rab2 gtp binding protein


Summary: A protein involved in transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS. This enzyme was formerly listed as EC

Top Publications

  1. Tisdale E, Balch W. Rab2 is essential for the maturation of pre-Golgi intermediates. J Biol Chem. 1996;271:29372-9 pubmed
    ..The combined results suggest that the NH2 terminus of Rab2 is required for its function and for direct interaction with components of the transport machinery involved in the maturation of pre-Golgi intermediates. ..
  2. Tisdale E, Artalejo C. A GAPDH mutant defective in Src-dependent tyrosine phosphorylation impedes Rab2-mediated events. Traffic. 2007;8:733-41 pubmed
    ..Our results suggest that Src plays a pivotal role in regulating the interaction of Rab2 effectors on the VTC. ..
  3. Tisdale E. Rab2 interacts directly with atypical protein kinase C (aPKC) iota/lambda and inhibits aPKCiota/lambda-dependent glyceraldehyde-3-phosphate dehydrogenase phosphorylation. J Biol Chem. 2003;278:52524-30 pubmed
    ..These results suggest that a downstream effector recruited to the VTC stimulates PKCiota/lambda-mediated GAPDH phosphorylation by alleviating the inhibition imposed by Rab2-PKCiota/lambda interaction. ..
  4. Tisdale E. Rab2 purification and interaction with protein kinase C iota/lambda and glyceraldehyde-3-phosphate dehydrogenase. Methods Enzymol. 2005;403:381-91 pubmed
    ..Additionally, in vivo and in vitro assays are outlined that are employed to demonstrate Rab2 interaction with the downstream effectors aPKCiota/lambda and GAPDH. ..
  5. Buffa L, Fuchs E, Pietropaolo M, Barr F, Solimena M. ICA69 is a novel Rab2 effector regulating ER-Golgi trafficking in insulinoma cells. Eur J Cell Biol. 2008;87:197-209 pubmed publisher
    ..Taken together, these data identify ICA69 as a novel Rab2 effector and point to its role in regulating the early transport of insulin secretory granule proteins. ..
  6. Short B, Preisinger C, Korner R, Kopajtich R, Byron O, Barr F. A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic. J Cell Biol. 2001;155:877-83 pubmed
    ..These results demonstrate that GRASP55 and golgin-45 form a rab2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure. ..
  7. Tisdale E. A Rab2 mutant with impaired GTPase activity stimulates vesicle formation from pre-Golgi intermediates. Mol Biol Cell. 1999;10:1837-49 pubmed
    ..These data suggest that the Rab2 protein plays a role in the low-temperature-sensitive step that regulates membrane flow from VTCs to the Golgi complex and back to the ER. ..
  8. Tisdale E. Glyceraldehyde-3-phosphate dehydrogenase is phosphorylated by protein kinase Ciota /lambda and plays a role in microtubule dynamics in the early secretory pathway. J Biol Chem. 2002;277:3334-41 pubmed
    ..These combined results suggest that Rab2 stimulated PKCiota/lambda and GAPDH recruitment to VTCs, and the subsequent PKCiota/lambda phosphorylation of GAPDH ultimately influences MT dynamics in the early secretory pathway. ..
  9. Tisdale E. Glyceraldehyde-3-phosphate dehydrogenase is required for vesicular transport in the early secretory pathway. J Biol Chem. 2001;276:2480-6 pubmed
    ..These data suggest that GAPDH is required for ER to Golgi transport. We propose that membranes incubated with anti-GAPDH and Rab2 form "dead end" vesicles that are unable to transport and fuse with the acceptor compartment. ..

More Information


  1. Machner M, Isberg R. Targeting of host Rab GTPase function by the intravacuolar pathogen Legionella pneumophila. Dev Cell. 2006;11:47-56 pubmed
    ..These results indicate that proteins translocated into the host cell by the intravacuolar pathogen L. pneumophila are able to recapitulate events involved in host secretory trafficking. ..
  2. Mangahas P, Yu X, Miller K, Zhou Z. The small GTPase Rab2 functions in the removal of apoptotic cells in Caenorhabditis elegans. J Cell Biol. 2008;180:357-73 pubmed publisher
    ..We propose that UNC-108 acts on phagosomal surfaces to promote phagosome maturation and suggest that mammalian Rab2 may have a similar function in the degradation of apoptotic cells. ..
