tetrahymena thermophila

Summary

Summary: A species of ciliate protozoa used in genetic and cytological research.

Top Publications

  1. Dou Y, Song X, Liu Y, Gorovsky M. The H1 phosphorylation state regulates expression of CDC2 and other genes in response to starvation in Tetrahymena thermophila. Mol Cell Biol. 2005;25:3914-22 pubmed
    In Tetrahymena thermophila, highly phosphorylated histone H1 of growing cells becomes partially dephosphorylated when cells are starved in preparation for conjugation...
  2. Chen Y, Fender J, Legassie J, Jarstfer M, Bryan T, Varani G. Structure of stem-loop IV of Tetrahymena telomerase RNA. EMBO J. 2006;25:3156-66 pubmed
    ..The NMR structure of Tetrahymena thermophila stem-loop IV shows a highly structured distal stem-loop linked to a conformationally flexible template-..
  3. Ross I, Clarissa C, Giddings T, Winey M. ?-tubulin is essential in Tetrahymena thermophila for the assembly and stability of basal bodies. J Cell Sci. 2013;126:3441-51 pubmed publisher
    ..We have identified the ?-tubulin gene in Tetrahymena thermophila and detected the protein, through fluorescence of a tagged allele, to basal bodies...
  4. Iwamoto M, Sugai T, Nakaoka Y. Cell division induced by mechanical stimulation in starved Tetrahymena thermophila: cell cycle without synthesis of macronuclear DNA. Cell Biol Int. 2004;28:503-9 pubmed
    Starved Tetrahymena thermophila cells underwent synchronous cell division 2 h after a mechanical stimulation...
  5. Malone C, Anderson A, Motl J, Rexer C, Chalker D. Germ line transcripts are processed by a Dicer-like protein that is essential for developmentally programmed genome rearrangements of Tetrahymena thermophila. Mol Cell Biol. 2005;25:9151-64 pubmed
    ..promote the elimination of nearly 15 Mbp of DNA from the developing somatic genome are generated during Tetrahymena thermophila conjugation...
  6. Cheng C, Vogt A, Mochizuki K, Yao M. A domesticated piggyBac transposase plays key roles in heterochromatin dynamics and DNA cleavage during programmed DNA deletion in Tetrahymena thermophila. Mol Biol Cell. 2010;21:1753-62 pubmed publisher
    ..These findings suggest that Tpb2p plays a key role in the assembly of specialized DNA elimination chromatin architectures and is likely responsible for the DNA cleavage step of programmed DNA deletion. ..
  7. Klinge S, Voigts Hoffmann F, Leibundgut M, Arpagaus S, Ban N. Crystal structure of the eukaryotic 60S ribosomal subunit in complex with initiation factor 6. Science. 2011;334:941-8 pubmed publisher
    ..We present the structure of the 60S ribosomal subunit from Tetrahymena thermophila in complex with eukaryotic initiation factor 6 (eIF6), cocrystallized with the antibiotic cycloheximide (..
  8. Herrmann L, Erkelenz M, Aldag I, Tiedtke A, Hartmann M. Biochemical and molecular characterisation of Tetrahymena thermophila extracellular cysteine proteases. BMC Microbiol. 2006;6:19 pubmed
    ..Over the last decades molecular biologic techniques have been developed to alter the genome and proteome of Tetrahymena thermophila thereby providing the basis for recombinant protein expression including functional human enzymes...
  9. Richards R, Wu H, Trantirek L, O Connor C, Collins K, Feigon J. Structural study of elements of Tetrahymena telomerase RNA stem-loop IV domain important for function. RNA. 2006;12:1475-85 pubmed
    ..The results suggest that during holoenzyme assembly the protein p65 recognizes a bend in stem IV, and this binding to central stem IV helps to position the structured loop IV for interaction with TERT and other region(s) of TER. ..

More Information

Publications104 found, 100 shown here

  1. Stone M, Mihalusova M, O Connor C, Prathapam R, Collins K, Zhuang X. Stepwise protein-mediated RNA folding directs assembly of telomerase ribonucleoprotein. Nature. 2007;446:458-61 pubmed
    ..These results identify the RNA folding pathway during telomerase biogenesis and define the mechanism of action for an essential telomerase holoenzyme protein. ..
  2. Mochizuki K. High efficiency transformation of Tetrahymena using a codon-optimized neomycin resistance gene. Gene. 2008;425:79-83 pubmed publisher
    b>Tetrahymena thermophila is a useful model for the study of eukaryotic biology. A neomycin resistance gene (neo) has been developed that was optimized for the codon usage of T. thermophila...
  3. Blinova I, Ivask A, Heinlaan M, Mortimer M, Kahru A. Ecotoxicity of nanoparticles of CuO and ZnO in natural water. Environ Pollut. 2010;158:41-7 pubmed publisher
    ..AFW) and in natural waters to crustaceans Daphnia magna and Thamnocephalus platyurus and protozoan Tetrahymena thermophila was compared...
