uridine phosphorylase

Summary

Summary: An enzyme that catalyzes the transfer of ribose from uridine to orthophosphate, forming uracil and ribose 1-phosphate.

Top Publications

  1. Cao D, Pizzorno G. Uridine phosophorylase: an important enzyme in pyrimidine metabolism and fluoropyrimidine activation. Drugs Today (Barc). 2004;40:431-43 pubmed
    b>Uridine phosphorylase (UPase) is an enzyme that can convert uridine to uracil...
  2. Kong X, Fan H, Liu X, Wang R, Liang J, Gupta N, et al. Peroxisome proliferator-activated receptor gamma coactivator-1alpha enhances antiproliferative activity of 5'-deoxy-5-fluorouridine in cancer cells through induction of uridine phosphorylase. Mol Pharmacol. 2009;76:854-60 pubmed publisher
    ..b>Uridine phosphorylase (UPase) catalyzes the reversible conversion of uridine into uracil and contributes to the antineoplastic ..
  3. Burling F, Kniewel R, Buglino J, Chadha T, Beckwith A, Lima C. Structure of Escherichia coli uridine phosphorylase at 2.0 A. Acta Crystallogr D Biol Crystallogr. 2003;59:73-6 pubmed
    The 2.0 A crystal structure has been determined for Escherichia coli uridine phosphorylase (UP), an essential enzyme in nucleotide biosynthesis that catalyzes the phosphorolytic cleavage of the C-N glycosidic bond of uridine to ribose-1-..
  4. Johansson M. Identification of a novel human uridine phosphorylase. Biochem Biophys Res Commun. 2003;307:41-6 pubmed
    b>Uridine phosphorylase catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate...
  5. Deneen B, Hamidi H, Denny C. Functional analysis of the EWS/ETS target gene uridine phosphorylase. Cancer Res. 2003;63:4268-74 pubmed
    ..b>Uridine phosphorylase is a gene that is up-regulated by structurally distinct EWS/ETS fusions...
  6. Cuq P, Rouquet C, Evrard A, Ciccolini J, Vian L, Cano J. Fluoropyrimidine sensitivity of human MCF-7 breast cancer cells stably transfected with human uridine phosphorylase. Br J Cancer. 2001;84:1677-80 pubmed
    The relationship between uridine phosphorylase (UP) expression level in cancer cells and the tumour sensitivity to fluoropyrimidines is unclear...
  7. Geromichalos G, Geromichalou E, Camoutsis C, Kontos M, Dalezis P, Papageorgiou A, et al. In silico/in vitro study of hybrid D-modified steroidal alkylator anticancer activity using uridine phosphorylase as target protein. Anticancer Res. 2011;31:831-42 pubmed
    ..steroidal esters were synthesized and tested in vitro against human pancreatic cancer cells expressing uridine phosphorylase (UPase)...
  8. Silva R, Kipp D, Schramm V. Constrained bonding environment in the Michaelis complex of Trypanosoma cruzi uridine phosphorylase. Biochemistry. 2012;51:6715-7 pubmed
    The transition state for the Trypanosoma cruzi uridine phosphorylase (TcUP) reaction has an expanded S(N)2 character...
  9. Al Safarjalani O, Rais R, Shi J, Schinazi R, Naguib F, El Kouni M. Modulation of 5-fluorouracil host-toxicity and chemotherapeutic efficacy against human colon tumors by 5-(Phenylthio)acyclouridine, a uridine phosphorylase inhibitor. Cancer Chemother Pharmacol. 2006;58:692-8 pubmed
    ..PTAU is a potent and specific inhibitor of uridine phosphorylase (UrdPase, EC 2.4.2.3), the enzyme responsible for uridine catabolism...

More Information

Publications62

  1. Cao D, Leffert J, McCabe J, Kim B, Pizzorno G. Abnormalities in uridine homeostatic regulation and pyrimidine nucleotide metabolism as a consequence of the deletion of the uridine phosphorylase gene. J Biol Chem. 2005;280:21169-75 pubmed
    We report in the present study the critical role of uridine phosphorylase (UPase) in uridine homeostatic regulation and pyrimidine nucleotide metabolism, employing newly developed UPase-/- mice...
