rna polymerase i

Summary

Summary: A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. The enzyme functions in the nucleolar structure and transcribes DNA into RNA. It has different requirements for cations and salts than RNA polymerase II and III and is not inhibited by alpha-amanitin.

Top Publications

  1. Vannini A. A structural perspective on RNA polymerase I and RNA polymerase III transcription machineries. Biochim Biophys Acta. 2013;1829:258-64 pubmed publisher
    b>RNA polymerase I and III are responsible for the bulk of nuclear transcription in actively growing cells and their activity impacts the cellular biosynthetic capacity...
  2. Braglia P, Kawauchi J, Proudfoot N. Co-transcriptional RNA cleavage provides a failsafe termination mechanism for yeast RNA polymerase I. Nucleic Acids Res. 2011;39:1439-48 pubmed publisher
    ..An intact Reb1-binding site is also required for Rnt1-independent termination. Consequently our results reconcile the original Reb1-mediated termination pathway as part of a failsafe mechanism for this essential transcription process. ..
  3. Ogawa Y, Sugiura K, Kato K, Tohya Y, Akashi H. Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I. J Gen Virol. 2007;88:3385-90 pubmed
    ..In the present study, we describe a rescue system for Akabane virus (family Bunyaviridae) that uses cDNAs and RNA polymerase I instead of T7 RNA polymerase...
  4. Strohner R, Nemeth A, Nightingale K, Grummt I, Becker P, Längst G. Recruitment of the nucleolar remodeling complex NoRC establishes ribosomal DNA silencing in chromatin. Mol Cell Biol. 2004;24:1791-8 pubmed
    ..underlying NoRC-directed silencing of rDNA transcription, we investigated the effect of recombinant NoRC on RNA polymerase I transcription on reconstituted chromatin templates...
  5. Meraner J, Lechner M, Loidl A, Goralik Schramel M, Voit R, Grummt I, et al. Acetylation of UBF changes during the cell cycle and regulates the interaction of UBF with RNA polymerase I. Nucleic Acids Res. 2006;34:1798-806 pubmed
    The upstream binding factor UBF, an activator of RNA polymerase I transcription, is posttranslationally modified by phosphorylation and acetylation. We found that in NIH3T3 cells, UBF is acetylated in S-phase but not in G1-phase...
  6. Hannan K, Sanij E, Rothblum L, Hannan R, Pearson R. Dysregulation of RNA polymerase I transcription during disease. Biochim Biophys Acta. 2013;1829:342-60 pubmed publisher
    Transcription of the ribosomal RNA genes by the dedicated RNA polymerase I enzyme and subsequent processing of the ribosomal RNA are fundamental control steps in the synthesis of functional ribosomes...
  7. Murakami S, Horimoto T, Yamada S, Kakugawa S, Goto H, Kawaoka Y. Establishment of canine RNA polymerase I-driven reverse genetics for influenza A virus: its application for H5N1 vaccine production. J Virol. 2008;82:1605-9 pubmed
  8. Landeira D, Navarro M. Nuclear repositioning of the VSG promoter during developmental silencing in Trypanosoma brucei. J Cell Biol. 2007;176:133-9 pubmed
    ..Subsequently, the VSG promoter region was subjected to chromatin condensation. We propose a model whereby the VSG expression site pol I promoter is selectively targeted by temporal nuclear repositioning during developmental silencing. ..
  9. Schneider D, French S, Osheim Y, Bailey A, Vu L, Dodd J, et al. RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing. Proc Natl Acad Sci U S A. 2006;103:12707-12 pubmed
    ..These data suggest that Spt4p, Spt5p, and, potentially, other regulators of Pol I transcription elongation play important roles in coupling rRNA transcription to its processing and ribosome assembly. ..

More Information

Publications62

  1. Aprikian P, Moorefield B, Reeder R. New model for the yeast RNA polymerase I transcription cycle. Mol Cell Biol. 2001;21:4847-55 pubmed
    Using an immobilized template assay, we observed two steps in assembly of the yeast RNA polymerase I (Pol I) preinitiation complex: stable binding of upstream activating factor (UAF) followed by recruitment of Pol I-Rrn3p and core factor (..
