Genomes and Genes
light harvesting protein complexes
Summary: Complexes containing CHLOROPHYLL and other photosensitive molecules. They serve to capture energy in the form of PHOTONS and are generally found as components of the PHOTOSYSTEM I PROTEIN COMPLEX or the PHOTOSYSTEM II PROTEIN COMPLEX.
- Slavov C, Ballottari M, Morosinotto T, Bassi R, Holzwarth A. Trap-limited charge separation kinetics in higher plant photosystem I complexes. Biophys J. 2008;94:3601-12 pubmed publisher..The influence of the red Chls on the slowing of the overall trapping kinetics in the intact PS I complex is estimated to be approximately four times larger than the effect of the bulk antenna enlargement. ..
- Ruban A, Johnson M. Dynamics of higher plant photosystem cross-section associated with state transitions. Photosynth Res. 2009;99:173-83 pubmed publisher..These alterations reveal remarkable plasticity of the higher plant photosynthetic antenna design providing the basis for a flexible adaptation to the light environment. ..
- Kirchhoff H, Haase W, Wegner S, Danielsson R, Ackermann R, Albertsson P. Low-light-induced formation of semicrystalline photosystem II arrays in higher plant chloroplasts. Biochemistry. 2007;46:11169-76 pubmed..Furthermore, the occurrence of a hexagonal phase of the lipid monogalactosyldiacylglycerol in grana membranes of low-light-adapted plants could trigger the rearrangement by changing the lateral membrane pressure...
- Six C, Thomas J, Garczarek L, Ostrowski M, Dufresne A, Blot N, et al. Diversity and evolution of phycobilisomes in marine Synechococcus spp.: a comparative genomics study. Genome Biol. 2007;8:R259 pubmed..Genomes of eleven marine Synechococcus strains recently became available with one to four strains per pigment type or subtype, allowing an unprecedented comparative genomics study of genes involved in phycobilisome metabolism...
- Koziol A, Borza T, Ishida K, Keeling P, Lee R, Durnford D. Tracing the evolution of the light-harvesting antennae in chlorophyll a/b-containing organisms. Plant Physiol. 2007;143:1802-16 pubmed..This analysis provides a snapshot of the antenna systems in diverse green algae, and allows us to infer the changing complexity of the antenna system during green algal evolution. ..
- Matsubara S, Morosinotto T, Osmond C, Bassi R. Short- and long-term operation of the lutein-epoxide cycle in light-harvesting antenna complexes. Plant Physiol. 2007;144:926-41 pubmed..These results are discussed in the context of photoacclimation and shade adaptation. ..
- Lepetit B, Volke D, Szabó M, Hoffmann R, Garab G, Wilhelm C, et al. Spectroscopic and molecular characterization of the oligomeric antenna of the diatom Phaeodactylum tricornutum. Biochemistry. 2007;46:9813-22 pubmed..The presence of an oligomeric antenna in diatoms is in line with the oligomeric organization of antenna complexes in different photoautotrophic groups. ..
- Horn R, Grundmann G, Paulsen H. Consecutive binding of chlorophylls a and b during the assembly in vitro of light-harvesting chlorophyll-a/b protein (LHCIIb). J Mol Biol. 2007;366:1045-54 pubmed
- Betterle N, Ballottari M, Zorzan S, de Bianchi S, Cazzaniga S, Dall Osto L, et al. Light-induced dissociation of an antenna hetero-oligomer is needed for non-photochemical quenching induction. J Biol Chem. 2009;284:15255-66 pubmed publisher..These changes are reversible and do not require protein synthesis/degradation, thus allowing for changes in PSII antenna size and adaptation to rapidly changing environmental conditions. ..
- Ballottari M, Dall Osto L, Morosinotto T, Bassi R. Contrasting behavior of higher plant photosystem I and II antenna systems during acclimation. J Biol Chem. 2007;282:8947-58 pubmed
- Ruban A, Berera R, Ilioaia C, van Stokkum I, Kennis J, Pascal A, et al. Identification of a mechanism of photoprotective energy dissipation in higher plants. Nature. 2007;450:575-8 pubmed..We suggest that this is the principal mechanism of photoprotection...
- Voronine D, Abramavicius D, Mukamel S. Chirality-based signatures of local protein environments in two-dimensional optical spectroscopy of two species photosynthetic complexes of green sulfur bacteria: simulation study. Biophys J. 2008;95:4896-907 pubmed publisher..Pulse polarization configurations are designed that can separate the coherent and incoherent exciton dynamics contributions to the two-dimensional spectra. ..
