cryptosporidium parvum

Summary

Summary: A species of parasitic protozoa that infects humans and most domestic mammals. Its oocysts measure five microns in diameter. These organisms exhibit alternating cycles of sexual and asexual reproduction.

Top Publications

  1. Xiao L. Molecular epidemiology of cryptosporidiosis: an update. Exp Parasitol. 2010;124:80-9 pubmed publisher
    ..The wide use of a new generation of genotyping and subtyping tools in well designed epidemiologic studies should lead to a more in-depth understanding of the epidemiology of cryptosporidiosis in humans and animals. ..
  2. Robinson G, Chalmers R. Assessment of polymorphic genetic markers for multi-locus typing of Cryptosporidium parvum and Cryptosporidium hominis. Exp Parasitol. 2012;132:200-15 pubmed publisher
    The use of high resolution molecular tools to study Cryptosporidium parvum and Cryptosporidium hominis intra-species variation is becoming common practice, but there is currently no consensus in the methods used...
  3. O Connor R, Burns P, Ha Ngoc T, Scarpato K, Khan W, Kang G, et al. Polymorphic mucin antigens CpMuc4 and CpMuc5 are integral to Cryptosporidium parvum infection in vitro. Eukaryot Cell. 2009;8:461-9 pubmed publisher
    ..and CpMuc5 were selected for further investigation because of the significant sequence divergence between Cryptosporidium parvum and C. hominis alleles. Rabbit anti-CpMuc5 and -CpMuc4 antibodies identified several polypeptides in C...
  4. Quilez J, Vergara Castiblanco C, Monteagudo L, del Cacho E, Sanchez Acedo C. Host association of Cryptosporidium parvum populations infecting domestic ruminants in Spain. Appl Environ Microbiol. 2013;79:5363-71 pubmed publisher
    A stock of 148 Cryptosporidium parvum DNA extracts from lambs and goat kids selected from a previous study examining the occurrence of Cryptosporidium species and GP60 subtypes in diarrheic lambs and goat kids in northeastern Spain was ..
  5. Hoek M, Oliver I, Barlow M, Heard L, Chalmers R, Paynter S. Outbreak of Cryptosporidium parvum among children after a school excursion to an adventure farm, south west England. J Water Health. 2008;6:333-8 pubmed
    We present the results of an outbreak investigation on a Cryptosporidium parvum outbreak among 35 people, (27 students and 8 teachers), who participated in a school excursion to an outdoor adventure farm in South West England, from 22 to ..
  6. Mauzy M, Enomoto S, Lancto C, Abrahamsen M, Rutherford M. The Cryptosporidium parvum transcriptome during in vitro development. PLoS ONE. 2012;7:e31715 pubmed publisher
    ..The transcript abundance for 3,302 genes (87%) of the Cryptosporidium parvum protein coding genome was elucidated over a 72 hr infection within HCT8 cells using Real Time-PCR...
  7. Takeuchi D, Jones V, Kobayashi M, Suzuki F. Cooperative role of macrophages and neutrophils in host Antiprotozoan resistance in mice acutely infected with Cryptosporidium parvum. Infect Immun. 2008;76:3657-63 pubmed publisher
    Severe experimental infections with Cryptosporidium parvum have been reported in immunocompromised animals such as SCID mice (mice without functional T cells and B cells). In a C...
  8. Perez Cordon G, Nie W, Schmidt D, Tzipori S, Feng H. Involvement of host calpain in the invasion of Cryptosporidium parvum. Microbes Infect. 2011;13:103-7 pubmed publisher
    b>Cryptosporidium parvum induces the formation of an actin-dense plaque which is essential for the successful invasion of epithelial cells. Host molecules that are involved in the regulation of this cytoskeleton reorganization are unknown...
  9. Soba B, Logar J. Genetic classification of Cryptosporidium isolates from humans and calves in Slovenia. Parasitology. 2008;135:1263-70 pubmed publisher
    ..In humans, C. hominis Ia (subtype IaA17R3) and Ib (IbA10G2) and Cryptosporidium parvum IIa (IIaA9G1R1, IIaA11G2R1, IIaA13R1, IIaA14G1R1, IIaA15G1R1, IIaA15G2R1, IIaA16G1R1, IIaA17G1R1 and ..

More Information

Publications92

  1. Amer S, Matsubara R, Murakoshi F, Nakai Y. Molecular analysis of Cryptosporidium parvum HNJ-1 isolated in Japan. J Vet Med Sci. 2010;72:1647-9 pubmed
    b>Cryptosporidium parvum HNJ-1 is widely used as a reference strain in Japan...
  2. Schnyder M, Kohler L, Hemphill A, Deplazes P. Prophylactic and therapeutic efficacy of nitazoxanide against Cryptosporidium parvum in experimentally challenged neonatal calves. Vet Parasitol. 2009;160:149-54 pubmed publisher
    Diarrhoea caused by Cryptosporidium parvum is a major problem in calves younger than 4 weeks of age. To date only a few compounds have been approved for prophylactic and none for therapeutic use...