  2. Choi J, Yoo B, Shin C, Ryu M, Ryu J, El Kouni M, et al. Uridine prevents the glucose deprivation-induced death of immunostimulated astrocytes via the action of uridine phosphorylase. Neurosci Res. 2006;56:111-8 pubmed
    ..mRNA and protein expression for uridine phosphorylase, an enzyme catalyzing reversible phosphorolysis of uridine, were observed in rat brain as well as primary ..
  3. Roosild T, Castronovo S. Active site conformational dynamics in human uridine phosphorylase 1. PLoS ONE. 2010;5:e12741 pubmed publisher
    b>Uridine phosphorylase (UPP) is a central enzyme in the pyrimidine salvage pathway, catalyzing the reversible phosphorolysis of uridine to uracil and ribose-1-phosphate...
  4. Caradoc Davies T, Cutfield S, Lamont I, Cutfield J. Crystal structures of Escherichia coli uridine phosphorylase in two native and three complexed forms reveal basis of substrate specificity, induced conformational changes and influence of potassium. J Mol Biol. 2004;337:337-54 pubmed
    b>Uridine phosphorylase (UP) is a key enzyme in the pyrimidine salvage pathway that catalyses the reversible phosphorolysis of uridine to uracil and ribose 1-phosphate...
  5. Zhang Y, Repa J, Inoue Y, Hayhurst G, Gonzalez F, Mangelsdorf D. Identification of a liver-specific uridine phosphorylase that is regulated by multiple lipid-sensing nuclear receptors. Mol Endocrinol. 2004;18:851-62 pubmed
    ..L-UrdPase encodes a previously uncharacterized protein with similarity to an intestine-specific uridine phosphorylase. Enzymatic assays confirmed that L-UrdPase has uridine phosphorylase activity...
  6. Haraguchi M, Tsujimoto H, Fukushima M, Higuchi I, Kuribayashi H, Utsumi H, et al. Targeted deletion of both thymidine phosphorylase and uridine phosphorylase and consequent disorders in mice. Mol Cell Biol. 2002;22:5212-21 pubmed
    ..Murine uridine phosphorylase (UP), unlike human UP, cleaves thymidine, as well as uridine...
  7. Pizzorno G, Cao D, Leffert J, Russell R, Zhang D, Handschumacher R. Homeostatic control of uridine and the role of uridine phosphorylase: a biological and clinical update. Biochim Biophys Acta. 2002;1587:133-44 pubmed
    ..uridine in plasma and tissues is tightly regulated by cellular transport mechanisms and by the activity of uridine phosphorylase (UPase), responsible for the reversible phosphorolysis of uridine to uracil...
  8. Tozzi M, Camici M, Mascia L, Sgarrella F, Ipata P. Pentose phosphates in nucleoside interconversion and catabolism. FEBS J. 2006;273:1089-101 pubmed
    ..acting catabolically, is either anabolized for pyrimidine salvage and 5-fluorouracil activation, with uridine phosphorylase acting anabolically, or recycled for nucleoside and base interconversion; (b) the nucleosides can be ..
  9. Bu W, Settembre E, El Kouni M, Ealick S. Structural basis for inhibition of Escherichia coli uridine phosphorylase by 5-substituted acyclouridines. Acta Crystallogr D Biol Crystallogr. 2005;61:863-72 pubmed
    b>Uridine phosphorylase (UP) catalyzes the reversible phosphorolysis of uridine to uracil and ribose 1-phosphate and is a key enzyme in the pyrimidine-salvage pathway. Escherichia coli UP is structurally homologous to E...
  10. Renck D, Ducati R, Palma M, Santos D, Basso L. The kinetic mechanism of human uridine phosphorylase 1: Towards the development of enzyme inhibitors for cancer chemotherapy. Arch Biochem Biophys. 2010;497:35-42 pubmed publisher
    b>Uridine phosphorylase (UP) is a key enzyme in the pyrimidine salvage pathway, catalyzing the reversible phosphorolysis of uridine to uracil and ribose-1-phosphate (R1P)...
