phosphoglycerate kinase

Summary

Summary: An enzyme catalyzing the transfer of a phosphate group from 3-phospho-D-glycerate in the presence of ATP to yield 3-phospho-D-glyceroyl phosphate and ADP. EC 2.7.2.3.

Top Publications

  1. Trant C, Lacerda T, Carvalho N, Azevedo V, Rosinha G, Salcedo S, et al. The Brucella abortus phosphoglycerate kinase mutant is highly attenuated and induces protection superior to that of vaccine strain 19 in immunocompromised and immunocompetent mice. Infect Immun. 2010;78:2283-91 pubmed publisher
    ..Herein, we identified the gene encoding the phosphoglycerate kinase (PGK) of B. abortus strain 2308...
  2. Yoshioka H, Geyer C, Hornecker J, Patel K, McCarrey J. In vivo analysis of developmentally and evolutionarily dynamic protein-DNA interactions regulating transcription of the Pgk2 gene during mammalian spermatogenesis. Mol Cell Biol. 2007;27:7871-85 pubmed
    ..of the testis-specific Pgk2 gene is selectively activated in primary spermatocytes to provide a source of phosphoglycerate kinase that is critical to normal motility and fertility of mammalian spermatozoa...
  3. Xu M, McCarrey J, Hecht N. A cytoplasmic variant of the KH-type splicing regulatory protein serves as a decay-promoting factor for phosphoglycerate kinase 2 mRNA in murine male germ cells. Nucleic Acids Res. 2008;36:7157-67 pubmed publisher
    b>Phosphoglycerate kinase 2 (PGK2) is a germ cell-specific protein whose mRNA is translationally regulated in the mammalian testis...
  4. Wang E, Dai S, Qi F, Hong Tao X, Wei Q. Gene expression and clonality analysis of the androgen receptor and phosphoglycerate kinase genes in polygonal cells and cuboidal cells in so-called pulmonary sclerosing hemangioma. Mod Pathol. 2007;20:1208-15 pubmed
    ..with the methylation-sensitive enzymes HhaI or HpaII, followed by nested PCR of the androgen receptor and phosphoglycerate kinase genes. Samples with polymorphisms were identified and a clonality analysis was performed...
  5. Gondeau C, Chaloin L, Lallemand P, Roy B, Perigaud C, Barman T, et al. Molecular basis for the lack of enantioselectivity of human 3-phosphoglycerate kinase. Nucleic Acids Res. 2008;36:3620-9 pubmed publisher
    ..to reveal the molecular basis for the low enantioselectivity and the broad specificity of human 3-phosphoglycerate kinase (hPGK), an enzyme responsible for the last step of phosphorylation of many nucleotide derivatives...
  6. Krishnan P, Liou J, Cheng Y. Phosphorylation of pyrimidine L-deoxynucleoside analog diphosphates. Kinetics of phosphorylation and dephosphorylation of nucleoside analog diphosphates and triphosphates by 3-phosphoglycerate kinase. J Biol Chem. 2002;277:31593-600 pubmed
    ..We recently reported that in the last step, L-deoxynucleoside analog diphosphates are phosphorylated by 3-phosphoglycerate kinase (PGK)...
  7. Huang S, Sirikhachornkit A, Faris J, Su X, Gill B, Haselkorn R, et al. Phylogenetic analysis of the acetyl-CoA carboxylase and 3-phosphoglycerate kinase loci in wheat and other grasses. Plant Mol Biol. 2002;48:805-20 pubmed
    ..sequences of nuclear genes encoding multi-domain plastid acetyl-CoA carboxylase (ACCase) and plastid 3-phosphoglycerate kinase (PGK) to study grass evolution...
  8. Aasly J, van Diggelen O, Boer A, Brønstad G. Phosphoglycerate kinase deficiency in two brothers with McArdle-like clinical symptoms. Eur J Neurol. 2000;7:111-3 pubmed
    b>Phosphoglycerate kinase (PGK) catalyses the transfer of the acylphosphate group of 1,3-diphosphoglycerate to ADP with formation of 3-phosphoglycerate and ATP in the terminal stage of the glycolytic pathway...
  9. Szilágyi A, Ghosh M, Garman E, Vas M. A 1.8 A resolution structure of pig muscle 3-phosphoglycerate kinase with bound MgADP and 3-phosphoglycerate in open conformation: new insight into the role of the nucleotide in domain closure. J Mol Biol. 2001;306:499-511 pubmed
    3-phosphoglycerate kinase (PGK) is a typical kinase with two structural domains. The domains each bind one of the two substrates, 3-phosphoglycerate (3-PG) and MgATP...