  4. Brown J, Fine N, Pandiyan G, Thazhath R, Gaertig J. Hypoxia regulates assembly of cilia in suppressors of Tetrahymena lacking an intraflagellar transport subunit gene. Mol Biol Cell. 2003;14:3192-207 pubmed
    We cloned a Tetrahymena thermophila gene, IFT52, encoding a homolog of the Chlamydomonas intraflagellar transport protein, IFT52...
  5. Witkin K, Collins K. Holoenzyme proteins required for the physiological assembly and activity of telomerase. Genes Dev. 2004;18:1107-18 pubmed
    ..These findings demonstrate that telomerase holoenzyme proteins other than TERT play critical roles in RNP biogenesis and function. ..
  6. Cui B, Liu Y, Gorovsky M. Deposition and function of histone H3 variants in Tetrahymena thermophila. Mol Cell Biol. 2006;26:7719-30 pubmed publisher
    ..Minor H3s are also required to produce viable sexual progeny and play an unexpected role in the germ line micronuclei late in conjugation that is unrelated to transcription...
  7. Eisen J, Coyne R, Wu M, Wu D, Thiagarajan M, Wortman J, et al. Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote. PLoS Biol. 2006;4:e286 pubmed
    The ciliate Tetrahymena thermophila is a model organism for molecular and cellular biology. Like other ciliates, this species has separate germline and soma functions that are embodied by distinct nuclei within a single cell...
  8. Pearson C, Giddings T, Winey M. Basal body components exhibit differential protein dynamics during nascent basal body assembly. Mol Biol Cell. 2009;20:904-14 pubmed publisher
    ..High-resolution light and electron microscopy studies were performed in Tetrahymena thermophila to determine how proteins assemble into the structure...
  9. Baran N, Haviv Y, Paul B, Manor H. Studies on the minimal lengths required for DNA primers to be extended by the Tetrahymena telomerase: implications for primer positioning by the enzyme. Nucleic Acids Res. 2002;30:5570-8 pubmed
  10. Mochizuki K, Gorovsky M. Small RNAs in genome rearrangement in Tetrahymena. Curr Opin Genet Dev. 2004;14:181-7 pubmed
    ..These findings contribute to a new paradigm of chromatin biology: regulation of heterochromatin formation by RNAi-related mechanisms in eukaryotes. ..
  11. O Connor C, Collins K. A novel RNA binding domain in tetrahymena telomerase p65 initiates hierarchical assembly of telomerase holoenzyme. Mol Cell Biol. 2006;26:2029-36 pubmed
    ..These findings elucidate an extensive network of p65-TER recognition specificity and define a novel p65 RNA binding domain that initiates telomerase holoenyzme biogenesis. ..
  12. Hamilton E, Williamson S, Dunn S, Merriam V, Lin C, Vong L, et al. The highly conserved family of Tetrahymena thermophila chromosome breakage elements contains an invariant 10-base-pair core. Eukaryot Cell. 2006;5:771-80 pubmed
    As a typical ciliate, Tetrahymena thermophila is a unicellular eukaryote that exhibits nuclear dimorphism: each cell contains a diploid, germ line micronucleus (MICN) and a polyploid, somatic macronucleus (MACN)...
  13. Cervantes M, Xi X, Vermaak D, Yao M, Malik H. The CNA1 histone of the ciliate Tetrahymena thermophila is essential for chromosome segregation in the germline micronucleus. Mol Biol Cell. 2006;17:485-97 pubmed
    ..To study ciliate chromosome segregation, we have identified the centromeric histone gene in the Tetrahymena thermophila genome (CNA1)...
  14. Bednenko J, Noto T, DeSouza L, Siu K, Pearlman R, Mochizuki K, et al. Two GW repeat proteins interact with Tetrahymena thermophila argonaute and promote genome rearrangement. Mol Cell Biol. 2009;29:5020-30 pubmed publisher
    In conjugating Tetrahymena thermophila, massive DNA elimination occurs upon the development of the new somatic genome from the germ line genome...
  15. Becker B, Rüsing M. Structure of N-glycosidic carbohydrates of secretory proteins of Tetrahymena thermophila. J Eukaryot Microbiol. 2003;50:235-9 pubmed
    ..Further analysis using the FACE technology revealed the presence of four different N-glycosidic structures differing only in the number of mannose residues attached to the core chitobiose unit. ..
  16. Saito Nakano Y, Nakahara T, Nakano K, Nozaki T, Numata O. Marked amplification and diversification of products of ras genes from rat brain, Rab GTPases, in the ciliates Tetrahymena thermophila and Paramecium tetraurelia. J Eukaryot Microbiol. 2010;57:389-99 pubmed publisher
    ..Rab encoded in the genome correlates with multicellularity; however, we found that unicellular ciliates Tetrahymena thermophila (Tt) and Paramecium tetraurelia (Pt) possess many more Rab genes in their genome than the 64 HsRab genes ..
