glycine decarboxylase complex


Summary: A enzyme complex that catalyzes the oxidative DECARBOXYLATION and DEAMINATION of GLYCINE into CARBON DIOXIDE; AMMONIA; NADH; and N5N10-methylenetetrahydrofolate. It is composed of four different component protein components referred to as H, P, L, and T.

Top Publications

  1. Mukherjee M, Brown M, McArthur A, Johnson P. Proteins of the glycine decarboxylase complex in the hydrogenosome of Trichomonas vaginalis. Eukaryot Cell. 2006;5:2062-71 pubmed
    ..of two glycine cleavage H proteins and a dihydrolipoamide dehydrogenase (L protein) of the glycine decarboxylase complex in T. vaginalis with predicted N-terminal hydrogenosomal presequences...
  2. Rajinikanth M, Harding S, Tsai C. The glycine decarboxylase complex multienzyme family in Populus. J Exp Bot. 2007;58:1761-70 pubmed
    In plants, the glycine decarboxylase complex (GDC) cooperates with serine hydroxymethyltransferase (SHMT) to mediate photorespiratory glycine-serine interconversion...
  3. Hagemann M, Vinnemeier J, Oberpichler I, Boldt R, Bauwe H. The glycine decarboxylase complex is not essential for the cyanobacterium Synechocystis sp. strain PCC 6803. Plant Biol (Stuttg). 2005;7:15-22 pubmed
    ..It appears that, in contrast to heterotrophic bacteria and plants, GDC is dispensable for Synechocystis and possibly other cyanobacteria. ..
  4. Gelling C, Piper M, Hong S, Kornfeld G, Dawes I. Identification of a novel one-carbon metabolism regulon in Saccharomyces cerevisiae. J Biol Chem. 2004;279:7072-81 pubmed
    Glycine specifically induces genes encoding subunits of the glycine decarboxylase complex (GCV1, GCV2, and GCV3), and this is mediated by a fall in cytoplasmic levels of 5,10-methylenetetrahydrofolate caused by inhibition of cytoplasmic ..
  5. Zhou Q, Yu Q, Wang Z, Pan Y, Lv W, Zhu L, et al. Knockdown of GDCH gene reveals reactive oxygen species-induced leaf senescence in rice. Plant Cell Environ. 2013;36:1476-89 pubmed publisher
    b>Glycine decarboxylase complex (GDC) is a multi-protein complex, comprising P-, H-, T- and L-protein subunits, which plays a major role in photorespiration in plants...
  6. Spalding M, Allary M, Gallagher J, Prigge S. Validation of a modified method for Bxb1 mycobacteriophage integrase-mediated recombination in Plasmodium falciparum by localization of the H-protein of the glycine cleavage complex to the mitochondrion. Mol Biochem Parasitol. 2010;172:156-60 pubmed publisher
    ..Here, we demonstrate that this modification results in rapid generation of chromosomally integrated transgenic parasites, and we show that the H-protein localizes to the mitochondrion. ..
  7. Muller M, Papadopoulou B. Stage-specific expression of the glycine cleavage complex subunits in Leishmania infantum. Mol Biochem Parasitol. 2010;170:17-27 pubmed publisher
    ..Altogether, these data suggest a complex and multilayered regulation of the GCC both at the mRNA and protein levels throughout the L. infantum life cycle. ..
  8. Bagard M, Le Thiec D, Delacote E, Hasenfratz Sauder M, Banvoy J, GĂ©rard J, et al. Ozone-induced changes in photosynthesis and photorespiration of hybrid poplar in relation to the developmental stage of the leaves. Physiol Plant. 2008;134:559-74 pubmed publisher
    ..of serine hydroxymethyltransferase in treated mature leaves, while the content of the H subunit of the glycine decarboxylase complex was not modified...
  9. Wang Y, Harding S, Tsai C. Expression of a glycine decarboxylase complex H-protein in non-photosynthetic tissues of Populus tremuloides. Biochim Biophys Acta. 2004;1676:266-72 pubmed
    ..Strong expression of PtgdcH1 in root tips and developing xylem suggests that GDC supports a very active C1 metabolism in non-photosynthetic tissues of aspen. ..

