ornithine carbamoyltransferase

Summary

Summary: A urea cycle enzyme that catalyzes the formation of orthophosphate and L-citrulline (CITRULLINE) from CARBAMOYL PHOSPHATE and L-ornithine (ORNITHINE). Deficiency of this enzyme may be transmitted as an X-linked trait. EC 2.1.3.3.

Top Publications

  1. Luksan O, Jirsa M, Eberova J, Minks J, Treslova H, Bouckova M, et al. Disruption of OTC promoter-enhancer interaction in a patient with symptoms of ornithine carbamoyltransferase deficiency. Hum Mutat. 2010;31:E1294-303 pubmed publisher
    In a female patient with signs of ornithine carbamoyltransferase deficiency (OTCD), the only variation found was a heterozygous single nucleotide substitution c.-366A>G...
  2. Moscioni D, Morizono H, McCarter R, Stern A, Cabrera Luque J, Hoang A, et al. Long-term correction of ammonia metabolism and prolonged survival in ornithine transcarbamylase-deficient mice following liver-directed treatment with adeno-associated viral vectors. Mol Ther. 2006;14:25-33 pubmed
    ..Numerous mice, representative of all treatment groups followed for +250 days, were observed to have either nodules or discrete tumors in the liver, the etiology of which is the subject of a companion paper...
  3. El Alami M, Dubois E, Oudjama Y, Tricot C, Wouters J, Stalon V, et al. Yeast epiarginase regulation, an enzyme-enzyme activity control: identification of residues of ornithine carbamoyltransferase and arginase responsible for enzyme catalytic and regulatory activities. J Biol Chem. 2003;278:21550-8 pubmed
    In the presence of ornithine and arginine, ornithine carbamoyltransferase (OTCase) and arginase form a one-to-one enzyme complex in which the activity of OTCase is inhibited whereas arginase remains catalytically active...
  4. Sankaranarayanan R, Cherney M, Cherney L, Garen C, Moradian F, James M. The crystal structures of ornithine carbamoyltransferase from Mycobacterium tuberculosis and its ternary complex with carbamoyl phosphate and L-norvaline reveal the enzyme's catalytic mechanism. J Mol Biol. 2008;375:1052-63 pubmed
    Mycobacterium tuberculosis ornithine carbamoyltransferase (Mtb OTC) catalyzes the sixth step in arginine biosynthesis; it produces citrulline from carbamoyl phosphate (CP) and ornithine (ORN)...
  5. Ha Y, McCann M, Tuchman M, Allewell N. Substrate-induced conformational change in a trimeric ornithine transcarbamoylase. Proc Natl Acad Sci U S A. 1997;94:9550-5 pubmed
    ..As in E. coli aspartate transcarbamoylase, the 240s loop undergoes the largest conformational change upon substrate binding. The clinical implications for human OTCase deficiency are discussed. ..
  6. Mian A, McCormack W, Mane V, Kleppe S, Ng P, Finegold M, et al. Long-term correction of ornithine transcarbamylase deficiency by WPRE-mediated overexpression using a helper-dependent adenovirus. Mol Ther. 2004;10:492-9 pubmed
    ..These data represent an important approach for treating human inborn errors of hepatocyte metabolism like the UCDs that require high-level transduction and gene expression for clinical correction...
  7. Ray P, Gigarel N, Bonnefont J, Attie T, Hamamah S, Frydman N, et al. First specific preimplantation genetic diagnosis for ornithine transcarbamylase deficiency. Prenat Diagn. 2000;20:1048-54 pubmed
    ..The second cycle resulted in the birth of a baby boy devoid of the OTC mutation. This constitutes the first birth following PGD carried out by a French team...