  2. Conconi A, Paquette M, Fahy D, Bespalov V, Smerdon M. Repair-independent chromatin assembly onto active ribosomal genes in yeast after UV irradiation. Mol Cell Biol. 2005;25:9773-83 pubmed
    ..To date, only the passage of replication machinery was thought to package ribosomal genes in nucleosomes. In this report, we show that early events after formation of UV photoproducts in DNA also promote chromatin assembly. ..
  3. Groseth A, Feldmann H, Theriault S, Mehmetoglu G, Flick R. RNA polymerase I-driven minigenome system for Ebola viruses. J Virol. 2005;79:4425-33 pubmed publisher
    ..roles for transcription and replication in the reduced pathogenicity of Reston ebolavirus, we developed an RNA polymerase I (Pol I)-driven minigenome system...
  4. Oskarsson T, Trumpp A. The Myc trilogy: lord of RNA polymerases. Nat Cell Biol. 2005;7:215-7 pubmed
  5. Yuan X, Feng W, Imhof A, Grummt I, Zhou Y. Activation of RNA polymerase I transcription by cockayne syndrome group B protein and histone methyltransferase G9a. Mol Cell. 2007;27:585-95 pubmed
    ..Here we show that a complex consisting of CSB, RNA polymerase I (Pol I), and histone methyltransferase G9a is present at active rRNA genes...
  6. Hofmann W, Johnson T, Klapczynski M, Fan J, de Lanerolle P. From transcription to transport: emerging roles for nuclear myosin I. Biochem Cell Biol. 2006;84:418-26 pubmed
    ..Moreover, this movement is based on an active and directed process that is facilitated by an acto-NMI complex, establishing for the first time a functional role for a motor complex consisting of actin and a myosin in the nucleus. ..
  7. Iben S, Tschochner H, Bier M, Hoogstraten D, Hozak P, Egly J, et al. TFIIH plays an essential role in RNA polymerase I transcription. Cell. 2002;109:297-306 pubmed
    ..mouse, part of cellular TFIIH is localized within the nucleolus and is associated with subpopulations of both RNA polymerase I and the basal factor TIF-IB...
  8. Belin S, Kindbeiter K, Hacot S, Albaret M, Roca Martinez J, Therizols G, et al. Uncoupling ribosome biogenesis regulation from RNA polymerase I activity during herpes simplex virus type 1 infection. RNA. 2010;16:131-40 pubmed publisher
    ..At present, the most documented way to modulate ribosome biogenesis involves control of rDNA transcription by RNA polymerase I (RNA Pol I)...
  9. Toussaint M, Levasseur G, Tremblay M, Paquette M, Conconi A. Psoralen photocrosslinking, a tool to study the chromatin structure of RNA polymerase I--transcribed ribosomal genes. Biochem Cell Biol. 2005;83:449-59 pubmed
    The chromatin structure of RNA polymerase I--transcribed ribosomal DNA (rDNA) is well characterized. In most organisms, i.e., lower eukaryotes, plants, and animals, only a fraction of ribosomal genes are transcriptionally active...
  10. Granneman S, Baserga S. Crosstalk in gene expression: coupling and co-regulation of rDNA transcription, pre-ribosome assembly and pre-rRNA processing. Curr Opin Cell Biol. 2005;17:281-6 pubmed
    ..Unexpectedly, optimal rDNA transcription requires the presence of a defined subset of components of the pre-rRNA processing machinery. ..
  11. Walgraffe D, Devaux S, Lecordier L, Dierick J, Dieu M, Van den Abbeele J, et al. Characterization of subunits of the RNA polymerase I complex in Trypanosoma brucei. Mol Biochem Parasitol. 2005;139:249-60 pubmed
    The Trypanosoma brucei homologue of the RNA polymerase I (RNA Pol I) subunit Rpa12p of Saccharomyces cerevisiae was cloned and characterized...