  3. Dong C, Wu G. Regulation of anterograde transport of adrenergic and angiotensin II receptors by Rab2 and Rab6 GTPases. Cell Signal. 2007;19:2388-99 pubmed
    ..These data also provide the first evidence indicating that Rab6-coordinated retrograde transport selectively modulates intracellular trafficking and signaling of GPCRs. ..
  4. Chun D, McEwen J, Burbea M, Kaplan J. UNC-108/Rab2 regulates postendocytic trafficking in Caenorhabditis elegans. Mol Biol Cell. 2008;19:2682-95 pubmed publisher
    ..These results define a new function for Rab2 in protein trafficking. ..
  5. Tisdale E, Jackson M. Rab2 protein enhances coatomer recruitment to pre-Golgi intermediates. J Biol Chem. 1998;273:17269-77 pubmed
    ..Our results suggest that the Rab2 peptide causes the persistent recruitment of COPI to pre-Golgi intermediates which ultimately arrests protein transport due to the inability of membranes to uncoat. ..
  6. Plutner H, Cox A, Pind S, Khosravi Far R, Bourne J, Schwaninger R, et al. Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. J Cell Biol. 1991;115:31-43 pubmed
    ..We suggest that rab1b may provide a common link between upstream and downstream components of the vesicular fission and fusion machinery functioning in early compartments of the secretory pathway. ..
  7. Tisdale E, Kelly C, Artalejo C. Glyceraldehyde-3-phosphate dehydrogenase interacts with Rab2 and plays an essential role in endoplasmic reticulum to Golgi transport exclusive of its glycolytic activity. J Biol Chem. 2004;279:54046-52 pubmed
    ..These data suggest that GAPDH imparts a unique function necessary for membrane trafficking from VTCs that does not require GAPDH glycolytic activity. ..
  8. Tisdale E, Artalejo C. Src-dependent aprotein kinase C iota/lambda (aPKCiota/lambda) tyrosine phosphorylation is required for aPKCiota/lambda association with Rab2 and glyceraldehyde-3-phosphate dehydrogenase on pre-golgi intermediates. J Biol Chem. 2006;281:8436-42 pubmed
  9. Tisdale E, Bourne J, Khosravi Far R, Der C, Balch W. GTP-binding mutants of rab1 and rab2 are potent inhibitors of vesicular transport from the endoplasmic reticulum to the Golgi complex. J Cell Biol. 1992;119:749-61 pubmed
    ..We suggest that at least three members of the rab family (rab1a, rab1b, and rab2) use GTP hydrolysis to regulate components of the transport machinery involved in vesicle traffic between early compartments of the secretory pathway. ..
  10. Li Y, Mi Z, Feng Y, McTiernan C, Zhou R, Robbins P, et al. Differential effects of overexpression of two forms of ephrin-A5 on neonatal rat cardiomyocytes. Am J Physiol Heart Circ Physiol. 2001;281:H2738-46 pubmed
    ..The results suggest that the two forms of ephrin-A5 share similar function while differ in regulating different sets of genes in cardiomyocytes...
  11. Kim H, Seo H, Jeong J, Lee S, Cho M, Bahk J. Molecular characterization of a rab-related small GTP binding protein cDNA from rice (Oryza sativa L. IR-36). Mol Cells. 1997;7:226-30 pubmed
    ..Deletion of a binding motif, GDTGVGKS, within an ORrab2 protein showed a significant decrease of GTP binding affinity, suggesting its important role in nucleotide binding. ..
  12. Cheung A, Chen C, Glaven R, de Graaf B, Vidali L, Hepler P, et al. Rab2 GTPase regulates vesicle trafficking between the endoplasmic reticulum and the Golgi bodies and is important to pollen tube growth. Plant Cell. 2002;14:945-62 pubmed
  13. Krijnse Locker J, Ericsson M, Rottier P, Griffiths G. Characterization of the budding compartment of mouse hepatitis virus: evidence that transport from the RER to the Golgi complex requires only one vesicular transport step. J Cell Biol. 1994;124:55-70 pubmed
  14. Sumakovic M, Hegermann J, Luo L, Husson S, Schwarze K, Olendrowitz C, et al. UNC-108/RAB-2 and its effector RIC-19 are involved in dense core vesicle maturation in Caenorhabditis elegans. J Cell Biol. 2009;186:897-914 pubmed publisher
    ..Thus, we propose that RAB-2 and its effector RIC-19 are required for neuronal DCV maturation. ..