  8. Labedan B, Boyen A, Baetens M, Charlier D, Chen P, Cunin R, et al. The evolutionary history of carbamoyltransferases: A complex set of paralogous genes was already present in the last universal common ancestor. J Mol Evol. 1999;49:461-73 pubmed
  9. Horwich A, Fenton W, Williams K, Kalousek F, Kraus J, Doolittle R, et al. Structure and expression of a complementary DNA for the nuclear coded precursor of human mitochondrial ornithine transcarbamylase. Science. 1984;224:1068-74 pubmed
    ..Both the precursor and mature forms of the OTC subunit were identified; in stable transformants, enzymatic activity was also detected. ..

More Information

Publications62

  1. Ringqvist E, Palm J, Skarin H, Hehl A, Weiland M, Davids B, et al. Release of metabolic enzymes by Giardia in response to interaction with intestinal epithelial cells. Mol Biochem Parasitol. 2008;159:85-91 pubmed publisher
    ..These results indicate that contact of Giardia with epithelial cells triggers metabolic enzyme release, which might facilitate effective colonization of the human small intestine. ..
  2. Aguilera S, López López K, Nieto Y, Garcidueñas Piña R, Hernández Guzmán G, Hernández Flores J, et al. Functional characterization of the gene cluster from Pseudomonas syringae pv. phaseolicola NPS3121 involved in synthesis of phaseolotoxin. J Bacteriol. 2007;189:2834-43 pubmed
    ..This phytotoxin inhibits the enzyme ornithine carbamoyltransferase involved in arginine biosynthesis...
  3. Xu Y, Feller G, Gerday C, Glansdorff N. Metabolic enzymes from psychrophilic bacteria: challenge of adaptation to low temperatures in ornithine carbamoyltransferase from Moritella abyssi. J Bacteriol. 2003;185:2161-8 pubmed
    The enzyme ornithine carbamoyltransferase (OTCase) of Moritella abyssi (OTCase(Mab)), a new, strictly psychrophilic and piezophilic bacterial species, was purified...
  4. Raper S, Chirmule N, Lee F, Wivel N, Bagg A, Gao G, et al. Fatal systemic inflammatory response syndrome in a ornithine transcarbamylase deficient patient following adenoviral gene transfer. Mol Genet Metab. 2003;80:148-58 pubmed
  5. Genet S, Cranston T, Middleton Price H. Mutation detection in 65 families with a possible diagnosis of ornithine carbamoyltransferase deficiency including 14 novel mutations. J Inherit Metab Dis. 2000;23:669-76 pubmed
    The high new mutation rate and the wide spectrum of mutations found in patients with ornithine carbamoyltransferase (OCT) deficiency means that direct mutation analysis is essential for providing accurate carrier detection and prenatal ..
  6. Sawada H, Suzuki F, Matsuda I, Saitou N. Phylogenetic analysis of Pseudomonas syringae pathovars suggests the horizontal gene transfer of argK and the evolutionary stability of hrp gene cluster. J Mol Evol. 1999;49:627-44 pubmed
    ..genes were then compared with another pathogenicity-related gene, argK (the phaseolotoxin-resistant ornithine carbamoyltransferase gene, which exists within the argK-tox gene cluster). All 13 strains of pv. phaseolicola and pv...
  7. Kuo L, Miller A, Lee S, Kozuma C. Site-directed mutagenesis of Escherichia coli ornithine transcarbamoylase: role of arginine-57 in substrate binding and catalysis. Biochemistry. 1988;27:8823-32 pubmed
    ..The entropy of activation is -5.5 eu for the wild-type reaction and -29 eu for the mutant reaction, accounting for a loss of 6-7 kcal/mol in the rate-determining step of the enzymic reaction.(ABSTRACT TRUNCATED AT 400 WORDS) ..
  8. de las Rivas B, Fox G, Angulo I, Ripoll M, Rodriguez H, Muñoz R, et al. Crystal structure of the hexameric catabolic ornithine transcarbamylase from Lactobacillus hilgardii: Structural insights into the oligomeric assembly and metal binding. J Mol Biol. 2009;393:425-34 pubmed publisher
    ..Additionally, the structure reveals, for the first time in the ornithine transcarbamylase family, a metal-binding site located at the 3-fold molecular symmetry axis of each trimer. ..
