myosin light chain kinase

Summary

Summary: An enzyme that phosphorylates myosin light chains in the presence of ATP to yield myosin-light chain phosphate and ADP, and requires calcium and CALMODULIN. The 20-kDa light chain is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors. The enzyme plays a central role in the regulation of smooth muscle contraction.

Top Publications

  1. Clayburgh D, Barrett T, Tang Y, Meddings J, Van Eldik L, Watterson D, et al. Epithelial myosin light chain kinase-dependent barrier dysfunction mediates T cell activation-induced diarrhea in vivo. J Clin Invest. 2005;115:2702-15 pubmed
    ..Genetic knockout of long myosin light chain kinase (MLCK) or treatment of wild-type mice with a highly specific peptide MLCK inhibitor prevented epithelial ..
  2. Kudryashov D, Stepanova O, Vilitkevich E, Nikonenko T, Nadezhdina E, Shanina N, et al. Myosin light chain kinase (210 kDa) is a potential cytoskeleton integrator through its unique N-terminal domain. Exp Cell Res. 2004;298:407-17 pubmed
    Recently discovered 210-kDa myosin light chain kinase (MLCK-210) is identical to 108-130 kDa MLCK, the principal regulator of the myosin II molecular motor, except for the presence of a unique amino terminal extension...
  3. Fazal F, Gu L, Ihnatovych I, Han Y, Hu W, Antic N, et al. Inhibiting myosin light chain kinase induces apoptosis in vitro and in vivo. Mol Cell Biol. 2005;25:6259-66 pubmed
    ..precedes caspase activation when cells are stimulated with a proapoptotic agent or when myosin light chain kinase (MLCK) is inhibited pharmacologically or by microinjecting an inhibitory antibody to MLCK...
  4. Clayburgh D, Rosen S, Witkowski E, Wang F, Blair S, Dudek S, et al. A differentiation-dependent splice variant of myosin light chain kinase, MLCK1, regulates epithelial tight junction permeability. J Biol Chem. 2004;279:55506-13 pubmed
    ..These results demonstrate unique physiologically relevant patterns of expression and subcellular localization for long MLCK isoforms and show that MLCK1 is the isoform responsible for tight junction regulation in absorptive enterocytes. ..
  5. Ait Belgnaoui A, Bradesi S, Fioramonti J, Theodorou V, Bueno L. Acute stress-induced hypersensitivity to colonic distension depends upon increase in paracellular permeability: role of myosin light chain kinase. Pain. 2005;113:141-7 pubmed
    ..of epithelial cell contraction in the stress-induced increased permeability and hyperalgesia, using a myosin light chain kinase inhibitor (ML-7)...
  6. Shen Q, Rigor R, Pivetti C, Wu M, Yuan S. Myosin light chain kinase in microvascular endothelial barrier function. Cardiovasc Res. 2010;87:272-80 pubmed publisher
    ..b>Myosin light chain kinase (MLCK) plays an important role in maintaining the equilibrium by phosphorylating myosin light chain (MLC)..
  7. Lucero A, Stack C, Bresnick A, Shuster C. A global, myosin light chain kinase-dependent increase in myosin II contractility accompanies the metaphase-anaphase transition in sea urchin eggs. Mol Biol Cell. 2006;17:4093-104 pubmed
    ..The initial rise in contractility required myosin light chain kinase but not Rho-kinase, but both signaling pathways were required for successful cytokinesis...
  8. Kim B, Jeon J, Kim S, So I. Role of calmodulin and myosin light chain kinase in the activation of carbachol-activated cationic current in murine ileal myocytes. Can J Physiol Pharmacol. 2007;85:1254-62 pubmed
    We investigated the effect of calmodulin (CaM) and myosin light chain kinase (MLCK) on murine ileal myocytes using the whole-cell patch-clamp technique...
  9. Lee J, Kim M, Ho W, Lee S. Presynaptic release probability and readily releasable pool size are regulated by two independent mechanisms during posttetanic potentiation at the calyx of Held synapse. J Neurosci. 2008;28:7945-53 pubmed publisher
    ..In contrast, myosin light chain kinase (MLCK) inhibitors and blebbistatin reduced the posttetanic increase in RRP size with no effect on the ..

More Information

Publications62

  1. Takashima S. Phosphorylation of myosin regulatory light chain by myosin light chain kinase, and muscle contraction. Circ J. 2009;73:208-13 pubmed
    ..Among these, I have focused on myosin light chain kinase (MLCK) in this review...
