transketolase

Summary

Summary: An enzyme of the transferase class that catalyzes the conversion of sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate to D-ribose 5-phosphate and D-xylulose 5-phosphate in the PENTOSE PHOSPHATE PATHWAY. (Dorland, 27th ed) EC 2.2.1.1.

Top Publications

  1. Esakova O, Meshalkina L, Golbik R, Hübner G, Kochetov G. Donor substrate regulation of transketolase. Eur J Biochem. 2004;271:4189-94 pubmed
    ..but not the acceptor substrates, enhance the affinity of the coenzyme either to only one active center of transketolase or to both active centers, but to different degrees in each, resulting in a negative cooperativity for ..
  2. Domain F, Bina X, Levy S. Transketolase A, an enzyme in central metabolism, derepresses the marRAB multiple antibiotic resistance operon of Escherichia coli by interaction with MarR. Mol Microbiol. 2007;66:383-94 pubmed
    ..coli DNA fragments in the bacterial adenylate cyclase two-hybrid system, we found that transketolase A (TktA) interacts with MarR. Purified (6H)-TktA immobilized on NiNTA resin-bound MarR...
  3. Rais B, Comin B, Puigjaner J, Brandes J, Creppy E, Saboureau D, et al. Oxythiamine and dehydroepiandrosterone induce a G1 phase cycle arrest in Ehrlich's tumor cells through inhibition of the pentose cycle. FEBS Lett. 1999;456:113-8 pubmed
    b>Transketolase (TK) reactions play a crucial role in tumor cell nucleic acid ribose synthesis utilizing glucose carbons, yet, current cancer treatments do not target this central pathway...
  4. Oh S, Witek R, Bae S, Darwiche H, Jung Y, Pi L, et al. Detection of transketolase in bone marrow-derived insulin-producing cells: benfotiamine enhances insulin synthesis and glucose metabolism. Stem Cells Dev. 2009;18:37-46 pubmed publisher
    ..Here, we demonstrate the detection of the pentose phosphate pathway enzyme, transketolase (TK), in BM-derived IPCs cultured under high-glucose conditions...
  5. Völker H, Scheich M, Schmausser B, Kammerer U, Eck M. Overexpression of transketolase TKTL1 is associated with shorter survival in laryngeal squamous cell carcinomas. Eur Arch Otorhinolaryngol. 2007;264:1431-6 pubmed
    ..One of the key enzymes is transketolase. Transketolase, transketolase-like-1 (TKTL1), and transketolase-like-2 are known...
  6. Hu L, Yang J, Zhang D, Zhang S, Wang L, Cai P, et al. The TKTL1 gene influences total transketolase activity and cell proliferation in human colon cancer LoVo cells. Anticancer Drugs. 2007;18:427-33 pubmed
    A plasmid carrying DNA to be transcribed into a small interfering RNA against transketolase-like-1 mRNA was constructed and transfected into a human colon cancer cell line...
  7. Flores N, Xiao J, Berry A, Bolivar F, Valle F. Pathway engineering for the production of aromatic compounds in Escherichia coli. Nat Biotechnol. 1996;14:620-3 pubmed
    ..This increased carbon commitment to the aromatic pathway was enhanced still further upon amplification of the E. coli tktA gene that encodes for a transketolase involved in the biosynthesis of E4P.
  8. Ingram C, Bommer M, Smith M, Dalby P, Ward J, Hailes H, et al. One-pot synthesis of amino-alcohols using a de-novo transketolase and beta-alanine: pyruvate transaminase pathway in Escherichia coli. Biotechnol Bioeng. 2007;96:559-69 pubmed
    ..The enzymes transketolase (TK) and transaminase (TAm) respectively catalyze asymmetric carbon--carbon bond formation and amine group ..
  9. Stoffel S, Alibu V, Hubert J, Ebikeme C, Portais J, Bringaud F, et al. Transketolase in Trypanosoma brucei. Mol Biochem Parasitol. 2011;179:1-7 pubmed publisher
    A single copy gene, encoding a protein highly similar to transketolase from other systems, was identified in the Trypanosoma brucei genome. The gene was expressed in E...