  11. Kawamura K, Takiguchi N, Wada A, Takenobu H, Kimura H, Soda H, et al. Up-regulated expression of the uridine phosphorylase gene in human gastric tumors is correlated with a favorable prognosis. Anticancer Res. 2006;26:4647-51 pubmed
    b>Uridine phosphorylase (UP) is one of the enzymes involved in 5-fluorouracil (5-FU) activation. The expression was compared in paired specimens from cancerous and non-cancerous regions of gastric, colon and lung cancer patients...
  12. Lashkov A, Zhukhlistova N, Gabdoulkhakov A, Shtil A, Efremov R, Betzel C, et al. The X-ray structure of Salmonella typhimurium uridine nucleoside phosphorylase complexed with 2,2'-anhydrouridine, phosphate and potassium ions at 1.86 A resolution. Acta Crystallogr D Biol Crystallogr. 2010;66:51-60 pubmed publisher
    ..86 A resolution (R(cryst) = 17.6%, R(free) = 20.6%). The complex of human uridine phosphorylase I (HUPhI) with 2,2'-anhydrouridine was modelled using a computational approach...
  13. Cao D, Russell R, Zhang D, Leffert J, Pizzorno G. Uridine phosphorylase (-/-) murine embryonic stem cells clarify the key role of this enzyme in the regulation of the pyrimidine salvage pathway and in the activation of fluoropyrimidines. Cancer Res. 2002;62:2313-7 pubmed
    We have reported the elevation of uridine phosphorylase (UPase) in many solid tumors and the presence of a variant phosphorolytic activity in breast cancer tissues (M. Liu et al., Cancer Res., 58: 5418-5424, 1998)...
  14. Choi J, Shin C, Choi M, Yoon S, Ryu J, Lee J, et al. Uridine protects cortical neurons from glucose deprivation-induced death: possible role of uridine phosphorylase. J Neurotrauma. 2008;25:695-707 pubmed publisher
    ..b>Uridine phosphorylase (UPase), an enzyme catalyzing the reversible phosphorylation of uridine, was involved in this effect...
  15. Roosild T, Castronovo S, Fabbiani M, Pizzorno G. Implications of the structure of human uridine phosphorylase 1 on the development of novel inhibitors for improving the therapeutic window of fluoropyrimidine chemotherapy. BMC Struct Biol. 2009;9:14 pubmed publisher
    b>Uridine phosphorylase (UPP) is a key enzyme of pyrimidine salvage pathways, catalyzing the reversible phosphorolysis of ribosides of uracil to nucleobases and ribose 1-phosphate...
  16. Dontsova M, Gabdoulkhakov A, Molchan O, Lashkov A, Garber M, Mironov A, et al. Preliminary investigation of the three-dimensional structure of Salmonella typhimurium uridine phosphorylase in the crystalline state. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005;61:337-40 pubmed publisher
    b>Uridine phosphorylase (UPh) catalyzes the phosphorolytic cleavage of the C-N glycosidic bond of uridine to ribose 1-phosphate and uracil in the pyrimidine-salvage pathway...
  17. Morgunova EYu -, Mikhailov A, Popov A, Blagova E, Smirnova E, Vainshtein B, et al. Atomic structure at 2.5 A resolution of uridine phosphorylase from E. coli as refined in the monoclinic crystal lattice. FEBS Lett. 1995;367:183-7 pubmed
    b>Uridine phosphorylase from E. coli (Upase) has been crystallized using vapor diffusion technique in a new monoclinic crystal form. The structure was determined by the molecular replacement method at 2.5 A resolution...
  18. Larson E, Mudeppa D, Gillespie J, Mueller N, Napuli A, Arif J, et al. The crystal structure and activity of a putative trypanosomal nucleoside phosphorylase reveal it to be a homodimeric uridine phosphorylase. J Mol Biol. 2010;396:1244-59 pubmed publisher
    ..We suggest that this recognizable feature may aid in proper annotation of the substrate specificity of enzymes in the NP family. ..