  2. Helfman D, Pawlak G. Myosin light chain kinase and acto-myosin contractility modulate activation of the ERK cascade downstream of oncogenic Ras. J Cell Biochem. 2005;95:1069-80 pubmed
    ..e., myosin light chain kinase and Rho kinase, in oncogenic Ki-Ras-induced signaling...
  3. Owens S, Graham W, Siccardi D, Turner J, Mrsny R. A strategy to identify stable membrane-permeant peptide inhibitors of myosin light chain kinase. Pharm Res. 2005;22:703-9 pubmed
    A peptide inhibitor of myosin light chain kinase (MLCK), termed membrane permeant inhibitor of myosin light chain kinase (PIK), has previously been demonstrated to correct paracellular barrier defects associated with in vitro cell models ..
  4. Wadgaonkar R, Linz McGillem L, Zaiman A, Garcia J. Endothelial cell myosin light chain kinase (MLCK) regulates TNFalpha-induced NFkappaB activity. J Cell Biochem. 2005;94:351-64 pubmed
    ..Together, these novel observations indicate that TNFalpha-induced cytoskeletal rearrangement driven by MLCK activity is necessary for TNFalpha-dependent NFkappaB activation and amplification of pro-survival signals. ..
  5. Gao L, Grant A, Halder I, Brower R, Sevransky J, Maloney J, et al. Novel polymorphisms in the myosin light chain kinase gene confer risk for acute lung injury. Am J Respir Cell Mol Biol. 2006;34:487-95 pubmed
    The genetic basis of acute lung injury (ALI) is poorly understood. The myosin light chain kinase (MYLK) gene encodes the nonmuscle myosin light chain kinase isoform, a multifunctional protein involved in the inflammatory response (..
  6. Wong R, Fabian L, Forer A, Brill J. Phospholipase C and myosin light chain kinase inhibition define a common step in actin regulation during cytokinesis. BMC Cell Biol. 2007;8:15 pubmed
    ..Furthermore, treatment with the myosin light chain kinase (MLCK) inhibitor ML-7 caused cleavage furrow regression and loss of both F-actin and phosphorylated ..
  7. Herring B, El Mounayri O, Gallagher P, Yin F, Zhou J. Regulation of myosin light chain kinase and telokin expression in smooth muscle tissues. Am J Physiol Cell Physiol. 2006;291:C817-27 pubmed
    ..The mylk1 gene encodes at least four protein products: two isoforms of the 220-kDa myosin light chain kinase (MLCK), a 130-kDa MLCK, and telokin...
  8. Luh C, Kuhlmann C, Ackermann B, Timaru Kast R, Luhmann H, Behl C, et al. Inhibition of myosin light chain kinase reduces brain edema formation after traumatic brain injury. J Neurochem. 2010;112:1015-25 pubmed publisher
  9. Shimizu S, Yoshida T, Wakamori M, Ishii M, Okada T, Takahashi M, et al. Ca2+-calmodulin-dependent myosin light chain kinase is essential for activation of TRPC5 channels expressed in HEK293 cells. J Physiol. 2006;570:219-35 pubmed
    ..ML-9 and wortmannin, inhibitors of Ca(2+)-calmodulin-dependent myosin light chain kinase (MLCK), and the expression of a dominant-negative mutant of MLCK inhibited the TRPC5 channel activity, ..
  10. Zhou X, Liu Y, You J, Zhang H, Zhang X, Ye L. Myosin light-chain kinase contributes to the proliferation and migration of breast cancer cells through cross-talk with activated ERK1/2. Cancer Lett. 2008;270:312-27 pubmed publisher
  11. Komatsu S, Ikebe M. ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts. J Cell Biol. 2004;165:243-54 pubmed
    ..These results suggest that ZIP kinase is critical for myosin phosphorylation and necessary for cell motile processes in mammalian fibroblasts. ..
  12. Chou C, Christensen B, Frische S, Vorum H, Desai R, Hoffert J, et al. Non-muscle myosin II and myosin light chain kinase are downstream targets for vasopressin signaling in the renal collecting duct. J Biol Chem. 2004;279:49026-35 pubmed
    ..water permeability response is mediated in part through activation of the calcium/calmodulin-dependent myosin light chain kinase (MLCK) and regulation of non-muscle myosin II...