  15. Edwards S, Charlie N, Richmond J, Hegermann J, Eimer S, Miller K. Impaired dense core vesicle maturation in Caenorhabditis elegans mutants lacking Rab2. J Cell Biol. 2009;186:881-95 pubmed publisher
    ..These results reveal how neurons solve a challenging trafficking problem using the most highly conserved animal Rab. ..
  16. Tisdale E, Azizi F, Artalejo C. Rab2 utilizes glyceraldehyde-3-phosphate dehydrogenase and protein kinase C{iota} to associate with microtubules and to recruit dynein. J Biol Chem. 2009;284:5876-84 pubmed publisher
    ..These combined results suggest a mechanism by which Rab2 controls MT and motor recruitment to vesicular tubular clusters. ..
  17. Oko R, Sutovsky P. Biogenesis of sperm perinuclear theca and its role in sperm functional competence and fertilization. J Reprod Immunol. 2009;83:2-7 pubmed publisher
    ..In this review data collected on resident perinuclear theca proteins, SubH2Bv, RAB2, PAWP and the four core somatic histones, is used to substantiate these hypotheses. ..
  18. Mountjoy J, Xu W, McLeod D, Hyndman D, Oko R. RAB2A: a major subacrosomal protein of bovine spermatozoa implicated in acrosomal biogenesis. Biol Reprod. 2008;79:223-32 pubmed publisher
    ..Our study suggests an unprecedented direction of RAB2A-mediated vesicular transport in spermatids during acrosomal biogenesis, from the trans-side of the Golgi apparatus to the nuclear envelope. ..
  19. Liu B, Bell A, Paranjpe S, Bowen W, Khillan J, Luo J, et al. Suppression of liver regeneration and hepatocyte proliferation in hepatocyte-targeted glypican 3 transgenic mice. Hepatology. 2010;52:1060-7 pubmed publisher
    ..6-fold), beta-catenin (1.7-fold), transforming growth factor beta type I (3.1-fold), nodal (1.8-fold), and yes-associated protein (1.4-fold). Changes after hepatectomy included decreased expression in several cell cycle-related genes...
  20. Urasaki N, Tokumoto M, Terauchi R, Tarora K, Chinen I, Ban Y, et al. cDNA cloning and molecular analysis of papaya small GTP-binding protein, pgp1. Genes Genet Syst. 2000;75:293-8 pubmed
    ..This result shows that PGP1 is not involved in the phytochrome-mediated signal transduction as an auxin signal transducer in stems of papaya seedlings. ..
  21. Tachibana K, Umezawa A, Takano T. Detection and characterization of mRNA and proteins encoded by human rab2 low molecular weight GTP-binding protein gene. Biochem Int. 1992;28:181-9 pubmed
    ..pp25rab2 was slightly more phosphorylated than pp24rab2. Both rab2 proteins were abundantly detected in neural PC12 and NB3 cells, and mostly pp24rab2 was produced in the other human and rodent cells. ..
  22. Culine S, Honore N, Closson V, Droz J, Extra J, Marty M, et al. A small GTP-binding protein is frequently overexpressed in peripheral blood mononuclear cells from patients with solid tumours. Eur J Cancer. 1994;30A:670-4 pubmed
    ..The precise cellular population involved as well as the potential prognostic value of this process remains to be determined. ..
  23. Biermann B, Randall S, Crowell D. Identification and isoprenylation of plant GTP-binding proteins. Plant Mol Biol. 1996;31:1021-8 pubmed
  24. Larkin J, Oswald B, McNiven M. Ethanol-induced retention of nascent proteins in rat hepatocytes is accompanied by altered distribution of the small GTP-binding protein rab2. J Clin Invest. 1996;98:2146-57 pubmed
    ..The effects of ethanol exposure on rab proteins and Golgi function are discussed. ..