More Information

Publications86

  1. Hwang T, Liang Y, Chien K, Yu J. Overexpression and elevated serum levels of phosphoglycerate kinase 1 in pancreatic ductal adenocarcinoma. Proteomics. 2006;6:2259-72 pubmed
    ..One of them was identified as phosphoglycerate kinase (PGK) 1, a secretable glycolytic enzyme known to participate in angiogenesis...
  2. Liu J, Jones K, Sumer H, Verma P. Stable transgene expression in human embryonic stem cells after simple chemical transfection. Mol Reprod Dev. 2009;76:580-6 pubmed publisher
    ..We demonstrated that cytomegalovirus (CMV), phosphoglycerate kinase (PGK) and human elongation factor-1alpha (EF1alpha) promoters all resulted in robust activity of a ..
  3. Morimoto A, Ueda I, Hirashima Y, Sawai Y, Usuku T, Kano G, et al. A novel missense mutation (1060G --> C) in the phosphoglycerate kinase gene in a Japanese boy with chronic haemolytic anaemia, developmental delay and rhabdomyolysis. Br J Haematol. 2003;122:1009-13 pubmed
    We report the case of a 3-year-old Japanese boy with phosphoglycerate kinase 1 (PGK1) deficiency (Online Mendelian Inheritance in Man entry 311800)...
  4. Chen K, Beck J, Huang L, Knorr C, Brenig B. Assignment of the phosphoglycerate kinase 2 (PGK2) gene to porcine chromosome 7q14-q15 by fluorescence in situ hybridization and by analysis of somatic cell and radiation hybrid panels. Anim Genet. 2004;35:71-2 pubmed
  5. Lane M, Gardner D. Inhibiting 3-phosphoglycerate kinase by EDTA stimulates the development of the cleavage stage mouse embryo. Mol Reprod Dev. 2001;60:233-40 pubmed
    ..EDTA was shown to inhibit the activity of the glycolytic enzyme, 3-phosphoglycerate kinase. Additionally, the effect of EDTA on maintaining high rates of embryo development in culture could be ..
  6. Ikemoto A, Bole D, Ueda T. Glycolysis and glutamate accumulation into synaptic vesicles. Role of glyceraldehyde phosphate dehydrogenase and 3-phosphoglycerate kinase. J Biol Chem. 2003;278:5929-40 pubmed
    ..We demonstrate that the glycolytic enzymes glyceraldehyde phosphate dehydrogenase (GAPDH) and 3-phosphoglycerate kinase (3-PGK) are enriched in synaptic vesicles, forming a functional complex, and that synaptic vesicles are ..
  7. Labroussaa F, Arricau Bouvery N, Dubrana M, Saillard C. Entry of Spiroplasma citri into Circulifer haematoceps cells involves interaction between spiroplasma phosphoglycerate kinase and leafhopper actin. Appl Environ Microbiol. 2010;76:1879-86 pubmed publisher
    ..actin-binding protein of 44 kDa was isolated by affinity chromatography and identified by LC-MS/MS as phosphoglycerate kinase (PGK)...
  8. Ijeoma O, Hollowell H, Bodnar M, Britt B. Thermodynamic analysis of the nondenaturational conformational change of baker's yeast phosphoglycerate kinase at 24 degrees C. Arch Biochem Biophys. 2008;478:206-11 pubmed publisher
    A plot of the Gibbs free energy of unfolding vs. temperature is calculated for baker's yeast phosphoglycerate kinase in 150 mM sodium phosphate (pH=7...
  9. Kiem H, Allen J, Trobridge G, Olson E, Keyser K, Peterson L, et al. Foamy-virus-mediated gene transfer to canine repopulating cells. Blood. 2007;109:65-70 pubmed
    ..These data suggest that FV vectors should be useful for stem-cell gene therapy, particularly for applications in which short transduction protocols are critical. ..
  10. Quijada L, Guerra Giraldez C, Drozdz M, Hartmann C, Irmer H, Ben Dov C, et al. Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements. Nucleic Acids Res. 2002;30:4414-24 pubmed
    ..We show here that the mRNA for the procyclic-specific cytosolic phosphoglycerate kinase PGKB, like that for EP proteins, contains a regulatory AU-rich element (ARE) that destabilises the mRNA ..
  11. Hurth C, Tassius C, Talbot J, Maali A, Moskalenko C, Minard P, et al. Enzymatic activity of immobilized yeast phosphoglycerate kinase. Biosens Bioelectron. 2007;22:2449-55 pubmed
    This work reports the first evidence that recombinant yeast phosphoglycerate kinase (PGK) is still significantly active when immobilized on glass and muscovite mica...
