coenzyme a transferases


Summary: Enzymes which transfer coenzyme A moieties from acyl- or acetyl-CoA to various carboxylic acceptors forming a thiol ester. Enzymes in this group are instrumental in ketone body metabolism and utilization of acetoacetate in mitochondria. EC 2.8.3.

Top Publications

  1. Oulton M, Amons R, Liang P, MacRae T. A 49 kDa microtubule cross-linking protein from Artemia franciscana is a coenzyme A-transferase. Eur J Biochem. 2003;270:4962-72 pubmed
    ..Probing of Western blots with an antibody against p49 revealed a cross-reactive protein in Drosophila that associated with microtubules, but to a lesser extent than did p49 from Artemia. ..
  2. Toyota C, Berthold C, Gruez A, Jonsson S, Lindqvist Y, Cambillau C, et al. Differential substrate specificity and kinetic behavior of Escherichia coli YfdW and Oxalobacter formigenes formyl coenzyme A transferase. J Bacteriol. 2008;190:2556-64 pubmed publisher
    ..Our findings illustrate the utility of structural homology in assigning enzyme function and raise the question of whether oxalate catabolism takes place in E. coli upon the up-regulation of the yfdXWUVE operon under acidic conditions...
  3. Alexeev D, Alexeeva M, Baxter R, Campopiano D, Webster S, Sawyer L. The crystal structure of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl-CoA-condensing enzyme. J Mol Biol. 1998;284:401-19 pubmed
    ..Knowledge of the three-dimensional structure of AONS will enable characterisation of the structural features of this enzyme sub-family that are responsible for this important type of reaction. ..
  4. Koga M, Tanaka H, Yomogida K, Nozaki M, Tsuchida J, Ohta H, et al. Isolation and characterization of a haploid germ cell-specific novel complementary deoxyribonucleic acid; testis-specific homologue of succinyl CoA:3-Oxo acid CoA transferase. Biol Reprod. 2000;63:1601-9 pubmed
    ..It was also detected in the midpiece of spermatozoa by immunohistochemistry. The results suggest that the scot-t protein plays important roles in the energy metabolism of spermatozoa. ..
  5. Onishi M, Yasunaga T, Tanaka H, Nishimune Y, Nozaki M. Gene structure and evolution of testicular haploid germ cell-specific genes, Oxct2a and Oxct2b. Genomics. 2004;83:647-57 pubmed
    ..Dot matrix and phylogenetic tree analyses demonstrated that multiple rounds of intrachromosomal gene conversion between the two loci occurred in each species independently. ..
  6. Hügler M, Fuchs G. Assaying for the 3-hydroxypropionate cycle of carbon fixation. Methods Enzymol. 2005;397:212-21 pubmed
    ..The fate of succinyl-CoA in Sulfolobales is at issue. Assays used to study the characteristic enzymes of this novel pathway in C. aurantiacus are reported...
  7. Kelly R, Bolitho M, Higgins D, Lu W, Ng W, Jeffrey P, et al. The Vibrio cholerae quorum-sensing autoinducer CAI-1: analysis of the biosynthetic enzyme CqsA. Nat Chem Biol. 2009;5:891-5 pubmed publisher
    ..cholerae responds to amino-CAI-1 as well as CAI-1, whereas other CAI-1 variants do not elicit a quorum-sensing response. Thus, both CAI-1 and amino-CAI-1 have potential as lead molecules in the development of an anticholera treatment. ..
  8. Fraser M, Hayakawa K, Brown W. Catalytic role of the conformational change in succinyl-CoA:3-oxoacid CoA transferase on binding CoA. Biochemistry. 2010;49:10319-28 pubmed publisher
    ..The results shed light on how the enzyme uses the binding energy for groups remote from the active center of CoA to destabilize atoms closer to the active center, leading to acceleration of the reaction by the enzyme...
  9. Louis P, Flint H. Development of a semiquantitative degenerate real-time pcr-based assay for estimation of numbers of butyryl-coenzyme A (CoA) CoA transferase genes in complex bacterial samples. Appl Environ Microbiol. 2007;73:2009-12 pubmed
    ..This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota. ..

More Information

Publications101 found, 100 shown here

  1. Wijkhuisen A, Djouadi F, Vilar J, Merlet Benichou C, Bastin J. Birth-related changes in energy metabolism enzymes and Na-K-ATPase in kidney proximal convoluted tubule cells. Am J Physiol. 1997;272:C787-93 pubmed
  2. MacDonald M, Longacre M, Langberg E, Tibell A, Kendrick M, Fukao T, et al. Decreased levels of metabolic enzymes in pancreatic islets of patients with type 2 diabetes. Diabetologia. 2009;52:1087-91 pubmed publisher
    ..Low levels of certain islet metabolic enzymes, especially mitochondrial enzymes, are associated with human type 2 diabetes. ..
