therapeutic agents...
  36. Ozkoc S, Tuncay S, Delibas S, Akisu C, Ozbek Z, Durak I, et al. Identification of Acanthamoeba genotype T4 and Paravahlkampfia sp. from two clinical samples. J Med Microbiol. 2008;57:392-6 pubmed publisher
    In this study, two free-living amoebae strains, Acanthamoeba genotype T4 and Paravahlkampfia sp., which were isolated from keratitis cases are presented...
  37. Akya A, Pointon A, Thomas C. Viability of Listeria monocytogenes in co-culture with Acanthamoeba spp. FEMS Microbiol Ecol. 2009;70:20-9 pubmed publisher
    ..To examine the viability of L. monocytogenes in interaction with Acanthamoeba spp...
  38. Kandori M, Inoue T, Shimabukuro M, Hayashi H, Hori Y, Maeda N, et al. Four cases of Acanthamoeba keratitis treated with phototherapeutic keratectomy. Cornea. 2010;29:1199-202 pubmed publisher
    To report 4 cases of Acanthamoeba keratitis treated with excimer laser phototherapeutic keratectomy (PTK) and to discuss the clinical efficacy of this procedure...
  39. Khan N, Siddiqui R. Acanthamoeba affects the integrity of human brain microvascular endothelial cells and degrades the tight junction proteins. Int J Parasitol. 2009;39:1611-6 pubmed publisher
    Haematogenous spread is a key step in the development of Acanthamoeba granulomatous encephalitis, however it is not clear how circulating amoebae cross the blood-brain barrier to enter the CNS to produce disease...
  40. Ovrutsky A, Chan E, Kartalija M, Bai X, Jackson M, Gibbs S, et al. Cooccurrence of free-living amoebae and nontuberculous Mycobacteria in hospital water networks, and preferential growth of Mycobacterium avium in Acanthamoeba lenticulata. Appl Environ Microbiol. 2013;79:3185-92 pubmed publisher
    ..b>Acanthamoeba spp...
  41. Niyyati M, Lorenzo Morales J, Rezaie S, Rahimi F, Mohebali M, Maghsood A, et al. Genotyping of Acanthamoeba isolates from clinical and environmental specimens in Iran. Exp Parasitol. 2009;121:242-5 pubmed publisher
    In this study, 15 Acanthamoeba isolates from AK patients and 10 environmental samples (water, soil and animal-origin samples) were classified at the genotype level based on the sequence analysis of the Diagnostic Fragment 3 (DF3) of ..
  42. Derda M, Hadaś E. [Use of the presence of cellulose in cellular wall of Acanthamoeba cysts for diagnostic purposes]. Wiad Parazytol. 2009;55:47-51 pubmed
    Species identification within the genus Acanthamoeba is based predominantly on morphological and biochemical features. It is labor-intensive and requires cloning and axenization...
  43. Sandström G, Saeed A, Abd H. Acanthamoeba polyphaga is a possible host for Vibrio cholerae in aquatic environments. Exp Parasitol. 2010;126:65-8 pubmed publisher
    b>Acanthamoeba is a genus of free-living amoebae found to be able to host many bacterial species living in the environment. Acanthamoebae and Vibrio cholerae are found in the aquatic environments of cholera endemic areas...
  44. Mrva M, Garajova M, Lukac M, Ondriska F. Weak cytotoxic activity of miltefosine against clinical isolates of Acanthamoeba spp. J Parasitol. 2011;97:538-40 pubmed publisher
    ..drug active in vitro against various protozoan parasites, and recently used for the treatment of disseminated Acanthamoeba infection...
  45. Kawaguchi K, Matsuo J, Osaki T, Kamiya S, Yamaguchi H. Prevalence of Helicobacter and Acanthamoeba in natural environment. Lett Appl Microbiol. 2009;48:465-71 pubmed publisher
    We examined whether the presence of Helicobacter is related to that of Acanthamoeba in river and soil environments. The samples (river n = 51, soil n = 75) were collected in Sapporo City, Japan...
  46. Mortazavi P, Goldsworthy G, Kirk R, Khan N. Acanthamoeba produces disseminated infection in locusts and traverses the locust blood-brain barrier to invade the central nervous system. BMC Microbiol. 2010;10:186 pubmed publisher
    Many aspects of Acanthamoeba granulomatous encephalitis remain poorly understood, including host susceptibility and chronic colonization which represent important features of the spectrum of host-pathogen interactions...