  12. Cheng S, Huang Y, Chen L, Hsu Y, Tsai C. Chloroplast phosphoglycerate kinase is involved in the targeting of Bamboo mosaic virus to chloroplasts in Nicotiana benthamiana plants. Plant Physiol. 2013;163:1598-608 pubmed publisher
    ..Previously, we identified that the chloroplast phosphoglycerate kinase (chl-PGK) from Nicotiana benthamiana is one of the viral RNA binding proteins involved in the BaMV ..
  13. Huang S, Sirikhachornkit A, Su X, Faris J, Gill B, Haselkorn R, et al. Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the Triticum/Aegilops complex and the evolutionary history of polyploid wheat. Proc Natl Acad Sci U S A. 2002;99:8133-8 pubmed
    ..We analyzed Acc-1 (plastid acetyl-CoA carboxylase) and Pgk-1 (plastid 3-phosphoglycerate kinase) genes to determine phylogenetic relationships among Triticum and Aegilops species of the wheat lineage ..
  14. Bentahir M, Feller G, Aittaleb M, Lamotte Brasseur J, Himri T, Chessa J, et al. Structural, kinetic, and calorimetric characterization of the cold-active phosphoglycerate kinase from the antarctic Pseudomonas sp. TACII18. J Biol Chem. 2000;275:11147-53 pubmed
    The gene encoding the phosphoglycerate kinase (PGK) from the Antarctic Pseudomonas sp. TACII18 has been cloned and found to be inserted between the genes encoding for glyceraldhyde-3-phosphate dehydrogenase and fructose aldolase...
  15. Shirakawa K, Takahashi Y, Miyajima H. Intronic mutation in the PGK1 gene may cause recurrent myoglobinuria by aberrant splicing. Neurology. 2006;66:925-7 pubmed
    A 33-year-old man with mental retardation and recurrent myoglobinuria demonstrated a deficiency in the phosphoglycerate kinase 1 (PGK1) activity of his muscles and erythrocytes...
  16. Stewart M, Haile S, Jha B, Cristodero M, Li C, Clayton C. Processing of a phosphoglycerate kinase reporter mRNA in Trypanosoma brucei is not coupled to transcription by RNA polymerase II. Mol Biochem Parasitol. 2010;172:99-106 pubmed publisher
    ..There was therefore no evidence for recruitment of the 3'-splicing apparatus by the RNA polymerase. Abundant transcripts transcribed from the rRNA locus by T7 RNA polymerase were somewhat less efficiently processed. ..
  17. Marston J, Cliff M, Reed M, Blackburn G, Hounslow A, Craven C, et al. Structural tightening and interdomain communication in the catalytic cycle of phosphoglycerate kinase. J Mol Biol. 2010;396:345-60 pubmed publisher
    Changes in amide-NH chemical shift and hydrogen exchange rates as phosphoglycerate kinase progresses through its catalytic cycle have been measured to assess whether they correlate with changes in hydrogen bonding within the protein...
  18. Colasante C, Robles A, Li C, Schwede A, Benz C, Voncken F, et al. Regulated expression of glycosomal phosphoglycerate kinase in Trypanosoma brucei. Mol Biochem Parasitol. 2007;151:193-204 pubmed
    In Trypanosoma brucei, the PGKB and PGKC genes-encoding phosphoglycerate kinase are co-transcribed as part of a polycistronic RNA...
  19. Buchachenko A, Kouznetsov D, Orlova M, Markarian A. Magnetic isotope effect of magnesium in phosphoglycerate kinase phosphorylation. Proc Natl Acad Sci U S A. 2005;102:10793-6 pubmed
    b>Phosphoglycerate kinase (PGK) is found to be controlled by a (25)Mg(2+)-related magnetic isotope effect...
  20. Mazzoni C, Torella M, Petrera A, Palermo V, Falcone C. PGK1, the gene encoding the glycolitic enzyme phosphoglycerate kinase, acts as a multicopy suppressor of apoptotic phenotypes in S. cerevisiae. Yeast. 2009;26:31-7 pubmed publisher
    ..by overexpressing yeast genes allowed the isolation of PGK1, the gene encoding the glycolytic enzyme phosphoglycerate kinase. This gene restored normal ageing, oxygen peroxide resistance and nuclear integrity in the mutant...
  21. Kovári Z, Vas M. Protein conformer selection by sequence-dependent packing contacts in crystals of 3-phosphoglycerate kinase. Proteins. 2004;55:198-209 pubmed
    In several crystal structures of 3-phosphoglycerate kinase (PGK), the two domains occupy different relative positions...
  22. Valentin C, Birgens H, Craescu C, Brødum Nielsen K, Cohen Solal M. A phosphoglycerate kinase mutant (PGK Herlev; D285V) in a Danish patient with isolated chronic hemolytic anemia: mechanism of mutation and structure-function relationships. Hum Mutat. 1998;12:280-7 pubmed
    b>Phosphoglycerate kinase (PGK) is a X-linked enzyme that plays a key role in the glycolytic pathway. Twelve different variants have already been reported...
