glycosyltransferases

Summary

Summary: Enzymes that catalyze the transfer of glycosyl groups to an acceptor. Most often another carbohydrate molecule acts as an acceptor, but inorganic phosphate can also act as an acceptor, such as in the case of PHOSPHORYLASES. Some of the enzymes in this group also catalyze hydrolysis, which can be regarded as transfer of a glycosyl group from the donor to water. Subclasses include the HEXOSYLTRANSFERASES; PENTOSYLTRANSFERASES; SIALYLTRANSFERASES; and those transferring other glycosyl groups. EC 2.4.

Top Publications

  1. Petit D, Teppa R, Petit J, Harduin Lepers A. A practical approach to reconstruct evolutionary history of animal sialyltransferases and gain insights into the sequence-function relationships of Golgi-glycosyltransferases. Methods Mol Biol. 2013;1022:73-97 pubmed publisher
  2. Wells L. The o-mannosylation pathway: glycosyltransferases and proteins implicated in congenital muscular dystrophy. J Biol Chem. 2013;288:6930-5 pubmed publisher
  3. Rennie E, Scheller H. Xylan biosynthesis. Curr Opin Biotechnol. 2014;26:100-7 pubmed publisher
    ..Recent research has identified several genes involved in xylan synthesis, some of which have already been used in engineering efforts to create plants that are better suited for biofuel production...
  4. Thiyagarajan N, Pham T, Stinson B, Sundriyal A, Tumbale P, Lizotte Waniewski M, et al. Structure of a metal-independent bacterial glycosyltransferase that catalyzes the synthesis of histo-blood group A antigen. Sci Rep. 2012;2:940 pubmed publisher
    ..HBGAs are also produced by a few prokaryotes where they are synthesized by glycosyltransferases (GTs) related to human HBGA synthases...
  5. Wilson D, Phamluong K, Lin W, Barck K, Carano R, Diehl L, et al. Chondroitin sulfate synthase 1 (Chsy1) is required for bone development and digit patterning. Dev Biol. 2012;363:413-25 pubmed publisher
    ..Our results place Chsy1 as an essential regulator of joint patterning and provide a mouse model of human brachydactylies caused by mutations in CHSY1. ..
  6. Breton C, Fournel Gigleux S, Palcic M. Recent structures, evolution and mechanisms of glycosyltransferases. Curr Opin Struct Biol. 2012;22:540-9 pubmed publisher
    Cellular glycome assembly requires the coordinated action of a large number of glycosyltransferases that catalyse the transfer of a sugar residue from a donor to specific acceptor molecules...
  7. Velasquez S, Ricardi M, Dorosz J, Fernandez P, Nadra A, Pol Fachin L, et al. O-glycosylated cell wall proteins are essential in root hair growth. Science. 2011;332:1401-3 pubmed publisher
    ..Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana. ..
  8. Ruan X, Loyola D, Marolda C, Perez Donoso J, Valvano M. The WaaL O-antigen lipopolysaccharide ligase has features in common with metal ion-independent inverting glycosyltransferases. Glycobiology. 2012;22:288-99 pubmed publisher
    ..Together, our biochemical and in silico data argue that WaaL proteins use a common reaction mechanism and share features of metal ion-independent inverting glycosyltransferases.
  9. Yu R, Tsai Y, Ariga T. Functional roles of gangliosides in neurodevelopment: an overview of recent advances. Neurochem Res. 2012;37:1230-44 pubmed publisher
    ..In this review, we focus on recent advances on metabolic and functional studies of gangliosides in normal brain development and in certain neurological disorders. ..

Scientific Experts

More Information

Publications62

  1. Desmet T, Soetaert W, Bojarová P, Kren V, Dijkhuizen L, Eastwick Field V, et al. Enzymatic glycosylation of small molecules: challenging substrates require tailored catalysts. Chemistry. 2012;18:10786-801 pubmed publisher
    ..Progress in the field of enzyme engineering and screening are expected to result in new applications of biocatalytic glycosylation reactions in various industrial sectors. ..
  2. Harholt J, Sørensen I, Fangel J, Roberts A, Willats W, Scheller H, et al. The glycosyltransferase repertoire of the spikemoss Selaginella moellendorffii and a comparative study of its cell wall. PLoS ONE. 2012;7:e35846 pubmed publisher
    ..b>Glycosyltransferases (GTs) are involved in many aspects of a plant life, including cell wall biosynthesis, protein glycosylation,..