  47. Visvesvara G, Moura H, Schuster F. Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunol Med Microbiol. 2007;50:1-26 pubmed
    ..free-living amoebae that exist in nature, members of only four genera have an association with human disease: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri and Sappinia diploidea. Acanthamoeba spp. and B...
  48. Acharya N, Lietman T, Margolis T. Parasites on the rise: a new epidemic of Acanthamoeba keratitis. Am J Ophthalmol. 2007;144:292-3 pubmed
  49. Moon E, Chung D, Hong Y, Kong H. Characterization of a serine proteinase mediating encystation of Acanthamoeba. Eukaryot Cell. 2008;7:1513-7 pubmed publisher
    Members of the genus Acanthamoeba, amphizoic protozoan parasites, are causative agents of granulomatous amoebic encephalitis and amoebic keratitis...
  50. Panjwani N. Pathogenesis of acanthamoeba keratitis. Ocul Surf. 2010;8:70-9 pubmed
    b>Acanthamoeba keratitis (AK) is a serious infection of the cornea. At present, diagnosis of the disease is not straightforward and treatment is very demanding...
  51. Yera H, Zamfir O, Bourcier T, Ancelle T, Batellier L, Dupouy Camet J, et al. Comparison of PCR, microscopic examination and culture for the early diagnosis and characterization of Acanthamoeba isolates from ocular infections. Eur J Clin Microbiol Infect Dis. 2007;26:221-4 pubmed
    ..examination and culture of corneal samples were compared as methods of confirming the clinical diagnosis of Acanthamoeba keratitis, a serious ocular infection that is difficult to diagnose and threatens eyesight...
  52. Joslin C, Tu E, Shoff M, Booton G, Fuerst P, McMahon T, et al. The association of contact lens solution use and Acanthamoeba keratitis. Am J Ophthalmol. 2007;144:169-180 pubmed
    To investigate Acanthamoeba keratitis (AK) risk factors. Diagnosis of AK, a rare but serious corneal infection, has recently increased significantly at the University of Illinois at Chicago (UIC) Cornea Service.
  53. Alsam S, Jeong S, Dudley R, Khan N. Role of human tear fluid in Acanthamoeba interactions with the human corneal epithelial cells. Int J Med Microbiol. 2008;298:329-36 pubmed
    b>Acanthamoeba keratitis is a painful and progressive sight-threatening infection. However, the precise mechanisms associated with the pathogenesis and pathophysiology of Acanthamoeba keratitis remain incompletely understood...
  54. Sriram R, Shoff M, Booton G, Fuerst P, Visvesvara G. Survival of Acanthamoeba cysts after desiccation for more than 20 years. J Clin Microbiol. 2008;46:4045-8 pubmed publisher
    b>Acanthamoeba is a free-living ameba that is found throughout the world and that causes encephalitis, keratitis, and cutaneous infections in humans. It has two stages in its life cycle: a trophic stage and a resistant cyst stage...
  55. Takaoka Sugihara N, Yamagami S, Yokoo S, Matsubara M, Yagita K. Cytopathic effect of Acanthamoeba on human corneal fibroblasts. Mol Vis. 2012;18:2221-8 pubmed
    b>Acanthamoeba keratitis is associated with keratocyte depletion in humans. We investigated how Acanthamoebae isolated from corneas affected by Acanthamoeba keratitis interacted with human corneal stromal cells in vitro...
  56. Gupta T, Fine Coulson K, Karls R, Gauthier D, Quinn F. Internalization of Mycobacterium shottsii and Mycobacterium pseudoshottsii by Acanthamoeba polyphaga. Can J Microbiol. 2013;59:570-6 pubmed publisher
    ..the trophozoite stage and reside in large numbers within the cytosol of the cyst of the free-living amoeba Acanthamoeba polyphaga. Results from the present study showed that M. shottsii, M. pseudoshottsii, and M...
  57. Niyyati M, Lorenzo Morales J, Rahimi F, Motevalli Haghi A, Martin Navarro C, Farnia S, et al. Isolation and genotyping of potentially pathogenic Acanthamoeba strains from dust sources in Iran. Trans R Soc Trop Med Hyg. 2009;103:425-7 pubmed publisher
    Opportunistic free-living amoebae of the Acanthamoeba genus present genotypes/strains that are causative agents of Acanthamoeba keratitis (AK), with genotype T4 related to most AK cases worldwide...
  58. Walochnik J, Obwaller A, Gruber F, Mildner M, Tschachler E, Suchomel M, et al. Anti-acanthamoeba efficacy and toxicity of miltefosine in an organotypic skin equivalent. J Antimicrob Chemother. 2009;64:539-45 pubmed publisher
    Acanthamoebae can cause infections of several organs, including eye, skin, lung and brain. Except for Acanthamoeba keratitis, these infections are linked to immunodeficiency...