More Information

Publications61

  1. Coy J, Dubel S, Kioschis P, Thomas K, Micklem G, Delius H, et al. Molecular cloning of tissue-specific transcripts of a transketolase-related gene: implications for the evolution of new vertebrate genes. Genomics. 1996;32:309-16 pubmed
    As part of a systematic search for differentially expressed genes, we have isolated a novel transketolase-related gene (TKR) (HGMW-approved symbol TKT), located between the green color vision pigment gene (GCP) and the ABP-280 filamin ..
  2. Schenk G, Layfield R, Candy J, Duggleby R, Nixon P. Molecular evolutionary analysis of the thiamine-diphosphate-dependent enzyme, transketolase. J Mol Evol. 1997;44:552-72 pubmed
    Members of the transketolase group of thiamine-diphosphate-dependent enzymes from 17 different organisms including mammals, yeast, bacteria, and plants have been used for phylogenetic reconstruction...
  3. Lee J, Cheong D, Kim G. A novel assay system for the measurement of transketolase activity using xylulokinase from Saccharomyces cerevisiae. Biotechnol Lett. 2008;30:899-904 pubmed
    The conventional method of transketolase (TKT) activity assay uses ribose 5-phosphate and xylulose 5-phosphate as substrates...
  4. Schenk G, Duggleby R, Nixon P. Heterologous expression of human transketolase. Int J Biochem Cell Biol. 1998;30:369-78 pubmed
    b>Transketolase belongs to the family of thiamin diphosphate dependent enzymes...
  5. Park Y, Kim S, Park K, Lee K, Seo J. Fed-batch production of D-ribose from sugar mixtures by transketolase-deficient Bacillus subtilis SPK1. Appl Microbiol Biotechnol. 2004;66:297-302 pubmed
    ..A high D-ribose-producing Bacillus subtilis SPK1 strain was constructed by the chemical mutation of the transketolase-deficient strain, B. subtilis JY1. Batch fermentation of B...
  6. Jung E, Itokawa Y, Nishino K. Effect of chronic alcohol administration on transketolase in the brain and the liver of rats. Am J Clin Nutr. 1991;53:100-5 pubmed
    ..thiamin-deficiency-related diseases, especially Wernicke-Korsakoff syndrome, we studied the relationship among transketolase activity, transketolase concentration, and thiamin phosphate esters in rats chronically fed alcohol...
  7. Coy J, Dressler D, Wilde J, Schubert P. Mutations in the transketolase-like gene TKTL1: clinical implications for neurodegenerative diseases, diabetes and cancer. Clin Lab. 2005;51:257-73 pubmed
    b>Transketolase proteins or transketolase enzyme activities have been related to neurodegenerative diseases, diabetes, and cancer...
  8. Zhao J, Zhong C. A review on research progress of transketolase. Neurosci Bull. 2009;25:94-9 pubmed publisher
    b>Transketolase (TK), a thiamine diphosphate (ThDP)-dependent enzyme, catalyzes several key reactions of non-oxidative branch of pentose phosphate pathway...
  9. Mayer A, von Wallbrunn A, Vaupel P. Glucose metabolism of malignant cells is not regulated by transketolase-like (TKTL)-1. Int J Oncol. 2010;37:265-71 pubmed
    An isoenzyme of transketolase, transketolase-like (TKTL)-1, has been hypothesized to play a pivotal role in the pathophysiology of malignant tumors...
  10. Grimm M, Hoefert S, Luz O, Reinert S, Polligkeit J. Transketolase-like protein 1 expression in recurrent oral squamous cell carcinoma after curative resection: a case report. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013;116:e173-8 pubmed publisher
    ..b>Transketolase-like protein 1 (TKTL1), which has an important role in controlling the nonoxidative pentose-phosphate pathway, ..
  11. Teige M, Melzer M, Süss K. Purification, properties and in situ localization of the amphibolic enzymes D-ribulose 5-phosphate 3-epimerase and transketolase from spinach chloroplasts. Eur J Biochem. 1998;252:237-44 pubmed
    The amphibolic enzymes D-ribulose 5-phosphate 3-epimerase and transketolase have been purified from stroma extracts of spinach chloroplasts using ammonium sulfate fractionation and FPLC...
  12. Benov L, Fridovich I. Why superoxide imposes an aromatic amino acid auxotrophy on Escherichia coli. The transketolase connection. J Biol Chem. 1999;274:4202-6 pubmed
    ..It does so by oxidizing the 1, 2-dihydroxyethyl thiamine pyrophosphate intermediate of transketolase and inactivating this enzyme.