  3. Jex A, Gasser R. Analysis of the genetic diversity within Cryptosporidium hominis and Cryptosporidium parvum from imported and autochtonous cases of human cryptosporidiosis by mutation scanning. Electrophoresis. 2008;29:4119-29 pubmed publisher
    ..variation in part of the 60 kilodalton glycoprotein (pgp60) gene among Cryptosporidium hominis and Cryptosporidium parvum isolates (n=115) from citizens of the UK inferred to have been infected whilst travelling abroad (to 25 ..
  4. Boulter Bitzer J, Lee H, Trevors J. Single chain variable fragment antibodies selected by phage display against the sporozoite surface antigen S16 of Cryptosporidium parvum. Exp Parasitol. 2010;125:124-9 pubmed publisher
    ..fragment (scFv) libraries were used to select clones that bound to the surface glycoprotein S16 of Cryptosporidium parvum. Panning of the Tomlinson libraries I and J resulted in the isolation of nine distinct clones...
  5. Bessoff K, Sateriale A, Lee K, Huston C. Drug repurposing screen reveals FDA-approved inhibitors of human HMG-CoA reductase and isoprenoid synthesis that block Cryptosporidium parvum growth. Antimicrob Agents Chemother. 2013;57:1804-14 pubmed publisher
    Cryptosporidiosis, a diarrheal disease usually caused by Cryptosporidium parvum or Cryptosporidium hominis in humans, can result in fulminant diarrhea and death in AIDS patients and chronic infection and stunting in children...
  6. Macarisin D, Bauchan G, Fayer R. Spinacia oleracea L. leaf stomata harboring Cryptosporidium parvum oocysts: a potential threat to food safety. Appl Environ Microbiol. 2010;76:555-9 pubmed publisher
    b>Cryptosporidium parvum is a cosmopolitan microscopic protozoan parasite that causes severe diarrheal disease (cryptosporidiosis) in mammals, including humans and livestock...
  7. Certad G, Benamrouz S, Guyot K, Mouray A, Chassat T, Flament N, et al. Fulminant cryptosporidiosis after near-drowning: a human Cryptosporidium parvum strain implicated in invasive gastrointestinal adenocarcinoma and cholangiocarcinoma in an experimental model. Appl Environ Microbiol. 2012;78:1746-51 pubmed publisher
    In the present work, we report the characterization of a Cryptosporidium parvum strain isolated from a patient who nearly drowned in the Deule River (Lille, France) after being discharged from the hospital where he had undergone ..
  8. Zhang H, Guo F, Zhu G. Involvement of host cell integrin ?2 in Cryptosporidium parvum infection. Infect Immun. 2012;80:1753-8 pubmed publisher
    b>Cryptosporidium parvum is an opportunistic pathogen in AIDS patients. It is an intracellular but extracytoplasmic parasite residing in a host cell-derived parasitophorous vacuole...
  9. Adell A, Miller W, Harvey D, Vanwormer E, Wuertz S, Conrad P. Individual subject meta-analysis of parameters for Cryptosporidium parvum shedding and diarrhoea in animal experimental models. Epidemiol Infect. 2013;141:1662-78 pubmed publisher
    ..Additionally, detection of possible publication bias highlights the need to publish additional studies that convey statistically non-significant as well as significant results in the future...
  10. Zhang H, Guo F, Zhou H, Zhu G. Transcriptome analysis reveals unique metabolic features in the Cryptosporidium parvum Oocysts associated with environmental survival and stresses. BMC Genomics. 2012;13:647 pubmed publisher
    b>Cryptosporidium parvum is a globally distributed zoonotic parasite and an important opportunistic pathogen in immunocompromised patients...
  11. Das G, Changkija B, Sarkar S, Das P. Genotyping of Cryptosporidium parvum isolates in bovine population in Kolkata and characterization of new bovine genotypes. Res Vet Sci. 2011;91:246-50 pubmed publisher
    ..Further study will be required to understand the phylogenetic origin and functional significance in virulence and morbidity of this genotype. ..
  12. Yamagishi J, Wakaguri H, Sugano S, Kawano S, Fujisaki K, Sugimoto C, et al. Construction and analysis of full-length cDNA library of Cryptosporidium parvum. Parasitol Int. 2011;60:199-202 pubmed publisher
    A full-length cDNA library was constructed from the sporozoite of Cryptosporidium parvum. Normalized clones were subjected to Solexa shotgun sequencing, and then complete sequences for 1066 clones were reconfigured...
  13. Jenkins M, O Brien C, Miska K, Schwarz R, Karns J, Santin M, et al. Gene expression during excystation of Cryptosporidium parvum oocysts. Parasitol Res. 2011;109:509-13 pubmed publisher
    ..describes transcription of mRNA from genes encoding metabolic or structural proteins during excystation of Cryptosporidium parvum oocysts. RNA was harvested from C...
  14. Sheoran A, Wiffin A, Widmer G, Singh P, Tzipori S. Infection with Cryptosporidium hominis provides incomplete protection of the host against Cryptosporidium parvum. J Infect Dis. 2012;205:1019-23 pubmed publisher
    Cryptosporidium hominis and Cryptosporidium parvum, which infect humans equally, are genetically/antigenically almost identical. It remains unclear, however, whether infection with C. hominis protects against C. parvum...