  3. Zhang H, Boghigian B, Pfeifer B. Investigating the role of native propionyl-CoA and methylmalonyl-CoA metabolism on heterologous polyketide production in Escherichia coli. Biotechnol Bioeng. 2010;105:567-73 pubmed publisher
    ..Lastly, the effect of the ygfH deletion was tested in batch bioreactor cultures in which 6dEB titers improved from 206 to 527 mg/L. ..
  4. Charrier C, Duncan G, Reid M, Rucklidge G, Henderson D, Young P, et al. A novel class of CoA-transferase involved in short-chain fatty acid metabolism in butyrate-producing human colonic bacteria. Microbiology. 2006;152:179-85 pubmed publisher
    ..A2-183. This suggests strongly that the newly identified group of CoA-transferases described here plays a key role in butyrate formation in the human colon...
  5. Parthasarathy A, Buckel W, Smith D. On the thermodynamic equilibrium between (R)-2-hydroxyacyl-CoA and 2-enoyl-CoA. FEBS J. 2010;277:1738-46 pubmed publisher
    ..By examining the computational results, we arrive at an explanation of the substituent sensitivity and provide a prediction for the, as yet unmeasured, equilibrium involving 2-hydroxyisocaproyl-CoA. ..
  6. Murphy J, Mullins E, Kappock T. Functional Dissection of the Bipartite Active Site of the Class I Coenzyme A (CoA)-Transferase Succinyl-CoA:Acetate CoA-Transferase. Front Chem. 2016;4:23 pubmed publisher
    ..The ability of 2a to induce full active site closure furthermore suggests that it subverts a system used to impede inappropriate active site closure on unacylated CoA. ..
  7. Fischer R, Helms J, Dürre P. Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis. J Bacteriol. 1993;175:6959-69 pubmed
    ..mRNA studies revealed that ORF 5 formed a monocistronic operon and showed increased expression before onset of solventogenesis. ..
  8. Armengaud J, Timmis K, Wittich R. A functional 4-hydroxysalicylate/hydroxyquinol degradative pathway gene cluster is linked to the initial dibenzo-p-dioxin pathway genes in Sphingomonas sp. strain RW1. J Bacteriol. 1999;181:3452-61 pubmed
    ..A route for the mineralization of the growth substrates 3-hydroxydibenzofuran and 2-hydroxydibenzo-p-dioxin in Sphingomonas sp. strain RW1 thus suggests itself. ..
  9. Turko I, Marcondes S, Murad F. Diabetes-associated nitration of tyrosine and inactivation of succinyl-CoA:3-oxoacid CoA-transferase. Am J Physiol Heart Circ Physiol. 2001;281:H2289-94 pubmed
    ..Because diabetes results in profound derangements in myocardial substrate utilization, we suggest that SCOT tyrosine nitration is a contributing factor to this impairment in the diabetic heart. ..
  10. Cronan J. Remarkable structural variation within fatty acid megasynthases. Nat Chem Biol. 2006;2:232-4 pubmed
  11. Watson R, Dickson K. Enzyme activities support the use of liver lipid-derived ketone bodies as aerobic fuels in muscle tissues of active sharks. Physiol Biochem Zool. 2001;74:273-82 pubmed
    ..These results are consistent with the hypothesis that aerobic metabolism in muscle tissue of active sharks utilizes ketone bodies, and not fatty acids, derived from liver lipid stores. ..
  12. Louis P, McCrae S, Charrier C, Flint H. Organization of butyrate synthetic genes in human colonic bacteria: phylogenetic conservation and horizontal gene transfer. FEMS Microbiol Lett. 2007;269:240-7 pubmed
    ..The newly identified gene for butyryl-CoA CoA-transferase, which performs the final step in butyrate formation in most known human colonic bacteria, was not closely linked to these central pathway genes. ..
  13. Kaschabek S, Kuhn B, Müller D, Schmidt E, Reineke W. Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase. J Bacteriol. 2002;184:207-15 pubmed
  14. Göbel M, Kassel Cati K, Schmidt E, Reineke W. Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: cloning, characterization, and analysis of sequences encoding 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase. J Bacteriol. 2002;184:216-23 pubmed
    ..An incomplete ORF6 of 1,183 bp downstream of ORF5 and oriented in the opposite direction was found. The protein sequence deduced from ORF6 showed a putative AMP-binding domain signature. ..
