rna polymerase iii

Summary

Summary: A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure where it transcribes DNA into RNA. It has specific requirements for cations and salt and has shown an intermediate sensitivity to alpha-amanitin in comparison to RNA polymerase I and II.

Top Publications

  1. Tan A, Manley J. TLS inhibits RNA polymerase III transcription. Mol Cell Biol. 2010;30:186-96 pubmed publisher
    ..These unexpected findings indicate that TLS regulates both RNAPs II and III and supports the possibility that cross-regulation between RNA polymerases is important in maintaining normal cell growth. ..
  2. Dieci G, Fiorino G, Castelnuovo M, Teichmann M, Pagano A. The expanding RNA polymerase III transcriptome. Trends Genet. 2007;23:614-22 pubmed
    ..The extent of the Pol III transcriptome, the complexity of its regulation and its influence on cell physiology, development and disease are emerging as new areas for future research. ..
  3. Weser S, Gruber C, Hafner H, Teichmann M, Roeder R, Seifart K, et al. Transcription factor (TF)-like nuclear regulator, the 250-kDa form of Homo sapiens TFIIIB", is an essential component of human TFIIIC1 activity. J Biol Chem. 2004;279:27022-9 pubmed
    The general human RNA polymerase III transcription factor (TF) IIIC1 has hitherto been ill defined with respect to the polypeptides required for reconstitution of its activity...
  4. Michels A, Robitaille A, Buczynski Ruchonnet D, Hodroj W, Reina J, Hall M, et al. mTORC1 directly phosphorylates and regulates human MAF1. Mol Cell Biol. 2010;30:3749-57 pubmed publisher
    ..Our results describe molecular mechanisms by which mTORC1 controls human MAF1, a key repressor of RNA polymerase III transcription, and add a new branch to the signal transduction cascade immediately downstream of TORC1.
  5. Shankar R, Grover D, Brahmachari S, Mukerji M. Evolution and distribution of RNA polymerase II regulatory sites from RNA polymerase III dependant mobile Alu elements. BMC Evol Biol. 2004;4:37 pubmed
    ..Retroposition occurs by means of RNA intermediates synthesised by a RNA polymerase III promoter residing in the A-Box and B-Box in these elements...
  6. Wu J, Okada T, Fukushima T, Tsudzuki T, Sugiura M, Yukawa Y. A novel hypoxic stress-responsive long non-coding RNA transcribed by RNA polymerase III in Arabidopsis. RNA Biol. 2012;9:302-13 pubmed publisher
    ..To identify novel ncRNAs with essential functions in flowering plants, we focused attention on RNA polymerase III (Pol III) and its transcriptional activity, because most Pol III-transcribed RNAs contribute to key ..
  7. Nguyen V, Clelland B, Hockman D, Kujat Choy S, Mewhort H, Schultz M. Replication stress checkpoint signaling controls tRNA gene transcription. Nat Struct Mol Biol. 2010;17:976-81 pubmed publisher
    ..We propose that checkpoint control of the fork-pausing activity of tRNA genes complements the repertoire of replisome-targeted mechanisms by which checkpoint proteins promote faithful DNA replication. ..
  8. Rollins J, Veras I, Cabarcas S, Willis I, Schramm L. Human Maf1 negatively regulates RNA polymerase III transcription via the TFIIB family members Brf1 and Brf2. Int J Biol Sci. 2007;3:292-302 pubmed
    b>RNA polymerase III (RNA pol III) transcribes many of the small structural RNA molecules involved in processing and translation, thereby regulating the growth rate of a cell...
  9. Valentine R, Smith G. Inhibition of the RNA polymerase III-mediated dsDNA-sensing pathway of innate immunity by vaccinia virus protein E3. J Gen Virol. 2010;91:2221-9 pubmed publisher
    ..Recently, AT-rich dsDNA was reported to be transcribed into 5'-triphosphate poly(A-U) RNA by RNA polymerase III, which then activates retinoic acid-inducible gene I (RIG-I)...