  12. Daniels J, Gull K, Wickstead B. The trypanosomatid-specific N terminus of RPA2 is required for RNA polymerase I assembly, localization, and function. Eukaryot Cell. 2012;11:662-72 pubmed publisher
    African trypanosomes are the only organisms known to use RNA polymerase I (pol I) to transcribe protein-coding genes...
  13. Saez Vasquez J, Meissner M, Pikaard C. RNA polymerase I holoenzyme-promoter complexes include an associated CK2-like protein kinase. Plant Mol Biol. 2001;47:449-59 pubmed
    In eukaryotes, RNA polymerase I (pol I) transcribes the tandemly repeated genes that encode the precursor of 18S, 5.8S and 25S ribosomal RNAs...
  14. Grierson P, Lillard K, Behbehani G, Combs K, Bhattacharyya S, Acharya S, et al. BLM helicase facilitates RNA polymerase I-mediated ribosomal RNA transcription. Hum Mol Genet. 2012;21:1172-83 pubmed publisher
    ..lead to BS, localizes to promyelocytic leukemia protein bodies and to the nucleolus of the cell, the site of RNA polymerase I-mediated ribosomal RNA (rRNA) transcription...
  15. Naidu S, Friedrich J, Russell J, Zomerdijk J. TAF1B is a TFIIB-like component of the basal transcription machinery for RNA polymerase I. Science. 2011;333:1640-2 pubmed publisher
  16. Grandori C, Gomez Roman N, Felton Edkins Z, Ngouenet C, Galloway D, Eisenman R, et al. c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I. Nat Cell Biol. 2005;7:311-8 pubmed
    ..We propose that stimulation of rRNA synthesis by c-Myc is a key pathway driving cell growth and tumorigenesis. ..
  17. Drygin D, Lin A, Bliesath J, Ho C, O Brien S, Proffitt C, et al. Targeting RNA polymerase I with an oral small molecule CX-5461 inhibits ribosomal RNA synthesis and solid tumor growth. Cancer Res. 2011;71:1418-30 pubmed publisher
    ..Our findings position CX-5461 for investigational clinical trials as a potent, selective, and orally administered agent for cancer treatment. ..
  18. Figueiredo L, Cross G. Nucleosomes are depleted at the VSG expression site transcribed by RNA polymerase I in African trypanosomes. Eukaryot Cell. 2010;9:148-54 pubmed publisher
    In most eukaryotes, RNA polymerase I (Pol I) exclusively transcribes long arrays of identical rRNA genes (ribosomal DNA [rDNA])...
  19. Roger B, Moisand A, Amalric F, Bouvet P. Repression of RNA polymerase I transcription by nucleolin is independent of the RNA sequence that is transcribed. J Biol Chem. 2002;277:10209-19 pubmed
    ..that the interaction of nucleolin with the rDNA sequence and with nascent pre-rRNA leads to the blocking of RNA polymerase I (RNA pol I) transcription...
  20. Zhou Y, Santoro R, Grummt I. The chromatin remodeling complex NoRC targets HDAC1 to the ribosomal gene promoter and represses RNA polymerase I transcription. EMBO J. 2002;21:4632-40 pubmed
    ..The results suggest that NoRC silences the rDNA locus by targeting the SIN3 corepressor complex to the rDNA promoter, thereby establishing a repressed chromatin structure. ..
  21. Pochukalina G, Parfenov V. [Nucleolus transformation in oocytes of mouse antral follicles. Revealing of coilin and RNA polymerase I complex components]. Tsitologiia. 2008;50:671-80 pubmed
    ..Considerable changes in RNA polymerase I distribution and its colocalization with coilin at the periphery of postnucleolus were revealed...
  22. Geiger S, Lorenzen K, Schreieck A, Hanecker P, Kostrewa D, Heck A, et al. RNA polymerase I contains a TFIIF-related DNA-binding subcomplex. Mol Cell. 2010;39:583-94 pubmed publisher
    ..Thus, Pol I/III subunits that have no counterparts in Pol II are evolutionarily related to Pol II initiation factors and may have evolved to mediate promoter specificity and transcription processivity. ..