- Croce R, Mozzo M, Morosinotto T, Romeo A, Hienerwadel R, Bassi R. Singlet and triplet state transitions of carotenoids in the antenna complexes of higher-plant photosystem I. Biochemistry. 2007;46:3846-55 pubmed..This suggests that in the Lhca2-Lhca3 heterodimeric complex energy equilibration is not complete at least on a fast time scale. ..
- Kavalenka A, Spruijt R, Wolfs C, Strancar J, Croce R, Hemminga M, et al. Site-directed spin-labeling study of the light-harvesting complex CP29. Biophys J. 2009;96:3620-8 pubmed publisher..On the other hand, position 15 is located in a flexible region, relatively far away from the transmembrane domain. ..
- Dall Osto L, Fiore A, Cazzaniga S, Giuliano G, Bassi R. Different roles of alpha- and beta-branch xanthophylls in photosystem assembly and photoprotection. J Biol Chem. 2007;282:35056-68 pubmed
- Caffarri S, Passarini F, Bassi R, Croce R. A specific binding site for neoxanthin in the monomeric antenna proteins CP26 and CP29 of Photosystem II. FEBS Lett. 2007;581:4704-10 pubmed..In contrast to previous proposals, it is thus concluded that also in these minor antenna complexes neoxanthin is accommodated in the N1 site. The characteristics of this binding site in the different antenna complexes are discussed. ..
- Wen J, Zhang H, Gross M, Blankenship R. Membrane orientation of the FMO antenna protein from Chlorobaculum tepidum as determined by mass spectrometry-based footprinting. Proc Natl Acad Sci U S A. 2009;106:6134-9 pubmed publisher..tepidum) and give information on the packing of the FMO protein in its native environment. ..
- Schmid V. Light-harvesting complexes of vascular plants. Cell Mol Life Sci. 2008;65:3619-39 pubmed publisher..Therefore, topics for the next decade will be the elucidation of the location(s) and the operating mode of steps in the assembly and degradation process. ..
- Ihnatowicz A, Pesaresi P, Lohrig K, Wolters D, Müller B, Leister D. Impaired photosystem I oxidation induces STN7-dependent phosphorylation of the light-harvesting complex I protein Lhca4 in Arabidopsis thaliana. Planta. 2008;227:717-22 pubmed..Thus, under extreme redox conditions, hyperactivation of thylakoid protein kinases and/or reorganization of thylakoid protein complex distribution increase the susceptibility of PSI to phosphorylation. ..
- Alboresi A, Caffarri S, Nogue F, Bassi R, Morosinotto T. In silico and biochemical analysis of Physcomitrella patens photosynthetic antenna: identification of subunits which evolved upon land adaptation. PLoS ONE. 2008;3:e2033 pubmed publisher..The moss Physcomitrella patens is a member of a lineage that diverged from seed plants early after land colonization and therefore by studying this organism, we may gain insight into adaptations to the aerial environment...
- Schluchter W, Shen G, Alvey R, Biswas A, Saunee N, Williams S, et al. Phycobiliprotein biosynthesis in cyanobacteria: structure and function of enzymes involved in post-translational modification. Adv Exp Med Biol. 2010;675:211-28 pubmed publisher..This structure shows that the bilin lyases are most similar to the lipocalin protein structural family, which also includes the bilin-binding protein found in some butterflies. ..
- Garczarek L, Dufresne A, Rousvoal S, West N, Mazard S, Marie D, et al. High vertical and low horizontal diversity of Prochlorococcus ecotypes in the Mediterranean Sea in summer. FEMS Microbiol Ecol. 2007;60:189-206 pubmed..Nevertheless, environmental pcb gene sequences retrieved from different depths at two stations proved all different at the nucleotide level, suggesting a large genetic microdiversity within those ecotypes. ..
- Wormit M, Dreuw A. Quantum chemical insights in energy dissipation and carotenoid radical cation formation in light harvesting complexes. Phys Chem Chem Phys. 2007;9:2917-31 pubmed..By comparison of theoretical findings with recent experimental data, a general mechanism for carotenoid radical cation formation is suggested. ..
- Horigome D, Satoh H, Itoh N, Mitsunaga K, Oonishi I, Nakagawa A, et al. Structural mechanism and photoprotective function of water-soluble chlorophyll-binding protein. J Biol Chem. 2007;282:6525-31 pubmed..With reference to the novel Chl-binding mode, we propose that the photoprotection mechanism may be based on the inhibition of physical contact between the Chl molecules and molecular oxygen. ..