  3. Gahlan P, Singh H, Shankar R, Sharma N, Kumari A, Chawla V, et al. De novo sequencing and characterization of Picrorhiza kurrooa transcriptome at two temperatures showed major transcriptome adjustments. BMC Genomics. 2012;13:126 pubmed publisher
    ..In order to gain insight into temperature mediated molecular changes, high throughput de novo transcriptome sequencing and analyses were carried out at 15 °C and 25 °C, the temperatures known to modulate picrosides content...
  4. Gómez H, Polyak I, Thiel W, Lluch J, Masgrau L. Retaining glycosyltransferase mechanism studied by QM/MM methods: lipopolysaccharyl-?-1,4-galactosyltransferase C transfers ?-galactose via an oxocarbenium ion-like transition state. J Am Chem Soc. 2012;134:4743-52 pubmed publisher
    b>Glycosyltransferases (GTs) catalyze the highly specific biosynthesis of glycosidic bonds and, as such, are important both as drug targets and for biotechnological purposes...
  5. Banerjee D. N-glycans in cell survival and death: cross-talk between glycosyltransferases. Biochim Biophys Acta. 2012;1820:1338-46 pubmed publisher
    ..It is expected that there is a great deal of cross-talk between the participating glycosyltransferases and any missed step would express defective N-glycans that could have fatal consequences...
  6. Owatworakit A, Townsend B, Louveau T, Jenner H, Rejzek M, Hughes R, et al. Glycosyltransferases from oat (Avena) implicated in the acylation of avenacins. J Biol Chem. 2013;288:3696-704 pubmed publisher
    ..Many of these compounds are glycosylated by family 1 glycosyltransferases (GTs). Oats (Avena spp...
  7. James D, Gupta K, Hauser J, Yother J. Biochemical activities of Streptococcus pneumoniae serotype 2 capsular glycosyltransferases and significance of suppressor mutations affecting the initiating glycosyltransferase Cps2E. J Bacteriol. 2013;195:5469-78 pubmed publisher
    ..In this study, we examined the functions of three capsular glycosyltransferases (Cps2F, Cps2G, and Cps2I) involved in serotype 2 CPS synthesis, whose deletions select for secondary ..
  8. Yonekura Sakakibara K, Fukushima A, Nakabayashi R, Hanada K, Matsuda F, Sugawara S, et al. Two glycosyltransferases involved in anthocyanin modification delineated by transcriptome independent component analysis in Arabidopsis thaliana. Plant J. 2012;69:154-67 pubmed publisher
    ..Two glycosyltransferases, UGT79B1 and UGT84A2 were found to cluster with anthocyanin biosynthetic genes...
  9. Brown S, Xia G, Luhachack L, Campbell J, Meredith T, Chen C, et al. Methicillin resistance in Staphylococcus aureus requires glycosylated wall teichoic acids. Proc Natl Acad Sci U S A. 2012;109:18909-14 pubmed publisher
    ..These data suggest ?-O-GlcNAcylated WTAs scaffold factors required for MRSA resistance. The ?-O-GlcNAc transferase identified here, TarS, is a unique target for antimicrobials that sensitize MRSA to ?-lactams...
  10. Kovinich N, Saleem A, Arnason J, Miki B. Combined analysis of transcriptome and metabolite data reveals extensive differences between black and brown nearly-isogenic soybean (Glycine max) seed coats enabling the identification of pigment isogenes. BMC Genomics. 2011;12:381 pubmed publisher
    ..Duplicate gene copies for several enzymes were overexpressed in the black (iRT) seed coat suggesting more than one isogene may have to be silenced to engineer seed coat color using RNA interference...
  11. Børud B, Viburiene R, Hartley M, Paulsen B, Egge Jacobsen W, Imperiali B, et al. Genetic and molecular analyses reveal an evolutionary trajectory for glycan synthesis in a bacterial protein glycosylation system. Proc Natl Acad Sci U S A. 2011;108:9643-8 pubmed publisher
    ..we identified a metabolic conflict related to competition for a shared substrate between the opposing glycosyltransferases PglA and PglH...