  15. Castro I, Oliveira B, Slowikowski J, Coutinho B, Siqueira F, Costa L, et al. Arginine decreases Cryptosporidium parvum infection in undernourished suckling mice involving nitric oxide synthase and arginase. Nutrition. 2012;28:678-85 pubmed publisher
    This study investigated the role of L-arginine supplementation to undernourished and Cryptosporidium parvum-infected suckling mice. The following regimens were initiated on the fourth day of life and injected subcutaneously daily. The C...
  16. McNair N, Mead J. CD4? effector and memory cell populations protect against Cryptosporidium parvum infection. Microbes Infect. 2013;15:599-606 pubmed publisher
    b>Cryptosporidium parvum is a protozoan parasite that infects the epithelial cells of the small intestine causing diarrheal illness in humans...
  17. Waldron L, Ferrari B, Power M. Glycoprotein 60 diversity in C. hominis and C. parvum causing human cryptosporidiosis in NSW, Australia. Exp Parasitol. 2009;122:124-7 pubmed publisher
    ..The subtypes identified have global distributions and indicate that anthroponotic and zoonotic transmission routes contribute to sporadic human cryptosporidiosis in NSW. ..
  18. Kar S, Gawlowska S, Daugschies A, Bangoura B. Quantitative comparison of different purification and detection methods for Cryptosporidium parvum oocysts. Vet Parasitol. 2011;177:366-70 pubmed publisher
    b>Cryptosporidium parvum (C. parvum) is the causal agent of cryptosporidiosis in many animals, mainly cattle, and possesses a high zoonotic potential. It occurs worldwide and ubiquitously. Detection of C...
  19. Guo A, Yin J, Xiang M, Liu X, Zhang Y, Chen Q. [Screening for relevant proteins involved in adhesion of Cryptosporidium parvum sporozoites to host cells]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2009;27:87-8 pubmed
    The Cryptosporidium parvum T7 phage display library was screened by using Caco-2 cells...
  20. Wolyniak E, Hargreaves B, Jellison K. Retention and release of Cryptosporidium parvum oocysts by experimental biofilms composed of a natural stream microbial community. Appl Environ Microbiol. 2009;75:4624-6 pubmed publisher
    b>Cryptosporidium parvum oocysts accumulate on biofilm surfaces...
  21. Ctrnacta V, Fritzler J, Surinová M, Hrdy I, Zhu G, Stejskal F. Efficacy of S-adenosylhomocysteine hydrolase inhibitors, D-eritadenine and (S)-DHPA, against the growth of Cryptosporidium parvum in vitro. Exp Parasitol. 2010;126:113-6 pubmed publisher
    ..that these two compounds also display efficacy against recombinant SAHH enzyme of the protozoan parasite Cryptosporidium parvum, as well as inhibition of parasite growth in vitro...
  22. Szonyi B, Bordonaro R, Wade S, Mohammed H. Seasonal variation in the prevalence and molecular epidemiology of Cryptosporidium infection in dairy cattle in the New York City Watershed. Parasitol Res. 2010;107:317-25 pubmed publisher
    ..The overall prevalence of Cryptosporidium parvum-like species and Cryptosporidium andersoni among the 1911 animals tested by the flotation method was 5% ..
  23. Garvey M, Farrell H, Cormican M, Rowan N. Investigations of the relationship between use of in vitro cell culture-quantitative PCR and a mouse-based bioassay for evaluating critical factors affecting the disinfection performance of pulsed UV light for treating Cryptosporidium parvum oocysts . J Microbiol Methods. 2010;80:267-73 pubmed publisher
    b>Cryptosporidium parvum is an enteric coccidian parasite that is recognised as a frequent cause of water-borne disease in humans...
  24. Jenkins M, Eaglesham B, Anthony L, Kachlany S, Bowman D, Ghiorse W. Significance of wall structure, macromolecular composition, and surface polymers to the survival and transport of Cryptosporidium parvum oocysts. Appl Environ Microbiol. 2010;76:1926-34 pubmed publisher
    ..composition of the oocyst wall are primary factors determining the survival and hydrologic transport of Cryptosporidium parvum oocysts outside the host...
  25. Kang J, Ju H, Yu J, Sohn W, Na B. Cryptostatin, a chagasin-family cysteine protease inhibitor of Cryptosporidium parvum. Parasitology. 2012;139:1029-37 pubmed publisher
    ..this study, we identified and characterized cryptostatin, a novel inhibitor of cysteine protease (ICP) of Cryptosporidium parvum. Cryptostatin showed low sequence identity to other chagasin-family ICPs, but 3 motifs (NPTTG, GXGG, and ..
  26. Artz J, Dunford J, Arrowood M, Dong A, Chruszcz M, Kavanagh K, et al. Targeting a uniquely nonspecific prenyl synthase with bisphosphonates to combat cryptosporidiosis. Chem Biol. 2008;15:1296-306 pubmed publisher
    ..We present a study demonstrating nitrogen-containing bisphosphonates (N-BPs) to be capable of inhibiting Cryptosporidium parvum at low micromolar concentrations in infected MDCK cells...
