Summary: Molecules that contain multiple active sites which are used to catalyze more than one enzymatic reaction. Proteins in this class generally contain multiple active sites within a single peptide chain and may also contain more than one enzymatically active subunit. They are distinguished from MULTIENZYME COMPLEXES in that their subunits are not found as distinct enzymes.
Keen B, Bailey L, Jozwiakowski S, Doherty A. Author response: PRIMPOL mutation: functional study does not always reveal the truth. Invest Ophthalmol Vis Sci. 2015;56:1183 pubmed publisher
Bailey L, Doherty A. Mitochondrial DNA replication: a PrimPol perspective. Biochem Soc Trans. 2017;45:513-529 pubmed publisher
..Here, we discuss what is currently known about the mechanisms of DNA replication in the mitochondrion, the proteins that carry out these processes and how PrimPol is likely to be involved in assisting this vital cellular process. ..
Moxley M, Zhang L, Christgen S, Tanner J, Becker D. Identification of a Conserved Histidine As Being Critical for the Catalytic Mechanism and Functional Switching of the Multifunctional Proline Utilization A Protein. Biochemistry. 2017;56:3078-3088 pubmed publisher
..These results indicate that His487 is critically important for the proline/P5C chemical step, conformational change kinetics, and functional switching in EcPutA. ..
Peralta Castro A, Baruch Torres N, Brieba L. Plant organellar DNA primase-helicase synthesizes RNA primers for organellar DNA polymerases using a unique recognition sequence. Nucleic Acids Res. 2017;45:10764-10774 pubmed publisher
..This mechanism contrasts to lagging-strand DNA replication in metazoan mitochondria, in which transcripts synthesized by mitochondrial RNA polymerase prime mitochondrial DNA polymerase ?. ..
Li J, Zhang Q. PRIMPOL mutation: functional study does not always reveal the truth. Invest Ophthalmol Vis Sci. 2015;56:1181-2 pubmed publisher
Geng X, Li W, Chen Z, Gao S, Hong W, Ge X, et al
. The Bifunctional Enzyme SpoT Is Involved in the Clarithromycin Tolerance of Helicobacter pylori by Upregulating the Transporters HP0939, HP1017, HP0497, and HP0471. Antimicrob Agents Chemother. 2017;61: pubmed publisher
..Taken together, our results revealed a novel molecular mechanism of H. pylori adaption to CLA stress. ..
Deshpande A, Pochapsky T, Ringe D. The Metal Drives the Chemistry: Dual Functions of Acireductone Dioxygenase. Chem Rev. 2017;117:10474-10501 pubmed publisher
Boldinova E, Stojkovič G, Khairullin R, Wanrooij S, Makarova A. Optimization of the expression, purification and polymerase activity reaction conditions of recombinant human PrimPol. PLoS ONE. 2017;12:e0184489 pubmed publisher
..Conversely, the yield of full-length protein expressed in S. cerevisiae was considerably lower and this system is therefore not recommended for expression of full-length recombinant human PrimPol. ..
Agudo R, Calvo P, Martinez Jimenez M, Blanco L. Engineering human PrimPol into an efficient RNA-dependent-DNA primase/polymerase. Nucleic Acids Res. 2017;45:9046-9058 pubmed publisher
..Finally, in support of the biotechnological potential of PrimPol as a DNA primer maker during reverse transcription, mutant Y89R HsPrimPol rendered up to 17-fold more DNA than with random hexamer primers. ..