  23. Van Mullem V, Landrieux E, Vandenhaute J, Thuriaux P. Rpa12p, a conserved RNA polymerase I subunit with two functional domains. Mol Microbiol. 2002;43:1105-13 pubmed
    Rpa12p is a subunit of RNA polymerase I formed of two zinc-binding domains. The N-terminal zinc region (positions 1-60) is poorly conserved from yeast to man. The C-terminal domain contains an invariant Q.RSADE.T...
  24. Ghoshal K, Majumder S, Datta J, Motiwala T, Bai S, Sharma S, et al. Role of human ribosomal RNA (rRNA) promoter methylation and of methyl-CpG-binding protein MBD2 in the suppression of rRNA gene expression. J Biol Chem. 2004;279:6783-93 pubmed
    ..To study the effect of CpG methylation on RNA polymerase I (pol I)-transcribed rRNA genes, we constructed pHrD-IRES-Luc (human rRNA promoter-luciferase reporter)...
  25. Moss T, Stefanovsky V. At the center of eukaryotic life. Cell. 2002;109:545-8 pubmed
    ..The ribosomal RNA genes encode the enzymatic scaffold of the ribosome and thereby perform perhaps the most basic of all housekeeping functions. However, recent data suggests that they might also control important aspects of cell behavior. ..
  26. Reiner R, Krasnov Yoeli N, Dehtiar Y, Jarrous N. Function and assembly of a chromatin-associated RNase P that is required for efficient transcription by RNA polymerase I. PLoS ONE. 2008;3:e4072 pubmed publisher
  27. Laurincik J, Schmoll F, Mahabir E, Schneider H, Stojkovic M, Zakhartchenko V, et al. Nucleolar proteins and ultrastructure in bovine in vivo developed, in vitro produced, and parthenogenetic cleavage-stage embryos. Mol Reprod Dev. 2003;65:73-85 pubmed
    ..of key nucleolar proteins involved in rRNA transcription (topoisomerase I, upstream binding factor, and RNA polymerase I) and processing (fibrillarin, nucleolin, and nucleophosmin) in in vivo developed, in vitro produced, and ..
  28. Mais C, Wright J, Prieto J, Raggett S, McStay B. UBF-binding site arrays form pseudo-NORs and sequester the RNA polymerase I transcription machinery. Genes Dev. 2005;19:50-64 pubmed
    ..The architectural RNA polymerase I (pol I) transcription factor UBF binds extensively across rDNA throughout the cell cycle...
  29. Wai H, Johzuka K, Vu L, Eliason K, Kobayashi T, Horiuchi T, et al. Yeast RNA polymerase I enhancer is dispensable for transcription of the chromosomal rRNA gene and cell growth, and its apparent transcription enhancement from ectopic promoters requires Fob1 protein. Mol Cell Biol. 2001;21:5541-53 pubmed
    ..cells with disrupted nucleolar structures, suggesting that reporter genes are in general poorly accessible to RNA polymerase I (Pol I) machinery in the nucleolus and that the enhancer improves accessibility...
  30. Sanij E, Poortinga G, Sharkey K, Hung S, Holloway T, Quin J, et al. UBF levels determine the number of active ribosomal RNA genes in mammals. J Cell Biol. 2008;183:1259-74 pubmed publisher
    ..regulating the number of active copies of the approximately 200 ribosomal RNA (rRNA) genes transcribed by RNA polymerase I are unclear...
  31. Zhang Y, Sikes M, Beyer A, Schneider D. The Paf1 complex is required for efficient transcription elongation by RNA polymerase I. Proc Natl Acad Sci U S A. 2009;106:2153-8 pubmed publisher
    Regulation of RNA polymerase I (Pol I) transcription is critical for controlling ribosome synthesis. Most previous investigations into Pol I transcription regulation have focused on transcription initiation...
  32. Young D, Hassan M, Pratap J, Galindo M, Zaidi S, Lee S, et al. Mitotic occupancy and lineage-specific transcriptional control of rRNA genes by Runx2. Nature. 2007;445:442-6 pubmed
    ..Although rRNA transcriptional control by RNA polymerase I (Pol I) and associated factors is well studied, the lineage-specific mechanisms governing rRNA expression ..