  12. Chaturvedi P, Mishra M, Akhtar N, Gupta P, Mishra P, Tuli R. Sterol glycosyltransferases-identification of members of gene family and their role in stress in Withania somnifera. Mol Biol Rep. 2012;39:9755-64 pubmed publisher
    Sterol glycosyltransferases (SGTs) catalyze the transfer of sugar molecules to diverse sterol molecules, leading to a change in their participation in cellular metabolism...
  13. Mancilla M, Marin C, Blasco J, Zárraga A, López Goñi I, Moriyon I. Spontaneous excision of the O-polysaccharide wbkA glycosyltranferase gene is a cause of dissociation of smooth to rough Brucella colonies. J Bacteriol. 2012;194:1860-7 pubmed publisher
    ..This ISBm1-mediated wbkA excision and the different %GC levels of the excised fragment and of other wbk genes suggest that the Brucella wbk locus is the result of at least two horizontal acquisition events...
  14. Anders N, Wilkinson M, Lovegrove A, Freeman J, Tryfona T, Pellny T, et al. Glycosyl transferases in family 61 mediate arabinofuranosyl transfer onto xylan in grasses. Proc Natl Acad Sci U S A. 2012;109:989-93 pubmed publisher
    ..Thus, GT61 proteins play a key role in arabinoxylan biosynthesis and therefore in the evolutionary divergence of grass cell walls...
  15. Nikolovski N, Rubtsov D, Segura M, Miles G, Stevens T, Dunkley T, et al. Putative glycosyltransferases and other plant Golgi apparatus proteins are revealed by LOPIT proteomics. Plant Physiol. 2012;160:1037-51 pubmed publisher
    ..Other newly identified proteins are likely to be involved in the transport and interconversion of nucleotide sugar substrates as well as polysaccharide and protein modification...
  16. Lee C, Teng Q, Zhong R, Ye Z. Arabidopsis GUX proteins are glucuronyltransferases responsible for the addition of glucuronic acid side chains onto xylan. Plant Cell Physiol. 2012;53:1204-16 pubmed publisher
    ..Together, our results provide biochemical and genetic evidence that GUX1/2/3 are GlcATs responsible for the GlcA substitution of xylan, which is essential for normal secondary wall deposition and plant development. ..
  17. Schweiger W, Pasquet J, Nussbaumer T, Paris M, Wiesenberger G, Macadré C, et al. Functional characterization of two clusters of Brachypodium distachyon UDP-glycosyltransferases encoding putative deoxynivalenol detoxification genes. Mol Plant Microbe Interact. 2013;26:781-92 pubmed publisher
    Plant small-molecule UDP-glycosyltransferases (UGT) glycosylate a vast number of endogenous substances but also act in detoxification of metabolites produced by plant-pathogenic microorganisms...
  18. Filipek Gorniok B, Holmborn K, Haitina T, Habicher J, Oliveira M, Hellgren C, et al. Expression of chondroitin/dermatan sulfate glycosyltransferases during early zebrafish development. Dev Dyn. 2013;242:964-75 pubmed publisher
    ..is represented by two copies in the zebrafish genome, chpfa and chpfb, while the other five human CS/DS glycosyltransferases csgalnact1, csgalnact2, chpf2, chsy1, and chsy3 all have single zebrafish orthologues...
  19. Oliver M, van der Linden M, Küntzel S, Saad J, Nahm M. Discovery of Streptococcus pneumoniae serotype 6 variants with glycosyltransferases synthesizing two differing repeating units. J Biol Chem. 2013;288:25976-85 pubmed publisher
    ..Single amino acid changes in cps genes encoding glycosyltransferases can alter substrate specificities, permit biosynthesis of heterogeneous capsule repeating units, and result ..
  20. Wang Z, Chinoy Z, Ambre S, Peng W, McBride R, de Vries R, et al. A general strategy for the chemoenzymatic synthesis of asymmetrically branched N-glycans. Science. 2013;341:379-83 pubmed publisher
    ..were selected so that the antenna of the resulting deprotected compounds could be selectively extended by glycosyltransferases to give libraries of asymmetrical multi-antennary glycans...
  21. Cai C, Edgar K, Liu J, Linhardt R. Preparation and application of a 'clickable' acceptor for enzymatic synthesis of heparin oligosaccharides. Carbohydr Res. 2013;372:30-4 pubmed publisher
  22. Bromley J, Busse Wicher M, Tryfona T, Mortimer J, Zhang Z, Brown D, et al. GUX1 and GUX2 glucuronyltransferases decorate distinct domains of glucuronoxylan with different substitution patterns. Plant J. 2013;74:423-34 pubmed publisher
    ..These findings have substantial implications for our understanding of xylan synthesis and structure, and for models of the molecular architecture of the lignocellulosic matrix of plant cell walls. ..