  13. Braun L, Puskas F, Csala M, Meszaros G, Mandl J, Banhegyi G. Ascorbate as a substrate for glycolysis or gluconeogenesis: evidence for an interorgan ascorbate cycle. Free Radic Biol Med. 1997;23:804-8 pubmed
    ..The oxidative agent menadione stimulated, while the transketolase inhibitor oxythiamine inhibited, the metabolism of dehydroascorbate in each of these three cell types...
  14. Simon G, Bouzon M, Charmantray F, Helaine V, Légeret B, Marliere P, et al. Amino acid precursors for the detection of transketolase activity in Escherichia coli auxotrophs. Bioorg Med Chem Lett. 2009;19:3767-70 pubmed publisher
    Probes were developed for the in vivo detection of transketolase activity by the use of a complementation assay in Escherichia coli auxotrophs They combine the d-threo ketose moiety recognised by transketolase and the side chain of ..
  15. Golbik R, Meshalkina L, Sandalova T, Tittmann K, Fiedler E, Neef H, et al. Effect of coenzyme modification on the structural and catalytic properties of wild-type transketolase and of the variant E418A from Saccharomyces cerevisiae. FEBS J. 2005;272:1326-42 pubmed
    b>Transketolase from baker's yeast is a thiamin diphosphate-dependent enzyme in sugar metabolism that reconstitutes with various analogues of the coenzyme...
  16. Boren J, Montoya A, de Atauri P, Comin Anduix B, Cortes A, Centelles J, et al. Metabolic control analysis aimed at the ribose synthesis pathways of tumor cells: a new strategy for antitumor drug development. Mol Biol Rep. 2002;29:7-12 pubmed
    ..41 for G6PDH on tumor cell growth was computed. This value is approximately the half of the transketolase control coefficient value of 0.9 previously reported...
  17. Rolland A, Lavigne R, Dauly C, Calvel P, Kervarrec C, Freour T, et al. Identification of genital tract markers in the human seminal plasma using an integrative genomics approach. Hum Reprod. 2013;28:199-209 pubmed publisher
  18. Schaaff Gerstenschläger I, Mannhaupt G, Vetter I, Zimmermann F, Feldmann H. TKL2, a second transketolase gene of Saccharomyces cerevisiae. Cloning, sequence and deletion analysis of the gene. Eur J Biochem. 1993;217:487-92 pubmed
    b>Transketolase activity is indispensable for the generation of erythrose 4-phosphate and therefore necessary for the biosynthesis of the aromatic amino acids...
  19. Ikeda M, Katsumata R. Hyperproduction of tryptophan by Corynebacterium glutamicum with the modified pentose phosphate pathway. Appl Environ Microbiol. 1999;65:2497-502 pubmed
    ..transition by increasing the supply of erythrose 4-phosphate, a direct precursor of aromatic biosynthesis, the transketolase gene of C...
  20. Schneider G, Lindqvist Y. Crystallography and mutagenesis of transketolase: mechanistic implications for enzymatic thiamin catalysis. Biochim Biophys Acta. 1998;1385:387-98 pubmed
    The ThDP dependent enzyme transketolase is a convenient model system to study enzymatic thiamin catalysis...
  21. Sprenger G, Schörken U, Sprenger G, Sahm H. Transketolase A of Escherichia coli K12. Purification and properties of the enzyme from recombinant strains. Eur J Biochem. 1995;230:525-32 pubmed
    b>Transketolase A was purified to apparent homogeneity from recombinant Escherichia coli K12 cells carrying the homologous cloned tktA gene on a pUC19-derived plasmid...
  22. Kayser G, Kassem A, Sienel W, Schulte Uentrop L, Mattern D, Aumann K, et al. Lactate-dehydrogenase 5 is overexpressed in non-small cell lung cancer and correlates with the expression of the transketolase-like protein 1. Diagn Pathol. 2010;5:22 pubmed publisher
    ..In addition, the results of the previously tested transketolase like 1 protein (TKTL1) expression were correlated to LDH5 expression. 89...
  23. Abedinia M, Layfield R, Jones S, Nixon P, Mattick J. Nucleotide and predicted amino acid sequence of a cDNA clone encoding part of human transketolase. Biochem Biophys Res Commun. 1992;183:1159-66 pubmed
    b>Transketolase is a key enzyme in the pentose-phosphate pathway which has been implicated in the latent human genetic disease, Wernicke-Korsakoff syndrome...