More Information

Publications61

  1. Marck C, Kachouri Lafond R, Lafontaine I, Westhof E, Dujon B, Grosjean H. The RNA polymerase III-dependent family of genes in hemiascomycetes: comparative RNomics, decoding strategies, transcription and evolutionary implications. Nucleic Acids Res. 2006;34:1816-35 pubmed
    We present the first comprehensive analysis of RNA polymerase III (Pol III) transcribed genes in ten yeast genomes...
  2. Yukawa Y, Felis M, Englert M, Stojanov M, Matousek J, Beier H, et al. Plant 7SL RNA genes belong to type 4 of RNA polymerase III- dependent genes that are composed of mixed promoters. Plant J. 2005;43:97-106 pubmed
    The genes transcribed by RNA polymerase III (pol III) display a great diversity in terms of promoter structure and are placed in four groups accordingly...
  3. Zhang Q, Zhong Q, Evans A, Levy D, Zhong S. Phosphorylation of histone H3 serine 28 modulates RNA polymerase III-dependent transcription. Oncogene. 2011;30:3943-52 pubmed publisher
    Deregulation of RNA polymerase III (Pol III) transcription enhances cellular tRNAs and 5S rRNA production, leading to an increase in translational capacity to promote cell proliferation, transformation and tumor formation...
  4. Lorenzen K, Vannini A, Cramer P, Heck A. Structural biology of RNA polymerase III: mass spectrometry elucidates subcomplex architecture. Structure. 2007;15:1237-45 pubmed
    ..These data suggest that Pol III consists of a 10 subunit Pol II-like core; the peripheral heterodimers C17/25, C53/37, and C82/34; and subunit C31, which bridges between C82/34, C17/25, and the core. ..
  5. Cieśla M, Towpik J, Graczyk D, Oficjalska Pham D, Harismendy O, Suleau A, et al. Maf1 is involved in coupling carbon metabolism to RNA polymerase III transcription. Mol Cell Biol. 2007;27:7693-702 pubmed
    b>RNA polymerase III (Pol III) produces essential components of the biosynthetic machinery, and therefore its activity is tightly coupled with cell growth and metabolism...
  6. Satoh T, Ishikawa O, Ihn H, Endo H, Kawaguchi Y, Sasaki T, et al. Clinical usefulness of anti-RNA polymerase III antibody measurement by enzyme-linked immunosorbent assay. Rheumatology (Oxford). 2009;48:1570-4 pubmed publisher
    ..The measurement of anti-RNAP III antibody by ELISA is useful in routine clinical practice, because it helps diagnose SSc and identify a disease subset with severe skin and renal involvement. ..
  7. White R. RNA polymerases I and III, growth control and cancer. Nat Rev Mol Cell Biol. 2005;6:69-78 pubmed
    ..Such events might have a significant impact on the growth potential of tumours. ..
  8. Mertens C, Roeder R. Different functional modes of p300 in activation of RNA polymerase III transcription from chromatin templates. Mol Cell Biol. 2008;28:5764-76 pubmed publisher
    Transcriptional coactivators that regulate the activity of human RNA polymerase III (Pol III) in the context of chromatin have not been reported...
  9. Luo B, Heard A, Lodish H. Small interfering RNA production by enzymatic engineering of DNA (SPEED). Proc Natl Acad Sci U S A. 2004;101:5494-9 pubmed
    ..Finally, cDNA-derived siRNA libraries can be readily generated from any cell type or species, enabling genome-wide functional screens in many biological systems. ..
  10. Kenneth N, Marshall L, White R. Recruitment of RNA polymerase III in vivo. Nucleic Acids Res. 2008;36:3757-64 pubmed publisher
    ..Promoter occupancy by pol II is unaffected, despite sharing five subunits with the pol III core. These observations provide evidence for the validity in vivo of the model for pol III recruitment that was built on biochemical data. ..
  11. Roberts D, Wilson B, Huff J, Stewart A, Cairns B. Dephosphorylation and genome-wide association of Maf1 with Pol III-transcribed genes during repression. Mol Cell. 2006;22:633-44 pubmed
    Nutrient deprivation and various stress conditions repress RNA polymerase III (Pol III) transcription in S. cerevisiae...
