n acetylhexosaminyltransferases

Summary

Summary: Enzymes that catalyze the transfer of N-acetylhexosaminyl groups to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.

Top Publications

  1. Hildebrandt K, Anderson J. Biosynthetic elongation of isolated teichuronic acid polymers via glucosyl- and N-acetylmannosaminuronosyltransferases from solubilized cytoplasmic membrane fragments of Micrococcus luteus. J Bacteriol. 1990;172:5160-4 pubmed
    ..As shown by polyacrylamide gel electrophoresis, low-molecular-weight fractions of teichuronic acid are converted to higher-molecular-weight polymers by the addition of as many as 17 disaccharide repeat units...
  2. D Elia M, Henderson J, Beveridge T, Heinrichs D, Brown E. The N-acetylmannosamine transferase catalyzes the first committed step of teichoic acid assembly in Bacillus subtilis and Staphylococcus aureus. J Bacteriol. 2009;191:4030-4 pubmed publisher
  3. Xu L, Appell M, Kennedy S, Momany F, Price N. Conformational analysis of chirally deuterated tunicamycin as an active site probe of UDP-N-acetylhexosamine:polyprenol-P N-acetylhexosamine-1-P translocases. Biochemistry. 2004;43:13248-55 pubmed
    ..Our data support the hypothesis that tunicamycin is a structural analogue of the UDP-D-HexNAc substrate and is reversibly coordinated to the divalent metal cofactor in the D-HexNAc-1-P translocase active site. ..
  4. Wuyts W, Spieker N, van Roy N, De Boulle K, De Paepe A, Willems P, et al. Refined physical mapping and genomic structure of the EXTL1 gene. Cytogenet Cell Genet. 1999;86:267-70 pubmed
    ..In addition, we determined the genomic structure of the EXTL1 gene, revealing that the EXTL1 coding sequence spans 11 exons within a 50-kb region. ..
  5. Miyake K, Iijima S. Bacterial capsular polysaccharide and sugar transferases. Adv Biochem Eng Biotechnol. 2004;90:89-111 pubmed
  6. Murazumi N, Kumita K, Araki Y, Ito E. Partial purification and properties of UDP-N-acetylmannosamine:N-acetylglucosaminyl pyrophosphorylundecaprenol N-acetylmannosaminyltransferase from Bacillus subtilis. J Biochem. 1988;104:980-4 pubmed
    ..The enzyme was shown to be specific to UDP-ManNAc. The Km value for UDP-ManNAc was 4.4 microM, and the optimum pH was 7.3. The enzyme required 10 mM MgCl2, 0.3 M KCl, 25% glycerol, and 0.1% Nonidet P-40 to function at full activity. ..
  7. Viswanathan K, Narang S, Hinderlich S, Lee Y, Betenbaugh M. Engineering intracellular CMP-sialic acid metabolism into insect cells and methods to enhance its generation. Biochemistry. 2005;44:7526-34 pubmed
  8. Benie A, Blume A, Schmidt R, Reutter W, Hinderlich S, Peters T. Characterization of ligand binding to the bifunctional key enzyme in the sialic acid biosynthesis by NMR: II. Investigation of the ManNAc kinase functionality. J Biol Chem. 2004;279:55722-7 pubmed
    ..This provides a cornerstone for the rational design of drugs for novel therapeutic applications by altering the sialic acid decorations of cell walls. ..
  9. Mathysen D, Wuyts W, Bossuyt P, Wauters J, Van Hul W. Assignment of the mouse Extl1 gene to the distal part of chromosome 4 by in situ hybridization and radiation hybrid mapping. Cytogenet Cell Genet. 2001;92:162-3 pubmed

More Information

Publications18

  1. Stickens D, Brown D, Evans G. EXT genes are differentially expressed in bone and cartilage during mouse embryogenesis. Dev Dyn. 2000;218:452-64 pubmed
    ..We suggest a model for exostoses formation based on the involvement of EXT1 and EXT2 in the Indian hedgehog/parathyroid hormone-related peptide (PTHrP) signaling pathway, an important regulator of the chondrocyte maturation process...
  2. Kitagawa H, Egusa N, Tamura J, Kusche Gullberg M, Lindahl U, Sugahara K. rib-2, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes encodes a novel alpha1,4-N-acetylglucosaminyltransferase involved in the biosynthetic initiation and elongation of heparan sulfate. J Biol Chem. 2001;276:4834-8 pubmed
    ..These findings suggest that the biosynthetic mechanism of heparan sulfate in C. elegans is distinct from that reported for the mammalian system. ..
  3. Wuyts W, Van Hul W. Characterization and genomic localization of the mouse Extl2 gene. Cytogenet Cell Genet. 2000;89:185-8 pubmed
    ..By radiation hybrid analysis, Extl2 was mapped to mouse chromosome 3, in a region homologous to the human EXTL2 region on chromosome 1. ..
  4. Kurakake M, Goto T, Ashiki K, Suenaga Y, Komaki T. Synthesis of new glycosides by transglycosylation of N-acetylhexosaminidase from Serratia marcescens YS-1. J Agric Food Chem. 2003;51:1701-5 pubmed
    ..A dialcohol was used as an acceptor when the carbon number was more than 4 and a hydroxyl group existed on each of the two outside carbons. Sugar alcohols with hydroxyl groups in all carbon positions were not proper acceptors. ..
  5. Xu L, Xia J, Jiang H, Zhou J, Li H, Wang D, et al. Mutation analysis of hereditary multiple exostoses in the Chinese. Hum Genet. 1999;105:45-50 pubmed
    ..Our findings suggest a possibly different genetic spectrum of this disease in different populations...
  6. Kitagawa H, Shimakawa H, Sugahara K. The tumor suppressor EXT-like gene EXTL2 encodes an alpha1, 4-N-acetylhexosaminyltransferase that transfers N-acetylgalactosamine and N-acetylglucosamine to the common glycosaminoglycan-protein linkage region. The key enzyme for the chain initiation . J Biol Chem. 1999;274:13933-7 pubmed
  7. Schachter H. The joys of HexNAc. The synthesis and function of N- and O-glycan branches. Glycoconj J. 2000;17:465-83 pubmed
    ..7) The crystal structure of N-acetylglucosaminyltransferase I. (8) The discovery of the enzymes which synthesize O-glycan cores 1, 2, 3 and 4 and their elongation. ..
  8. Goddat J, Grey A, Hricovini M, Grushcow J, Carver J, Shah R. Synthesis of di- and tri-saccharides with intramolecular NH-glycosidic linkages: molecules with flexible and rigid glycosidic bonds for conformational studies. Carbohydr Res. 1994;252:159-70 pubmed
    ..The unusual intra NH-glycosyl character makes the linkage rigid, and therefore these compounds should not only be useful for NMR studies but also as substrates or inhibitors of GlcNAc-transferases. ..
  9. Barr K, Ward S, Meier Dieter U, Mayer H, Rick P. Characterization of an Escherichia coli rff mutant defective in transfer of N-acetylmannosaminuronic acid (ManNAcA) from UDP-ManNAcA to a lipid-linked intermediate involved in enterobacterial common antigen synthesis. J Bacteriol. 1988;170:228-33 pubmed
    ..Our results are consistent with the conclusion that the rff-726 mutation is located in the structural gene for the transferase that catalyzes the transfer of ManNAcA from UDP-ManNAcA to lipid I. ..