  25. Bruscalupi G, Cicuzza S, Allen C, Di Croce L, Trentalance A. Estrogen stimulates intracellular traffic in the liver of Rana esculenta complex by modifying Rab protein content. Biochem Biophys Res Commun. 1998;251:301-6 pubmed
    ..An increase of the Rabs specifically involved in the exocytic pathway was observed and is probably related to the need for export of massive amounts of newly synthesized vitellogenin. ..
  26. Moore I, Diefenthal T, Zarsky V, Schell J, Palme K. A homolog of the mammalian GTPase Rab2 is present in Arabidopsis and is expressed predominantly in pollen grains and seedlings. Proc Natl Acad Sci U S A. 1997;94:762-7 pubmed
    ..Seedling germination and pollen tube growth are notable for their unusually high rates of cell wall and membrane biosynthesis. These results are consistent with a role for At-RAB2 in secretory activity. ..
  27. Tisdale E. Rab2 requires PKC iota/lambda to recruit beta-COP for vesicle formation. Traffic. 2000;1:702-12 pubmed
    ..These data suggest that Rab2 causes translocation of PKC iota/lambda to vesicular tubular clusters (VTCs), which promotes the recruitment of COPI to generate retrograde-transport vesicles. ..
  28. Wei C, Lutz R, Sinensky M, Macara I. p23rab2, a ras-like GTPase with a -GGGCC C-terminus, is isoprenylated but not detectably carboxymethylated in NIH3T3 cells. Oncogene. 1992;7:467-73 pubmed
    ..Surprisingly, unlike p21ras and p25rab3A, no carboxymethylation of p23rab2 is detectable in either the soluble or particulate fractions. ..
  29. Culine S, Honore N, Closson V, Lang P, Bertoglio J, Laroche L, et al. A CD2+ subset of non-malignant peripheral blood lymphocytes from patients with S├ęzary syndromes overexpress the low-molecular-weight GTP-binding protein Rab2. Leukemia. 1993;7:601-8 pubmed
    ..Our results provides strong evidence for the implication of a small GDP/GTP-binding protein in immunological events associated with neoplastic states. The precise cellular population involved in this process remains to be determined. ..
  30. Montpellier C, Kherrouche Z, Begue A, Stehelin D, Coll J. Rab2 nucleotide coding sequence in gallus gallus and it phylogenetic position. DNA Seq. 1995;6:37-9 pubmed
    ..The nucleic acid sequence of the chicken rab2 mRNA was determined by sequencing a full length cDNA. The phylogeny of rab2 sequences was established. ..
  31. Barr F. Purification and functional interactions of GRASP55 with Rab2. Methods Enzymol. 2005;403:391-401 pubmed
  32. Dhir V, Goulding D, Field M. TbRAB1 and TbRAB2 mediate trafficking through the early secretory pathway of Trypanosoma brucei. Mol Biochem Parasitol. 2004;137:253-65 pubmed
    ..These data indicate conservation of function for these proteins between trypanosomes and crown eukaryotes. ..
  33. O Mahony P, Oliver M. Characterization of a desiccation-responsive small GTP-binding protein (Rab2) from the desiccation-tolerant grass Sporobolus stapfianus. Plant Mol Biol. 1999;39:809-21 pubmed
    ..A possible role for Rab2 with respect to desiccation tolerance and damage repair is discussed. ..
  34. Tisdale E, Wang J, Silver R, Artalejo C. Atypical protein kinase C plays a critical role in protein transport from pre-Golgi intermediates. J Biol Chem. 2003;278:38015-21 pubmed
  35. Ni X, Ma Y, Cheng H, Jiang M, Guo L, Ji C, et al. Molecular cloning and characterization of a novel human Rab ( Rab2B) gene. J Hum Genet. 2002;47:548-51 pubmed
    ..Overexpression has been observed in colon adenocarcinoma CX-1. The Rab2B gene consists of nine exons and eight introns and is mapped to chromosome 14q11.1-14q11.2 by bioinformatics analysis. ..
  36. Benhar M, Boschwitz H, Linial M. Differential expression pattern of Rab-GDI isoforms during the parotid gland secretion cycle. Exp Cell Res. 1997;233:207-15 pubmed
    ..Our results suggest different functional roles for multiple GDI isoforms along the secretion and recovery phases in rat parotid gland. ..