  27. Elsafi S, Al Maqati T, Hussein M, Adam A, Hassan M, Al Zahrani E. Comparison of microscopy, rapid immunoassay, and molecular techniques for the detection of Giardia lamblia and Cryptosporidium parvum. Parasitol Res. 2013;112:1641-6 pubmed publisher
    Giardia lamblia and Cryptosporidium parvum are recognized as the most common protozoan infections in Saudi Arabia. Microscopic examination of stool samples, either direct or concentrated, for the recovery of G...
  28. Cama V, Bern C, Roberts J, Cabrera L, Sterling C, Ortega Y, et al. Cryptosporidium species and subtypes and clinical manifestations in children, Peru. Emerg Infect Dis. 2008;14:1567-74 pubmed publisher
    ..genotypes or subtypes. These findings strongly suggest that Cryptosporidium spp. and subtypes are linked to different clinical manifestations in children...
  29. Quilez J, Vergara Castiblanco C, Monteagudo L, del Cacho E, Sanchez Acedo C. Multilocus fragment typing and genetic structure of Cryptosporidium parvum Isolates from diarrheic preweaned calves in Spain. Appl Environ Microbiol. 2011;77:7779-86 pubmed publisher
    A collection of 140 Cryptosporidium parvum isolates previously analyzed by PCR-restriction fragment length polymorphism (PCR-RFLP) and sequence analyses of the small-subunit (SSU) rRNA and 60-kDa glycoprotein (GP60) genes was further ..
  30. Certad G, Creusy C, Guyot K, Mouray A, Chassat T, Delaire B, et al. Fulminant cryptosporidiosis associated with digestive adenocarcinoma in SCID mice infected with Cryptosporidium parvum TUM1 strain. Int J Parasitol. 2010;40:1469-75 pubmed publisher
    We recently demonstrated that Cryptosporidium parvum IOWA strain induces in situ ileo-caecal adenocarcinoma in an animal model. Herein, the ability of another C...
  31. O Hara S, Bogert P, Trussoni C, Chen X, Larusso N. TLR4 promotes Cryptosporidium parvum clearance in a mouse model of biliary cryptosporidiosis. J Parasitol. 2011;97:813-21 pubmed publisher
    ..by gram-negative lipopolysaccharide (LPS), is upregulated in cholangiocytes in response to infection with Cryptosporidium parvum in vitro and contributes to nuclear factor-kappaB (NF-kB) activation...
  32. Wernimont A, Artz J, Finerty P, Lin Y, Amani M, Allali Hassani A, et al. Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium. Nat Struct Mol Biol. 2010;17:596-601 pubmed publisher
    ..and activated (calcium-bound) conformations of CDPKs from the apicomplexan parasites Toxoplasma gondii and Cryptosporidium parvum. In the apo form, the C-terminal CDPK activation domain (CAD) resembles a calmodulin protein with an ..
  33. Shields J, Lee M, Murphy H. Use of a common laboratory glassware detergent improves recovery of Cryptosporidium parvum and Cyclospora cayetanensis from lettuce, herbs and raspberries. Int J Food Microbiol. 2012;153:123-8 pubmed publisher
    ..b>Cryptosporidium parvum and Cyclospora cayetanensis oocysts were seeded onto herbs, lettuces and raspberries, eluted with one of ..
  34. Joung M, Yoon S, Choi K, Kim J, Park W, Yu J. Characterization of the thioredoxin peroxidase from Cryptosporidium parvum. Exp Parasitol. 2011;129:331-6 pubmed publisher
    b>Cryptosporidium parvum can survive exposure to harsh environmental conditions, various disinfectants, and high doses of ?-radiation. Recently, it was found that the expression of thioredoxin peroxidase (CpTPx) in C...
  35. Certad G, Creusy C, Ngouanesavanh T, Guyot K, Gantois N, Mouray A, et al. Development of Cryptosporidium parvum-induced gastrointestinal neoplasia in severe combined immunodeficiency (SCID) mice: severity of lesions is correlated with infection intensity. Am J Trop Med Hyg. 2010;82:257-65 pubmed publisher
    We reported previously that Cryptosporidium parvum was able to induce intestinal tumors in severe combined immunodeficiency (SCID) mice treated with corticoids...
  36. Riera T, Wang W, Josephine H, Hedstrom L. A kinetic alignment of orthologous inosine-5'-monophosphate dehydrogenases. Biochemistry. 2008;47:8689-96 pubmed publisher
    ..catalytic cycle, we have delineated the kinetic mechanism of IMPDH from the pathogenic protozoan parasite Cryptosporidium parvum ( CpIMPDH), which was obtained from a bacterial source through horizontal gene transfer, and its host ..
  37. Zhang L, Sheoran A, Widmer G. Cryptosporidium parvum DNA replication in cell-free culture. J Parasitol. 2009;95:1239-42 pubmed publisher
    ..For reasons that are not clear, development rapidly declines within 2-3 days. The unexpected report of Cryptosporidium parvum culture in the absence of host cells, and the failure of others to reproduce the method, prompted us to ..