  24. Smith I, Glazer C, Mithani S, Ochs M, Sun W, Bhan S, et al. Coordinated activation of candidate proto-oncogenes and cancer testes antigens via promoter demethylation in head and neck cancer and lung cancer. PLoS ONE. 2009;4:e4961 pubmed publisher
  25. Aucamp J, Martinez Torres R, Hibbert E, Dalby P. A microplate-based evaluation of complex denaturation pathways: structural stability of Escherichia coli transketolase. Biotechnol Bioeng. 2008;99:1303-10 pubmed
    ..have also analyzed the effect of two active-site mutations, D381A and Y440A upon the denaturation pathway of transketolase using intrinsic fluorescence measurements, and we compare the results from classical and microplate-based ..
  26. Langbein S, Frederiks W, zur Hausen A, Popa J, Lehmann J, Weiss C, et al. Metastasis is promoted by a bioenergetic switch: new targets for progressive renal cell cancer. Int J Cancer. 2008;122:2422-8 pubmed publisher
    ..branch by glucose-6-phosphate-dehydrogenase (G6PD) activity, and in the nonoxidative branch by the total transketolase activity and the specific expression of the transketolase-like-1 (TKTL1) protein...
  27. Mitschke L, Parthier C, Schröder Tittmann K, Coy J, Lüdtke S, Tittmann K. The crystal structure of human transketolase and new insights into its mode of action. J Biol Chem. 2010;285:31559-70 pubmed publisher
    The crystal structure of human transketolase (TKT), a thiamine diphosphate (ThDP) and Ca(2+)-dependent enzyme that catalyzes the interketol transfer between ketoses and aldoses as part of the pentose phosphate pathway, has been ..
  28. Liu H, Huang D, McArthur D, Boros L, Nissen N, Heaney A. Fructose induces transketolase flux to promote pancreatic cancer growth. Cancer Res. 2010;70:6368-76 pubmed publisher
    ..and glucose metabolism are quite different; in comparison with glucose, fructose induces thiamine-dependent transketolase flux and is preferentially metabolized via the nonoxidative pentose phosphate pathway to synthesize nucleic ..
  29. Díaz Moralli S, Tarrado Castellarnau M, Alenda C, Castells A, Cascante M. Transketolase-like 1 expression is modulated during colorectal cancer progression and metastasis formation. PLoS ONE. 2011;6:e25323 pubmed publisher
    b>Transketolase-like 1 (TKTL1) induces glucose degradation through anaerobic pathways, even in presence of oxygen, favoring the malignant aerobic glycolytic phenotype characteristic of tumor cells...
  30. Kamada N, Yasuhara A, Takano Y, Nakano T, Ikeda M. Effect of transketolase modifications on carbon flow to the purine-nucleotide pathway in Corynebacterium ammoniagenes. Appl Microbiol Biotechnol. 2001;56:710-7 pubmed
    b>Transketolase, one of the enzymes in the nonoxidative branch of the pentose phosphate pathway, operates to shuttle ribose 5-phosphate and glycolytic intermediates together with transaldolase, and might be involved in the availability of ..
  31. Iida A, Teshiba S, Mizobuchi K. Identification and characterization of the tktB gene encoding a second transketolase in Escherichia coli K-12. J Bacteriol. 1993;175:5375-83 pubmed
    ..Characterization of the mutant revealed that the level of the transketolase activity was reduced to one-third of that of the wild type. The mutation was mapped at 63.5 min on the E...
  32. Lovato L, Cianti R, Gini B, Marconi S, Bianchi L, Armini A, et al. Transketolase and 2',3'-cyclic-nucleotide 3'-phosphodiesterase type I isoforms are specifically recognized by IgG autoantibodies in multiple sclerosis patients. Mol Cell Proteomics. 2008;7:2337-49 pubmed publisher
    ..all MuS patients had cerebrospinal fluid IgG directed to isoforms of one of the oligodendroglial molecules, transketolase, 2',3'-cyclic-nucleotide 3'-phosphodiesterase type I, collapsin response mediator protein 2, and tubulin beta ..
  33. Thomas A, De Meese J, Le Huerou Y, Boyd S, Romoff T, Gonzales S, et al. Non-charged thiamine analogs as inhibitors of enzyme transketolase. Bioorg Med Chem Lett. 2008;18:509-12 pubmed publisher
    Inhibition of the thiamine-utilizing enzyme transketolase (TK) has been linked with diminished tumor cell proliferation...