  15. Mayr M, Yusuf S, Weir G, Chung Y, Mayr U, Yin X, et al. Combined metabolomic and proteomic analysis of human atrial fibrillation. J Am Coll Cardiol. 2008;51:585-94 pubmed publisher
  16. Fleck C, Brock M. Characterization of an acyl-CoA: carboxylate CoA-transferase from Aspergillus nidulans involved in propionyl-CoA detoxification. Mol Microbiol. 2008;68:642-56 pubmed publisher
  17. Merron S, Akhtar R. Management and communication problems in a patient with succinyl-CoA transferase deficiency in pregnancy and labour. Int J Obstet Anesth. 2009;18:280-3 pubmed publisher
    ..The needs of the woman's condition had to be balanced against the desire to minimise gastric volume in case emergency obstetric intervention was required. ..
  18. Junghare M, Spiteller D, Schink B. Enzymes involved in the anaerobic degradation of ortho-phthalate by the nitrate-reducing bacterium Azoarcus sp. strain PA01. Environ Microbiol. 2016;18:3175-88 pubmed publisher
    ..Further degradation of benzoyl-CoA proceeds analogous to the well-established anaerobic benzoyl-CoA degradation pathway of nitrate-reducing bacteria. ..
  19. Haney P, Bolinger L, Raefsky C, Patel M. Turnover of succinyl-CoA:3-oxoacid CoA-transferase in glioma and neuroblastoma cells. Specific influence of acetoacetate in neuroblastoma cells. Biochem J. 1984;224:67-74 pubmed
    ..025) in these cells on day 3, suggesting that substrate-mediated induction of enzyme synthesis is a mechanism of regulation of 3-oxoacid CoA-transferase. ..
  20. Cánovas M, Bernal V, Sevilla A, Iborra J. Role of wet experiment design in data generation: from in vivo to in silico and back. In Silico Biol. 2007;7:S3-16 pubmed
    ..The feed-back between the data generated and in silico modeling helps our understanding of the Escherichia coli expressed phenotype and permits new wet experiments to be designed to generate data concerning metabolic optimization. ..
  21. Bocan T, Krause B, Rosebury W, Lu X, Dagle C, Bak Mueller S, et al. The combined effect of inhibiting both ACAT and HMG-CoA reductase may directly induce atherosclerotic lesion regression. Atherosclerosis. 2001;157:97-105 pubmed
  22. Louis P, Flint H. Diversity, metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine. FEMS Microbiol Lett. 2009;294:1-8 pubmed publisher
    ..Populations of F. prausnitzii are reported to be decreased in Crohn's disease, for example, while populations of Roseburia relatives appear to be particularly sensitive to the diet composition in human volunteer studies...
  23. Hasan N, Longacre M, Seed Ahmed M, Kendrick M, Gu H, Ostenson C, et al. Lower succinyl-CoA:3-ketoacid-CoA transferase (SCOT) and ATP citrate lyase in pancreatic islets of a rat model of type 2 diabetes: knockdown of SCOT inhibits insulin release in rat insulinoma cells. Arch Biochem Biophys. 2010;499:62-8 pubmed publisher
    ..The results further support work that suggests mitochondrial pathways involving SCOT which supply acetoacetate for export to the cytosol are important for insulin secretion. ..
  24. Kim J, Darley D, Buckel W, Pierik A. An allylic ketyl radical intermediate in clostridial amino-acid fermentation. Nature. 2008;452:239-42 pubmed publisher
    ..The absence of radical generators such as coenzyme B12, S-adenosylmethionine or oxygen makes these enzymes unprecedented in biochemistry. ..
  25. Wentz A, d Avignon D, Weber M, Cotter D, Doherty J, Kerns R, et al. Adaptation of myocardial substrate metabolism to a ketogenic nutrient environment. J Biol Chem. 2010;285:24447-56 pubmed publisher
    ..These results indicate that ketotic nutrient environments engage mechanisms that curtail ketolytic capacity, controlling the utilization of ketone bodies in ketotic states. ..
  26. Coker S, Lloyd A, Mitchell E, Lewis G, Coker A, Shoolingin Jordan P. The high-resolution structure of pig heart succinyl-CoA:3-oxoacid coenzyme A transferase. Acta Crystallogr D Biol Crystallogr. 2010;66:797-805 pubmed publisher
    ..The binding orientation of one of the cosubstrates, acetoacetate, is suggested based on the glycerol binding and the possibility that this dynamic domain movement is of functional importance is discussed...