  13. Levinson H, Moyer K, Saggers G, Ehrlich H. Calmodulin-myosin light chain kinase inhibition changes fibroblast-populated collagen lattice contraction, cell migration, focal adhesion formation, and wound contraction. Wound Repair Regen. 2004;12:505-11 pubmed
    ..pathways can perform that phosphorylation, the focus here is calcium saturated calmodulin dependent -myosin light chain kinase (CaM-MLCK)...
  14. Christie J, Ma S, Aplenc R, Li M, Lanken P, Shah C, et al. Variation in the myosin light chain kinase gene is associated with development of acute lung injury after major trauma. Crit Care Med. 2008;36:2794-800 pubmed
    Single nucleotide polymorphisms in the myosin light chain kinase (MYLK) gene have been implicated in the risk of sepsis-related acute lung injury and asthma...
  15. Goldberg J, Wolpin E, Bosgraaf L, Clarkson B, Van Haastert P, Smith J. Myosin light chain kinase A is activated by cGMP-dependent and cGMP-independent pathways. FEBS Lett. 2006;580:2059-64 pubmed publisher
    ..We show that myosin light chain kinase A (MLCK-A) is responsible for RLC phosphorylation during chemotaxis, and that MLCK-A itself is ..
  16. Somlyo A, Wang H, Choudhury N, Khromov A, Majesky M, Owens G, et al. Myosin light chain kinase knockout. J Muscle Res Cell Motil. 2004;25:241-2 pubmed
  17. Matsumura F. Regulation of myosin II during cytokinesis in higher eukaryotes. Trends Cell Biol. 2005;15:371-7 pubmed
    ..I discuss proposed functions for MLCK, ROCK, citron kinase and myosin phosphatase during cytokinesis and consider the possibility of a link between these molecules and the signals transmitted by the mitotic spindle. ..
  18. Connell L, Helfman D. Myosin light chain kinase plays a role in the regulation of epithelial cell survival. J Cell Sci. 2006;119:2269-81 pubmed
    ..In this study we investigated the role of acto-myosin contractility, and its main regulators, i.e. myosin light chain kinase (MLCK) and Rho-kinase (ROCK) in cell survival in normal and Ras-transformed MCF-10A epithelial cells...
  19. Mabuchi Y, Mabuchi K, Stafford W, Grabarek Z. Modular structure of smooth muscle Myosin light chain kinase: hydrodynamic modeling and functional implications. Biochemistry. 2010;49:2903-17 pubmed publisher
    Smooth muscle myosin light chain kinase (smMLCK) is a calcium-calmodulin complex-dependent enzyme that activates contraction of smooth muscle...
  20. Haorah J, Heilman D, Knipe B, Chrastil J, Leibhart J, Ghorpade A, et al. Ethanol-induced activation of myosin light chain kinase leads to dysfunction of tight junctions and blood-brain barrier compromise. Alcohol Clin Exp Res. 2005;29:999-1009 pubmed
    ..Cytoskeletal alterations (MLC) and TJ changes (occludin and claudin-5 phosphorylation) result in BBB impairment (decrease in TEER). TJ compromise is associated with increased monocyte migration across the BBB. ..
  21. Matson S, Markoulaki S, Ducibella T. Antagonists of myosin light chain kinase and of myosin II inhibit specific events of egg activation in fertilized mouse eggs. Biol Reprod. 2006;74:169-76 pubmed
    ..A well-established Ca(2+)/CAM-dependent enzyme is myosin light chain kinase (MYLK2), the downstream target of which is myosin II, an isoform of myosin known to be important in ..
  22. Olsson M, Patel J, Fitzsimons D, Walker J, Moss R. Basal myosin light chain phosphorylation is a determinant of Ca2+ sensitivity of force and activation dependence of the kinetics of myocardial force development. Am J Physiol Heart Circ Physiol. 2004;287:H2712-8 pubmed
    ..58% of total was achieved by incubating BDM-treated trabeculae in solution containing smooth muscle myosin light chain kinase, calmodulin, and Ca2+ (i.e., MLCK treatment)...
  23. Moitra J, Evenoski C, Sammani S, Wadgaonkar R, Turner J, Ma S, et al. A transgenic mouse with vascular endothelial over-expression of the non-muscle myosin light chain kinase-2 isoform is susceptible to inflammatory lung injury: role of sexual dimorphism and age. Transl Res. 2008;151:141-53 pubmed publisher
    ..These mice express a nonmuscle isoform of the myosin light chain kinase gene (nmMLCK2) targeted to the endothelium...