  12. Pascali C, Teichmann M. RNA polymerase III transcription - regulated by chromatin structure and regulator of nuclear chromatin organization. Subcell Biochem. 2013;61:261-87 pubmed publisher
    b>RNA polymerase III (Pol III) transcription is regulated by modifications of the chromatin...
  13. Zhong S, Zhang C, Johnson D. Epidermal growth factor enhances cellular TATA binding protein levels and induces RNA polymerase I- and III-dependent gene activity. Mol Cell Biol. 2004;24:5119-29 pubmed
    ..Since the cellular levels of the products of these genes, tRNAs and rRNAs, determine the translational capacity of cells, this event may be an important contributor to the transforming function of EGF. ..
  14. Goodfellow S, Graham E, Kantidakis T, Marshall L, Coppins B, Oficjalska Pham D, et al. Regulation of RNA polymerase III transcription by Maf1 in mammalian cells. J Mol Biol. 2008;378:481-91 pubmed publisher
    ..These aspects of Maf1 function have been conserved between yeast and mammals and are therefore likely to be of fundamental importance in controlling pol III transcriptional activity. ..
  15. D Ambrosio C, Schmidt C, Katou Y, Kelly G, Itoh T, Shirahige K, et al. Identification of cis-acting sites for condensin loading onto budding yeast chromosomes. Genes Dev. 2008;22:2215-27 pubmed publisher
    ..The sites map to tRNA and other genes bound by the RNA polymerase III transcription factor TFIIIC, and ribosomal protein and SNR genes...
  16. Cabart P, Lee J, Willis I. Facilitated recycling protects human RNA polymerase III from repression by Maf1 in vitro. J Biol Chem. 2008;283:36108-17 pubmed publisher
    ..This indicates that recombinant Maf1 is unable to inhibit facilitated recycling. The data suggest that additional biochemical steps may be necessary for rapid Maf1-dependent repression of RNA pol III transcription. ..
  17. Cavellán E, Asp P, Percipalle P, Farrants A. The WSTF-SNF2h chromatin remodeling complex interacts with several nuclear proteins in transcription. J Biol Chem. 2006;281:16264-71 pubmed
    ..The core proteins, WSTF, SNF2h, and nuclear myosin 1, are associated with the RNA polymerase III genes 5 S rRNA genes and 7SL, and post-transcriptional silencing of WSTF reduces the levels of these ..
  18. Werner M, Thuriaux P, Soutourina J. Structure-function analysis of RNA polymerases I and III. Curr Opin Struct Biol. 2009;19:740-5 pubmed publisher
    ..Unexpectedly, even though Pol I and III, but not Pol II, have an intrinsic RNA cleavage activity, it was found that TFIIS Pol II cleavage stimulation factor also played a general role in Pol III transcription. ..
  19. Moqtaderi Z, Wang J, Raha D, White R, Snyder M, Weng Z, et al. Genomic binding profiles of functionally distinct RNA polymerase III transcription complexes in human cells. Nat Struct Mol Biol. 2010;17:635-40 pubmed publisher
    Genome-wide occupancy profiles of five components of the RNA polymerase III (Pol III) machinery in human cells identified the expected tRNA and noncoding RNA targets and revealed many additional Pol III-associated loci, mostly near short ..
  20. Moir R, Lee J, Haeusler R, Desai N, Engelke D, Willis I. Protein kinase A regulates RNA polymerase III transcription through the nuclear localization of Maf1. Proc Natl Acad Sci U S A. 2006;103:15044-9 pubmed
    ..Finally, we report a previously undescribed phenotype for Maf1 in tRNA gene-mediated silencing of nearby RNA pol II transcription. ..
  21. Boguta M, Graczyk D. RNA polymerase III under control: repression and de-repression. Trends Biochem Sci. 2011;36:451-6 pubmed publisher
    The synthesis of tRNA by yeast RNA polymerase III (Pol III) is regulated in response to changing environmental conditions...