  27. Lantsman Y, Tan K, Morada M, Yarlett N. Biochemical characterization of a mitochondrial-like organelle from Blastocystis sp. subtype 7. Microbiology. 2008;154:2757-66 pubmed publisher
    ..Based upon the results of this study, the Blastocystis organelle falls into the category of a MLO...
  28. Gruez A, Roig Zamboni V, Valencia C, Campanacci V, Cambillau C. The crystal structure of the Escherichia coli YfdW gene product reveals a new fold of two interlaced rings identifying a wide family of CoA transferases. J Biol Chem. 2003;278:34582-6 pubmed publisher
  29. Hughes L, Smith P, Bonell S, Natt R, Wilson C, Rashid T, et al. Cross-reactivity between related sequences found in Acinetobacter sp., Pseudomonas aeruginosa, myelin basic protein and myelin oligodendrocyte glycoprotein in multiple sclerosis. J Neuroimmunol. 2003;144:105-15 pubmed
    ..Further work is required to evaluate the relevance of these cross-reactive antibodies to the neuropathology of MS...
  30. Kim J, Darley D, Selmer T, Buckel W. Characterization of (R)-2-hydroxyisocaproate dehydrogenase and a family III coenzyme A transferase involved in reduction of L-leucine to isocaproate by Clostridium difficile. Appl Environ Microbiol. 2006;72:6062-9 pubmed
    ..The dehydrogenase and the CoA transferase fit well into the proposed pathway of leucine reduction to isocaproate. ..
  31. Tummala S, Junne S, Papoutsakis E. Antisense RNA downregulation of coenzyme A transferase combined with alcohol-aldehyde dehydrogenase overexpression leads to predominantly alcohologenic Clostridium acetobutylicum fermentations. J Bacteriol. 2003;185:3644-53 pubmed
    ..The expression patterns of the CoAT genes (ctfA and ctfB) and aad were consistent with the overexpression of aad and asRNA downregulation of ctfB. ..
  32. Bernal V, Masdemont B, Arense P, Cánovas M, Iborra J. Redirecting metabolic fluxes through cofactor engineering: Role of CoA-esters pool during L(-)-carnitine production by Escherichia coli. J Biotechnol. 2007;132:110-7 pubmed
  33. Rebrin I, Bregere C, Kamzalov S, Gallaher T, Sohal R. Nitration of tryptophan 372 in succinyl-CoA:3-ketoacid CoA transferase during aging in rat heart mitochondria. Biochemistry. 2007;46:10130-44 pubmed
  34. Tsugawa H, Suzuki H, Nakagawa I, Nishizawa T, Saito Y, Suematsu M, et al. Alpha-ketoglutarate oxidoreductase, an essential salvage enzyme of energy metabolism, in coccoid form of Helicobacter pylori. Biochem Biophys Res Commun. 2008;376:46-51 pubmed publisher
    ..pylori, especially in cases infected with multiple antibiotic-resistant strains. ..
  35. van Grinsven K, van Hellemond J, Tielens A. Acetate:succinate CoA-transferase in the anaerobic mitochondria of Fasciola hepatica. Mol Biochem Parasitol. 2009;164:74-9 pubmed publisher
    ..It is homologous to the hydrogenosomal ASCT we earlier identified in Trichomonas vaginalis, but not to the ASCT present in the aerobic mitochondria of Trypanosoma brucei. ..
  36. Baetz A, Allison M. Purification and characterization of formyl-coenzyme A transferase from Oxalobacter formigenes. J Bacteriol. 1990;172:3537-40 pubmed
    ..0 mM and 29.6 mumols/min per mg for formyl-CoA with an excess of succinate. The maximum specific activity was 2.15 mumols of CoA transferred from formyl-CoA to oxalate per min per mg of protein. ..
  37. Yeh W, Ornston L. Evolutionarily homologous alpha 2 beta 2 oligomeric structures in beta-ketoadipate succinyl-CoA transferases from Acinetobacter calcoaceticus and Pseudomonas putida. J Biol Chem. 1981;256:1565-9 pubmed
    ..1.1.44) and beta-ketoadipate enol-lactone hydrolases (EC, enzymes that mediate consecutive reactions preceding the transferase step in the beta-ketoadipate pathway. ..