  34. Boros L, Puigjaner J, Cascante M, Lee W, Brandes J, Bassilian S, et al. Oxythiamine and dehydroepiandrosterone inhibit the nonoxidative synthesis of ribose and tumor cell proliferation. Cancer Res. 1997;57:4242-8 pubmed
    ..investigates the significance of the glucose-6-phosphate dehydrogenase (G6PD) catalyzed oxidative and the transketolase (TK) catalyzed nonoxidative pentose cycle (PC) reactions in the tumor proliferation process by characterizing ..
  35. Comin Anduix B, Boren J, Martinez S, Moro C, Centelles J, Trebukhina R, et al. The effect of thiamine supplementation on tumour proliferation. A metabolic control analysis study. Eur J Biochem. 2001;268:4177-82 pubmed
    ..Thiamine (vitamin B1) is metabolized to thiamine pyrophosphate, the cofactor of transketolase, which is involved in ribose synthesis, necessary for cell replication...
  36. Salamon C, Chervenak M, Piatigorsky J, Sax C. The mouse transketolase (TKT) gene: cloning, characterization, and functional promoter analysis. Genomics. 1998;48:209-20 pubmed
    The transketolase (TKT) gene is expressed 30-50 times more highly in the mature mouse cornea than in other tissues. Here, we have cloned and characterized the 30- to 40-kb single-copy mouse TKT gene...
  37. Hammes H, Du X, Edelstein D, Taguchi T, Matsumura T, Ju Q, et al. Benfotiamine blocks three major pathways of hyperglycemic damage and prevents experimental diabetic retinopathy. Nat Med. 2003;9:294-9 pubmed
    ..as well as hyperglycemia-associated NF-kappaB activation, by activating the pentose phosphate pathway enzyme transketolase, which converts glyceraldehyde-3-phosphate and fructose-6-phosphate into pentose-5-phosphates and other sugars...
  38. Obrenovich M, Shamberger R, Lonsdale D. Altered heavy metals and transketolase found in autistic spectrum disorder. Biol Trace Elem Res. 2011;144:475-86 pubmed publisher
    ..Altered thiol metabolism from heavy metal toxicity may be responsible for the biochemical alterations in transketolase, and are mechanisms for oxidative stress production, dysautonomia, and abnormal thiamine homeostasis...
  39. Gerhardt S, Echt S, Busch M, Freigang J, Auerbach G, Bader G, et al. Structure and properties of an engineered transketolase from maize. Plant Physiol. 2003;132:1941-9 pubmed
    The gene specifying plastid transketolase (TK) of maize (Zea mays) was cloned from a cDNA library by southern blotting using a heterologous probe from sorghum (Sorghum bicolor)...
  40. Fiedler E, Thorell S, Sandalova T, Golbik R, König S, Schneider G. Snapshot of a key intermediate in enzymatic thiamin catalysis: crystal structure of the alpha-carbanion of (alpha,beta-dihydroxyethyl)-thiamin diphosphate in the active site of transketolase from Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2002;99:591-5 pubmed
    ..that addition of the donor substrate hydroxypyruvate to the thiamin diphosphate (ThDP)-dependent enzyme transketolase (TK) led to the accumulation of the alpha-carbanion/enamine of (alpha,beta-dihydroxyethyl) ThDP, the key ..
  41. Pácal L, Tomandl J, Svojanovsky J, Krusová D, Stepankova S, Rehorová J, et al. Role of thiamine status and genetic variability in transketolase and other pentose phosphate cycle enzymes in the progression of diabetic nephropathy. Nephrol Dial Transplant. 2011;26:1229-36 pubmed publisher
    ..and erythrocyte levels of thiamine and its esters) together with genetic variability in key PPP enzymes-transketolase (TKT), transaldolase and TKT-like-might contribute to the progression of diabetic nephropathy (DN) and ..
  42. Selivanov V, Kovina M, Kochevova N, Meshalkina L, Kochetov G. Kinetic study of the H103A mutant yeast transketolase. FEBS Lett. 2004;567:270-4 pubmed
    ..of holotransketolase (holoTK) does not come into contact with thiamin diphosphate (ThDP) but stabilizes the transketolase (TK) reaction intermediate, alpha,beta-dihydroxyethyl-thiamin diphosphate, by forming a hydrogen bond with the ..