  24. Srinivasan G, Kim J, von Gersdorff H. The pool of fast releasing vesicles is augmented by myosin light chain kinase inhibition at the calyx of Held synapse. J Neurophysiol. 2008;99:1810-24 pubmed publisher
    ..Here we describe the effects of blocking myosin light chain kinase (MLCK), a cytoskeletal regulatory protein thought to be involved in myosin-mediated vesicle transport, ..
  25. He W, Peng Y, Zhang W, Lv N, Tang J, Chen C, et al. Myosin light chain kinase is central to smooth muscle contraction and required for gastrointestinal motility in mice. Gastroenterology. 2008;135:610-20 pubmed publisher
    ..smooth muscle contraction with or without myosin regulatory light chain (RLC) phosphorylation by myosin light chain kinase (MLCK)...
  26. Han Y, de Lanerolle P. Naturally extended CT . AG repeats increase H-DNA structures and promoter activity in the smooth muscle myosin light chain kinase gene. Mol Cell Biol. 2008;28:863-72 pubmed
    ..In support of this, we found (CT)(22) . (AG)(22) repeats in the promoter of smooth muscle myosin light chain kinase (smMLCK), a key regulator of vascular smooth muscle function...
  27. Batchelder E, Thomas Virnig C, Hardin J, White J. Cytokinesis is not controlled by calmodulin or myosin light chain kinase in the Caenorhabditis elegans early embryo. FEBS Lett. 2007;581:4337-41 pubmed
    ..The calmodulin effector myosin light chain kinase (MLCK) can also phosphorylate mRLC and is widely regarded as a candidate for redundant function with ..
  28. Yang C, Chen H, Chen C, Yu W, Zhang W, Peng Y, et al. Microfilament-binding properties of N-terminal extension of the isoform of smooth muscle long myosin light chain kinase. Cell Res. 2006;16:367-76 pubmed
    ..The ability of short myosin light chain kinase (S-MLCK) to bind F-actin is structurally attributed to the DFRXXL regions in its N-terminus...
  29. Jordan R, Lemke E, Klingauf J. Visualization of synaptic vesicle movement in intact synaptic boutons using fluorescence fluctuation spectroscopy. Biophys J. 2005;89:2091-102 pubmed
    ..Inhibition of the myosin light chain kinase slows down vesicle mobility 10-fold, whereas other kinases like protein kinase C (PKC), A (PKA), and ..
  30. Utech M, Ivanov A, Samarin S, Bruewer M, Turner J, Mrsny R, et al. Mechanism of IFN-gamma-induced endocytosis of tight junction proteins: myosin II-dependent vacuolarization of the apical plasma membrane. Mol Biol Cell. 2005;16:5040-52 pubmed
    ..formation of VACs and endocytosis of TJ proteins was mediated by Rho-associated kinase (ROCK) but not myosin light chain kinase (MLCK)...
  31. Moriez R, Salvador Cartier C, Theodorou V, Fioramonti J, Eutamene H, Bueno L. Myosin light chain kinase is involved in lipopolysaccharide-induced disruption of colonic epithelial barrier and bacterial translocation in rats. Am J Pathol. 2005;167:1071-9 pubmed
    ..triggers BT, colonic inflammation, visceral pain, and sickness behavior and to evaluate the role of myosin light chain kinase (MLCK) in colonocyte cytoskeleton contraction...
  32. Heller W, Krueger J, Trewhella J. Further insights into calmodulin-myosin light chain kinase interaction from solution scattering and shape restoration. Biochemistry. 2003;42:10579-88 pubmed
    ..We have gained new insight into the interactions between the second-messenger protein calmodulin (CaM) and myosin light chain kinase from skeletal muscle (skMLCK) using small-angle solution scattering and shape restoration...
  33. Wei B, da Rocha Dias S, Wang H, Rudd C. CTL-associated antigen-4 ligation induces rapid T cell polarization that depends on phosphatidylinositol 3-kinase, Vav-1, Cdc42, and myosin light chain kinase. J Immunol. 2007;179:400-8 pubmed
    ..Our results demonstrate that CTLA-4 is a potent activator T cell polarization needed for motility, and this process involves specific set of signaling proteins that might contribute to coreceptor regulation of T cell function. ..