  19. Al Safarjalani O, Zhou X, Naguib F, Shi J, Schinazi R, el Kouni M. Enhancement of the bioavailability of oral uridine by coadministration of 5-(phenylthio)acyclouridine, a uridine phosphorylase inhibitor: implications for uridine rescue regimens in chemotherapy. Cancer Chemother Pharmacol. 2001;48:389-97 pubmed
    ..PTAU is a new potent and specific inhibitor of uridine phosphorylase (UrdPase, EC 2.4.2.3), the enzyme responsible for uridine catabolism...
  20. Paul D, O Leary S, Rajashankar K, Bu W, Toms A, Settembre E, et al. Glycal formation in crystals of uridine phosphorylase. Biochemistry. 2010;49:3499-509 pubmed publisher
    b>Uridine phosphorylase is a key enzyme in the pyrimidine salvage pathway. This enzyme catalyzes the reversible phosphorolysis of uridine to uracil and ribose 1-phosphate (or 2'-deoxyuridine to 2'-deoxyribose 1-phosphate)...
  21. Russell R, Cao D, Zhang D, Handschumacher R, Pizzorno G. Uridine phosphorylase association with vimentin. Intracellular distribution and localization. J Biol Chem. 2001;276:13302-7 pubmed
    b>Uridine phosphorylase (UPase), a key enzyme in the pyrimidine salvage pathway, is associated with the intermediate filament protein vimentin, in NIH 3T3 fibroblasts and colon 26 cells...
  22. Cao D, Ziemba A, McCabe J, Yan R, Wan L, Kim B, et al. Differential expression of uridine phosphorylase in tumors contributes to an improved fluoropyrimidine therapeutic activity. Mol Cancer Ther. 2011;10:2330-9 pubmed publisher
    Abrogation of uridine phosphorylase (UPase) leads to abnormalities in pyrimidine metabolism and host protection against 5-fluorouracil (5-FU) toxicity...
  23. Im Y, Shin H, Kim H, Jeong S, Kim S, Kim Y, et al. Enhanced cytotoxicity of 5-FU by bFGF through up-regulation of uridine phosphorylase 1. Mol Cells. 2009;28:119-24 pubmed publisher
    ..factors that correlate with the response to 5-FU-based chemotherapy have been proposed to include uridine phosphorylase (UPP), thymidine phosphorylase (TPP), p53 and microsatellite instability...
  24. Watanabe S, Uchida T. Cloning and expression of human uridine phosphorylase. Biochem Biophys Res Commun. 1995;216:265-72 pubmed
    Using a mouse cDNA probe we have identified a human uridine phosphorylase cDNA clone from the cDNA library of a human colorectal tumor cell line, HCT116...
  25. Mitterbauer R, Karl T, Adam G. Saccharomyces cerevisiae URH1 (encoding uridine-cytidine N-ribohydrolase): functional complementation by a nucleoside hydrolase from a protozoan parasite and by a mammalian uridine phosphorylase. Appl Environ Microbiol. 2002;68:1336-43 pubmed
    ..In contrast, uridine phosphorylase is the key enzyme of pyrimidine salvage in mammalian hosts and many other organisms...
  26. Fuchs J, Warner H. Isolation of an Escherichia coli mutant deficient in glutathione synthesis. J Bacteriol. 1975;124:140-8 pubmed
    ..This suggests that one role of glutathione in the cell is to maintain at least this one protein in an active state. We propose the designation gshB for the gene coding for glutathione synthetase. ..
  27. Pula G, Garonna E, Dunn W, Hirano M, Pizzorno G, Campanella M, et al. Paracrine stimulation of endothelial cell motility and angiogenesis by platelet-derived deoxyribose-1-phosphate. Arterioscler Thromb Vasc Biol. 2010;30:2631-8 pubmed publisher
    ..This phenomenon was ablated by genetic silencing of dRP-generating uridine phosphorylase (UP) and thymidine phosphorylase (TP) or pharmacological inhibition of UP and restored by exogenous dRP...