  43. Boros L. Population thiamine status and varying cancer rates between western, Asian and African countries. Anticancer Res. 2000;20:2245-8 pubmed
    ..in nucleic acid ribose synthesis of tumor cells in its biologically activated form through the non-oxidative transketolase catalyzed pentose cycle reaction...
  44. Schmidt M, Voelker H, Kapp M, Krockenberger M, Dietl J, Kammerer U. Glycolytic phenotype in breast cancer: activation of Akt, up-regulation of GLUT1, TKTL1 and down-regulation of M2PK. J Cancer Res Clin Oncol. 2010;136:219-25 pubmed publisher
    ..Surprisingly, M2PK expression was highest in normal breast tissue. We found a glycolytic phenotype in a high percentage of breast cancer samples. Inhibition of glycolysis might evolve as a future option for breast cancer therapy. ..
  45. Wikner C, Nilsson U, Meshalkina L, Udekwu C, Lindqvist Y, Schneider G. Identification of catalytically important residues in yeast transketolase. Biochemistry. 1997;36:15643-9 pubmed
    The possible roles of four histidine residues in the active site of yeast transketolase were examined by site-directed mutagenesis. Replacement of the invariant His69 with alanine yielded a mutant enzyme with 1...
  46. Asztalos P, Parthier C, Golbik R, Kleinschmidt M, Hübner G, Weiss M, et al. Strain and near attack conformers in enzymic thiamin catalysis: X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor. Biochemistry. 2007;46:12037-52 pubmed
    b>Transketolase is a prominent thiamin diphosphate-dependent enzyme in sugar metabolism that catalyzes the reversible transfer of a 2-carbon dihydroxyethyl fragment between a donor ketose and an acceptor aldose...
  47. Harinarayanan R, Murphy H, Cashel M. Synthetic growth phenotypes of Escherichia coli lacking ppGpp and transketolase A (tktA) are due to ppGpp-mediated transcriptional regulation of tktB. Mol Microbiol. 2008;69:882-94 pubmed publisher
    ..a random screen for synthetic lethals we found a ppGpp-dependent functional pathway that operates through transketolase B (TktB), and which is 'buffered' in wildtype strain by the presence of an isozyme, transketolase A (TktA)...
  48. Feyen O, Coy J, Prasad V, Schierl R, Saenger J, Baum R. EDIM-TKTL1 blood test: a noninvasive method to detect upregulated glucose metabolism in patients with malignancies. Future Oncol. 2012;8:1349-59 pubmed publisher
    ..EDIM-TKTL1 blood test revealed good concordance with FDG-PET/CT results in patients with malignancies demonstrating its efficacy to detect upregulation of glucose metabolism in primary tumors or metastases. ..
  49. Frederiks W, Vizan P, Bosch K, Vreeling Sindelárová H, Boren J, Cascante M. Elevated activity of the oxidative and non-oxidative pentose phosphate pathway in (pre)neoplastic lesions in rat liver. Int J Exp Pathol. 2008;89:232-40 pubmed publisher
    ..b>Transketolase (TKT), the reversible rate-limiting enzyme of the non-oxidative branch of the PPP, mainly responsible for ..
  50. Soukaloun D, Lee S, Chamberlain K, Taylor A, Mayxay M, Sisouk K, et al. Erythrocyte transketolase activity, markers of cardiac dysfunction and the diagnosis of infantile beriberi. PLoS Negl Trop Dis. 2011;5:e971 pubmed publisher
    ..Basal and activated erythrocyte transketolase activities (ETK) and activation (?) coefficients were assayed along with plasma brain natriuretic peptide, N-..
  51. Du M, Sim J, Fang L, Yin Z, Koh S, Stratton J, et al. Identification of novel small-molecule inhibitors for human transketolase by high-throughput screening with fluorescent intensity (FLINT) assay. J Biomol Screen. 2004;9:427-33 pubmed
    The metabolic enzyme transketolase (TK) plays a crucial role in tumor cell nucleic acid synthesis, using glucose through the elevated nonoxidative pentose phosphate pathway (PPP)...
  52. Obiol Pardo C, Rubio Martinez J. Homology modeling of human transketolase: description of critical sites useful for drug design and study of the cofactor binding mode. J Mol Graph Model. 2009;27:723-34 pubmed publisher
    b>Transketolase, the most critical enzyme of the non-oxidative branch of the pentose phosphate pathway, has been reported as a new target protein for cancer research...