- Singh A, Sherman L. Reflections on the function of IsiA, a cyanobacterial stress-inducible, Chl-binding protein. Photosynth Res. 2007;93:17-25 pubmed..The de facto transcriptional control of isiA expression has expanded to include regulation at both the transcriptional and post-transcriptional levels. ..
- Wang Q, Jantaro S, Lu B, Majeed W, Bailey M, He Q. The high light-inducible polypeptides stabilize trimeric photosystem I complex under high light conditions in Synechocystis PCC 6803. Plant Physiol. 2008;147:1239-50 pubmed publisher..These results suggest that the HLIPs stabilize PSI trimers, interact with Slr1128, and protect cells under HL conditions. ..
- Cheng Y, Ahn T, Avenson T, Zigmantas D, Niyogi K, Ballottari M, et al. Kinetic modeling of charge-transfer quenching in the CP29 minor complex. J Phys Chem B. 2008;112:13418-23 pubmed publisher..Finally, we compare simulations of CT quenching in thylakoids with those of the individual CP29 complexes and propose that CP29 rather than LHCII is a site of CT quenching. ..
- Read E, Schlau Cohen G, Engel G, Wen J, Blankenship R, Fleming G. Visualization of excitonic structure in the Fenna-Matthews-Olson photosynthetic complex by polarization-dependent two-dimensional electronic spectroscopy. Biophys J. 2008;95:847-56 pubmed publisher..The results suggest the possibility of designing experiments using combinations of tailored polarization sequences to separate and monitor individual relaxation pathways. ..
- Frigerio S, Campoli C, Zorzan S, Fantoni L, Crosatti C, Drepper F, et al. Photosynthetic antenna size in higher plants is controlled by the plastoquinone redox state at the post-transcriptional rather than transcriptional level. J Biol Chem. 2007;282:29457-69 pubmed..We conclude that the plastoquinone redox state plays an important role in the long term regulation of chloroplast protein expression. However, its modulation is active at the post-transcriptional rather than transcriptional level. ..
- Psencik J, Collins A, Liljeroos L, Torkkeli M, Laurinmäki P, Ansink H, et al. Structure of chlorosomes from the green filamentous bacterium Chloroflexus aurantiacus. J Bacteriol. 2009;191:6701-8 pubmed publisher..The wider lamellae allow accommodation of the additional carotenoids and lead to increased disorder within the lamellae...
- Tikkanen M, Nurmi M, Suorsa M, Danielsson R, Mamedov F, Styring S, et al. Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants. Biochim Biophys Acta. 2008;1777:425-32 pubmed publisher..The grana margins probably give a flexibility for regulation of linear and cyclic electron flow in plant chloroplasts. ..
- Tzvetkova Chevolleau T, Hutin C, Noël L, Goforth R, Carde J, Caffarri S, et al. Canonical signal recognition particle components can be bypassed for posttranslational protein targeting in chloroplasts. Plant Cell. 2007;19:1635-48 pubmed
- Kim H, Li H, Maresca J, Bryant D, Savikhin S. Triplet exciton formation as a novel photoprotection mechanism in chlorosomes of Chlorobium tepidum. Biophys J. 2007;93:192-201 pubmed..Thus, the formation of triplet excitons in chlorosomes serves as an alternative photoprotection mechanism...
- Kirchhoff H, Lenhert S, Büchel C, Chi L, Nield J. Probing the organization of photosystem II in photosynthetic membranes by atomic force microscopy. Biochemistry. 2008;47:431-40 pubmed..The functional consequences for lateral migration processes are discussed...
- Kim E, Li X, Razeghifard R, Anderson J, Niyogi K, Pogson B, et al. The multiple roles of light-harvesting chlorophyll a/b-protein complexes define structure and optimize function of Arabidopsis chloroplasts: a study using two chlorophyll b-less mutants. Biochim Biophys Acta. 2009;1787:973-84 pubmed publisher..The evolution of chlorophyll b-containing chloroplasts seems to fine-tune oxygenic photosynthesis. ..
- Barros T, Kuhlbrandt W. Crystallisation, structure and function of plant light-harvesting Complex II. Biochim Biophys Acta. 2009;1787:753-72 pubmed publisher..Finally, some recently proposed mechanisms of energy-dependent non-photochemical quenching (NPQ) are examined from a structural perspective. ..