  23. Duan Z, Lamendola D, Yusuf R, Penson R, Preffer F, Seiden M. Overexpression of human phosphoglycerate kinase 1 (PGK1) induces a multidrug resistance phenotype. Anticancer Res. 2002;22:1933-41 pubmed
    ..DNA sequencing of clone C16 demonstrates complete homology to human phosphoglycerate kinase 1 (PGK1)...
  24. Shichijo S, Azuma K, Komatsu N, Ito M, Maeda Y, Ishihara Y, et al. Two proliferation-related proteins, TYMS and PGK1, could be new cytotoxic T lymphocyte-directed tumor-associated antigens of HLA-A2+ colon cancer. Clin Cancer Res. 2004;10:5828-36 pubmed
    ..5'-aminoimidazole-4-carboxamide-1-beta-d-ribonucleotide transfolmylase/inosinicase (AICRT/I); and phosphoglycerate kinase 1 (PKG1), which was secreted by tumor cells and involved in the angiogenic process...
  25. Gondeau C, Chaloin L, Varga A, Roy B, Lallemand P, Perigaud C, et al. Differences in the transient kinetics of the binding of D-ADP and its mirror image L-ADP to human 3-phosphoglycerate kinase revealed by the presence of 3-phosphoglycerate. Biochemistry. 2008;47:3462-73 pubmed publisher
    ..The last step of the cascade may be catalyzed by 3-phosphoglycerate kinase (PGK), an enzyme that has low specificity for nucleoside diphosphate (NDP): NDP + 1,3-bisphosphoglycerate ..
  26. Flachner B, Kovári Z, Varga A, Gugolya Z, Vonderviszt F, Náray Szabó G, et al. Role of phosphate chain mobility of MgATP in completing the 3-phosphoglycerate kinase catalytic site: binding, kinetic, and crystallographic studies with ATP and MgATP. Biochemistry. 2004;43:3436-49 pubmed
    The complexes of pig muscle 3-phosphoglycerate kinase with the substrate MgATP and with the nonsubstrate Mg(2+)-free ATP have been characterized by binding, kinetic, and crystallographic studies...
  27. Haanstra J, Stewart M, Luu V, van Tuijl A, Westerhoff H, Clayton C, et al. Control and regulation of gene expression: quantitative analysis of the expression of phosphoglycerate kinase in bloodstream form Trypanosoma brucei. J Biol Chem. 2008;283:2495-507 pubmed
    Isoenzymes of phosphoglycerate kinase in Trypanosoma brucei are differentially expressed in its two main life stages...
  28. Sotiriou E, Greene P, Krishna S, Hirano M, DiMauro S. Myopathy and parkinsonism in phosphoglycerate kinase deficiency. Muscle Nerve. 2010;41:707-10 pubmed publisher
    ..b>Phosphoglycerate kinase (PGK) activity was markedly decreased in muscle, and molecular analysis of the PGK1 gene identified the p...
  29. Daly E, Wind T, Jiang X, Sun L, Hogg P. Secretion of phosphoglycerate kinase from tumour cells is controlled by oxygen-sensing hydroxylases. Biochim Biophys Acta. 2004;1691:17-22 pubmed
    Solid tumour cells employ glycolytic enzymes including phosphoglycerate kinase (PGK) to make ATP when their supply of oxygen is limiting...
  30. Bardey V, Vallet C, Robas N, Charpentier B, Thouvenot B, Mougin A, et al. Characterization of the molecular mechanisms involved in the differential production of erythrose-4-phosphate dehydrogenase, 3-phosphoglycerate kinase and class II fructose-1,6-bisphosphate aldolase in Escherichia coli. Mol Microbiol. 2005;57:1265-87 pubmed
    A gapA-pgk gene tandem coding the glyceraldehyde 3-phosphate dehydrogenase and 3-phosphoglycerate kinase, is most frequently found in bacteria...
  31. Osváth S, Köhler G, Zavodszky P, Fidy J. Asymmetric effect of domain interactions on the kinetics of folding in yeast phosphoglycerate kinase. Protein Sci. 2005;14:1609-16 pubmed
    ..A model protein system consisting of several single-tryptophan variants of the two-domain yeast phosphoglycerate kinase (PGK) and its individual domains was studied...
  32. Zerrad L, Merli A, Schröder G, Varga A, Gráczer E, Pernot P, et al. A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinase. J Biol Chem. 2011;286:14040-8 pubmed publisher
    b>Phosphoglycerate kinase (PGK) is the enzyme responsible for the first ATP-generating step of glycolysis and has been implicated extensively in oncogenesis and its development...