  38. D rre P, Fischer R, Kuhn A, Lorenz K, Schreiber W, St rzenhofecker B, et al. Solventogenic enzymes of Clostridium acetobutylicum: catalytic properties, genetic organization, and transcriptional regulation. FEMS Microbiol Rev. 1995;17:251-62 pubmed
    ..Although the signal that triggers the shift to solventogenesis has not yet been elucidated, recent investigations suggest a possible function of DNA supercoiling as a transcriptional sensor of the respective environmental stimuli...
  39. Morona R, Stroeher U, Karageorgos L, Brown M, Manning P. A putative pathway for biosynthesis of the O-antigen component, 3-deoxy-L-glycero-tetronic acid, based on the sequence of the Vibrio cholerae O1 rfb region. Gene. 1995;166:19-31 pubmed
    ..A biosynthetic pathway for the Vc O-antigen component 3-deoxy-L-glycero-tetronic acid, based on the enzymatic functions predicted for the RfbK, RfbL, RfbM, RfbN and RfbO proteins, is presented...
  40. Lloyd A, Shoolingin Jordan P. Dimeric pig heart succinate-coenzyme A transferase uses only one subunit to support catalysis. Biochemistry. 2001;40:2455-67 pubmed
    ..The possible structural basis for this catalytic asymmetry and its mechanistic implications are discussed. ..
  41. Marcondes S, Turko I, Murad F. Nitration of succinyl-CoA:3-oxoacid CoA-transferase in rats after endotoxin administration. Proc Natl Acad Sci U S A. 2001;98:7146-51 pubmed
    ..SCOT is a key enzyme for ketone body utilization. Thus, tyrosine nitration of the enzyme with sepsis or inflammation may explain the altered metabolism of ketone bodies present in these disorders. ..
  42. Selmer T, Willanzheimer A, Hetzel M. Propionate CoA-transferase from Clostridium propionicum. Cloning of gene and identification of glutamate 324 at the active site. Eur J Biochem. 2002;269:372-80 pubmed
    ..A high degree of structural similarity was observed between the arrangement of secondary structure elements in these proteins and glutaconate CoA-transferase from Acidaminococcus fermentans. ..
  43. Ricagno S, Jonsson S, Richards N, Lindqvist Y. Crystallization and preliminary crystallographic analysis of formyl-CoA tranferase from Oxalobacter formigenes. Acta Crystallogr D Biol Crystallogr. 2003;59:1276-7 pubmed
    ..44, c = 99.49 A. The asymmetric unit contains one dimer and the solvent content is 53%. Formyl-CoA transferase was crystallized both as the apoenzyme and as its complex with coenzyme A. ..
  44. Niggeweg R, Michael A, Martin C. Engineering plants with increased levels of the antioxidant chlorogenic acid. Nat Biotechnol. 2004;22:746-54 pubmed publisher
    ..Tomatoes with elevated CGA levels could be used in foods with specific benefits for human health...
  45. Longo N, Fukao T, Singh R, Pasquali M, Barrios R, Kondo N, et al. Succinyl-CoA:3-ketoacid transferase (SCOT) deficiency in a new patient homozygous for an R217X mutation. J Inherit Metab Dis. 2004;27:691-2 pubmed
    ..Before confirmation of diagnosis, daily dialysis was the only mechanism by which to normalize her persistent metabolic acidosis of unknown aetiology. ..
  46. Kosaka T, Uchiyama T, Ishii S, Enoki M, Imachi H, Kamagata Y, et al. Reconstruction and regulation of the central catabolic pathway in the thermophilic propionate-oxidizing syntroph Pelotomaculum thermopropionicum. J Bacteriol. 2006;188:202-10 pubmed
    ..Results suggest that fumarase is the central metabolic switch controlling the metabolic flow and energy conservation in this syntroph...
  47. Yasuda K, Jojima T, Suda M, Okino S, Inui M, Yukawa H. Analyses of the acetate-producing pathways in Corynebacterium glutamicum under oxygen-deprived conditions. Appl Microbiol Biotechnol. 2007;77:853-60 pubmed
    ..These results indicate that PTA-ACK as well as CTF is involved in acetate production in C. glutamicum. This study provided basic information to reduce acetate production under oxygen-deprived conditions. ..
  48. Nau K, Fromme T, Meyer C, von Praun C, Heldmaier G, Klingenspor M. Brown adipose tissue specific lack of uncoupling protein 3 is associated with impaired cold tolerance and reduced transcript levels of metabolic genes. J Comp Physiol B. 2008;178:269-77 pubmed
    ..This phenotype is associated with a global decrease in expression of metabolic genes but not of uncoupling protein 1. These data implicate that Ucp3 is necessary to sustain high metabolic rates in brown adipose tissue. ..