  17. Cowan A, Bowman G, Edwards K, Emerson J, Turkewitz A. Genetic, genomic, and functional analysis of the granule lattice proteins in Tetrahymena secretory granules. Mol Biol Cell. 2005;16:4046-60 pubmed
    ..In ciliates such as Tetrahymena thermophila, the resulting crystals function as projectiles, expanding upon exocytosis...
  18. Stover N, Krieger C, Binkley G, Dong Q, Fisk D, Nash R, et al. Tetrahymena Genome Database (TGD): a new genomic resource for Tetrahymena thermophila research. Nucleic Acids Res. 2006;34:D500-3 pubmed
    ..ciliate.org) for researchers studying the model ciliate organism Tetrahymena thermophila. Employing the underlying database structure and programming of the Saccharomyces Genome Database, the ..
  19. Vonderfecht T, Stemm Wolf A, Hendershott M, Giddings T, Meehl J, Winey M. The two domains of centrin have distinct basal body functions in Tetrahymena. Mol Biol Cell. 2011;22:2221-34 pubmed publisher
    ..The results also show that the two domains of Cen1 are able to bind Ca(2+) and that perturbation of Ca(2+) binding affects Cen1 function. In all, the data suggest that the two domains of Cen1 have distinct functions. ..
  20. Nusblat A, Muñoz L, Valcarce G, Nudel C. Characterization and properties of cholesterol desaturases from the ciliate Tetrahymena thermophila. J Eukaryot Microbiol. 2005;52:61-7 pubmed
    Live Tetrahymena thermophila transforms exogenous cholesterol into 7,22-bis, dehydrocholesterol (DHC) by desaturation at positions C7(8) and C22(23) of the cholesterol moiety...
  21. Madinger C, Collins K, Fields L, Taron C, Benner J. Constitutive secretion in Tetrahymena thermophila. Eukaryot Cell. 2010;9:674-81 pubmed publisher
    The growth, survival, and life cycle progression of the freshwater ciliated protozoan Tetrahymena thermophila are responsive to protein signals thought to be released by constitutive secretion...
  22. Brunk C, Lee L, Tran A, Li J. Complete sequence of the mitochondrial genome of Tetrahymena thermophila and comparative methods for identifying highly divergent genes. Nucleic Acids Res. 2003;31:1673-82 pubmed
    The complete sequence of the mitochondrial genome of Tetrahymena thermophila has been determined and compared with the mitochondrial genome of Tetrahymena pyriformis. The sequence similarity clearly indicates homology of the entire T...
  23. Malone C, Falkowska K, Li A, Galanti S, Kanuru R, LaMont E, et al. Nucleus-specific importin alpha proteins and nucleoporins regulate protein import and nuclear division in the binucleate Tetrahymena thermophila. Eukaryot Cell. 2008;7:1487-99 pubmed publisher
    The ciliate Tetrahymena thermophila, having both germ line micronuclei and somatic macronuclei, must possess a specialized nucleocytoplasmic transport system to import proteins into the correct nucleus...
  24. Chalker D. Dynamic nuclear reorganization during genome remodeling of Tetrahymena. Biochim Biophys Acta. 2008;1783:2130-6 pubmed publisher
    The single-celled ciliate Tetrahymena thermophila possesses two versions of its genome, one germline, one somatic, contained within functionally distinct nuclei (called the micronucleus and macronucleus, respectively)...
  25. Orias E, Cervantes M, Hamilton E. Tetrahymena thermophila, a unicellular eukaryote with separate germline and somatic genomes. Res Microbiol. 2011;162:578-86 pubmed publisher
    b>Tetrahymena thermophila is a ciliate--a unicellular eukaryote. Remarkably, every cell maintains differentiated germline and somatic genomes: one silent, the other expressed...
  26. Liu Y, Song X, Gorovsky M, Karrer K. Elimination of foreign DNA during somatic differentiation in Tetrahymena thermophila shows position effect and is dosage dependent. Eukaryot Cell. 2005;4:421-31 pubmed
    In the ciliate Tetrahymena thermophila, approximately 15% of the germ line micronuclear DNA sequences are eliminated during formation of the somatic macronucleus...
  27. Ostrowski S, Van Bell C, Winograd N, Ewing A. Mass spectrometric imaging of highly curved membranes during Tetrahymena mating. Science. 2004;305:71-3 pubmed
    ..This evidence suggests that biological fusion involves and might in fact be driven by a heterogeneous redistribution of lipids at the fusion site. ..
  28. Morrison T, Yakisich J, Cassidy Hanley D, Kapler G. TIF1 Represses rDNA replication initiation, but promotes normal S phase progression and chromosome transmission in Tetrahymena. Mol Biol Cell. 2005;16:2624-35 pubmed
    The non-ORC protein, TIF1, recognizes sequences in the Tetrahymena thermophila ribosomal DNA (rDNA) minichromosome that are required for origin activation...
  29. Rahaman A, Elde N, Turkewitz A. A dynamin-related protein required for nuclear remodeling in Tetrahymena. Curr Biol. 2008;18:1227-33 pubmed publisher
    ..The eight DRPs in the ciliate Tetrahymena thermophila might contribute to aspects of ciliate complexity...