  9. Alam S, Bansod S, Kumar R, Sengupta N, Singh L. Differential proteomic analysis of Clostridium perfringens ATCC13124; identification of dominant, surface and structure associated proteins. BMC Microbiol. 2009;9:162 pubmed publisher
    ..Riboflavin biosynthesis protein, ornithine carbamoyltransferase, cystathionine beta-lyase, and threonine dehydratase were the predominant proteins that exhibited 2...
  10. Massant J, Wouters J, Glansdorff N. Refined structure of Pyrococcus furiosus ornithine carbamoyltransferase at 1.87 A. Acta Crystallogr D Biol Crystallogr. 2003;59:2140-9 pubmed
    Using synchrotron radiation, X-ray data have been collected from Pyrococcus furiosus ornithine carbamoyltransferase (Pfu OTCase) to a maximal resolution of 1.87 A, allowing the refinement of a previous structure at 2.7 A [Villeret et al...
  11. Naumoff D, Xu Y, Glansdorff N, Labedan B. Retrieving sequences of enzymes experimentally characterized but erroneously annotated : the case of the putrescine carbamoyltransferase. BMC Genomics. 2004;5:52 pubmed publisher
    ..ii) rediscovery of a short NH2-terminal sequence allowing to reannotate a wrongly annotated ornithine carbamoyltransferase as a putrescine carbamoyltransferase, (iii) identification of conserved motifs allowing to distinguish ..
  12. Langley D, Templeton M, Fields B, Mitchell R, Collyer C. Mechanism of inactivation of ornithine transcarbamoylase by Ndelta -(N'-Sulfodiaminophosphinyl)-L-ornithine, a true transition state analogue? Crystal structure and implications for catalytic mechanism. J Biol Chem. 2000;275:20012-9 pubmed
    ..Hence, we propose that N(delta)-(N'-sulfodiaminophosphinyl)-l-ornithine, with its three unique N-P bonds, represents a true transition state analogue for ornithine transcarbamoylases, consistent with the tight binding kinetics observed. ..
  13. Legrain C, Villeret V, Roovers M, Gigot D, Dideberg O, Pi rard A, et al. Biochemical characterisation of ornithine carbamoyltransferase from Pyrococcus furiosus. Eur J Biochem. 1997;247:1046-55 pubmed
    b>Ornithine carbamoyltransferase (OTCase) was purified to homogeneity from the hyperthermophilic archaeon Pyrococcus furiosus...
  14. Shi D, Morizono H, Ha Y, Aoyagi M, Tuchman M, Allewell N. 1.85-A resolution crystal structure of human ornithine transcarbamoylase complexed with N-phosphonacetyl-L-ornithine. Catalytic mechanism and correlation with inherited deficiency. J Biol Chem. 1998;273:34247-54 pubmed
  15. López López K, Hernández Flores J, Cruz Aguilar M, Alvarez Morales A. In Pseudomonas syringae pv. phaseolicola, expression of the argK gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase, is regulated indirectly by temperature and directly by a precursor resembling carbamoylphosphate. J Bacteriol. 2004;186:146-53 pubmed
    ..synthesizes a non-host-specific toxin, phaseolotoxin, and also synthesizes a phaseolotoxin-resistant ornithine carbamoyltransferase (ROCT) to protect itself from its own toxin...
  16. Lindgren V, de Martinville B, Horwich A, Rosenberg L, Francke U. Human ornithine transcarbamylase locus mapped to band Xp21.1 near the Duchenne muscular dystrophy locus. Science. 1984;226:698-700 pubmed
  17. Bell P, Moscioni A, McCarter R, Wu D, Gao G, Hoang A, et al. Analysis of tumors arising in male B6C3F1 mice with and without AAV vector delivery to liver. Mol Ther. 2006;14:34-44 pubmed
    ..It appears that AAV vectors alone do not contribute to the formation of tumors in these strains of mice although the expression of LacZ alone or in combination with vector may be problematic...