  22. Ferrari R, Rivetti C, Acker J, Dieci G. Distinct roles of transcription factors TFIIIB and TFIIIC in RNA polymerase III transcription reinitiation. Proc Natl Acad Sci U S A. 2004;101:13442-7 pubmed
    ..The data reveal an unexpected complexity in the Pol III reinitiation mechanism and suggest the existence of a handing-back network between Pol III, TFIIIC, and TFIIIB on actively transcribed class III genes. ..
  23. Blanc V, Adams J. Ty1 insertions in intergenic regions of the genome of Saccharomyces cerevisiae transcribed by RNA polymerase III have no detectable selective effect. FEMS Yeast Res. 2004;4:487-91 pubmed
    ..Ty1 of Saccharomyces cerevisiae inserts preferentially into intergenic regions in the vicinity of RNA polymerase III-transcribed genes...
  24. Zhong S, Machida K, Tsukamoto H, Johnson D. Alcohol induces RNA polymerase III-dependent transcription through c-Jun by co-regulating TATA-binding protein (TBP) and Brf1 expression. J Biol Chem. 2011;286:2393-401 pubmed publisher
    ..Together, these results identify a new class of genes that are regulated by alcohol through the co-regulation of TFIIIB components and define a central role for c-Jun in this process. ..
  25. Soprano A, Abe V, Smetana J, Benedetti C. Citrus MAF1, a repressor of RNA polymerase III, binds the Xanthomonas citri canker elicitor PthA4 and suppresses citrus canker development. Plant Physiol. 2013;163:232-42 pubmed publisher
    ..Here, we show that PthA4 specifically interacted with the sweet orange MAF1 (CsMAF1) protein, an RNA polymerase III (Pol III) repressor that controls ribosome biogenesis and cell growth in yeast (Saccharomyces cerevisiae) ..
  26. Diebel K, Claypool D, van Dyk L. A conserved RNA polymerase III promoter required for gammaherpesvirus TMER transcription and microRNA processing. Gene. 2014;544:8-18 pubmed publisher
    Canonical RNA polymerase III (pol III) type 2 promoters contain a single A and B box and are well documented for their role in tRNA and SINE transcription in eukaryotic cells...
  27. Kovrigina E, Yang L, Pfund E, Altman S. Regulated expression of functional external guide sequences in mammalian cells using a U6 RNA polymerase III promoter. RNA. 2005;11:1588-95 pubmed
    ..The amounts of protein of both gene products are severely reduced when the EGSs are made. Several other, but not all, of the protein subunits of RNase P are also inhibited in both mRNA and protein levels when Rpp38 mRNA is targeted. ..
  28. Amarzguioui M, Rossi J, Kim D. Approaches for chemically synthesized siRNA and vector-mediated RNAi. FEBS Lett. 2005;579:5974-81 pubmed
  29. Ducrot C, Lefebvre O, Landrieux E, Guirouilh Barbat J, Sentenac A, Acker J. Reconstitution of the yeast RNA polymerase III transcription system with all recombinant factors. J Biol Chem. 2006;281:11685-92 pubmed
    ..rTFIIIC and rTFIIIB directed a low level of basal transcription, much weaker than with the crude B'' fraction, suggesting the existence of auxiliary factors that could modulate the yeast RNA polymerase III transcription system.
  30. White R. RNA polymerases I and III, non-coding RNAs and cancer. Trends Genet. 2008;24:622-9 pubmed publisher
    ..The resultant increases in rRNA and tRNA expression might promote the generation of cancers. ..
  31. Pagano A, Castelnuovo M, Tortelli F, Ferrari R, Dieci G, Cancedda R. New small nuclear RNA gene-like transcriptional units as sources of regulatory transcripts. PLoS Genet. 2007;3:e1 pubmed
    ..By elucidating the function of one of them, we provide evidence for the existence of a sense/antisense-based gene-regulation network where part of the polymerase III transcriptome could control its polymerase II counterpart. ..
  32. Geiduschek E, Kassavetis G. Transcription: adjusting to adversity by regulating RNA polymerase. Curr Biol. 2006;16:R849-51 pubmed
    ..studies have shown that this response is signaled, in part, by multiple pathways that converge on Maf1, leading to a change of this protein's phosphorylation state and its relocation to the nucleus, where it represses RNA polymerase III.