  49. Turroni S, Bendazzoli C, Dipalo S, Candela M, Vitali B, Gotti R, et al. Oxalate-degrading activity in Bifidobacterium animalis subsp. lactis: impact of acidic conditions on the transcriptional levels of the oxalyl coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes. Appl Environ Microbiol. 2010;76:5609-20 pubmed publisher
    ..animalis subsp. lactis as a promising adjunct for the prophylaxis and management of oxalate-related kidney disease. ..
  50. Parales R, Harwood C. Characterization of the genes encoding beta-ketoadipate: succinyl-coenzyme A transferase in Pseudomonas putida. J Bacteriol. 1992;174:4657-66 pubmed
    ..Conserved functional groups important to the catalytic activity of CoA transferases were also identified...
  51. Zołnierowicz S, Scisłowski P, Swierczynski J, Zelewski L. Acetoacetate utilization by human placental mitochondria. Placenta. 1984;5:271-6 pubmed
    ..It is proposed that acetoacetate utilization by placental mitochondria proceeds via the conversion to acetoacetyl-CoA catalysed by 3-oxoacid-CoA transferase, and then to acetyl-CoA, catalysed by acetoacetyl-CoA thiolase. ..
  52. Steinmann D, K plin R, P hler A, Niehaus K. Xanthomonas campestris pv. campestris lpsI and lpsJ genes encoding putative proteins with sequence similarity to the alpha- and beta-subunits of 3-oxoacid CoA-transferases are involved in LPS biosynthesis. Arch Microbiol. 1997;168:441-7 pubmed
    ..The monosaccharide composition of the exopolysaccharide xanthan produced by lpsI and lpsJ mutants remained unchanged...
  53. Korolev S, Koroleva O, Petterson K, Gu M, Collart F, Dementieva I, et al. Autotracing of Escherichia coli acetate CoA-transferase alpha-subunit structure using 3.4 A MAD and 1.9 A native data. Acta Crystallogr D Biol Crystallogr. 2002;58:2116-21 pubmed
    ..4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal. ..
  54. Tummala S, Welker N, Papoutsakis E. Design of antisense RNA constructs for downregulation of the acetone formation pathway of Clostridium acetobutylicum. J Bacteriol. 2003;185:1923-34 pubmed
    ..These results show that CoAT is the rate-limiting enzyme in acetone formation and strengthen the hypothesis that the component/nucleotide ratio is a predictive indicator of asRNA effectiveness. ..
  55. O DONNELL J, Laffan M. Dissociation of ABH antigen expression from von Willebrand factor synthesis in endothelial cell lines. Br J Haematol. 2003;121:928-31 pubmed
    ..Further studies demonstrated H structures were not present on EAhy926-derived VWF, despite the fact that H antigen is constitutively expressed by these cells. ..
  56. Jonsson S, Ricagno S, Lindqvist Y, Richards N. Kinetic and mechanistic characterization of the formyl-CoA transferase from Oxalobacter formigenes. J Biol Chem. 2004;279:36003-12 pubmed
    ..The catalytic mechanism of formyl-CoA transferase is therefore established and is almost certainly employed by all other members of the Class III CoA-transferase family...
  57. Hokama S, Toma C, Iwanaga M, Morozumi M, Sugaya K, Ogawa Y. Oxalate-degrading Providencia rettgeri isolated from human stools. Int J Urol. 2005;12:533-8 pubmed
    ..Oxalate-degrading bacteria are thought to metabolize intestinal oxalate and thus decrease the urinary excretion of oxalate by reducing its intestinal absorption...
  58. Loncaric C, Ward A, Walker K. Expression of an acetyl-CoA synthase and a CoA-transferase in Escherichia coli to produce modified taxanes in vivo. Biotechnol J. 2007;2:266-74 pubmed
    ..6% conversion to 10-butyrylbaccatin III was observed compared to approximately 0.6% conversion when no exogenous butyrate was supplied. ..
  59. Lam P, Yin F, Hamilton R, Boveris A, Cadenas E. Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production. Free Radic Res. 2009;43:431-9 pubmed publisher
  60. Jacob U, Mack M, Clausen T, Huber R, Buckel W, Messerschmidt A. Glutaconate CoA-transferase from Acidaminococcus fermentans: the crystal structure reveals homology with other CoA-transferases. Structure. 1997;5:415-26 pubmed
    ..We initiated this project to gain further insight into the function of this enzyme and the structural basis for the characteristics of CoA-transferases...