  37. Culine S, Honore N, Closson V, Lang P, Bertoglio J, Tavitian A, et al. A possible role for the Ras-related Rab2 protein in the immunological events associated with hematological malignancies. Nouv Rev Fr Hematol. 1993;35:41-4 pubmed
    ..Our results provide strong evidences for the implication of a small GDP/GTP binding protein in immunological events associated with neoplastic states. The precise cellular population involved in this process remains to be determined. ..
  38. Daubenberger C, Tisdale E, Curcic M, Diaz D, Silvie O, Mazier D, et al. The N'-terminal domain of glyceraldehyde-3-phosphate dehydrogenase of the apicomplexan Plasmodium falciparum mediates GTPase Rab2-dependent recruitment to membranes. Biol Chem. 2003;384:1227-37 pubmed
    ..Together, these results suggest that pfGAPDH exerts non-glycolytic function(s) in P. falciparum, possibly including a role in vesicular transport and biogenesis of apical organelles. ..
  39. Culine S, Honore N, Tavitian A, Olofsson B. Overexpression of the ras-related rab2 gene product in peripheral blood mononuclear cells from patients with hematological and solid neoplasms. Cancer Res. 1992;52:3083-8 pubmed
    ..Circulating monocyte and lymphocyte populations from cancer patients are under analysis to correlate the Rab2 protein overexpression to a peculiar subset of cells. ..
  40. Khosravi Far R, Clark G, Abe K, Cox A, McLain T, Lutz R, et al. Ras (CXXX) and Rab (CC/CXC) prenylation signal sequences are unique and functionally distinct. J Biol Chem. 1992;267:24363-8 pubmed
    ..Finally, competition studies demonstrate that a common geranylgeranyl transferase activity is responsible for the modification of Rab proteins terminating in CC or CXC motifs. ..
  41. Tang L, Stoch S, Modlin I, Goldenring J. Identification of rab2 as a tubulovesicle-membrane-associated protein in rabbit gastric parietal cells. Biochem J. 1992;285 ( Pt 3):715-9 pubmed
    ..The results of the present study provide strong evidence that parietal cells possess a rab2 protein which is tightly associated with tubulovesicle membranes. ..
  42. He Y, Liu D, Xi D, Yang L, Tan Y, Liu Q, et al. Isolation, sequence identification and expression profile of three novel genes Rab2A, Rab3A and Rab7A from Black-boned sheep (Ovis aries). Mol Biol (Mosk). 2010;44:20-7 pubmed
    ..Our experiment is the first to provide the primary foundation for a further insight into these three sheep genes. ..
  43. Cogli L, Progida C, Lecci R, Bramato R, Kruttgen A, Bucci C. CMT2B-associated Rab7 mutants inhibit neurite outgrowth. Acta Neuropathol. 2010;120:491-501 pubmed publisher
    ..Thus, our data suggest that lowering the activity of Rab7 in neurons could be a targeted therapy for CMT2B. ..
  44. Yamada K, Ishiguro H, Ichino N, Nishii K, Sawada H, Hida T, et al. Expression levels of Rab2, a G protein, and Bag-1, a Bcl-2 binding protein are controlled by withdrawal of nicotine from cultured pheochromocytoma PC12 cells. J Neural Transm (Vienna). 2005;112:633-9 pubmed
    ..The present results suggest that such changes may also occur in individuals during abstaining from smoking and be related to the withdrawal symptoms experienced after smokers stop smoking. ..
  45. Field H, Ali B, Sherwin T, Gull K, Croft S, Field M. TbRab2p, a marker for the endoplasmic reticulum of Trypanosoma brucei, localises to the ERGIC in mammalian cells. J Cell Sci. 1999;112 ( Pt 2):147-56 pubmed
    ..TbRab2p is therefore a new ER/ERGIC marker for T. brucei. ..
  46. Fugier E, Salcedo S, de Chastellier C, Pophillat M, Muller A, Arce Gorvel V, et al. The glyceraldehyde-3-phosphate dehydrogenase and the small GTPase Rab 2 are crucial for Brucella replication. PLoS Pathog. 2009;5:e1000487 pubmed publisher
    ..abortus replication. Bacteria did not fuse with the ER and instead remained in lysosomal-associated membrane vacuoles. These results reveal an essential role for GAPDH and the small GTPase Rab 2 in B. abortus virulence within host cells...