  18. Chun J, Lee M. Cloning of the argF gene encoding the ornithine carbamoyltransferase from Corynebacterium glutamicum. Mol Cells. 1999;9:333-7 pubmed
    ..coli auxotroph carrying the argF gene revealed that the cloned gene indeed codes for OTCase. Analysis of the amino acid sequence of the predicted protein revealed a strong similarity to the corresponding protein of other bacteria...
  19. Sawada H, Takeuchi T, Matsuda I. Comparative analysis of Pseudomonas syringae pv. actinidiae and pv. phaseolicola based on phaseolotoxin-resistant ornithine carbamoyltransferase gene (argK) and 16S-23S rRNA intergenic spacer sequences. Appl Environ Microbiol. 1997;63:282-8 pubmed
    ..plants, are known as phaseolotoxin producers, and the former possesses phaseolotoxin-resistant ornithine carbamoyltransferase (ROCT) which confers resistance to the toxin...
  20. Villeret V, Clantin B, Tricot C, Legrain C, Roovers M, Stalon V, et al. The crystal structure of Pyrococcus furiosus ornithine carbamoyltransferase reveals a key role for oligomerization in enzyme stability at extremely high temperatures. Proc Natl Acad Sci U S A. 1998;95:2801-6 pubmed
    The Pyrococcus furiosus (PF) ornithine carbamoyltransferase (OTCase; EC 2.1.3.3) is an extremely heat-stable enzyme that maintains about 50% of its activity after heat treatment for 60 min at 100 degrees C...
  21. Massant J, Glansdorff N. Metabolic channelling of carbamoyl phosphate in the hyperthermophilic archaeon Pyrococcus furiosus: dynamic enzyme-enzyme interactions involved in the formation of the channelling complex. Biochem Soc Trans. 2004;32:306-9 pubmed
    ..By physically interacting with each other, CKase (carbamate kinase) and OTCase (ornithine carbamoyltransferase) prevent thermodenaturation of CP in the aqueous cytoplasmic environment...
  22. Yamaguchi S, Brailey L, Morizono H, Bale A, Tuchman M. Mutations and polymorphisms in the human ornithine transcarbamylase (OTC) gene. Hum Mutat. 2006;27:626-32 pubmed
    ..5% of the genomic sequence. In addition, a phenocopy of OTC deficiency caused by mutations in another unknown gene cannot be excluded...
  23. Hughes M, Moore J, Lane J, Wilson R, Pribul P, Younes Z, et al. Identification of major outer surface proteins of Streptococcus agalactiae. Infect Immun. 2002;70:1254-9 pubmed
    ..agalactiae, were sequenced and cloned. These were ornithine carbamoyltransferase, phosphoglycerate kinase, nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase, purine ..
  24. Yoo H, Kim G, Lee D. Identification of new mutations in the ornithine transcarbamylase (OTC) gene in Korean families. J Inherit Metab Dis. 1996;19:31-42 pubmed
    ..This missense mutation has been described previously and is known to eliminate a TaqI recognition site in exon 5...
  25. Winterhoff N, Goethe R, Gruening P, Rohde M, Kalisz H, Smith H, et al. Identification and characterization of two temperature-induced surface-associated proteins of Streptococcus suis with high homologies to members of the Arginine Deiminase system of Streptococcus pyogenes. J Bacteriol. 2002;184:6768-76 pubmed
    ..sequence analysis of the two proteins indicated homologies of the 47-kDa protein with an ornithine carbamoyltransferase (OCT) from Streptococcus pyogenes and of the 53-kDa protein with the streptococcal acid glycoprotein (..