  61. ter Kuile B. Regulation and adaptation of glucose metabolism of the parasitic protist Leishmania donovani at the enzyme and mRNA levels. J Bacteriol. 1999;181:4863-72 pubmed
    ..donovani and other species suggests that the energy metabolism of L. donovani is inefficient but is well suited to the environmental challenges that it encounters during residence in the sandfly, its insect vector. ..
  62. Thormann K, Dürre P. Orf5/SolR: a transcriptional repressor of the sol operon of Clostridium acetobutylicum?. J Ind Microbiol Biotechnol. 2001;27:307-13 pubmed
    ..Based on these results Orf5 (SolR) appears not to act as a transcriptional repressor in C. acetobutylicum, but instead may be an enzyme involved in glycosylation or deglycosylation. ..
  63. Starck J, Kallenius G, Marklund B, Andersson D, Akerlund T. Comparative proteome analysis of Mycobacterium tuberculosis grown under aerobic and anaerobic conditions. Microbiology. 2004;150:3821-9 pubmed
    ..Some protein spots were found to be proteolytic fragments, e.g. HspX and GroEL2. These data suggest that M. tuberculosis induces expression of about 1 % of its genes in response to dormancy. ..
  64. El Midaoui A, Chiasson J, Tancrède G, Nadeau A. Physical training reverses defect in 3-ketoacid CoA-transferase activity in skeletal muscle of diabetic rats. Am J Physiol Endocrinol Metab. 2005;288:E748-52 pubmed
  65. Fukao T, Sakurai S, Rolland M, Zabot M, Schulze A, Yamada K, et al. A 6-bp deletion at the splice donor site of the first intron resulted in aberrant splicing using a cryptic splice site within exon 1 in a patient with succinyl-CoA: 3-Ketoacid CoA transferase (SCOT) deficiency. Mol Genet Metab. 2006;89:280-2 pubmed
  66. Friedmann S, Alber B, Fuchs G. Properties of succinyl-coenzyme A:D-citramalate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus. J Bacteriol. 2006;188:6460-8 pubmed
    ..It is proposed that the CoA transferase and the lyase catalyze the last two steps in the glyoxylate assimilation route...
  67. Tammam S, Rochet J, Fraser M. Identification of the cysteine residue exposed by the conformational change in pig heart succinyl-CoA:3-ketoacid coenzyme A transferase on binding coenzyme A. Biochemistry. 2007;46:10852-63 pubmed
    ..Modeling this conformational change has led to the proposal of a new mechanism for catalysis by SCOT. ..
  68. Macieira S, Zhang J, Velarde M, Buckel W, Messerschmidt A. Crystal structure of 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum. Biol Chem. 2009;390:1251-63 pubmed publisher
    ..4-HB-CoAT from C. aminobutyricum represents the only functionally characterized 4-HB-CoAT present in the structural database. ..
  69. Lin T, Bridger W. Sequence of a cDNA clone encoding pig heart mitochondrial CoA transferase. J Biol Chem. 1992;267:975-8 pubmed
    ..We have identified the site of proteolysis, and analysis of the sequence in its vicinity suggests that the polypeptide may fold into two domains connected by a highly hydrophilic bridge. ..
  70. Valentin H, Reiser S, Gruys K. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) formation from gamma-aminobutyrate and glutamate. Biotechnol Bioeng. 2000;67:291-9 pubmed
    ..Functionality of the biosynthetic pathway for copolymer production was demonstrated through feeding experiments using various carbon sources that supplied different precursors within the 4HB-CoA biosynthetic pathway. ..
  71. Duncan S, Barcenilla A, Stewart C, Pryde S, Flint H. Acetate utilization and butyryl coenzyme A (CoA):acetate-CoA transferase in butyrate-producing bacteria from the human large intestine. Appl Environ Microbiol. 2002;68:5186-90 pubmed
    ..These results demonstrate that different functionally distinct groups of butyrate-producing bacteria are present in the human large intestine...
  72. Benani A, Vol C, Heurtaux T, Asensio C, Dauca M, Lapicque F, et al. Up-regulation of fatty acid metabolizing-enzymes mRNA in rat spinal cord during persistent peripheral local inflammation. Eur J Neurosci. 2003;18:1904-14 pubmed
    ..These findings suggest that the fatty metabolism is stimulated in the CNS during a chronic pain state. ..
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    ..Each protomer binds a single CoA molecule and these CoA-binding sites are distant from one another in the dimer. ..
  74. Brock M, Buckel W. On the mechanism of action of the antifungal agent propionate. Eur J Biochem. 2004;271:3227-41 pubmed
    ..A possible toxicity of propionyl-CoA for humans in diseases such as propionic acidaemia and methylmalonic aciduria is also discussed. ..