  38. Imre K, Luca C, Costache M, Sala C, Morar A, Morariu S, et al. Zoonotic Cryptosporidium parvum in Romanian newborn lambs (Ovis aries). Vet Parasitol. 2013;191:119-22 pubmed publisher
    ..The identified species were: Cryptosporidium parvum (20/24), C. ubiquitum (2/24) and C. xiaoi (2/24), respectively. PCR-RFLP results for C. ubiquitum and C...
  39. Lee J, Han E, Huh S, Park W, Yu J. A hospital-based serological survey of cryptosporidiosis in the Republic of Korea. Korean J Parasitol. 2009;47:219-25 pubmed publisher
    ..ELISA was used to measure antibody titers against Cryptosporidium parvum antigens from a total of 2,394 serum samples, which were collected randomly from patients in local ..
  40. Zintl A, Keogh B, Ezzaty Mirhashemi M, de Waal T, Scholz D, Mulcahy G. Survival of Cryptosporidium parvum oocysts in the presence of hydrated lime. Vet Rec. 2010;166:297-300 pubmed publisher
    ..Although the addition of 5 or 10 per cent lime caused the total number of oocysts to decrease, there appeared to be an increase in the proportion of potentially viable oocysts...
  41. Wolyniak E, Hargreaves B, Jellison K. Seasonal retention and release of Cryptosporidium parvum oocysts by environmental biofilms in the laboratory. Appl Environ Microbiol. 2010;76:1021-7 pubmed publisher
  42. Al Mathal E, Alsalem A. Pomegranate (Punica granatum) peel is effective in a murine model of experimental Cryptosporidium parvum. Exp Parasitol. 2012;131:350-7 pubmed publisher
    Cryptosporidiosis, a major health issue for neonatal calves, is caused by the parasite Cryptosporidium parvum, which is highly resistant to drug treatments...
  43. Xu T, Ping J, Yu Y, Yu F, Yu Y, Hao P, et al. Revealing parasite influence in metabolic pathways in Apicomplexa infected patients. BMC Bioinformatics. 2010;11 Suppl 11:S13 pubmed publisher
    ..A new analytical method was designed in this work to study host-parasite interactions in human cells infected with Plasmodium falciparum and Cryptosporidium parvum.
  44. Yang Y, Buck G, Widmer G. Cell sorting-assisted microarray profiling of host cell response to Cryptosporidium parvum infection. Infect Immun. 2010;78:1040-8 pubmed publisher
    ..the transcriptional response of mammalian cells to infection with the intracellular apicomplexan parasite Cryptosporidium parvum, infected and uninfected cells were recovered from C. parvum-infected cell monolayers...
  45. Boulter Bitzer J, Lee H, Trevors J. Single-chain variable fragment antibodies selected by phage display against the sporozoite surface antigen P23 Of Cryptosporidium parvum. J Parasitol. 2009;95:75-81 pubmed publisher
    b>Cryptosporidium parvum, an apicomplexan parasite transmitted via animal fecal wastes, is the causative agent of cryptosporidiosis...
  46. DiCesare E, Hargreaves B, Jellison K. Biofilm roughness determines Cryptosporidium parvum retention in environmental biofilms. Appl Environ Microbiol. 2012;78:4187-93 pubmed publisher
    ..Better understanding of factors controlling biofilm retention of oocysts should improve our understanding of oocyst transport at different scales...
  47. O Hara S, Small A, Gajdos G, Badley A, Chen X, Larusso N. HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum. J Infect Dis. 2009;199:1195-204 pubmed publisher
    ..Infection of biliary epithelial cells (cholangiocytes) with Cryptosporidium parvum induces Toll-like receptor (TLR) 4 expression and stimulates a TLR-dependent response against infection...
  48. Cook W, Senkovich O, Chattopadhyay D. An unexpected phosphate binding site in glyceraldehyde 3-phosphate dehydrogenase: crystal structures of apo, holo and ternary complex of Cryptosporidium parvum enzyme. BMC Struct Biol. 2009;9:9 pubmed publisher
  49. Liu Y, Janjaroen D, Kuhlenschmidt M, Kuhlenschmidt T, Nguyen T. Deposition of Cryptosporidium parvum oocysts on natural organic matter surfaces: microscopic evidence for secondary minimum deposition in a radial stagnation point flow cell. Langmuir. 2009;25:1594-605 pubmed publisher
    ..point flow (RSPF) system combined with a microscope was used to determine the deposition kinetics of Cryptosporidium parvum oocysts on quartz surfaces and silica surfaces coated with Suwannee River natural organic matter (SRNOM) ..
  50. Schmidt J, Kuhlenschmidt M. Microbial adhesion of Cryptosporidium parvum: identification of a colostrum-derived inhibitory lipid. Mol Biochem Parasitol. 2008;162:32-9 pubmed publisher
    ..described an unidentified lipid purified from calf small intestine that inhibits the in vitro adhesion of Cryptosporidium parvum sporozoites to host cells [Johnson JK, Schmidt J, Gelberg HB, Kuhlenschmidt MS...