  59. Kandori M, Inoue T, Takamatsu F, Kojima Y, Hori Y, Maeda N, et al. Two cases of acanthamoeba keratitis diagnosed only by real-time polymerase chain reaction. Cornea. 2010;29:228-31 pubmed publisher
    To report 2 cases of Acanthamoeba keratitis whose causative pathogen was detected only by real-time polymerase chain reaction (PCR).
  60. Ge Z, Qing Y, Zicheng S, Shiying S. Rapid and sensitive diagnosis of Acanthamoeba keratitis by loop-mediated isothermal amplification. Clin Microbiol Infect. 2013;19:1042-8 pubmed publisher
    A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of Acanthamoeba. The sensitivity of the LAMP assay was tested using different copies of positive DNA...
  61. Abergel C, Rudinger Thirion J, Giege R, Claverie J. Virus-encoded aminoacyl-tRNA synthetases: structural and functional characterization of mimivirus TyrRS and MetRS. J Virol. 2007;81:12406-17 pubmed
  62. Yagi S, Schuster F, Visvesvara G. Demonstration of Balamuthia and Acanthamoeba mitochondrial DNA in sectioned archival brain and other tissues by the polymerase chain reaction. Parasitol Res. 2008;102:491-7 pubmed
    ..encephalitis (GAE) is a usually fatal disease caused by the free-living amoebae Balamuthia mandrillaris and Acanthamoeba spp...
  63. Alizadeh H, Neelam S, Niederkorn J. Effect of immunization with the mannose-induced Acanthamoeba protein and Acanthamoeba plasminogen activator in mitigating Acanthamoeba keratitis. Invest Ophthalmol Vis Sci. 2007;48:5597-604 pubmed
    The mannose-induced cytopathic protein (MIP-133) and Acanthamoeba plasminogen activator (aPA) play key roles in the pathogenesis of Acanthamoeba keratitis by inducing a cytopathic effect on the corneal epithelial and stromal cells and by ..
  64. Xuan Y, Chung B, Hong Y, Kong H, Hahn T, Chung D. Keratitis by Acanthamoeba triangularis: report of cases and characterization of isolates. Korean J Parasitol. 2008;46:157-64 pubmed publisher
    Three Acanthamoeba isolates (KA/E9, KA/E17, and KA/E23) from patients with keratitis were identified as Acanthamoeba triangularis by analysis of their molecular characteristics, a species not previously recognized to be a corneal pathogen...
  65. Ebrahimi K, Green W, Grebe R, Jun A. Acanthamoeba sclerokeratitis. Graefes Arch Clin Exp Ophthalmol. 2009;247:283-6 pubmed publisher
    b>Acanthamoeba scleritis is an uncommon but severe complication of acanthamoeba keratitis. We report the clinical and histopathologic features of a patient with acanthamoeba sclerokeratitis.
  66. Claverie J, Abergel C, Ogata H. Mimivirus. Curr Top Microbiol Immunol. 2009;328:89-121 pubmed
    b>Acanthamoeba polyphaga Mimivirus, the first representative and prototype member of the Mimiviridae, is the latest addition to the menagerie of lesser-known big DNA viruses...
  67. Choi S, Cho M, Ahn S, Lee J, Lee J, Kim D, et al. Endosymbionts of Acanthamoeba isolated from domestic tap water in Korea. Korean J Parasitol. 2009;47:337-44 pubmed publisher
    In a previous study, we reported our discovery of Acanthamoeba contamination in domestic tap water; in that study, we determined that some Acanthamoeba strains harbor endosymbiotic bacteria, via our molecular characterization by ..
  68. Nagyová V, Nagy A, Timko J. Morphological, physiological and molecular biological characterisation of isolates from first cases of Acanthamoeba keratitis in Slovakia. Parasitol Res. 2010;106:861-72 pubmed publisher
    b>Acanthamoeba keratitis (AK) is a relatively rare disease worldwide. Over the past 10 years, five cases of AK were reported in Slovakia...
  69. Visvesvara G. Amebic meningoencephalitides and keratitis: challenges in diagnosis and treatment. Curr Opin Infect Dis. 2010;23:590-4 pubmed publisher
    b>Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri, although free-living amebae, also cause devastating diseases in humans leading to death. Acanthamoeba spp. and B...