  30. Nusblat A, Bright L, Turkewitz A. Conservation and innovation in Tetrahymena membrane traffic: proteins, lipids, and compartments. Methods Cell Biol. 2012;109:141-75 pubmed publisher
    The past decade has seen a significant expansion in our understanding of membrane traffic in Tetrahymena thermophila, facilitated by the development of new experimental tools and by the availability of the macronuclear genome sequence...
  31. Cervantes M, Hamilton E, Xiong J, Lawson M, Yuan D, Hadjithomas M, et al. Selecting one of several mating types through gene segment joining and deletion in Tetrahymena thermophila. PLoS Biol. 2013;11:e1001518 pubmed publisher
    The unicellular eukaryote Tetrahymena thermophila has seven mating types. Cells can mate only when they recognize cells of a different mating type as non-self. As a ciliate, Tetrahymena separates its germline and soma into two nuclei...
  32. Miller M, Collins K. Telomerase recognizes its template by using an adjacent RNA motif. Proc Natl Acad Sci U S A. 2002;99:6585-90 pubmed
    ..Here, using circularly permuted variants of Tetrahymena thermophila telomerase RNA, we identify the features that allow recognition of the template region within the RNA...
  33. Mochizuki K, Fine N, Fujisawa T, Gorovsky M. Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in tetrahymena. Cell. 2002;110:689-99 pubmed
    ..We identified a gene, TWI1, in Tetrahymena thermophila that is homologous to piwi and is required for DNA elimination...
  34. Miao W, Yu T, Orias E, Wan M, Fu C. Identification of differentially expressed genes in Tetrahymena thermophila in response to dichlorodiphenyltrichloroethane (DDT) by suppression subtractive hybridization. Environ Microbiol. 2006;8:1122-9 pubmed
    ..The results show that Tetrahymena is a relevant and useful model organism for detecting DDT in the environment and for discovering biomarkers that can be used to develop specific bio-reporters at the molecular and genomic levels...
  35. Wilkes D, Rajagopalan V, Chan C, Kniazeva E, Wiedeman A, Asai D. Dynein light chain family in Tetrahymena thermophila. Cell Motil Cytoskeleton. 2007;64:82-96 pubmed
    ..We have searched the recently sequenced Tetrahymena thermophila macronuclear genome to describe the entire family of dynein light chains expressed in this organism...
  36. Yao M, Yao C, Halasz L, Fuller P, Rexer C, Wang S, et al. Identification of novel chromatin-associated proteins involved in programmed genome rearrangements in Tetrahymena. J Cell Sci. 2007;120:1978-89 pubmed
    ..differentiation of the developing somatic macronuclear genome from the germ line micronuclear genome of Tetrahymena thermophila. To identify genes encoding proteins likely to be involved in this process, we devised a cytological ..
  37. Lainhart W, Stolfa G, Koudelka G. Shiga toxin as a bacterial defense against a eukaryotic predator, Tetrahymena thermophila. J Bacteriol. 2009;191:5116-22 pubmed publisher
    ..We show here that Tetrahymena thermophila, a bacterivorous predator, is killed when cocultured with bacteria bearing a Shiga toxin (Stx)-encoding ..
  38. Uchida T, Takamoto K, He Q, Chance M, Brenowitz M. Multiple monovalent ion-dependent pathways for the folding of the L-21 Tetrahymena thermophila ribozyme. J Mol Biol. 2003;328:463-78 pubmed
    ..The Mg(2+)-dependent folding of the L-21 Sca 1 Tetrahymena thermophila ribozyme has been followed using this technique at an elevated concentration of monovalent ion (200 mM ..
  39. Selker E. Molecular biology. A self-help guide for a trim genome. Science. 2003;300:1517-8 pubmed
  40. Collins K, Gorovsky M. Tetrahymena thermophila. Curr Biol. 2005;15:R317-8 pubmed
  41. Kobayashi T, Endoh H. A possible role of mitochondria in the apoptotic-like programmed nuclear death of Tetrahymena thermophila. FEBS J. 2005;272:5378-87 pubmed
    ..Taking account of the present observations and the timing of autophagosome formation, we conclude that mitochondria might be involved in Tetrahymena PND, probably with the process of oligonucleosomal laddering...
  42. Fjerdingstad E, Schtickzelle N, Manhes P, Gutierrez A, Clobert J. Evolution of dispersal and life history strategies--Tetrahymena ciliates. BMC Evol Biol. 2007;7:133 pubmed
    ..Yet such studies remain scarce. Here we studied the ciliated protist Tetrahymena thermophila, a particularly interesting organism due to cells being able to transform into morphs differing ..
  43. Robinette E, Gulley K, Cassity K, King E, Nielsen A, Rozelle C, et al. A comparison of the polycation receptors of Paramecium tetraurelia and Tetrahymena thermophila. J Eukaryot Microbiol. 2008;55:86-90 pubmed publisher
    ..Polycation responses of both organisms are inhibited by neomycin sulfate. While PACAP is the most effective of the three chemorepellents in Tetrahymena, lysozyme is the most effective chemorepellent in Paramecium...