  28. Beck D, O Donovan G. Pathways of pyrimidine salvage in Pseudomonas and former Pseudomonas: detection of recycling enzymes using high-performance liquid chromatography. Curr Microbiol. 2008;56:162-7 pubmed
    ..Cod: cytosine + H2O --> uracil + NH3], cytidine deaminase [Cdd: cytidine + H2O --> uridine + NH3], uridine phosphorylase [Udp: uridine + Pi <--> uracil + ribose - 1 - P], nucleoside hydrolase [Nuh: purine/pyrimidine ..
  29. Mihara H, Hidese R, Yamane M, Kurihara T, Esaki N. The iscS gene deficiency affects the expression of pyrimidine metabolism genes. Biochem Biophys Res Commun. 2008;372:407-11 pubmed publisher
    ..By using differential screening strategies, we identified 2 pyrimidine salvage enzymes, namely, uridine phosphorylase and cytidine deaminase, which were down-regulated in the iscS mutant...
  30. Miyashita H, Takebayashi Y, Eliason J, Fujimori F, Nitta Y, Sato A, et al. Uridine phosphorylase is a potential prognostic factor in patients with oral squamous cell carcinoma. Cancer. 2002;94:2959-66 pubmed
    b>Uridine phosphorylase (UPase) catalyzes the reversible phosphorolysis of uridine to uracil. The expression levels and the enzymatic activity of UPase are reported to be higher in human solid tumors than in adjacent normal tissues...
  31. Balestri F, Barsotti C, Lutzemberger L, Camici M, Ipata P. Key role of uridine kinase and uridine phosphorylase in the homeostatic regulation of purine and pyrimidine salvage in brain. Neurochem Int. 2007;51:517-23 pubmed
    ..uridine is maintained by uridine kinase, catalyzing the first step of UTP and CTP salvage synthesis, and uridine phosphorylase, catalyzing the first step of uridine degradation to beta-alanine in liver...
  32. Le T, Ziemba A, Urasaki Y, Hayes E, Brotman S, Pizzorno G. Disruption of uridine homeostasis links liver pyrimidine metabolism to lipid accumulation. J Lipid Res. 2013;54:1044-57 pubmed publisher
    ..in this study an intrinsic link between pyrimidine metabolism and liver lipid accumulation utilizing a uridine phosphorylase 1 transgenic mouse model UPase1-TG...
  33. Silva R, Schramm V. Uridine phosphorylase from Trypanosoma cruzi: kinetic and chemical mechanisms. Biochemistry. 2011;50:9158-66 pubmed publisher
    The reversible phosphorolysis of uridine to generate uracil and ribose 1-phosphate is catalyzed by uridine phosphorylase and is involved in the pyrimidine salvage pathway...
  34. Fox B, Bzik D. Avirulent uracil auxotrophs based on disruption of orotidine-5'-monophosphate decarboxylase elicit protective immunity to Toxoplasma gondii. Infect Immun. 2010;78:3744-52 pubmed publisher
    ..Additionally, to functionally address the role of the pyrimidine salvage pathway, the uridine phosphorylase (UP) salvage activity was knocked out and a double knockout of UP and OMPDC was also constructed...
  35. García Diaz B, Garone C, Barca E, Mojahed H, Gutierrez P, Pizzorno G, et al. Deoxynucleoside stress exacerbates the phenotype of a mouse model of mitochondrial neurogastrointestinal encephalopathy. Brain. 2014;137:1337-49 pubmed publisher
    ..Thymidine phosphorylase and uridine phosphorylase double knockout mice recapitulated several features of these patients including thymidine phosphorylase ..
  36. Hachicha M, Kammoun T, Ben Romdhane W, Ben Abdallah R, Mahfoudh A, Kammoun K, et al. [Vasculitis with renal involvement and antineutrophil cytoplasmic antibodies (ANCA) in a child receiving benzylthiouracil]. Nephrol Ther. 2007;3:147-51 pubmed
    ..These results permitted to announce hypothesis that benzylthiouracil was implicated in development of vasculitis associated to ANCA. ..