  51. Diaz P, Hadfield S, Quilez J, Soilán M, Lopez C, Panadero R, et al. Assessment of three methods for multilocus fragment typing of Cryptosporidium parvum from domestic ruminants in north west Spain. Vet Parasitol. 2012;186:188-95 pubmed publisher
    ..CE), high resolution slab-gel electrophoresis and sequencing, for PCR fragment size analysis of two Cryptosporidium parvum microsatellite regions, ML1 and ML2, was investigated by analysing 27 isolates from calves and 14 from ..
  52. Feng Y, Torres E, Li N, Wang L, Bowman D, Xiao L. Population genetic characterisation of dominant Cryptosporidium parvum subtype IIaA15G2R1. Int J Parasitol. 2013;43:1141-7 pubmed publisher
    The subtype IIaA15G2R1 at the 60 kDa glycoprotein (gp60) gene locus is the most dominant Cryptosporidium parvum infecting dairy cattle and humans in industrialised nations...
  53. Ehrenman K, Wanyiri J, Bhat N, Ward H, Coppens I. Cryptosporidium parvum scavenges LDL-derived cholesterol and micellar cholesterol internalized into enterocytes. Cell Microbiol. 2013;15:1182-97 pubmed publisher
    ..b>Cryptosporidium parvum has minimal anabolic capabilities and relies on transporters and salvage pathways to meet its basic ..
  54. Codices V, Martins C, Novo C, de Sousa B, Lopes A, Borrego M, et al. Dynamics of cytokines and immunoglobulins serum profiles in primary and secondary Cryptosporidium parvum infection: usefulness of Luminex® xMAP technology. Exp Parasitol. 2013;133:106-13 pubmed publisher
    Infection by Cryptosporidium parvum triggers a complex array of innate and adaptive cell mediated immune response, playing an important role in controlling the infection...
  55. Gorla S, Kavitha M, Zhang M, Liu X, Sharling L, Gollapalli D, et al. Selective and potent urea inhibitors of cryptosporidium parvum inosine 5'-monophosphate dehydrogenase. J Med Chem. 2012;55:7759-71 pubmed publisher
    b>Cryptosporidium parvum and related species are zoonotic intracellular parasites of the intestine. Cryptosporidium is a leading cause of diarrhea in small children around the world...
  56. Costa L, Johnbull E, Reeves J, Sevilleja J, Freire R, Hoffman P, et al. Cryptosporidium-malnutrition interactions: mucosal disruption, cytokines, and TLR signaling in a weaned murine model. J Parasitol. 2011;97:1113-20 pubmed publisher
    ..Depressed TLR2 and 4 signaling and Th1 cytokine response may be important in the mechanisms underlying the vicious cycle of malnutrition and enteric infection...
  57. Pokorny N, Boulter Bitzer J, Hall J, Trevors J, Lee H. Inhibition of Cryptosporidium parvum infection of a mammalian cell culture by recombinant scFv antibodies. Antonie Van Leeuwenhoek. 2008;94:353-64 pubmed publisher
    Two phage display antibody libraries (Tomlinson I and J) were screened against the whole oocysts of Cryptosporidium parvum to select for scFv (single chain variable fragment) antibodies. Three scFv antibodies were selected that bound to C...
  58. Wastling J, Xia D, Sohal A, Chaussepied M, Pain A, Langsley G. Proteomes and transcriptomes of the Apicomplexa--where's the message?. Int J Parasitol. 2009;39:135-43 pubmed publisher
    ..Toxoplasma gondii, Cryptosporidium parvum, Neospora caninum and Theileria spp...
  59. Majewska A, Graczyk T, Słodkowicz Kowalska A, Tamang L, Jedrzejewski S, Zduniak P, et al. The role of free-ranging, captive, and domestic birds of Western Poland in environmental contamination with Cryptosporidium parvum oocysts and Giardia lamblia cysts. Parasitol Res. 2009;104:1093-9 pubmed publisher
    As Cryptosporidium parvum and Giardia lamblia can be disseminated in the environment by avian hosts, a total of 499 fecal dropping from 308 free-ranging, 90 captive, and 101 domestic birds were tested by conventional, immunological, and ..
  60. BAJSZAR G, Dekonenko A. Stress-induced Hsp70 gene expression and inactivation of Cryptosporidium parvum oocysts by chlorine-based oxidants. Appl Environ Microbiol. 2010;76:1732-9 pubmed publisher
    Our research on the mechanisms of action of chlorine-based oxidants on Cryptosporidium parvum oocysts in water revealed a dual-phase effect: (i) response to oxidative stress, which was demonstrated by induced expression of the Hsp70 heat ..
  61. Mohanram A, Ray C, Harvey R, Metge D, Ryan J, Chorover J, et al. Comparison of transport and attachment behaviors of Cryptosporidium parvum oocysts and oocyst-sized microspheres being advected through three minerologically different granular porous media. Water Res. 2010;44:5334-44 pubmed publisher
    In order to gain more information about the fate of Cryptosporidium parvum oocysts in tropical volcanic soils, the transport and attachment behaviors of oocysts and oocyst-sized polystyrene microspheres were studied in the presence of two ..