  33. Rideout E, Marshall L, Grewal S. Drosophila RNA polymerase III repressor Maf1 controls body size and developmental timing by modulating tRNAiMet synthesis and systemic insulin signaling. Proc Natl Acad Sci U S A. 2012;109:1139-44 pubmed publisher
    ..The key effectors underlying this growth are, however, unclear. Here we show that Maf1, a repressor of RNA polymerase III-dependent tRNA transcription, is an important mediator of nutrient-dependent growth in Drosophila...
  34. Englert M, Felis M, Junker V, Beier H. Novel upstream and intragenic control elements for the RNA polymerase III-dependent transcription of human 7SL RNA genes. Biochimie. 2004;86:867-74 pubmed
    ..The Hs7SL-1 gene has recently been classified as type 4 of RNA polymerase III (pol III)-transcribed genes as it was demonstrated that mutations in an external transcriptional activator (..
  35. Fernández Tornero C, Bottcher B, Rashid U, Steuerwald U, Flörchinger B, Devos D, et al. Conformational flexibility of RNA polymerase III during transcriptional elongation. EMBO J. 2010;29:3762-72 pubmed publisher
  36. Owen T, O Neil J, Dawson C, Hu C, Chen X, Yao Y, et al. Epstein-Barr virus-encoded EBNA1 enhances RNA polymerase III-dependent EBER expression through induction of EBER-associated cellular transcription factors. Mol Cancer. 2010;9:241 pubmed publisher
    ..Virus (EBV)-encoded RNAs (EBERs) are non-polyadenylated RNA molecules transcribed from the EBV genome by RNA polymerase III (pol III)...
  37. Schoenen F, Wirth B. The zinc finger protein ZNF297B interacts with BDP1, a subunit of TFIIIB. Biol Chem. 2006;387:277-84 pubmed
    ..In the first third of the gene, the subunit of the RNA polymerase III (Pol III) transcription factor complex (TFIIIB alpha/beta) is encoded...
  38. Reina J, Azzouz T, Hernandez N. Maf1, a new player in the regulation of human RNA polymerase III transcription. PLoS ONE. 2006;1:e134 pubmed
    Human RNA polymerase III (pol III) transcription is regulated by several factors, including the tumor suppressors P53 and Rb, and the proto-oncogene c-Myc...
  39. Shor B, Wu J, Shakey Q, Toral Barza L, Shi C, Follettie M, et al. Requirement of the mTOR kinase for the regulation of Maf1 phosphorylation and control of RNA polymerase III-dependent transcription in cancer cells. J Biol Chem. 2010;285:15380-92 pubmed publisher
    ..phospho-proteomics and identified a Ser-75-containing phosphopeptide from Maf1, a known repressor of RNA polymerase III (Pol III) transcription...
  40. Vannini A. A structural perspective on RNA polymerase I and RNA polymerase III transcription machineries. Biochim Biophys Acta. 2013;1829:258-64 pubmed publisher
    ..This article is part of a Special Issue entitled: Transcription by Odd Pols. ..
  41. Meyer O, de Chaisemartin L, Nicaise Roland P, Cabane J, Tubach F, Dieude P, et al. Anti-RNA polymerase III antibody prevalence and associated clinical manifestations in a large series of French patients with systemic sclerosis: a cross-sectional study. J Rheumatol. 2010;37:125-30 pubmed publisher
    To determine the prevalence of anti-RNA polymerase III autoantibodies in French patients with systemic sclerosis (SSc) and to identify the associated clinical manifestations...
  42. Huang Y, Intine R, Mozlin A, Hasson S, Maraia R. Mutations in the RNA polymerase III subunit Rpc11p that decrease RNA 3' cleavage activity increase 3'-terminal oligo(U) length and La-dependent tRNA processing. Mol Cell Biol. 2005;25:621-36 pubmed
    Termination by RNA polymerase III (Pol III) produces RNAs whose 3' oligo(U) termini are bound by La protein, a chaperone that protects RNAs from 3' exonucleases and promotes their maturation...