  33. Oslancová A, Janecek S. Evolutionary relatedness between glycolytic enzymes most frequently occurring in genomes. Folia Microbiol (Praha). 2004;49:247-58 pubmed
    ..Triosephosphate isomerase (TIM), glyceraldehyde-3-phosphate dehydrogenase (GAPD), phosphoglycerate kinase (PGK) and enolase (ENOL) were found to be present in 90 investigated genomes all together...
  34. Osváth S, Sabelko J, Gruebele M. Tuning the heterogeneous early folding dynamics of phosphoglycerate kinase. J Mol Biol. 2003;333:187-99 pubmed
    ..during the formation of a collapsed, long-lived intermediate state of the large two-domain enzyme phosphoglycerate kinase (PGK)...
  35. Hong S, Seong K, Sohn W, Song K. Molecular cloning and immunological characterization of phosphoglycerate kinase from Clonorchis sinensis. Mol Biochem Parasitol. 2000;108:207-16 pubmed
    ..b>Phosphoglycerate kinase (PGK), a glycolytic enzyme, found in many parasites, has been identified as one of the candidate ..
  36. Mukherjee K, Ghosh S, Ray M, Ray S. Purification and characterization of 3-phosphoglycerate kinase from Ehrlich ascites carcinoma cells. Indian J Biochem Biophys. 2002;39:332-41 pubmed
    3-Phosphoglycerate kinase (3-PGK) has been purified to apparent homogeneity from Ehrlich ascites carcinoma (EAC) cells by (NH4)2SO4 precipitation, gel filtration and ion-exchange chromatography...
  37. Cliff M, Bowler M, Varga A, Marston J, Szabo J, Hounslow A, et al. Transition state analogue structures of human phosphoglycerate kinase establish the importance of charge balance in catalysis. J Am Chem Soc. 2010;132:6507-16 pubmed publisher
    Transition state analogue (TSA) complexes formed by phosphoglycerate kinase (PGK) have been used to test the hypothesis that balancing of charge within the transition state dominates enzyme-catalyzed phosphoryl transfer...
  38. Estevez A. The RNA-binding protein TbDRBD3 regulates the stability of a specific subset of mRNAs in trypanosomes. Nucleic Acids Res. 2008;36:4573-86 pubmed publisher
    ..These observations raise the possibility that TbDRBD3-mRNA complexes act as a post-transcriptional operon, and provide a framework to interpret how trypanosomes regulate gene expression in the absence of transcriptional control. ..
  39. Svaasand E, Aasly J, Landsem V, Klungland H. Altered expression of PGK1 in a family with phosphoglycerate kinase deficiency. Muscle Nerve. 2007;36:679-84 pubmed
    The X-linked recessive disease phosphoglycerate kinase (PGK) deficiency is caused by altered expression of the PGK1 enzyme, which causes muscle stiffness, hemolytic anemia, and mental retardation...
  40. de Almeida J, de Moraes L, Torres F. Molecular characterization of the 3-phosphoglycerate kinase gene (PGK1) from the methylotrophic yeast Pichia pastoris. Yeast. 2005;22:725-37 pubmed
    We report the cloning of the 3-phosphoglycerate kinase gene (PGK1) from the methylotrophic yeast Pichia pastoris by a PCR approach...
  41. Kovári Z, Flachner B, Náray Szabó G, Vas M. Crystallographic and thiol-reactivity studies on the complex of pig muscle phosphoglycerate kinase with ATP analogues: correlation between nucleotide binding mode and helix flexibility. Biochemistry. 2002;41:8796-806 pubmed
    Crystal structure of the ternary complex of pig muscle phosphoglycerate kinase (PGK) with the substrate 3-phosphoglycerate (3-PG) and the Mg(2+) complex of beta,gamma-methylene-adenosine-5'-triphosphate (AMP-PCP), a nonreactive analogue ..
  42. Fan X, Sha L, Zeng J, Kang H, Zhang H, Wang X, et al. Evolutionary dynamics of the Pgk1 gene in the polyploid genus Kengyilia (Triticeae: Poaceae) and its diploid relatives. PLoS ONE. 2012;7:e31122 pubmed publisher
  43. Xu M, Hecht N. Polypyrimidine tract binding protein 2 stabilizes phosphoglycerate kinase 2 mRNA in murine male germ cells by binding to its 3'UTR. Biol Reprod. 2007;76:1025-33 pubmed
    The mRNA that encodes the testis-specific protein phosphoglycerate kinase (PGK2) is a long-lived mRNA that is transcribed in meiotic and postmeiotic male germ cells...