  62. Yakoob J, Abbas Z, Beg M, Naz S, Khan R, Islam M, et al. Prevalences of Giardia lamblia and Cryptosporidium parvum infection in adults presenting with chronic diarrhoea. Ann Trop Med Parasitol. 2010;104:505-10 pubmed publisher
    Giardia lamblia and Cryptosporidium parvum are both waterborne pathogens associated with diarrhoea in developing countries...
  63. Quah J, Ambu S, Lim Y, Mahdy M, Mak J. Molecular identification of Cryptosporidium parvum from avian hosts. Parasitology. 2011;138:573-7 pubmed publisher
    ..9%) produced amplicons using nested PCR. Phylogenetic trees identified all the isolates as Cryptosporidium parvum. Although C...
  64. Lee S, Joung M, Nam T, Park W, Yu J. Rejoining of gamma-ray-induced DNA damage in Cryptosporidium parvum measured by the comet assay. Exp Parasitol. 2010;125:230-5 pubmed publisher
    b>Cryptosporidium parvum is a well-known waterborne intracellular protozoan that causes severe diarrheal illness in immunocompromised individuals...
  65. Zhou R, Hu G, Liu J, Gong A, Drescher K, Chen X. NF-kappaB p65-dependent transactivation of miRNA genes following Cryptosporidium parvum infection stimulates epithelial cell immune responses. PLoS Pathog. 2009;5:e1000681 pubmed publisher
    b>Cryptosporidium parvum is a protozoan parasite that infects the gastrointestinal epithelium and causes diarrheal disease worldwide. Innate epithelial immune responses are key mediators of the host's defense to C. parvum...
  66. O Hara S, Splinter P, Gajdos G, Trussoni C, Fernandez Zapico M, Chen X, et al. NFkappaB p50-CCAAT/enhancer-binding protein beta (C/EBPbeta)-mediated transcriptional repression of microRNA let-7i following microbial infection. J Biol Chem. 2010;285:216-25 pubmed publisher
    ..Here, we identify a novel transcriptional mechanism induced by the protozoan parasite Cryptosporidium parvum and Gram(-) bacteria-derived lipopolysaccharide (LPS) mediating let-7i promoter silencing in human biliary ..
  67. Brockmann S, Dreweck C, Wagner Wiening C, Hagen R, Kimmig P, Petry F, et al. Serological and epidemiological analysis of an outbreak of gastroenteritis among military recruits in Germany caused by Cryptosporidium parvum. Infection. 2008;36:450-7 pubmed publisher
    ..Cryptosporidium spp. cause enteritic disease worldwide. Besides those patients with an impaired immune system, the general population is also at risk...
  68. Korbel D, Barakat F, Di Santo J, McDonald V. CD4+ T cells are not essential for control of early acute Cryptosporidium parvum infection in neonatal mice. Infect Immun. 2011;79:1647-53 pubmed publisher
    ..that infect epithelial cells. Recovery from Cryptosporidium parvum infection in adult hosts involves CD4(+) T cells with a strong Th1 component, but mechanisms of immunity ..
  69. Caccio S, Sannella A, Mariano V, Valentini S, Berti F, Tosini F, et al. A rare Cryptosporidium parvum genotype associated with infection of lambs and zoonotic transmission in Italy. Vet Parasitol. 2013;191:128-31 pubmed publisher
    ..DNA was extracted from an archived slide and from fecal specimens, and the parasite was identified as Cryptosporidium parvum by PCR and sequence analysis at the CpA135 gene...
  70. Borowski H, Thompson R, Armstrong T, Clode P. Morphological characterization of Cryptosporidium parvum life-cycle stages in an in vitro model system. Parasitology. 2010;137:13-26 pubmed publisher
    b>Cryptosporidium parvum is a zoonotic protozoan parasite that mainly affects the ileum of humans and livestock, with the potential to cause severe enteric disease. We describe the complete life cycle of C. parvum in an in vitro system...
  71. Gong A, Hu G, Zhou R, Liu J, Feng Y, Soukup G, et al. MicroRNA-221 controls expression of intercellular adhesion molecule-1 in epithelial cells in response to Cryptosporidium parvum infection. Int J Parasitol. 2011;41:397-403 pubmed publisher
    b>Cryptosporidium parvum is a protozoan parasite that infects gastrointestinal epithelial cells and causes diarrhoeal disease in humans and animals globally. Pathological changes following C...
  72. Shahiduzzaman M, Dyachenko V, Khalafalla R, Desouky A, Daugschies A. Effects of curcumin on Cryptosporidium parvum in vitro. Parasitol Res. 2009;105:1155-61 pubmed publisher
    b>Cryptosporidium parvum is a zoonotic protozoan parasite having peculiarities among the apicomplexa that could be responsible for its resistance to some drugs and disinfectants against coccidia...