  23. Kulkarni A, Peña M, Avci U, Mazumder K, Urbanowicz B, Pattathil S, et al. The ability of land plants to synthesize glucuronoxylans predates the evolution of tracheophytes. Glycobiology. 2012;22:439-51 pubmed publisher
    ..patens genome contains genes with high sequence similarity to Arabidopsis CAZy family GT8, GT43 and GT47 glycosyltransferases that are likely involved in xylan synthesis...
  24. Suliman M, Chateigner Boutin A, Francin Allami M, Partier A, Bouchet B, Salse J, et al. Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm. J Proteomics. 2013;78:508-21 pubmed publisher
    ..We report the identification of 1135 proteins among which 64 glycosyltransferases distributed in 17 families. Their potential function in cell wall synthesis is discussed...
  25. Brown C, Leijon F, Bulone V. Radiometric and spectrophotometric in vitro assays of glycosyltransferases involved in plant cell wall carbohydrate biosynthesis. Nat Protoc. 2012;7:1634-50 pubmed publisher
    Most of the glycosyltransferases (GTs) that catalyze the formation of plant cell wall carbohydrates remain to be biochemically characterized. This can be achieved only if specific assays are available for these enzymes...
  26. Kawai F, Grass S, Kim Y, Choi K, St Geme J, Yeo H. Structural insights into the glycosyltransferase activity of the Actinobacillus pleuropneumoniae HMW1C-like protein. J Biol Chem. 2011;286:38546-57 pubmed publisher
  27. ORR S, Le D, Long J, Sobieszczuk P, Ma B, Tian H, et al. A phenotype survey of 36 mutant mouse strains with gene-targeted defects in glycosyltransferases or glycan-binding proteins. Glycobiology. 2013;23:363-80 pubmed publisher
  28. Chan J, Tang A, Bennet A. A stepwise solvent-promoted SNi reaction of ?-D-glucopyranosyl fluoride: mechanistic implications for retaining glycosyltransferases. J Am Chem Soc. 2012;134:1212-20 pubmed publisher
    ..The implications that this model reaction have for the mode of action of retaining glycosyltransferases are discussed.
  29. Atmodjo M, Hao Z, Mohnen D. Evolving views of pectin biosynthesis. Annu Rev Plant Biol. 2013;64:747-79 pubmed publisher
    ..The functional confirmation of four types of pectin biosynthetic glycosyltransferases, the identification of multiple putative pectin glycosyl- and methyltransferases, and the characteristics ..
  30. Lee C, Teng Q, Zhong R, Ye Z. Molecular dissection of xylan biosynthesis during wood formation in poplar. Mol Plant. 2011;4:730-47 pubmed publisher
    ..In this report, we investigated roles of poplar families GT43 and GT8 glycosyltransferases in xylan biosynthesis during wood formation...
  31. Wandall H, Rumjantseva V, Sørensen A, Patel Hett S, Josefsson E, Bennett E, et al. The origin and function of platelet glycosyltransferases. Blood. 2012;120:626-35 pubmed publisher
    ..Immunocytology and flow cytometry results indicated that megakaryocytes package these Golgi-derived glycosyltransferases into vesicles that are sent via proplatelets to nascent platelets, where they accumulate...
  32. Nagatoshi M, Terasaka K, Nagatsu A, Mizukami H. Iridoid-specific glucosyltransferase from Gardenia jasminoides. J Biol Chem. 2011;286:32866-74 pubmed publisher
    ..The mRNA level of UGT85A24 overlaps with the marked increase in genipin glucosylation activity in the methyl jasmonate-treated cell cultures of G. jasminoides and is related to iridoid accumulation in G. jasminoides fruits...
  33. Leemhuis H, Pijning T, Dobruchowska J, van Leeuwen S, Kralj S, Dijkstra B, et al. Glucansucrases: three-dimensional structures, reactions, mechanism, ?-glucan analysis and their implications in biotechnology and food applications. J Biotechnol. 2013;163:250-72 pubmed publisher
    ..Finally we discuss the (potential) applications of ?-glucans produced by lactic acid bacteria in food and health related industries. ..