- Zhao K, Su P, Tu J, Wang X, Liu H, Plöscher M, et al. Phycobilin:cystein-84 biliprotein lyase, a near-universal lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins. Proc Natl Acad Sci U S A. 2007;104:14300-5 pubmed
- Kovacs L, Damkjaer J, Kereïche S, Ilioaia C, Ruban A, Boekema E, et al. Lack of the light-harvesting complex CP24 affects the structure and function of the grana membranes of higher plant chloroplasts. Plant Cell. 2006;18:3106-20 pubmed
- Rochaix J. Role of thylakoid protein kinases in photosynthetic acclimation. FEBS Lett. 2007;581:2768-75 pubmed..These kinases appear to be involved both in short and long term adaptations to changes in the light environment. ..
- Chen M, Zhang Y, Blankenship R. Nomenclature for membrane-bound light-harvesting complexes of cyanobacteria. Photosynth Res. 2008;95:147-54 pubmed..The CBP complexes are a member of a larger family that includes the chlorophyll a-binding proteins CP43 and CP47 that function as core antennas of photosystem II. ..
- Tronrud D, Wen J, Gay L, Blankenship R. The structural basis for the difference in absorbance spectra for the FMO antenna protein from various green sulfur bacteria. Photosynth Res. 2009;100:79-87 pubmed publisher..This difference in binding is shown to be predictive of the spectral type of the FMO...
- Dammeyer T, Frankenberg Dinkel N. Insights into phycoerythrobilin biosynthesis point toward metabolic channeling. J Biol Chem. 2006;281:27081-9 pubmed..bilin complexes. A combination of substrate/product binding analyses and gel permeation chromatography revealed evidence for metabolic channeling. ..
- Standfuss J, Kuhlbrandt W. The three isoforms of the light-harvesting complex II: spectroscopic features, trimer formation, and functional roles. J Biol Chem. 2004;279:36884-91 pubmed..The most likely role of Lhcb3 is as an intermediary in light energy transfer from the main Lhcb1/Lhcb2 antenna to the photosystem II core. ..
- Morosinotto T, Ballottari M, Klimmek F, Jansson S, Bassi R. The association of the antenna system to photosystem I in higher plants. Cooperative interactions stabilize the supramolecular complex and enhance red-shifted spectral forms. J Biol Chem. 2005;280:31050-8 pubmed..When compared with Photosystem II, the association of peripheral antenna complexes in PSI appears to be more stable, but far less flexible and functional implications are discussed. ..
- Kouril R, Arteni A, Lax J, Yeremenko N, D Haene S, Rögner M, et al. Structure and functional role of supercomplexes of IsiA and Photosystem I in cyanobacterial photosynthesis. FEBS Lett. 2005;579:3253-7 pubmed..Work on mutants indicates that the PsaF/J and PsaL subunits facilitate the formation of closed rings around Photosystem I monomers but are not obligatory components in the formation of Photosystem I-IsiA supercomplexes. ..
- Bahatyrova S, Frese R, Siebert C, Olsen J, Van Der Werf K, van Grondelle R, et al. The native architecture of a photosynthetic membrane. Nature. 2004;430:1058-62 pubmed
- Ihalainen J, Croce R, Morosinotto T, van Stokkum I, Bassi R, Dekker J, et al. Excitation decay pathways of Lhca proteins: a time-resolved fluorescence study. J Phys Chem B. 2005;109:21150-8 pubmed
- Rutkauskas D, Olsen J, Gall A, Cogdell R, Hunter C, van Grondelle R. Comparative study of spectral flexibilities of bacterial light-harvesting complexes: structural implications. Biophys J. 2006;90:2463-74 pubmed..The differences in spectral diffusion are associated with subtle differences of the binding pocket of B850 pigments and the structural flexibility of the different types of complexes...
- Pascal A, Liu Z, Broess K, van Oort B, van Amerongen H, Wang C, et al. Molecular basis of photoprotection and control of photosynthetic light-harvesting. Nature. 2005;436:134-7 pubmed..This provides a molecular basis for understanding the control of photosynthetic light-harvesting. ..
- Standfuss J, Terwisscha van Scheltinga A, Lamborghini M, Kuhlbrandt W. Mechanisms of photoprotection and nonphotochemical quenching in pea light-harvesting complex at 2.5 A resolution. EMBO J. 2005;24:919-28 pubmed..Our structure shows the complex in a quenched state, which may be relevant for the rapid, pH-induced component of nonphotochemical quenching. ..