  70. Pan H, Wu X. Hypoxia attenuates inflammatory mediators production induced by Acanthamoeba via Toll-like receptor 4 signaling in human corneal epithelial cells. Biochem Biophys Res Commun. 2012;420:685-91 pubmed publisher
    b>Acanthamoeba keratitis (AK) is a vision-threatening corneal infection that is intimately associated with contact lens use which leads to hypoxic conditions on the corneal surface...
  71. Boughalmi M, Saadi H, Pagnier I, Colson P, Fournous G, Raoult D, et al. High-throughput isolation of giant viruses of the Mimiviridae and Marseilleviridae families in the Tunisian environment. Environ Microbiol. 2013;15:2000-7 pubmed publisher
    ..Thus, few environments have been exhaustively investigated to date, although the ubiquitous distribution of the Acanthamoeba sp. suggests that these viruses could also be ubiquitous...
  72. Zauberman N, Mutsafi Y, Halevy D, Shimoni E, Klein E, Xiao C, et al. Distinct DNA exit and packaging portals in the virus Acanthamoeba polyphaga mimivirus. PLoS Biol. 2008;6:e114 pubmed publisher
    ..Here we present an exception to this paradigm by demonstrating that genome delivery and packaging in the virus Acanthamoeba polyphaga mimivirus occur through two distinct portals...
  73. Claverie J, Abergel C. Mimivirus and its virophage. Annu Rev Genet. 2009;43:49-66 pubmed publisher
    Mimivirus, a virus infecting amoebae of the acanthamoeba genus, is the prototype member of the Mimiviridae, the latest addition to the family of the nucleocytoplasmic large DNA viruses, already including the Poxviridae, the Iridoviridae, ..
  74. Ben Salah I, Drancourt M. Surviving within the amoebal exocyst: the Mycobacterium avium complex paradigm. BMC Microbiol. 2010;10:99 pubmed publisher
    ..Mycobacterium timonense and Mycobacterium bouchedurhonense were co-cultivated with the free-living amoeba Acanthamoeba polyphaga strain Linc-AP1...
  75. Cateau E, Verdon J, Fernandez B, Hechard Y, Rodier M. Acanthamoeba sp. promotes the survival and growth of Acinetobacter baumanii. FEMS Microbiol Lett. 2011;319:19-25 pubmed publisher
    ..In this paper, we have studied potential relationships between A. baumanii and Acanthamoeba species. Two strains of A...
  76. Colson P, Yutin N, Shabalina S, Robert C, Fournous G, La Scola B, et al. Viruses with more than 1,000 genes: Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, and reannotation of Mimivirus genes. Genome Biol Evol. 2011;3:737-42 pubmed publisher
    The genome sequence of the Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, is reported. With 1,191,693 nt in length and 1,023 predicted protein-coding genes, the Mamavirus has the largest genome among the known viruses...
  77. Yoosuf N, Yutin N, Colson P, Shabalina S, Pagnier I, Robert C, et al. Related giant viruses in distant locations and different habitats: Acanthamoeba polyphaga moumouvirus represents a third lineage of the Mimiviridae that is close to the megavirus lineage. Genome Biol Evol. 2012;4:1324-30 pubmed publisher
    The 1,021,348 base pair genome sequence of the Acanthamoeba polyphaga moumouvirus, a new member of the Mimiviridae family infecting Acanthamoeba polyphaga, is reported...
  78. Thompson P, Kowalski R, Shanks R, Gordon Y. Validation of real-time PCR for laboratory diagnosis of Acanthamoeba keratitis. J Clin Microbiol. 2008;46:3232-6 pubmed publisher
    Confirmation of Acanthamoeba keratitis by laboratory diagnosis is the first step in the treatment of this vision-threatening disease...
  79. Ren M, Gao L, Wu X. TLR4: the receptor bridging Acanthamoeba challenge and intracellular inflammatory responses in human corneal cell lines. Immunol Cell Biol. 2010;88:529-36 pubmed publisher
    b>Acanthamoeba keratitis (AK) is a painful, vision-threatening infection caused by pathogenic strains of the protozoan, Acanthamoeba...
  80. Heaselgrave W, Andrew P, Kilvington S. Acidified nitrite enhances hydrogen peroxide disinfection of Acanthamoeba, bacteria and fungi. J Antimicrob Chemother. 2010;65:1207-14 pubmed publisher
    ..In this study, the efficacy of hydrogen peroxide (H2O2) and acidified nitrite (NaNO2) alone and in combination was compared against a variety of bacteria, fungi and protozoa...