  44. Fu C, Xiong J, Miao W. Genome-wide identification and characterization of cytochrome P450 monooxygenase genes in the ciliate Tetrahymena thermophila. BMC Genomics. 2009;10:208 pubmed publisher
  45. Aldag I, Bockau U, Rossdorf J, Laarmann S, Raaben W, Herrmann L, et al. Expression, secretion and surface display of a human alkaline phosphatase by the ciliate Tetrahymena thermophila. BMC Biotechnol. 2011;11:11 pubmed publisher
    b>Tetrahymena thermophila possesses many attributes that render it an attractive host for the expression of recombinant proteins...
  46. Iwamoto M, Nakaoka Y. External GTP binding and induction of cell division in starved Tetrahymena thermophila. Eur J Cell Biol. 2002;81:517-24 pubmed
    ..we studied the surface localization of GTP binding sites and also its effects on the cell division of Tetrahymena thermophila. When a ribose-modified and fluorescent analog of GTP, 2'-(or -3')-O-trinitrophenyl (TNP)-GTP was added ..
  47. Haddad A, Bowman G, Turkewitz A. New class of cargo protein in Tetrahymena thermophila dense core secretory granules. Eukaryot Cell. 2002;1:583-93 pubmed
    ..Following exocytosis, the cores generally disassemble to diffuse in the cell environment. The ciliates Tetrahymena thermophila and Paramecium tetraurelia have been advanced as genetically manipulatable systems for studying ..
  48. Dayeh V, Lynn D, Bols N. Cytotoxicity of metals common in mining effluent to rainbow trout cell lines and to the ciliated protozoan, Tetrahymena thermophila. Toxicol In Vitro. 2005;19:399-410 pubmed
    ..samples for toxicity, two cell lines from rainbow trout, RTgill-W1 and RTL-W1, and the ciliate, Tetrahymena thermophila, were compared for their sensitivity to the toxicity of five metals that are commonly found in mining ..
  49. Stemm Wolf A, Morgan G, Giddings T, White E, Marchione R, McDonald H, et al. Basal body duplication and maintenance require one member of the Tetrahymena thermophila centrin gene family. Mol Biol Cell. 2005;16:3606-19 pubmed
    ..The ciliate Tetrahymena thermophila contains at least four centrin genes as determined by sequence homology, and these have distinct ..
  50. Richards R, Theimer C, Finger L, Feigon J. Structure of the Tetrahymena thermophila telomerase RNA helix II template boundary element. Nucleic Acids Res. 2006;34:816-25 pubmed
    ..Here, we report the solution structure of the TBE element (helix II) from Tetrahymena thermophila telomerase RNA...
  51. Theimer C, Feigon J. Structure and function of telomerase RNA. Curr Opin Struct Biol. 2006;16:307-18 pubmed
    ..The domains and interactions of the Tetrahymena thermophila telomerase holoenzyme RNA and protein components have been further characterized biochemically, and ..
  52. Rouda S, Skordalakes E. Structure of the RNA-binding domain of telomerase: implications for RNA recognition and binding. Structure. 2007;15:1403-12 pubmed
    ..Here, we present a high-resolution structure of TRBD from Tetrahymena thermophila. The nearly all-helical structure comprises a nucleic acid-binding fold suitable for TER binding...
  53. Dave D, Wloga D, Sharma N, Gaertig J. DYF-1 Is required for assembly of the axoneme in Tetrahymena thermophila. Eukaryot Cell. 2009;8:1397-406 pubmed publisher
    ..Our observations support the conserved function for DYF-1 in axoneme assembly or stability but also show that the consequences of loss of DYF-1 for axoneme segments are organism specific...
  54. Couvillion M, Lee S, Hogstad B, Malone C, Tonkin L, Sachidanandam R, et al. Sequence, biogenesis, and function of diverse small RNA classes bound to the Piwi family proteins of Tetrahymena thermophila. Genes Dev. 2009;23:2016-32 pubmed publisher
    ..The ciliate Tetrahymena thermophila encodes numerous PPD proteins exclusively of the Piwi clade...
  55. Salim H, Negritto M, Cavalcanti A. 1+1 = 3: a fusion of 2 enzymes in the methionine salvage pathway of Tetrahymena thermophila creates a trifunctional enzyme that catalyzes 3 steps in the pathway. PLoS Genet. 2009;5:e1000701 pubmed publisher
    ..a computational approach to identify the enzymes of the methionine salvage pathway in another eukaryote, Tetrahymena thermophila. In this organism, the pathway has two fused genes, MTNAK and MTNBD...
  56. Noto T, Kurth H, Kataoka K, Aronica L, DeSouza L, Siu K, et al. The Tetrahymena argonaute-binding protein Giw1p directs a mature argonaute-siRNA complex to the nucleus. Cell. 2010;140:692-703 pubmed publisher
    ..These results suggest that Giw1p senses the state of Twi1p-associated siRNAs and selectively transports the mature Twi1p-siRNA complex into the nucleus...