  34. Bhat W, Dhar N, Razdan S, Rana S, Mehra R, Nargotra A, et al. Molecular characterization of UGT94F2 and UGT86C4, two glycosyltransferases from Picrorhiza kurrooa: comparative structural insight and evaluation of substrate recognition. PLoS ONE. 2013;8:e73804 pubmed publisher
    Uridine diphosphate glycosyltransferases (UGTs) are pivotal in the process of glycosylation for decorating natural products with sugars...
  35. Gomez Casati D, Martin M, Busi M. Polysaccharide-synthesizing glycosyltransferases and carbohydrate binding modules: the case of starch synthase III. Protein Pept Lett. 2013;20:856-63 pubmed
    b>Glycosyltransferases (GTs) are a ubiquitous group of enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds...
  36. Rennie E, Hansen S, Baidoo E, Hadi M, Keasling J, Scheller H. Three members of the Arabidopsis glycosyltransferase family 8 are xylan glucuronosyltransferases. Plant Physiol. 2012;159:1408-17 pubmed publisher
    ..GUX1 has characteristics typical of Golgi-localized glycosyltransferases and a K(m) for UDP-GlcA of 165 ?m...
  37. von Saint Paul V, Zhang W, Kanawati B, Geist B, Faus Kessler T, Schmitt Kopplin P, et al. The Arabidopsis glucosyltransferase UGT76B1 conjugates isoleucic acid and modulates plant defense and senescence. Plant Cell. 2011;23:4124-45 pubmed publisher
    ..Exogenously applied isoleucic acid increased resistance against P. syringae infection. These findings indicate a novel link between amino acid-related molecules and plant defense that is mediated by small-molecule glucosylation. ..
  38. Harholt J, Jensen J, Verhertbruggen Y, Søgaard C, Bernard S, Nafisi M, et al. ARAD proteins associated with pectic Arabinan biosynthesis form complexes when transiently overexpressed in planta. Planta. 2012;236:115-28 pubmed publisher
    ..Altogether, these data show that ARAD2 is associated with arabinan biosynthesis, not redundant with ARAD1, and that the two glycosyltransferases may function in complexes held together by disulfide bridges.
  39. Xu Y, Masuko S, Takieddin M, Xu H, Liu R, Jing J, et al. Chemoenzymatic synthesis of homogeneous ultralow molecular weight heparins. Science. 2011;334:498-501 pubmed publisher
    ..The chemoenzymatic approach is scalable and shows promise for a more efficient route to synthesize this important class of medicinal agent. ..
  40. Charbonneau M, Côté J, Haurat M, Reiz B, Crépin S, Berthiaume F, et al. A structural motif is the recognition site for a new family of bacterial protein O-glycosyltransferases. Mol Microbiol. 2012;83:894-907 pubmed publisher
    ..Overall, our findings suggest that Aah represents the prototype of a new large family of bacterial protein O-glycosyltransferases that modify various substrates recognized through a structural motif.
  41. Brown D, Zhang Z, Stephens E, Dupree P, Turner S. Characterization of IRX10 and IRX10-like reveals an essential role in glucuronoxylan biosynthesis in Arabidopsis. Plant J. 2009;57:732-46 pubmed publisher
    Xylan, the major hemicellulosic polysaccharide in Arabidopsis secondary cell walls, requires a number of glycosyltransferases (GT) to catalyse formation of the various glycosidic linkages found in the polymer...
  42. Xu K, Nieuwenhuis E, Cohen B, Wang W, Canty A, Danska J, et al. Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis. Am J Physiol Lung Cell Mol Physiol. 2010;298:L45-56 pubmed publisher
    ..In sum, these results demonstrate that Lfng functions to enhance Notch signaling in myofibroblast precursor cells and thereby to coordinate differentiation and mobilization of myofibroblasts required for alveolar septation. ..
  43. Schoberer J, Strasser R. Sub-compartmental organization of Golgi-resident N-glycan processing enzymes in plants. Mol Plant. 2011;4:220-8 pubmed publisher
    ..It is widely accepted that the glycosidases and glycosyltransferases involved in N-glycan processing are found concentrated within the Golgi stack where they provide their ..