  37. Brikun I, Suziedelis K, Stemmann O, Zhong R, Alikhanian L, Linkova E, et al. Analysis of CRP-CytR interactions at the Escherichia coli udp promoter. J Bacteriol. 1996;178:1614-22 pubmed
    ..regulation by selecting and characterizing spontaneous constitutive mutations in the promoter of the udp (uridine phosphorylase) gene, one of the genes most strongly regulated by CytR...
  38. Katsumata K, Tomioka H, Sumi T, Yamashita S, Takagi M, Kato F, et al. Correlation between clinicopathologic factors and kinetics of metabolic enzymes for 5-fluorouracil given to patients with colon carcinoma by two different dosage regimens. Cancer Chemother Pharmacol. 2003;51:155-60 pubmed
    ..The activities of orotate phosphoribosyl transferase (OPRT), thymidine phosphorylase (TP), uridine phosphorylase (UP), and dihydropyrimidine dehydrogenase (DPD) were measured by separation on ion-exchange filter paper, ..
  39. Mironov A, Nechaeva G, Sukhodolets V. [Interaction of negative (CytT) and positive (cAMP-CRP) regulation in the promoter region of the uridine phosphorylase (udp) gene in Escherichia coli K-12]. Genetika. 1989;25:438-47 pubmed
    Interaction of negative (CytR) and positive (cAMP-CRP) control in the promoter region of the uridine phosphorylase (udp) gene of Escherichia coli has been studied by using udp-lac operon fusions in which the structural lacZ gene is ..
  40. Lashkov A, Gabdulkhakov A, Prokofev I, Seregina T, Sotnichenko S, Lyashenko A, et al. Expression, purification, crystallization and preliminary X-ray structure analysis of Vibrio cholerae uridine phosphorylase in complex with thymidine. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012;68:1394-7 pubmed publisher
    A high-resolution structure of the complex of Vibrio cholerae uridine phosphorylase (VchUPh) with its physiological ligand thymidine is important in order to determine the mechanism of the substrate specificity of the enzyme and for the ..
  41. Domingo P, Torres Torronteras J, Pomar V, Giralt M, Domingo J, Gutierrez M, et al. Uridine metabolism in HIV-1-infected patients: effect of infection, of antiretroviral therapy and of HIV-1/ART-associated lipodystrophy syndrome. PLoS ONE. 2010;5:e13896 pubmed publisher
    ..25 (2.55-4.15) µmol/l/l (P = 0.0066). The expression of uridine cytidine kinase and uridine phosphorylase genes was significantly decreased in all groups of patients with respect to controls...
  42. Peters G, van Groeningen C, Laurensse E, Lankelma J, Leyva A, Pinedo H. Uridine-induced hypothermia in mice and rats in relation to plasma and tissue levels of uridine and its metabolites. Cancer Chemother Pharmacol. 1987;20:101-8 pubmed
    ..Co-infusion of uridine with benzylacyclouridine, an inhibitor of uridine phosphorylase, partially prevented uridine-mediated hypothermia in mice...
  43. Lashkov A, Sotnichenko S, Prokofiev I, Gabdulkhakov A, Agapov I, Shtil A, et al. X-ray structure of Salmonella typhimurium uridine phosphorylase complexed with 5-fluorouracil and molecular modelling of the complex of 5-fluorouracil with uridine phosphorylase from Vibrio cholerae. Acta Crystallogr D Biol Crystallogr. 2012;68:968-74 pubmed publisher
    b>Uridine phosphorylase (UPh), which is a key enzyme in the reutilization pathway of pyrimidine nucleoside metabolism, is a validated target for the treatment of infectious diseases and cancer...