  33. Meng T, Kiener T, Kwang J. RNA polymerase I-driven reverse genetics system for enterovirus 71 and its implications for vaccine production. Virol J. 2012;9:238 pubmed publisher
    ..full length genome of EV71 regardless of its genetic diversity, and the subsequent construction of a human RNA polymerase I-driven reverse genetics (RG) system to produce pure virus stocks in Vero cell within 10 days were described...
  34. Stefanovsky V, Langlois F, Gagnon Kugler T, Rothblum L, Moss T. Growth factor signaling regulates elongation of RNA polymerase I transcription in mammals via UBF phosphorylation and r-chromatin remodeling. Mol Cell. 2006;21:629-39 pubmed
    Synthesis of the 45S rRNA by RNA polymerase I limits cell growth. Knowledge of the mechanism of its regulation is therefore key to understanding growth control...
  35. Chen D, Belmont A, Huang S. Upstream binding factor association induces large-scale chromatin decondensation. Proc Natl Acad Sci U S A. 2004;101:15106-11 pubmed
    ..These studies also provide an in vivo model simulating ribosomal DNA transactivation outside the nucleolus, allowing temporal and spatial analyses of chromatin remodeling and assembly of the pol I transcription machinery. ..
  36. Chen D, Dundr M, Wang C, Leung A, Lamond A, Misteli T, et al. Condensed mitotic chromatin is accessible to transcription factors and chromatin structural proteins. J Cell Biol. 2005;168:41-54 pubmed
    ..hypothesis and to investigate the dynamics of mitotic chromatin, we evaluate the exchange kinetics of several RNA polymerase I transcription factors and nucleosome components on mitotic chromatin in living cells...
  37. El Hage A, Koper M, Kufel J, Tollervey D. Efficient termination of transcription by RNA polymerase I requires the 5' exonuclease Rat1 in yeast. Genes Dev. 2008;22:1069-81 pubmed publisher
    ..We report that the activity of Rat1 is also required for efficient termination by RNA polymerase I (Pol I) on the rDNA...
  38. Imamura S, Hanaoka M, Tanaka K. The plant-specific TFIIB-related protein, pBrp, is a general transcription factor for RNA polymerase I. EMBO J. 2008;27:2317-27 pubmed publisher
    ..CmpBrp was shown to co-immunoprecipitate and co-localize with the RNA polymerase I subunit, CmRPA190, in the cell...
  39. Laferté A, Favry E, Sentenac A, Riva M, Carles C, Chédin S. The transcriptional activity of RNA polymerase I is a key determinant for the level of all ribosome components. Genes Dev. 2006;20:2030-40 pubmed
  40. Glover L, Horn D. Repression of polymerase I-mediated gene expression at Trypanosoma brucei telomeres. EMBO Rep. 2006;7:93-9 pubmed
    ..Unusually, it uses RNA polymerase I (Pol I) for mono-telomeric expression of variant surface glycoprotein (VSG) genes in bloodstream-form cells...
  41. Yuan X, Zhao J, Zentgraf H, Hoffmann Rohrer U, Grummt I. Multiple interactions between RNA polymerase I, TIF-IA and TAF(I) subunits regulate preinitiation complex assembly at the ribosomal gene promoter. EMBO Rep. 2002;3:1082-7 pubmed
    ..is associated with a fraction of the TBP-containing factor TIF-IB/SL1 and the initiation-competent form of RNA polymerase I (Pol I)...
  42. Philimonenko V, Zhao J, Iben S, Dingová H, Kysela K, Kahle M, et al. Nuclear actin and myosin I are required for RNA polymerase I transcription. Nat Cell Biol. 2004;6:1165-72 pubmed
    ..Both proteins are associated with rDNA and are required for RNA polymerase I (Pol I) transcription...