  44. Tsukamoto Y, Fukushima Y, Hara S, Hisabori T. Redox control of the activity of phosphoglycerate kinase in Synechocystis sp. PCC6803. Plant Cell Physiol. 2013;54:484-91 pubmed publisher
    b>Phosphoglycerate kinase catalyzes the phosphorylation of 3-phosphoglycerate to 1,3-diphosphoglycerate using ATP within the Calvin-Benson cycle. Recent proteomic analysis of thioredoxin targets in Synechocystis sp...
  45. Yan H, Yang K, Xiao H, Zou Y, Zhang W, Liu H. Over-expression of cofilin-1 and phosphoglycerate kinase 1 in astrocytomas involved in pathogenesis of radioresistance. CNS Neurosci Ther. 2012;18:729-36 pubmed publisher
    ..A total of 36 proteins were expressed differently between the 2 groups, represented by cofilin-1 and phosphoglycerate kinase 1 (PGK1), which up-regulated significantly in radioresistant astrocytomas though there was no obvious ..
  46. Ho M, Tang S, Ng W, Yang W, Leu S, Lin Y, et al. Nucleotide-binding domain of phosphoglycerate kinase 1 reduces tumor growth by suppressing COX-2 expression. Cancer Sci. 2010;101:2411-6 pubmed publisher
    b>Phosphoglycerate kinase 1 (PGK-1) is a multifunctional protein that is involved in the glycolytic pathway and the generation of the angiogenesis inhibitor angiostatin...
  47. Lallemand P, Chaloin L, Roy B, Barman T, Bowler M, Lionne C. Interaction of human 3-phosphoglycerate kinase with its two substrates: is substrate antagonism a kinetic advantage?. J Mol Biol. 2011;409:742-57 pubmed publisher
    ..3-Phosphoglycerate kinase (PGK) catalyzes phosphotransfer from 1,3-bisphosphoglycerate (bPG) to ADP to give 3-phosphoglycerate (PG) ..
  48. Bernardo García N, Bartual S, Fulde M, Bergmann S, Hermoso J. Crystallization and preliminary X-ray diffraction analysis of phosphoglycerate kinase from Streptococcus pneumoniae. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:1285-9 pubmed publisher
    b>Phosphoglycerate kinase (PGK) is a widespread two-domain enzyme that plays a critical role in the glycolytic pathway...
  49. Szabo J, Varga A, Flachner B, Konarev P, Svergun D, Zavodszky P, et al. Communication between the nucleotide site and the main molecular hinge of 3-phosphoglycerate kinase. Biochemistry. 2008;47:6735-44 pubmed publisher
    3-Phosphoglycerate kinase is a hinge-bending enzyme with substrate-assisted domain closure. However, the closure mechanism has not been described in terms of structural details...
  50. Tougard P, Bizebard T, Ritco Vonsovici M, Minard P, Desmadril M. Structure of a circularly permuted phosphoglycerate kinase. Acta Crystallogr D Biol Crystallogr. 2002;58:2018-23 pubmed
    ..The effect of the mutation on the functional properties is more easily accounted for by a restriction of hinge-bending motion than by structural changes in the protein. ..
  51. Wolf M, Müller T, Dandekar T, Pollack J. Phylogeny of Firmicutes with special reference to Mycoplasma (Mollicutes) as inferred from phosphoglycerate kinase amino acid sequence data. Int J Syst Evol Microbiol. 2004;54:871-5 pubmed
    The phylogenetic position of the Mollicutes has been re-examined by using phosphoglycerate kinase (Pgk) amino acid sequences. Hitherto unpublished sequences from Mycoplasma mycoides subsp...
  52. Flachner B, Varga A, Szabo J, Barna L, Hajdú I, Gyimesi G, et al. Substrate-assisted movement of the catalytic Lys 215 during domain closure: site-directed mutagenesis studies of human 3-phosphoglycerate kinase. Biochemistry. 2005;44:16853-65 pubmed
    3-Phosphoglycerate kinase (PGK) is a two-domain hinge-bending enzyme...
  53. Varga A, Szabo J, Flachner B, Roy B, Konarev P, Svergun D, et al. Interaction of human 3-phosphoglycerate kinase with L-ADP, the mirror image of D-ADP. Biochem Biophys Res Commun. 2008;366:994-1000 pubmed
    ..be phosphorylated to pharmacologically active triphosphate forms, the last step seems to involve human 3-phosphoglycerate kinase (hPGK)...
  54. Zhao W, Pao S, Malik F, Soh J, Fernandez S, Chirico W. A sandwich ELISA for phosphoglycerate kinase. J Immunoassay Immunochem. 2008;29:220-33 pubmed publisher
    b>Phosphoglycerate kinase (PGK1) is a key enzyme in glycolysis that can also be released from certain cells...