  26. Cohen N, Cheung C, Raijman L. Altered enzyme activities and citrulline synthesis in liver mitochondria from ornithine carbamoyltransferase-deficient sparse-furash mice. Biochem J. 1989;257:251-7 pubmed
    Male mice carrying the spfash mutation have 5-10% of the normal activity of ornithine carbamoyltransferase, yet are only slightly hyperammonaemic and develop quite well...
  27. Takahashi M, Hood D. Protein import into subsarcolemmal and intermyofibrillar skeletal muscle mitochondria. Differential import regulation in distinct subcellular regions. J Biol Chem. 1996;271:27285-91 pubmed
  28. Yu W, Lin Y, Yao J, Huang W, Lei Q, Xiong Y, et al. Lysine 88 acetylation negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals. J Biol Chem. 2009;284:13669-75 pubmed publisher
    b>Ornithine carbamoyltransferase (OTC) is a key enzyme in the urea cycle to detoxify ammonium produced from amino acid catabolism...
  29. Cunningham S, Spinoulas A, Carpenter K, Wilcken B, Kuchel P, Alexander I. AAV2/8-mediated correction of OTC deficiency is robust in adult but not neonatal Spf(ash) mice. Mol Ther. 2009;17:1340-6 pubmed publisher
    ..This loss of expression in the neonatal liver is the consequence of hepatocellular proliferation and presents an added challenge to human therapy...
  30. Zúñiga M, Pérez G, Gonzalez Candelas F. Evolution of arginine deiminase (ADI) pathway genes. Mol Phylogenet Evol. 2002;25:429-44 pubmed
    ..Furthermore, the molecular phylogenetic analysis of OTC suggests a new classification of these genes into three subfamilies. ..
  31. Clantin B, Tricot C, Lonhienne T, Stalon V, Villeret V. Probing the role of oligomerization in the high thermal stability of Pyrococcus furiosus ornithine carbamoyltransferase by site-specific mutants. Eur J Biochem. 2001;268:3937-42 pubmed
    The Pyrococcus furiosus ornithine carbamoyltransferase (OTCase) is extremely heat stable and maintains 50% of its catalytic activity after 60 min at 100 degrees C...
  32. Shi D, Gallegos R, DePonte J, Morizono H, Yu X, Allewell N, et al. Crystal structure of a transcarbamylase-like protein from the anaerobic bacterium Bacteroides fragilis at 2.0 A resolution. J Mol Biol. 2002;320:899-908 pubmed publisher
    ..A similar protein has been found in Xylella fastidiosa, a bacterium that infects grapes, citrus and other plants...
  33. Kanazawa M, Terada K, Kato S, Mori M. HSDJ, a human homolog of DnaJ, is farnesylated and is involved in protein import into mitochondria. J Biochem. 1997;121:890-5 pubmed
    ..Coexpression of HSDJ or C394S resulted in slight retardation of the pOTC processing. These results indicate that HSDJ is involved in an early step(s) of protein import into mitochondria. ..
  34. Morizono H, Tuchman M, Rajagopal B, McCann M, Listrom C, Yuan X, et al. Expression, purification and kinetic characterization of wild-type human ornithine transcarbamylase and a recurrent mutant that produces 'late onset' hyperammonaemia. Biochem J. 1997;322 ( Pt 2):625-31 pubmed
    ..Its reduced affinity for L-ornithine, which in turn results in lower activity at physiological concentrations of ornithine, as well as its reduced stability, may contribute to the clinical effects that it produces. ..
  35. Crabeel M, Messenguy F, Lacroute F, Glansdorff N. Cloning arg3, the gene for ornithine carbamoyltransferase from Saccharomyces cerevisiae: expression in Escherichia coli requires secondary mutations; production of plasmid beta-lactamase in yeast. Proc Natl Acad Sci U S A. 1981;78:5026-30 pubmed
    The yeast arg3 gene, coding for ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase, EC 2.1.3.3), has been cloned on a hybrid pBR322-2-micrometers plasmid...