  44. Santos O, Perez L, Briggle T, Boothman D, Greer S. Radiation, pool size and incorporation studies in mice with 5-chloro-2'-deoxycytidine. Int J Radiat Oncol Biol Phys. 1990;19:357-65 pubmed
    ..5-Benzylacyclouridine (BAU), an inhibitor of uridine phosphorylase, was also utilized...
  45. Ali J, Tagoe D, Munday J, Donachie A, Morrison L, de Koning H. Pyrimidine biosynthesis is not an essential function for Trypanosoma brucei bloodstream forms. PLoS ONE. 2013;8:e58034 pubmed publisher
    ..African trypanosomes are capable of both pyrimidine biosynthesis and salvage of preformed pyrimidines from the host, but it is unknown whether either process is essential to the parasite...
  46. Walton L, Richards C, Elwell L. Nucleotide sequence of the Escherichia coli uridine phosphorylase (udp) gene. Nucleic Acids Res. 1989;17:6741 pubmed
  47. Xiong J, Zhang W, Su J, Shangguan J, Lin Y, Yang Y, et al. Improved synthesis of 2'-deoxyadenosine and 5-methyluridine by Escherichia coli using an auto-induction system. World J Microbiol Biotechnol. 2012;28:721-7 pubmed publisher
    ..E. coli BL21(DE3) strains overexpressing purine nucleoside phosphorylase (PNP), uridine phosphorylase (UP) and thymidine phosphorylase (TP) were constructed and cultured in auto-induction ZYM-Fe-5052 medium ..
  48. Rode W, Dabrowska M, Zielinski Z, Gołos B, Wranicz M, Felczak K, et al. Trichinella spiralis and Trichinella pseudospiralis: developmental patterns of enzymes involved in thymidylate biosynthesis and pyrimidine salvage. Parasitology. 2000;120 ( Pt 6):593-600 pubmed
    ..b>Uridine phosphorylase, another enzyme involved in 5-fluorouracil anabolism, was also present in T. pseudospiralis muscle larvae...
  49. el Kouni M, Goudgaon N, Rafeeq M, Al Safarjalani O, Schinazi R, Naguib F. 5-phenylthioacyclouridine: a potent and specific inhibitor of uridine phosphorylase. Biochem Pharmacol. 2000;60:851-6 pubmed
    ..was synthesized as a highly specific and potent inhibitor of uridine phosphorylase (UrdPase, EC 2.4.2.3)...
  50. Spoldi E, Ghisotti D, Cali S, Grisa M, Orsini G, Tonon G, et al. Recombinant bacterial cells as efficient biocatalysts for the production of nucleosides. Nucleosides Nucleotides Nucleic Acids. 2001;20:977-9 pubmed
  51. Rocchietti S, Ubiali D, Terreni M, Albertini A, Fernandez Lafuente R, Guisan J, et al. Immobilization and stabilization of recombinant multimeric uridine and purine nucleoside phosphorylases from Bacillus subtilis. Biomacromolecules. 2004;5:2195-200 pubmed
    ..Then, an extensive study of the UP (uridine phosphorylase) and PNP (purine nucleoside phosphorylase) immobilization and stabilization was carried out: optimal UP ..
  52. Zhu S, Ren L, Wang J, Zheng G, Tang P. Two-step efficient synthesis of 5-methyluridine via two thermostable nucleoside phosphorylase from Aeropyrum pernix. Bioorg Med Chem Lett. 2012;22:2102-4 pubmed publisher
    ..materials in the presence of highly thermostable recombinant purine nucleoside phosphorylase (PNP) and uridine phosphorylase (UP) obtained from hyperthermophilic aerobic crenarchaeon Aeropyrum pernix...
  53. Kubota T. Real-time RT-PCR (TaqMan) of tumor mRNA to predict sensitivity of specimens to 5-fluorouracil. Methods Mol Med. 2005;111:257-65 pubmed
    ..of thymidylate synthetase (TS), dihydropyrimidine dehydrogenase (DPD), thymidine phosphorylase (TP), uridine phosphorylase (UP), es-nucleoside transporter (NT), and E2F1 are detected by real-time reverse transcription polymerase ..