  34. Afonso P, Ozden S, Prevost M, Schmitt C, Seilhean D, Weksler B, et al. Human blood-brain barrier disruption by retroviral-infected lymphocytes: role of myosin light chain kinase in endothelial tight-junction disorganization. J Immunol. 2007;179:2576-83 pubmed
    ..These changes could be prevented by inhibition of the NF-kappaB pathway or of myosin light chain kinase activity...
  35. Brown M, Adyshev D, Bindokas V, Moitra J, Garcia J, Dudek S. Quantitative distribution and colocalization of non-muscle myosin light chain kinase isoforms and cortactin in human lung endothelium. Microvasc Res. 2010;80:75-88 pubmed publisher
    ..in myosin light chain (MLC) phosphorylation, catalyzed by Ca(2+)/calmodulin-dependent non-muscle myosin light chain kinase variants (nmMLCK1- and -2), as well as association of nmMLCK with the actin-binding protein, cortactin...
  36. Raina H, Zacharia J, Li M, Wier W. Activation by Ca2+/calmodulin of an exogenous myosin light chain kinase in mouse arteries. J Physiol. 2009;587:2599-612 pubmed publisher
    Activation of myosin light chain kinase (MLCK) and other kinases was studied in the arteries of transgenic mice that express an optical fluorescence resonance energy transfer (FRET) MLCK activity biosensor...
  37. Costantini T, Loomis W, Putnam J, Kroll L, Eliceiri B, Baird A, et al. Pentoxifylline modulates intestinal tight junction signaling after burn injury: effects on myosin light chain kinase. J Trauma. 2009;66:17-24; discussion 24-5 pubmed publisher
    ..b>Myosin light chain kinase (MLCK), a tight junction protein involved in the regulation of barrier function, increases intestinal ..
  38. Blair S, Kane S, Clayburgh D, Turner J. Epithelial myosin light chain kinase expression and activity are upregulated in inflammatory bowel disease. Lab Invest. 2006;86:191-201 pubmed
    ..In vitro studies show that cytokine-induced epithelial barrier dysfunction can be mediated by increased myosin light chain kinase (MLCK) expression and subsequent myosin II regulatory light chain (MLC) phosphorylation...
  39. Tohtong R, Phattarasakul K, Jiraviriyakul A, Sutthiphongchai T. Dependence of metastatic cancer cell invasion on MLCK-catalyzed phosphorylation of myosin regulatory light chain. Prostate Cancer Prostatic Dis. 2003;6:212-6 pubmed
    The role of myosin phosphorylation by myosin light chain kinase (MLCK) in regulating the invasiveness of metastatic cancer cells was investigated using the Dunning rat prostatic adenocarcinoma cell line, Mat Ly Lu, and in vitro invasion ..
  40. Godin C, Ferguson S. The angiotensin II type 1 receptor induces membrane blebbing by coupling to Rho A, Rho kinase, and myosin light chain kinase. Mol Pharmacol. 2010;77:903-11 pubmed publisher
    ..The inhibition of the downstream RhoA effectors Rho kinase (ROCK) and myosin light chain kinase (MLCK) effectively attenuated AT(1)R-mediated membrane blebbing...
  41. Niggli V, Schmid M, Nievergelt A. Differential roles of Rho-kinase and myosin light chain kinase in regulating shape, adhesion, and migration of HT1080 fibrosarcoma cells. Biochem Biophys Res Commun. 2006;343:602-8 pubmed
    We present evidence for differential roles of Rho-kinase and myosin light chain kinase (MLCK) in regulating shape, adhesion, migration, and chemotaxis of human fibrosarcoma HT1080 cells on laminin-coated surfaces...
  42. Gu L, Hu W, Antic N, Mehta R, Turner J, de Lanerolle P. Inhibiting myosin light chain kinase retards the growth of mammary and prostate cancer cells. Eur J Cancer. 2006;42:948-57 pubmed
    We have previously shown that ML-7, which inhibits myosin light chain kinase (MLCK), induces apoptosis in transformed and non-transformed cells...
  43. Han Y, Hu W, Chernaya O, Antic N, Gu L, Gupta M, et al. Increased myosin light chain kinase expression in hypertension: Regulation by serum response factor via an insertion mutation in the promoter. Mol Biol Cell. 2006;17:4039-50 pubmed
    ..Therefore, we investigated the regulation of smooth muscle myosin light chain kinase (smMLCK) by using spontaneously hypertensive rats (SHR) as an experimental model...