  55. Gloor G, Tyagi G, Abrassart D, Kingston A, Fernandes A, Dunn S, et al. Functionally compensating coevolving positions are neither homoplasic nor conserved in clades. Mol Biol Evol. 2010;27:1181-91 pubmed publisher
    We demonstrated that a pair of positions in phosphoglycerate kinase that score highly by three nonparametric covariation measures are important for function even though the positions can be occupied by aliphatic, aromatic, or charged ..
  56. Wesołowska A, Jaros S, Norbury L, Jaros D, Zygner W, Wedrychowicz H. Microarray analysis of rat immune responses to liver fluke infection following vaccination with Fasciola hepatica phosphoglycerate kinase. Exp Parasitol. 2013;134:33-8 pubmed publisher
    ..hepatica following immunization with F. hepatica phosphoglycerate kinase - a novel vaccine antigen...
  57. Concepcion J, Adjé C, Quinones W, Chevalier N, Dubourdieu M, Michels P. The expression and intracellular distribution of phosphoglycerate kinase isoenzymes in Trypanosoma cruzi. Mol Biochem Parasitol. 2001;118:111-21 pubmed
    In this paper, we report the subcellular distribution of phosphoglycerate kinase (PGK) in epimastigotes of Trypanosoma cruzi. Approximately 80% of the PGK activity was found in the cytosol, 20% in the glycosomes...
  58. Krishnan P, Gullen E, Lam W, Dutschman G, Grill S, Cheng Y. Novel role of 3-phosphoglycerate kinase, a glycolytic enzyme, in the activation of L-nucleoside analogs, a new class of anticancer and antiviral agents. J Biol Chem. 2003;278:36726-32 pubmed
    ..We studied the role of 3-phosphoglycerate kinase, a glycolytic enzyme, in the metabolism of l-nucleoside analogs, using small interfering RNAs to down-..
  59. Wang P, Saraswati S, Guan Z, Watkins C, Wurtman R, Littleton J. A Drosophila temperature-sensitive seizure mutant in phosphoglycerate kinase disrupts ATP generation and alters synaptic function. J Neurosci. 2004;24:4518-29 pubmed
    ..The nubian mutant disrupts phosphoglycerate kinase (PGK), an enzyme required for ATP generation in the terminal stage of the glycolytic pathway...
  60. Calcedo R, Ramirez Garcia A, Abad A, Rementeria A, Ponton J, Hernando F. Phosphoglycerate kinase and fructose bisphosphate aldolase of Candida albicans as new antigens recognized by human salivary IgA. Rev Iberoam Micol. 2012;29:172-4 pubmed publisher
    ..36 kDa) were found to be regulated by heat shock and by nutritional stress and they were identified as phosphoglycerate kinase and fructose bisphosphate aldolase, respectively. These glycolytic enzymes are major antigens of C...
  61. Gallois Montbrun S, Faraj A, Seclaman E, Sommadossi J, Deville Bonne D, Veron M. Broad specificity of human phosphoglycerate kinase for antiviral nucleoside analogs. Biochem Pharmacol. 2004;68:1749-56 pubmed
    ..Human phosphoglycerate kinase (hPGK) was recently reported to participate in the last step of phosphorylation of cytidine L-nucleotide ..
  62. Dhar A, Samiotakis A, Ebbinghaus S, Nienhaus L, Homouz D, Gruebele M, et al. Structure, function, and folding of phosphoglycerate kinase are strongly perturbed by macromolecular crowding. Proc Natl Acad Sci U S A. 2010;107:17586-91 pubmed publisher
    ..show how macromolecular crowding dramatically affects the structure, function, and folding landscape of phosphoglycerate kinase (PGK)...
  63. Zieker D, Königsrainer I, Tritschler I, Loffler M, Beckert S, Traub F, et al. Phosphoglycerate kinase 1 a promoting enzyme for peritoneal dissemination in gastric cancer. Int J Cancer. 2010;126:1513-20 pubmed publisher
    ..Previously, we showed elevated mRNA levels of phosphoglycerate kinase 1 (PGK1), an adenosine triphosphate-generating enzyme in the glycolytic pathway, the chemokine receptor 4 ..
  64. Lee J, Im Y, Bae J, Kim D, Kim M, Kang G, et al. Crystal structure of Thermus caldophilus phosphoglycerate kinase in the open conformation. Biochem Biophys Res Commun. 2006;350:1044-9 pubmed publisher
    b>Phosphoglycerate kinase (PGK) is a key glycolytic enzyme that catalyzes the reversible transfer of a phosphate from 1,3-bisphosphoglycerate to ADP to form 3-phosphoglycerate and ATP in the presence of magnesium...