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    ..Response current increased linearly with increase in propionate concentration from 10 microM to 100 microM. The detection limit was 10 microM propionate. ..
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    ..Fed-batch cultures were performed to produce P(3HB-co-LA) copolymers having 9-64 mol% of lactate, and their molecular weights, thermal properties, and melt flow properties were determined. ..
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    ..These studies suggest that WR201 should be further investigated as a vaccine to prevent human brucellosis. ..
  79. Fukao T, Mitchell G, Song X, Nakamura H, Kassovska Bratinova S, Orii K, et al. Succinyl-CoA:3-ketoacid CoA transferase (SCOT): cloning of the human SCOT gene, tertiary structural modeling of the human SCOT monomer, and characterization of three pathogenic mutations. Genomics. 2000;68:144-51 pubmed
    ..Interestingly, GS05 had the mildest clinical course reported to date and detectable levels of SCOT protein in fibroblasts. ..
  80. Sawai M, Yashiro M, Nishiguchi Y, Ohira M, Hirakawa K. Growth-inhibitory effects of the ketone body, monoacetoacetin, on human gastric cancer cells with succinyl-CoA: 3-oxoacid CoA-transferase (SCOT) deficiency. Anticancer Res. 2004;24:2213-7 pubmed
    ..SCOT mRNA was expressed in MKN-45, MKN-74 and normal fibroblasts, but not in MKN-28 or OCUM-2M. Parenteral nutrition with MAA may provide preferential energy for patients with some types of gastric cancer with SCOT deficiency. ..
  81. Stenmark P, Gurmu D, Nordlund P. Crystal structure of CaiB, a type-III CoA transferase in carnitine metabolism. Biochemistry. 2004;43:13996-4003 pubmed
    ..This is the first observation of a domain movement in the type-III CoA transferase family and can play an important role in coupling substrate binding to initiation of the catalytic reaction. ..
  82. Rajashekhara E, Hosoda A, Sode K, Ikenaga H, Watanabe K. Volatile fatty acid-sensing system involving coenzyme-A transferase. Biotechnol Prog. 2006;22:334-7 pubmed
    ..We demonstrated its utility by measuring propionate concentrations in serum and fermentation samples. Results suggest that our biosensing system is applicable to the detection of propionate in medical and fermentation samples. ..
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    ..Here, we describe how we performed the gene trap screen and characterized the gene trap insertions and will discuss the outcome of the pilot screen. ..
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    ..On the other hand, SCOT mRNA with exons 6 and 7 is a minor transcript but it retains the reading-frame and is stable in cytosol. As a result, the latter mRNA is more abundant under steady-state conditions as compared to the former mRNA. ..
  86. Jossek R, Steinbuchel A. In vitro synthesis of poly(3-hydroxybutyric acid) by using an enzymatic coenzyme A recycling system. FEMS Microbiol Lett. 1998;168:319-24 pubmed
    ..In this way the in vitro synthesis of P(3HB) became independent of the consumption of the expensive coenzyme A. By this procedure a handy system is available to produce in vitro PHA on a semipreparative scale. ..
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    ..The small acetate molecule in the substrate binding pocket may activate a water molecule for hydrolysis of the nearby enzyme-CoA thiol ester. ..
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    ..As suggested by kinetic analysis, the reaction mechanism of enzymes of this family apparently involves formation of a ternary complex between the enzyme and the two substrates. ..
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    ..These observations support the hypothesis that reductions in KGDHC may impair the adaptability of the brain and contribute to the pathogenesis of neurodegenerative diseases. ..
  91. O DONNELL J, Boulton F, Manning R, Laffan M. Amount of H antigen expressed on circulating von Willebrand factor is modified by ABO blood group genotype and is a major determinant of plasma von Willebrand factor antigen levels. Arterioscler Thromb Vasc Biol. 2002;22:335-41 pubmed
    ..In contrast, among group A(1)A(1) and A(1)O(1) individuals, there was no relationship between AvWF and plasma vWF levels. These findings suggest that it is H antigen expression that mediates the ABO effect on plasma vWF concentration. ..
  92. Fukao T, Shintaku H, Kusubae R, Zhang G, Nakamura K, Kondo M, et al. Patients homozygous for the T435N mutation of succinyl-CoA:3-ketoacid CoA Transferase (SCOT) do not show permanent ketosis. Pediatr Res. 2004;56:858-63 pubmed
    ..In SCOT-deficient patients retaining some residual activity, permanent ketosis may be absent. ..