  44. Gao L, Grant A, Rafaels N, Stockton Porter M, Watkins T, Gao P, et al. Polymorphisms in the myosin light chain kinase gene that confer risk of severe sepsis are associated with a lower risk of asthma. J Allergy Clin Immunol. 2007;119:1111-8 pubmed
    b>Myosin light chain kinase (MYLK) is a multifunctional protein involved in regulation of airway hyperreactivity and other activities relevant to asthma...
  45. Yin F, Hoggatt A, Zhou J, Herring B. 130-kDa smooth muscle myosin light chain kinase is transcribed from a CArG-dependent, internal promoter within the mouse mylk gene. Am J Physiol Cell Physiol. 2006;290:C1599-609 pubmed
    The 130-kDa smooth muscle myosin light chain kinase (smMLCK) is a Ca2+/CaM-regulated enzyme that plays a pivotal role in the initiation of smooth muscle contraction and regulation of cellular migration and division...
  46. Mizuno Y, Isotani E, Huang J, Ding H, Stull J, Kamm K. Myosin light chain kinase activation and calcium sensitization in smooth muscle in vivo. Am J Physiol Cell Physiol. 2008;295:C358-64 pubmed publisher
    ..CaM)-dependent phosphorylation of myosin regulatory light chain (RLC) in smooth muscle by myosin light chain kinase (MLCK) and dephosphorylation by myosin light chain phosphatase (MLCP) are subject to modulatory cascades ..
  47. Lamprecht R, Margulies D, Farb C, Hou M, Johnson L, Ledoux J. Myosin light chain kinase regulates synaptic plasticity and fear learning in the lateral amygdala. Neuroscience. 2006;139:821-9 pubmed
    ..We therefore examined whether the cytoskeletal-regulatory protein, myosin light chain kinase, might contribute to fear learning in the rat lateral amygdala...
  48. Zhang W, Peng Y, Zhang G, He W, Qiao Y, Dong Y, et al. Myosin light chain kinase is necessary for tonic airway smooth muscle contraction. J Biol Chem. 2010;285:5522-31 pubmed publisher
    Different interacting signaling modules involving Ca(2+)/calmodulin-dependent myosin light chain kinase, Ca(2+)-independent regulatory light chain phosphorylation, myosin phosphatase inhibition, and actin filament-based proteins are ..
  49. Nousiainen M, Derrick P, Lafitte D, Vainiotalo P. Relative affinity constants by electrospray ionization and Fourier transform ion cyclotron resonance mass spectrometry: calmodulin binding to peptide analogs of myosin light chain kinase. Biophys J. 2003;85:491-500 pubmed
    ..used to analyze the interactions between calmodulin (CaM) and the CaM-binding sequence of smooth-muscle myosin light chain kinase both in the presence and the absence of Ca(2+)...
  50. Flores C, Ma S, Maresso K, Ober C, Garcia J. A variant of the myosin light chain kinase gene is associated with severe asthma in African Americans. Genet Epidemiol. 2007;31:296-305 pubmed
    ..In addition to a multitude of factors contributing to its pathobiology, increased amounts of myosin light chain kinase (MLCK), the central regulator of cellular contraction, have been found in airway smooth muscle from ..
  51. Zhang W, Peng Y, He W, Lv N, Chen C, Zhi G, et al. Identification and functional characterization of an aggregation domain in long myosin light chain kinase. FEBS J. 2008;275:2489-500 pubmed publisher
    The functions of long smooth muscle myosin light chain kinase (L-MLCK), a molecule with multiple domains, are poorly understood...
  52. Oury C, Sticker E, Cornelissen H, De Vos R, Vermylen J, Hoylaerts M. ATP augments von Willebrand factor-dependent shear-induced platelet aggregation through Ca2+-calmodulin and myosin light chain kinase activation. J Biol Chem. 2004;279:26266-73 pubmed
    ..We conclude that the P2X(1) ion channel induces MLC-mediated cytoskeletal rearrangements, thus contributing to SIPA and degranulation during VWF-triggered platelet activation. ..
  53. Wu C, Lu Y, Wu L, Yu L. Role of myosin light chain kinase in intestinal epithelial barrier defects in a rat model of bowel obstruction. BMC Gastroenterol. 2010;10:39 pubmed publisher
    ..b>Myosin light chain kinase (MLCK) is actively involved in the contraction of epithelial perijunctional actinomyosin ring and ..