  65. Danshina P, Geyer C, Dai Q, Goulding E, Willis W, Kitto G, et al. Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice. Biol Reprod. 2010;82:136-45 pubmed publisher
    b>Phosphoglycerate kinase 2 (PGK2), an isozyme that catalyzes the first ATP-generating step in the glycolytic pathway, is encoded by an autosomal retrogene that is expressed only during spermatogenesis...
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    ..In this review we summarize comprehensive studies with a typical hinge-bending two-domain enzyme, 3-phosphoglycerate kinase. A possible mechanism is proposed by which the two substrates that bind to different domains trigger the ..
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    b>Phosphoglycerate kinase (PGK) deficiency is a rare X-linked disease that is characterised by mild to severe haemolytic anaemia, rhabdomyolysis, and variable defects in the central nervous system...
  68. Ramirez Bajo M, Repiso A, la Ossa P, Bañón Maneus E, de Atauri P, Climent F, et al. Enzymatic and metabolic characterization of the phosphoglycerate kinase deficiency associated with chronic hemolytic anemia caused by the PGK-Barcelona mutation. Blood Cells Mol Dis. 2011;46:206-11 pubmed publisher
    Recently, we reported a new mutation of phosphoglycerate kinase (PGK), called PGK-Barcelona, which causes chronic hemolytic anemia associated with progressive neurological impairment...
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    ..Refolding of yeast phosphoglycerate kinase was initiated from the guanidine-unfolded state by stopped-flow or manual mixing and monitored by ..
  70. Yao S, Sukonnik T, Kean T, Bharadwaj R, Pasceri P, Ellis J. Retrovirus silencing, variegation, extinction, and memory are controlled by a dynamic interplay of multiple epigenetic modifications. Mol Ther. 2004;10:27-36 pubmed
    ..A molecular mechanism of competitive H1 and MeCP2 binding may account for this epigenetic interplay, and a model for variegation is discussed. ..
  71. Boone T, Burnham C, Tyrrell G. Binding of group B streptococcal phosphoglycerate kinase to plasminogen and actin. Microb Pathog. 2011;51:255-61 pubmed publisher
    The glycolytic enzyme, phosphoglycerate kinase (PGK) of group B streptococci (GBS), has previously been identified as expressed on the GBS cell surface...
  72. Patwa T, Li C, Poisson L, Kim H, Pal M, Ghosh D, et al. The identification of phosphoglycerate kinase-1 and histone H4 autoantibodies in pancreatic cancer patient serum using a natural protein microarray. Electrophoresis. 2009;30:2215-26 pubmed publisher
    ..b>Phosphoglycerate kinase-1 and histone H4 were noted to elicit a significant differential humoral response in cancer sera compared ..
  73. Beutler E. PGK deficiency. Br J Haematol. 2007;136:3-11 pubmed
    b>Phosphoglycerate kinase (PGK) deficiency is one of the relatively uncommon causes of hereditary non-spherocytic haemolytic anaemia (HNSHA). The gene encoding the erythrocyte enzyme PGK1, is X-linked...
  74. Wang J, Ying G, Wang J, Jung Y, Lu J, Zhu J, et al. Characterization of phosphoglycerate kinase-1 expression of stromal cells derived from tumor microenvironment in prostate cancer progression. Cancer Res. 2010;70:471-80 pubmed publisher
    ..microdissection and cDNA microarray analysis of CAFs in prostate tumors revealed strong upregulation of phosphoglycerate kinase-1 (PGK1), an ATP-generating glycolytic enzyme that forms part of the glycolytic pathway and is directly ..
  75. Pey A, Mesa Torres N, Chiarelli L, Valentini G. Structural and energetic basis of protein kinetic destabilization in human phosphoglycerate kinase 1 deficiency. Biochemistry. 2013;52:1160-70 pubmed publisher
    Protein kinetic destabilization is a common feature of many human genetic diseases. Human phosphoglycerate kinase 1 (PGK1) deficiency is a rare genetic disease caused by mutations in the PGK1 protein, which often shows reduced kinetic ..
  76. Zychlinski D, Schambach A, Modlich U, Maetzig T, Meyer J, Grassman E, et al. Physiological promoters reduce the genotoxic risk of integrating gene vectors. Mol Ther. 2008;16:718-25 pubmed publisher
    ..promoters than did cellular promoters derived from human genes, elongation factor-1alpha (EF1alpha) and phosphoglycerate kinase (PGK). Self-inactivating (SIN) vector design reduced but did not abolish enhancer interactions...
  77. Damaschun G, Damaschun H, Fabian H, Gast K, Krober R, Wieske M, et al. Conversion of yeast phosphoglycerate kinase into amyloid-like structure. Proteins. 2000;39:204-11 pubmed
    Yeast phosphoglycerate kinase is a structurally well-characterized enzyme consisting of 415 amino acids without disulfide bonds...