  57. Berman A, Gooding A, Cech T. Tetrahymena telomerase protein p65 induces conformational changes throughout telomerase RNA (TER) and rescues telomerase reverse transcriptase and TER assembly mutants. Mol Cell Biol. 2010;30:4965-76 pubmed publisher
    ..Second, p65 acts as a molecular buttress within the assembled RNP, mutually stabilizing TER and TERT in catalytically active conformations...
  58. Xiong J, Lu X, Zhou Z, Chang Y, Yuan D, Tian M, et al. Transcriptome analysis of the model protozoan, Tetrahymena thermophila, using Deep RNA sequencing. PLoS ONE. 2012;7:e30630 pubmed publisher
    The ciliated protozoan Tetrahymena thermophila is a well-studied single-celled eukaryote model organism for cellular and molecular biology. However, the lack of extensive T...
  59. Thazhath R, Liu C, Gaertig J. Polyglycylation domain of beta-tubulin maintains axonemal architecture and affects cytokinesis in Tetrahymena. Nat Cell Biol. 2002;4:256-9 pubmed
    ..In Tetrahymena thermophila, multiple sites of polyglycylation on alpha-tubulin are dispensable...
  60. Fillingham J, Chilcoat N, Turkewitz A, Orias E, Reith M, Pearlman R. Analysis of expressed sequence tags (ESTs) in the ciliated protozoan Tetrahymena thermophila. J Eukaryot Microbiol. 2002;49:99-107 pubmed
    ..of expressed sequence tag (EST) sequencing as a method of gene discovery in the ciliated protozoan Tetrahymena thermophila, we have sequenced either the 5' or 3' ends of 157 clones chosen at random from two cDNA libraries ..
  61. Taverna S, Coyne R, Allis C. Methylation of histone h3 at lysine 9 targets programmed DNA elimination in tetrahymena. Cell. 2002;110:701-11 pubmed
    ..These results extend the range of H3(Lys9)Me involvement in chromatin activities outside transcriptional regulation and also strengthen the link between heterochromatin formation and programmed DNA elimination...
  62. Shang Y, Li B, Gorovsky M. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol. 2002;158:1195-206 pubmed
    The gene (GTU1) encoding Tetrahymena thermophila gamma-tubulin was cloned and analyzed. GTU1 is a single-copy, essential gene encoding a conventional gamma-tubulin...
  63. Churkin A, Barash D. RNAmute: RNA secondary structure mutation analysis tool. BMC Bioinformatics. 2006;7:221 pubmed
  64. Weide T, Bockau U, Rave A, Herrmann L, Hartmann M. A recombinase system facilitates cloning of expression cassettes in the ciliate Tetrahymena thermophila. BMC Microbiol. 2007;7:12 pubmed
    b>Tetrahymena thermophila is one of the best characterized unicellular eukaryotes and its genome is sequenced in its entirety...
  65. Robart A, O Connor C, Collins K. Ciliate telomerase RNA loop IV nucleotides promote hierarchical RNP assembly and holoenzyme stability. RNA. 2010;16:563-71 pubmed publisher
    ..resolve the functional requirements for this motif of ciliate TER in physiological RNP context using the Tetrahymena thermophila p65-TER-TERT core RNP reconstituted in vitro and the holoenzyme reconstituted in vivo...
  66. Bright L, Kambesis N, Nelson S, Jeong B, Turkewitz A. Comprehensive analysis reveals dynamic and evolutionary plasticity of Rab GTPases and membrane traffic in Tetrahymena thermophila. PLoS Genet. 2010;6:e1001155 pubmed publisher
    ..Thus, characterizing entire gene families can provide insight into the evolutionary flexibility of fundamental cellular pathways...
  67. Kaczanowski A, Kiersnowska M. Inactivation of a macronuclear intra-S-phase checkpoint in Tetrahymena thermophila with caffeine affects the integrity of the micronuclear genome. Protist. 2011;162:616-36 pubmed publisher
    Aphidicolin (APH), an inhibitor of DNA polymerase ?, arrested cell divisions in Tetrahymena thermophila. Surprisingly, low concentrations of APH induced an increase of macronuclear DNA content and cell size in non-dividing cells...
  68. Samaranayake H, Cowan A, Klobutcher L. Vacuolar protein sorting protein 13A, TtVPS13A, localizes to the tetrahymena thermophila phagosome membrane and is required for efficient phagocytosis. Eukaryot Cell. 2011;10:1207-18 pubmed publisher
    ..The TtVPS13A protein was previously identified in a mass spectrometry analysis of the Tetrahymena thermophila phagosome proteome (M. E. Jacobs et al., Eukaryot...
  69. Chang Y, Feng L, Xiong J, Miao W. [Function comparison and evolution analysis of metallothionein gene MTT2 and MTT4 in Tetrahymena thermophila]. Dongwuxue Yanjiu. 2011;32:476-84 pubmed publisher
    ..We used real-time quantitative PCR to test the expression levels of Tetrahymena thermophila metallothionein genes MTT2 and MTT4 after exposure to different inducers, including Hg, Cu, Cd, Zn and H(..