  44. Lorenc Kukuła K, Zuk M, Kulma A, Czemplik M, Kostyn K, Skala J, et al. Engineering Flax with the GT Family 1 Solanum sogarandinum Glycosyltransferase SsGT1 Confers Increased Resistance to Fusarium Infection. J Agric Food Chem. 2009;57:6698-705 pubmed publisher
    ..It is thus suggested that introduction of SsGT1 is sufficient for engineering altered pathogen resistance in flax...
  45. Zhou Y, Li S, Qian Q, Zeng D, Zhang M, Guo L, et al. BC10, a DUF266-containing and Golgi-located type II membrane protein, is required for cell-wall biosynthesis in rice (Oryza sativa L.). Plant J. 2009;57:446-62 pubmed publisher
    b>Glycosyltransferases (GTs) are one of the largest enzyme groups required for the synthesis of complex wall polysaccharides and glycoproteins in plants...
  46. Park S, Park H, Sohng J, Lee H, Liou K, Yoon Y, et al. Expanding substrate specificity of GT-B fold glycosyltransferase via domain swapping and high-throughput screening. Biotechnol Bioeng. 2009;102:988-94 pubmed publisher
    b>Glycosyltransferases (GTs) are crucial enzymes in the biosynthesis and diversification of therapeutically important natural products, and the majority of them belong to the GT-B superfamily, which is composed of separate N- and C-domains ..
  47. Thibodeaux C, MELANCON C, Liu H. Natural-product sugar biosynthesis and enzymatic glycodiversification. Angew Chem Int Ed Engl. 2008;47:9814-59 pubmed publisher
    ..Remarkably, many of these sugar biosynthetic enzymes and glycosyltransferases also exhibit relaxed substrate specificity...
  48. Lee Y, Kim A, Park M, Kim H, Lee K, Park Y. Molecular cloning of cyanobacterial pteridine glycosyltransferases that catalyze the transfer of either glucose or xylose to tetrahydrobiopterin. Appl Environ Microbiol. 2010;76:7658-61 pubmed publisher
    Here, we report cloning of cyanobacterial genes encoding pteridine glycosyltransferases that catalyze glucosyl or xylosyl transfer from UDP-sugars to tetrahydrobiopterin...
  49. Olsen S, Boyle S, Schurig G, Sriranganathan N. Immune responses and protection against experimental challenge after vaccination of bison with Brucella abortus strain RB51 or RB51 overexpressing superoxide dismutase and glycosyltransferase genes. Clin Vaccine Immunol. 2009;16:535-40 pubmed publisher
    ..Our data also suggest that the RB51 vaccine is a currently available management tool that could be utilized to help reduce brucellosis in free-ranging bison...
  50. Hansen E, Møller B, Kock G, Bünner C, Kristensen C, Jensen O, et al. De novo biosynthesis of vanillin in fission yeast (Schizosaccharomyces pombe) and baker's yeast (Saccharomyces cerevisiae). Appl Environ Microbiol. 2009;75:2765-74 pubmed publisher
    ..S. pombe yeast has not previously been metabolically engineered to produce any valuable, industrially scalable, white biotech commodity...
  51. Godfrey C, Foley A, Clement E, Muntoni F. Dystroglycanopathies: coming into focus. Curr Opin Genet Dev. 2011;21:278-85 pubmed publisher
    ..Defects in a total of eight putative and demonstrated glycosyltransferases or accessory proteins of glycosyltransferases have been shown to cause a dystroglycanopathy phenotype...
  52. Petersen B, Egelund J, Damager I, Faber K, Jensen J, Yang Z, et al. Assay and heterologous expression in Pichia pastoris of plant cell wall type-II membrane anchored glycosyltransferases. Glycoconj J. 2009;26:1235-46 pubmed publisher
    ..In summary, the data presented suggest that Pichia is an attractive host candidate for expression of plant glycosyltransferases.
  53. Kong Y, Zhou G, Avci U, Gu X, Jones C, Yin Y, et al. Two poplar glycosyltransferase genes, PdGATL1.1 and PdGATL1.2, are functional orthologs to PARVUS/AtGATL1 in Arabidopsis. Mol Plant. 2009;2:1040-50 pubmed publisher
    ..Our data indicate that PdGATL1.1 and PdGATL1.2 are functional orthologs of PARVUS/GATL1 and can play a role in xylan synthesis, but may also have role(s) in the synthesis of other wall polymers. ..