  43. Dumay Odelot H, Durrieu Gaillard S, Da Silva D, Roeder R, Teichmann M. Cell growth- and differentiation-dependent regulation of RNA polymerase III transcription. Cell Cycle. 2010;9:3687-99 pubmed
    b>RNA polymerase III transcribes small untranslated RNAs that fulfill essential cellular functions in regulating transcription, RNA processing, translation and protein translocation...
  44. Moir R, Lee J, Willis I. Recovery of RNA polymerase III transcription from the glycerol-repressed state: revisiting the role of protein kinase CK2 in Maf1 phosphoregulation. J Biol Chem. 2012;287:30833-41 pubmed publisher
    ..Together, these data do not support the proposed requirement for CK2 phosphorylation of Maf1 during derepression of pol III transcription...
  45. Gilinger G, Luo H, Bellofatto V. In vivo transcription analysis utilizing chromatin immunoprecipation reveals a role for trypanosome transcription factor PBP-1 in RNA polymerase III-dependent transcription. Mol Biochem Parasitol. 2004;134:169-73 pubmed
  46. Crighton D, Woiwode A, Zhang C, Mandavia N, Morton J, Warnock L, et al. p53 represses RNA polymerase III transcription by targeting TBP and inhibiting promoter occupancy by TFIIIB. EMBO J. 2003;22:2810-20 pubmed
    ..Together our results support the idea that p53 represses RNA pol III transcription through direct interactions with TBP, preventing promoter occupancy by TFIIIB. ..
  47. Carrière L, Graziani S, Alibert O, Ghavi Helm Y, Boussouar F, Humbertclaude H, et al. Genomic binding of Pol III transcription machinery and relationship with TFIIS transcription factor distribution in mouse embryonic stem cells. Nucleic Acids Res. 2012;40:270-83 pubmed publisher
    ..We found that, as in Saccharomyces cerevisiae, TFIIS is associated with class III genes and also with SINEs suggesting that TFIIS is a Pol III transcription factor in mammals. ..
  48. Canella D, Praz V, Reina J, Cousin P, Hernandez N. Defining the RNA polymerase III transcriptome: Genome-wide localization of the RNA polymerase III transcription machinery in human cells. Genome Res. 2010;20:710-21 pubmed publisher
    Our view of the RNA polymerase III (Pol III) transcription machinery in mammalian cells arises mostly from studies of the RN5S (5S) gene, the Ad2 VAI gene, and the RNU6 (U6) gene, as paradigms for genes with type 1, 2, and 3 promoters...
  49. Faucher B, Stein P, Granel B, Weiller P, Disdier P, Serratrice J, et al. Low prevalence of anti-RNA polymerase III antibodies in a French scleroderma population: anti-RNA polymerase III scleroderma. Eur J Intern Med. 2010;21:114-7 pubmed publisher
    Anti-RNA polymerase III antibodies (anti-RNAP III) have been reported as potential immune markers of Systemic Sclerosis (SSc). Until now, their clinical use was disregarded because of technical difficulties to perform immunoprecipitation...
  50. Scott K, White C, Willard H. An RNA polymerase III-dependent heterochromatin barrier at fission yeast centromere 1. PLoS ONE. 2007;2:e1099 pubmed
    ..unlike previously characterized barriers, requires the association of both transcription factor IIIC and RNA Polymerase III. Although the cen1 tDNA(Ala) gene is actively transcribed, barrier activity is independent of ..
  51. Zhou H, Huang C, Xia X. A tightly regulated Pol III promoter for synthesis of miRNA genes in tandem. Biochim Biophys Acta. 2008;1779:773-9 pubmed publisher
    ..This newly developed U6 miRNA system would make multi-gene silencing efficient and reversible. ..
  52. Yuan C, Zhao X, Florens L, Swanson S, Washburn M, Hernandez N. CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription. Mol Cell Biol. 2007;27:8729-38 pubmed
    ..for eukaryotic transcription regulation, but little is known about chromatin-modifying activities acting on RNA polymerase III (Pol III)-transcribed genes...