  70. Pucciarelli S, Ballarini P, Sparvoli D, Barchetta S, Yu T, Detrich H, et al. Distinct functional roles of ?-tubulin isotypes in microtubule arrays of Tetrahymena thermophila, a model single-celled organism. PLoS ONE. 2012;7:e39694 pubmed publisher
    ..the functions of the recently discovered, noncanonical ?-like tubulins (BLTs) of the ciliate, Tetrahymena thermophila. Tetrahymena forms 17 distinct microtubular structures whose assembly had been thought to be based on ..
  71. Akiyama B, Gomez A, Stone M. A conserved motif in Tetrahymena thermophila telomerase reverse transcriptase is proximal to the RNA template and is essential for boundary definition. J Biol Chem. 2013;288:22141-9 pubmed publisher
    ..Taken together, these results suggest a model in which the CP2 motif binds the TBE to strictly define which TER nucleotides can be reverse transcribed...
  72. Christensen S, Guerra C, Awan A, Wheatley D, Satir P. Insulin receptor-like proteins in Tetrahymena thermophila ciliary membranes. Curr Biol. 2003;13:R50-2 pubmed
  73. Huvos P. A member of a repeat family is the source of an insertion-deletion polymorphism inside a developmentally eliminated sequence of Tetrahymena thermophila. J Mol Biol. 2004;336:1061-73 pubmed
    In Tetrahymena thermophila, the development of a transcriptionally active macronucleus from a transcriptionally inert micronucleus is accompanied by the elimination of numerous DNA segments, called internally eliminated sequences (IESs), ..
  74. Medzihradszky K, Zhang X, Chalkley R, Guan S, McFarland M, Chalmers M, et al. Characterization of Tetrahymena histone H2B variants and posttranslational populations by electron capture dissociation (ECD) Fourier transform ion cyclotron mass spectrometry (FT-ICR MS). Mol Cell Proteomics. 2004;3:872-86 pubmed publisher
    ..This study establishes that integration of the information from these two datasets provides a comprehensive map of posttranslational occupancy for each particular covalent assemblage selected for structural investigation...
  75. Wiley E, Myers T, Parker K, Braun T, Yao M. Class I histone deacetylase Thd1p affects nuclear integrity in Tetrahymena thermophila. Eukaryot Cell. 2005;4:981-90 pubmed
    ..We previously identified a macronuclear-specific class I HDAC in Tetrahymena thermophila called Thd1p, which is expressed early in the development of the macronucleus when it initially becomes ..
  76. Libusova L, Sulimenko T, Sulimenko V, Janisch R, Hozak P, Draber P. Distinct localization of a beta-tubulin epitope in the Tetrahymena thermophila and Paramecium caudatum cortex. Protoplasma. 2005;225:157-67 pubmed
    ..In Tetrahymena thermophila and Paramecium caudatum, immunofluorescence microscopy with the monoclonal antibody TU-06, directed ..
  77. Cervantes M, Coyne R, Xi X, Yao M. The condensin complex is essential for amitotic segregation of bulk chromosomes, but not nucleoli, in the ciliate Tetrahymena thermophila. Mol Cell Biol. 2006;26:4690-700 pubmed
    The macronucleus of the binucleate ciliate Tetrahymena thermophila contains fragmented and amplified chromosomes that do not have centromeres, eliminating the possibility of mitotic nuclear division...
  78. Jacobs M, DeSouza L, Samaranayake H, Pearlman R, Siu K, Klobutcher L. The Tetrahymena thermophila phagosome proteome. Eukaryot Cell. 2006;5:1990-2000 pubmed
    ..In contrast, unicellular eukaryotes, such as the ciliate Tetrahymena thermophila, employ phagocytosis to ingest and degrade other microorganisms to meet their nutritional needs...
  79. Barchetta S, La Terza A, Ballarini P, Pucciarelli S, Miceli C. Combination of two regulatory elements in the Tetrahymena thermophila HSP70-1 gene controls heat shock activation. Eukaryot Cell. 2008;7:379-86 pubmed
    ..In this study, we investigated the 5' untranslated regions of the Tetrahymena thermophila HSP70-1 gene, and we found, in addition to the canonical and divergent HSEs, multiple sets of GATA ..
  80. Sattin B, Zhao W, Travers K, Chu S, Herschlag D. Direct measurement of tertiary contact cooperativity in RNA folding. J Am Chem Soc. 2008;130:6085-7 pubmed publisher
    ..We precisely measured the stability of an independently folding P4-P6 domain from the Tetrahymena thermophila group I intron by single molecule fluorescence resonance energy transfer (smFRET)...
  81. Balabaskaran Nina P, Dudkina N, Kane L, Van Eyk J, Boekema E, Mather M, et al. Highly divergent mitochondrial ATP synthase complexes in Tetrahymena thermophila. PLoS Biol. 2010;8:e1000418 pubmed publisher
    ..nature of ATP synthase in Apicomplexa, genes for these essential components were absent even in Tetrahymena thermophila, a free-living ciliate belonging to a sister clade of Apicomplexa, which demonstrates robust oxidative ..