  36. Baur H, Stalon V, Falmagne P, Luethi E, Haas D. Primary and quaternary structure of the catabolic ornithine carbamoyltransferase from Pseudomonas aeruginosa. Extensive sequence homology with the anabolic ornithine carbamoyltransferases of Escherichia coli. Eur J Biochem. 1987;166:111-7 pubmed
    ..arcB gene from Pseudomonas aeruginosa strain PAO and we have purified the arcB product, the catabolic ornithine carbamoyltransferase (EC 2.1.3.3), to apparent homogeneity from the same strain...
  37. Jahn O, Sauerstein J, Reuter G. Characterization of two ornithine carbamoyltransferases from Pseudomonas syringae pv. phaseolicola, the producer of phaseolotoxin. Arch Microbiol. 1987;147:174-8 pubmed
    ..Both enzymes possess only an anabolic function. OCT 1 is highly inhibited by low concentrations of phaseolotoxin and Orn-P(O)(NH2)-NH-SO3H, OCT 2 is insensitive to both compounds. The inhibition of OCT 1 is reversible. ..
  38. Tuchman M, Jaleel N, Morizono H, Sheehy L, Lynch M. Mutations and polymorphisms in the human ornithine transcarbamylase gene. Hum Mutat. 2002;19:93-107 pubmed
    ..Even with sequencing of the entire reading frame and exon/intron boundaries, only about 80% of the mutations are detected in patients with proven OTC deficiency. The remaining probably occur within the introns or in regulatory domains...
  39. Arakawa H, Amaya Y, Mori M. The NH2-terminal 14-16 amino acids of mitochondrial and bacterial thiolases can direct mature ornithine carbamoyltransferase into mitochondria. J Biochem. 1990;107:160-4 pubmed
    ..16 residues (MALLRGVFIVAAKRTP) of the mitochondrial thiolase fused to the mature portion of rat ornithine carbamoyltransferase [EC 2.1.3...
  40. Murakami T, Takiguchi M, Inomoto T, Yamamura K, Mori M. Tissue- and developmental stage-specific expression of the rat ornithine carbamoyltransferase gene in transgenic mice. Dev Genet. 1989;10:393-401 pubmed
    Rat ornithine carbamoyltransferase (OCT; EC 2.1.3.3) is encoded by a large gene of 75 kilobases. Expression of this gene is restricted to the liver and small intestine, and there is an increase in expression late in gestation...
  41. Hernández Flores J, López López K, Garcidueñas Piña R, Jofre Garfias A, Alvarez Morales A. The global arginine regulator ArgR controls expression of argF in Pseudomonas syringae pv. phaseolicola but is not required for the synthesis of phaseolotoxin or for the regulated expression of argK. J Bacteriol. 2004;186:3653-5 pubmed
    In Pseudomonas syringae pv. phaseolicola the enzyme ornithine carbamoyltransferase (OCTase), encoded by argF, is negatively regulated by argR, similar to what has been reported for Pseudomonas aeruginosa...
  42. Villeret V, Tricot C, Stalon V, Dideberg O. Crystal structure of Pseudomonas aeruginosa catabolic ornithine transcarbamoylase at 3.0-A resolution: a different oligomeric organization in the transcarbamoylase family. Proc Natl Acad Sci U S A. 1995;92:10762-6 pubmed
    ..However, both enzymes display homotropic and heterotropic cooperativity. ..
  43. Curis E, Nicolis I, Moinard C, Osowska S, Zerrouk N, Benazeth S, et al. Almost all about citrulline in mammals. Amino Acids. 2005;29:177-205 pubmed
    ..Free citrulline metabolism involves three key enzymes: NO synthase (NOS) and ornithine carbamoyltransferase (OCT) which produce citrulline, and argininosuccinate synthetase (ASS) that converts it into ..