  73. Rider S, Zhu G. Cryptosporidium: genomic and biochemical features. Exp Parasitol. 2010;124:2-9 pubmed publisher
    Recent progress in understanding the unique biochemistry of the two closely related human enteric pathogens Cryptosporidium parvum and Cryptosporidium hominis has been stimulated by the elucidation of the complete genome sequences for ..
  74. Kirubakaran S, Gorla S, Sharling L, Zhang M, Liu X, Ray S, et al. Structure-activity relationship study of selective benzimidazole-based inhibitors of Cryptosporidium parvum IMPDH. Bioorg Med Chem Lett. 2012;22:1985-8 pubmed publisher
    Cryptosporidium parasites are important waterborne pathogens of both humans and animals. The Cryptosporidium parvum and Cryptosporidium hominis genomes indicate that the only route to guanine nucleotides is via inosine 5'-monophosphate ..
  75. De Waele V, Van den Broeck F, Huyse T, McGrath G, Higgins I, Speybroeck N, et al. Panmictic structure of the Cryptosporidium parvum population in Irish calves: influence of prevalence and host movement. Appl Environ Microbiol. 2013;79:2534-41 pubmed publisher
    In total, 245 Cryptosporidium parvum specimens obtained from calves in 205 Irish herds between 2003 and 2005 were subtyped by sequencing the glycoprotein gene gp60 and performing multilocus analysis of seven markers...
  76. King B, Hoefel D, Lim S, Robinson B, Monis P. Flow cytometric assessment of distinct physiological stages within Cryptosporidium parvum sporozoites post-excystation. Parasitology. 2009;136:953-66 pubmed publisher
    b>Cryptosporidium parvum are protozoan parasites responsible for outbreaks of gastrointestinal disease worldwide...
  77. Abudalo R, Ryan J, Harvey R, Metge D, Landkamer L. Influence of organic matter on the transport of Cryptosporidium parvum oocysts in a ferric oxyhydroxide-coated quartz sand saturated porous medium. Water Res. 2010;44:1104-13 pubmed publisher
    To assess the effect of organic matter on the transport of Cryptosporidium parvum oocysts in a geochemically heterogeneous saturated porous medium, we measured the breakthrough and collision efficiencies of oocysts as a function of ..
  78. Zhang Z, Ojo K, Johnson S, Larson E, He P, Geiger J, et al. Benzoylbenzimidazole-based selective inhibitors targeting Cryptosporidium parvum and Toxoplasma gondii calcium-dependent protein kinase-1. Bioorg Med Chem Lett. 2012;22:5264-7 pubmed publisher
    Calcium-dependent protein kinase-1 (CDPK1) from Cryptosporidium parvum (CpCDPK1) and Toxoplasma gondii (TgCDPK1) have become attractive targets for discovering selective inhibitors to combat infections caused by these protozoa...
  79. Lee S, Joung M, Nam T, Park W, Yu J. Quantitative evaluation of infectivity change of Cryptosporidium parvum after gamma irradiation. Korean J Parasitol. 2009;47:7-11 pubmed publisher
    b>Cryptosporidium parvum is a well-known waterborne and opportunistic intracellular protozoan parasite that causes diarrheal illness. In this study, we quantitatively investigated reduction of the infectivity of C...
  80. Johnson C, Gorla S, Kavitha M, Zhang M, Liu X, Striepen B, et al. Phthalazinone inhibitors of inosine-5'-monophosphate dehydrogenase from Cryptosporidium parvum. Bioorg Med Chem Lett. 2013;23:1004-7 pubmed publisher
    b>Cryptosporidium parvum (Cp) is a potential biowarfare agent and major cause of diarrhea and malnutrition. This protozoan parasite relies on inosine 5'-monophosphate dehydrogenase (IMPDH) for the production of guanine nucleotides...
  81. Benamrouz S, Guyot K, Gazzola S, Mouray A, Chassat T, Delaire B, et al. Cryptosporidium parvum infection in SCID mice infected with only one oocyst: qPCR assessment of parasite replication in tissues and development of digestive cancer. PLoS ONE. 2012;7:e51232 pubmed publisher
    ..SCID) mice were previously described as developing digestive adenocarcinoma after massive infection with Cryptosporidium parvum as soon as 45 days post-infection (P.I.)...
  82. Barakat F, McDonald V, Di Santo J, Korbel D. Roles for NK cells and an NK cell-independent source of intestinal gamma interferon for innate immunity to Cryptosporidium parvum infection. Infect Immun. 2009;77:5044-9 pubmed publisher
    A gamma interferon (IFN-gamma)-dependent innate immune response operates against the intestinal parasite Cryptosporidium parvum in T- and B-cell-deficient SCID mice...
  83. Artz J, Wernimont A, Allali Hassani A, Zhao Y, Amani M, Lin Y, et al. The Cryptosporidium parvum kinome. BMC Genomics. 2011;12:478 pubmed publisher
    ..the pursuit of anti-cryptosporidiosis targets and compounds, our study spans the classification of the Cryptosporidium parvum kinome and the structural and biochemical characterization of representatives from the CDPK family and a ..