  43. Massin P, Rodrigues P, Marasescu M, van der Werf S, Naffakh N. Cloning of the chicken RNA polymerase I promoter and use for reverse genetics of influenza A viruses in avian cells. J Virol. 2005;79:13811-6 pubmed
    ..Potential contributions of this new reverse genetics system in the fields of influenza virus research and influenza vaccine production are discussed. ..
  44. Knutson B, Hahn S. TFIIB-related factors in RNA polymerase I transcription. Biochim Biophys Acta. 2013;1829:265-73 pubmed publisher
    ..Models for Pol I transcription initiation are reexamined in light of these recent findings. This article is part of a Special Issue entitled: Transcription by Odd Pols. ..
  45. Tan Q, Prysak M, Woychik N. Loss of the Rpb4/Rpb7 subcomplex in a mutant form of the Rpb6 subunit shared by RNA polymerases I, II, and III. Mol Cell Biol. 2003;23:3329-38 pubmed
    ..In contrast, RNA polymerase I was marginally affected...
  46. Russell J, Zomerdijk J. RNA-polymerase-I-directed rDNA transcription, life and works. Trends Biochem Sci. 2005;30:87-96 pubmed
    ..required for cell growth and division, there is mounting evidence that ribosomal DNA transcription by RNA polymerase I (pol I) not only drives cell growth via its direct role in production of the ribosomal RNA (rRNA) component ..
  47. Meier A, Thoma F. RNA polymerase I transcription factors in active yeast rRNA gene promoters enhance UV damage formation and inhibit repair. Mol Cell Biol. 2005;25:1586-95 pubmed
    ..Under exponential growth conditions about half of the genes are transcribed by RNA polymerase I (RNAP-I)...
  48. Ceribelli A, Krzyszczak M, Li Y, Ross S, Chan J, Chan E, et al. Atypical clinical presentation of a subset of patients with anti-RNA polymerase III--non-scleroderma cases associated with dominant RNA polymerase I reactivity and nucleolar staining. Arthritis Res Ther. 2011;13:R119 pubmed publisher
    ..Anti-RNAP III positive patients may not present as typical SSc and detecting anti-RNAP III may have predictive value. ..
  49. Peyroche G, Levillain E, Siaut M, Callebaut I, Schultz P, Sentenac A, et al. The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits. Proc Natl Acad Sci U S A. 2002;99:14670-5 pubmed
    A43, an essential subunit of yeast RNA polymerase I (pol I), interacts with Rrn3, a class I general transcription factor required for rDNA transcription...
  50. Navarro M, Gull K. A pol I transcriptional body associated with VSG mono-allelic expression in Trypanosoma brucei. Nature. 2001;414:759-63 pubmed
    ..Here we identify an extranucleolar body containing RNA polymerase I (pol I) that is transcriptionally active and present only in the bloodstream form of the parasite...
  51. Philippi A, Steinbauer R, Reiter A, Fath S, Leger Silvestre I, Milkereit P, et al. TOR-dependent reduction in the expression level of Rrn3p lowers the activity of the yeast RNA Pol I machinery, but does not account for the strong inhibition of rRNA production. Nucleic Acids Res. 2010;38:5315-26 pubmed publisher
    ..step in the regulation of ribosomal RNA (rRNA) gene transcription is the formation of the complex between RNA polymerase I (Pol I) and the Pol I-dependent transcription factor Rrn3p...
  52. Feng L, Li F, Zheng X, Pan W, Zhou K, Liu Y, et al. The mouse Pol I terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like RNAs with precise 3' ends in a plasmid-only-based virus rescue system. Arch Virol. 2009;154:1151-6 pubmed publisher
    ..viral RNA: one uses the self-cleaving hepatitis delta virus ribozyme (HDVR), and the other uses the murine RNA polymerase I (Pol I) terminator...
  53. Pochukalina G, Parfenov V. [The nucleolus in oocytes of multylayer mouse follicles: topography of fibrillarin, RNA polymerase I and coilin]. Tsitologiia. 2006;48:641-52 pubmed
    ..topological relationship between coilin and two key proteins of nucleolar transcription-processing machinery, RNA polymerase I and fibrillarin. RNA polymerase I rather than fibrillarin was found to be colocalized with coilin...