  44. Cheng P, Lam T, Lam W, Tsui S, Cheng A, Lo W, et al. Pegylated recombinant human arginase (rhArg-peg5,000mw) inhibits the in vitro and in vivo proliferation of human hepatocellular carcinoma through arginine depletion. Cancer Res. 2007;67:309-17 pubmed
    ..Thus, our data suggest that rhArg-peg(5,000mw) is a novel agent for effective cancer therapy. ..
  45. Díaz Muñoz M, Hernández Muñoz R. Molecular and biochemical features of the mitochondrial enzyme ornithine transcarbamylase: a possible new role as a signaling factor. Curr Med Chem. 2010;17:2253-60 pubmed
    ..In this situation, extracellular and circulating hepatic OTC could be playing a different role, possibly functioning as a signaling molecule. ..
  46. Falmagne P, Portetelle D, Stalon V. Immunological and structural relatedness of catabolic ornithine carbamoyltransferases and the anabolic enzymes of enterobacteria. J Bacteriol. 1985;161:714-9 pubmed
    Purified catabolic ornithine carbamoyltransferase of Pseudomonas putida and anabolic ornithine carbamoyltransferase (argF product) of Escherichia coli K-12 were used to prepare antisera...
  47. Aoki Y, Sunaga H, Suzuki K. A cadmium-binding protein in rat liver identified as ornithine carbamoyltransferase. Biochem J. 1988;250:735-42 pubmed
    ..Biochem. 157, 117-122]. It was characterized and identified as ornithine carbamoyltransferase (OCTase, EC 2.1.3.3) on the basis of coincidence of their physicochemical and enzymological features...
  48. Baur H, Tricot C, Stalon V, Haas D. Converting catabolic ornithine carbamoyltransferase to an anabolic enzyme. J Biol Chem. 1990;265:14728-31 pubmed
    Pseudomonas aeruginosa has an anabolic and a catabolic ornithine carbamoyltransferase (OTCase)...
  49. Hatziloukas E, Panopoulos N. Origin, structure, and regulation of argK, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicola, and functional expression of argK in transgenic tobacco. J Bacteriol. 1992;174:5895-909 pubmed
    ..which functions as a chlorosis-inducing toxin in the bean halo blight disease by inhibiting ornithine carbamoyltransferase (OCT)...
  50. Mosqueda G, Van den Broeck G, Saucedo O, Bailey A, Alvarez Morales A, Herrera Estrella L. Isolation and characterization of the gene from Pseudomonas syringae pv. phaseolicola encoding the phaseolotoxin-insensitive ornithine carbamoyltransferase. Mol Gen Genet. 1990;222:461-6 pubmed
    The gene coding for the phaseolotoxin-insensitive ornithine carbamoyltransferase (OCTase) from Pseudomonas syringae pv. phaseolicola has been cloned and sequenced...
  51. Messenguy F. Regulation of arginine biosynthesis in Saccharomyces cerevisiae: isolation of a cis-dominant, constitutive mutant for ornithine carbamoyltransferase synthesis. J Bacteriol. 1976;128:49-55 pubmed
    A cis-dominant mutation linked to argF, the structural gene specifying ornithine carbamoyltransferase, and affecting the control of the synthesis of this enzyme has been obtained...
  52. Shah K, Chiu W, Scalea T, Carlson D. Detrimental effects of rapid fluid resuscitation on hepatocellular function and survival after hemorrhagic shock. Shock. 2002;18:242-7 pubmed
    ..Plasma ornithine carbamoyltransferase (OCT), lactate, and creatinine were measured as indices of hepatocellular injury, anaerobic metabolism,..
  53. McDowall S, van Heeswijck R, Hoogenraad N. Site-directed mutagenesis of Arg60 and Cys271 in ornithine transcarbamylase from rat liver. Protein Eng. 1990;4:73-7 pubmed
    ..The small loss in binding energy and relatively high residual catalytic activity of the mutant strongly suggests that a number of other residues are involved in the binding of ornithine.(ABSTRACT TRUNCATED AT 250 WORDS) ..