More Information


  1. Macherel D, Bourguignon J, Forest E, Faure M, Cohen Addad C, Douce R. Expression, lipoylation and structure determination of recombinant pea H-protein in Escherichia coli. Eur J Biochem. 1996;236:27-33 pubmed
    A synthetic gene encoding the entire mature H protein of the glycine decarboxylase complex from pea (Pisum sativum L.) was constructed and expressed in Escherichia coli...
  2. Taylor N, Day D, Millar A. Environmental stress causes oxidative damage to plant mitochondria leading to inhibition of glycine decarboxylase. J Biol Chem. 2002;277:42663-8 pubmed
    ..The most dramatic loss of antigenicity was seen with the 17-kDa glycine decarboxylase complex (GDC) H-protein, which was correlated with the loss of glycine-dependent O2 consumption...
  3. Piper M, Hong S, Ball G, Dawes I. Regulation of the balance of one-carbon metabolism in Saccharomyces cerevisiae. J Biol Chem. 2000;275:30987-95 pubmed
    ..control of the expression of key genes, particularly those encoding the unique components of the glycine decarboxylase complex (GCV1, GCV2, and GCV3)...
  4. Igamberdiev A, Bykova N, Gardestrom P. Involvement of cyanide-resistant and rotenone-insensitive pathways of mitochondrial electron transport during oxidation of glycine in higher plants. FEBS Lett. 1997;412:265-9 pubmed
    ..The involvement of different paths of electron transport in mitochondria during operation of glycine decarboxylase complex (GDC) was tested in different conditions, using aminoacetonitrile (AAN), the inhibitor of glycine ..
  5. Ferris P, Armbrust E, Goodenough U. Genetic structure of the mating-type locus of Chlamydomonas reinhardtii. Genetics. 2002;160:181-200 pubmed
    ..identified housekeeping genes include Pdk, encoding pyruvate dehydrogenase kinase, and GdcH, encoding glycine decarboxylase complex subunit H...
  6. Eisenhut M, Kahlon S, Hasse D, Ewald R, Lieman Hurwitz J, Ogawa T, et al. The plant-like C2 glycolate cycle and the bacterial-like glycerate pathway cooperate in phosphoglycolate metabolism in cyanobacteria. Plant Physiol. 2006;142:333-42 pubmed
    ..This finding and the growth data imply a dominant, although not exclusive, role of the C2 route in cyanobacterial phosphoglycolate metabolism. ..
  7. Faure M, Bourguignon J, Neuburger M, Macherel D, Sieker L, Ober R, et al. Interaction between the lipoamide-containing H-protein and the lipoamide dehydrogenase (L-protein) of the glycine decarboxylase multienzyme system 2. Crystal structures of H- and L-proteins. Eur J Biochem. 2000;267:2890-8 pubmed
    The glycine decarboxylase complex consists of four different component enzymes (P-, H-, T- and L-proteins)...
  8. Li J, Tong S, Wands J. Identification and expression of glycine decarboxylase (p120) as a duck hepatitis B virus pre-S envelope-binding protein. J Biol Chem. 1999;274:27658-65 pubmed
    ..amino acid sequences of tryptic peptides from purified p120 were found to be the duck p protein of the glycine decarboxylase complex (DGD)...
  9. Cohen Addad C, Faure M, Neuburger M, Ober R, Sieker L, Bourguignon J, et al. Structural studies of the glycine decarboxylase complex from pea leaf mitochondria. Biochimie. 1997;79:637-43 pubmed
    The glycine decarboxylase complex consists of four different component enzymes (P-, H-, T- and L-proteins)...
  10. Sinclair D, Hong S, Dawes I. Specific induction by glycine of the gene for the P-subunit of glycine decarboxylase from Saccharomyces cerevisiae. Mol Microbiol. 1996;19:611-23 pubmed
    The glycine decarboxylase complex (GDC) is composed of four subunits referred to as H-, L-, P-, and T-proteins...
  11. Narisawa A, Komatsuzaki S, Kikuchi A, Niihori T, Aoki Y, Fujiwara K, et al. Mutations in genes encoding the glycine cleavage system predispose to neural tube defects in mice and humans. Hum Mol Genet. 2012;21:1496-503 pubmed publisher
    ..Overall, our findings suggest that loss-of-function mutations in GCS genes predispose to NTDs in mice and humans. These data highlight the importance of adequate function of mitochondrial folate metabolism in neural tube closure...
  12. Prabhu V, Chatson K, Abrams G, King J. 13C nuclear magnetic resonance detection of interactions of serine hydroxymethyltransferase with C1-tetrahydrofolate synthase and glycine decarboxylase complex activities in Arabidopsis. Plant Physiol. 1996;112:207-16 pubmed
    ..photorespiratory glycine metabolism involving the tetrahydrofolate (THF)-dependent activities of the glycine decarboxylase complex (GDC) and serine hydroxymethyl transferase (SHMT)...