  82. Wang Q, Xu J, Chai B, Liang A, Wang W. Functional comparison of metallothioneins MTT1 and MTT2 from Tetrahymena thermophila. Arch Biochem Biophys. 2011;509:170-6 pubmed publisher
    Metallothioneins MTT1 and MTT2 from Tetrahymena thermophila have been characterized. The MTT1 contains mainly characteristic Cys-Cys-Cys and Cys-Cys clusters, but MTT2 contains mainly Cys-X-Cys cluster...
  83. Friman V, Laakso J. Pulsed-resource dynamics constrain the evolution of predator-prey interactions. Am Nat. 2011;177:334-45 pubmed publisher
    ..and ecological dynamics between the prey bacterium Serratia marcescens and the predatory protozoan Tetrahymena thermophila. Predation increased the frequency of defensive, nonpigmented prey types, which bore competitive costs in ..
  84. Andersen K, Nielsen H. Experimental identification and analysis of macronuclear non-coding RNAs from the ciliate Tetrahymena thermophila. Nucleic Acids Res. 2012;40:1267-81 pubmed publisher
    The ciliate Tetrahymena thermophila is an important eukaryotic model organism that has been used in pioneering studies of general phenomena, such as ribozymes, telomeres, chromatin structure and genome reorganization...
  85. Ueno H, Gonda K, Takeda T, Numata O. Identification of elongation factor-1alpha as a Ca2+/calmodulin-binding protein in Tetrahymena cilia. Cell Motil Cytoskeleton. 2003;55:51-60 pubmed
    ..Moreover, EF-1alpha was also localized in cilia of Paramecium, suggesting that EF-1alpha functions as a target protein of Ca(2+)/CaM in ciliate cilia...
  86. Loidl J, Scherthan H. Organization and pairing of meiotic chromosomes in the ciliate Tetrahymena thermophila. J Cell Sci. 2004;117:5791-801 pubmed
    During meiotic prophase in the ciliate Tetrahymena thermophila micronuclei dramatically elongate and form thread-like crescents...
  87. Shiurba R, Hirabayashi T, Masuda M, Kawamura A, Komoike Y, Klitz W, et al. Cellular responses of the ciliate, Tetrahymena thermophila, to far infrared irradiation. Photochem Photobiol Sci. 2006;5:799-807 pubmed
    ..To learn whether they affect cell structure and function, we tested the ciliated protozoan, Tetrahymena thermophila. These unicellular eukaryotes aggregate in swarms near the surface of freshwater habitats, where direct ..
  88. Gallego A, Martín González A, Ortega R, Gutierrez J. Flow cytometry assessment of cytotoxicity and reactive oxygen species generation by single and binary mixtures of cadmium, zinc and copper on populations of the ciliated protozoan Tetrahymena thermophila. Chemosphere. 2007;68:647-61 pubmed
    ..cytometry, the cytotoxicity of the heavy metals Cd, Zn and Cu on populations of the ciliated protozoan Tetrahymena thermophila. The obtained LC(50) for these metals was estimated as 0.195mgCdl(-1), 3.58mgZnl(-1) and 0...
  89. Romi E, Baran N, Gantman M, Shmoish M, Min B, Collins K, et al. High-resolution physical and functional mapping of the template adjacent DNA binding site in catalytically active telomerase. Proc Natl Acad Sci U S A. 2007;104:8791-6 pubmed
    ..photocrosslinking to map interactions between DNA primers and the catalytic protein subunit (tTERT) of Tetrahymena thermophila telomerase in functional enzyme complexes...
  90. Wloga D, Rogowski K, Sharma N, Van Dijk J, Janke C, Edde B, et al. Glutamylation on alpha-tubulin is not essential but affects the assembly and functions of a subset of microtubules in Tetrahymena thermophila. Eukaryot Cell. 2008;7:1362-72 pubmed publisher
    ..Furthermore, the spatial restriction of modifying enzymes appears to be a major mechanism that drives differential glutamylation at the subcellular level...
  91. Zweifel E, Smith J, Romero D, Giddings T, Winey M, Honts J, et al. Nested genes CDA12 and CDA13 encode proteins associated with membrane trafficking in the ciliate Tetrahymena thermophila. Eukaryot Cell. 2009;8:899-912 pubmed publisher
    We describe a novel pair of nested genes, CDA12 and CDA13, from Tetrahymena thermophila. Both are implicated in membrane trafficking associated with cell division and conjugation...
  92. Nusblat A, Najle S, Tomazic M, Uttaro A, Nudel C. C-5(6) sterol desaturase from tetrahymena thermophila: Gene identification and knockout, sequence analysis, and comparison to other C-5(6) sterol desaturases. Eukaryot Cell. 2009;8:1287-97 pubmed publisher
    The gene coding for a C-5(6) sterol desaturase in Tetrahymena thermophila, DES5A, has been identified by the knockout of the TTHERM_01194720 sequence...