  81. Lorenzo Morales J, Morcillo Laiz R, Martín Navarro C, Lopez Velez R, López Arencibia A, Arnalich Montiel F, et al. Acanthamoeba keratitis due to genotype T11 in a rigid gas permeable contact lens wearer in Spain. Cont Lens Anterior Eye. 2011;34:83-6 pubmed publisher
    ..The patient did not respond to this treatment and six weeks later, corneal scrapings were positive for Acanthamoeba genotype T11. She was then treated with chlorhexidine 0.02%, propamidine 0...
  82. Arslan D, Legendre M, Seltzer V, Abergel C, Claverie J. Distant Mimivirus relative with a larger genome highlights the fundamental features of Megaviridae. Proc Natl Acad Sci U S A. 2011;108:17486-91 pubmed publisher
    Mimivirus, a DNA virus infecting acanthamoeba, was for a long time the largest known virus both in terms of particle size and gene content...
  83. Ondarza R. Drug targets from human pathogenic amoebas: Entamoeba histolytica, Acanthamoeba polyphaga and Naegleria fowleri. Infect Disord Drug Targets. 2007;7:266-80 pubmed
    ..of drug targets in several species of human pathogenic parasites, mainly the amoebas Entamoeba histolytica, Acanthamoeba polyphaga and Naegleria fowleri...
  84. Siddiqui R, Emes R, Elsheikha H, Khan N. Area 51: How do Acanthamoeba invade the central nervous system?. Trends Parasitol. 2011;27:185-9 pubmed publisher
    b>Acanthamoeba granulomatous encephalitis generally develops as a result of haematogenous spread, but it is unclear how circulating amoebae enter the central nervous system (CNS) and cause inflammation...
  85. Bouheraoua N, Gaujoux T, Goldschmidt P, Chaumeil C, Laroche L, Borderie V. Prognostic factors associated with the need for surgical treatments in acanthamoeba keratitis. Cornea. 2013;32:130-6 pubmed publisher
    The objective of this study was to assess the factors associated with anatomical and visual outcomes in patients presenting with Acanthamoeba keratitis (AK).
  86. Voyatzis G, McElvanney A. Bilateral Acanthamoeba keratitis in an experienced two-weekly disposable contact lens wearer. Eye Contact Lens. 2007;33:201-2 pubmed
    To present a case of bilateral Acanthamoeba keratitis in a patient who stored both contact lenses in the same storage case.
  87. Tu E, Joslin C, Sugar J, Booton G, Shoff M, Fuerst P. The relative value of confocal microscopy and superficial corneal scrapings in the diagnosis of Acanthamoeba keratitis. Cornea. 2008;27:764-72 pubmed publisher
    To compare the relative diagnostic value of confocal microscopy and superficial corneal cultures in the diagnosis of Acanthamoeba keratitis by using clinical and microbiologic definitions of disease.
  88. Kilvington S, Lonnen J. A comparison of regimen methods for the removal and inactivation of bacteria, fungi and Acanthamoeba from two types of silicone hydrogel lenses. Cont Lens Anterior Eye. 2009;32:73-7 pubmed publisher
    ..To compare the antimicrobial efficacy of commercial contact lens solutions when used according to the manufacturers' recommended regimens with two types of silicone hydrogel lenses...
  89. Nuprasert W, Putaporntip C, Pariyakanok L, Jongwutiwes S. Identification of a novel t17 genotype of acanthamoeba from environmental isolates and t10 genotype causing keratitis in Thailand. J Clin Microbiol. 2010;48:4636-40 pubmed publisher
    We analyzed the nuclear small-subunit rRNA genes of Acanthamoeba isolates from freshwater sources (n=16) and from patients (n=6) in Bangkok and surrounding areas...
  90. Siddiqui R, Khan N. Acanthamoeba is an evolutionary ancestor of macrophages: a myth or reality?. Exp Parasitol. 2012;130:95-7 pubmed publisher
    ..to capture prey by phagocytosis and interactions with microbial pathogens, here we pose the question whether Acanthamoeba and macrophages are evolutionary related...
  91. Casson N, Posfay Barbe K, Gervaix A, Greub G. New diagnostic real-time PCR for specific detection of Parachlamydia acanthamoebae DNA in clinical samples. J Clin Microbiol. 2008;46:1491-3 pubmed publisher
    ..When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis...
  92. Patel A, Hammersmith K. Contact lens-related microbial keratitis: recent outbreaks. Curr Opin Ophthalmol. 2008;19:302-6 pubmed publisher
    ..This paper reviews the literature generated on atypical contact lens-related infections and their relationship with particular contact lens solutions since 2005...